LOCUS       NZ_PVRP01000019         7215 bp    DNA     linear   CON 22-NOV-2024
DEFINITION  Bacillus velezensis strain GBSW11 NODE_20_length_7215_cov_41.2585,
            whole genome shotgun sequence.
ACCESSION   NZ_PVRP01000019
VERSION     NZ_PVRP01000019.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus velezensis
  ORGANISM  Bacillus velezensis
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales; Bacillaceae;
            Bacillus; Bacillus amyloliquefaciens group.
REFERENCE   1  (bases 1 to 7215)
  AUTHORS   Borriss,R., Lasch,P., Wu,L., Wu,H. and Gao,X.
  TITLE     Genotypic and phenotypic analysis of antagonistic Bacillus spp.
            isolated from rhizosphere soil of plants in Tibet
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7215)
  AUTHORS   Borriss,R., Lasch,P., Wu,L., Wu,H. and Gao,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2018) UG, NordReet, Marienstr. 27a, Greifswald
            17489, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: A5 v. A5-2017
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 48.2x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002998915.1-RS_2024_11_20
            Annotation Date                   :: 11/20/2024 07:46:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,854
            CDSs (total)                      :: 3,748
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 106
            rRNAs                             :: 8, 10, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 9, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 42 of 76
            Pseudo Genes (incomplete)         :: 45 of 76
            Pseudo Genes (internal stop)      :: 7 of 76
            Pseudo Genes (multiple problems)  :: 16 of 76
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:10:33
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 7215
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            PVRP01000019.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7215
                     /altitude="4000 m"
                     /collection_date="2005"
                     /db_xref="taxon:492670"
                     /geo_loc_name="China: Tibet"
                     /isolation_source="soil"
                     /mol_type="genomic DNA"
                     /organism="Bacillus velezensis"
                     /strain="GBSW11"
                     /submitter_seqid="NODE_20_length_7215_cov_41.2585"
     region          1..7215
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..7215
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..7215
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[5363:>7215](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5364..>7215
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     gene            complement(147..689)
                     /locus_tag="C6355_RS19240"
                     /old_locus_tag="C6355_19230"
     CDS             complement(147..689)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389720.2"
                     /locus_tag="C6355_RS19240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6355_19230"
                     /product="IseA DL-endopeptidase inhibitor family protein"
                     /protein_id="WP_014418077.1"
                     /transl_table=11
                     /translation="MKKWLLFLTTITLILSLGTAATAKNAPNDLTQKQALQLALSAREH
                     FWNTMSGGALKSNANCTSEPFEYQNLQYVFMCKDLGTKAKAVKYLTPAFTKQAIDKGLK
                     DYHFTVKDGKLAVPVGDGDNLLNWKKAKMTLLSKKGSVQTYRFTVPTLDGSPSAKRDVT
                     FVKENNVWKVNQFDAVI"
     misc_feature    complement(832..847)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 22.41"
                     /tool="antismash"
     gene            918..2306
                     /locus_tag="C6355_RS19245"
                     /old_locus_tag="C6355_19235"
     CDS             918..2306
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1086: MATE efflux
                     family protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153854.1"
                     /locus_tag="C6355_RS19245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6355_19235"
                     /product="MATE family efflux transporter"
                     /protein_id="WP_032866217.1"
                     /transl_table=11
                     /translation="MEVQDHLETAEGRGQSVWRSMSLFLVPLLLSNVLQSVGQLVGMVF
                     VGRWLGVDALAAVSSFFPLFFLLISFTIGIGSGSSILIGQAYGAKNEERMKAIVGTTLT
                     FTFMLGVVLAVVGSVFTLDILRLMGTPENVIDVSVRYARILFCAMPLMFLYFAYTTFLR
                     GTGDSKTPFYTLIVSTVINIGLLPLLILGLFGLPKFGIYGSAYATVISNIVTFIVLMIY
                     LRKRKHPLAFDRTALRSLKMDKKLLALLLRLGIPSSINMILVSLSEIAVISFVNHYGSN
                     ATAAYGVVNQVASYVQMPAVSLGIAVSIFAAQSIGANQFGRLKQVIKVGIGLNYLIGGV
                     LIVLIYTFSRQILALFLTDQDTLHIARNLIMITLWSYLIFGHAQIVSATMRASGTVLWP
                     TIISIFAIWGIEVPVAFILSHYTNLGILGIWVGYPAAFVVSLLLQYGYYRFVWKKKQIT
                     RLIQ"
     gene            2422..3390
                     /locus_tag="C6355_RS19250"
                     /old_locus_tag="C6355_19240"
     CDS             2422..3390
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153855.1"
                     /locus_tag="C6355_RS19250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6355_19240"
                     /product="aldose 1-epimerase"
                     /protein_id="WP_106080476.1"
                     /transl_table=11
                     /translation="MTIEHTTYLGASAIKAETDQAECIIVPEWGSNVISLRDKRTGMRL
                     LREPETAEQFRKKPMLYGIPVLFPPNRITDGTFRFDGRAYQFEINEEDKHNHIHGFLHN
                     QHWKAAAAKEEGDGMTVETEIDLSKIPRVHKQFPHDVIVRMTYTLKDNVFTQRALIMNK
                     GTEAFPWGLGYHSTFVFNEERSRLFLTADRMWELNERFVPTGRLLDVPDKEAFRQGVSL
                     KNKQFDDVFLSSGRNGGNNEAVIYHPDEQLSVLYQADMSFKHWVVYNGDGKQGFVSLEP
                     YTSVTDAFNLNQPASLTGLQILKPGEEAAASSRITILHDEE"
     gene            3409..4884
                     /gene="dacB"
                     /locus_tag="C6355_RS19255"
                     /old_locus_tag="C6355_19245"
     CDS             3409..4884
                     /EC_number="3.4.16.4"
                     /GO_function="GO:0004175 - endopeptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA]"
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="dacB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015388145.1"
                     /locus_tag="C6355_RS19255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6355_19245"
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="WP_106080475.1"
                     /transl_table=11
                     /translation="MKTYVKRLSAVILLLVIAAVPYIDEAAKAAEQKNTLQKELELILD
                     EEPALKGVSAGVSVRSAKTGEVLFGSREDMRLRPASLMKLLTASAALSVLGEDYTFKTE
                     VRADGAVKGKQLRGNLYLRGKGDPTLLASAFEKMAKQVKARGIHVIKGDLVGDDSWYDD
                     TGYSVDLPWSDEGQYYGAQVSALTASPNEDYDTGTVIAEISPAKQPGKKPRISISPHTD
                     VVRVKNEVKTVASDEAKELTVEREHGGNVITIKGTIPAGSAKAREWAAVWDPSSYALDL
                     WKQALTEQGITVKGKIRTGRMPHRTQLVTSRTSMPLSELLIPFMKLSNNGHAEILIKEM
                     GKVKKGEGSWEKGLDVMKSELKSFGLNPDKLIARDGSGVSHINGVSAGQIGELLYAVQK
                     EKWYPAFLRSLPVAGASDRMTGGTLRNRLKNTPAEGKIKAKTGSLTSVSSIAGYADTKT
                     GDTLIFSVLQNGLLDEDDGKDIEDKIAVVLANQ"
     misc_feature    5033..5046
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 21.75"
                     /tool="antismash"
     misc_feature    5035..5048
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 20.79"
                     /tool="antismash"
     gene            5364..>7215
                     /locus_tag="C6355_RS19265"
                     /old_locus_tag="C6355_19255"
     CDS             5364..>7215
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Condensation_Starter (3-299). E-value:
                     1.5e-85. Score: 279.1. Matches aSDomain:
                     nrpspksdomains_C6355_RS19265_Condensation_Starter.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014418073.1"
                     /locus_tag="C6355_RS19265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6355_19255"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_142396303.1"
                     /sec_met_domain="Condensation (E-value: 5.9e-69, bitscore:
                     220.8, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 5.3e-40, bitscore:
                     125.5, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MENTVYSLTHAQRRVWFTELLEPGTSICNLAACVKFRGDIDFDVL
                     RHALDFSISQNDSLRFQLTEGDGSEPQLYLAGHRPISLETVDFTHTDQAERDAWIDTQT
                     RVPFKLFHSPLYQFTLLVMSDEEVWLYSKFHHIIMDGISLNLLGNQLIDIYQKIMRSED
                     LTEHHRPSYLSYMEKEQQYLQSSRFQKDRSFWTETYRTVPEHLSLAERTSHLRQSTAAS
                     RDTITLSHSLEQSIRLFCKENNISIISLFMASLYICISRLTAKKDIAIGTYYGNRGSRL
                     EKDMLGMFVSTLPIRMTADPDAEFLSFVRSVGKEQLSVMRHQKYPYNLFVNELRQTHHD
                     LQNIIGISMQYQPLEWRKADGFDYETAMFFSGHTANELSILIKERTDTGLIELNFDYQS
                     ALFTETSIKRIQTHLLTIVENAVRNPDRLIRHIDMADEKEKKRVLSSFNRTEAVEPPAP
                     TLHGLFTRRAALSPHRPALCFPGGMLTYAELDQYTDKLAVWLRQKGVRKESMVGVLAER
                     SPEMVVSVLAVLKAGGAYVPLDPEYPEDRLRYMLDDCGACLLLAQPGLSVSGFSGETLE
                     VSLSALTGETETGAVSEEADSDSLAYIIYTSGSTGTPKGVA"
     aSDomain        5373..6260
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_C6355_RS19265_Condensation_Start
                     er.1"
                     /domain_subtypes="Condensation_Starter"
                     /evalue="1.50E-85"
                     /label="C6355_RS19265_Condensation_Starter.1"
                     /locus_tag="C6355_RS19265"
                     /protein_end="299"
                     /protein_start="3"
                     /score="279.1"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIIMDG"
                     /tool="antismash"
                     /translation="TVYSLTHAQRRVWFTELLEPGTSICNLAACVKFRGDIDFDVLRHA
                     LDFSISQNDSLRFQLTEGDGSEPQLYLAGHRPISLETVDFTHTDQAERDAWIDTQTRVP
                     FKLFHSPLYQFTLLVMSDEEVWLYSKFHHIIMDGISLNLLGNQLIDIYQKIMRSEDLTE
                     HHRPSYLSYMEKEQQYLQSSRFQKDRSFWTETYRTVPEHLSLAERTSHLRQSTAASRDT
                     ITLSHSLEQSIRLFCKENNISIISLFMASLYICISRLTAKKDIAIGTYYGNRGSRLEKD
                     MLGMFVSTLPIRMTA"
     CDS_motif       5379..5417
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C6355_RS19265_0001"
                     /evalue="3.40E-03"
                     /label="C1_starter_004-017"
                     /locus_tag="C6355_RS19265"
                     /protein_end="18"
                     /protein_start="5"
                     /score="10.3"
                     /tool="antismash"
                     /translation="YSLTHAQRRVWFT"
     CDS_motif       5439..5558
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C6355_RS19265_0002"
                     /evalue="1.20E-11"
                     /label="C2_starter_024-063"
                     /locus_tag="C6355_RS19265"
                     /protein_end="65"
                     /protein_start="25"
                     /score="37.2"
                     /tool="antismash"
                     /translation="SICNLAACVKFRGDIDFDVLRHALDFSISQNDSLRFQLTE"
     CDS_motif       5763..5828
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C6355_RS19265_0003"
                     /evalue="3.60E-08"
                     /label="C3_starter_134-155"
                     /locus_tag="C6355_RS19265"
                     /protein_end="155"
                     /protein_start="133"
                     /score="26.2"
                     /tool="antismash"
                     /translation="FHHIIMDGISLNLLGNQLIDIY"
     CDS_motif       6162..6251
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C6355_RS19265_0004"
                     /evalue="6.00E-09"
                     /label="C5_starter_265-294"
                     /locus_tag="C6355_RS19265"
                     /protein_end="296"
                     /protein_start="266"
                     /score="28.3"
                     /tool="antismash"
                     /translation="DIAIGTYYGNRGSRLEKDMLGMFVSTLPIR"
     CDS_motif       6303..6416
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C6355_RS19265_0005"
                     /evalue="5.80E-08"
                     /label="C67_starter_14fromHMM"
                     /locus_tag="C6355_RS19265"
                     /protein_end="351"
                     /protein_start="313"
                     /score="25.3"
                     /tool="antismash"
                     /translation="KEQLSVMRHQKYPYNLFVNELRQTHHDLQNIIGISMQY"
     CDS_motif       6939..6980
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C6355_RS19265_0006"
                     /evalue="1.10E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="C6355_RS19265"
                     /protein_end="539"
                     /protein_start="525"
                     /score="21.5"
                     /tool="antismash"
                     /translation="LAVLKAGGAYVPLD"
     CDS_motif       7164..7214
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C6355_RS19265_0007"
                     /evalue="1.00E-08"
                     /label="NRPS-A_a3"
                     /locus_tag="C6355_RS19265"
                     /protein_end="617"
                     /protein_start="600"
                     /score="27.3"
                     /tool="antismash"
                     /translation="AYIIYTSGSTGTPKGVA"
ORIGIN
        1 cacttcaacc ggataactca ctcttgtccc catagaaaaa acacctccaa gtctaatttc
       61 ggataacaat catccgtatt caaacttgaa ggtgttttta tttgtctcat cttatggggt
      121 cagtccctaa gcaagcgggc tttttcttat atgacagcat caaattgatt gactttccaa
      181 acgttgtttt cttttacgaa tgttacatcg cgttttgctg acggagaacc gtcaagagtc
      241 ggtacagtga agcggtatgt ttggacgctt ccttttttag aaagaagggt catttttgct
      301 tttttccaat tcaggaggtt atctccgtcg ccgaccggaa cagccaattt gccgtctttt
      361 acagtgaagt ggtaatcttt gagccctttg tcaatcgctt gctttgtaaa agcgggtgtc
      421 agatatttta cggctttcgc ttttgttccg aggtctttgc acataaatac atattgcaga
      481 ttttgatatt caaacggttc agatgtgcaa tttgcgtttg atttaagcgc accgccgctc
      541 attgtattcc agaaatgttc tctcgctgac agggccagct gaagtgcttg tttttgtgta
      601 aggtcattag gtgcattttt tgcagtcgcc gcagtgccca atgatagaat caatgtaata
      661 gtagtgagga ataatagcca ttttttcatg atataatctc tccttcaatc aatctctttc
      721 tcttatgtta caaatgttac aataattacg gtaagattac aatacctttt cagaaatatt
      781 ttccggactt tttattttta tggtttattt ccatgtaaga cgtgagaaaa agataaagag
      841 tttcattgaa caaaaggttt ttacaagctg tctgctgaat aataaaatgg aagaaaatca
      901 aaaaaggagt cctacatatg gaagtacaag atcatttaga aacagcggaa ggccgcggcc
      961 agtcagtctg gcgatcgatg tcactgtttt tagtgccgct gcttttaagc aatgtattgc
     1021 aatctgtcgg ccaattggtc ggaatggtat ttgtcggaag gtggctcgga gtcgatgcgc
     1081 ttgccgctgt atcgtctttt tttccgctgt tttttctgct gatctccttt acgatcggaa
     1141 tcggttcggg gagttccatt ctgatcgggc aggcttacgg cgcgaaaaac gaagagagga
     1201 tgaaggcgat tgtcggcact acactgactt ttacctttat gctcggtgtt gtcctggcag
     1261 ttgtcggcag tgtgtttacg cttgatattc tgcggctgat gggaacgccg gaaaacgtaa
     1321 tagatgtcag tgtccgatat gcgcgcattt tgttttgcgc aatgccgctg atgtttttat
     1381 attttgcgta tacgacattt ttacgcggaa cgggagattc aaagaccccg ttttatacgt
     1441 taatcgtcag tacagtcata aacatcgggc tcttgccctt attgattctc gggcttttcg
     1501 gacttccgaa gttcggcatt tacggttcgg cgtatgcaac cgtgatttca aacatcgtga
     1561 catttatcgt actcatgatt tatttgcgga aaaggaaaca tccattggca tttgaccgga
     1621 ctgcgctccg ttctcttaaa atggacaaaa agctgctcgc gcttttactc cgtctcggta
     1681 ttccgtcgag catcaatatg attctcgtgt cactttctga aattgcggtc atttcatttg
     1741 tcaatcatta cggatcaaac gcgacggctg cttacggggt cgtcaaccaa gtggcgagtt
     1801 atgtgcagat gccggcggta agcctcggta tcgccgtttc catttttgcc gcccaatcca
     1861 ttggcgccaa tcaattcggc cggctgaaac aggtcatcaa agtcggcatc ggcctgaatt
     1921 atttgatcgg cggggttttg attgtgctga tttacacctt ctcccggcag atacttgctt
     1981 tgtttttaac agatcaggat acactccaca ttgcccgcaa tctgattatg attacccttt
     2041 ggagctattt gattttcggt cacgctcaga tcgtttcagc gacaatgcgg gcgagcggta
     2101 cggtgttatg gccgacgatt atcagtattt ttgcgatttg gggaattgaa gtgcctgtgg
     2161 cctttattct ttctcattat acgaatctcg gcattctcgg catttgggtc ggttatccgg
     2221 cggcttttgt cgtcagcctg ctgctgcaat acggatatta ccggtttgta tggaagaaaa
     2281 aacagattac ccggctgatt caataaggtt ttaccgcaca tcgctcagtt catacgggcg
     2341 atgttttttt tagagaagga aaatgagttt agaaaatgga attcctatac atgctagaaa
     2401 acaaggaaag ggcaggtgct tatgacgatt gaacatacga cttatttagg ggcttcagcc
     2461 ataaaagcgg agactgatca ggctgaatgc atcattgtgc cggagtgggg atcaaatgtc
     2521 atatcgcttc gggataaaag aaccggtatg cggcttctga gagaaccgga aactgcggag
     2581 caattccgca aaaaaccgat gctgtacggc attcccgttt tattcccgcc gaaccggatt
     2641 acggatggga cgtttcgttt tgacggccgc gcgtatcaat ttgagataaa cgaagaggat
     2701 aaacataatc atattcacgg ctttcttcac aatcagcatt ggaaagcggc ggcagcgaaa
     2761 gaagaggggg atggaatgac agtcgaaaca gaaattgatc tttcaaagat tccccgtgtt
     2821 cataaacaat ttccgcacga tgtcattgtc cggatgacct atacattaaa agacaatgtg
     2881 tttacgcagc gggcgctgat tatgaataag ggaacggagg cttttccttg ggggctcggc
     2941 tatcattcaa cttttgtttt caatgaagag cgatcccgtc tatttttgac ggctgaccgc
     3001 atgtgggaat taaatgaacg tttcgtgccg acgggccgtt tattggatgt gcctgataaa
     3061 gaagcgttcc gtcagggggt gtctttgaaa aataaacagt ttgacgatgt ttttctttct
     3121 tccggacgga atggcggcaa taacgaagct gttatttatc atccggatga gcaattatcc
     3181 gtgctgtatc aagcagacat gtctttcaag cattgggtgg tatataacgg tgacggaaag
     3241 caagggtttg tcagtcttga gccttatact tccgttacag atgcatttaa tctgaatcag
     3301 cctgcatcac tgacagggct tcagattctg aaaccgggcg aagaagcggc tgcgtcatcc
     3361 aggatcacca ttttgcatga cgaagaatag gaaagaggcg ggataacatt gaaaacatat
     3421 gtgaaacgct tgagcgcggt gattctgctt ttggtcattg ccgcagttcc ttatattgat
     3481 gaggcggcta aggcggctga acaaaaaaat actttgcaaa aagagctgga actcattctg
     3541 gatgaagaac ctgctttaaa gggagtttca gcgggcgtca gtgtccggtc agcaaaaaca
     3601 ggggaggtgc tgttcggcag ccgggaagat atgcggctga ggcctgcatc actgatgaag
     3661 ctgctgacgg catctgcagc gctttccgtt ctcggagaag attacacctt taagacagaa
     3721 gtacgggcag acggcgccgt aaaaggaaaa cagctgcgcg gaaaccttta tctcagggga
     3781 aaaggtgatc cgaccctatt ggcttctgct tttgagaaga tggctaaaca ggtcaaagcc
     3841 agaggcatcc atgtcattaa aggagatctg gtcggggatg attcgtggta tgacgatacc
     3901 gggtattcag ttgatcttcc gtggagtgat gaaggccaat attatggcgc gcaagtatca
     3961 gctcttaccg cttccccaaa tgaggattat gataccggaa cggtgattgc ggaaatcagc
     4021 ccggcaaaac agcccggtaa aaagccgcgc atttcgattt ccccccatac ggacgtcgtt
     4081 cgtgtgaaaa atgaagtgaa aaccgtcgct tctgatgaag cgaaagaatt gacggttgaa
     4141 cgggagcacg gcggcaatgt gattacgata aagggaacca tacctgccgg ctcggcaaag
     4201 gcaagagaat gggcggcggt gtgggatccg tcgtcctatg ctttagattt atggaaacaa
     4261 gcgcttactg aacagggtat aactgtaaaa gggaaaatca gaacagggcg aatgccgcac
     4321 cgcacccagc ttgtcacatc acggacatcc atgcctcttt cagaattatt gatcccgttt
     4381 atgaaactga gcaacaacgg gcacgccgaa attctgatca aggaaatggg aaaagtgaaa
     4441 aaaggggagg gcagctggga aaagggcttg gacgtgatga aatcggagct caaatcgttc
     4501 gggctcaatc cggataaatt gattgcaaga gacggatccg gcgtttctca tattaacggc
     4561 gtatccgccg gccaaatcgg agagcttttg tatgcggttc agaaagaaaa gtggtacccg
     4621 gctttccttc gttcgctccc tgttgccggc gcaagcgaca gaatgacggg aggaaccctt
     4681 cggaaccggc tgaagaatac tccggcagaa ggcaaaatta aagcgaaaac cgggtcacta
     4741 acatcagtca gttcgattgc cggctatgca gacacaaaaa caggagatac gcttattttt
     4801 tccgtgcttc aaaacgggct gcttgacgaa gatgacggaa aagacattga agacaaaatc
     4861 gcagtcgtgc tggcaaatca ataagaaggg ccgaatcctt agaggattcg gctttttttg
     4921 atgtctgtaa aacattatat tgcgattatg aatcaatctt ttcaaaaagt aatatcggca
     4981 atatccttcc ttttttttac ataccgcttt cttacatgtt caccggttat tcatttttta
     5041 acataaaact tcttttcggg aggaaaacct ttttaaaagc gcaattaatt atacatttta
     5101 ttccttatta tagtgtttaa gaaccagttt atagtatgca tacataccgt tttacatggt
     5161 ataaagatta tagaaatgaa gaagtgaagt gaggaatata tactatattc taaaaaatgt
     5221 tagagaaaat ttaatttttt attgtgattt ctaaaatatg ttttaaaata atgcttatat
     5281 ggatgattaa gtcatatgct gtcgaaattt aattgtaatt atttcacttt cttatcctct
     5341 taaattgaag cggagggatt ccattggaga acactgttta ttctttaact cacgcacaaa
     5401 gaagagtgtg gtttacggag ctgctcgaac cgggcacaag tatctgcaat ctcgcggcat
     5461 gcgtcaaatt caggggggac atcgattttg acgtactgcg ccatgcttta gatttttcta
     5521 tctcgcaaaa tgattcgctc aggtttcaac tgacagaagg ggacggatca gaaccccagc
     5581 tgtatcttgc ggggcatcgg ccgatttctc tagagactgt tgattttact catactgatc
     5641 aggcagagcg ggacgcatgg attgacacgc agacccgtgt tccgttcaag ctgtttcatt
     5701 cacctctgta tcaatttact ctgcttgtca tgtcagacga agaagtttgg ctttattcaa
     5761 aatttcacca tattatcatg gacggcatct cgctcaacct gctcggcaac cagctcattg
     5821 atatctacca aaagatcatg cggagcgagg atttaactga acaccacaga ccttcctatc
     5881 tcagctacat ggaaaaagaa cagcaatact tacagtcttc ccgttttcaa aaagaccgtt
     5941 cattttggac tgaaacatac cgaaccgttc cggaacacct ttcactggcg gaacgaacct
     6001 ctcatcttag acaaagcact gcggcttcca gagatacaat cactctttct cactccttag
     6061 aacaatcaat ccggcttttt tgcaaagaaa ataatatcag catcatctct ctgtttatgg
     6121 cttcgttata catatgcatc agccgtctga cggctaaaaa ggatattgcc atcggcactt
     6181 attacggaaa ccggggctcc aggctcgaaa aggatatgct cggcatgttc gtcagcacac
     6241 tgccgatcag aatgacggca gatcctgatg cagagttcct gtcctttgtc cgcagcgtag
     6301 gaaaagagca gctgtctgtt atgcggcacc agaaatatcc ttacaatctt tttgtgaatg
     6361 aattaaggca gactcatcat gacctgcaaa atatcatcgg tatttcgatg cagtatcagc
     6421 cgcttgagtg gcgtaaggca gacggtttcg attatgaaac ggccatgttt ttcagcggac
     6481 ataccgcaaa cgagctctcc attttaatta aggagcggac agatacggga ttgattgagc
     6541 tgaattttga ttaccaatcc gctttattta ctgaaacatc aattaaacgc attcaaactc
     6601 atttgctgac gatcgttgaa aatgccgtta gaaatccaga ccggctgatt cggcatattg
     6661 atatggcgga tgaaaaagaa aagaaacggg tgttatcttc gtttaaccgt acagaagcgg
     6721 ttgaaccgcc ggctccgacg cttcacgggc tgttcacccg ccgtgccgcg ctttcgccgc
     6781 atcgtccggc gctttgtttt ccgggcggta tgctgacgta cgccgagctt gaccagtaca
     6841 cagacaaact cgctgtttgg ctccgacaaa aaggcgtccg caaagaaagc atggtcggcg
     6901 tgctcgctga acgctcgccg gagatggtgg tttcagtgct tgccgtgtta aaagcgggcg
     6961 gcgcctacgt gccgcttgac ccggagtatc ctgaggacag gctgcgttat atgctcgatg
     7021 actgcggcgc ttgtctcctg cttgcacagc cggggttatc cgtttccggt ttttccggcg
     7081 agacgcttga agtgtctctt tctgctctga caggcgagac ggagacaggg gctgtttctg
     7141 aagaagcgga tagcgattcg ctcgcttata tcatttatac gtccggttcg acgggaacac
     7201 cgaagggagt ggctg
//
