LOCUS       NZ_PVZV01000004        10396 bp    DNA     linear   CON 13-DEC-2024
DEFINITION  Actinocorallia populi strain A251 contig4, whole genome shotgun
            sequence.
ACCESSION   NZ_PVZV01000004
VERSION     NZ_PVZV01000004.1
KEYWORDS    WGS; RefSeq.
SOURCE      Actinocorallia populi
  ORGANISM  Actinocorallia populi
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Streptosporangiales; Thermomonosporaceae; Actinocorallia.
REFERENCE   1  (bases 1 to 749123)
  AUTHORS   Li,X.
  TITLE     Actinocorallia populi sp. nov., an endophytic actinomycete isolated
            from a root of Populus adenopoda (Maxim.)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 749123)
  AUTHORS   Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2018) College of Light Industry, Textile and Food
            Engineering, Sichuan University, No. 24, First South Section First
            Ring Road, Chengdu, Sichuan 610065, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04 OR MAR-2018
            Expected Final Version            :: yes
            Genome Coverage                   :: 221.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002995495.1-RS_2024_12_13
            Annotation Date                   :: 12/13/2024 14:18:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,861
            CDSs (total)                      :: 7,799
            Genes (coding)                    :: 7,716
            CDSs (with protein)               :: 7,716
            Genes (RNA)                       :: 62
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 18 of 83
            Pseudo Genes (incomplete)         :: 70 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:05:04
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 159650
            Orig. end                         :: 170046
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            PVZV01000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10396
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="2"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10396
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10396
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5396](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5396
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(275..1360)
                     /gene="dapE"
                     /locus_tag="C2865_RS17420"
     CDS             complement(275..1360)
                     /EC_number="3.5.1.18"
                     /GO_function="GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA]"
                     /GO_process="GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /gene="dapE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1247: acetylornithine deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017627511.1"
                     /locus_tag="C2865_RS17420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="WP_106399548.1"
                     /transl_table=11
                     /translation="MDLSMDVAELTRTLVDIESVSGNERAVVAEIEAGLRALPWLEVVR
                     DGDTLVARTHLGRAERVVLAGHTDTVPVNGNLPSRLEGDTLWGLGSCDMKGGLAVLLHL
                     AAVLTEPTRDVTYVAYECEEVSADRNGLKRLAETRPELLAADFAVLMEPTDGEVEGGCQ
                     GTITVDVTARGARAHTARSWMGANAIHAVKPLIDLLDAYEARRPVVDGITYHEGLNAVF
                     ARGGIARNVVPDECVISVNHRFAPDRDLAAAEALLRELFEPLGYEVEVVDRSESARPGL
                     LHPAAQDFVRAVTALEPAEAIEAGRVRAKLGWTDVSLFSALDVPAVNYGPGDPTLAHHR
                     DERVSLATVRDCERRLRAWLT"
     gene            1423..2232
                     /locus_tag="C2865_RS17425"
     CDS             1423..2232
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_process="GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lysine_decarbox"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851540.1"
                     /locus_tag="C2865_RS17425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="WP_106399549.1"
                     /sec_met_domain="Lysine_decarbox (E-value: 1.1e-33,
                     bitscore: 111.7, seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDDDRTDRAHRKKRRQGPAVLRGRALPESTHDQRLLDTRGSVDWL
                     HQDPWRVLRIQSEFVEGFGLLAELGPAVSVFGSARLRPGSRYYELAERVGRGLVEAGYA
                     VITGGGPGIMEAGNKGANEEGLSVGLGIELPFEQELNQHLDIGISFRYFFVRKTMFIKY
                     SQAFVVLPGGFGTMDELFEALTLVQTGKITRFPIVLVGREFWSPLLEWFRGTLTAEGVI
                     SPDDPDLLYLTDDPDEVVHMIVEAHREGNRRREERAAVEAAEEEAGT"
     gene            2317..2757
                     /locus_tag="C2865_RS17430"
     CDS             2317..2757
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="C2865_RS17430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_157963774.1"
                     /transl_table=11
                     /translation="MRTWRTRFAGALAVLGLVAGCSAENTALRQISPGATVYIDGWDDS
                     ADSGDQALVEQKPSGMWVLPVDAPFRVRSGGDFTVKVTLDSRRGFVVTDLGGTLAGKDH
                     DDCLEAGETYAFVDVPATGIRWEGEPRRTDSTNDDCQVIPAE"
     misc_feature    2883..2898
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 17.13"
                     /tool="antismash"
     misc_feature    complement(2893..2907)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.45"
                     /tool="antismash"
     gene            3192..3680
                     /gene="ectA"
                     /locus_tag="C2865_RS17435"
     CDS             3192..3680
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017558125.1"
                     /locus_tag="C2865_RS17435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_232835669.1"
                     /transl_table=11
                     /translation="MSETIVLDHPHPGDGSALWRLAVESRALDVNSPYSYLLWCRDHGR
                     TSVVARDGDGGACGFVTGYLRPAAPDTLFVWQVAVADSHRGRGLAGRMLGWLAEGKPQA
                     RFLEATVTPDNTASTRLFEAFARERGAGLRRSELFAAEDFPVPGHDPEVLFRIGPFPS"
     gene            3733..4983
                     /gene="ectB"
                     /locus_tag="C2865_RS17440"
     CDS             3733..4983
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (21-356). E-value: 7.1e-73.
                     Score: 237.5. Matches aSDomain:
                     nrpspksdomains_C2865_RS17440_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019610153.1"
                     /locus_tag="C2865_RS17440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_106399552.1"
                     /sec_met_domain="Aminotran_3 (E-value: 3.9e-71, bitscore:
                     235.3, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNVFEEVESEVRSYCRGWPTVFNKAQGSRVYDESGRDYLDFFAGA
                     GTLNYGHNHPQLKLKLLGYLVNDEIVHALDAHTVAKRDFLETFQKTVLIPRGLDYKVQF
                     TGPTGTNAVEAALKLARKYTGRETVVSFTNAFHGMSLGSLSVTGNSMKRGGAGVPLNHV
                     FTMPYDNYLDGCTPDFLLFERMLTDNGSGLDKPAAVIVETLQGEGGLNEAGVGWLRGLA
                     DLCKRHEMLLIVDDIQMGCGRTGPFFSFEEAGIVPDIVCLSKSLSGYGLPMAVTLLRPE
                     LDVWEPGEHNGTFRGFNPAFVTATASLDFWRGEGLREQTLAKGEQVAGTLDELADEHDK
                     DIAARRGRGLAQGLVFTDPPVAGRVCAEAFQRGLLMETSGPDGEVAKLLPPLTITGSDL
                     TEGLAIVRESVAAALDA"
     aSDomain        3796..4800
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_C2865_RS17440_Aminotran_3.1"
                     /evalue="7.10E-73"
                     /label="C2865_RS17440_Aminotran_3.1"
                     /locus_tag="C2865_RS17440"
                     /protein_end="356"
                     /protein_start="21"
                     /score="237.5"
                     /tool="antismash"
                     /translation="FNKAQGSRVYDESGRDYLDFFAGAGTLNYGHNHPQLKLKLLGYLV
                     NDEIVHALDAHTVAKRDFLETFQKTVLIPRGLDYKVQFTGPTGTNAVEAALKLARKYTG
                     RETVVSFTNAFHGMSLGSLSVTGNSMKRGGAGVPLNHVFTMPYDNYLDGCTPDFLLFER
                     MLTDNGSGLDKPAAVIVETLQGEGGLNEAGVGWLRGLADLCKRHEMLLIVDDIQMGCGR
                     TGPFFSFEEAGIVPDIVCLSKSLSGYGLPMAVTLLRPELDVWEPGEHNGTFRGFNPAFV
                     TATASLDFWRGEGLREQTLAKGEQVAGTLDELADEHDKDIAARRGRGLAQGLVF"
     gene            5001..5396
                     /locus_tag="C2865_RS17445"
     CDS             5001..5396
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657163.1"
                     /locus_tag="C2865_RS17445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine synthase"
                     /protein_id="WP_106399553.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.1e-51, bitscore:
                     169.5, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVKTLQDVIGTDRDVQAATWCSRRLVLAGEKVGFSLHDTILYAG
                     TSTSMWYANHIEAVYCIGGTGELVNEETGERHDIAPGTMYLLDGHERHTLHAHTELRTI
                     CVFNPPCTGREVHDEHGVYPLIEEGDA"
     gene            5393..6277
                     /gene="thpD"
                     /locus_tag="C2865_RS17450"
     CDS             5393..6277
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017571802.1"
                     /locus_tag="C2865_RS17450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine hydroxylase"
                     /protein_id="WP_106399554.1"
                     /sec_met_domain="PhyH (E-value: 3.5e-36, bitscore: 120.9,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSGHDPYPTRRADRPELIARVDPVVHGGGEGPLSAGELGGFDRDG
                     FLATDRLITPEEVVRYRAELDRLAGDPALRGDERLITEKGSGEVRSVFEVHRISPVFAA
                     LAADPRIVGRARQILGSDVYVHQSRVNYKPGFRGKDFYWHSDFETWHAEDGMPRMRAVS
                     VSISLTPNNPYNGALMIMPGSHRTFVSCVGETPAEHYRESLKDQEVGTPDPESLTKLAD
                     THGIELFTGDAGSAVMFDCNCMHGSNGNISPYPRSNVFLVFNSVENACAEPYAAPGRRP
                     SFLASRDFTPVGG"
     gene            6350..6922
                     /locus_tag="C2865_RS17455"
     CDS             6350..6922
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_process="GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lysine_decarbox"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545029.1"
                     /locus_tag="C2865_RS17455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="WP_106399555.1"
                     /sec_met_domain="Lysine_decarbox (E-value: 9e-36, bitscore:
                     118.4, seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITVFCASSDRIAAKHVEVAAELGAELARRGHTLVTGGGTRSCM
                     GAVARAARAGGGRTVGVVPAGMPGSQEGADTENDELVRTPDMRSRKAEMDRRADGFLIL
                     PGGIGTLEELLEVWTARSLNFHTKPIVVLDPYGVYEPLRTLMDHLLEEGFNRPAAAEAV
                     SYTTEVGQALDLLESEYRRVNEELLEF"
     misc_feature    6957..6975
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 21.25"
                     /tool="antismash"
     gene            7053..8369
                     /locus_tag="C2865_RS17460"
     CDS             7053..8369
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020547083.1"
                     /locus_tag="C2865_RS17460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alkaline phosphatase PhoX"
                     /protein_id="WP_106399556.1"
                     /transl_table=11
                     /translation="MSVSRRGFVAGGAVSIALAGSLDSVFVTAAGAAEGEAYGYGPLVP
                     DPAGVLDLPRGFSYEVLSTEGEALPGGGVVPGAHDGMAAFPGRRHDDLRLVRNHEQEED
                     GTKAVARPELTYDPEAFGGTTTIHVDREARVLDQYVSLAGTADNCAGGVTPWGTWLTCE
                     ETEGFDAQTKSHGWVFEVDPTGRRTVAEPIEPLGRFEHEAVCIDPRDNVVYLTEDAEEP
                     FGLFYRFLPRSHRGGYHCYQAGGALQAMRVPGVSDLAQVMEAGTVLRGVQWLDVPDPSA
                     AKTSTRAQFDDVTRSSKLEGAWWSRHKAYFVASYALREEGAAGDHAGQVWAYDPRRNTL
                     ELELVFKPGGRFESPDNITVSPYGGGVILAEDGDGEQFLVGTDRRGEPFALARNALNDS
                     EFAGVTFSPDGRTLFANRQEGPGTTFAIRGPWQRIGGRR"
     gene            complement(8374..8682)
                     /locus_tag="C2865_RS17465"
     CDS             complement(8374..8682)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="C2865_RS17465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_106400027.1"
                     /transl_table=11
                     /translation="MNARNRRDLVWTGPPGDPEDLLDAGADPAAGELEALVAGGRPVKV
                     TVTGTGSGALAAAAVYAWLGAAAFEYEGDPDELRQVLDMVSAMAGARPPAVGRRALA"
     misc_feature    complement(8675..8686)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 19.28"
                     /tool="antismash"
     gene            8728..9111
                     /locus_tag="C2865_RS17470"
     CDS             8728..9111
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="C2865_RS17470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_157963775.1"
                     /transl_table=11
                     /translation="MPVVLLLAAVVVVAAAVVLASGRGGELREPVEELPPTGLPEDRLP
                     TGTDAAMVKLPKGVWGYHPLLTDEVLGRLVHALMRRESEVAALERRIAELTAYGPGESL
                     GASWLKKPDEPDFLDGPFGRDRA"
     gene            9290..9457
                     /locus_tag="C2865_RS17475"
     CDS             9290..9457
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966491.1"
                     /locus_tag="C2865_RS17475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF3117 domain-containing protein"
                     /protein_id="WP_106399558.1"
                     /transl_table=11
                     /translation="MAAMKPRTGDGPLEVTKEGRGIVMRVPLEGGGRLVVELTPDEARE
                     LGEAIGGVVG"
ORIGIN
        1 accagcgcgc gccgggggag cttctcgcgg tgcgccacgc ccgcgatcgt cgcggcgttc
       61 gccgcctgct ccacctccag ctggatgtcc ccggccgcgg ccgtcgccgt gcagccctca
      121 cccatcagcg ccttgtcgac ctcggagaac aggcggtacc cgccgaaccc gagggcaccc
      181 gccagcacca ccaccgtgac gacgatggcc aagccccgcc cacccatgac cagaccgtac
      241 ctttttccca taaccgttca ggacaaccag ggtctcaggt cagccaggcg cgcaggcgcc
      301 gttcgcagtc gcgcaccgtg gccagggaga cccgctcgtc cctgtggtgg gccagcgtcg
      361 ggtccccggg gccgtagttg accgcgggga cgtccagcgc cgagaacagc gacacgtccg
      421 tccagccgag cttggcccgc acccgccccg cctcgatcgc ctcggcgggc tccagggcgg
      481 tgacggcgcg gacgaagtcc tgcgcggccg ggtgcagcag gccggggcgg gccgactcgc
      541 tgcggtcgac gacctcgacc tcgtagccga ggggctcgaa cagctcccgc agcagggcct
      601 cggcggccgc caggtcgcgg tcgggcgcga agcggtggtt caccgagatc acgcactcgt
      661 cgggcacgac gttgcgggcg atgccgccgc gcgcgaagac ggcgttcagc ccctcgtggt
      721 aggtgatgcc gtcgaccacg gggcggcgcg cctcgtaggc gtccagcagg tcgatcagcg
      781 gcttgacggc gtggatggcg ttggcgccca tccaggagcg cgcggtgtgc gcccgcgccc
      841 cccgcgccgt gacgtccacc gtgatggtgc cctggcagcc gccctcgacc tcgccgtcgg
      901 tgggctccat gagcaccgcg aagtcggcgg ccagcagctc cggacgggtc tcggccagcc
      961 gcttgagccc gttgcggtcg gcggagacct cctcgcactc gtacgccacg tacgtcacgt
     1021 cgcgggtggg ctcggtcagg accgcggcca ggtgcagcag gacggcgagc ccgcccttca
     1081 tgtcgcagga gcccagcccc cacagggtgt cgccctccag ccgggacggc aggttgccgt
     1141 tcaccggaac ggtgtcggtg tggccggcca gcacgacgcg ctcggcgcgg cccaggtgcg
     1201 tgcgcgcgac cagggtgtcg ccgtcccgca cgacctccag ccagggcagc gcccgcagcc
     1261 cggcctcgat ctccgcgacg accgcccgct cgttgccgct caccgattcg atgtccacca
     1321 gggtgcgcgt cagctcggcc acgtccatcg agagatccat gttcagcgag cttactttcc
     1381 gcgtcgggtc cgttggggct ttcgggcggt taacgtgacc ggatggatga cgatcgcacg
     1441 gacagggcac acaggaagaa gcgccggcag ggcccggcgg tgctgcgcgg cagggcgctg
     1501 ccggagagca cccacgacca gcggctgctg gacacccggg gttcggtgga ctggctgcac
     1561 caggacccgt ggcgggtgct gcgcatccag tcggagttcg tggagggctt cggcctgctg
     1621 gccgagctgg gcccggcggt gagcgtgttc ggctcggcgc ggctcaggcc cggctcgcgg
     1681 tactacgagc tcgccgagcg ggtggggcgc ggcctggtgg aggccggcta cgcggtgatc
     1741 acgggcggcg gccccgggat catggaggcg ggcaacaagg gcgcgaacga ggaaggcctg
     1801 tcggtcggcc tgggcatcga gctgcccttc gagcaggagc tgaaccagca cctggacatc
     1861 ggcatctcgt tccgctactt cttcgtgcgc aagacgatgt tcatcaagta cagccaggcg
     1921 ttcgtggtgc tgcccggcgg cttcggcacc atggacgagc tgttcgaggc gctcaccctg
     1981 gtgcagaccg gcaagatcac acggttcccg atcgtcctgg tcggccggga gttctggtcc
     2041 ccgctgctgg agtggttccg cggcacgctg accgccgagg gcgtgatctc cccggacgac
     2101 cccgacctgc tgtacctcac cgacgacccc gacgaggtgg tgcacatgat cgtcgaggcg
     2161 caccgcgagg ggaaccgccg ccgcgaggag cgggccgccg tcgaggccgc cgaggaggag
     2221 gcgggtacct gagggaacgg tggggtgaat tgtcgctgaa agttcactta tgtccactat
     2281 ctaaagtggt ggctgttctg gcaccgtcct cggtccatga ggacatggag aacacggttc
     2341 gccggggcgc tggccgtcct cgggctggtg gcgggatgct ccgccgagaa caccgcgctc
     2401 cggcagatct cgccgggcgc caccgtctac atcgacgggt gggacgacag cgcggacagc
     2461 ggtgaccagg cgctggtcga gcagaagccg tcggggatgt gggtgctccc ggtggacgcc
     2521 cccttccgcg tcaggtccgg cggggacttc accgtcaagg tgacgctcga ctcccggcgc
     2581 ggcttcgtcg tcaccgacct gggcggcacc ctggcgggca aggaccacga cgactgcctg
     2641 gaggcggggg agacctatgc cttcgtcgac gtccccgcca cgggcatccg gtgggagggg
     2701 gagccccggc gcaccgactc caccaacgac gactgccagg tcatccccgc ggagtgaata
     2761 aaggggggct cgggcggaat tccggtcccc cttcgccgcg caacatcact cacgaggcgt
     2821 caccgagccg ttccggtccg tcccagacgc ttgagttagg tcacgggttc acatcactca
     2881 tgggtgatga gcgtcacaaa attagacgaa gccgatcagg caacctttcc ctctcttcaa
     2941 gagtcgtccc cgcgggtaga agcgggcgac tccgcgcggg ctcctctcga accgcctgtt
     3001 gaagcgggag aacaagcacg tttcgggcat tcgctctttc atgccgaacg tggcggctgc
     3061 cgagcctgcc agccctaccc ggtatcccgg ggcccaaacc aagaccgccc ttcccggcca
     3121 ccaggggggt cccaccggcg tggctatggt cttttcgcat gaccaaggag cttccatccc
     3181 gagtcagcgg cttgtccgaa accatcgtcc tcgaccaccc gcatcccggg gacggcagcg
     3241 ccctgtggcg gctggccgtc gagtcccggg ccctggacgt caactcccct tacagctacc
     3301 tcctgtggtg ccgggaccac ggcaggacct ccgtggtggc ccgggacggc gacggcgggg
     3361 cctgcggctt cgtcaccggc tacctgcgtc ccgccgcccc cgacaccctg ttcgtctggc
     3421 aggtcgccgt ggccgactcc caccgcggcc gcggcctggc cgggcggatg ctcggctggc
     3481 tcgccgaggg caagccgcag gctcgcttcc tggaggcgac ggtgaccccc gacaacaccg
     3541 cctcgacccg cctgttcgag gcgttcgccc gggaacgggg agccggtctg cgcagaagcg
     3601 agctcttcgc ggccgaggac ttcccggtgc ccggccacga tcccgaagtg ctcttccgta
     3661 tcggcccgtt cccctcgtga gcagcggcct ttccaccccc acccctgaca gcgccaggaa
     3721 aggcggcacc acatgaacgt cttcgaagag gtcgagtccg aggtccgcag ctactgccgc
     3781 ggctggccca ccgtcttcaa caaggcccag ggcagccggg tctacgacga gtccggccgc
     3841 gactacctgg acttcttcgc cggagccggg accctcaact acggccacaa ccacccccag
     3901 ctcaaactga aactgctcgg gtacctcgtc aacgacgaga tcgtgcacgc cctggacgcg
     3961 cacaccgtgg ccaagcgcga cttcctggag acgttccaga agaccgtcct catcccccgg
     4021 ggcctcgact acaaggtgca gttcaccggc cccaccggca ccaacgccgt cgaggccgcg
     4081 ctgaagctcg cgcgcaagta caccggccgg gagacggtcg tgagcttcac caacgccttc
     4141 cacggcatgt cgctgggctc gctgtcggtc accggcaact cgatgaagcg cggcggagcg
     4201 ggcgtgcccc tcaaccacgt cttcaccatg ccctacgaca actacctcga cgggtgcact
     4261 cccgacttcc tgctgttcga gcgcatgctc accgacaacg ggagcggcct ggacaagccc
     4321 gccgcggtga tcgtggagac gctccaggga gagggcgggc tcaacgaggc cggcgtcgga
     4381 tggctgcgcg gcctggccga cctgtgcaag cgccacgaga tgctgctgat cgtcgacgac
     4441 atccagatgg gctgcgggcg gacggggccg ttcttcagct tcgaggaggc cgggatcgtc
     4501 ccggacatcg tctgcctgtc caagtcgctg agcggctacg gcctgccgat ggcggtgacg
     4561 ctgctgcgcc cggaactcga cgtctgggag cccggcgagc acaacggaac gttccgcggg
     4621 ttcaaccccg cgttcgtgac ggcgaccgcc tccctggact tctggcgggg cgagggcctg
     4681 cgcgagcaga cgctcgccaa gggcgagcag gtggccggaa ccctggacga gctcgccgac
     4741 gagcacgaca aggacatcgc ggcccggcgc gggcgcggcc tggcccaggg cctggtcttc
     4801 accgatccgc cggtggccgg gcgggtctgc gccgaggcct tccagcgcgg cctgctgatg
     4861 gagacctcgg gaccggacgg cgaggtggcc aagctgctgc ccccgctcac catcaccgga
     4921 tcggacctca ccgaggggct ggccatcgtg cgcgagtccg tcgccgccgc cctggacgcc
     4981 tgagagaacg gagcacgacc ttgatcgtca agaccctgca ggacgtcatc ggcaccgacc
     5041 gcgacgtgca ggcggccacg tggtgcagca ggaggctggt gctcgccggg gagaaggtcg
     5101 gcttctccct gcacgacacc atcctgtacg cgggcaccag cacctcgatg tggtacgcca
     5161 accacatcga ggcggtctac tgcatcggcg gcaccggcga gctggtgaac gaggagacgg
     5221 gcgaacgcca cgacatcgcc cccggcacca tgtacctgct ggacgggcac gagcggcaca
     5281 ccctgcacgc ccacaccgag ctccgcacga tctgcgtgtt caacccgccg tgcaccggcc
     5341 gcgaggtcca cgacgagcac ggcgtctacc cgctgatcga ggagggggac gcgtgagcgg
     5401 ccacgacccc taccccacgc gccgcgccga ccgccccgag ctgatcgcgc gggtggaccc
     5461 ggtggtccac ggcggcggcg aggggccgct gtccgccggg gagctcggcg gcttcgaccg
     5521 cgacggcttc ctggcgacgg accggctgat caccccggag gaggtcgtgc gctaccgggc
     5581 cgagctggac cggctcgccg gcgaccccgc gctgcgcggc gacgagcggc tcatcaccga
     5641 gaaggggtcc ggagaggtcc gctcggtctt cgaggtgcac cggatcagcc cggtcttcgc
     5701 cgcgctcgcc gccgacccgc gcatcgtcgg acgggcccgg cagatcctcg gctcggacgt
     5761 ctacgtgcac cagagccggg tcaactacaa gcccggcttc cgcggcaagg acttctactg
     5821 gcactccgac ttcgagacct ggcacgccga ggacggcatg ccgcggatgc gcgcggtgag
     5881 cgtctcgatc tcgctcaccc ccaacaaccc gtacaacggc gcgctgatga tcatgccggg
     5941 ctcgcaccgg acgttcgtct cctgcgtggg ggagacgccc gccgagcact accgggagtc
     6001 cctgaaggac caggaggtcg gcacccccga cccggagagc ctgacgaaac tggccgacac
     6061 ccacgggatc gagctgttca ccggcgacgc gggctcggcg gtcatgttcg actgcaactg
     6121 catgcacggc tcgaacggca acatctcccc ctacccgcgc tcgaacgtct tcctggtgtt
     6181 caacagcgtg gagaacgcct gcgcggagcc gtacgccgcg ccgggccgca ggccgtcctt
     6241 cctggcgtcc cgcgacttca ccccggtagg gggctgacct ccgccggagc ccctccggcc
     6301 ctatgatcgc cggaaaagcc gagggattac ggaggggccc gccttgccca tgacgatcac
     6361 cgtcttctgc gcgtccagcg accgcatcgc cgccaagcac gtcgaggtgg cggcggagct
     6421 gggcgccgaa ctggcgcggc gggggcacac cctggtcacc ggcgggggga ccaggtcgtg
     6481 catgggcgcg gtcgccaggg cggcgcgggc cgggggaggg cggacggtcg gcgtggtccc
     6541 ggcgggcatg cccggctcgc aggagggcgc cgacaccgag aacgacgagc tggtgcgcac
     6601 gcccgacatg cgctcgcgca aggccgagat ggaccgcagg gccgacggct tcctcatcct
     6661 gcccggcggc atcggcacgc tggaggagct gctggaggtc tggacggccc gcagcctgaa
     6721 cttccacacc aagccgatcg tggtcctgga cccgtacggg gtgtacgagc cgctgcgcac
     6781 cctgatggac cacctgctcg aggagggctt caaccggccg gccgcggccg aggccgtctc
     6841 ctacaccacc gaggtgggcc aggccctcga cctgctggag tcggagtacc gcagggtgaa
     6901 cgaggaactc ctggagttct gagccgcgcg ccaggaaact tcggaagttc cggcggattt
     6961 cgcacggctc tcacagagcg ttcaacctgc cgggtcagga tccgcgaggt cggcaaaggc
     7021 cgatcatgcc tgcggcaagg agttcctgac ccatgagtgt ttcccgtcgt ggtttcgtgg
     7081 ccggtggagc cgtctccatc gcgctcgccg gaagcctgga ctccgtcttc gtgaccgcgg
     7141 ccggcgcggc cgagggcgag gcgtacggct acgggccgct ggtccccgac ccggcgggag
     7201 tcctggacct gccccgcggc ttcagctacg aggtgctgtc caccgagggc gaggcgctgc
     7261 ccggcggcgg cgtcgtgccc ggcgcgcacg acggcatggc ggccttcccc ggccgccgcc
     7321 acgacgacct gcgcctggtg cgcaaccacg agcaggagga ggacgggacc aaggccgtcg
     7381 cccgccccga gctgacctac gaccccgagg ccttcggcgg caccaccacg atccacgtgg
     7441 accgcgaggc cagggtgctg gaccagtacg tcagcctcgc cggcaccgcc gacaactgcg
     7501 cgggcggcgt caccccgtgg ggcacctggc tgacctgcga ggagaccgag ggcttcgacg
     7561 cccagaccaa gtcgcacggc tgggtgttcg aggtcgaccc gaccggccgc aggaccgtgg
     7621 ccgagccgat cgagccgctg ggccggttcg agcacgaggc cgtctgcatc gacccgcgcg
     7681 acaacgtcgt ctacctgacc gaggacgccg aggagccgtt cgggctcttc taccgcttcc
     7741 tgccccggtc gcaccgcggc ggctaccact gctaccaggc gggcggcgcc ctgcaggcga
     7801 tgcgcgttcc gggcgtctcc gacctggccc aggtgatgga ggccggcacc gtcctgcggg
     7861 gcgtgcagtg gctcgacgtg cccgacccgt cggcggcgaa gacctccacc cgcgcccagt
     7921 tcgacgacgt cacccgctcg tcgaagctgg aaggcgcctg gtggagccgg cacaaggcgt
     7981 acttcgtcgc ctcctacgcg ctcagggagg agggcgcggc cggcgaccac gcgggccagg
     8041 tctgggccta cgacccgcgc cgcaacaccc tggagctgga actggtcttc aagccgggcg
     8101 ggaggttcga gtcgcccgac aacatcaccg tctcgccgta cggcggcggc gtgatcctgg
     8161 ccgaggacgg cgacggcgag cagttcctcg tcggcaccga ccgcaggggc gagcccttcg
     8221 cgctggcccg caacgccctg aacgactccg agttcgccgg agtgacgttc tccccggacg
     8281 gccgcaccct cttcgccaac cgccaggagg gccccggcac caccttcgcc atccgcggcc
     8341 cctggcagcg gatcggcggc cgccgctgac ctctcaggcc agagcccgtc tcccgaccgc
     8401 cggggggcgg gctcccgcca tcgcggagac catgtcgagc acctggcgca gctcgtccgg
     8461 atcgccctcg tactcgaagg ccgccgctcc cagccaggcg tagacggccg ccgcggccag
     8521 ggcccccgag ccggttccgg tgacggtcac cttcaccgga cgccccccgg cgaccagggc
     8581 ctccagctct ccggcggcgg ggtcggcgcc cgcgtcgagc aggtcttccg gatccccggg
     8641 aggtccggtc cacaccagat cacggcggtt tcgcgcgttc atatcctcga gcatggcacg
     8701 atcgtcgtac cggtgaagga ggaccccgtg cccgtagtgc tcttgctcgc cgccgtcgtg
     8761 gtggtcgcgg ccgccgtcgt gctggcctcg gggcggggcg gtgagctgcg cgagcccgtc
     8821 gaggagctgc cgccgacggg gctgccggag gaccggctgc cgaccgggac ggacgcggcg
     8881 atggtgaagc tgcccaaggg ggtctggggc taccacccgc tgctcaccga cgaggtgctg
     8941 gggcggctcg tgcacgccct gatgcgccgg gagagcgagg tggccgcgct ggagcggcgg
     9001 atcgccgagc tgaccgcgta cgggccgggg gagagcctgg gcgcgtcctg gctcaagaaa
     9061 ccggacgagc cggacttcct ggacgggccg ttcgggcggg accgggcctg acggagcggc
     9121 gaaaaaggcc aggacaaggg gccatcggag atctcgcgga cctgatcgtt accacttcgc
     9181 gcccggaaca cgggataatg gaccactact gctagacgcg gacgcttcgg cggccagcgg
     9241 ctcgcacggg cccgaacggc cctgggccgg gaaggaaggg gatgcacgca tggcggcgat
     9301 gaagccgcgg acaggtgacg gtccgctcga agtgaccaag gagggacgcg gcatcgtcat
     9361 gcgcgtgccg ttggaaggcg gcggccggct cgtggtcgag ctgactcccg acgaggccag
     9421 agagctgggc gaggccatcg gcggagtggt cggctgaaga gccgcacggc cgtgcggtcc
     9481 cggcagatct cgccgccggg accgaggccg tcttcgtcct ttttcttttt cctcttccca
     9541 ccgtcgccgt ctccggcttt tctcccctag ggggccgtca tgcccgtcct gacccacctt
     9601 cgcgcggccg aggagccgct cgcccaggtg cccggcgccc gcctgctggc ggtgccccag
     9661 ggcggtgacc ccggtttcga cacgggactg ccggtggacg agctgctggc cttccacaag
     9721 gccaagggcg aggccggcga gatcgtctcc ctgccggtcc aggtcggggg agccctgcgg
     9781 gaactgctgc tgtacggcac cggcgacggc tcggcctccg cgctgcgcaa gaccggcgcc
     9841 gcgctggccc gccgcgccaa gggcgaggac gtgctggcgg tgcgcgcccc cgacggcgac
     9901 ctgacggcct tcaccgaggg cgcgctgctg gcgggctacg acttcagcat gaagaccggc
     9961 gagggcaaga agcccgtcgg ggagatcgtg ctggtcggcg gcgagccgtc ggcccggggc
    10021 gaggtgctgg ccgccgcggc cgcgctggcc cgcgacctga ccaacacccc ctcgctggag
    10081 aagtgccccg cctggctggc cgaccgggcc gcagacctgg cccgcgacgc gggccttcgg
    10141 atccgcgtcc gcgacgagcg cgaactggcg gccgagggct tcggcgggct gctggccgtc
    10201 ggcgcgggtt cgccccgccc gccccgcctg atcgagctct cctacgagcc cgagggcgcg
    10261 agccggcaca tcgtgctggt cggcaagggc atcaccttcg acaccggcgg catctcgatc
    10321 aagccgagcg ccgcgatgca gaccatgaag accgacatgg cgggcggcgc cgtcgtgctg
    10381 ggcgtcatgt cggcgc
//
