LOCUS       NZ_CP023477            10231 bp    DNA     circular CON 18-JUL-2024
DEFINITION  Streptococcus mutans strain LAR01 chromosome, complete genome.
ACCESSION   NZ_CP023477
VERSION     NZ_CP023477.1
KEYWORDS    RefSeq.
SOURCE      Streptococcus mutans
  ORGANISM  Streptococcus mutans
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2088369)
  AUTHORS   Abranches,J., Lemos,J., Kajfasz,J. and Aviles-Reyes,A.
  TITLE     Whole genome sequence and phenotypic characterization of a Cbm+
            serotype e strain of Streptococcus mutans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2088369)
  AUTHORS   Abranches,J., Lemos,J., Kajfasz,J. and Aviles-Reyes,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-SEP-2017) Oral Biology, University of Florida, 1395
            Center Drive POBOX 100424, Gainesville, FL 32610, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: celera v. 9/01/15
            Genome Coverage                   :: missing
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002995555.1-RS_2024_07_18
            Annotation Date                   :: 07/18/2024 01:10:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,086
            CDSs (total)                      :: 2,002
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 41 of 72
            Pseudo Genes (incomplete)         :: 35 of 72
            Pseudo Genes (internal stop)      :: 29 of 72
            Pseudo Genes (multiple problems)  :: 25 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:47:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1631885
            Orig. end                         :: 1642116
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP023477.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Dr. Jacqueline Abranches.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10231
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="7"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10231
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10231
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5231](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5231)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            30..551
                     /locus_tag="CO204_RS08275"
                     /old_locus_tag="CO204_08230"
     CDS             30..551
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283970.1"
                     /locus_tag="CO204_RS08275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CO204_08230"
                     /product="YgjV family protein"
                     /protein_id="WP_002264875.1"
                     /transl_table=11
                     /translation="MVTLMILPIAMLFSALGAFCLAYSSFTKEKQHMLLWQISDYIFTI
                     IANLLLGGYTGALTISVSIVRNALILKKKNTKVITICLVLIQIALGTYVNNLGLVGYLP
                     IISSVSYTLTTFLTSKMQFLRWLIIENMSLWFIYDLTINAYPAVCMDILIILSTLLALY
                     KHRKKAGHSL"
     gene            complement(658..1479)
                     /locus_tag="CO204_RS08280"
                     /old_locus_tag="CO204_08235"
     CDS             complement(658..1479)
                     /EC_number="3.1.3.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1179: hydrolase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351286.1"
                     /locus_tag="CO204_RS08280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CO204_08235"
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="WP_032505481.1"
                     /transl_table=11
                     /translation="MVKLIAIDMDGTLLNSQKEIPQENIAVIQEAARSGYKIVLCTGRM
                     QTGVEPYFEQLGLAAEQEYAILNNGCSLHTINRDWQLLTYHDLNFNGVSYLYDLLEGYP
                     EIDLTLTADRDYLVLADQVSELVAYDASLVFTQAQTVSLNDVKHAAKPVFQAMYLGESD
                     RLDAFQQKFECKLAEKFTTVRSQPYIFEVMPQSITKATGLKELAQKLRVSRTDIMAIGD
                     ALNDLEMLKAAGFSVAMGNASPEVKAAADLVTGSNDDAGVAQAIAKYVLNK"
     gene            complement(1726..1929)
                     /gene="copZ"
                     /locus_tag="CO204_RS08285"
                     /old_locus_tag="CO204_08240"
     CDS             complement(1726..1929)
                     /codon_start=1
                     /gene="copZ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263706.1"
                     /locus_tag="CO204_RS08285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CO204_08240"
                     /product="copper chaperone CopZ"
                     /protein_id="WP_002264873.1"
                     /transl_table=11
                     /translation="MEKTYHIDGLKCQGCADNVTKRFSELKKVNDVKVDLDKKEVTITG
                     NPSKWSLKRALKGTNYELGAEI"
     gene            complement(1942..4170)
                     /locus_tag="CO204_RS08290"
                     /old_locus_tag="CO204_08245"
     CDS             complement(1942..4170)
                     /EC_number="7.2.2.-"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1037: heavy metal
                     translocating P-type ATPase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002264872.1"
                     /locus_tag="CO204_RS08290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CO204_08245"
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="WP_002277960.1"
                     /transl_table=11
                     /translation="MSEEVFLIDGMTCASCAINVENAVKKLDGIESAVVNLTTEKMTID
                     YDAAKVSEADVTKAVAGAGYGAKVYDPTTAESQEDREEHKLAGIKKRLLWTSIFTIPLF
                     YIAMGSMVGLPLPNFLAPSSAPLTYAMVLLLLTIPVIVLSWSFYDNGFRSLFKGHPNMD
                     SLVSLATTAAFLYSLYGTYHVYLGHTHHAHHLYYESVAVILTLITLGKYFETLSKGRTS
                     DAIKKLMHLSAKEATLIRDGEEIKVPIEQVQIRDQILVKPGEKIPVDGRVLSGHSAIDE
                     SMLTGESIPIEKMADSPVYAGSINGQGSLTFEAEKVGNETLLSQIIKLVENAQQTKAPI
                     AKIADKVSAVFVPVIITIAILTGLFWYFVMGQDFTFSMTISVAVLVIACPCALGLATPT
                     AIMVGTGRAAENGILYKRGDVLELAHQINTIVFDKTGTITQGKPEVVHQFSYHDRTDLV
                     QVTASLEALSEHPLSQAIVDYAKKEGTRLLAVEDFTSLTGLGLKGCVADETLLVGNEKL
                     MRQENISLEQAQADFKAATAQGQTPIFVASDGQLLGLITIADKVKNDSAATVKALQNMG
                     VEVAMLTGDNEETAQAIAKEVGITFVISQVLPQEKTQAILDLQAEGKKVAMVGDGINDA
                     PALATADIGISMGSGTDIAMESADIVLMKPAMLDIIKALKISRATIINIKENLFWAFIY
                     NVLSVPIAMGVLYLFGGPLLDPMIAGLAMSFSSVSVVLNALRLKVVKL"
     gene            complement(4167..4610)
                     /locus_tag="CO204_RS08295"
                     /old_locus_tag="CO204_08250"
     CDS             complement(4167..4610)
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /GO_process="GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002264871.1"
                     /locus_tag="CO204_RS08295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CO204_08250"
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="WP_025988626.1"
                     /transl_table=11
                     /translation="MTTISNAEWEVMRVVWAKQMTSSSEIIAILSRTYCWSASTIKTLI
                     TRLSEKGYLISQRQGRKYIYSSLISEEEALEQQVSEVFSRICVTKHQALIRHLIEETPM
                     TLSDIEKLEALLLSKKANAVPEVKCNCIVGQCSCHEHLEVTSK"
     misc_feature    4656..4671
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 24.41"
                     /tool="antismash"
     misc_feature    4682..4698
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 23.54"
                     /tool="antismash"
     misc_feature    4687..4700
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.34"
                     /tool="antismash"
     misc_feature    complement(4802..4816)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: medium, score: 23.87"
                     /tool="antismash"
     gene            complement(5001..5231)
                     /locus_tag="CO204_RS08300"
                     /old_locus_tag="CO204_08255"
     CDS             complement(5001..5231)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019320866.1"
                     /locus_tag="CO204_RS08300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CO204_08255"
                     /product="bacteriocin class II family protein"
                     /protein_id="WP_002275977.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 7.8e-16,
                     bitscore: 55.1, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTQAFEQFNVMDNEALSTVEGGGMIRCALGTAGSAGLGFVGGMG
                     AGTVTLPVVGTVSGAALGGWSGAAVGAATFC"
     gene            complement(5710..6060)
                     /gene="rbfA"
                     /locus_tag="CO204_RS08305"
                     /old_locus_tag="CO204_08260"
     CDS             complement(5710..6060)
                     /GO_process="GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /gene="rbfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262599.1"
                     /locus_tag="CO204_RS08305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CO204_08260"
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="WP_002278845.1"
                     /transl_table=11
                     /translation="MSNHRIERVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGDLS
                     LAKVYYTIMSDLASDNQKVQIGLEKAKGTIKRELGKNLTMYKIPDLTFIKDESIEYGNK
                     IDQMLRDLEKRN"
     misc_feature    complement(6269..6286)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: medium, score: 19.83"
                     /tool="antismash"
     gene            complement(6294..9044)
                     /gene="infB"
                     /locus_tag="CO204_RS08310"
                     /old_locus_tag="CO204_08265"
     CDS             complement(6294..9044)
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0003743 - translation initiation factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0003924 - GTPase activity [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /GO_process="GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="infB"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1190: GTP-binding protein LepA"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285798.1"
                     /locus_tag="CO204_RS08310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CO204_08265"
                     /product="translation initiation factor IF-2"
                     /protein_id="WP_019313085.1"
                     /transl_table=11
                     /translation="MSKKRLYEIAKEIGKESKEIVEKAKSLGLEVKSHASSVEESDAKR
                     IVESFTVSVEPKAVTPTSKVEKEAKAQEGSVAAESKAAVTKPAGRPRPQNRNFKAEREA
                     RAKAEAERRQNNGERRNQNKGQNNRQKDNRNHGSQDRRNDSRNNRNRQNDNRRDNRNHF
                     QNRQEASKSQPTGPRFDFKARAAALKAEQNAEYSRQSEARFHEAQEAKRQAAQAKEKAK
                     KLNQKEQPTVEAAATAAPQAQPQTVEQVTHPAAVDTRRKKQARPDKSRDFSHENEDGPK
                     QNKHKKNRNKQNQVRNQKNSNWNKKNKKSKNNRNHNANLKPVTERKFHELPKEFEYTEG
                     MTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDADTIELLMVDYGIEAHQKVEVDTA
                     DIERFFVEDDYLNPKNMVERAPVVTIMGHVDHGKTTLLDTLRNSRIATGEAGGITQHIG
                     AYQIEEGGKKITFLDTPGHAAFTSMRARGASVTDITILIVAADDGVMPQTIEAINHSKA
                     ADVPIIVAINKIDKPGANPERVIGELAEYGVISTAWGGDSEFVEISAKFGQNIEELLET
                     VLLVAEIQELKADPTVRAIGTVIEARLDKGKGAVATLLVQQGTLHVQDPIVVGNTFGRV
                     RAMTNDLGRRVKVAAPSTPVSITGLNEAPMAGDHFAVYEDEKAARAAGEERAKRALLKQ
                     RQLTHRVSLDNLFDTLKAGEVKSVNVIIKADVQGSVEALAASLLKIDVEGVKVNVVHSA
                     VGAINESDITLAEASNAVIIGFNVRPTPQARQQAEADEVEIRLHSIIYKVIEEVEDAMK
                     GMLDPEFEEKIIGEALIRETFKVSKVGTIGGFMVTNGKITRDSSARVIRDGVVVFDGKL
                     ASLKHYKDDVKEVGNGQEGGLMIENYNDIKIDDTIEAYIMEEIKR"
     gene            complement(9065..9367)
                     /locus_tag="CO204_RS08315"
                     /old_locus_tag="CO204_08270"
     CDS             complement(9065..9367)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262601.1"
                     /locus_tag="CO204_RS08315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CO204_08270"
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="WP_002280994.1"
                     /transl_table=11
                     /translation="MNNSKKLSNLLGLAQRAGCVISGEELVVKAIQTGQAQLIFLAKDA
                     GSNLTKKVTDKSNYYNIEVSTVFSALELSIAIGRNRKVLAIVDTGFSKKMRTFME"
     gene            complement(9360..9656)
                     /locus_tag="CO204_RS08320"
                     /old_locus_tag="CO204_08275"
     CDS             complement(9360..9656)
                     /GO_function="GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531117.1"
                     /locus_tag="CO204_RS08320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CO204_08275"
                     /product="YlxR family protein"
                     /protein_id="WP_002263922.1"
                     /transl_table=11
                     /translation="MAKTRKIPLRKSLVSGEIIDKRDLLRIVKTKEGDVFIDPTGKKNG
                     RGAYIKLANEEALEAKKRKVFNRSFSMEIPESFYDELIAFVDHKVKRRELGLE"
     misc_feature    complement(10031..10045)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.66"
                     /tool="antismash"
ORIGIN
        1 tgaagagtat taatttttga caatcaaaaa tggtaacttt aatgattctt ccaattgcaa
       61 tgcttttttc agccctcggt gctttttgcc tcgcttattc ttcttttacc aaagagaaac
      121 agcacatgct actttggcaa atcagcgact atatttttac gattattgct aatcttttac
      181 tgggtggcta tacaggcgcc ctaaccatta gtgtttccat cgtcagaaat gcacttattc
      241 ttaagaaaaa gaatacaaaa gttattacca tctgtttagt cctcattcaa attgctttag
      301 gtacttatgt gaataatttg ggcttagtcg gttacttgcc cattatttct tctgtctctt
      361 acactttgac aaccttttta acatccaaaa tgcaattttt gcgctggttg attattgaaa
      421 acatgtctct ctggttcatt tatgacctca ctatcaacgc ctaccctgct gtttgcatgg
      481 atattttgat cattctctca acactgctgg ctttatataa acatcgaaaa aaagcaggac
      541 attccttgtg agttggcttt gtcctgcttc tcatttttta tttaattgat cctgaacttt
      601 ctattttcta gtgttaagca gataaaaacg atttcccagt tcctttttca tgatttatca
      661 cttatttaag acatacttgg caatagcttg agcaacacca gcatcatcat tagaaccagt
      721 cacaaggtct gctgcagcct taacttctgg actagcattt cccatagcca cactgaaacc
      781 tgctgctttg agcatttcaa gatcattaag tgcatctcct attgccataa tatcagtcct
      841 tgaaactctt aatttctgag ctaattcttt caaaccagta gcttttgtaa tgctttgagg
      901 catcacttca aaaatgtaag gctgacttct tacagtagta aatttttctg ctaatttgca
      961 ctcaaatttt tgctggaaag catctaagcg atcgctctct cctaaataca tagcctgaaa
     1021 aactggttta gcagcatgct ttacatcatt taagctcaca gtctgtgctt gcgtaaatac
     1081 cagactcgca tcataagcta ctaattctga tacttgatca gccaaaacca gataatccct
     1141 atcagcagtc aatgttaaat cgatttcagg ataaccctca agcaaatcat acaaataaga
     1201 tactccatta aagtttaaat catgataagt cagtaactgc caatctctat taatggtgtg
     1261 aagagagcag ccattattta gaatcgcata ttcttgttca gcagctaagc ctaattgttc
     1321 aaagtagggc tcaacaccag tctgcatacg gcctgtgcaa agaacaattt tatagcccga
     1381 acgagctgct tcttgaataa cagcaatatt ttcttgcggg atttcttttt gactattcaa
     1441 aagcgtgcca tccatatcaa tagcaatcag tttaaccata atcatataac cttttctaaa
     1501 acattccact atctagtgta tcacaaatca aggaaactgg cacagcaagt tattataatt
     1561 ctgtatattc tacaataatt gaaacagata actatgacaa tgctataaat tcattttaca
     1621 gcagaaaaaa aactggtttt ccagtctttt ccctctttgt gaccatctga taagatgcaa
     1681 tgaaataact gtgtctttac agtttttaag gtatatggag cctacttaaa tttctgctcc
     1741 caattcataa ttggttcctt tcagtgctcg tttaagagac cacttgcttg gatttcctgt
     1801 aatcgtgact tcttttttat caaggtcaac tttgacatca ttgactttct ttaattctga
     1861 aaagcgtttg gtgacattgt cagcgcagcc ttggcatttt aagccatcaa tatgatatgt
     1921 tttttccatg ataattctcc tttataattt tacaacttta agacgcaggg catttagaac
     1981 aacagaaaca gagctaaagc tcatggctag accagcaatc attggatcca gcaaaggtcc
     2041 gccaaagaga taaagtactc ccatagcaat aggaactgac agaacattat aaataaatgc
     2101 ccagaaaaga ttttctttaa tattaataat agtagcacga ctaattttca gtgccttaat
     2161 aatatcaagc attgcaggtt tcatcaagac aatatccgcg gactccatgg caatatctgt
     2221 tccagagccc atggaaatgc caatatctgc tgtcgcaagt gccggagcgt cattaatacc
     2281 gtccccaacc atggcaactt tcttgccttc cgcctgcaaa tcaagaatgg cctgtgtttt
     2341 ttcctgcggc aaaacttgac tgataacaaa agtaatcccc acttctttgg caatagcctg
     2401 cgcagtttct tcattatcac ccgtcagcat ggccacttca acccccatat tctgtaaagc
     2461 tttaactgtt gctgcactgt cattctttac cttatccgca atcgtaatca gtcctaacaa
     2521 ttgaccatca ctggcaacaa aaatgggtgt ttgtccctga gctgttgctg ccttaaaatc
     2581 tgcttgagcc tgttctagag agatattttc ttgacgcatc aatttttcat taccaacaag
     2641 caaagtttca tcagcaacac agcctttcag tcctagccct gttagagaag taaagtcttc
     2701 cactgcaagt aaacgagtcc cttctttttt agcataatca acaatggctt gactaagggg
     2761 atgttcagat aatgcttcta aagaagctgt cacttgcact aaatcagttc gatcatgata
     2821 agaaaattga tgaacaactt ctggtttacc ttgagtaata gtgcctgttt tatcgaaaac
     2881 aatcgtatta atctgatgcg ccaattccaa gacatcacca cgtttataaa ggataccatt
     2941 ttcagcagca cgacctgtac caaccataat agccgttggc gttgcaagac ccaaagcaca
     3001 agggcaggca atgacaagaa cagcgacact gattgtcatt gaaaaggtaa agtcttgtcc
     3061 catgacaaaa taccagaaga gaccagtcaa aatagcaatc gttataataa caggtacaaa
     3121 gacagcagat accttatcag caatcttggc aatgggtgcc ttagtttgct gagcgttctc
     3181 aaccaattta ataatttgtg aaagcaaggt ttcattgcca actttttcag cctcaaaagt
     3241 cagacttccc tgaccattga ttgacccggc ataaacaggg ctatccgcca ttttttcaat
     3301 aggaatagat tcccctgtta acatggattc atcaatagca gaatgccctg acaggactcg
     3361 accatccaca ggtatttttt caccgggttt gactaaaatt tgatctctga tttgcacttg
     3421 ctcaatagga accttaatct cctcgccatc acgtatcaga gtagcctctt tagcagacaa
     3481 atgcattaat ttttttatcg catctgaggt tcgacctttc gatagggttt caaagtattt
     3541 ccctaaagta atgagagtta aaataacagc taccgattca taatagagat ggtgagcatg
     3601 atgtgtatgt cccaagtaaa catggtaagt tccataaaga ctgtaaagaa acgccgctgt
     3661 tgttgctaag gacactaatg agtccatatt aggatgacct ttaaaaagcg atctaaaacc
     3721 attgtcatag aagctccaac ttaacacgat aaccggaatt gtcaaaagaa gcaaaaccat
     3781 cgcataagtg agcggagcgc tgcttggtgc taaaaagtta ggtaagggca agccaaccat
     3841 acttcccata gcaatataaa agaggggaat ggtaaaaata gaagtccaca aaagacgttt
     3901 tttaatacct gctaacttat gttcttcacg atcctcctga ctttcagccg tcgttgggtc
     3961 ataaacttta gcgccatagc ctgcaccagc aactgcctta gtaacatctg cttcactaac
     4021 cttagcggca tcgtaatcta tcgtcatttt ttcagttgtc aaattgacta ctgcgctttc
     4081 aataccgtct agttttttaa cggcattttc aacattgatg gcacaagagg cacaggtcat
     4141 gccatctatc aaaaatactt cttcactcat tttgatgtca cctccaaatg ttcatggcaa
     4201 gaacattgcc cgacaataca attacactta acttcaggca cagcatttgc ttttttggac
     4261 aatagcagag cttccaattt ttcaatatca gacaaagtca taggcgtttc ctcaattaag
     4321 tgtctaatca aagcttgatg cttcgtgaca caaatgcgcg agaaaacttc cgacacttgc
     4381 tgctctaatg cctcctcttc tgaaatcaaa ctagaataga tgtattttct tccttgacgc
     4441 tgactgatca agtagccttt ttctgataaa cgcgtgatca acgttttaat cgttgaagcg
     4501 gaccagcaat aagtacgact taagatagca ataatttcac tgctgctagt catctgctta
     4561 gcccagacaa cacgcattac ttcccattcc gcatttgaaa tagttgtcat ttgagctcct
     4621 ttcatctaca tttgtagata tattataacc tctttgtcta catttgtcaa caaaaactga
     4681 tttttaattt tttgacaaaa tatgacccta cttatcagct ttttaaaaga gcaaaaaaag
     4741 aggcgataaa ccttagtttc tgcctcttca ctcaaacaat gaacagttct tcaataataa
     4801 tttttcaaaa tgatacagtc ctcattagct cttgtgattc ttgttaattt taaaccataa
     4861 gactaacaaa cctaacagac ctagtaaatt aatgctataa atgattggcg agagctcctt
     4921 aaaaagattt tttactgcca taaataacaa gaataataaa ggcagacttc cgatcaataa
     4981 ggatcttcta tttttcatgt tcaacaaaaa gtagcagcac ctacagctgc tccagaccag
     5041 cctcctaaag ccgctccaga tactgtacca acgactggga gagtaactgt accagctccc
     5101 atacccccta caaatcctaa acctgcagaa ccagctgtgc caagtgcaca tctaatcata
     5161 ccaccaccct caacagttga aagtgcttca ttatccatta cgttaaattg ttcaaatgct
     5221 tgtgtattca tatgatagat acctcttttc catttttaat tttttgtctg gttagccaac
     5281 cgacatctct aatatagcaa attatttttc aaaatggagc aaaatatcct aaacggtagc
     5341 tattttgtcc taaacggtta aaaatgacgt tataatgcct gtaataatag tcaataaaaa
     5401 ttaaaaatag ttttcgccta ctcgcatcgc aactgactac aaacatactg gagcacgaca
     5461 agctcaaaaa agtcaaaagg cttgggaggc cttctgagat aaaaatagaa gtagataagc
     5521 tgatatcgtc agcacaacaa cctattattc tggtttttaa agagtacaga caggtctact
     5581 tcgaaaataa tcgttttttg gttgatggaa aaagccttcg ttggacttat aatcctaacg
     5641 aaggcttgat acctattttc gtacttttac agcaattaac aagcagacag aagaaacttt
     5701 tttgtttact taatttcttt tttctaaatc ccgcagcatt tggtcaatct tattaccata
     5761 ctcaatagat tcatctttga taaaagttaa atcgggaatt ttatacatgg tcaaattttt
     5821 tccaagttca cgtttaatgg tccctttagc cttttcaaga cctatttgaa ctttctgatt
     5881 atcagaagca aggtcgctca tgatagtata gtaaaccttt gccagagata aatcacctaa
     5941 catttggaca tcagtaatag tgacaccttg aacacgtggg tcacgtacct tcttttgcaa
     6001 aatctcatta acttcgcgct taatttccat accaacacgt tcaatacgat gattagacat
     6061 tctcttttcc tttcttttaa ttttacaatt ttcttttttc accacgacaa aaaatatctt
     6121 gtaaaagctc acttcattcg ctcatcttct gaaaaaagct aggccagatg acctagcttt
     6181 attgtttatc tttgaatttc aaaagaaatc atatgacgtt catctaaggc aacacgacag
     6241 cggctgtact tgcgtatgcc tagaagtgtt aacaaaggag taactaaata ttattatctt
     6301 ttaatttctt ccataatgta agcctcaata gtatcatcaa ttttgatgtc attgtaattt
     6361 tcaatcatga gaccgccttc ttgaccatta ccaacctctt taacatcatc tttataatgt
     6421 ttcaaactag cgagcttacc gtcaaagaca accacaccat cacgaataac acgagcactt
     6481 gaatcacgcg taattttacc atttgttacc atgaagccgc caatagttcc cactttagaa
     6541 actttgaagg tctcacgaat aagcgcttca ccaataattt tttcttcaaa ctctggatca
     6601 agcatacctt tcatggcatc ttctacttct tcaattacct tataaataat ggaatgcaaa
     6661 cgaatttcaa cttcatctgc ttcggcttgc tgacgggcct gtggagttgg gcgaacattg
     6721 aaaccaatga taacagcatt ggaagcttct gccaaggtga tatctgattc attgatggca
     6781 ccaacagctg agtggaccac attaactttc acaccttcaa cgtcaatctt caaaagagag
     6841 gctgcaagag cttcaacgga accttgcaca tctgctttga taataacatt aacagattta
     6901 acctcaccgg ctttgagcgt atcaaagaga ttgtcaagac tgacacgatg agtcaattga
     6961 cgttgtttca gcagagcacg tttagctctt tcttccccag cagcacgcgc agccttttca
     7021 tcctcataaa cggcaaaatg gtcacctgcc attggggctt cattcaaacc agtaatagag
     7081 actggcgttg atggagcagc aaccttaaca cgacgtccaa ggtcattagt catagcacgc
     7141 acacgtccaa atgtgttccc aacaacgatt ggatcttgaa catgcagcgt tccttgttgt
     7201 actaaaagcg tcgctactgc accttttcct ttatcaaggc gagcttcgat aactgtacca
     7261 atagcacgaa cagttgggtc agctttcagt tcttggattt ctgctactag aagaacagtt
     7321 tctaaaagtt cttcaatatt ttgaccgaat ttagctgaaa tttcaacaaa ttcagaatcg
     7381 ccgccccaag cagttgaaat aacgccatac tcagctaact caccgatgac acgttctgga
     7441 ttagcgcccg gcttatcaat tttattaata gcaacaataa ttggaacatc agcagctttg
     7501 gagtggttaa tagcttcaat cgtttgtggc ataacaccat catcagcagc tacaataaga
     7561 atggtaatat cagttaccga agcgccgcgt gcccgcatac ttgtgaaggc tgcatgtccg
     7621 ggtgtatcaa ggaaagtaat tttctttcca ccttcttcaa tttgataggc cccaatatgt
     7681 tgggtaattc ctccagcttc acctgtcgct atacgggaat tacgcaatgt atcaagcaat
     7741 gttgtcttac catggtcgac atgccccata atcgttacaa cgggcgcacg ttcaaccata
     7801 tttttcggat taagataatc atcttcaacg aagaaacgtt caatatcagc tgtatcaact
     7861 tccacttttt gatgtgcttc aatgccataa tcaaccatta ggagttcaat cgtatcagca
     7921 tccaaagatt ggttttgagt tgccataact cccatcataa agagtttttt tacaatttcg
     7981 gcaggctcac gcttaattcg ttttgcaatt tcagcaaccg tcataccttc tgtatactca
     8041 aattcctttg gtagttcatg gaatttgcgt tcagtcacag gtttcagatt ggcattatga
     8101 ttacggttat ttttagattt tttatttttc ttattccaat tactattttt ttgatttctc
     8161 acttggtttt gtttattccg atttttctta tgtttatttt gttttggtcc gtcttcattc
     8221 tcatgtgaaa aatcacgtga tttatctggg cgagcctgtt ttttgcgacg tgtatctaca
     8281 gctgctggat gtgtcacttg ttcaactgtc tgaggctgag cttgtggcgc tgcagttgct
     8341 gcagcttcaa ccgtcggctg ctctttctga tttagcttct tagctttttc tttagcttga
     8401 gcggcttgac gtttagcttc ttgagcttca tgaaaacgtg cttcactctg acgcgaatat
     8461 tccgcatttt gttctgcttt taaggctgca gcacgtgctt tgaaatcaaa acgcggtcct
     8521 gtcggctggg attttgatgc ttcttgtcga ttttgaaaat gattacgatt atcgcgacga
     8581 ttgtcatttt gacgattgcg attattgcgg ctatcattac gacgatcttg ggaaccatga
     8641 tttcgattgt ctttttgccg attgttctgt cctttatttt ggtttcgacg ttcaccatta
     8701 ttttgacgac gttcagcctc tgctttggca cgcgcttctc gttctgcttt aaaattacga
     8761 ttttgcggtc ttggacgccc tgcaggcttt gtaacagctg ctttagattc cgctgcaaca
     8821 gatccttctt gtgctttggc ttctttctca actttagaag ttggagtaac agccttgggt
     8881 tccacagaga cagtaaaact ttctactata cgtttagcat cactttcttc cacacttgaa
     8941 gcatggcttt taacttctag gcccaatgat tttgcctttt caacaatttc tttactttct
     9001 ttgccaattt ccttggcaat ttcgtataat cttttcttcg acaaatcttg tcctcctatt
     9061 ctttttattc cataaaggtc ctcattttct ttgaaaatcc agtatccaca atagcaagaa
     9121 cttttctgtt tctgccaata gcgatactta attccagtgc tgaaaacact gtggagactt
     9181 ctatattata atagttactt ttatccgtta cttttttagt tagattagaa ccagcatctt
     9241 tggctaaaaa gatgagctgt gcctgtccag tttgaatagc tttaacaact aattcttcac
     9301 ctgaaataac acatcctgct ctctgggcca aacctaataa gtttgataac ttttttgaat
     9361 tattcaagac ctaactctct tcttttaacc ttatgatcaa caaaagcaat taattcatca
     9421 taaaagcttt caggaatttc catggaaaaa ctgcgattaa agacttttct ttttttagct
     9481 tctaaagctt cttcgttagc aagtttaata taagcccctc gcccattttt cttaccagta
     9541 ggatcaataa aaacatcacc ttccttggtt ttaacaatcc gaagcaaatc acgtttatca
     9601 ataatctctc ctgaaacaag agactttctt aaaggtattt ttcttgtctt tgccatcaaa
     9661 cacctctgac tttctatttt gcatcttctg ctgcaaattc ttgagagagt tcagaaactt
     9721 cctcagcagg agcctcttca taagtagcct cttgggtttc atcttgtgct gcccactcgg
     9781 cttccatttc ttcaaattca ctagcagact taatatcaat acgatagcca gtcaaatgag
     9841 cggctaaacg gacattttga ccacgacggc caatagcaag tgacagctta ttgtctggta
     9901 caacaactgt tgcatgtttg tcatcttctt catcaaagag aacttgatca acctcagcag
     9961 gagcaacagc attataaatg aattctgctg gatcatcaac ccactcaata acatcaacat
    10021 tctcttccat tggaacccaa agtccagttt tttgatcaaa acgtttagga tggaatttac
    10081 tggtcacttt cttaatgttg gcacctttgc ggccaactat agttccaata gcatcaacat
    10141 taggattatg actgcgaact gcaaccttcg ttcgatcacc agcttcacgt gccacgctca
    10201 taatttcaac agtgccatca aaaacttcag g
//
