LOCUS       NZ_PVQM01000001        10345 bp    DNA     linear   CON 06-NOV-2024
DEFINITION  Bacillus atrophaeus strain GBSC56 NODE_1_length_1636870_cov_39.3765,
            whole genome shotgun sequence.
ACCESSION   NZ_PVQM01000001
VERSION     NZ_PVQM01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus atrophaeus
  ORGANISM  Bacillus atrophaeus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales; Bacillaceae;
            Bacillus.
REFERENCE   1  (bases 1 to 1636870)
  AUTHORS   Borriss,R., Lasch,P., Wu,L., Wu,H. and Gao,X.
  TITLE     Genotypic and phenotypic analysis of antagonistic Bacillus spp.
            isolated from rhizosphere soil of plants in Tibet
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1636870)
  AUTHORS   Borriss,R., Lasch,P., Wu,L., Wu,H. and Gao,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2018) UG, NordReet, Marienstr. 27a, Greifswald
            17489, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: A5 v. A5-2017
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 39.4x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002998055.1-RS_2024_11_06
            Annotation Date                   :: 11/06/2024 02:04:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,249
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 3,986
            CDSs (with protein)               :: 3,986
            Genes (RNA)                       :: 92
            rRNAs                             :: 7, 8, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 1 (5S, 23S)
            partial rRNAs                     :: 8, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 171
            CDSs (without protein)            :: 171
            Pseudo Genes (ambiguous residues) :: 0 of 171
            Pseudo Genes (frameshifted)       :: 105 of 171
            Pseudo Genes (incomplete)         :: 120 of 171
            Pseudo Genes (internal stop)      :: 23 of 171
            Pseudo Genes (multiple problems)  :: 68 of 171
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:35:15
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 469971
            Orig. end                         :: 480316
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            PVQM01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10345
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10345
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10345
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5345](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5345
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    106..119
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.11"
                     /tool="antismash"
     gene            complement(181..381)
                     /locus_tag="C6W22_RS02090"
                     /old_locus_tag="C6W22_02090"
     CDS             complement(181..381)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017697615.1"
                     /locus_tag="C6W22_RS02090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6W22_02090"
                     /product="hypothetical protein"
                     /protein_id="WP_106046531.1"
                     /transl_table=11
                     /translation="MNGIEFLESVSQLGQIILLFSGYALYWRLGKAKKERKLTKYETFL
                     RFFVMFGIFAWALSFLALHLW"
     misc_feature    complement(544..557)
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 21.07"
                     /tool="antismash"
     gene            complement(1017..2054)
                     /locus_tag="C6W22_RS02100"
                     /old_locus_tag="C6W22_02100"
     CDS             complement(1017..2054)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF027588.5"
                     /locus_tag="C6W22_RS02100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6W22_02100"
                     /product="DUF4917 family protein"
                     /protein_id="WP_106046532.1"
                     /transl_table=11
                     /translation="MTELYDFKALSEKHPEILDNLLTGNGFSIEFDSNFRYGGLYEYLQ
                     TKELFNVREQNLFEAFETTNFELVLNSLLKAHTINETLGKDTDYLDESYQTIKNLLIMA
                     VKEIHPAYWLLDNDKLAWSFNIFKKNIFTTNYDLLSYWALLAIRNKKLIRDGFWPRDDQ
                     KLCFQSDTFQNNHFKLFYLHGALHLYENRGDVIKVKSSGANLMDDITKEYERGNFPVYV
                     SEGASEKKLAQIKNNSYLSHCYHELLNSDGGITILGQGLNPEYDKHLIDAIKNSKVKFI
                     AYGVYDTPDTTKDYIIETTKKHFGDLEDKELHFYDSKNFFNSVKEIAYQDKPFWGMMPP
                     HFPKI"
     misc_feature    2462..2476
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 19.27"
                     /tool="antismash"
     gene            3246..3434
                     /locus_tag="C6W22_RS02110"
                     /old_locus_tag="C6W22_02110"
     CDS             3246..3434
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010330622.1"
                     /locus_tag="C6W22_RS02110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6W22_02110"
                     /product="hypothetical protein"
                     /protein_id="WP_088117215.1"
                     /transl_table=11
                     /translation="MKIVVDFWFDNENVRTAKVIAESEKALIKELQECEHWYEFEDGKR
                     IANVNMRLVTQFDVKRK"
     gene            3571..4139
                     /locus_tag="C6W22_RS02115"
                     /old_locus_tag="C6W22_02115"
                     /pseudo=""
     CDS             3571..4139
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611387.1"
                     /locus_tag="C6W22_RS02115"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="C6W22_02115"
                     /product="IS3 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MEVKQKAVDMIM"
     gene            4323..4976
                     /locus_tag="C6W22_RS02120"
                     /old_locus_tag="C6W22_02120"
     CDS             4323..4976
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016764.5"
                     /locus_tag="C6W22_RS02120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6W22_02120"
                     /product="YIP1 family protein"
                     /protein_id="WP_106046533.1"
                     /transl_table=11
                     /translation="MDKKHNFITLIHHFIFSPKDFLLIKYSSDKNYRHSWFVFLAVLSL
                     FLGFYLAIFPTLSTLINNSFRVNGIDYTTDMNTLIMIITLVLGIIQFTSMVLVSFFIIH
                     IIGILIGGAATAKEFFPSFIYSIIPIGIKFTILIIFSFFLGSDFAFSVIYPNSQQPLGI
                     ILNIIIDPFFWWSTILGCIALKIIHQISYKKTIIIISITMSLGIISLFSTSLFL"
     gene            5001..5345
                     /locus_tag="C6W22_RS02125"
                     /old_locus_tag="C6W22_02125"
     CDS             5001..5345
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03651"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03651.1"
                     /locus_tag="C6W22_RS02125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6W22_02125"
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="WP_106046534.1"
                     /sec_met_domain="TIGR03651 (E-value: 9.9e-10, bitscore:
                     35.2, seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNSNLFKIVSVTSVVSLFAIATFLMGSSEGIAGLASIDFSSGNQ
                     FALDLSTNLGISRKTAYVAIGVIMTTGDILTILSLLAVVLGGTGLITAAMVATAKKLAK
                     KYGKKYAAEW"
     gene            5417..7111
                     /locus_tag="C6W22_RS02130"
                     /old_locus_tag="C6W22_02130"
     CDS             5417..7111
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="C6W22_RS02130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="C6W22_02130"
                     /product="hypothetical protein"
                     /protein_id="WP_106046535.1"
                     /transl_table=11
                     /translation="MLNKRVLIANFKLTYRRTLNRLIYFFPSSENLLKSNKILFVFAPF
                     FLVYLFILYKASQTLLTGVPEHILTLGTNFIILFFVLNILKSSTDIIHRYVYPEDFLLL
                     HTLKTDPKKILLSRFFTTIFLNILFKLTVVLYPILFFIIFSTHDRIYLLLSFPSLLIFI
                     VGLTSLYSSLTYIVYSKFLHNILNSRSKSLLSLLGIACLSAISTTFLGWIGLNYFYQND
                     LLEAIGNFIYLFKLDTILTTNWLPTNWFLLSTSDVHLFFICSIGYIALGCLFVFITFNL
                     ILKVLIIKEAVPNNLLPKLKPIKLPFLSFLQKALPRQNIAILKKDLTALLRANFTIKKR
                     LVLSINMIAIELGVLIGLSLNQMWFDFSDIFVIYPILFAVTYNTSLLGEGLLGVTSADA
                     ERENLYVYKSSGASIFKLIAGKSLLHLSVILILMNTLYLFILLLFNLDIMTQFILFIMI
                     NSISLIIGAGQIIGTFLYPRLDWENFEDIGSSVKASFFEHTILGVLLAFYLQLYGITGV
                     LVTFDIINRGIFFSIIVTGTILFVIGIGFFYYFWIKRIGTKNWEMKT"
     gene            7108..7815
                     /locus_tag="C6W22_RS02135"
                     /old_locus_tag="C6W22_02135"
     CDS             7108..7815
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012235.5"
                     /locus_tag="C6W22_RS02135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6W22_02135"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_088117219.1"
                     /transl_table=11
                     /translation="MNIENVSFKYKDKLILESINLKIEKGKVYGLLGPNGAGKSTLLKC
                     ILGIVKPYEGKITIKGETLESERSSYLKYIGYVPDAPFIYHYLTPREYLEFVADLQDVP
                     YEIKNDRIDLLLSQFNLKDNENDMISTFSFGMKHKLSVAGAILHEPEILILDEPLGAFD
                     PPTTKMMKSFLKRYAEQGNTVIMSTHLVNIAQQLCDKVGILINGTLVKEYNHLTDIKDF
                     EDLIIRDMDTILK"
     gene            7812..8366
                     /locus_tag="C6W22_RS02140"
                     /old_locus_tag="C6W22_02140"
     CDS             7812..8366
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014052.5"
                     /locus_tag="C6W22_RS02140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6W22_02140"
                     /product="stage II sporulation protein M"
                     /protein_id="WP_106046536.1"
                     /transl_table=11
                     /translation="MKELIKRNKRILYIASSFYIVGLVLGVIFNNIIPSLSQDNVQNIT
                     VLGYFTHNFAANLFLIFSMFTFGILTSILLLLNGFLVGLSSLHSITDHGNDVLFILTAL
                     VPHGIFEIPAMILSGALGFKLLDFVYMKIRGIYFLKKQFLLDFLYFVIILFLLTLLAAV
                     TEALITPYLLNKLTLGGSYVY"
     misc_feature    8387..8405
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: medium, score: 24.31"
                     /tool="antismash"
     misc_feature    complement(8388..8406)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: medium, score: 25.03"
                     /tool="antismash"
     misc_feature    complement(8390..8403)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.01"
                     /tool="antismash"
     gene            8826..9218
                     /locus_tag="C6W22_RS21970"
     CDS             8826..9218
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015557.5"
                     /locus_tag="C6W22_RS21970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SLC13 family permease"
                     /protein_id="WP_395939679.1"
                     /transl_table=11
                     /translation="MLTLIGLLIILTIVVLLILEKTSPIVAMVMVPLIGALIAGFGIND
                     IQTFFEEGIIKVLPVATMFIFALLFFGILQDTGFFEPVIKALIKLTKGNVVTVSMGTAA
                     IGRISHLDGAGATTFLLTVPALLPHI"
     gene            9334..9555
                     /locus_tag="C6W22_RS21975"
     CDS             9334..9555
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="C6W22_RS21975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_311187278.1"
                     /transl_table=11
                     /translation="MVPLIPIQLIGVVFVLGMAFILGKREQKRIQVKIAAKKVAAVQLE
                     GADWDRAFNSTSETGTSFYLPLWCPFLY"
     gene            9582..9902
                     /locus_tag="C6W22_RS21980"
     CDS             9582..9902
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="C6W22_RS21980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="SLC13 family permease"
                     /protein_id="WP_233529404.1"
                     /transl_table=11
                     /translation="MIGLSLALLINYPNVKMQMERIKVHAPSALVMAAVIFSAGSFLGI
                     LEGTGMLKAIAVDSVYILPAFLLPFLHIIVGFLGVPLDMLTSTDAYYYALLPIVKSAFL
                     FE"
ORIGIN
        1 tggcaatagc aagaaagaaa accgacgcca gaaaaaacaa tcttttcaac atgtgtgaac
       61 tccccctttt tttgaaatgg taaagcaatt taatagtaac atacattttt tcaataaaaa
      121 gggattttga tttagaatta acaaaataga cataattagt aaaatatcga cagaagattg
      181 ttaccacaag tgcaaagcaa gaaacgaaag tgcccatgca aatatgccga acattacaaa
      241 gaatctaaga aaagtttcat attttgtaag ttttcgttct ttttttgctt tacctaatct
      301 ccaataaagg gcgtagccgg agaacaataa aattatttgt cccagttgag atacggactc
      361 taaaaactct ataccattca ttgatttcac ctcttattta attatcgcag acggaaaaac
      421 cgtttccaac agttatataa aaattgagca atccgtaata tgtctaatgg tataagaagc
      481 aacgtttccc ttattcatat gaagcgcagt tcctgccggt gtgtaataaa aatgctctaa
      541 gaattacatt cgatccatgt ttgcttcttc tgatctcatt tataacgaat ccaattgata
      601 aataaagccc aactcatatg agttttgctt tattaaataa tccatatttt cttttcttta
      661 tcccatgaaa gagcgccttt gcggattagg ttttttgtgg cttcacgtat ttcctgttca
      721 cttttcccgg tctttctttt agttctggca aagtcgggtt ctttccatat aaacgcatgt
      781 tcacaatgat ctgatataac ttacgttcaa aattagtcac acaatatcaa ctcctagatc
      841 taggtacatc gaaaaaacaa ttgtaagtat tagttggttt tgtcatatta gtggccctcc
      901 atcttcagct ttttcctttt gaagtgatcc ataacagatc ggataagaaa gcaaaacctg
      961 aatcgttatt tgaaataaat aaaaaatccc cttaaatttt caaggggatt taggtattaa
     1021 atctttggga aatgaggagg catcataccc cagaaaggtt tatcttgata tgctatctct
     1081 tttactgagt tgaaaaagtt ttttgaatca taaaaatgaa gttctttatc ctctaaatca
     1141 ccaaaatgtt tttttgtggt ttcaataata taatctttag ttgtatctgg cgtatcataa
     1201 accccatatg ctataaattt cacttttgaa tttttaatag catctataag atgtttatcg
     1261 tattcaggat ttaaaccttg acctaaaata gtaataccac catcactatt taaaagctca
     1321 tgatagcaat ggcttaagta actattattt ttgatttggg ctagtttttt ctctgatgca
     1381 ccttctgaga cataaacagg aaagtttcct ctctcgtact cttttgttat atcatccatc
     1441 aaatttgctc ctgaagattt tactttgatg acgtcaccac gattttcata aagatgtaat
     1501 gcaccatgta agtaaaacaa tttaaaatga ttattttgaa aggtatctga ttgaaaacaa
     1561 agcttttgat catctcgcgg ccaaaaacca tccctaatta gttttttatt tctaatagct
     1621 agtaaagccc agtacgacaa caaatcgtag ttagtagtga agatgttttt cttgaaaata
     1681 ttaaagctcc aagctaattt atcattatct aaaagccaat atgcgggatg tatttctttt
     1741 acagccataa taaggaggtt tttaatcgtt tgataacttt cgtctaaata atctgtgtct
     1801 ttgcctaatg tttcgttaat tgtatgtgcc ttgagaagag aatttagaac aagctcaaag
     1861 tttgtagtct caaaagcttc aaataagttt tgttctctaa cattaaaaag ttcttttgtt
     1921 tgtaaatatt cgtatagacc gccgtatcta aaattcgaat caaattctat tgagaagcca
     1981 tttcccgtga gtaaattatc taatatttca ggatgctttt ctgataatgc cttaaaatca
     2041 taaagttctg tcatatgaat atcctctttc cttattcaaa tatattttaa tcttacttgt
     2101 tgctatgtcg gctgcacgac gagcaatacg tttcccgagt gtgctgttct tagcagcgtt
     2161 aacatcacct aaaattttag ctggctttta gtgcgctgat cttactttgc ttagaatcat
     2221 atcggataca atctccttat tttaagtgag ttactatatt ataccatcag ttcttttttt
     2281 agcttgaaag tttttgacca aaatcatgac caaaatttga ccaaaaaata ttgagaatta
     2341 tagagactct atttcagatt aaaaataata aagcactgac aaagcctttg ttttcagggt
     2401 tttcagtgct atacattatt tatttaaatt gaaaattatg ttccgcacgc aagtacatcc
     2461 ttgggagcgc gaacagtata tgtctcagta ttaatctttc aatcccttgg cactattggt
     2521 gtcaggggat ttttgatttt ggtcaataga atgggcttga acaaaccatt tcaggaacac
     2581 acccggaaca tattacatta ttttacaatg acaatgaaag cgtacgaaat tttcggagga
     2641 caaaacttcc aaatatgaaa tcacttaaat ttgtaatttt gggtgctctc tttagaaatg
     2701 gttattagaa aacgaaaaag aggaagcgtt tgatttctat ataaagaata taaatgcaaa
     2761 gcgaatcacg ctttaaaaaa cttgtgttta cagatagacg cattaataga tttgaatgag
     2821 gaaatgatga agggcgttat actatagctg aatatttaaa tattataggt ctcgactaag
     2881 aaatagttag ttctttaaat aacactttta attcttcacc ttatctattc aacatgcggg
     2941 atgaattaag aagtgaattt tgctgatcca ataaaaaatt aagaatatca aattgtttcg
     3001 cttaaaattc gttaatatga tattagttca aaatcataag cgacttccac tattggtggt
     3061 cgtttttata ttctctgtaa actgctttcg gtaaatctca ggatagacaa ttggcggtta
     3121 atgctgcttg agtgcgatgc cagtttagaa agaatatgtg atttaattgg aatcgttcga
     3181 caaattttgc aaactgtttc atttctagac ctcttgtctg ataataaagc aagaggtgaa
     3241 agtttatgaa aattgtagtt gatttttggt ttgataatga aaatgtaaga acagctaaag
     3301 taattgcaga atctgagaaa gcacttataa aagaattgca agaatgtgaa cattggtatg
     3361 agtttgaaga tggtaagcgt atagcaaatg ttaatatgcg acttgtaacg caatttgatg
     3421 tgaaaagaaa ataaaattca taaaaaagca tccgggactg gccccataag atgagacaaa
     3481 ttaaaaacac cttcaagttt gaaaacggat ggttgttatc cgaaattaga cttggaggtg
     3541 tttttctatg gggacaagag aagttatccg atggaagtca aacagaaggc tgtagacatg
     3601 atcatgtagg aactgaatat caagaataag acgcaggttc agacatgggt cagctggtat
     3661 aaggctgggg atacacaccg acagccagtt ggaaagcaat acacttacgg aaaaggtccg
     3721 gagtattctt ccgaattaga gaaacagcag gcagagaatc gttatatgag aaaacaaaat
     3781 aaagttttaa aaaagtacaa acgaattgga aaggaagttg atagggaaac gtcaatcaaa
     3841 ctggtagaag gattgaggga aacaatgacg gtacaggcca tctgtgtcca tttaggtatc
     3901 tcacgcgctt catattatag atggaaaaag gcaaagggac gcccgctgat aatgcctcca
     3961 tcgaatcgtt tcattcctca ctaaagtctg aaacgttcta tcttaacagc attgaccgaa
     4021 ctacgaccgc catcgtagaa cgcactgtca aagaatacat ttattattat aacaacattc
     4081 gtattcaaac gaaactaaat aaccaatcac cggtaaacta tcggcaattg gctgtataaa
     4141 ggtgttttga tccctgtctc aaaaacaggg gtcagtccca gaggcactct tttcgatttt
     4201 tacgaaacaa ttcaacctta aatttatttt ataaataaaa ttccatcttg aacaaacatt
     4261 gtaaaatatg atacattatt taatagaatt aacaaaaaat actttttata aaaggagcat
     4321 ttatggataa aaaacacaac tttattactc tcatacatca tttcatattt tctcctaagg
     4381 actttctttt aatcaagtat tctagtgata agaattatcg tcattcttgg tttgtcttcc
     4441 tagctgtatt gagtctgttt ttaggctttt atttagctat cttcccaaca ctctctacct
     4501 tgattaataa ttcatttcga gtcaatggaa ttgattatac aactgatatg aatacactca
     4561 ttatgattat tacattagta ttaggtatta ttcaatttac ctcgatggtt cttgtaagct
     4621 tttttattat acatattatt gggatattaa ttggcggggc cgctacagcc aaagaatttt
     4681 tccctagttt tatttattca atcataccca taggcatcaa atttacaata ctcattattt
     4741 tttctttctt tttaggtagt gatttcgcct tttcagttat ttacccaaat tctcaacagc
     4801 cactaggtat tatcttgaat attattatcg accctttctt ttggtggtct accattttag
     4861 ggtgtattgc cttaaaaata atacatcaaa tctcttataa aaaaaccatt ataatcatta
     4921 gtattacaat gtctttaggt attatttctc ttttttcaac cagtttattt ttataaactg
     4981 aaaggaggtg aaatttcaag atgaaaaata gcaatctttt taaaattgta agtgttacaa
     5041 gtgtagtttc tctctttgcc attgctactt tccttatggg ctcttcagaa ggtattgcag
     5101 gtttagcttc tattgacttt agttcaggta atcagttcgc tttagacctt tcaacaaacc
     5161 taggaattag tagaaaaaca gcctatgtag caatcggtgt tattatgaca acaggtgata
     5221 tattaacaat tctcagccta ttggctgttg ttttgggcgg aactggtcta attacagctg
     5281 caatggtagc tacagcgaaa aagctagcta aaaaatacgg caagaaatat gctgctgaat
     5341 ggtaaaatga tcaaatggaa aataaggagg agtataattt actcctcctt attttcgttc
     5401 taggaggaat atacacatgc tcaacaaaag ggttctcatt gcaaacttta aactaaccta
     5461 tagacgaact ttaaatcgct tgatttattt ttttccatca agcgaaaatt tacttaaatc
     5521 aaataaaatt ctatttgtct ttgcgccctt tttcttagtg tatctattta ttctctataa
     5581 agcctcacag actcttttaa ctggagttcc agagcatatt ctaactctag gtactaactt
     5641 tatcattctc ttttttgttt tgaatatatt aaaatcatcg actgatatca tacatcgata
     5701 tgtatatcct gaagattttc ttcttctcca tactttaaaa acggatccaa aaaaaatttt
     5761 attatcaagg ttcttcacaa caatttttct aaacatcctg tttaaattaa cagtagtgtt
     5821 ataccctata ctctttttta taattttttc tacacatgat cgtatatatc ttttgttatc
     5881 atttccatca ttgctaatat ttattgttgg gctaacaagt ctttattcat cgcttacata
     5941 catcgtttat tctaaatttc tacataacat tttaaattca cgatcaaaaa gcttgttatc
     6001 ccttttagga attgcctgtt tatctgccat ttcaactact tttcttggtt ggattggact
     6061 taattatttt tatcaaaatg atctccttga agctattggc aatttcatat atctatttaa
     6121 attagatacg atactgacca ccaattggct tcctacaaat tggtttttac tttcaacaag
     6181 tgatgtacat ttatttttta tatgttctat aggttatatt gctttaggtt gccttttcgt
     6241 ctttattacc tttaatttaa ttttgaaagt attgattata aaagaagcag tacctaataa
     6301 tcttttacca aaattaaaac caatcaagtt gcctttttta agtttcttac aaaaggcctt
     6361 accacggcaa aatattgcta ttctaaagaa agaccttact gcattattaa gagctaattt
     6421 tacaataaaa aaacgactgg tactttccat taatatgatt gcaatagaac taggtgtatt
     6481 gataggtctt agtttaaatc aaatgtggtt tgatttctca gatatctttg ttatctaccc
     6541 aattttattt gctgtgactt ataacacatc tttattagga gagggattac ttggcgtaac
     6601 ttcagcagat gcagagcgtg agaaccttta tgtttataag agttcgggtg cttctatttt
     6661 taaattgatc gcaggaaaat ctttattgca cttatctgtg attcttattt taatgaatac
     6721 gttatatcta tttatcttat tgttattcaa cttagatatt atgacgcaat ttatattgtt
     6781 catcatgatt aactccatca gtcttatcat aggtgctggg caaattattg gaacatttct
     6841 ctatcctcgt ttagattggg agaactttga agacattggt tcatctgtta aagcatcatt
     6901 ttttgaacat actattcttg gagtgttact ggcattttac cttcagttgt atggtatcac
     6961 aggcgttctc gttacatttg atattattaa taggggaata ttcttttcaa tcattgtgac
     7021 aggtaccatt ttatttgtta taggtattgg attcttttat tacttttgga ttaaaagaat
     7081 aggaacgaaa aattgggaga tgaaaacatg aacattgaaa atgtgtcttt caaatataaa
     7141 gataaactga tccttgaatc tattaattta aagatagaga aaggcaaagt gtacggctta
     7201 cttggaccaa atggagctgg taaatcaact cttttaaaat gtatccttgg tatagtgaaa
     7261 ccttacgaag gtaaaatcac tatcaaagga gaaaccttag aatctgaaag atcctcttac
     7321 cttaaatata taggatacgt tccagacgca ccttttatct atcattattt aacacctaga
     7381 gagtaccttg agtttgttgc tgatttacaa gatgtccctt atgagattaa aaatgataga
     7441 atagatcttt tgttaagtca atttaacttg aaagataatg aaaatgacat gatatctaca
     7501 ttttcttttg gtatgaaaca taagttatct gttgcaggag ccatacttca tgaaccagaa
     7561 atattaatat tagatgaacc attaggtgca tttgatcctc ccactacaaa gatgatgaaa
     7621 agttttctca aacgttatgc tgaacagggg aatactgtca tcatgtctac acaccttgta
     7681 aacatagcac aacaactatg tgataaggtt ggaatactga ttaatggtac tttagttaaa
     7741 gaatataatc accttacaga cataaaggat tttgaagatt tgattataag ggatatggat
     7801 acgatactaa aatgaaggaa ttaataaaaa gaaataagcg tatactctat atagcaagct
     7861 ccttttacat agtgggttta gttcttggtg tgatttttaa caatattata cctagcctta
     7921 gtcaagataa tgttcaaaat atcacagtat taggttattt tacacataat tttgcagcga
     7981 atttatttct cattttttca atgtttacat ttggaatttt aacatctatc ttattactat
     8041 tgaatggctt cttagtcggt ctatcttctt tacatagtat caccgaccat ggaaacgatg
     8101 tcttatttat attaactgct ttggttcctc acggtatttt tgagattcct gcaatgattt
     8161 tgtcgggagc acttggtttc aaattgttag atttcgtata tatgaagata aggggtattt
     8221 attttttaaa aaaacagttt ttactagatt ttttgtactt tgttattata ctatttttat
     8281 tgacattact tgctgctgtt acagaagcat taataacgcc ttatttactt aacaaattaa
     8341 cattaggagg ctcttatgtt tattaataaa aagcagatag ataaattact ggatgatcat
     8401 acagaagaac aaaaagaaac gaaaagtaaa gttaatactt catttataat agtattttat
     8461 ttttcaatac tactatcaat ctgtactttc ttttttaatt ataggtactt tttgattact
     8521 ttgtttggcc cgaccatttt agtaactttt atatccccac ctttacaatt caaaaaaggt
     8581 atttatagaa ttttttcggc cctatattta atacttttaa ttacagttac tttcttttgg
     8641 ttaaagtaag tgattaattc tcaataaaaa aacgtcctta attgatgttt tttcatttta
     8701 tatcgtttta aataaataat ttgaattatt ttttgacatg ttccatcccg cactatgaat
     8761 gggttctcca tcctttttta tcattgtata ggtatgtaaa cggtttattg tagggggagg
     8821 ggcttatgtt aacgcttatt ggtttgctga ttattttaac gattgtcgta ttgctgattt
     8881 tggaaaaaac aagtccgatt gttgccatgg tcatggtgcc tttgataggg gcgctcattg
     8941 ccggatttgg tatcaatgat attcaaacct tttttgaaga agggattatc aaagtactgc
     9001 cagtggcgac catgtttatc ttcgctcttt tattttttgg tattttacaa gatacagggt
     9061 tctttgaacc tgtaatcaaa gcattaatca agctgacaaa ggggaatgtt gtcaccgttt
     9121 ccatgggtac agctgcaatt gggaggatct cacacttaga cggtgcagga gctacaacct
     9181 ttttattaac tgtacccgca ctgcttccgc acatatgagt ccttacttac tgttgttgtt
     9241 aattgggact agtgccgact taaccaatat ggttccgtgg ggtggaccaa ctggacgggc
     9301 agtaagtgta ctaaatgtga gtccgtcaga attatggtac cgctcatacc gattcagctc
     9361 attggtgtag tgtttgtgtt agggatggcc tttattttag gaaaaagaga acaaaagcga
     9421 atacaagtga aaatagctgc aaaaaaggtc gcagccgttc aacttgaagg tgcggattgg
     9481 gatcgcgcct tcaactccac cagtgaaaca ggaacttcat tttatttgcc gctgtggtgt
     9541 ccattcttgt attaaatatc atccctgcga gttatacatt tatgattggg ctatcactcg
     9601 cacttttaat taactaccct aatgtgaaaa tgcaaatgga gcgtatcaag gtacatgcac
     9661 ccagtgccct tgtaatggca gctgttattt tttctgcagg atcattttta gggatccttg
     9721 aagggactgg tatgctgaag gcaattgcgg tggatagtgt ttatattctg ccagcatttc
     9781 ttctgccatt tctacatatc attgttggtt ttcttggagt gcctcttgat atgttaacaa
     9841 gcacagatgc ttattattat gcacttttgc ctattgtgaa atcagctttc ctgtttgaat
     9901 aatagcaaaa cgatcacaca tcaattccat ttctgacatg aggtgacttg acacaatgac
     9961 agcaagccct tcttccttcg caagccttct caagtaatct cgtatcattc gaataccctc
    10021 tggatcaagt ccattggtcg gctcatctaa aatgagcagt ttcgggttta tgtatgagtg
    10081 cctgcgcaat gcccaaacgc tgcttcattc ctaatgaaaa tgtacggaca cgcttatgtt
    10141 tcacatgctg caaactcaca agctgcaaca gctcatcaat tcgtccttca tctactttcc
    10201 catttattct tgcaaaatgg gcaaggtttt gtcttgccgt taaatgatta tacatttctt
    10261 ggttctcaat aatcgcacct acatcttgga tagcatgctg ataagacgtc tgaatactat
    10321 gtccattaat cagaatgtca ccaga
//
