LOCUS       NZ_PVRP01000021         1418 bp    DNA     linear   CON 22-NOV-2024
DEFINITION  Bacillus velezensis strain GBSW11 NODE_22_length_1418_cov_128.224,
            whole genome shotgun sequence.
ACCESSION   NZ_PVRP01000021
VERSION     NZ_PVRP01000021.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus velezensis
  ORGANISM  Bacillus velezensis
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales; Bacillaceae;
            Bacillus; Bacillus amyloliquefaciens group.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Borriss,R., Lasch,P., Wu,L., Wu,H. and Gao,X.
  TITLE     Genotypic and phenotypic analysis of antagonistic Bacillus spp.
            isolated from rhizosphere soil of plants in Tibet
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Borriss,R., Lasch,P., Wu,L., Wu,H. and Gao,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2018) UG, NordReet, Marienstr. 27a, Greifswald
            17489, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: A5 v. A5-2017
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 48.2x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002998915.1-RS_2024_11_20
            Annotation Date                   :: 11/20/2024 07:46:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,854
            CDSs (total)                      :: 3,748
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 106
            rRNAs                             :: 8, 10, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 9, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 42 of 76
            Pseudo Genes (incomplete)         :: 45 of 76
            Pseudo Genes (internal stop)      :: 7 of 76
            Pseudo Genes (multiple problems)  :: 16 of 76
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:10:33
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 1418
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            PVRP01000021.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1418
                     /altitude="4000 m"
                     /collection_date="2005"
                     /db_xref="taxon:492670"
                     /geo_loc_name="China: Tibet"
                     /isolation_source="soil"
                     /mol_type="genomic DNA"
                     /organism="Bacillus velezensis"
                     /strain="GBSW11"
                     /submitter_seqid="NODE_22_length_1418_cov_128.224"
     gene            complement(<1..>1418)
                     /locus_tag="C6355_RS19280"
                     /old_locus_tag="C6355_19270"
     region          1..1418
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..1418
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..1418
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:1418](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..1418)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS             complement(<1..1418)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: PCP (166-234). E-value: 6.1e-26. Score:
                     82.4. Matches aSDomain: nrpspksdomains_C6355_RS19280_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (249-471). E-value:
                     4.7e-73. Score: 237.8. Matches aSDomain:
                     nrpspksdomains_C6355_RS19280_Condensation_LCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014305115.1"
                     /locus_tag="C6355_RS19280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6355_19270"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_142396304.1"
                     /sec_met_domain="AMP-binding (E-value: 2.5e-35, bitscore:
                     107.8, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 3.5e-18, bitscore:
                     52.2, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 6.1e-67, bitscore:
                     211.9, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELYIAGTGVARGYLNRSELTEERFLDDPFYPGERMYQTGDIARW
                     TEDGLVEWLGRSDGQVKVRGYRIEPGEIEAAIRCIDGIREAAVTARTEHGETELYAYIE
                     GRESDDVRAELAARLPVYMMPAQFIEVSEWPVTPSGKLDRRALPAPDGAADRQAYTAPR
                     NVTEMKLCALWEEVLKNGPVGIRDHFFERGGHSLKATALVSRIAKEFGVQVPLQDIFAR
                     PTVEELASVIQDLEESPYEAIQPAQKQDTYPVSSAQKRMYVLQQLEDGGVGYNMPAVLE
                     LTGPLDRIRLEETFRRLVERHESLRTSFETGPDGEPVQRIHDSVPFQLDEAESADAFVR
                     PFCLEEAPLFRAALVKESDERHLLLTDMHHIISDGVSVNTLIKEFGELYAGRSLAPMRL
                     QYKDYAVWQRSFQEKEGYQKQEAYWLKRLEGELPVLELPADKPRPAVRSFAGGSVSCAL
                     DAETASGLHRIARD"
     aSDomain        complement(6..671)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_C6355_RS19280_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="4.70E-73"
                     /label="C6355_RS19280_Condensation_LCL.1"
                     /locus_tag="C6355_RS19280"
                     /protein_end="471"
                     /protein_start="249"
                     /score="237.8"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIISDG"
                     /tool="antismash"
                     /translation="DTYPVSSAQKRMYVLQQLEDGGVGYNMPAVLELTGPLDRIRLEET
                     FRRLVERHESLRTSFETGPDGEPVQRIHDSVPFQLDEAESADAFVRPFCLEEAPLFRAA
                     LVKESDERHLLLTDMHHIISDGVSVNTLIKEFGELYAGRSLAPMRLQYKDYAVWQRSFQ
                     EKEGYQKQEAYWLKRLEGELPVLELPADKPRPAVRSFAGGSVSCALDAETASGLHRIAR
                     "
     CDS_motif       complement(189..221)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C6355_RS19280_0006"
                     /evalue="8.70E-06"
                     /label="C4_LCL_164-176"
                     /locus_tag="C6355_RS19280"
                     /protein_end="410"
                     /protein_start="399"
                     /score="18.2"
                     /tool="antismash"
                     /translation="QYKDYAVWQRS"
     CDS_motif       complement(252..317)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C6355_RS19280_0005"
                     /evalue="3.70E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="C6355_RS19280"
                     /protein_end="389"
                     /protein_start="367"
                     /score="29.1"
                     /tool="antismash"
                     /translation="MHHIISDGVSVNTLIKEFGELY"
     CDS_motif       complement(492..599)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C6355_RS19280_0004"
                     /evalue="6.30E-17"
                     /label="C2_DCL_024-062"
                     /locus_tag="C6355_RS19280"
                     /protein_end="309"
                     /protein_start="273"
                     /score="53.8"
                     /tool="antismash"
                     /translation="YNMPAVLELTGPLDRIRLEETFRRLVERHESLRTSF"
     aSDomain        complement(717..920)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_C6355_RS19280_PCP.1"
                     /evalue="6.10E-26"
                     /label="C6355_RS19280_PCP.1"
                     /locus_tag="C6355_RS19280"
                     /protein_end="234"
                     /protein_start="166"
                     /score="82.4"
                     /tool="antismash"
                     /translation="EMKLCALWEEVLKNGPVGIRDHFFERGGHSLKATALVSRIAKEFG
                     VQVPLQDIFARPTVEELASVIQD"
     CDS_motif       complement(1197..1262)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C6355_RS19280_0003"
                     /evalue="5.90E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="C6355_RS19280"
                     /protein_end="74"
                     /protein_start="52"
                     /score="37.5"
                     /tool="antismash"
                     /translation="WLGRSDGQVKVRGYRIEPGEIE"
     CDS_motif       complement(1278..1301)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C6355_RS19280_0002"
                     /evalue="2.30E+01"
                     /label="C4_LCL_164-176"
                     /locus_tag="C6355_RS19280"
                     /protein_end="47"
                     /protein_start="39"
                     /score="-1.5"
                     /tool="antismash"
                     /translation="GDIARWTE"
     CDS_motif       complement(1344..1415)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C6355_RS19280_0001"
                     /evalue="3.30E-13"
                     /label="NRPS-A_a6"
                     /locus_tag="C6355_RS19280"
                     /protein_end="25"
                     /protein_start="1"
                     /score="41.8"
                     /tool="antismash"
                     /translation="ELYIAGTGVARGYLNRSELTEERF"
ORIGIN
        1 tgatcccggg cgatgcggtg aaggccggat gccgtttcag cgtcaagcgc gcacgatacg
       61 ctgccgcccg caaagcttct gacagccgga cggggcttgt cagccggaag ctcaagcacc
      121 ggaagctctc cttcaagccg cttcagccaa taggcttcct gcttctgata cccttctttc
      181 tcctgaaacg accgctgcca gacggcatag tctttatatt gaaggcgcat cggcgcaagc
      241 gaacggcctg cgtacagctc gccgaattct ttaatcaagg tgttgacgga cacaccgtca
      301 gaaataatat gatgcatatc cgttaataaa agatgacgtt cgtccgattc tttcacgagg
      361 gccgcacgga agagcggcgc ttcctccaga caaaacggcc ggacgaatgc atcagctgac
      421 tcggcctcat ccagctgaaa cggcacgctg tcatggatgc gctgcaccgg ttcgccatca
      481 ggccccgttt caaaggacgt tctgagcgac tcatgccgct ccacaagccg ccggaacgtc
      541 tcctccagcc ggatgcggtc gagcgggccc gtcagttcca gcacagccgg catattgtag
      601 ccgacaccgc cgtcttcaag ctgctggagc acatacatcc gtttctgcgc cgaagacacc
      661 gggtaggtgt cctgtttttg cgcaggctga atcgcttcat acgggctctc ttccaagtcc
      721 tgaatgaccg aagcgagctc ttccaccgtc ggccgggcga agatgtcttg taacggcacc
      781 tgaacaccga actctttggc gatgcgggag acgagagccg tcgcttttaa cgaatggccg
      841 ccccgctcaa aaaagtgatc ccggatgccg acggggccgt ttttcagcac ctcttcccaa
      901 agggcgcaaa gcttcatttc cgtgacgttc cgcggggcgg tgtacgcctg tctgtctgcc
      961 gctccgtcgg gagccggaag cgcgcggcgg tcaagctttc cgctcggcgt gaccggccat
     1021 tcgctcacct ctataaactg agccggcatc atgtagaccg gaaggcgtgc cgcaagctcc
     1081 gcccgcacgt catcgctttc ccgtccttcg atataggcgt aaagctcggt ttcgccgtgt
     1141 tcggtccggg cggtgaccgc cgcctcacga atgccgtcga tgcaccggat cgccgcttcg
     1201 atttctcccg gttcaatccg gtagccgcgg actttcactt gtccgtctga acggccgagc
     1261 cattcgacta ggccgtcttc tgtccagcgt gcgatatcgc ccgtctgata catgcgctct
     1321 cccggataaa acggatcatc gagaaaacgc tcctccgtca gctcggaccg gttcagatag
     1381 ccccgggcca cccctgtccc cgcgatgtac agctctcc
//
