LOCUS       NZ_PVRL01000013        10312 bp    DNA     linear   CON 21-MAY-2024
DEFINITION  Bacillus thuringiensis strain NMTD81
            NODE_13_length_159018_cov_11.0582, whole genome shotgun sequence.
ACCESSION   NZ_PVRL01000013
VERSION     NZ_PVRL01000013.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 159018)
  AUTHORS   Borriss,R., Lasch,P., Wu,L., W,H. and Gao,X.
  TITLE     Genotypic and phenotypic analysis of antagonistic Bacillus spp.
            isolated from rhizosphere soil of plants in Tibet
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 159018)
  AUTHORS   Borriss,R., Lasch,P., Wu,L., W,H. and Gao,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2018) UG, NordReet, Marienstr. 27a, Greifswald
            17489, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: A5 v. A5-2017
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 14.5x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002998815.1-RS_2024_05_21
            Annotation Date                   :: 05/21/2024 01:45:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,177
            CDSs (total)                      :: 6,068
            Genes (coding)                    :: 5,840
            CDSs (with protein)               :: 5,840
            Genes (RNA)                       :: 109
            rRNAs                             :: 11, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 1 (5S, 23S)
            partial rRNAs                     :: 6, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 228
            CDSs (without protein)            :: 228
            Pseudo Genes (ambiguous residues) :: 0 of 228
            Pseudo Genes (frameshifted)       :: 100 of 228
            Pseudo Genes (incomplete)         :: 142 of 228
            Pseudo Genes (internal stop)      :: 67 of 228
            Pseudo Genes (multiple problems)  :: 63 of 228
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:59:29
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 124100
            Orig. end                         :: 134412
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            PVRL01000013.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10312
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10312
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10312
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5312](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5312
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    433..447
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 17.35"
                     /tool="antismash"
     gene            523..1971
                     /locus_tag="C6351_RS24030"
                     /old_locus_tag="C6351_24040"
     CDS             523..1971
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (145-469). E-value:
                     1.7e-30. Score: 98.2. Matches aSDomain:
                     nrpspksdomains_C6351_RS24030_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019: aminotransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016108507.1"
                     /locus_tag="C6351_RS24030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6351_24040"
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="WP_000471637.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 4.9e-30, bitscore:
                     98.1, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFKDFKVVKDRPVYIQLKDYLKKMIMKGHLLGNQKIPSTRELSEL
                     LSVSRNTVLAAYADLEQEGLIYAVKGKGNFVAKVDISNTSSVEIDWKNKLNTVTALADE
                     LDLMKHGVRWEKGMIVFNSIAPDEKLFDVENFKRAFLTRMSIEGDIVLNYGYAKGYKPL
                     MNYLLHYMEMKGVDISNKDILITNGFTEGLDIVLSSLSKKSGRVICENPTHHAALKLFR
                     LHGLEVHGIDMNEDGIDTNEVEKSLREKDFDFAYLIPSYHNPTGIVTSSEKRTELMRLF
                     SKYKVPIIEDGFNEELRYSGSHLAPLLTFAGAGNNVIYISSFSKVLFPGLRVGWIIADK
                     ELIHYLESVKRARTIHTSTLDQAVLFQYLHEGYFEKYLKKARSVYKKKYELAVGACNQF
                     IPFKRMTGDGGLHLFIELEEHMNARTLLQKCYEKGVTFSPGDVFYSDGEGANTFRLGFS
                     RLKEEEIVRGIKIIGDTLKNEIWS"
     aSDomain        958..1929
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_C6351_RS24030_Aminotran_1_2.1"
                     /evalue="1.70E-30"
                     /label="C6351_RS24030_Aminotran_1_2.1"
                     /locus_tag="C6351_RS24030"
                     /protein_end="469"
                     /protein_start="145"
                     /score="98.2"
                     /tool="antismash"
                     /translation="IEGDIVLNYGYAKGYKPLMNYLLHYMEMKGVDISNKDILITNGFT
                     EGLDIVLSSLSKKSGRVICENPTHHAALKLFRLHGLEVHGIDMNEDGIDTNEVEKSLRE
                     KDFDFAYLIPSYHNPTGIVTSSEKRTELMRLFSKYKVPIIEDGFNEELRYSGSHLAPLL
                     TFAGAGNNVIYISSFSKVLFPGLRVGWIIADKELIHYLESVKRARTIHTSTLDQAVLFQ
                     YLHEGYFEKYLKKARSVYKKKYELAVGACNQFIPFKRMTGDGGLHLFIELEEHMNARTL
                     LQKCYEKGVTFSPGDVFYSDGEGANTFRLGFSRLKEEEIVRGI"
     gene            1976..2890
                     /locus_tag="C6351_RS24035"
                     /old_locus_tag="C6351_24045"
     CDS             1976..2890
                     /EC_number="6.3.2.4"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RimK"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002038752.1"
                     /locus_tag="C6351_RS24035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6351_24045"
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="WP_002082718.1"
                     /sec_met_domain="Dala_Dala_lig_N (E-value: 2.4e-23,
                     bitscore: 76.0, seeds: 508, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp_3 (E-value: 3e-15, bitscore:
                     50.0, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="RimK (E-value: 3.2e-10, bitscore: 33.2,
                     seeds: 4, tool: rule-based-clusters)"
                     /sec_met_domain="Dala_Dala_lig_C (E-value: 1e-54, bitscore:
                     178.4, seeds: 12, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp (E-value: 3.2e-07, bitscore:
                     23.4, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIGVIMGGVSSEKQVSIMTGNEMIAHLDKNKYEIVPITLNEKMD
                     LIEKAKDIDFALLALHGKYGEDGTVQGTLESLGIPYSGSNMLSSSICMDKNISKKILRY
                     EGVETPDWIELTKMEDLKLDELDKLGFPLVVKPNSGGSSVGVKIVYNKNELISMLETVF
                     EWDSEVVIEKYIKGDEITCSILDGKQLPIVSIRHAAEFFDYNAKYDDTSTVEEVIELPA
                     EINERVNKASLACYKALKCSVYARVDMMVKDGIPYVMEINTLPGMTQSSLLPKSAEAAG
                     ISYSKLLDMIIETSLKVRKEEGF"
     misc_feature    complement(2913..2925)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.27"
                     /tool="antismash"
     regulatory      2964..3069
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /regulatory_class="riboswitch"
     gene            3197..3886
                     /gene="tenA"
                     /locus_tag="C6351_RS24040"
                     /old_locus_tag="C6351_24050"
     CDS             3197..3886
                     /EC_number="3.5.99.2"
                     /GO_function="GO:0050334 - thiaminase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="tenA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144490.1"
                     /locus_tag="C6351_RS24040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6351_24050"
                     /product="thiaminase II"
                     /protein_id="WP_000144509.1"
                     /transl_table=11
                     /translation="MTFSQSLRKEVDSIWEASFNHPFVKKLGEGTLDLASFRYYVLQDS
                     YYLSHFARVQTLGAAKALELETTARMAHHAQNTYEAELSLHENFAKKLGITKEEKDNFI
                     PAPTAYAYTSHMYRAAYEGHLGDIIAAILPCYWLYYEIGERLKECQPEEPIYNEWISAY
                     GSDWFRTLVEEQITRLDTIAEKVTEADRKRMKQHFIISSQYEYSFWEMAYTLEKWPVDT
                     DVKDVIG"
     gene            4284..4547
                     /locus_tag="C6351_RS24050"
                     /old_locus_tag="C6351_24060"
     CDS             4284..4547
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002101034.1"
                     /locus_tag="C6351_RS24050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6351_24060"
                     /product="DUF3937 domain-containing protein"
                     /protein_id="WP_002082716.1"
                     /transl_table=11
                     /translation="MNFERCYMFTNKKLIRFGLSLFVFLGIINFTISYFQTYLETAADI
                     KWVIPEIWKTILLDVPQGILVLLGAIALYDFTKEASQKDASI"
     gene            5001..5312
                     /locus_tag="C6351_RS24055"
                     /old_locus_tag="C6351_24065"
     CDS             5001..5312
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071363.1"
                     /locus_tag="C6351_RS24055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6351_24065"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_142399144.1"
                     /sec_met_domain="TIGR03601 (E-value: 1.6e-45, bitscore:
                     146.8, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQFQQEIQALSLNDYRSGNIVYWDQQNQYPYYYIEDAARRCGGC
                     GRCGGCGGGRCGGFRCGGFRCIGCFGCFGCGGCGGCSNCFDGFSGTTDTTGTIITYEY"
     gene            <5462..5596
                     /locus_tag="C6351_RS24060"
                     /old_locus_tag="C6351_24070"
                     /pseudo=""
     CDS             <5462..5596
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016077325.1"
                     /locus_tag="C6351_RS24060"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="C6351_24070"
                     /product="site-specific integrase"
                     /pseudo=""
                     /transl_table=11
                     /translation="YMYVPTKNIVIINGYIQSFK"
     misc_feature    complement(5756..5774)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: medium, score: 24.87"
                     /tool="antismash"
     gene            5806..6291
                     /locus_tag="C6351_RS24065"
                     /old_locus_tag="C6351_24075"
     CDS             5806..6291
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016124614.1"
                     /locus_tag="C6351_RS24065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6351_24075"
                     /product="hypothetical protein"
                     /protein_id="WP_002082715.1"
                     /transl_table=11
                     /translation="MNMVELKSIFHSYKVKRKIAKDLYGKRDELTLLLNEFNNMKCTVT
                     SEKKKNNILSRLQLIYQNIKLDKQYPLSVSFDSKLLERLEKESLHTIEDGVTCLHLMLD
                     MNYEKIKQYGSSTSRSFVPLSQSSICLADCICLTGFIVGLLGVISFGGFMLSICSTT"
     misc_feature    complement(6538..6551)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 20.22"
                     /tool="antismash"
     misc_feature    6593..6606
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.3"
                     /tool="antismash"
     gene            6598..8985
                     /locus_tag="C6351_RS24070"
                     /old_locus_tag="C6351_24080"
     CDS             6598..8985
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736192.1"
                     /locus_tag="C6351_RS24070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6351_24080"
                     /product="RNaseH domain-containing protein"
                     /protein_id="WP_081143677.1"
                     /transl_table=11
                     /translation="MKKLRLLTFENIVEPLLNEKVSFIYFPIEWLDIVEIHYKTFLLTS
                     KLKRLNERLYDMFSDILFIQHNPYVLNENTPWIVSKEPIKQEQLNYIFQSWYEIIHDWK
                     PNKLVEPPKYEWQSDLISNLPVLHDNETYSKWVPALISHIFCERPIYLENTNAEEIYFS
                     PLRSQNICEAMSGPIKDEKTQDFFSYVYRFECITRGGENAPLLNISIGIRRFYQEYNHP
                     DIPLLFRRKRGMILISTPEFASNNKKIRFVKLKMQQATTGMKWIKIFRNLKDDFQIGGD
                     LDLDHILQYPKDYIVGENKRVLFPYNERIYKVQGTKIEPGIKVKEREYLFKEFQRKFSY
                     FTILPECKKVATNNKNELFPLFAPKGLETLTLEVWSENIALEIEQALLDSEIVLTKIGE
                     NSYVLNTDFPVLLKIVRYNIEKVLQNPYKMQYCQKYKTNLVDDVTKAVYATAGERSDAT
                     LALIAMKNCDGREKIDPKQIIREGFARTNRISTFINLFIGQSVSRKTIVNSVFSLLEQK
                     GFLKRSWNKINLPCTYVNLSIERISKFDFLPILSKIKGKEILYKLYGNTEWQKIDYVLL
                     NINKHNAFLSQPSKRNDMGALFKQYVFETLMEIVQYAKEQNEQVYFIVDANLRKYWIKE
                     LQNKEINTDILPDIVPEVLKVPNLNIIRINTGFDVPSYGVIECDDALDRIGLYADQKGM
                     YYSTGEYSLNCSEVFQRYIIEILPLGVKPVERDYIAKMIHYMCCNSSMLSKKNVHMTYS
                     VHMEKVIKSYITDIDAREFKEFDDELDVDVVKVEKKDSIILL"
     gene            9193..9960
                     /locus_tag="C6351_RS24075"
                     /old_locus_tag="C6351_24085"
     CDS             9193..9960
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017560251.1"
                     /locus_tag="C6351_RS24075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6351_24085"
                     /product="DUF3959 family protein"
                     /protein_id="WP_074790193.1"
                     /transl_table=11
                     /translation="MKKLDVLLMLLSVLFPIAGLTKQIPLEQSVYIGGLLFFTSFGSYF
                     AKKMYSRICSWIAYAPFITLLLVIWHQDITTSSIIVNAKIAACVALIPCLFRFRTYGLT
                     LGLFSLWAALLWDIKEVQSLVILERMTSLMTSHYVYILLLIGGLVLGGLLAMFIHRKEK
                     DNNKENINLLGPKKKRKRLSFKIRLPRLPKLKMKLFKFGGKESKSKIPEKIHEHKYEEP
                     VATYKMTEKIDPYKENEVQGQTRMERRRNRFNA"
ORIGIN
        1 gaaagaagcg caaaataaag gaattgctga gacaaaccct atattagata taagtggttc
       61 agatagtgcg tgtaaattgt tacttttgac aaacagctta atggaaacag agaaaacact
      121 tactgatata catataaaag gaatcgagca cgttacaaaa cagcaaatac aaaatgctaa
      181 ggaacaacat aaaattatta aattaatagc atcaatatat aaggatgagg gtggcaatgt
      241 gaatttaaat gttgaaccgt gcgaaataga aaaagatcat ccgttagcaa aagtaaatgg
      301 gaccgaaaaa ggaattacgt tctttacaga tacgatggga caagttacta caattggtgg
      361 ggcttctaat ccacgaggcg ctgcagctgc agctttaaaa gatgtaatta acttatatcg
      421 aaaagacttg taagaccgta ccgcccttta taaaaaatac atatgtcgtg gaccttttgt
      481 gaatgaaata aatatgcaaa aatgattgca gggggtaagg aattgtttaa ggattttaaa
      541 gttgttaaag atcgtcccgt ttatattcaa ttgaaagatt atttgaagaa gatgattatg
      601 aaagggcact tgctcgggaa tcaaaaaatc ccatcaacaa gggaactaag tgaattacta
      661 agtgtgagta gaaacactgt acttgctgct tatgcagatt tagagcaaga aggactcatt
      721 tatgcagtta aaggaaaagg gaactttgtt gcgaaggtag atatatctaa cacttcgtct
      781 gttgaaatag attggaaaaa taaacttaat acagtgaccg cattagcgga tgaattagat
      841 ttaatgaaac atggtgttcg ttgggaaaag ggaatgattg tttttaatag tatagccccg
      901 gatgagaagc tatttgatgt tgaaaacttc aaaagagctt ttcttactcg tatgtccatt
      961 gaaggggaca tcgtattgaa ctacggatat gcaaagggat ataaaccgtt aatgaattat
     1021 ttacttcatt acatggaaat gaaaggtgta gacatttcga ataaagatat tctaattaca
     1081 aatggattta cagaaggatt ggatattgta ctttcttctt tatcaaaaaa atccggtcgt
     1141 gtcatttgtg agaatccaac tcaccacgct gcactaaagc tgttccgttt acatggactt
     1201 gaagttcatg ggattgatat gaatgaggat ggtattgata cgaatgaagt tgaaaaaagc
     1261 ttacgtgaaa aagattttga ttttgcttat ttaattcctt cttatcataa tccaaccggt
     1321 attgttacga gttcagagaa aagaacggaa ttgatgaggt tattctcgaa atataaggtt
     1381 cctattatag aggacggatt taatgaagaa ttacgttatt caggttcaca tttagctccc
     1441 ttattaactt ttgctggagc tggcaataat gtgatttata ttagtagctt ttcaaaggta
     1501 cttttccctg gtttacgtgt aggttggatt attgcagata aagagctgat tcattattta
     1561 gaaagcgtaa aaagagcaag aacaatccac acatctacgt tagatcaagc ggttctattt
     1621 caatatttac atgagggata ttttgagaaa tatttaaaaa aggcaagatc ggtttataag
     1681 aaaaaatatg agttagctgt cggcgcgtgt aatcagttca ttcctttcaa aagaatgact
     1741 ggagatggtg gacttcattt atttatagag ctagaagagc atatgaatgc ccgcacactt
     1801 ttacaaaagt gttatgagaa aggcgttacg ttttcacctg gagatgtttt ttactctgat
     1861 ggagaagggg cgaacacttt ccgattaggg ttttcgcgct tgaaagaaga agaaatagtt
     1921 cggggaatta aaataattgg tgatacatta aaaaatgaaa tttggagttg agaaaatgaa
     1981 aatcggtgtt attatgggcg gggtatcgtc tgaaaaacaa gtttcaatta tgacagggaa
     2041 tgaaatgatt gctcatttag ataaaaataa gtatgaaata gtcccaatta cattaaatga
     2101 aaaaatggat ttaattgaaa aagcgaaaga cattgacttt gcgttgctcg cattacacgg
     2161 gaaatatgga gaagatggga cagttcaagg aacactggag agtttaggga ttccttatag
     2221 cggaagtaat atgttatcta gtagtatatg tatggataag aatatttcga aaaagatttt
     2281 acgttatgaa ggtgtagaaa caccagattg gattgaactt acaaaaatgg aggatctaaa
     2341 gcttgatgag ttagataagt taggatttcc attagtggta aaaccaaact ctggcggatc
     2401 gagtgtcggg gtgaaaatcg tttacaataa aaatgaattg atctcaatgc tagaaactgt
     2461 atttgaatgg gattctgaag tagtaattga gaagtatata aaaggtgatg aaattacatg
     2521 ttccattctg gatggaaaac agttacctat agtttcaatt cgacatgcag ccgagttctt
     2581 tgactacaat gcaaaatatg atgatactag tacagttgag gaagttatcg aacttccagc
     2641 ggaaattaat gaacgtgtaa ataaagcgtc actggcttgt tataaagcat taaaatgtag
     2701 tgtttatgca agagttgata tgatggtgaa ggatggaatt ccatatgtaa tggaaattaa
     2761 tacattacca gggatgacac aatcaagttt attgccaaaa agtgcagaag cagcgggaat
     2821 tagttatagc aaactattag atatgattat tgaaacatca ttaaaagtta gaaaagaaga
     2881 agggttttaa ggggattata taactaattc ttttgacagt ttcacgaagt atgctatgat
     2941 gaatacatta taaaaacaaa atatttatcc actagggggg ccttttatag gctgagatca
     3001 aatgtgattt tgagactctt agtacctgat ctggttaatg ccagcgtagg gaagtgggaa
     3061 agacgttgct atttaatgag taaataaata ctgtcaattt cactttcttt acgcctgtaa
     3121 agaaagtttt tttattttac agctttaata attgtggata aatacttata ttttatacat
     3181 tattggaggg atttaaatga ctttttcaca atcattacgt aaagaagtag attcaatttg
     3241 ggaagcgagt tttaaccatc cttttgtaaa aaaacttggt gaaggaacgt tagatttagc
     3301 tagttttcgc tattatgtgc ttcaagattc atattatttg agtcattttg ctagagtaca
     3361 aactttagga gctgcaaaag ctcttgaatt agagacgaca gctcgtatgg cacatcatgc
     3421 tcaaaataca tatgaggccg agttatcttt acatgagaat tttgctaaaa agctagggat
     3481 aacaaaagaa gaaaaagata attttattcc tgcgccaact gcatatgcgt atacttcaca
     3541 tatgtatcgt gctgcgtatg aagggcattt aggagatatc attgcagcaa ttttaccttg
     3601 ctattggttg tattacgaaa ttggggaacg tttaaaagaa tgtcagccgg aagagccaat
     3661 ttataacgaa tggatttctg cttatggatc tgattggttc cgtacgctag ttgaagaaca
     3721 aattacacgg ttagatacta ttgctgaaaa agtaacagag gcagaccgca agcgtatgaa
     3781 acagcatttt attattagca gtcaatatga atattcattt tgggaaatgg cttatacatt
     3841 agaaaagtgg ccagtggata cagacgtaaa agacgtaatt ggataaagaa tagaagtaat
     3901 gttagagata ggtatgatcc atacctatct ctatattttt tcttgtcaaa tttgcgtaaa
     3961 gtcattagtg ctacggatat ggtgaaatag aatatcgata atttagctca tttaattgtc
     4021 tacgcattaa ctggataaac ttctgtcaca tttattttac aattaaagaa cgtaaatgtt
     4081 ggtggtatga agttaaataa gaaaagaaac ccgagctgtt agctgtaacg gagttgtttt
     4141 ttctttgcag gaatttctaa gttaacatga agtgctgttc atacaatatg caaatttgca
     4201 tattgtattt ttttgagttc tttgtgtgtt aaaatcaaat atagagatat ggaaatataa
     4261 agaagcaaaa gcaggaatat ccaatgaatt ttgagaggtg ttatatgttt acaaataaga
     4321 aattaattcg atttggttta tcgttgtttg tatttttagg gataattaat tttacaatca
     4381 gctatttcca aacatatctt gaaacagcag cagatattaa atgggtaatt ccagaaattt
     4441 ggaaaactat tttactagat gttcctcaag gtatacttgt tcttttaggc gcaattgcat
     4501 tatatgattt cacaaaagag gcatcacaaa aagacgcatc aatctaagtg tgtctttttt
     4561 caattagaaa tttcatagag agctttgctg gagaaataga ataaaaagcc ctaattaggg
     4621 cttttttctt tataggaatt tcttaaccgt catagaaacc ggttacttag aggtgttata
     4681 gcaaaggcat ttacagtaca agaaaatcta agtgatatat ggaaatatat aggatttgtc
     4741 cgcgtgatta ttgagaaatt aattgagctt agtaagtatg aagatatgac gctagaattt
     4801 atattttata atttctattt ataaaaatga gcccatagta aaaataggta tatgaacaag
     4861 gtgcatcatc tagtgataag tcatactttg ttggtgtacg tattgaactt agtttatagc
     4921 gctatctcta cttagatgta acgaacagaa atatagggaa gcatcaatga ggttgtattt
     4981 tataaacgga ggttatgaaa atgaatcagt ttcaacaaga aatacaagcg ttaagcctta
     5041 atgattaccg atctggaaat attgtctatt gggaccagca aaatcaatat ccatattact
     5101 atattgaaga tgctgctcgt cgctgtgggg gttgtggtcg ttgtggcggt tgtggcggag
     5161 gtcgttgtgg cggattccgt tgtggtggat tccgttgtat tggttgcttc ggttgttttg
     5221 gttgcggagg ttgtggcggt tgctctaact gttttgatgg ttttagtggt actactgata
     5281 ctactggtac tattataacg tatgaatatt gattgcattt atcctgctat ttgccgggca
     5341 gtaagaccct aaaaaaattc agcgagagca aagagaagtt aggtggccgg ctgcccataa
     5401 aagcccgatg gatgagggct aattaaagtt tcaccttatc aattaaaaaa cattagtttt
     5461 atatatgtac gtgccgacaa agaatatagt cattattaat ggatatattc aatcattcaa
     5521 gtgaaagagt aacaataaga tatataggtg taatcaagat gtgatggata aagcaatgac
     5581 tagatttaaa atctaatcat tgtttttttc ttatggtttt atcattaaat atctgtctag
     5641 tttgggcatt gcttccttct agctaatgat tttattacaa ccgtgtaagg ttaatgtaat
     5701 gttaatcgat agtaaaataa gttgcttgag tgtattttaa ataagattaa aaagtactgt
     5761 ttatctataa aataattggg taaataatat gtaattagga agtgtatgaa tatggttgaa
     5821 ctaaaatcta ttttccattc gtataaagta aagagaaaaa tagctaaaga tttatacgga
     5881 aaaagagatg aattgacact gttattaaat gaatttaata atatgaaatg tacagtaaca
     5941 tctgaaaaga agaaaaataa tatattatct cgtttgcaac taatttatca aaatataaaa
     6001 ttagataagc agtatcctct atcagtttct tttgatagta aattattgga gcgattagaa
     6061 aaagaatctc tacatactat tgaggatggt gtaacatgtc tacatttaat gttagatatg
     6121 aattatgaaa aaataaaaca atatgggtcg agtacaagta ggtcattcgt tccattatcg
     6181 cagtcttcta tttgtcttgc tgattgtatt tgtttaacag gatttatagt aggtttacta
     6241 ggagtaattt catttggagg gttcatgtta tctatatgtt caactacata acgcttgata
     6301 tttgtaaaaa gagcaccgat ttaaggtgtt cttttttggt ttttctgtaa agttattgta
     6361 aatgataagt acaatatatt atatatatta tataatttaa atgtattaaa ttacgaatac
     6421 taagaggtgt tgtagataat aattactttt aatttttaaa agagtcggtt tgataatgtt
     6481 ttatgtagat agtttttact tttcttttaa atactttgtt taaatggatt agattaagta
     6541 acttttgttc tagctttttg ataatcaaga caactgaaga aaggaaagaa tcattttatg
     6601 aaaaaattaa ggttgttaac atttgaaaat atagtagaac ctcttttaaa tgaaaaggta
     6661 tcatttatat actttcctat tgaatggctg gacatcgtag agatacatta taagacgttt
     6721 ttattaacga gtaagttgaa acgtttgaat gaaagactgt atgatatgtt ttctgatata
     6781 ttgtttattc agcataatcc gtacgtatta aatgaaaata caccatggat tgtatcaaaa
     6841 gaacctatta aacaagagca gctcaattat atttttcaaa gttggtatga gattattcat
     6901 gattggaaac caaataaatt agtagagcca ccaaaatatg aatggcaatc cgatttgatt
     6961 tctaatttgc cagtactaca tgataatgaa acgtattcta agtgggtgcc cgctttaatc
     7021 tcacatattt tttgtgagcg tcctatatat ttagaaaata caaatgcaga agaaatctat
     7081 ttttctcctc ttagatcaca aaatatttgt gaggcgatgt cagggccaat aaaagatgaa
     7141 aaaacacaag attttttctc ctatgtatat cgattcgaat gcataacccg tggtggtgag
     7201 aacgctccat tattaaatat ttcaataggg attcggagat tttatcaaga atataatcat
     7261 ccagatattc ctttactttt cagacgaaaa cgagggatga tattgatttc taccccagag
     7321 tttgcatcaa ataataaaaa aatacggttt gtaaaactaa aaatgcaaca agctacaacc
     7381 ggcatgaaat ggattaagat atttagaaat ttaaaagatg attttcaaat aggtggagat
     7441 ttggatttag accatattct ccaatatccg aaagattata ttgtcgggga aaataaaagg
     7501 gtgttatttc catacaatga aagaatatat aaagtccaag gtactaaaat agaaccaggt
     7561 ataaaagtaa aggaaagaga gtatttattt aaagaattcc agcgtaaatt ctcttatttc
     7621 acaatacttc cagaatgtaa aaaggtagca acaaataaca aaaatgaatt atttccttta
     7681 tttgcaccaa aaggattaga gacattaaca ttagaagttt ggtctgaaaa tatagcgctg
     7741 gaaatagaac aagcattact tgatagcgaa attgttttga ctaaaattgg tgaaaattct
     7801 tatgtattga atacagattt cccagtatta ttaaagattg tgagatacaa tattgaaaag
     7861 gtgttacaaa atccatataa aatgcaatat tgtcaaaaat ataaaacaaa tcttgtagat
     7921 gatgttacga aagctgtcta tgctaccgcg ggtgagcgca gtgatgcgac attagcatta
     7981 atcgcaatga aaaattgtga tggacgcgaa aaaattgatc caaaacaaat cattagagag
     8041 ggatttgcac gaacaaatcg tatttcaaca tttattaatt tatttatagg ccaaagtgta
     8101 tctcgtaaaa caattgtaaa tagtgttttt agtctattag aacaaaaagg atttttgaag
     8161 cgtagttgga ataagataaa tttaccttgt acatatgtta atttatcaat tgaacgaata
     8221 tcgaaatttg atttcttacc tattctttca aaaataaaag gaaaagaaat tttatataaa
     8281 ttatatggga atacagagtg gcaaaagatt gattacgtat tattaaatat aaataaacat
     8341 aatgcatttt tatcacaacc atcaaagaga aatgatatgg gggctctttt taaacaatat
     8401 gttttcgaaa cattaatgga aattgtacaa tatgcaaaag aacaaaatga acaagtttac
     8461 ttcattgtag atgcaaattt gagaaaatat tggataaaag agttacaaaa taaagaaatt
     8521 aatacagata ttttacctga tattgttcca gaggtattaa aggttcccaa cttaaatatt
     8581 attagaataa atacaggttt tgatgtacca agctacggtg taatagaatg tgatgatgct
     8641 ctagatagaa taggcttata tgcagatcaa aaagggatgt attatagcac tggtgaatat
     8701 tcacttaatt gtagtgaagt ttttcagcga tatataattg aaattctacc attaggtgta
     8761 aaacctgtag aaagggatta tatcgctaaa atgatacatt acatgtgctg taattctagt
     8821 atgctttcga aaaagaatgt acacatgaca tattccgtac atatggaaaa agtaataaag
     8881 agttatatta ctgacataga tgcaagagaa tttaaagaat ttgatgatga attggatgta
     8941 gatgtagtaa aagtagaaaa gaaagattca attattctac tttaaatact agtaaacaaa
     9001 tcctgctcgt tatatcgatc aggatttgtc atttttttat gcaaataagg atatcatttg
     9061 tcaagtatct tttcgtttac atatttactt tatgtgcaga caggattaat atgtttttta
     9121 gaaatacaaa aactatacca aatggaaaag cgatttttat taattgaaaa aatttgaaag
     9181 gaatggggaa tggtgaagaa attagatgtg ctcttaatgc tactgagcgt cctctttcca
     9241 attgcaggac ttacaaagca aatccctcta gaacaatctg tatatattgg agggctttta
     9301 ttttttacta gtttcggtag ttattttgct aaaaagatgt attcacgtat atgcagctgg
     9361 atagcgtatg cgccatttat aacattactg ttagttattt ggcatcagga tattacaaca
     9421 agctcaatca tagtaaatgc gaaaattgct gcatgtgtcg cgttaattcc atgtttattt
     9481 cgttttcgta catatggact tactttggga ttattctcat tatgggccgc tttactatgg
     9541 gatataaaag aagtacagtc gttagtcata cttgaacgca tgacgagttt aatgacaagc
     9601 cattatgtat atatattact attaattgga ggacttgtat taggaggatt acttgcgatg
     9661 ttcattcacc gcaaagagaa agataataat aaagaaaata taaatctact tggaccaaaa
     9721 aagaaacgca aaagattatc atttaagatt cgtcttccga gattaccgaa attaaagatg
     9781 aaactattta agtttggtgg gaaagaatct aaaagtaaaa ttccagaaaa gatacatgag
     9841 cataagtacg aagaaccagt tgcaacatat aaaatgacag agaaaataga tccatacaaa
     9901 gaaaatgaag ttcaaggtca aacaaggatg gaacgccgaa gaaataggtt taatgcataa
     9961 tgaagtttaa taaccccgta atgacttatc ttaatattga taaatcatta cggttttttt
    10021 atgtaattaa ataaaggttg ctaatgaggg ggggatagca tattttatag gtgagaagtt
    10081 aaataaaata aggggaggat aaaatgctta tcattacata tatatctgct tgtatattat
    10141 tcttagttag ctttttacat gtatattggg gtttcggagg taattggggg acaaatagtg
    10201 ttataccaac taaatcaggg gaaaaggcgt ttacacctcg tgccggaatg acattgttca
    10261 ttgcattatt attaagtatg gcagcgataa ttttgttgca acaagcaaat at
//
