LOCUS       NZ_PVRL01000011        10261 bp    DNA     linear   CON 21-MAY-2024
DEFINITION  Bacillus thuringiensis strain NMTD81
            NODE_11_length_169304_cov_10.5154, whole genome shotgun sequence.
ACCESSION   NZ_PVRL01000011
VERSION     NZ_PVRL01000011.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 169304)
  AUTHORS   Borriss,R., Lasch,P., Wu,L., W,H. and Gao,X.
  TITLE     Genotypic and phenotypic analysis of antagonistic Bacillus spp.
            isolated from rhizosphere soil of plants in Tibet
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 169304)
  AUTHORS   Borriss,R., Lasch,P., Wu,L., W,H. and Gao,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2018) UG, NordReet, Marienstr. 27a, Greifswald
            17489, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: A5 v. A5-2017
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 14.5x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002998815.1-RS_2024_05_21
            Annotation Date                   :: 05/21/2024 01:45:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,177
            CDSs (total)                      :: 6,068
            Genes (coding)                    :: 5,840
            CDSs (with protein)               :: 5,840
            Genes (RNA)                       :: 109
            rRNAs                             :: 11, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 1 (5S, 23S)
            partial rRNAs                     :: 6, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 228
            CDSs (without protein)            :: 228
            Pseudo Genes (ambiguous residues) :: 0 of 228
            Pseudo Genes (frameshifted)       :: 100 of 228
            Pseudo Genes (incomplete)         :: 142 of 228
            Pseudo Genes (internal stop)      :: 67 of 228
            Pseudo Genes (multiple problems)  :: 63 of 228
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:59:29
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 29575
            Orig. end                         :: 39836
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            PVRL01000011.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10261
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10261
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10261
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5261](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5261
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            977..2338
                     /locus_tag="C6351_RS21660"
                     /old_locus_tag="C6351_21660"
     CDS             977..2338
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017763222.1"
                     /locus_tag="C6351_RS21660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6351_21660"
                     /product="glycosyl hydrolase family 8"
                     /protein_id="WP_106082081.1"
                     /transl_table=11
                     /translation="MNGKRNIFTCISIIGIGLASFSNSSFAANVTDNSVQNSIPVVNQQ
                     VAAAKEMKPFPQQVNYAGVIKPTHVTQESLNASVRSYYDNWKKKYLKNDLSSLPGGYYV
                     KGEITGDADGFKPLGTSEGQGYGMIITVLMAGYDSNAQKIYDGLFKTARTFKSSQNPNL
                     MGWVVADSKKAQGHFDSATDGDLDIAYSLLLAHKQWGSNGTVNYLKEAQDMITKGIKAS
                     NVTNNSRLNLGDWDSKNSLDTRPSDWMMSHLRAFYEFTGDKTWLTVINNLYDVYTQFSN
                     KYSPNTGLISDFVVKNPPQPAPKDFLDESKYTNAYYYNASRVPLRIVMDYAMYGEKRSK
                     VISDKVSSWIQNKTNGNPSKIVDGYQLNGSNIGSYPTGVFVSPFIAASITDSNNQKWVN
                     SGWDWMKNKKESYFSDSYNLLTMLFITGNWWKPVPDDKKIQNQINDAIYEGYDN"
     gene            2501..3424
                     /locus_tag="C6351_RS21665"
                     /old_locus_tag="C6351_21665"
     CDS             2501..3424
                     /EC_number="3.2.2.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016087665.1"
                     /locus_tag="C6351_RS21665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6351_21665"
                     /product="nucleoside hydrolase"
                     /protein_id="WP_014895267.1"
                     /transl_table=11
                     /translation="MEKVLFFGDPGIDDSFAIMYGLLHPEIEIVGIVTGYGNVEHIHAA
                     HNAAYILQLANRQDIPVISGATKPLTEEITTYYPEIHGAEGLGPIHVPESVLSIPIYNF
                     GSIAAILVKYGKDLTIVDVGRSTSLAIAFNLWNDLMLNVKAIYFMGGVFLEVGNVTPLA
                     EANVYGDPIASKIVFHQAKNLLIFPLNVTNKAVLTPNVFSYIQANTKNPFHMIMKPMYD
                     YYLSAYQKLNPSIEGPLLHDVVAISGLVNPSFFEFVHRTVNVDTYGGTKGQTFADFRPS
                     SIPEGAHIALEIDEQKFIQDFIKIML"
     gene            3502..3960
                     /locus_tag="C6351_RS21670"
                     /old_locus_tag="C6351_21670"
     CDS             3502..3960
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102696.1"
                     /locus_tag="C6351_RS21670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6351_21670"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_086390554.1"
                     /transl_table=11
                     /translation="MNVQLKVVTRENWEEALKLQVKENQLKFVPSVAVSLAKVYIKPDG
                     DNVEYIPFSIYVGDLMVGFVMHAVVRETSDMYWINGFIIDQKQQGNGYGKAALQESINI
                     IKNTFKSCKEIRLTVHKDNISAKKLYERYSFKSLEQEYDGEQVYRLFV"
     gene            3991..4338
                     /gene="cutA"
                     /locus_tag="C6351_RS21675"
                     /old_locus_tag="C6351_21675"
     CDS             3991..4338
                     /GO_process="GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="cutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160776.1"
                     /locus_tag="C6351_RS21675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6351_21675"
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="WP_001160789.1"
                     /transl_table=11
                     /translation="MQFTEVKIEVFIPEEYIETLRDELNKIGACKTGEYDHCLSYSSVK
                     GYWRPLDEASPFNGEIGRICEGQECKVEIKCKRELVKDALEVINDIHPYETPMIYIIPI
                     LNDYFEQLYIE"
     gene            4355..4801
                     /locus_tag="C6351_RS21680"
                     /old_locus_tag="C6351_21680"
     CDS             4355..4801
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836819.1"
                     /locus_tag="C6351_RS21680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6351_21680"
                     /product="NUDIX domain-containing protein"
                     /protein_id="WP_000836826.1"
                     /transl_table=11
                     /translation="MKSKFHHIVRAVMIKDEKLLVAEYIGHHYFLPGGHVEIGESAENA
                     LIRELREELGVNCSIQQFLGVIENQWQDKEVLHHEINHIFEVESQDLHTDLPPKSSESH
                     LAFQWIDCNKEALNHYEIMPMPLVKELLERKLSDELLNCWISNF"
     gene            5001..5261
                     /locus_tag="C6351_RS21685"
                     /old_locus_tag="C6351_21685"
     CDS             5001..5261
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071342.1"
                     /locus_tag="C6351_RS21685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6351_21685"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_001071342.1"
                     /sec_met_domain="TIGR03601 (E-value: 2.3e-41, bitscore:
                     133.7, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQFQHELQTLNLNDYQTGNVVYWDQQQSQYPYYYIQDDARRCGG
                     CGGCGGRCGGCGGGRCGGCGGRCVGCAGCFGCFNCWNWWII"
     gene            5363..6379
                     /locus_tag="C6351_RS21690"
                     /old_locus_tag="C6351_21690"
     CDS             5363..6379
                     /EC_number="3.1.1.103"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1053: beta-lactamase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288887.1"
                     /locus_tag="C6351_RS21690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6351_21690"
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="WP_000288927.1"
                     /transl_table=11
                     /translation="MYTYEKLISWVENIKEKNQSSAAALCIIKDNKIVLEHYSGYHSNT
                     STSKKVTAPSQFNVASARKSYLGLIIAYALYEGKINSIDDEAIKYFKDFDPVLLGKTTI
                     RHLVTHSHGLEETNDGTIFREFEPGQSWAYRDINVRMMTRLIYQLYNKSFPELLKERVF
                     KPAKFQETGWRIQQDENLVEIINNPNEDPISGIGTVNDGTEKNLFVSAREFAQWGNLHL
                     NQGMIHDKQIVPKEVIKIATSLQSPTFINKELPQNGVFWFIQNEPAQLSELGERIPKGS
                     YQILGITGPTILVIPEYNVVVAKMYNKRYNYGGDNYLYYLREFSNLVADTFSNRIRA"
     gene            6411..6554
                     /locus_tag="C6351_RS32555"
                     /old_locus_tag="C6351_21695"
     CDS             6411..6554
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017657232.1"
                     /locus_tag="C6351_RS32555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6351_21695"
                     /product="hypothetical protein"
                     /protein_id="WP_000653680.1"
                     /transl_table=11
                     /translation="MKDKVNKNKKQNIKDVNTEQNAIYSDPKDAANMQTVPQPKDFDEI
                     EY"
     gene            6835..8043
                     /locus_tag="C6351_RS21700"
                     /old_locus_tag="C6351_21700"
     CDS             6835..8043
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1137: Major
                     facilitator superfamily MFS 1"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814376.1"
                     /locus_tag="C6351_RS21700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6351_21700"
                     /product="OFA family MFS transporter"
                     /protein_id="WP_000814378.1"
                     /transl_table=11
                     /translation="MKQSSINPLLIVLGTIIVQIGLGTIYTWSLFNQPLVSKFGWNLNS
                     VAITFSITSFSLSFSTLFAAKLQKKLGLRKLIATAGIVLGLGLILSSQVSSLPLLYLLA
                     GVVVGYADGTAYITSLSNLIKWFPNRKGLISGISVSAYGMGSLIFRYINGNLIDNLGVS
                     QAFLYWGIIVLLLVLIGSFFLREAIVSNAVTETLHNDYTPREMMRTKQVYLLFFMLFTS
                     CMGGLYLISMVKDIGVQLVGLSAATAANAVAMIAIFNTVGRIILGTLSDKIGRMKIVSA
                     TFIIIGLSVFTLSFIPLNYGIYFACVASVAFCFGGNITIFPAIVGDFFGLKNHSTNYGI
                     VYQGFGFGALAGSFIGAILGGFQPTFIIIGVLSVISFIISILIRPPTAEKKKELKHLHR
                     KVA"
     gene            8174..8599
                     /locus_tag="C6351_RS21705"
                     /old_locus_tag="C6351_21705"
     CDS             8174..8599
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706722.1"
                     /locus_tag="C6351_RS21705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6351_21705"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_000706899.1"
                     /transl_table=11
                     /translation="MKIVQQWVQEDSDYIREKVIEYNQKYLADEEKTPSEKVSFIVRNE
                     KEEIVGGVTAIIFWHHVHVDFLWVSEEYRHEGYGNKLIKLIEEFAIEKGCRLINLDTFS
                     FQAPDFYKKHGYKVIGVSEDHPKGHNHYYLEKRLKSI"
     misc_feature    complement(8782..8800)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 22.4"
                     /tool="antismash"
     gene            8881..9324
                     /locus_tag="C6351_RS21710"
                     /old_locus_tag="C6351_21710"
     CDS             8881..9324
                     /EC_number="3.6.-.-"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757615.1"
                     /locus_tag="C6351_RS21710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6351_21710"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_106082082.1"
                     /transl_table=11
                     /translation="MKKVNVTYAILYDKTNEKILMVKNKGENGSYYTLPGGAVKLGETL
                     EEGVIREVKEETGLHINVKGICSISEAFFEERDHHAIFFNFLGEIIGGETYISRPKEIE
                     EITWMELHIAAPYLRIPEHLLDLLQRKETVPYFFNGTIVHQSS"
     misc_feature    complement(9507..9520)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 24.32"
                     /tool="antismash"
ORIGIN
        1 ggcaattcgt atggggcttg aatcttatac acctgagcat cttactttac tacgcttatt
       61 aatcgcttca tttatacttc tgttgttttc ttttatctac aagttacggc taccggattt
      121 aaaggatatc ccagcaattt ttatgttcgg tgctttagga ttcacttttt atcacattgc
      181 attaaactac ggcgaaaaaa cagtaaatgc tggttctgca agtttaattg tttcggtcac
      241 acctatagta acggcaattc ttgcttctat atttttaaac gaaaaaatga aattaaatgg
      301 ttggatcggt ggtgtaatta gttttatagg aattgctctc atatcattta gtcaaggaga
      361 tgctattcaa ttgaatagtg ggggattatt tatattatta gcagcgattt cagaaagcct
      421 atattttgtt tttcaaactt cttacttaaa aaagtacggc ttcttaccat ttaccatata
      481 tacaatttta tctagtactg tatgtatgct tattttctta ccgggaatgt atacagaaat
      541 actagcagct cctcttgaag tgaatgtgag tgttttatat ttaggcttat ttccaacagt
      601 actcccatat atcgcattgg cctatatcat atctcatgct ggtgctgctg aggcaacaag
      661 ttctctatat ttaacgccag taactgcatg ttttattgct tggttatggt taggagaagt
      721 gccaacttta gtttcgataa ttggcggagg gattactata cttggtatcg tgattgctca
      781 tataccagta ttaagaaaag aaaaacatag gaatttagca aataatcaat ccgtataacg
      841 taattcaata ataattttat gatacttttt catatagttt ctttcttatg ttgataaata
      901 taatattttg tatttcagtt aaatattgta tgttacattt tatgtagtaa gcattttaaa
      961 aggagatgac aacataatga atggaaaaag aaatattttc acatgtattt ctattatagg
     1021 aatcggtcta gctagttttt ctaattctag tttcgcagca aatgtaacgg acaattcagt
     1081 acaaaattct attcccgtag ttaatcaaca agtagctgct gcaaaggaaa tgaaaccatt
     1141 tccccagcaa gttaattatg caggtgttat aaaacctact catgttacac aggaaagttt
     1201 aaatgcttct gtaagaagtt actacgataa ttggaaaaag aaatatttga aaaatgattt
     1261 atcttcttta cctggtggtt attacgtaaa aggagagatt acgggtgatg cggatgggtt
     1321 taagccactt ggaacttcag aaggtcaagg gtatgggatg ataattacag tattaatggc
     1381 tggttacgat tcgaatgccc aaaaaatcta tgatggttta tttaaaacag caagaacttt
     1441 taaaagctct caaaatccta atttaatggg atgggttgtc gcagatagta aaaaagcaca
     1501 aggtcatttt gattctgcta ctgatgggga tttagatatt gcgtattctc ttcttcttgc
     1561 tcataagcag tggggatcaa atggaacagt taattattta aaagaagcac aagacatgat
     1621 tacaaaaggt attaaagcta gtaatgttac aaataatagc cgactaaatt taggagattg
     1681 ggattctaaa aattcacttg atacgaggcc atctgattgg atgatgtcac accttagagc
     1741 attttatgaa tttacaggtg ataaaacttg gcttactgtt attaataatt tgtacgatgt
     1801 ttatacgcaa tttagtaata agtactctcc aaatacaggg cttatttcag atttcgttgt
     1861 aaaaaaccca ccacaacccg cacctaaaga cttcttagat gagtcaaaat atacaaatgc
     1921 atattattat aatgctagtc gagtaccttt aagaattgta atggactatg cgatgtacgg
     1981 tgagaagcga agtaaagtca tttctgataa agtctcttcg tggattcaaa ataaaacgaa
     2041 tggaaatcct tctaaaattg tggatggtta tcaattaaac gggtctaata ttggtagtta
     2101 tccaactggt gtattcgttt cgccatttat tgctgcaagt ataacggata gcaataatca
     2161 aaagtgggta aatagcggtt gggattggat gaagaataag aaagaaagct actttagtga
     2221 tagttataat ttattaacca tgttatttat tacaggaaat tggtggaaac ctgtacctga
     2281 tgataaaaaa atacaaaatc aaataaatga tgcaatttat gaaggatacg ataattaatt
     2341 aaatttgaga atggttactt aagaaaggaa ttagcatacg ctagttcctt tttaatattt
     2401 tgtagaacat tatccattct aaaattcgca tagatatact tgcttttttc atatacatag
     2461 ttattgatga ctctatgttt ttatgagggg agagtatttc ttggaaaaag tattattttt
     2521 cggagaccca ggtattgatg actcatttgc cattatgtat ggtttactgc atcccgaaat
     2581 tgaaattgta ggtattgtaa ctggatacgg aaatgttgag catatacatg ctgcacataa
     2641 tgcagcgtat attttacagt tagcaaatag acaagacatt cctgtcatta gtggtgcaac
     2701 aaaaccactt acagaagaaa ttactacgta ctaccctgaa attcacggag cagaaggatt
     2761 agggcccatc catgttccag aaagcgtatt atctattcca atatacaatt ttggtagtat
     2821 cgctgcgatt cttgtaaagt acggaaaaga tttaacgatt gttgatgtag gaagatctac
     2881 ctcgttagca attgctttta atttatggaa tgatttaatg ctaaatgtaa aagcaatata
     2941 tttcatgggt ggtgtttttt tagaagtagg aaacgttacg ccgttagcag aggcaaatgt
     3001 gtatggagat ccgatagcaa gtaaaattgt tttccatcaa gcaaagaatt tattaatatt
     3061 ccctttaaat gtaacaaaca aagctgtgtt aacaccaaat gtatttagct atattcaagc
     3121 taatacaaag aatccatttc atatgataat gaaaccaatg tatgactact atttatctgc
     3181 ataccaaaaa cttaatccat ctatcgaagg acctttgcta catgatgtgg ttgcaataag
     3241 tggattagta aatccaagtt tttttgaatt tgtgcatcgt acagtaaatg tagatacata
     3301 cggaggtacg aaaggacaaa catttgctga tttccgccct agctctatac ctgaaggggc
     3361 ccatatagca ttagaaatag atgaacaaaa attcattcaa gattttataa aaatcatgtt
     3421 ataagttgtt gtcgaaaaag ggaattattt caatgcgttg aatataaaaa tacatccctc
     3481 tagataaagg agcttacaat attgaatgtc cagttaaagg ttgttacgag agaaaattgg
     3541 gaagaagcat taaagctaca agttaaagaa aatcagttga aatttgttcc atccgtagca
     3601 gtttcgcttg ctaaagtata tataaaacca gatggtgaca atgtagagta cataccattc
     3661 tctatatacg ttggtgacct tatggttggt tttgtaatgc atgcagttgt aagagagaca
     3721 tcagatatgt attggattaa cggatttatt attgatcaaa agcagcaagg aaatggatat
     3781 ggaaaagcag cattacaaga aagtattaat ataataaaaa atacttttaa atcgtgtaaa
     3841 gaaattagat taaccgtaca taaagataat atctctgcaa agaaactata tgaacgatac
     3901 agtttcaaat cattagaaca ggagtatgat ggcgagcaag tatatcgttt atttgtttaa
     3961 ctattaggaa gtaaaggcgt gaaatgaaat atgcaattta ctgaagtgaa aattgaagtg
     4021 ttcattcctg aagaatatat tgaaacatta agggatgaat taaataaaat tggagcatgt
     4081 aaaacgggcg aatatgacca ttgtctttcc tatagttcag tgaaaggata ttggagacct
     4141 ttagacgagg catctccttt taatggagaa attggtcgaa tatgcgaagg gcaagaatgt
     4201 aaagttgaaa taaagtgtaa acgagaactt gtaaaagatg ctcttgaagt tattaatgat
     4261 attcatccgt atgaaacacc tatgatttat attataccaa tactaaatga ctattttgag
     4321 caactataca ttgaataact aggaggaata tcaattgaaa agtaaatttc atcatattgt
     4381 acgagcagtc atgataaaag atgaaaaatt attagttgct gaatatattg gccatcatta
     4441 ttttttacct ggtggccatg ttgaaattgg agaatcagca gagaatgcat taataagaga
     4501 gttaagagaa gaacttggag taaactgtag tatacaacaa tttttaggag tcatagaaaa
     4561 tcaatggcaa gataaagaag tgcttcatca tgaaattaac cacatttttg aagtagagtc
     4621 acaagattta catacggact taccaccgaa atctagcgag tctcatttag catttcaatg
     4681 gatagattgt aataaagaag ccttaaatca ttatgaaatc atgccaatgc ctttagtgaa
     4741 ggaattacta gaaagaaagt taagtgatga actactaaat tgttggatta gtaattttta
     4801 aatcacttta tttatagtaa atacgtccat cgaatgaata taaagaaaat aggctgaaaa
     4861 acaaggtaca tcacctagtg taagttcata ttttgttgat gtacgcattt agtctatagt
     4921 cttgtttcta ctttgttcac aagaatttat taactttaat atttgggcgg aatttatatt
     4981 tataaatgga ggggttctta atgaatcagt ttcaacatga actacaaaca ttaaatctta
     5041 atgattatca aactggtaat gttgtgtatt gggatcaaca acaaagtcaa tatccatact
     5101 actacattca agacgatgca cgtcgttgcg gaggatgcgg aggttgtggt ggacgctgtg
     5161 gcggttgtgg cggcggtaga tgtggtggct gtggtggacg ttgcgtaggt tgcgctggtt
     5221 gttttggttg ctttaattgc tggaactggt ggattattta aatatagtac atcattaata
     5281 ttttatatga aagaaaggtg taaatactgt ttatactttt ctttcatgct ataattttgt
     5341 attagtgtta agggggattt atttgtatac atacgaaaag ttgatttctt gggtagaaaa
     5401 tataaaagaa aaaaatcaaa gttctgctgc agcactttgt attataaaag ataataaaat
     5461 cgtgctagag cattatagtg gttatcactc aaatacatct acaagcaaga aagtaacagc
     5521 accttcacaa tttaacgttg cttctgctag aaaaagttat ttaggattaa taatagcgta
     5581 tgcgctttat gaggggaaaa taaactctat tgatgatgaa gcgataaaat attttaaaga
     5641 ctttgatcct gtattgcttg gtaaaacgac gataagacat ttagtaacac attcgcacgg
     5701 attagaagaa acgaatgacg ggacaatttt tcgtgaattt gaaccgggac aatcatgggc
     5761 gtatagagat attaatgtaa gaatgatgac acgtcttatt tatcagctat ataacaaaag
     5821 ttttcctgaa ttgttaaaag agcgtgtttt taaacctgct aaatttcaag aaacaggatg
     5881 gagaatacag caagatgaaa atttggttga aattattaat aatccaaatg aagacccgat
     5941 aagtggaatt ggtacagtaa atgatggtac tgaaaaaaat ctatttgtct cagctagaga
     6001 atttgcgcag tggggcaatc ttcatctaaa tcaaggcatg atacatgata aacaaatcgt
     6061 tccaaaagaa gttataaaaa ttgctacgag tttgcagagt ccaacattta taaacaaaga
     6121 gctaccacaa aatggggtgt tttggttcat ccaaaatgag cctgcacaat taagcgaact
     6181 tggtgaacgt attccaaaag ggtcgtatca aatattaggg attactggac cgactatttt
     6241 agtaatacct gaatataatg tagttgttgc aaaaatgtat aataaaagat ataactacgg
     6301 cggtgataat tacttatatt atttacgtga atttagtaat ttagtcgctg atacatttag
     6361 taaccgtatt agggcataat tactcctata acaaaaaggg gtgattgaaa gtgaaagata
     6421 aagtgaacaa aaataaaaag caaaatataa aagatgtaaa tacagagcaa aatgcaattt
     6481 atagcgatcc taaagatgca gctaatatgc aaactgtacc gcagccgaaa gattttgatg
     6541 aaatcgaata ttaatttttc atccgactat atgatgaatg aaaaatacgt cttaagtctc
     6601 ataacattta agaaattatt tgataacata tatacataag catttgaaaa tgtcttatct
     6661 taacaaagct ttctttagaa atatctgata taacctgcaa aaggcacttc tttatgaagt
     6721 gtctttttgt gtatatgaac ttaattacca ttttccgtac agtatcttgt aatttatgat
     6781 ggatatacta actgtgaaaa ttcatgtcaa gataaaagaa ggggacaagt tagtatgaaa
     6841 caaagttcta taaatccgtt actaattgtt ctaggtacaa tcattgttca aattggcctt
     6901 ggaacaattt atacatggag tttatttaat caaccccttg taagtaagtt tggatggaat
     6961 cttaattcag tagcgataac tttctccata acaagttttt ctttatcatt ctcaacttta
     7021 tttgcagcaa agttgcagaa aaaattagga cttcgaaaac ttattgctac tgcaggaatt
     7081 gttttaggac tcggcttaat acttagttca caagtttctt ctttaccact attatattta
     7141 ttagctggtg tcgttgttgg ttatgcggat ggaacagctt atattacatc actatctaat
     7201 ttaattaaat ggtttccaaa tcggaaaggg cttatttcag gtatatctgt atcagcgtat
     7261 ggaatgggca gcttaatctt tagatatata aacggaaatc ttatcgataa ccttggtgta
     7321 tcacaagcat tcttatattg gggtattatc gtgttacttt tagtgttaat cggatcgttc
     7381 ttcttacgtg aggcaattgt aagtaatgct gtaactgaaa cattacacaa tgactatact
     7441 ccgcgtgaaa tgatgcgaac gaaacaagta tatctgctat tttttatgtt attcacctcg
     7501 tgtatgggtg gtttgtattt aataagtatg gtaaaagata tcggcgtaca actcgttgga
     7561 cttagcgcag caactgctgc aaacgccgtt gctatgattg caatctttaa tacagtaggt
     7621 agaattattc ttgggacgct atcagataaa atcggccgaa tgaaaattgt ctctgcaacg
     7681 tttattatta taggattgtc agtctttact ttaagtttta ttccgctaaa ttacggaatc
     7741 tattttgctt gtgtagcaag tgtcgccttt tgcttcggtg gtaatataac tatattccca
     7801 gctattgtcg gagatttctt tggattaaaa aaccatagta caaattacgg cattgtctac
     7861 caaggtttcg gatttggtgc gcttgcagga tcatttattg gagcgatact cgggggattt
     7921 caaccaactt tcattataat cggtgtttta agtgttattt ccttcattat ttcaatatta
     7981 atccgtccac caactgcaga aaagaaaaag gaactaaaac atttacatcg gaaagttgct
     8041 taattttaaa aagagtcctc atgttagggc tcttttttat tccattaact ttgcttcttt
     8101 actaactcat taaagacagg acaatatcat tataaaattg aatagataag taaaaaggag
     8161 cgtgacactt aaaatgaaaa tagtacagca gtgggtacaa gaagatagtg attacataag
     8221 agagaaagta attgaatata atcaaaaata tctcgcagat gaagaaaaaa caccttcaga
     8281 aaaggttagt ttcatagtaa gaaatgaaaa agaagaaatc gtaggtgggg taacagcaat
     8341 aattttttgg caccatgtac acgttgactt tctatgggtt tctgaagaat ataggcatga
     8401 aggttacgga aacaagttaa taaagcttat tgaagaattt gcaattgaaa aagggtgtag
     8461 gttaattaat ttagatacct ttagtttcca agctcctgat ttttataaga aacatggata
     8521 taaagtaatt ggagtaagtg aagatcatcc gaaaggacat aatcattatt atttagaaaa
     8581 acggctaaaa agtatataga atttgttaac attagtattt ggaatacaaa tgtaatcaag
     8641 gcgaggttat tattaatttt agttttaatt taatcaattt tcatgctaaa acttcaagaa
     8701 cgttctttgg ataatcttcc taagatacgt tctttcttta tatatcagtg gcccattctt
     8761 attaactcca ccaatagcgg taaaggcaag acttaactaa cttatatata ttaaaaggag
     8821 ttaatggtag aatggataga agacagaaaa ttccttttat taaaaataga ggaggtacat
     8881 atgaaaaaag ttaatgttac atacgcgatt ttgtacgata aaactaatga aaagatctta
     8941 atggtaaaaa acaaagggga aaatggttct tattatacat taccgggtgg cgcagttaaa
     9001 ttaggagaaa ccttagaaga aggagtaatt cgggaagtaa aagaagaaac agggttacat
     9061 ataaacgtaa aagggatttg ttctatcagc gaagcctttt tcgaggaaag agatcatcat
     9121 gcaattttct ttaattttct aggcgaaata atcggcgggg agacatatat atcaagacca
     9181 aaagaaatcg aagagattac ttggatggaa ttacatatag cagcaccgta tttacgcata
     9241 ccagaacatt tactagattt attacaaagg aaagaaacag taccttattt ttttaacgga
     9301 acaatcgttc atcaatcttc ataaaaatat tatctcatgt aaggcgggga aaacatggag
     9361 ttcatcgtta atcacaaaca atttactcaa gcactttcag aagttaacaa agcgatatca
     9421 acaaaatctt taattcctat attatctggt ataaaaataa ctgcagatca atctggaatt
     9481 actttaattg caagtaactc gaatattttt attgaaaaat tcattcctgt ttcaattgaa
     9541 ggcgaaaaaa ttgcaactat tttaaaggca ggaactattg ttgtacctgc aaagtatttc
     9601 attgaaataa taaagaaaat gccaagtgat atagcaataa aaagtaagaa tgagcaaatt
     9661 attaagatac aatcagaaga aatcacatta agtttaaatg gatttcctgc agatgagttt
     9721 ccaaatgtac cgcttataga caatcatgca gaaatcaagg tagagacaga gcaattgatt
     9781 gaagtattta aacaaacagt ctttgcggta gcgaaaaatg aatctagacc tgttcttact
     9841 ggggtgcata tagagttatc taataataag tttatttgtg ctgcaactga ctcacataga
     9901 ctagcgatac gtgaaacgcg actttcctca gatgtaaaag caaattgtat tgtaccaagc
     9961 gcaaccatta gtgaacttct aaaattaatg aacagcaatt cggaattcgt atatatttac
    10021 ctttcagaga gtcatattat atttacgctt ggaacaacta cattatattc aagactaatt
    10081 gaagggaaat atcctaatat ttctaacctt attccaaatg actttaaaac gattattaac
    10141 atagatagaa aaaagatatt acaaggtgta gaccgatcaa gtttattagc aagtgaatgg
    10201 gccaataaca atgttaactt agaaatcata aacgaatcta caattaaaat ttcttcaaac
    10261 g
//
