LOCUS       NZ_PVRL01000042         2800 bp    DNA     linear   CON 21-MAY-2024
DEFINITION  Bacillus thuringiensis strain NMTD81
            NODE_42_length_2800_cov_20.4527, whole genome shotgun sequence.
ACCESSION   NZ_PVRL01000042
VERSION     NZ_PVRL01000042.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Borriss,R., Lasch,P., Wu,L., W,H. and Gao,X.
  TITLE     Genotypic and phenotypic analysis of antagonistic Bacillus spp.
            isolated from rhizosphere soil of plants in Tibet
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Borriss,R., Lasch,P., Wu,L., W,H. and Gao,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2018) UG, NordReet, Marienstr. 27a, Greifswald
            17489, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: A5 v. A5-2017
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 14.5x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002998815.1-RS_2024_05_21
            Annotation Date                   :: 05/21/2024 01:45:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,177
            CDSs (total)                      :: 6,068
            Genes (coding)                    :: 5,840
            CDSs (with protein)               :: 5,840
            Genes (RNA)                       :: 109
            rRNAs                             :: 11, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 1 (5S, 23S)
            partial rRNAs                     :: 6, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 228
            CDSs (without protein)            :: 228
            Pseudo Genes (ambiguous residues) :: 0 of 228
            Pseudo Genes (frameshifted)       :: 100 of 228
            Pseudo Genes (incomplete)         :: 142 of 228
            Pseudo Genes (internal stop)      :: 67 of 228
            Pseudo Genes (multiple problems)  :: 63 of 228
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:59:29
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 2800
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            PVRL01000042.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2800
                     /altitude="4000 m"
                     /collection_date="2005"
                     /country="China: Tibet"
                     /db_xref="taxon:1428"
                     /isolation_source="soil"
                     /mol_type="genomic DNA"
                     /organism="Bacillus thuringiensis"
                     /strain="NMTD81"
                     /submitter_seqid="NODE_42_length_2800_cov_20.4527"
     region          1..2800
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..2800
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..2800
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[84:>2800](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      85..>2800
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     aSDomain        85..279
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_C6351_RS32010_PCP.1"
                     /evalue="8.90E-21"
                     /label="C6351_RS32010_PCP.1"
                     /locus_tag="C6351_RS32010"
                     /protein_end="65"
                     /protein_start="0"
                     /score="65.9"
                     /tool="antismash"
                     /translation="MVEIWSEVLGIDSSAIGINDNFFELGGHSLLATKITSRLGVDFSI
                     LVQIRDIFEYPTIKHFSKRL"
     gene            85..>2800
                     /locus_tag="C6351_RS32010"
                     /old_locus_tag="C6351_32015"
     CDS             85..>2800
                     /NRPS_PKS="Domain: PCP (0-65). E-value: 8.9e-21. Score:
                     65.9. Matches aSDomain: nrpspksdomains_C6351_RS32010_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (89-390). E-value:
                     3.6e-106. Score: 346.5. Matches aSDomain:
                     nrpspksdomains_C6351_RS32010_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (555-904). E-value: 2.5e-93.
                     Score: 304.8. Matches aSDomain:
                     nrpspksdomains_C6351_RS32010_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /locus_tag="C6351_RS32010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6351_32015"
                     /product="amino acid adenylation domain-containing protein"
                     /protein_id="WP_261982300.1"
                     /sec_met_domain="PP-binding (E-value: 6.7e-18, bitscore:
                     51.3, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 1.9e-73, bitscore:
                     233.2, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 2.9e-90, bitscore:
                     288.8, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVEIWSEVLGIDSSAIGINDNFFELGGHSLLATKITSRLGVDFSI
                     LVQIRDIFEYPTIKHFSKRLTFLLGKTGEIDVFTPLQSVKREQYEPLSFAQQRLWIMDK
                     IVTNSALYNVPIAWRLKGKWNIDMLKKGYNAIIHRHEILRTVFHEVEGKPVQIVQQDIT
                     VPLSVIDLRYLSLSPEDKTSKIENISKIEAKKPFDLSQGPLLRTHLIIMDNKELVLLCT
                     IHHIIFDRWSLDTFINEWMSFYQAFLENETEKLPPLPVQYIDFTKWQKSWLTEDIIQKQ
                     IAYWGKELRGLLPILELPLDYPRPIIQSYNGDTYQIVLPNKLLEKIKVFNSKEGVTLFA
                     TLLASYQGFLSRYTNQKDILVGSPIANRNYPGVEDLIGFFANTVVYRADCSNNITFKEL
                     VQQIKKKMLDAQENQDVPFEKVIEAIQPERDMSYSPLFQTMFVFHNQLKELPKLLDHSV
                     EKIPRHMNGAKFDITIAMEEIPTGLQVDFEYNTDLFKVSTIKRMAQSFEIWLDEILQFP
                     EEPIENLRILTKNEEKQLLHEWTNIEGNYQKDVVIQELFEQQVIQNPDSVALVYKDQQL
                     TYKELNEKVNQLAFYLQKRNIGPESMVGVYIERSLEMIVSILGIIKAGGAYVPLDPAYP
                     TKRLEYILKDANIQVLLTQSHLTQWIPKEIDCIDIKEHEMNISREKNINPTIEVKPDNL
                     AYVIYTSGSTGNPKGVLYEQKGLCNFINASINFTKLNTGSKVVQFSSIAFDVSLYDIFA
                     TLVSGGTLYVCGQQDIMPVEPLTQFLLEKKITHAFLPPTVLNLLDESKFEELQTVISAG
                     SVCSEQVAKRWVKNHLFINAYGPTEASVATIDIYSGKGTPPIGRSIPNVEVYVLNEAKK
                     LVPIGTVGELYIGGIALARGYLNQPELTKASFIP"
     aSDomain        352..1254
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_C6351_RS32010_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="3.60E-106"
                     /label="C6351_RS32010_Condensation_LCL.1"
                     /locus_tag="C6351_RS32010"
                     /protein_end="390"
                     /protein_start="89"
                     /score="346.5"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIIFDR"
                     /tool="antismash"
                     /translation="EPLSFAQQRLWIMDKIVTNSALYNVPIAWRLKGKWNIDMLKKGYN
                     AIIHRHEILRTVFHEVEGKPVQIVQQDITVPLSVIDLRYLSLSPEDKTSKIENISKIEA
                     KKPFDLSQGPLLRTHLIIMDNKELVLLCTIHHIIFDRWSLDTFINEWMSFYQAFLENET
                     EKLPPLPVQYIDFTKWQKSWLTEDIIQKQIAYWGKELRGLLPILELPLDYPRPIIQSYN
                     GDTYQIVLPNKLLEKIKVFNSKEGVTLFATLLASYQGFLSRYTNQKDILVGSPIANRNY
                     PGVEDLIGFFANTVVYRADC"
     aSModule        352..2796
                     /domains="nrpspksdomains_C6351_RS32010_Condensation_LCL.1"
                     /domains="nrpspksdomains_C6351_RS32010_AMP-binding.1"
                     /incomplete
                     /locus_tags="C6351_RS32010"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       412..528
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C6351_RS32010_0001"
                     /evalue="2.60E-14"
                     /label="C2_LCL_024-062"
                     /locus_tag="C6351_RS32010"
                     /protein_end="148"
                     /protein_start="109"
                     /score="45.6"
                     /tool="antismash"
                     /translation="ALYNVPIAWRLKGKWNIDMLKKGYNAIIHRHEILRTVFH"
     CDS_motif       751..816
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C6351_RS32010_0002"
                     /evalue="3.30E-08"
                     /label="C3_LCL_132-143"
                     /locus_tag="C6351_RS32010"
                     /protein_end="244"
                     /protein_start="222"
                     /score="26.1"
                     /tool="antismash"
                     /translation="IHHIIFDRWSLDTFINEWMSFY"
     CDS_motif       865..903
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C6351_RS32010_0003"
                     /evalue="2.30E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="C6351_RS32010"
                     /protein_end="273"
                     /protein_start="260"
                     /score="13.8"
                     /tool="antismash"
                     /translation="QYIDFTKWQKSWL"
     CDS_motif       1156..1245
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C6351_RS32010_0004"
                     /evalue="1.40E-15"
                     /label="C5_LCL_267-296"
                     /locus_tag="C6351_RS32010"
                     /protein_end="387"
                     /protein_start="357"
                     /score="49.6"
                     /tool="antismash"
                     /translation="DILVGSPIANRNYPGVEDLIGFFANTVVYR"
     CDS_motif       1297..1413
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C6351_RS32010_0005"
                     /evalue="2.20E-22"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="C6351_RS32010"
                     /protein_end="443"
                     /protein_start="404"
                     /score="71.5"
                     /tool="antismash"
                     /translation="KKMLDAQENQDVPFEKVIEAIQPERDMSYSPLFQTMFVF"
     aSDomain        1750..2796
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_C6351_RS32010_AMP-binding.1"
                     /evalue="2.50E-93"
                     /label="C6351_RS32010_AMP-binding.1"
                     /locus_tag="C6351_RS32010"
                     /protein_end="904"
                     /protein_start="555"
                     /score="304.8"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="FEQQVIQNPDSVALVYKDQQLTYKELNEKVNQLAFYLQKRNIGPE
                     SMVGVYIERSLEMIVSILGIIKAGGAYVPLDPAYPTKRLEYILKDANIQVLLTQSHLTQ
                     WIPKEIDCIDIKEHEMNISREKNINPTIEVKPDNLAYVIYTSGSTGNPKGVLYEQKGLC
                     NFINASINFTKLNTGSKVVQFSSIAFDVSLYDIFATLVSGGTLYVCGQQDIMPVEPLTQ
                     FLLEKKITHAFLPPTVLNLLDESKFEELQTVISAGSVCSEQVAKRWVKNHLFINAYGPT
                     EASVATIDIYSGKGTPPIGRSIPNVEVYVLNEAKKLVPIGTVGELYIGGIALARGYLNQ
                     PELTKASFI"
     CDS_motif       1936..1977
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C6351_RS32010_0006"
                     /evalue="1.00E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="C6351_RS32010"
                     /protein_end="631"
                     /protein_start="617"
                     /score="18.6"
                     /tool="antismash"
                     /translation="LGIIKAGGAYVPLD"
     CDS_motif       2167..2223
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C6351_RS32010_0007"
                     /evalue="5.10E-09"
                     /label="NRPS-A_a3"
                     /locus_tag="C6351_RS32010"
                     /protein_end="713"
                     /protein_start="694"
                     /score="28.2"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGNPKGVLYE"
     CDS_motif       2704..2793
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C6351_RS32010_0008"
                     /evalue="8.80E-17"
                     /label="NRPS-A_a6"
                     /locus_tag="C6351_RS32010"
                     /protein_end="903"
                     /protein_start="873"
                     /score="53.2"
                     /tool="antismash"
                     /translation="PIGTVGELYIGGIALARGYLNQPELTKASF"
ORIGIN
        1 gataaagatg ctttacctgc atgggcctct attatacaaa cgaacgaggg atatatagca
       61 cctcggaata gagtagagca aaaaatggta gaaatttggt cagaggtatt agggattgac
      121 tcctctgcca ttggaataaa cgataatttt tttgagttag gtggacactc tctcctagca
      181 actaaaataa cttcacgttt aggtgttgat ttttcaattc tagtacaaat acgtgatata
      241 tttgaatacc ctaccattaa acatttctca aaacgtttaa catttttatt gggaaaaacg
      301 ggggagatcg atgtttttac accattacaa tcagttaaac gtgaacaata cgaaccatta
      361 tctttcgctc aacagcgttt atggataatg gataaaattg taacaaatag tgcgctttac
      421 aatgtcccga ttgcatggcg tttaaaagga aaatggaaca ttgatatgtt aaaaaaagga
      481 tataatgcaa taatacatcg ccatgaaatt ctacggacag tatttcatga agttgaagga
      541 aaaccagtgc aaattgttca acaagatata acagttccct tatcagtaat tgatttgcgt
      601 tatctttctc tttctcctga agataaaaca tctaaaatag aaaatatatc aaaaatagaa
      661 gctaaaaagc catttgattt aagccaagga cctttattac gtactcattt aataataatg
      721 gacaataaag agttagtact tctttgcacc atccatcata ttatttttga tcgctggtca
      781 ttagatacct ttatcaatga atggatgagt ttttatcagg catttttaga aaacgaaact
      841 gaaaagcttc cccctttacc tgttcagtat attgatttta caaaatggca aaaaagttgg
      901 ttaacagaag acattataca aaaacaaata gcatattggg ggaaagaatt acgcggttta
      961 cttcctatat tggaactccc attggattac cctcgtccaa taatacaaag ctataatgga
     1021 gatacgtatc aaattgtcct acccaataaa ttgttagaaa aaataaaagt attcaattct
     1081 aaggaaggcg ttacattatt cgccacatta ttagcaagtt atcaagggtt tctttctcgt
     1141 tataccaatc aaaaagatat tttagtaggc agcccaattg caaataggaa ttatccagga
     1201 gttgaagatc ttattggatt ctttgcaaat actgtagtct atcgtgcaga ttgctcaaat
     1261 aatataacat ttaaagaatt ggtccaacaa ataaagaaaa aaatgttaga tgcacaagaa
     1321 aaccaggacg ttccttttga aaaagttata gaagctatac aaccagaacg tgatatgagt
     1381 tattctccat tatttcagac aatgtttgtt tttcacaatc aattaaaaga gttaccaaaa
     1441 ttattagatc atagcgtaga aaaaatccct agacatatga atggtgcaaa gtttgatata
     1501 actatagcaa tggaagagat accaacagga ttacaagtgg attttgaata taatacagac
     1561 ttatttaagg tttctacaat aaaaagaatg gctcaatctt ttgaaatttg gctagatgaa
     1621 attttacaat ttccggagga acctattgag aatctaagaa tactcactaa gaacgaagaa
     1681 aagcaattat tacatgaatg gactaacata gagggcaatt atcaaaaaga tgtagtgatt
     1741 caagagttat ttgaacagca agtaattcag aatccggatt ctgttgcatt ggtatataaa
     1801 gatcaacagt taacttataa ggaattaaat gaaaaagtaa atcaattagc tttttatcta
     1861 caaaaacgaa acataggccc tgaaagtatg gtaggggttt atatagaacg ttctttagag
     1921 atgattgtaa gtatattagg aataataaaa gctggtggcg catatgtccc actagatccc
     1981 gcatatccaa caaagaggct tgaatatatc ttgaaagatg ccaacataca agtattgctg
     2041 acacaaagtc atctaacaca gtggatacct aaagaaatag attgtattga tataaaagaa
     2101 catgaaatga atatttcgag agaaaaaaat ataaatccta caattgaagt taaaccagat
     2161 aatttggcat atgtaattta tacatctgga tccacaggaa atccaaaggg tgtcctttat
     2221 gaacaaaaag gtctatgtaa ttttattaat gccagcatta attttacaaa attaaatacg
     2281 ggtagtaagg tagttcagtt ttcttcaatt gcatttgatg tttcactata tgatatcttc
     2341 gcaactctgg tgtctggtgg aactttatat gtatgtggtc aacaagatat tatgccagtc
     2401 gagccactta cccagttttt gctagaaaaa aaaattacgc atgcattttt accacctacg
     2461 gtactaaatc tgttagatga atctaaattt gaggaattac aaactgttat ttctgcgggt
     2521 tcagtctgta gtgaacaagt ggcaaaacga tgggtgaaaa atcacctctt tataaatgca
     2581 tatggtccaa cagaagcatc ggttgctact atagacattt atagcggcaa agggacacca
     2641 cctattggtc gatctattcc caatgtagag gtgtatgtat taaatgaagc taaaaaactt
     2701 gtccctattg gaacagtagg tgaactttat ataggaggta tcgcattagc acgaggatat
     2761 ttgaatcaac ctgaattaac aaaggcctca tttatcccac
//
