LOCUS       NZ_CP023477            10578 bp    DNA     circular CON 18-JUL-2024
DEFINITION  Streptococcus mutans strain LAR01 chromosome, complete genome.
ACCESSION   NZ_CP023477
VERSION     NZ_CP023477.1
KEYWORDS    RefSeq.
SOURCE      Streptococcus mutans
  ORGANISM  Streptococcus mutans
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2088369)
  AUTHORS   Abranches,J., Lemos,J., Kajfasz,J. and Aviles-Reyes,A.
  TITLE     Whole genome sequence and phenotypic characterization of a Cbm+
            serotype e strain of Streptococcus mutans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2088369)
  AUTHORS   Abranches,J., Lemos,J., Kajfasz,J. and Aviles-Reyes,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-SEP-2017) Oral Biology, University of Florida, 1395
            Center Drive POBOX 100424, Gainesville, FL 32610, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: celera v. 9/01/15
            Genome Coverage                   :: missing
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002995555.1-RS_2024_07_18
            Annotation Date                   :: 07/18/2024 01:10:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,086
            CDSs (total)                      :: 2,002
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 41 of 72
            Pseudo Genes (incomplete)         :: 35 of 72
            Pseudo Genes (internal stop)      :: 29 of 72
            Pseudo Genes (multiple problems)  :: 25 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:47:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 284415
            Orig. end                         :: 294993
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP023477.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Dr. Jacqueline Abranches.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10578
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10578
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10578
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5578](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5578
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    273..291
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 23.5"
                     /tool="antismash"
     gene            702..2012
                     /gene="der"
                     /locus_tag="CO204_RS01505"
                     /old_locus_tag="CO204_01505"
     CDS             702..2012
                     /EC_number="3.6.5.-"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0003924 - GTPase activity [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /GO_function="GO:0043022 - ribosome binding [Evidence IEA]"
                     /GO_process="GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="der"
                     /gene_functions="other (smcogs) SMCOG1227: ribosome
                     biogenesis GTP-binding protein YsxC"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /locus_tag="CO204_RS01505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CO204_01505"
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="WP_002287707.1"
                     /transl_table=11
                     /translation="MALPTVAIVGRPNVGKSALFNRIAGERISIVEDVEGVTRDRIYTK
                     AEWLNRQFSIIDTGGIDDVDAPFMEQIKHQADIAMTEADVIVFVVSAKEGITDADEYVA
                     KILYRAHKPVILAVNKVDNPEMRSAIYDFYALGLGDPYPVSSAHGIGTGDVLDAIVDNL
                     PTEAQEESSDIIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTTFTDEEG
                     QEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREYDKRIAGF
                     AHEAGKGIVVVVNKWDTIKKDNHTMAQWETDIREQFQYIPYAPIVFVSALTKQRLHKLP
                     DMIKQISQSQNTRIPSAVLNEVIMDAVAVNPTPTDKGKRLKIFYATQVSVKPPTFVIFV
                     NEEELMHFSYLRFLENQIRQAFVFEGTPIRLIARKRK"
     gene            2087..2713
                     /locus_tag="CO204_RS01510"
                     /old_locus_tag="CO204_01510"
     CDS             2087..2713
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298155.1"
                     /locus_tag="CO204_RS01510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CO204_01510"
                     /product="hypothetical protein"
                     /protein_id="WP_002265453.1"
                     /transl_table=11
                     /translation="MFENLKAYLTQQGFKYIKPEKAGSLAEEMIALKTLGQSARAEFTE
                     IAKVLALKVSPFEMMRVSNWANQAQVARPHFWCYYKQPEDNQDDVGLAIRLYGNSANFG
                     ISVEVSFIERKKSKATLAKQHKVLDIPIAEPLYYFAQEKSESHRVSGTEAYRQMLRQKV
                     ADGQVRKVLVKYDIPISEGSDLVILTDQLAQGFDKLMPYYQATKS"
     gene            2760..3398
                     /locus_tag="CO204_RS01515"
                     /old_locus_tag="CO204_01515"
     CDS             2760..3398
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1188: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302658.1"
                     /locus_tag="CO204_RS01515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CO204_01515"
                     /product="VTT domain-containing protein"
                     /protein_id="WP_106006673.1"
                     /transl_table=11
                     /translation="MFFIDFILHIDNYIYSIANTVGLWTYLILFLVIFVETGVVILPFL
                     PGDSLLFAAGALAANPKMHFSVLVFGILFFIAAFVGDTCNFFIGRTAGYRLVHHKFFSQ
                     FIKEDNIKDAELYFEKYGSTSIILGRYIPIIRTFVPFVAGLSQFPVSSFIKRSFIAALS
                     WTVIATGAGYLFGNIPFVKTHFSAIILAIVFVTLLPSIITVVKSSRKRK"
     misc_feature    complement(2780..2794)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 22.15"
                     /tool="antismash"
     misc_feature    complement(3837..3850)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.01"
                     /tool="antismash"
     misc_feature    3837..3851
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 22.99"
                     /tool="antismash"
     gene            3889..4083
                     /locus_tag="CO204_RS01520"
                     /old_locus_tag="CO204_01520"
     CDS             3889..4083
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002274099.1"
                     /locus_tag="CO204_RS01520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CO204_01520"
                     /product="hypothetical protein"
                     /protein_id="WP_106006674.1"
                     /transl_table=11
                     /translation="MLAGIEGGVNWDRVFGGTTVYLGATMALCATTGPIGWGVRAAYLA
                     TCAASGAYIMVWQPNLGIG"
     misc_feature    3904..3913
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 17.05"
                     /tool="antismash"
     gene            4111..4470
                     /locus_tag="CO204_RS01525"
                     /old_locus_tag="CO204_01525"
     CDS             4111..4470
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002960574.1"
                     /locus_tag="CO204_RS01525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CO204_01525"
                     /product="hypothetical protein"
                     /protein_id="WP_106006675.1"
                     /transl_table=11
                     /translation="MRRKSSFIICIAIIIISNMLMMTVINNGEEKLSNLFVIAIALVCF
                     PGFWWAIDNEMISLIRIESSALLLLAVLPAMRLINRIDTLPTMVNSTVIIVALGLITVL
                     LVAGIARWIAKKKKD"
     gene            5001..5276
                     /locus_tag="CO204_RS01530"
                     /old_locus_tag="CO204_01530"
     CDS             5001..5276
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002279082.1"
                     /locus_tag="CO204_RS01530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CO204_01530"
                     /product="Blp family class II bacteriocin"
                     /protein_id="WP_002280334.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 1.8e-10,
                     bitscore: 38.0, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTQALENFENMNAQALAAVEGGGVNQCVAGTTGKVIEGALAGGA
                     AGATIAAAVSAPVAEGGGIGPAAGAGIGAVLGGISNGLYGASQYCN"
     gene            5309..5578
                     /locus_tag="CO204_RS01535"
                     /old_locus_tag="CO204_01535"
     CDS             5309..5578
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002278332.1"
                     /locus_tag="CO204_RS01535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CO204_01535"
                     /product="hypothetical protein"
                     /protein_id="WP_002279083.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 1.1e-09,
                     bitscore: 35.4, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTQTLEHFDVLNADLLAKVQGGDWQCTSGVLGGMVYGALTGAGA
                     GAAIGAAVSAPAAEGGGVGPAAGALLGGTYGAIGGILQGATACE"
     gene            complement(6140..7414)
                     /gene="serS"
                     /locus_tag="CO204_RS01540"
                     /old_locus_tag="CO204_01540"
     CDS             complement(6140..7414)
                     /EC_number="6.1.1.11"
                     /GO_function="GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /GO_function="GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="serS"
                     /gene_functions="other (smcogs) SMCOG1162: threonyl-tRNA
                     synthetase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002279084.1"
                     /locus_tag="CO204_RS01540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CO204_01540"
                     /product="serine--tRNA ligase"
                     /protein_id="WP_106006676.1"
                     /transl_table=11
                     /translation="MLDIKRIRNDFDQIAEKLARRGVDEKTLHDLKELDLKRRQLLIKS
                     EEAKAERNTASAAIAQAKRNQEDASEQIADMQKLSAEIKALDAQLVEIDGQLKTFTTTL
                     PNIPADDVPLGADEDENVEVRCWGKPRQFDFDIKAHWDLGEDLDILDWERGSKVTGSRF
                     LFYKGLGARLERAIYNFMLDEHAKEGYTEVIPPYMVNHDSMFGTGQYPKFKEDTFELAD
                     NDYVLIPTAEVPLTNYYRGEIIDGKELPIYFTAMSPSFRSEAGSAGRDTRGLIRLHQFH
                     KVEMVKFAKPEDSYNELEKMTANAENILQKLELPYRVITLCTGDMGFSAAKTYDLEVWI
                     PAQNTYREISSCSNTEDFQARRAQIRYRDEVDGKVKLLHTLNGSGLAVGRTVAAILENY
                     QNADGSVTIPEVLRPYMGGTEMIVS"
     misc_feature    complement(7466..7479)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 21.0"
                     /tool="antismash"
     misc_feature    7596..7610
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 23.18"
                     /tool="antismash"
     misc_feature    complement(7880..7894)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: medium, score: 24.91"
                     /tool="antismash"
     misc_feature    complement(7890..7901)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: strong, score: 19.35"
                     /tool="antismash"
     gene            7937..9010
                     /locus_tag="CO204_RS01550"
                     /old_locus_tag="CO204_01550"
     CDS             7937..9010
                     /EC_number="2.3.1.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019314203.1"
                     /locus_tag="CO204_RS01550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CO204_01550"
                     /product="acyltransferase"
                     /protein_id="WP_002275356.1"
                     /transl_table=11
                     /translation="MNKKNEKIKSLPYNAGIDFLRIFAMFMIVVTHVLGKGGIRSTVKE
                     NFDLYYFVTWGIQVSAYSAVNCYALISGYVGVDSPYRYSKCLSLWLQVFFYTLLFILFF
                     TFLQKPITYHDWIQAFFPIITGQYWYVTAYFGLLIFMPILNLALRKLNTKDLKHIVLLA
                     IIFFSVLPALLNTKVDEFSFAKGFSMNWLSVLYIIGAYLKRLDLKRLFSRKFLLFLCLM
                     AMAVTFITKVFIGDIWYWYTSPTLLCEAVTIFIFFVTLDIKKTGRLFRIIKRMAPATLG
                     VYLFHLNPLLVKFLLKDGFESFVTAPIWLFPFLILGTALLIYLVSTLVELLRIKLFSYL
                     KVRHLILKLDTYLPFDN"
     gene            complement(9249..9617)
                     /locus_tag="CO204_RS01555"
                     /old_locus_tag="CO204_01555"
     CDS             complement(9249..9617)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002264180.1"
                     /locus_tag="CO204_RS01555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CO204_01555"
                     /product="DUF956 family protein"
                     /protein_id="WP_002263350.1"
                     /transl_table=11
                     /translation="MAQSINTKVEYTGKGVSYLGVGGKVGKFLLGDRSFEFYNDANVEH
                     FIQIPWSSIIEIGANVNRKKVSRHFEIITDKGKFLFASSDSGKILKIARQHVGNDKVVR
                     YPSLLQKIASFFKRKKKG"
     misc_feature    complement(9638..9654)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 24.42"
                     /tool="antismash"
ORIGIN
        1 ccaagggcta tcagccgctt cttgtaatga atgaaggtta tgcagatgtc gcctataaag
       61 agacaaaaga actgattgct gcaaagaaga ctcaagcgat ttctgaccgg attaatgtgg
      121 ttagtttacc aagatcttat aagaacatct cctttgatga tattaatctt gatgatgtca
      181 agcgactaga tgtttttaaa agggtagctg attttgtgga gcagtatccg aatcctgagc
      241 aaaaaggact ttatctttat ggtgatatgg gaattggtaa gtcttacctc atggcagcca
      301 tggctcatga attgtcagaa caaagaggag ctgcaacgac actgctgcat tttccgagtt
      361 ttacgattga tgtcaagaat gccatcaaca caggaactgt taaggaagaa attgatgctg
      421 tcaagacggc tgatattctg attcttgacg atattggtgc tgaacaaagc acttcttgga
      481 ttcgtgatga agttctgcag gttattttac aatatcggat gttagaagaa ttgccaacct
      541 tttttacctc taattatagt tttaaggatt tagaggcgaa attagcgaat ataaaaggga
      601 gcgatgaaac ttggcaggct aagcgtgtga tggagcgtat tcgttattta gcaaaagaaa
      661 ttcaccttga aggcgaaaat agaagataag aaggagaaat aatggcttta ccaacagttg
      721 ctattgtcgg acgcccaaat gtcggcaaat cagcgctttt taatcgtata gctggtgagc
      781 gtatttccat cgttgaggat gttgaaggag taacaagaga ccgcatttat acaaaggcgg
      841 aatggcttaa tcgccaattt tcaatcattg atacaggcgg tattgacgat gttgatgccc
      901 cctttatgga acaaattaag catcaagctg atattgctat gacagaggct gatgtgattg
      961 tttttgtggt atctgctaag gaaggtatca cagatgccga tgaatatgta gcaaagattc
     1021 tttatcgggc acacaagccg gttattttag ctgttaataa agttgataat cctgagatgc
     1081 gcagtgctat ttatgatttc tatgccttgg gactggggga tccttatcct gtttcttctg
     1141 ctcatggtat tggtacagga gatgtccttg atgctatcgt tgataattta ccgacagaag
     1201 cgcaagaaga gagttcagac attattaagt ttagtttgat cggacgtccc aatgttggta
     1261 aatccagttt aatcaatgct atcttaggag aagatcgtgt tatagcatca ccagtagctg
     1321 gtacgacccg tgatgcgatt gatacaacct tcacggatga agagggtcaa gaatttacca
     1381 tgattgacac agctgggatg cgtaagtcag gtaaagttta tgaaaataca gagaaatatt
     1441 ctgttatgcg tgctatgcgt gcgattgatc gctcagatat tgttttgatg gtgttgaatg
     1501 ctgaggaagg tattcgtgaa tacgacaagc gcattgctgg ttttgctcat gaggctggta
     1561 aagggattgt tgtcgttgtg aataagtggg atactattaa aaaagacaat catacaatgg
     1621 ctcagtggga gaccgatatt cgtgagcagt ttcaatatat tccctatgca ccgattgttt
     1681 ttgtatcagc tcttacgaaa cagcgtcttc ataagctgcc ggatatgatt aagcaaatca
     1741 gtcaaagtca aaacacccgc attccttcgg ctgttttaaa cgaagtgatt atggatgctg
     1801 ttgcagttaa cccaacaccg acagataagg ggaaacggct gaagattttt tatgccactc
     1861 aagtatcagt caagccgcca acctttgtta tttttgtcaa tgaagaagag ctgatgcact
     1921 tttcctacct gcgcttttta gaaaatcaaa ttcgccaagc ttttgttttt gagggaaccc
     1981 ctatccgctt gattgcaagg aaacggaaat aaggtagtct ttgctttctt acttttaggc
     2041 ttttaacaat aaagcaagca tagataggtt gtatggaaag gaaaaaatgt ttgaaaatct
     2101 aaaagcttat ttgacacagc aaggttttaa gtatattaaa ccagaaaagg ctggttcctt
     2161 ggctgaagaa atgatagctt tgaaaacctt aggacaatct gcgcgtgctg aatttacaga
     2221 gatagctaag gtgcttgctc ttaaagtttc tccttttgag atgatgcgtg tcagtaattg
     2281 ggctaatcag gcacaagttg ccagaccgca tttttggtgc tattataaac aacctgaaga
     2341 taatcaggac gatgttggac ttgctatcag gctttatggg aattcagcta attttggtat
     2401 ttcagtagag gtcagtttta ttgaacgcaa aaaatcaaag gcgactctag ctaaacagca
     2461 taaagtttta gacataccga ttgctgaacc gctttattat tttgctcaag agaaaagtga
     2521 aagtcatcgg gtttcaggaa cagaagctta tcgtcagatg ttacggcaaa aagttgctga
     2581 cggtcaagtt cgaaaagttt tggtcaagta tgacatacct atatcagagg gatcagatct
     2641 agttatacta actgatcaat tggctcaagg ctttgataaa ttgatgccat attatcaagc
     2701 aactaaatca taggtattaa acagcagtct aaagggattt aggattatta gaaaaaatta
     2761 tgttttttat tgattttatt ttacatattg ataactatat ttatagtatt gccaataccg
     2821 ttggcttgtg gacttaccta attctatttt tagtaatctt tgtggaaacg ggtgtcgtca
     2881 ttcttccttt tctgccggga gacagcctct tatttgcagc aggtgcctta gcagccaatc
     2941 ctaagatgca ctttagtgtt ttagtttttg gtattttatt tttcatagct gcttttgttg
     3001 gagatacttg taatttcttt atcggtcgta cggctggtta tcgtttggtg catcacaaat
     3061 ttttcagtca atttattaaa gaagataata tcaaagatgc ggagctttat tttgaaaaat
     3121 acggttcaac ttctatcatt ttaggacgtt atatcccgat tatcagaact tttgttcctt
     3181 ttgtagctgg ccttagtcaa ttccctgtgt cttcttttat taaacgcagt tttatagctg
     3241 ctttatcttg gacagtgatt gctacaggag ctggctatct ttttggtaac attccttttg
     3301 ttaaaacaca tttttctgct attatcttgg ctattgtttt tgtgacttta ctaccaagta
     3361 ttattactgt cgtcaaatcc agcagaaaaa gaaaataatt ataaaatcaa ttgaccaacg
     3421 gctgattaac agaaaaaagc agtaatgctg ttaatcagtc gttttttgcc gtatagaata
     3481 tgtaggaaga gttgaacata atcaaagaaa tcttaatcct gtagttgtcg tttactgata
     3541 tcattacttt actttattta atttataaaa aggctgcatt catctgcacc ttatttttca
     3601 tagtaagctc tattttgatt ctttgtgatt aatcatgaag tctcgttaaa tctgtaggaa
     3661 acaagagaat gttctttctc tgttagtttt agagttgtaa gaaagaaaag gaagttgatc
     3721 tgtcttaaat tttaattgta aacttattag acttctattt tactttaatt taaagaaaaa
     3781 tgctctagat gtttcaaata tattaaacat ttatgctgaa tcttaaattt tcttggtttt
     3841 ttcttaaaat tacttaatat agtaactata aataaaaatt tagatgatat gcttgccggt
     3901 atagaaggcg gagtcaattg ggatcgtgtt tttggtggta ctacagttta tcttggtgcg
     3961 actatggcgc tttgcgcaac taccggtcca attggttggg gagttagagc tgcatacctt
     4021 gctacttgtg cagcttcagg tgcatacatt atggtctggc aacctaactt aggcattggt
     4081 taaattagtc tacgttttga ggtggttatc gtgagaagaa aaagttcttt tatcatttgt
     4141 atagcaatta tcatcatatc aaacatgctt atgatgacag ttattaacaa tggtgaggag
     4201 aaattatcaa atctatttgt aatagcgatt gcattagttt gctttccagg cttttggtgg
     4261 gcaattgata atgagatgat ttcacttatc cgcattgaat cttctgcgct acttcttctc
     4321 gctgtccttc ctgccatgcg tttaatcaat agaattgaca ccttacctac tatggtgaac
     4381 tctacagtaa ttattgttgc tttgggctta ataacagtac tgttagtggc tggaattgcc
     4441 agatggatag ccaaaaagaa gaaggattga tttaataaca gttaagactg ttccaaataa
     4501 gtttcaaact gaagataaag tccacagaaa acatttgaca cattttcaat tgtagcaggt
     4561 ggctaatcta acatttagag aggatcaagt tggttctctc tatttgtatg ttcaaagcga
     4621 ggagtctttt ggtttcgaag tatctgtttt tctaaggctc aaaaagtcac gcttggtgtt
     4681 ttataaatat ttagttattt aagtttgaag ttagaatagc ttttatcgaa actgtcaatt
     4741 atttctggaa atcttcataa agggaataat aatttcggtg aataagcatt gtttctttct
     4801 gagtaaacta atagagtcta tttattttta actaatttat ttgaaagttt aataaacaat
     4861 ctataatttt tcttctcttt aacataaaca ttaattctaa ctttctttag actattgggg
     4921 tttacaagct attaaaaagt ttctataatg tgcttgtcag atgagaaagc tgacaaataa
     4981 aaagaaaagg ggtttatctt atgacaactc aagcattgga aaattttgaa aacatgaacg
     5041 ctcaagcact cgctgctgtt gaaggtggtg gtgttaatca atgcgttgca ggcacaactg
     5101 gtaaggttat agaaggagcc ctcgcaggtg gcgcagcagg tgcaactata gctgctgctg
     5161 tctctgcacc tgttgcagaa ggtggtggaa tcggtcctgc agcaggtgcg ggtattggtg
     5221 ctgttttagg tggtattagc aatggattat atggtgcgtc acaatattgt aattaattta
     5281 ttcactacaa aaaaagagga ggtatcttat gacaactcaa acattagaac actttgatgt
     5341 attaaatgca gatttgcttg ctaaggtaca aggtggcgat tggcaatgta cgtcaggagt
     5401 tttgggtggt atggtgtatg gcgctcttac aggggcagga gcaggagcag ctataggtgc
     5461 tgctgtttct gctcctgctg cagaaggtgg cggagtcggt cctgcagcag gggctttact
     5521 tggtggaacc tatggtgcaa ttggtggtat ccttcaaggt gcaactgctt gtgaataaaa
     5581 ctggcagatt tggttgattc tcaactgcag tactcgttaa acctttattt ttgaatacaa
     5641 gaagaagttg cttgtacaat taaggtaagg acatctacta tttacactaa atggtagatg
     5701 tccttttttg tgtgttaaat tatctttaag gagatgaccg tatattacat ctaataacct
     5761 aacgtctttc acatagtatt caggttatta ttactcatag aagtgatttc cttattcaaa
     5821 gaattattgg gcgccaatct ttctatttct aatgttgctt ttgtcaaaag gcattatcag
     5881 ataaaaactg aggcctttta tgccccttgt aaagagttca tctcttatat ttaatttcta
     5941 cttacctata agtagccaca aatgggagtg atgtatacca caaaatcctc ataataagta
     6001 gatagcaatc ctataatttg attattgttt agcacgctaa aaaataaatc atctgaatga
     6061 actaatgttg ctgatgttca ttcagatgat gttttatata tagtattaag gattaactta
     6121 ataagattgt tgcaatcagt taagaaacaa tcatctcagt gccacccata taaggacgaa
     6181 ggacttcagg aatcgtcacg gaaccatctg cattttggta attttcaaga atggcagcaa
     6241 cggtacgacc aacagctagt cctgaaccgt ttagggtgtg aagaagtttc accttgccat
     6301 cgacttcatc acggtaacga atttgcgcac gacgtgcttg gaaatcttct gtattggaac
     6361 aagatgaaat ttcacgataa gtattttgtg cgggaatcca gacttctaag tcataggtct
     6421 tggcagccga aaagcccatg tcgccagtac acaaggtaat aacccgatat ggcaattcta
     6481 atttttgcag gatattttca gcattggctg tcattttttc caattcatta taagaatctt
     6541 ctggtttggc aaatttgacc atttctactt tatgaaattg atggaggcgg ataagtccac
     6601 gcgtatcacg accagcagaa ccagcctccg aacgaaaaga agggctcatg gctgtaaagt
     6661 aaataggaag ctctttacca tcgatgattt caccgcggta gtagttggtt aaaggcactt
     6721 cagcagttgg aatcaagaca taatcattgt cagctaattc aaaggtatct tctttaaatt
     6781 ttggatactg tcctgtgcca aacatagaat catgattgac catataaggc ggaataactt
     6841 ctgtatagcc ttctttagcg tgttcatcta gcatgaaatt atagatagca cgttctaggc
     6901 gggctcccaa acctttgtaa aagaggaaac gcgaacctgt tactttagaa ccgcgttccc
     6961 aatcaagaat atcaagatct tcgccaagat cccagtgcgc tttgatgtca aaatcaaatt
     7021 gacgtggttt tccccagcag cgcacttcaa cattttcatc ttcgtcagca ccaagaggaa
     7081 catcatctgc tggaatattt ggaagagttg ttgtaaatgt ctttagttgc ccatcaattt
     7141 cgactaattg ggcatcaagt gctttgattt cggcagataa tttttgcata tcagcaatct
     7201 gctctgatgc atcttcttga ttacgcttag cttgagcgat agctgcagaa gcagtattgc
     7261 gttcagcttt agcttcttct gatttgataa gcagttggcg acgtttaaga tccagttctt
     7321 tcaaatcgtg tagtgtcttt tcatcaaccc cacgtcttgc caatttttca gcaatttgat
     7381 caaaatcatt acgaatgcgt ttaatatcta acataaataa gataccaagc ccaaaaaagc
     7441 aaagcaaaaa taggaatttt gacaatgaac gtttgttttc taggaaaact attttttgca
     7501 cagcttatag ggcaggttca gttaactttt ttacctaacc aaaccttcct ttctatattt
     7561 tataaaaaac acagacatgt tcttgctgga gcgcttgggc gcggttccat ccagcttcac
     7621 aatgactgtg ctcttaatta cgataatcat ttaataaaga ttgtctcatt cagtcttgga
     7681 aactggatgg aatcatctat aacggtagaa tttcggtcaa tcactctatc tgctcgcagc
     7741 tgccgcagac tttctgcaaa agaaaaggac ctacttatcc gttgttctta ttatacagga
     7801 tttaaaaaag aatgtaaata aagattgttt aaaaaatgat attggaaaat ttcagtttat
     7861 gaaagacaat taacaaagtt tctcaaaatg aaaacatttt cccactcact tttttcctat
     7921 gttaaaataa agcattatga ataagaagaa tgaaaaaata aaaagtttac catataatgc
     7981 tggaatagat ttcttacgta tttttgccat gtttatgatt gtggtgacgc atgttttggg
     8041 gaaaggcggc attcgctcaa ctgtaaaaga gaattttgac ctttattatt ttgtgacatg
     8101 gggaattcaa gtatctgcct atagtgcggt taattgctat gctttgatta gtggttatgt
     8161 tggtgttgat tcaccttatc gttattcaaa atgtctctca ttgtggttac aggtcttctt
     8221 ctatacttta ttatttatct tattttttac ttttttgcaa aagcctataa cataccatga
     8281 ttggatacag gctttcttcc ctattatcac aggtcaatat tggtatgtaa cagcttattt
     8341 tggactcttg atttttatgc ctattttgaa tctagcttta agaaagttga ataccaaaga
     8401 tttgaaacat attgtccttt tagcgatcat cttcttttct gttcttcctg cattattgaa
     8461 taccaaagta gatgaatttt catttgctaa gggttttagt atgaactggt tgagtgtact
     8521 gtacattatt ggagcttatt tgaagcggct tgacttaaag cgtttattca gtcgtaaatt
     8581 tttacttttt ctgtgtttaa tggcaatggc agtgactttt ataaccaaag tatttattgg
     8641 agatatctgg tactggtata cctctcccac tcttctttgt gaggcagtga ctatttttat
     8701 cttttttgtc actttagata ttaaaaagac tggtagattg tttagaatca ttaaacgaat
     8761 ggcgccggca acattaggag tttatctttt tcatctaaat ccccttttgg ttaaatttct
     8821 tttaaaagat ggttttgagt catttgtgac tgctcctata tggctttttc cttttttgat
     8881 tttagggacg gctctcctta tttacctcgt ttcgactcta gttgagcttt tacggataaa
     8941 acttttttct tatcttaagg tcagacacct tattcttaaa ttggatactt atctgccgtt
     9001 tgacaattaa aaggcctaat gacctttttt atcttgataa cagtttgatt ttgatgaagt
     9061 tgtctagaaa ctgctgaata tgatttcttt ccagcatatc ctatgagaat ttatagagaa
     9121 aattgactag atcttttcga tttgcaatta aaataacaaa aaaaaagctg aagaaaaaag
     9181 gttagaatat tgaaacagga tgtgctgtta gatgattggt ctaacttaac attctacatt
     9241 ttcttactct aacccttctt ttttcgttta aagaagctag caatcttttg aagtaaactg
     9301 gggtagcgaa ccactttatc attaccaaca tgctgtcttg caatttttag aattttacca
     9361 gaatcagatg aggcaaagag aaattttcct ttgtccgtaa taatttcaaa gtgtctgctg
     9421 acctttttcc gattgacatt ggctcctatt tcaataatac tagaccaagg aatttggata
     9481 aagtgttcca cattagcatc attatagaat tcaaagctac gatcgccaag aagaaatttt
     9541 cccactttgc cacctacgcc taaatatgat acacctttgc ctgtgtattc aacttttgta
     9601 ttaattgact gtgccataaa tttactcctg aataaatata ttttattata tcaaaaaacg
     9661 gaggactgcg ctatttgtta tttggcgttt cctaattagc ttgtatcgct aaattacggc
     9721 tagtccaagg agaagctttt gaggtgggag atggaagcag tgaaactagc ctcatcaaca
     9781 cagcgcccta tcatgagttt ttcaaagcat attactattt tattgaactt atctagcagt
     9841 gccttgtttt tacagttgac aattttttta gtttaaagaa taattaaata gtctaagaaa
     9901 aatcctctaa agtttattaa ctttagagga ttttatctag ttaggaatgt gatgcttaca
     9961 tcaagccgat aacgtgagcg acaataccaa cgataaagag accgatgata atagtgattg
    10021 gagaaacttt tttcttaagc agccacatac ataagaaagt taaaagtaat cccatgagac
    10081 ctggaataag cgcatcgagc tgactttgaa gactgtcgac cttgactgga ccaaggttaa
    10141 gaccgttttt aaagccttca atagcttctt ttaatttatc tccgctaacg cttcctttag
    10201 gaagtgcaac gaaggtttga ccattatcat ttgttagttt tgttgctggt aatgcgagag
    10261 taaatttaat aactacccag cgttctacca aggctgctaa aataaacatc ccaaggatag
    10321 aagctccttt ggtaatatct ttgaggatac cacctgacaa gtctttagta atttcagatc
    10381 ctgccttata accaaactct tgtgtgtacc acaagaaggc catacgaatg atattccagc
    10441 caaggaagaa aatgatagga cccataatat tgcctgcttg agcaagagaa gctccaagag
    10501 cacctaaaat tggacgtact gtgaaccaga aaacagggtc accaacacct gccaaaggtc
    10561 ccatcatacc gactttaa
//
