LOCUS NZ_CP023477 22499 bp DNA circular CON 18-JUL-2024 DEFINITION Streptococcus mutans strain LAR01 chromosome, complete genome. ACCESSION NZ_CP023477 VERSION NZ_CP023477.1 KEYWORDS RefSeq. SOURCE Streptococcus mutans ORGANISM Streptococcus mutans Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae; Streptococcus. REFERENCE 1 (bases 1 to 2088369) AUTHORS Abranches,J., Lemos,J., Kajfasz,J. and Aviles-Reyes,A. TITLE Whole genome sequence and phenotypic characterization of a Cbm+ serotype e strain of Streptococcus mutans JOURNAL Unpublished REFERENCE 2 (bases 1 to 2088369) AUTHORS Abranches,J., Lemos,J., Kajfasz,J. and Aviles-Reyes,A. TITLE Direct Submission JOURNAL Submitted (19-SEP-2017) Oral Biology, University of Florida, 1395 Center Drive POBOX 100424, Gainesville, FL 32610, USA COMMENT ##Genome-Assembly-Data-START## Assembly Method :: celera v. 9/01/15 Genome Coverage :: missing Sequencing Technology :: PacBio ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI RefSeq Annotation Name :: GCF_002995555.1-RS_2024_07_18 Annotation Date :: 07/18/2024 01:10:46 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline (PGAP) Annotation Method :: Best-placed reference protein set; GeneMarkS-2+ Annotation Software revision :: 6.7 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total) :: 2,086 CDSs (total) :: 2,002 Genes (coding) :: 1,930 CDSs (with protein) :: 1,930 Genes (RNA) :: 84 rRNAs :: 5, 5, 5 (5S, 16S, 23S) complete rRNAs :: 5, 5, 5 (5S, 16S, 23S) tRNAs :: 65 ncRNAs :: 4 Pseudo Genes (total) :: 72 CDSs (without protein) :: 72 Pseudo Genes (ambiguous residues) :: 0 of 72 Pseudo Genes (frameshifted) :: 41 of 72 Pseudo Genes (incomplete) :: 35 of 72 Pseudo Genes (internal stop) :: 29 of 72 Pseudo Genes (multiple problems) :: 25 of 72 CRISPR Arrays :: 1 ##Genome-Annotation-Data-END## ##antiSMASH-Data-START## Version :: 8.dev-cf2fc5ee(changed) Run date :: 2025-09-12 16:47:59 NOTE :: This is a single region extracted from a larger record! Orig. start :: 625899 Orig. end :: 648398 ##antiSMASH-Data-END## REFSEQ INFORMATION: The reference sequence is identical to CP023477.1. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ Bacteria and source DNA available from Dr. Jacqueline Abranches. COMPLETENESS: full length. FEATURES Location/Qualifiers region 1..22499 /candidate_cluster_numbers="1" /contig_edge="False" /product="RaS-RiPP" /product="RRE-containing" /region_number="3" /rules="(PF04055 and TIGR01716)" /rules="minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])" /tool="antismash" cand_cluster 1..22499 /candidate_cluster_number="1" /contig_edge="False" /detection_rules="(PF04055 and TIGR01716)" /detection_rules="minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])" /kind="interleaved" /product="RaS-RiPP" /product="RRE-containing" /protoclusters="1" /protoclusters="2" /tool="antismash" protocluster 1..22179 /aStool="rule-based-clusters" /category="RiPP" /contig_edge="False" /core_location="[10000:12179](+)" /cutoff="5000" /detection_rule="(PF04055 and TIGR01716)" /neighbourhood="10000" /product="RaS-RiPP" /protocluster_number="1" /tool="antismash" proto_core 10001..12179 /aStool="rule-based-clusters" /tool="antismash" /cutoff="5000" /detection_rule="(PF04055 and TIGR01716)" /neighbourhood="10000" /product="RaS-RiPP" /protocluster_number="1" misc_feature 64..73 /note="TFBS match to AbrC3, Antibiotic production activator, confidence: strong, score: 16.78" /tool="antismash" gene 153..956 /locus_tag="CO204_RS03365" /old_locus_tag="CO204_03355" CDS 153..956 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_019804161.1" /locus_tag="CO204_RS03365" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CO204_03355" /product="MBL fold metallo-hydrolase" /protein_id="WP_002262928.1" /transl_table=11 /translation="MTETGFRYSILASGSSGNSFYLETPQKRILIDAGLSGKKISQLMA EIDRKVDDVDAILVTHEHKDHIHGVGVLARKYGLDVYANAATWQAMDGMIGKIDVSQKH IFDLGKTITFGDIDIESFGVSHDAAQPQFYRLMKDGKSFVILTDTGYVSDRMADLIENA DGYLIESNHDIEILRSGIYPWSLKQRILSDKGHLSNDDGAETMVRTLGNKTKKIYLGHL SKENNVKELAHMTMKNALMQADLAVDHDFKIYDTSPDTALPLAKI" misc_feature complement(162..180) /note="TFBS match to LexA_variant_3, Repressor of DNA damage response, confidence: medium, score: 24.33" /tool="antismash" gene 1063..1758 /gene="rnc" /locus_tag="CO204_RS03370" /old_locus_tag="CO204_03360" CDS 1063..1758 /EC_number="3.1.26.3" /GO_function="GO:0003723 - RNA binding [Evidence IEA]" /GO_function="GO:0004525 - ribonuclease III activity [Evidence IEA]" /GO_process="GO:0006364 - rRNA processing [Evidence IEA]" /GO_process="GO:0006396 - RNA processing [Evidence IEA]" /codon_start=1 /gene="rnc" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_019322985.1" /locus_tag="CO204_RS03370" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CO204_03360" /product="ribonuclease III" /protein_id="WP_002266141.1" /transl_table=11 /translation="MKTLEKKLAEDFKIVFSDKELLETAFTHTSYANEHRLLNISHNER LEFLGDAVLQLTISRYLFDKYPQKAEGDLSKMRSMIVREESLAGFSRNCHFDRYIKLGK GEEKSGGRQRDTILGDLFEAFLGALLLDAGLKAVEAFLNQVVIPKVENNNYERVTDYKT ALQELLQVDGDVLIDYEVLKESGPAHAKCFEVAVSMNHEKLSSGTGKSKKLAEQEAAKN ALEKLQRGS" gene 1758..5294 /gene="smc" /locus_tag="CO204_RS03375" /old_locus_tag="CO204_03365" CDS 1758..5294 /GO_function="GO:0005524 - ATP binding [Evidence IEA]" /GO_process="GO:0007059 - chromosome segregation [Evidence IEA]" /GO_process="GO:0030261 - chromosome condensation [Evidence IEA]" /codon_start=1 /gene="smc" /gene_functions="other (smcogs) SMCOG1218: Exonuclease" /gene_kind="other" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_002266942.1" /locus_tag="CO204_RS03375" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CO204_03365" /product="chromosome segregation protein SMC" /protein_id="WP_019313341.1" /transl_table=11 /translation="MFLKEIEMQGFKSFADKTKVEFDRGVTAVVGPNGSGKSNITESLR WALGESSAKSLRGGKMPDVIFAGTENRKPLNYAQVTVILDNSDAFIKDAQEEIRIERHI YRNGDSDYLIDGKKVRLRDIHDLFMDTGLGRDSFSIISQGRVEEIFNSKPEERRSIFEE AAGVLKYKTRKKETQSKLTQTQDNLDRLDDIIYELETQIKPLERQAKTAKEFLVLETER KEKQLDLLVYQILHHKEALVKNQAELEKVKQNLAAYYQERDLLETKNQTLKEKRHQLSR QMDQKQADLLEMTRLISDYERQIERIHLEVSQKTEKKQSTQKSLEQLSEQKASLKAELA QKEVDLEQLEEDLKQKNQEIKNVETELSRFATDPDHIIESLREDFVRLMQKEADTSNQL AVLKAEMDSRKQESESKTAEIKQVQADLEKAKDREQRESANFETAKTKVQELLKDYQKT AQLVQNLEAAYAEQQEAMFQLLDDVKDKKARQSSLKSILKSHSNFYAGVRSVLQQADKI KGIVGAVSEHLTFDKKYQTALEIALGASSQNIIVEDEAAAKRSIDFLKKNRQGRATFLP LTTIKPRRLSESNQSLLTSSQGFLGLARDLVSFEPRLQTIFGNLLGVTAIFDTVGHANQ AARQLRYQVRLVTLDGTEIRPGGSFSGGTSRQNNTTFIKPELDHLTQELALLEEKQVEQ ERTVEKTKQDLEVKKADLLELREKGNQARLAEQKAEMEYQQSESHLQELVLLYTQLKQT NQADLNRDLEKDQALLQEKLYKIADDKEKLNQEIAQIKEDKDSIQQKTAALSQQLSSLQ LSERDLSNTQKFERTNLKRLAEELAELEQNEVGMMQLLDSQEEDLDEKRLPSLKQQLAN AQARKIESDQELVRYRFELEDCEAQLEEVETNLLKTNQKNEEFIRQQTQLEAKGDHLSN QLRRFANNLAEDYQLSLDDAKQKAGPIENLEAAQAHLSQLEKQIKGLGPVNLDAIGQYD EVSERLHFLNSQKDDLVHAKELLLNTIHDMDDEVKARFKTTFEAIRDSFKTTFTQMFGG GSADLLLTEGDLLMTGVEISVQPPGKKIQSLNLMSGGEKALSALALLFAIIRVKTIPFV ILDEVEAALDEANVKRFGDYLNRFDKSSQFIVVTHRKGTMSAADSIYGVTMQESGVSKI ISVKLKEVEKIS" protocluster 2176..22499 /aStool="rule-based-clusters" /category="RiPP" /contig_edge="False" /core_location="[12175:12499](+)" /cutoff="10000" /detection_rule="minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])" /neighbourhood="10000" /product="RRE-containing" /protocluster_number="2" /tool="antismash" proto_core 12176..12499 /aStool="rule-based-clusters" /tool="antismash" /cutoff="10000" /detection_rule="minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])" /neighbourhood="10000" /product="RRE-containing" /protocluster_number="2" gene 5734..6777 /gene="pheS" /locus_tag="CO204_RS03380" /old_locus_tag="CO204_03370" CDS 5734..6777 /EC_number="6.1.1.20" /GO_component="GO:0005737 - cytoplasm [Evidence IEA]" /GO_function="GO:0000166 - nucleotide binding [Evidence IEA]" /GO_function="GO:0004826 - phenylalanine-tRNA ligase activity [Evidence IEA]" /GO_function="GO:0005524 - ATP binding [Evidence IEA]" /GO_process="GO:0006432 - phenylalanyl-tRNA aminoacylation [Evidence IEA]" /codon_start=1 /gene="pheS" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_019314825.1" /locus_tag="CO204_RS03380" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CO204_03370" /product="phenylalanine--tRNA ligase subunit alpha" /protein_id="WP_002268116.1" /transl_table=11 /translation="MDLQAQLEELRKSTQATLKEMSGNHSKELQDLRVKVLGKKGSLTE LLKGLKDLPNELRPVVGKQVNEVRDVLTKAFEEQAKIVEAAKIQAQLESETLDVTLPGR QIHLGNRHVLSQISEEIEDIFLGMGFQVVDGYEVEQDYYNFERMNLPKDHPARDMQDTF YISEDILLRTHTSPVQARTLDKHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQIEGLV VGKNISMGDLKGTLEMISKKMFGEDRKIRLRPSYFPFTEPSVEVDVSCFKCGGKGCNVC KKTGWIEILGAGMVHPSVLEMSGVDSEEYSGFAFGLGQERMAMLRYGINDIRGFYQGDS RFTEQFS" misc_feature complement(6844..6856) /note="TFBS match to MatP, Cell cycle regulator, confidence: weak, score: 22.29" /tool="antismash" gene 6850..7371 /locus_tag="CO204_RS03385" /old_locus_tag="CO204_03375" CDS 6850..7371 /EC_number="2.3.1.-" /GO_function="GO:0008080 - N-acetyltransferase activity [Evidence IEA]" /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_019317018.1" /locus_tag="CO204_RS03385" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CO204_03375" /product="N-acetyltransferase" /protein_id="WP_019313340.1" /transl_table=11 /translation="MSQVEIRKVNQDELSLLQKIAIQTFHETFAFDNTAEQLQNFFDEA YTLSALKLELADKESETYFILMSGKAAGFLKVNWGSSQTEQVLEDAFEIQRLYILKAYQ GLGLGKQLFEFALERAQISGLSWVWLGVWEKNVKAQLLYAKYGFEQFSKHSFFVGNKVD TDWLLKKSLS" gene 7385..9790 /gene="pheT" /locus_tag="CO204_RS03390" /old_locus_tag="CO204_03380" CDS 7385..9790 /EC_number="6.1.1.20" /GO_function="GO:0000287 - magnesium ion binding [Evidence IEA]" /GO_function="GO:0004826 - phenylalanine-tRNA ligase activity [Evidence IEA]" /GO_function="GO:0005524 - ATP binding [Evidence IEA]" /GO_process="GO:0006432 - phenylalanyl-tRNA aminoacylation [Evidence IEA]" /codon_start=1 /gene="pheT" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_002311998.1" /locus_tag="CO204_RS03390" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CO204_03380" /product="phenylalanine--tRNA ligase subunit beta" /protein_id="WP_106006701.1" /transl_table=11 /translation="MLVSYKWLKELVDIDVPSHELAEKMSTTGIEVEGVDVPAEGLSKL VVGHVLSCEDVPETHLHLCQVDTGEEESRQIVCGAPNITAGIKVIVALPGARIADNYKI KKGKIRGMESLGMICSLQELGLPDSIIPKEYSDGIQILPEDAVPGESIFPYLDLDDEII ELSITPNRADALSMRGVAHEVAAIYDKTVHFEDKVLTESDKKAADLIKVAIASDKVLTY KARVVENVTIKPSPQWLQNLLMNAGIRPINNVVDVTNYVLLYFGQPMHAFDLDKFEGKD ILARQAKAGEKLVTLDGEARDLIEEDLVITVADKPVALAGVMGGAATGIDASSKNVVLE AAVFDGKSVRKTSSRLNLRSESSSRFEKGVNYADVVKALDFAAAMLTELADGTVLAGQV SAGAVPTDDIQVSTSLDYVNVRLGTDLVFSDIESVFARLGFGLSGNDEKFTVSVPRRRW DISIQADLVEEIARIYGYDKLPTTLPEAAGTVGELTKSQKLRRKIRSLAEGSGLSEIIS YALTTPEKAVAFTPNPSKVTELMWPMTVERSALRQNIVSGMLDTIAYNVARKSNNLALY EIGKVFEQTADPKKDLPKEIDTFAFALTGLVTEKDFQTPATPVDFFYAKGVLEAILAKL EITVQFVATKEMANMHPGRTALIERDGQVIGYLGQVHPQTAKLYDIPETYVAELNLNAL EAALKSDLVFEDITKFPAVSRDIALLLSEKVSHQDILDAIAASGVKRLTKVKLFDVYAG EKLGQGKKSMAYSLTFQNPNDNLTDEEVAKYMEKITSALTEKVGAEVR" gene 10001..10858 /locus_tag="CO204_RS03395" /old_locus_tag="CO204_03385" CDS 10001..10858 /codon_start=1 /gene_functions="biosynthetic (rule-based-clusters) RaS-RiPP: TIGR01716" /gene_kind="biosynthetic" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_019804849.1" /locus_tag="CO204_RS03395" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CO204_03385" /product="Rgg/GadR/MutR family transcriptional regulator" /protein_id="WP_002267478.1" /sec_met_domain="TIGR01716 (E-value: 3e-48, bitscore: 161.2, seeds: 14, tool: rule-based-clusters)" /transl_table=11 /translation="MTEYGKIFKKFRESRGLKMKEVAGSNLSTSHLSRFENGESELTIS KLMTALYEINMPIEEFMYAANDFHQDELNELLKDVRECVNNKDVERLKAVLIKQQENLK EGRIFHHLNVILVKIRLQDLSGETYYSKADLDYLTNYLFKVEYWGFYELLLFTNTIDVF KHETFMVLSREMMRRSDFYKEIPRNRRLISRMALNSFITCIERDKLIDALYFEKQLSIC HFDETEIYERLVFHYAQNLFAYKKYKTTFAIIEMKKSVAAMKLAGSLHIAETYENHLQK ILEG" misc_feature complement(10906..10919) /note="TFBS match to PerR, Peroxide regulator, confidence: medium, score: 26.23" /tool="antismash" gene 11064..12179 /locus_tag="CO204_RS03400" /old_locus_tag="CO204_03390" CDS 11064..12179 /GO_function="GO:0003824 - catalytic activity [Evidence IEA]" /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding [Evidence IEA]" /GO_function="GO:1904047 - S-adenosyl-L-methionine binding [Evidence IEA]" /codon_start=1 /gene_functions="biosynthetic (rule-based-clusters) RaS-RiPP: PF04055" /gene_functions="biosynthetic-additional (rule-based-clusters) PTHR43273" /gene_functions="biosynthetic-additional (rule-based-clusters) Fer4_12" /gene_functions="biosynthetic-additional (rule-based-clusters) QueE" /gene_functions="biosynthetic-additional (rule-based-clusters) TIGR04085" /gene_functions="biosynthetic-additional (rule-based-clusters) SPASM" /gene_kind="biosynthetic" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_002265228.1" /locus_tag="CO204_RS03400" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CO204_03390" /product="radical SAM protein" /protein_id="WP_019313337.1" /sec_met_domain="PF04055 (E-value: 7.7e-22, bitscore: 75.3, seeds: 518, tool: rule-based-clusters)" /sec_met_domain="PTHR43273 (E-value: 5.2e-47, bitscore: 157.5, seeds: 35, tool: rule-based-clusters)" /sec_met_domain="Fer4_12 (E-value: 5e-11, bitscore: 40.0, seeds: 84, tool: rule-based-clusters)" /sec_met_domain="QueE (E-value: 1.2e-08, bitscore: 31.6, seeds: 32, tool: rule-based-clusters)" /sec_met_domain="TIGR04085 (E-value: 9.4e-13, bitscore: 45.3, seeds: 281, tool: rule-based-clusters)" /sec_met_domain="SPASM (E-value: 3.5e-11, bitscore: 40.4, seeds: 166, tool: rule-based-clusters)" /transl_table=11 /translation="MRDYSPYPLLVDVCVTNRCNLSCSYCSAEAGPFASKKGEMSVEKL DSIFRELNLMGVPRVGVTGGEPFIRDDILDILKAFDKYHFAKVLNTNGNLITDSVAKEL SKLHLDRICVTVDGSCSKVHDSQRGKGSFKKAIEGIKNLQRYNLPVSTLFTLGRHNVDD LINTIRLNDMLRIKYMSVMIICPTGRANDGSILADKKHWYPVFLDLTERLSRGEFKVKF KIVPPNESDVFWTHYFPLAFYNRLDLLESVWHQSLNFNSNKKREISCQAGVRACSINHK GDVYGCDLMNGIDELVAGNVKEKSFYEIWHNSPIFHKLRNITFNDISGKCADCPLPWCG GGCRCSALELDGTLLGSDLACFYDEEESVLV" gene 12176..12499 /locus_tag="CO204_RS03405" /old_locus_tag="CO204_03395" CDS 12176..12499 /codon_start=1 /gene_functions="biosynthetic (rule-based-clusters) RRE-containing: Stand_Alone_Lasso_RRE" /gene_functions="biosynthetic (rule-based-clusters) RRE-containing: Streptide_RRE" /gene_functions="biosynthetic-additional (rule-based-clusters) PF05402" /gene_kind="biosynthetic" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_002265227.1" /locus_tag="CO204_RS03405" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CO204_03395" /product="PqqD family protein" /protein_id="WP_002281716.1" /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 4.2e-09, bitscore: 33.2, seeds: 25, tool: rule-based-clusters)" /sec_met_domain="Streptide_RRE (E-value: 6.8e-39, bitscore: 129.2, seeds: 8, tool: rule-based-clusters)" /sec_met_domain="PF05402 (E-value: 1.8e-07, bitscore: 28.2, seeds: 461, tool: rule-based-clusters)" /transl_table=11 /translation="MKNHFTIKGKRDFVVDKIDDEFIGYDRLDLEYYSFDDIGAEILYC ISKNFSLNKIIEVIQEEYDVTYDDCKKAIVHFLEETPILHIIYANLIKSDIYLYLKPFR EAL" aSDomain 12191..12412 /aSDomain="Stand_Alone_Lasso_RRE" /aSTool="RREfinder" /database="RREFam.hmm" /description="Stand-alone RRE protein in a lasso peptide cluster" /detection="hmmscan" /domain_id="rrefinder_CO204_RS03405_Stand_Alone_Lasso_RRE.1 " /evalue="5.90E-11" /identifier="RREFam002.1" /label="CO204_RS03405" /locus_tag="CO204_RS03405" /protein_end="79" /protein_start="5" /score="33.2" /tool="antismash" /translation="TIKGKRDFVVDKIDDEFIGYDRLDLEYYSFDDIGAEILYCISKNF SLNKIIEVIQEEYDVTYDDCKKAIVHFLE" aSDomain 12191..12490 /aSDomain="Streptide_RRE" /aSTool="RREfinder" /database="RREFam.hmm" /description="RRE-containing rSAM enzyme in a streptide-like cluster" /detection="hmmscan" /domain_id="rrefinder_CO204_RS03405_Streptide_RRE.1" /evalue="9.60E-41" /identifier="RREFam022.1" /label="CO204_RS03405" /locus_tag="CO204_RS03405" /protein_end="105" /protein_start="5" /score="129.2" /tool="antismash" /translation="TIKGKRDFVVDKIDDEFIGYDRLDLEYYSFDDIGAEILYCISKNF SLNKIIEVIQEEYDVTYDDCKKAIVHFLEETPILHIIYANLIKSDIYLYLKPFRE" gene 12496..13776 /locus_tag="CO204_RS03410" /old_locus_tag="CO204_03400" CDS 12496..13776 /GO_component="GO:0016020 - membrane [Evidence IEA]" /GO_function="GO:0015297 - antiporter activity [Evidence IEA]" /GO_function="GO:0042910 - xenobiotic transmembrane transporter activity [Evidence IEA]" /GO_process="GO:0055085 - transmembrane transport [Evidence IEA]" /codon_start=1 /gene_functions="transport (smcogs) SMCOG1086: MATE efflux family protein" /gene_kind="transport" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_019803472.1" /locus_tag="CO204_RS03410" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CO204_03400" /product="MATE family efflux transporter" /protein_id="WP_002265226.1" /transl_table=11 /translation="MSYSKEVNRYVFPLVATNLVQVLIGQISLHFAVDNSNAALSGISV IQNFLFAFGGILGAFSLSFNIRGAKAFYNKEYQRFKNLLKSSLLIDIGIGLSFILFCLG LSRPFLKVFYGFDGQLLELSTTYFKIMSPYILLTLLIFLFSNLLKIEKKTKPIFWIGTL SSVLDVLLNYYLVPLLGIRGAAISSILSLLVIVLGYFIFVKSLVFQALQEASTSKLELI RFGIPLSIQEILESVLFIMAFDAFMGRQGLRILSIYAVVSQLFAIVRTPAFMYAGSIAI FLPEARKKEDEKALIATIFRNSYAVIFIFACLVTVFSNPFAHFLSDEVIDNVLPFTLYT VVVMGATPLYESSKMLLQSNDDEQWVLKITSIVNILSILILLVLQLMGKQTYQSLYGIY GLSLIVLSILFIKRNSEKRVSSRKMTM" misc_feature complement(13950..13965) /note="TFBS match to RutR, Regulator of the pyrimidine and purine metabolism, confidence: weak, score: 20.06" /tool="antismash" gene <14091..14261 /locus_tag="CO204_RS03415" /old_locus_tag="CO204_03405" /pseudo="" CDS <14091..14261 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_002998015.1" /locus_tag="CO204_RS03415" /note="incomplete; partial in the middle of a contig; missing N-terminus; Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CO204_03405" /product="phenylalanine--tRNA ligase subunit beta" /pseudo="" /transl_table=11 /translation="KLFDVYAGEKLGQGKKSMAYSLTFQNPNDNLTDEEVAKYMEKITK ALTEKIGAEVR" gene 14625..14951 /locus_tag="CO204_RS03420" /old_locus_tag="CO204_03410" CDS 14625..14951 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_019803471.1" /locus_tag="CO204_RS03420" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CO204_03410" /product="hypothetical protein" /protein_id="WP_002262922.1" /transl_table=11 /translation="MTHVVSEIFKDGPVDRFGLRGDPVFWNYLEKHFQKVAMPYSERKL EEDIRTIYQDLTGKEFRMGEMALVKDFISESGGMSSGWLSDDFWLKSAIPLLKKRLAKW NETL" gene 15126..15371 /locus_tag="CO204_RS03425" /old_locus_tag="CO204_03415" CDS 15126..15371 /GO_component="GO:0016020 - membrane [Evidence IEA]" /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_004261618.1" /locus_tag="CO204_RS03425" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CO204_03415" /product="SemiSWEET family transporter" /protein_id="WP_002262921.1" /transl_table=11 /translation="MLILGWVATFMSIMMYVSYIPQIMNNLAGNKGDFIQPSVAALNCT LWVIYGLCKEKRDLPLAAANMPGIVFGLIAAITALV" misc_feature 16140..16153 /note="TFBS match to PerR, Peroxide regulator, confidence: weak, score: 22.82" /tool="antismash" gene 16239..19481 /gene="rexB" /locus_tag="CO204_RS03430" /old_locus_tag="CO204_03420" CDS 16239..19481 /GO_function="GO:0004518 - nuclease activity [Evidence IEA]" /GO_process="GO:0000724 - double-strand break repair via homologous recombination [Evidence IEA]" /codon_start=1 /gene="rexB" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_012997660.1" /locus_tag="CO204_RS03430" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CO204_03420" /product="ATP-dependent nuclease subunit B" /protein_id="WP_106006702.1" /transl_table=11 /translation="MKLLYTDIPYNITEILAEEAQIAAKAGKRVFYIAPNSLSFEKERA VLETLPERASFAITITRFEQMARYFVLNDIHQGETIDDNGLAMVFYRALSSFSDQDLRV FGRLKQDAHFINQLVDLYKELKASNLTVLELNQLNSAEKQEDLIKIFTEVETILSAGHF DNQSKIAFFAQQIKFGHLDTALQDLTIVIDGFTRFSAEEENLIGLLYEKGVDIIIGTYI SQKAYRSTFSNGNVYQASLDFIRGLAGKFQTKPEYVVSKQEALPAFTKLSQLFESRHDF SDSQLVLTDKDKEHVTIWDVINQKEEVEHVAKSIRRKLYEGHRYKDILVLLGDVDAYKL QIGKIFDKYEIPYYFGKAESMSSHPLVHFVDSLERVKRYNFRAEDVMNLLKSGLYGKIR QNQLDKLEQYVTYADIKGKTKFFKDFTLDNHGQFDLKALNKLRAEVMSPLQELVKIQAQ KGDSILQKLTNFLEAISLTNNFSKLIQGVSDTEQEKNEQVWKTFTVILEQFHTIFGQEK MKLADFLALLRSGMLAADYRTVPASVDVVTVKSYDLVEPHSNKFVFALGMTQSHFPKIV QNKSLISDEERAKINEATPDNRRFVIVTKENLKKNHFTALSLFNAATQELVLTLPQILN EAEDNTSSYLLELQDMGVPVVEKGRNRLAADPEDISNYKALLSRVIELNRSAIDQELSK EEQTFWSVAVRYLRQKLAKEGLTIPEVNDKMQTKQVAAEVMAARFPIDQPLNLSSSALT TFYNNQYLYFLQYILGLQELETIHPDARNHGTYLHRVFELVMQDQSTDDFDSKLNRAID ITNHEDSFHLVYNEDEESRYALGILEDIARSTATILKGDNPVQAESEEEAFELMLDQAV KIRGVIDRIDRLSDGSLGIVDYKSSKNTFDLQKFYNGLSPQLVTYIEALRSCKNLNETD KIFGAMYLHMQEPKTDLANMKSIEKIPEIVHKNLSYKGLFLEDEKAHLANGKYHLHDAV YSQKEVDLLLDYNKRLYRSAAKQIRKGNFLINPYSQDGKSVQGEQLKAITHFEADRHMP YARKLYQLPRKEKRQGFLALMQSKKEEEGNDL" misc_feature complement(20390..20400) /note="TFBS match to HypR, L-hydroxyproline utilization repressor, confidence: weak, score: 18.94" /tool="antismash" misc_feature 22290..22304 /note="TFBS match to ZuR_variant_1, Zinc-responsive repressor, confidence: medium, score: 23.01" /tool="antismash" misc_feature 22482..22497 /note="TFBS match to RutR, Regulator of the pyrimidine and purine metabolism, confidence: weak, score: 20.01" /tool="antismash" ORIGIN 1 ggattagcaa tcgccaaaga aattgtcaaa cagcataaag gctttatttg ggccaatagt 61 gaggaaggcg aagggtcgac ctttaccatc gttttacctt atgaaaacga taatgatgca 121 attgatgaat gggaagaaga tgaagacgaa tcatgacaga aacaggtttt agatacagca 181 ttctggcttc tggttcaagt ggcaatagtt tttatttgga aactcctcaa aagagaattt 241 taattgacgc aggcctctca ggtaagaaaa ttagtcaatt aatggcagaa atagaccgaa 301 aagtagatga tgttgatgcc attttagtaa cacatgaaca taaagatcat attcacggtg 361 ttggtgtact agctcggaaa tatggtttgg atgtctatgc gaatgcagca acatggcaag 421 ctatggatgg catgattggt aaaattgatg tcagccaaaa acatatcttt gatctgggga 481 aaaccatcac ttttggggat attgatattg aaagttttgg tgtcagccat gatgctgctc 541 aaccacagtt ttaccgcctg atgaaagacg gcaagagttt tgtcatattg acggatacag 601 gttatgtcag tgatcgtatg gcagatctga ttgaaaatgc tgatggttat ctgattgagt 661 ccaatcatga tattgaaatt ttacgctcag gtatctaccc ttggagttta aaacagcgta 721 ttttatccga taaaggacac ctatcaaatg atgatggtgc agaaacaatg gttcgaactt 781 tggggaataa aacaaaaaag atttatttgg gacatctcag caaagaaaac aatgtcaaag 841 aattagccca tatgacgatg aagaatgctc tcatgcaggc agatttggct gtagatcatg 901 attttaaaat ttatgatacc tcgccagata ctgctttacc attagcaaaa atctagtgga 961 cagcttaaaa ggaagaatga aaaacaaaga ttactcggaa atggttgttc tgggtgattt 1021 ttgctataat agaaaggtct aaggataaaa gaaaggattt atatgaaaac attagaaaaa 1081 aaactggcag aagactttaa gatcgtcttt tctgacaagg aattattgga aactgccttt 1141 actcatacta gttatgctaa tgagcatcgc ctcctaaaca tttcacataa cgagcgcttg 1201 gaatttttag gagacgctgt tctgcagtta acgatttcac gttatctttt tgacaaatac 1261 cctcaaaaag ctgaaggtga tttatcaaaa atgcgttcga tgattgttcg tgaagaaagt 1321 ttggcgggtt tttctagaaa ttgtcacttt gaccgctata ttaaattagg taagggtgaa 1381 gaaaaatctg gcggtcgtca aagagatact attttgggcg atttatttga agctttttta 1441 ggtgctcttt tattggatgc aggtcttaaa gctgttgagg cctttctcaa tcaagttgtg 1501 attcctaagg ttgaaaataa taattatgaa cgtgtgaccg attataaaac ggcacttcaa 1561 gaattattgc aagttgacgg ggatgttttg atcgattacg aggtcttaaa agaatcagga 1621 cccgcccatg caaaatgctt tgaagttgct gtttctatga atcatgaaaa acttagtagc 1681 gggacaggca aatcaaaaaa attagcagag caagaggctg ctaaaaatgc gcttgaaaaa 1741 cttcaacgag gttcttaatg tttttaaaag aaattgaaat gcagggattt aaatcttttg 1801 ctgataagac taaggttgag ttcgacagag gagtgacagc cgttgttgga ccaaatggtt 1861 caggaaagag taatattacg gagagtttac gctgggcttt gggtgagtca agtgccaaga 1921 gcctgcgtgg cggcaagatg ccagacgtga tttttgcagg aactgaaaat cgaaagccac 1981 tcaattacgc tcaagtaacg gttattttag ataatagcga tgcttttatc aaagacgctc 2041 aagaggagat tcgcattgaa cgccatattt atcgtaatgg ggacagcgat tatcttattg 2101 atggtaaaaa agtacgcctg cgcgatattc acgatttgtt tatggatact ggtttggggc 2161 gtgattcctt ttccattatt tctcaaggac gtgtggaaga gatttttaat agtaaaccag 2221 aagagcgtcg ttcaattttt gaagaagcag ccggtgtctt aaaatacaaa acacgtaaaa 2281 aagagacgca gtctaagcta acacagactc aggataattt ggatcggctg gatgatatta 2341 tctatgaact ggagacacag ataaagcctt tagaacgtca ggcaaaaaca gccaaagaat 2401 ttctggtttt ggaaacagag cgcaaggaga aacagttaga cttgctggtc taccaaattc 2461 tccatcataa ggaagcctta gtgaaaaacc aagctgagct tgaaaaagtt aaacaaaatt 2521 tagcagccta ttatcaagaa cgtgatctat tagaaactaa gaatcagact ctaaaagaaa 2581 aaaggcacca gctttctcgt caaatggatc aaaagcaggc tgatctcctt gaaatgacac 2641 gtctcattag tgattatgag cgtcagattg aacgcattca tttagaggtt agtcaaaaga 2701 cagaaaagaa gcaatctact caaaaaagcc tagaacaatt gtcagagcaa aaggcttctc 2761 ttaaagcaga attagctcaa aaagaagttg acttagaaca attagaagag gatctcaaac 2821 aaaaaaatca agaaattaaa aatgtcgaaa cggaattgtc acgttttgca acagatcctg 2881 atcatattat tgaaagttta cgagaagatt ttgttagact catgcaaaaa gaggctgata 2941 cttctaacca attggctgtt ttaaaagcag aaatggatag tcgaaagcaa gaatccgaaa 3001 gtaaaacagc agaaattaaa caagtacaag cagacttaga gaaagcaaaa gatcgagaac 3061 aaagggaatc cgcaaacttt gaaactgcta aaaccaaagt tcaagaattg ctaaaggact 3121 atcaaaaaac agcacagctt gttcagaatt tagaagctgc ttatgctgag cagcaagagg 3181 ctatgtttca attgttggat gatgtcaaag acaagaaagc acgccaaagc agtctgaagt 3241 ccatcttaaa aagccatagt aatttctatg caggtgttag gtctgttctt cagcaggctg 3301 ataaaattaa aggtattgtt ggcgcggtta gtgaacattt aacttttgat aaaaaatatc 3361 aaaccgcact tgagattgcc ttaggtgcca gcagtcaaaa tattattgtt gaagatgaag 3421 cggcggccaa acgatctatt gattttttaa agaagaaccg tcaaggtcga gcaacttttc 3481 ttcctttaac aaccattaaa ccccgtcgtt tatctgagtc aaatcaaagc ctattaactt 3541 ccagccaggg ctttctcggt ttagcacgtg atttggtaag ttttgagcct cgtttgcaaa 3601 caatttttgg caatttactg ggtgtcacag cgatttttga tacggttggt catgccaatc 3661 aggcagctcg ccagttacgt tatcaagtgc gtcttgtgac attagatgga acagagatcc 3721 gtcccggagg gtccttttca ggagggacta gccgtcaaaa taatacaact tttatcaagc 3781 cagaactgga tcatttgaca caagagctag cgcttttaga agaaaaacaa gttgagcagg 3841 aaagaacagt tgaaaagacc aagcaggact tggaagtgaa aaaagcagat cttttagaat 3901 taagggaaaa aggaaatcaa gcccgattgg ctgagcaaaa ggctgagatg gagtatcaac 3961 aatcagagtc tcatttacaa gagctggtct tgctttatac ccaattaaaa caaacaaatc 4021 aggcggatct taatcgagac ttagaaaaag accaagcact tttacaagaa aagctgtata 4081 aaattgctga tgacaaggaa aagctcaatc aagagattgc tcaaatcaag gaagataagg 4141 atagtattca gcaaaagaca gctgctttaa gtcaacaact ctcttctttg caactttctg 4201 agcgtgattt gtctaataca cagaaatttg aaagaacgaa tttaaaacgt ttggcagaag 4261 aattagctga attggagcaa aatgaagttg gtatgatgca acttttagat agccaagaag 4321 aggatttgga tgaaaaacgt ttaccatcgc tcaaacagca attagcaaac gctcaggcta 4381 gaaaaataga gagtgaccaa gaacttgtcc gctaccgttt tgaactggaa gactgtgaag 4441 cacagctaga agaggttgag accaatctcc taaaaactaa tcaaaagaat gaagagttca 4501 ttcgtcagca aacgcaatta gaagcaaaag gggatcattt atccaatcaa ttgcgccgtt 4561 ttgccaataa cttggctgaa gattatcaac taagcttaga tgatgctaaa caaaaggcag 4621 gaccgattga aaatctagaa gctgctcaag ctcatttaag ccaattggaa aagcaaatta 4681 agggattggg accggtcaac cttgacgcaa tcggtcagta cgatgaagtg tctgagcgtt 4741 tgcatttctt aaatagtcaa aaagatgacc ttgttcacgc taaggaactt ctcctgaata 4801 ctattcatga tatggatgat gaggtcaagg cacgctttaa gacgactttt gaagctattc 4861 gtgacagttt taagacgacc tttacgcaga tgtttggcgg aggaagtgct gacttgcttt 4921 tgacagaagg agatctcttg atgaccggtg ttgaaatttc agtgcagccg cccggcaaaa 4981 agattcaatc tctcaatctt atgtctggtg gtgaaaaggc actgtctgct ttggcattgt 5041 tatttgccat tattcgtgtt aaaaccattc cctttgttat tttggatgag gttgaggctg 5101 ctcttgacga ggccaatgtc aagcgttttg gtgattatct gaatcgtttt gataagtcca 5161 gccagtttat tgtggtcact caccgtaaag gcaccatgtc tgctgcagat agtatatatg 5221 gggttaccat gcaggaatca ggtgtctcta agattatctc tgttaagcta aaagaagtcg 5281 aaaagatatc ttaaaaaggc ttggaagtgc ttagtttgaa aaaactgtaa ggacacaatc 5341 aatctttgac ggcaatagta cttaaagtga ctctcaacaa ggaaacaagg cggtaacgac 5401 aaaatcattt ctgtaaattg caatggaatc tcgttctcca tagaatcttt tattcagcct 5461 attgagaaat tttgtgaaat ctgttaaaat agtaacaatt gaataaaagt ctatgagatt 5521 agtagcttta tggaagtttg acagggaatg cggggcgtga ctggaaaccc gctggatgaa 5581 agtttagtga attcactcag aaagaaaggc ttaataatta aggtctagtc atactagata 5641 aaaacggatg gtaccgcgtg tcaacgctcc gcttaaggag ttttggcacg ttttttgttt 5701 ttaacaaggt ccttaaatag gaaaggaaat cacatggatt tacaagcaca attagaagag 5761 ctgagaaaat caactcaagc aacattaaag gaaatgagcg gcaatcacag caaagaatta 5821 caagatttgc gtgttaaggt tttgggtaag aaaggttcct tgacagaact tttaaaaggt 5881 ctaaaggact taccaaatga gttgcgtcct gttgttggaa aacaagtcaa tgaagtccgt 5941 gatgtcttaa caaaggcttt tgaagaacaa gctaaaatcg ttgaagccgc caaaattcaa 6001 gcgcaattag aatcagaaac attagatgtc actcttccgg gacgccaaat acatttaggc 6061 aatcgccatg ttctcagtca gataagtgaa gaaattgaag atatattctt aggaatgggt 6121 tttcaagtgg ttgatggcta tgaagttgaa caagactatt acaatttcga acgcatgaat 6181 ttgcccaaag atcacccagc acgtgatatg caggacacgt tttatatttc tgaagacatt 6241 ttgcttcgaa cacataccag tcctgtgcag gcacgtactt tagataaaca tgatttttct 6301 aaagggccgc ttaagatgat ttcacccgga cgcgtttttc gccgcgatac agatgatgcg 6361 acgcattccc atcaattcca tcaaattgaa ggcttggtcg ttggtaaaaa catttctatg 6421 ggtgacctca aaggaacact ggaaatgatt agtaaaaaaa tgtttggtga agatcgtaag 6481 attcgtctgc gtccttctta tttcccattt acagagcctt ctgttgaagt ggatgtttcg 6541 tgttttaaat gcggcggcaa aggctgtaat gtttgtaaga aaacaggttg gattgaaatt 6601 cttggtgccg gtatggttca tccaagtgtt cttgagatgt ccggcgttga ctctgaagaa 6661 tattctggtt ttgcctttgg cttaggccaa gaacgtatgg ctatgctgcg ctatggaatc 6721 aatgatattc gaggtttcta ccaaggtgac agccgtttta cagaacagtt cagttaaatc 6781 tataaggttg tagagctttt tgcaagctaa ttgctctggc tcagccatcc acaagttaaa 6841 ggagtgaaaa tgtcacaggt tgaaattaga aaagtcaatc aagatgagct ttctttgctt 6901 cagaaaattg ctattcagac ttttcatgaa acttttgctt ttgataatac agcagagcag 6961 ctgcaaaact tttttgacga agcttatact ttgtctgctt taaaattaga attagctgat 7021 aaagaatcag aaacctattt tattttgatg tccggtaaag cagcgggatt tttgaaagtg 7081 aactggggca gctctcaaac cgagcaggta ctagaagatg catttgaaat tcaacgtctt 7141 tatattttaa aagcctatca aggtttaggt ttaggaaaac agctttttga atttgcttta 7201 gaacgtgcgc aaatatcagg cttatcatgg gtttggctgg gcgtttggga aaagaatgtt 7261 aaggcccagt tattgtatgc taaatatgga tttgaacaat tttctaagca ttcttttttt 7321 gttggtaaca aagtggatac ggattggttg cttaaaaagt ctttaagtta gggaaaggaa 7381 atacatgtta gtaagttata aatggttgaa agaattagtt gatattgatg tacctagtca 7441 cgaattggct gaaaaaatgt caacaacagg tattgaagtt gaaggggtag acgttccagc 7501 agaaggactg tcaaaattag tcgtcggtca tgtcctttct tgtgaagatg ttccagaaac 7561 gcatcttcat ctttgtcagg tggatactgg cgaggaagaa tctcgtcaaa tcgtttgtgg 7621 agcaccaaat attacagctg gcattaaagt aattgttgct cttccgggag ctcgtattgc 7681 agataattat aagattaaaa aagggaaaat ccgcggtatg gaatctctgg gtatgatttg 7741 ctctctgcag gagcttggtt tgccggacag cattattcct aaggagtatt cagatggcat 7801 tcaaattttg cctgaagatg ctgtgccggg tgaaagtatc ttcccttatt tggacttaga 7861 tgatgaaatc attgagctgt ctatcacacc taaccgtgca gatgctttat ccatgcgcgg 7921 tgtggctcat gaagtagcag cgatttatga taaaactgtt cattttgaag ataaagtctt 7981 gactgagagt gataagaaag cagctgacct tatcaaggta gccatcgctt ctgacaaggt 8041 tttgacttac aaagcgcgtg tggttgaaaa tgtaaccatt aaaccaagtc cacagtggct 8101 gcaaaatctg cttatgaatg ctggcattcg gcctattaat aatgtggttg atgtgaccaa 8161 ttatgttctt ctttattttg gacagcctat gcatgctttt gatttagata agtttgaagg 8221 caaggatatt cttgcacgtc aggcaaaagc tggtgagaaa ttagtaacac ttgatggtga 8281 agcgcgtgat ttgattgaag aggatcttgt cattactgta gcagataaac cagtggcact 8341 tgcaggtgtt atgggtggtg ctgcaactgg aattgatgcc tcttctaaaa atgttgtcct 8401 tgaagcagcc gtctttgatg gaaaatcagt tcgtaaaaca agcagccgtc ttaatctgcg 8461 ttccgaaagt tcttcacgtt ttgaaaaagg tgttaattac gctgatgttg ttaaggcact 8521 tgattttgct gcagcaatgc tgacggaatt ggcagatgga acagttctcg ctggtcaggt 8581 cagtgcagga gcagttccga cagatgatat tcaagtatcc actagtctgg attatgtcaa 8641 tgttcgctta ggtacagact tagttttctc agatattgaa agtgtttttg ctcgccttgg 8701 ttttggtctt tctggcaatg acgaaaaatt cacggtttct gtaccgcgtc gccgttggga 8761 tattagcatt caggcggatt tggtcgaaga aatcgctcgt atttatggtt atgataaatt 8821 accgacaaca ctgcctgaag cagcaggaac agttggtgaa ttgaccaagt cacaaaaatt 8881 acgtcgtaag ataagaagtt tggctgaagg tagtggtctt tcagaaatta tttcctatgc 8941 cctgacaacg cctgaaaagg cagttgcttt tacgccaaat cctagcaagg tgacagaact 9001 gatgtggcca atgaccgttg agcgttcggc actgcgtcaa aatattgtct ctggaatgct 9061 agataccatt gcctataatg tagcacgtaa gagcaacaat ctagccctat atgaaattgg 9121 caaagtattt gagcaaacag cggatcccaa aaaagatttg cctaaggaaa tagatacttt 9181 tgcctttgct ctgacaggtt tagtaactga aaaagatttc caaacaccag cgacaccagt 9241 tgatttcttc tatgctaaag gtgttctgga agctattctt gctaaacttg aaatcactgt 9301 tcaatttgtt gcgactaaag aaatggctaa tatgcatccc ggacgcacag ctttaattga 9361 acgtgatggt caagttatcg gttatcttgg tcaagttcat ccgcagactg ctaaactgta 9421 cgatattcca gaaacttatg tagcagaatt gaatcttaat gcactggaag cagctcttaa 9481 atcggattta gtctttgaag atatcactaa attcccagct gtttcgcgcg atattgccct 9541 gcttctttca gaaaaagtga gtcaccaaga tattttggat gctattgcag ccagcggtgt 9601 gaaacgcctt accaaagtaa aactctttga cgtttatgct ggtgaaaaat tgggacaagg 9661 taagaaatca atggcctaca gcctgacttt ccaaaatcct aatgataatc tcacagatga 9721 agaagttgcc aaatacatgg aaaaaatcac ttcagcccta accgaaaaag ttggtgcaga 9781 agtgaggtaa cgaaaaaatc taccttcaaa ggtagatttt tttggtatgt aagctagtga 9841 gcctacccac cgccgataat gataattgtt tctaatacca ctatcaaagt cataaaaatt 9901 ttatttctca tgtttttctc ctcactcact tttcttatat gttataataa attgcaatta 9961 actgaatata aatcccatat agacaattga ggtactattg atgactgaat atggaaaaat 10021 atttaaaaaa tttagagaat cacgcggtct aaaaatgaag gaagtagctg gttcaaattt 10081 atcaacttca catctttcaa gatttgaaaa tggagaatct gagctgacaa taagtaaact 10141 tatgacagca ctgtacgaaa taaatatgcc tattgaagaa tttatgtatg ctgccaatga 10201 tttccatcag gatgaattaa atgagctcct taaagatgtg agggagtgtg ttaataataa 10261 agatgttgaa cgattaaaag cggtattgat taagcaacaa gagaatttaa aagaaggtcg 10321 aatttttcac catttgaatg ttattctagt aaaaataagg ttgcaagatt tatccggtga 10381 aacttattat agtaaagcag acctagatta cttaacaaat tacttattta aagttgaata 10441 ctggggtttc tatgagctgt tactttttac gaatactatt gatgttttta aacatgaaac 10501 ttttatggtt ctctctagag agatgatgag acggtcagat ttttataaag aaatacctcg 10561 aaatcgacgt cttatttcac gtatggctct gaattctttt ataacttgca ttgaaagaga 10621 taaactgatt gatgcactgt attttgaaaa acaattaagc atctgtcatt ttgatgaaac 10681 agaaatttat gaacgcttgg tctttcatta tgctcaaaat ctatttgcct ataaaaaata 10741 taagacgaca tttgctatta ttgaaatgaa gaaatcagta gcagcaatga aattggcagg 10801 gagtttgcat attgctgaaa cctatgaaaa ccatcttcaa aaaattttag aaggataata 10861 tgaaattgtg tatatgggat aaaaatctct ctttttggat ttgatttata ataattctat 10921 caaatcaagg aggtaaatac tatgttgact aaaaaagagt ttagtgttcc taaaacaact 10981 aaggtaaact gttggggtaa gcactagtaa aacaaggtct gtaggtcatt gcatggctta 11041 cagatttttt aggaggtatt ttaatgagag attattcacc atatccgtta ttggttgatg 11101 tttgtgtaac aaatcgttgt aatttaagtt gttcatattg ttcagcagaa gcaggccctt 11161 ttgccagtaa aaaaggagag atgtctgttg agaagctgga ttctattttc cgtgaattaa 11221 atttaatggg agttccgcga gtaggtgtta ctggaggtga accgtttatt agagatgata 11281 tattagacat cttaaaggct tttgataaat atcattttgc taaagtttta aatactaatg 11341 gtaacctcat tacagattct gtagctaagg aattatctaa actacatttg gatagaattt 11401 gtgtgaccgt ggatggaagt tgctctaaag ttcatgatag ccaaagaggt aaaggaagct 11461 ttaaaaaggc aatagagggt attaaaaatt tacaaaggta taatcttcct gtttctacac 11521 tttttacatt aggaagacac aatgtagatg atttgattaa cactattagg ttaaacgata 11581 tgcttagaat taaatatatg tctgtaatga ttatttgccc aacaggtaga gctaatgatg 11641 gaagcattct agcagataag aaacattggt atcctgtatt tttagattta acagagagat 11701 tgtctcgtgg agaatttaaa gtgaagttta aaatagttcc tccaaatgaa agtgatgtct 11761 tttggacaca ttatttccca ctagcttttt acaatcgatt agatttactt gaaagtgtat 11821 ggcatcaaag cctgaacttt aatagtaaca agaaaagaga aatatcttgc caagctggag 11881 taagagcttg ttctatcaac cataaaggtg atgtttatgg ttgtgatttg atgaacggca 11941 ttgatgaact tgtagcaggt aatgtaaaag aaaaatcttt ttatgaaatt tggcataatt 12001 ctcctatatt tcataaattg agaaatatta catttaacga tatttcgggg aaatgtgctg 12061 attgcccctt accttggtgt ggtggtggat gtagatgcag tgcactggag ttagatggaa 12121 ctttgcttgg atctgattta gcatgttttt atgatgaaga ggaaagcgta ttggtatgaa 12181 aaatcatttt acaataaaag ggaagagaga ttttgttgtt gataaaattg acgatgagtt 12241 tataggatat gatagattag atctagagta ttattctttt gatgatatag gtgctgaaat 12301 tttatattgt atttctaaaa acttttcttt gaacaaaatc attgaggtca ttcaagaaga 12361 gtatgatgtg acttatgacg attgtaagaa agctattgtc cattttttag aggagacacc 12421 tatattacat attatttatg caaatcttat taaatcagac atttatttat acctaaaacc 12481 atttcgggag gcattatgag ttattctaaa gaggtgaacc gttatgtttt tcctctcgtt 12541 gccacaaacc ttgttcaagt tttgattggt caaatttctc ttcattttgc tgtcgataat 12601 tctaatgctg ccttgtctgg catttctgtt atccagaatt ttctttttgc atttggaggt 12661 attcttggag cctttagttt gtcgtttaat attagaggtg ccaaggcttt ttataataaa 12721 gaataccaac gatttaaaaa tcttttaaaa tcatccctgc taattgatat tggaattgga 12781 ctgtcattca ttcttttttg tctgggattg agcagacctt ttttgaaagt tttttatggt 12841 tttgatggtc aattattgga actatcgact acctatttca agatcatgtc tccttatatt 12901 ttattgacct tattaatctt ccttttttca aatcttttga aaattgagaa gaaaacaaag 12961 cccatttttt ggattggaac cctaagttcc gtgctagatg tcttgttaaa ctattactta 13021 gttcctcttt tgggaattcg tggagctgct atttcgtcta ttttatcgct gctagtcatt 13081 gttttagggt atttcatttt tgtaaaatct cttgtttttc aagcgcttca agaggcttcg 13141 acaagtaagt tagagcttat ccgttttgga attcctttat ccatacaaga aatactggag 13201 agtgttttat ttatcatggc tttcgacgct ttcatgggaa gacaggggct acgcattttg 13261 tccatctatg cggtagtcag tcaattattt gcaattgtga gaacacctgc ctttatgtat 13321 gcgggttcaa tagcaatctt tttgcctgaa gctaggaaaa aagaagacga aaaagcattg 13381 attgctacta tttttagaaa tagctacgct gttatattta tatttgcttg tttagtaaca 13441 gtattttcaa atccttttgc tcattttttg tctgatgaag ttattgataa tgtgctgcct 13501 tttactttgt acacggttgt ggtcatggga gcaacacctc tatatgaaag ctctaagatg 13561 cttttgcaaa gcaatgatga cgaacaatgg gttttaaaaa taacaagcat tgtcaatata 13621 ttaagtatcc ttatcttatt agttctccag ttaatgggca agcaaaccta tcaatctctg 13681 tatggcattt atggtttgag tttaatagtg cttagtattc ttttcataaa aagaaatagt 13741 gagaagagag tatcatcacg taaaatgaca atgtaaggct tgtagggagt aatacaagtc 13801 ttttttagaa ctttaatgaa taaaagtttt ttgacagttt acactagttt cacctaaaaa 13861 atattatcga tttttcaaac gaaacgtcgc agtaatcttt ggtggctaca ttaggaggtg 13921 atctaaggcc ttgaaaatcc ctttgaagat tgacaaatca gttaatcaat cttcttctgc 13981 ccacggcggt acctatagtt cttagtgacc ttacggtcgc tttcgataag ctaatgcttt 14041 aggcaataag taggaaatct atgaatatgg gttcttaata tcccaaagta aaactctttg 14101 acgtttatgc cggtgagaaa ttgggacaag gtaagaaatc aatggcctac agcctgactt 14161 tccaaaatcc taatgataat ctcacagatg aagaagttgc caaatacatg gaaaaaatca 14221 ccaaagccct aaccgaaaaa attggtgctg aagtgaggta aaaattgaca gctgataagc 14281 ttatagccgt ctgtttctta tttttaaaca gataacttag taaggacagt gcccatcaat 14341 gatgtttgct gttagttcaa tatgaaaaga aaattaattt ctgtaaacgt cgtttgtcaa 14401 gcctataaaa gacctgtgtt tgtgaaaaca atttcccatt ttaaagggat gatagtcatt 14461 tagtttatcc tttataattt tgttagcgca tcttaccata cgtttttgtg actaactttg 14521 tcagtttaat tatcaccctt caaccctccc agttgttaaa actgtctgtg gcttgctgcc 14581 ttgtcttaca atcaaaccaa aagactagaa taaggagaaa ggttatgaca catgttgtat 14641 ctgaaatttt taaagatggc ccggtagaca ggtttggttt acgaggggat cctgtttttt 14701 ggaactattt agaaaagcat tttcaaaagg tagctatgcc atattccgaa agaaaattag 14761 aagaggatat aagaacgatc tatcaagact taactggaaa agaatttaga atgggtgaga 14821 tggctcttgt taaagacttt ataagtgaat ccggcggcat gtcatcagga tggcttagtg 14881 atgatttttg gcttaaatca gctataccgc ttctgaaaaa acgattggca aaatggaatg 14941 aaactttata aagactgtaa tttattgatt atgaagaatg ataaatcaag caatggtgtc 15001 ataaaagtca aacgggatgg cttatcattc ttttgaaaac aatttgacat tttcaaagaa 15061 aagtttaaaa tggaaaacag tctgaagaaa tgaggtaatg aagaactttg cggaaagaaa 15121 aaaatatgtt gatattaggt tgggtagcga cttttatgtc tataatgatg tatgtgtctt 15181 atatccctca gatcatgaat aatttagcag gaaataaggg ggattttatc caaccgtcag 15241 tagctgcact taactgcaca ctgtgggtaa tttatggtct ttgtaaagaa aagcgagacc 15301 ttcctttagc tgccgccaat atgccaggca ttgtttttgg cttgattgca gcgattacgg 15361 cattagttta gctttataaa agcatttatt tcataaaggt tgtgaattga gtctgtgaaa 15421 tagagatttc tgtggaacgg ctcttttttt ctgtcattta tcaacctttt cctgagatga 15481 tacctactta atcacaatca aatagacgag gaaatgtgcc aaagttaatg agggctaagc 15541 aagaaaagct gttagcgacg gtctgtctag ccaccttgct agctttcttt ctcagtgtta 15601 ttgattgctc gaacgtgtat cgcaaggttt caagaaagtt cactgacgct ggattttcta 15661 cgaattttgg ataaaagtgt tctaactttt tattgtgtag tggttttagt ttaacgcagt 15721 gataaattgg aattttcgcg tcttgttttt taggctcaac cctttaaagt tcggtgttct 15781 gccggagctt atctaatcta ttttaaggaa agcaagacga caaattgaat cgagctttca 15841 ttacgattgg ccgaactttt tatacgtaat tttatatatt tacactattt tgtgaataat 15901 tgcgcagcaa tcagttttac ccgcacgagc gggggtgatc ccagtgacaa caacgaatca 15961 gatacagaaa tcatgtttta cctacacata tagacttgtg atgttagacc gattgaaatt 16021 gattcttttg gatagaagga aatcaaatat atatattaag tggatgacaa taaaagtaaa 16081 atattagata atagagatta agaattataa agaattggaa aatgtttagt cgtcaaacct 16141 aaaatcatta taatgatacg aatatttttt tgatcaattt tgtgataata taaagaagcg 16201 acatgcttac caattatttc aatctgatag aggtaataat gaaactttta tataccgaca 16261 ttccatacaa cataacagag attttggccg aggaagcaca aatagctgct aaggctggca 16321 agcgggtctt ttatattgct cccaactcct tgtcttttga gaaggagcga gctgttttgg 16381 agaccctgcc tgagcgggct tcctttgcta ttaccattac gcgttttgag caaatggcac 16441 gctattttgt tttaaatgat attcatcagg gcgaaaccat tgatgataat ggacttgcca 16501 tggtttttta tcgtgcttta tccagttttt cagaccaaga tttgcgtgtt tttgggcgtc 16561 ttaagcaaga tgctcatttt atcaatcaat tggtggattt gtataaggaa ctcaaggctt 16621 ctaatctgac tgtcttagaa ttaaatcagc taaattcggc tgaaaagcag gaagatttga 16681 ttaagatttt tacagaggtt gaaacaattt taagtgctgg ccattttgat aatcaaagca 16741 agattgcctt ctttgcacaa cagatcaaat tcggtcattt agatacggct ttgcaggatc 16801 tgacaattgt tattgatggt ttcacacgtt tttctgctga agaagaaaac ctgataggac 16861 tgttatatga aaaaggtgtt gacattatca ttgggactta tattagtcaa aaagcttatc 16921 gttcaacttt ttcgaatggg aatgtctatc aggctagcct tgatttcatt cgtggtttgg 16981 ctggtaaatt tcaaaccaaa ccagaatatg tggtatcgaa gcaagaagct ttgcctgcct 17041 ttaccaaact ttctcagcta tttgaaagtc gtcatgattt ttcggacagt cagctggtct 17101 tgacagataa ggacaaggaa catgtaacta tctgggatgt cattaatcaa aaagaagaag 17161 tggaacatgt tgctaagagc attcgccgga aactctatga aggtcatcgt tacaaggata 17221 ttttggtcct tcttggggat gtggatgcct ataaactgca gattggcaag atttttgata 17281 agtatgaaat tccctattat tttggcaaag cggagtccat gagttcgcac ccgctggttc 17341 attttgtgga ttctttggag cgggttaaac gttataattt tcgtgctgag gatgtcatga 17401 atctgctcaa atcaggccta tatggcaaaa tcaggcaaaa tcagttagat aaattggagc 17461 agtatgtcac ttatgctgat attaaaggaa aaacaaagtt ttttaaggac tttacgctag 17521 ataatcatgg gcaatttgat ctaaaagcct taaataaact gcgggcagaa gtcatgtcac 17581 ctttacaaga gctggttaaa atacaggccc aaaagggaga cagtatcctg caaaaattga 17641 ccaacttttt agaagccatt tctttaacga ataatttttc aaaattaatc caaggtgtca 17701 gtgacactga gcaggaaaag aacgagcaag tttggaaaac ttttacggtt attttagagc 17761 aatttcatac gatttttgga caagaaaaaa tgaagttggc tgattttctg gcactcttac 17821 gcagcggcat gctggcggct gattatcgga cggttcctgc cagtgttgat gttgtgacag 17881 ttaagtctta tgatttggtg gagccccata gcaataaatt tgtttttgct ctgggaatga 17941 cccagtctca ttttcctaaa attgtccaaa ataagagtct tatttcggat gaagaacgtg 18001 caaaaatcaa tgaagcaact cctgacaatc gtcgttttgt tattgtcact aaggaaaatc 18061 tcaagaaaaa tcatttcacc gccctgtcac tcttcaatgc ggccactcag gaattggtct 18121 taaccttgcc gcagatttta aacgaggcag aggacaacac ctcctcctat ttgcttgaat 18181 tgcaagatat gggtgtgcca gttgtcgaaa aggggcgcaa tcgtttagct gcggatccag 18241 aagatattag caattacaag gcgcttttgt cgcgtgtgat tgagcttaac cgctctgcta 18301 ttgaccaaga attatctaaa gaagagcaga ccttttggtc agttgctgtt cgctatttac 18361 gccaaaaatt ggcaaaagaa ggacttacga ttcctgaagt gaacgacaag atgcagacca 18421 agcaggtcgc tgcagaggtt atggcagcac gttttccaat agatcaacct ttaaatctat 18481 catcgtctgc cctgacaact ttttacaata atcagtatct ctatttcctt caatatatcc 18541 ttggtttgca agagcttgaa acgattcatc ctgatgctag aaatcatggg acttatttgc 18601 accgtgtgtt tgaattggtt atgcaagatc aatctactga tgattttgac agtaaattaa 18661 atcgtgccat tgatataacc aaccatgagg atagctttca cttggtttat aatgaagatg 18721 aggaaagtcg ctatgctttg ggtattttag aagatattgc tcgtagtacg gctaccattt 18781 taaaagggga taatcctgtt caggcagaaa gtgaagaaga agcctttgag ttaatgctgg 18841 atcaggctgt taaaatcaga ggggttattg atcgcattga tcgcttgtca gatggcagtt 18901 taggaattgt tgattataag tcaagtaaaa atacctttga tctgcaaaaa ttttacaatg 18961 gactcagtcc gcagttagtg acttatatag aagctcttcg gtcttgtaaa aatttgaatg 19021 agacagataa gatttttgga gccatgtatc ttcatatgca ggaacctaaa actgatttag 19081 ccaatatgaa atccatcgaa aaaattccgg aaatagttca taaaaattta agttataagg 19141 gactcttttt agaagatgaa aaagcacatt tggctaatgg gaaataccat ttgcatgatg 19201 ctgtctatag tcaaaaagaa gttgatttgc tgcttgatta taataaaaga ctctatcgca 19261 gtgccgcaaa gcaaatcaga aaaggaaact ttctcattaa cccctacagt caggatggca 19321 aatctgttca gggagaacag ctgaaggcta ttacacattt tgaagcagat cgccacatgc 19381 cttatgctcg taaactttat cagctgccac gcaaagaaaa acgtcagggt ttcttggcgt 19441 taatgcagtc taaaaaagag gaggaaggca atgaccttta aaccattttt aacagatcaa 19501 gaaatagctt ccttacagat tcaagaagct cagtctgata agaaacaaaa acgaacgcct 19561 gagcaaattg aggctattta cacttcagga accaatattt tggtttctgc ctctgctggt 19621 tcaggaaaaa cctttgtgat gattgagcgg attatggata aaattctgcg tggtgtaacg 19681 attgatcagc tttttatttc cacctttacc gttaaggctg ctggtgagct taaggagcgt 19741 ttagaaaaga aaatcaccga gcaattgcgt ttgacaaatg acactgccct caaacaattt 19801 ctcagcgaac aactcttggg attgcagact gcggatattg gaaccatgga tgcctttacc 19861 caaaaattag tgacacagta cggctatacc ttgggaattt caccgaattt tcgaatttta 19921 caagataaaa gcgagcagga tcttcttaaa aatgatgtct ttgacgacct ttttactgat 19981 tacagaactg gcgatcaagc agagcttttc actaagcttg ttaggaattt tgctggaaat 20041 cgaaaagatt cgagcaattt tagacaaatc atttacaaaa tttatgattt cagtcaggca 20101 accgataatc cccaaagatg gcttttggag aatttcttaa aaggagccaa tacctataag 20161 gatttttcag ctattcctga acaagaagtg aaagactttt tgaatacttt gcaggaaact 20221 gctctcgctc ttcgtgatgt gacggattta gaagattaca agcaagtaac ggctaaagga 20281 actccaacag cagcttatca aaggcacctt aaaatgattg agcagctgca ggattgggtc 20341 ttgcattttg acagtctata tgggcgagat ggtctgggga aattagcaaa tgacattgca 20401 acactcattc catctggtaa cgatgtgaca gtagcaggag ttaaatatcc tgtctttcgc 20461 agtttgcaca gtcgcttaag aggactaaaa cacttggaaa caattttcaa gtatcaggat 20521 caaagtctgc ctttgttaca agtactgcaa tcttttaccc ttgatttttc aaaacaatac 20581 ctgcaagcaa agatgcagga aaatgccttt gagttttcag acattgcaca ttttgctatc 20641 cagattttag aagaaaatga tgctattcgt cagctttata tagataagta tcatgaagtc 20701 atggtagatg aatatcagga taataatcac acccaagagc gcatgttgga acttttatct 20761 aatgggcgca atcgcttcat ggtaggagat attaagcaat ctatctatcg gtttcgtcag 20821 gcagatccac agatttttaa tcaaaaattt aaggattttc aagaacatcc agagcatgga 20881 aaactcattc tcttaaaaga aaactttcgg agtcagtcag aagtcttaga tgctaccaat 20941 agtgttttta cgcaccttat ggacgaaacg gttggagaaa ttctctacga tgatacccat 21001 cagcttgtgg ctggcagttc agctcagaaa attccttatc cgcaaaatga aacacaagtc 21061 ctcatctatg atacaaagga tcaacaaaat caagatcttg ctgtagaaga tgatagtgat 21121 caaatcagtc taggtgaggt caaattggtt gctaaggaga ttattcgcct tcataatgaa 21181 gaaaaggttc agtttgaaga tattaccttg cttgtctcat cgcggacacg caatgatggt 21241 atcttgcaaa cctttgatga ttatggcatt cctttggtga ctgatggtgg tgaacaaaac 21301 tatctgaaat cagtggaagt tatggtcatg ttggatactc tgcgttctat tgataatcct 21361 ctcaatgatt atgctctagt agctcttttg cgttcaccta tgtttgcttt tgatgaggat 21421 gacttggctc gtctagctct gcaaaatcta ccagatcaac acaaacaaaa tctttatgaa 21481 aagatggaga atgccagaaa tggccaaggc cagcaagttc agttgatcac agaagcattg 21541 tctgccaaat tggatgcttt ctttgaaact ttcctcagtt ggcgtgagtt tagtctcttg 21601 aattcgctct atgatttgat ttggaaaatt tataatgata agttttacta tgattatgtg 21661 ggaagtttac caaaatctga acagcgccaa gccaatcttt atgctctagc cctgcgtgcc 21721 gataactttg agaaaacagg ttttaagggg ttgtcgcgct tcattcgcat gattgacaaa 21781 attctagaaa atcaaaatga cttggctgat gttgaagtcg ctttacctaa aaatgcagtg 21841 acgctgatga cgattcataa gagtaaggga ttggaattta aatatgtctt tattcttaat 21901 attgataaaa aatttagcat tcaagatatg acatcaccct taatcttatc gcggcaaaac 21961 ggtgttggta tcaagtatat tgctgatatg aaagaagaac tagaggaaaa attattgcca 22021 acagttaaag tcagcatgga tactcttcct tatcaactga ataagcgtga gcttcgtctg 22081 gcaaccctat ctgaacaaat gcgattgctt tatgttgcca tgacacgatc tgaaaagaaa 22141 ctttacttgg tcggaaaagg cagtcaggaa aaattagggg atcagtatga tggcaaatca 22201 gaaaacaatc atttgccagt agctgatcgt gaacactatt tgacttttca ggattggctt 22261 ttggcgattg aagctgctta tgctgccgat gaacttcatt ttaaaacaag ttttataacg 22321 gatgaagact taacagaaga taaaatggga agtttagaag cagagcaggc ttatgatgcg 22381 gataatctta aggataatcg gcaatcagat aatattgcgc gtgctttaga tatgttagaa 22441 gcagttgaaa aattaaatca acattataag gctgctatcc atttaccaac tgttcgtac //