LOCUS       NZ_PVZV01000003        11812 bp    DNA     linear   CON 13-DEC-2024
DEFINITION  Actinocorallia populi strain A251 contig3, whole genome shotgun
            sequence.
ACCESSION   NZ_PVZV01000003
VERSION     NZ_PVZV01000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Actinocorallia populi
  ORGANISM  Actinocorallia populi
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Streptosporangiales; Thermomonosporaceae; Actinocorallia.
REFERENCE   1  (bases 1 to 765370)
  AUTHORS   Li,X.
  TITLE     Actinocorallia populi sp. nov., an endophytic actinomycete isolated
            from a root of Populus adenopoda (Maxim.)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 765370)
  AUTHORS   Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2018) College of Light Industry, Textile and Food
            Engineering, Sichuan University, No. 24, First South Section First
            Ring Road, Chengdu, Sichuan 610065, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04 OR MAR-2018
            Expected Final Version            :: yes
            Genome Coverage                   :: 221.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002995495.1-RS_2024_12_13
            Annotation Date                   :: 12/13/2024 14:18:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,861
            CDSs (total)                      :: 7,799
            Genes (coding)                    :: 7,716
            CDSs (with protein)               :: 7,716
            Genes (RNA)                       :: 62
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 18 of 83
            Pseudo Genes (incomplete)         :: 70 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:05:04
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 459740
            Orig. end                         :: 471552
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            PVZV01000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11812
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="3"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..11812
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11812
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:6812](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6812)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(533..548)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: strong, score: 24.89"
                     /tool="antismash"
     gene            complement(539..1747)
                     /locus_tag="C2865_RS15270"
     CDS             complement(539..1747)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF019310.5"
                     /locus_tag="C2865_RS15270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="WP_106399115.1"
                     /transl_table=11
                     /translation="MKSYLALLRTPGAWRFLLPALCARLPFAMLQLGILLLVQWATGSY
                     GWGGIASAASAVAQALLGPWTGRLADRHGQARVLVPQVLAHAVLLAVLLVLAQRRAEPL
                     LLVAVGCLAGATVPQVGSMVRARWAHLLPPRRVGTAFALESVTDELTFTLAPIVLLTVA
                     TAHSPVWALALALVLVLAGTLMFAGVRHGAPEPGPAAAGAAGAVLGRRGVPLLAVAFLA
                     VGSVFGSLQVGLTALTAELGSPGSAGTIYGTFSAGSLVGGLVYGGLKSGTRLPSRLTLL
                     LTLLAAALAGPVLVQSLGLMYGAAALTGLVIAPCIITGYTLLDALVPAEVKTEAYAWLN
                     GAVALGIALGAALSGQLVDRLGAAPAFLVPPAVVALAALLVGLRGASLRPDAAPARAPE
                     VMV"
     gene            1911..2684
                     /locus_tag="C2865_RS15275"
     CDS             1911..2684
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (49-135). E-value:
                     6.1e-10. Score: 31.0. Matches aSDomain:
                     nrpspksdomains_C2865_RS15275_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634225.1"
                     /locus_tag="C2865_RS15275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_106399116.1"
                     /sec_met_domain="Abhydrolase_6 (E-value: 2.2e-13, bitscore:
                     46.7, seeds: 455, tool: rule-based-clusters)"
                     /sec_met_domain="PF00561 (E-value: 2.2e-06, bitscore: 22.9,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAATPLTLTTSDGVRVAARHHAAGPSADAELCLVVGHGFTCHQEM
                     PGLRPIVAALGRTAAVCTLDYRGHGRSGGRSTVGDREIHDLEAAVRHARDHHARVVTVG
                     FSMGASIALRHAALIGGVDAVVSVSSPARWYYRDTVPMRRVHWAIERRLGRAAVRLAKR
                     TRVSPRGWDPVPAAPFEVVGDIACPVLIVHGGRDAFFPPDHGELLYERATRPKELWMEP
                     EMGHAEVATTEALSLRIAEWAARASAGSLPFSDAG"
     aSDomain        2058..2315
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_C2865_RS15275_Abhydrolase_1.1"
                     /evalue="6.10E-10"
                     /label="C2865_RS15275_Abhydrolase_1.1"
                     /locus_tag="C2865_RS15275"
                     /protein_end="135"
                     /protein_start="49"
                     /score="31.0"
                     /tool="antismash"
                     /translation="PIVAALGRTAAVCTLDYRGHGRSGGRSTVGDREIHDLEAAVRHAR
                     DHHARVVTVGFSMGASIALRHAALIGGVDAVVSVSSPARWY"
     gene            complement(2707..5004)
                     /locus_tag="C2865_RS15280"
     CDS             complement(2707..5004)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /GO_process="GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PPDK_N"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013491.5"
                     /locus_tag="C2865_RS15280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="WP_106399117.1"
                     /sec_met_domain="PPDK_N (E-value: 2.7e-63, bitscore: 209.6,
                     seeds: 105, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSALGGKGENLRRLAGAGLRVPAWSAVGVEVLSGFRERSGLDQEL
                     AGLLAGLDPDNAREVSARVAKAFSVTPLDGPALTAIWRAYDAVGQGTVAVRSSGADEGL
                     PNFSFAGQYSSFLGAAGFDAVAERVRDCWASAYSERSLVYRWLHDLPTDRIDLAVVLQE
                     LVPAEVSGVMHTADPVSGDRSKALISCVYGLGETLASGRVDADVLTLDRPSRVVGDAVI
                     GEKAERLDAGPGGTGLSPVSPFARERFALTNDQVGELGDLADAVEEVFGAPQDVEWALS
                     DGVFHVLQSRPITGLREAEGEVRVWDNSAVAEAFGEITAPLTFSFARHAYGRVYRDYAR
                     LLGVPRRDLEKLDGVFGELLGYFDGRVYHNVLNGYKIIRLLPGYALNRRMLAEATGVRE
                     AAEELAEAQHPIDSPLRERLVRLRVALRFLCMLFTVRRSVASFTDRFQRAYEELDAVDY
                     EELPGHEVGRALHRLEEDLLSEWGGTAVLENMIMLSYGVLHGLNARWLPDAPEWLAREA
                     VKVGDDVESAAPARRLGELAGHLAERPGLADEVRERPPGELWEWLREAEGEDAGWLRAE
                     LDGFLAAFGHRSAGGLRLEEPDLHDDPSLLMSMLKDALLLSGLAPASGEEHDVEGYLRE
                     HLDGPRRFVYDLVRGRVREALRERERVRFARSRAFGMARRMFRAIGRDLASVGALGDRR
                     DVFQLKLEEIRAAYAGTMNHRELRPLAELRKTQQAKQRLMPAPPPRFLTRGSVYWGVRT
                     ADPAPAGEGRRG"
     gene            complement(5001..6812)
                     /locus_tag="C2865_RS15285"
     CDS             complement(5001..6812)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF015700.5"
                     /locus_tag="C2865_RS15285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AfsA-related hotdog domain-containing protein"
                     /protein_id="WP_106399118.1"
                     /sec_met_domain="AfsA (E-value: 1.1e-17, bitscore: 59.3,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAVARLGEAQAEVPEATIGGEHAEPVPEALSALPRKRALFSVDG
                     YFRDGWDDLLAVASDPWTLLDRHAMLLLTPAAVAGRRLEAALEWLLEQDAVVVAAEPVT
                     LDRHSTRAMWWYRWNAATRRRRELTEALARAGDSLLLAVRLPEAPVPATLRLNDLKGPA
                     DPARREPWQLRHRLDDGGSPVDLVHTADEPADLVREFGILLDRRDRRRVYADLVAGADR
                     EAGTRALIEELYRRSPGHDLSRDRARRRRALGEPDEPWDSVVLGAAAADPAAPGGTALL
                     PGVTAAQWEHAAARGPRPLRRERALPGPLVHKDGLGEVFLTDHVATGSGGFLMAGELPA
                     AHRFYNDLPSARLDLLPVLELCRQGCYVVAHGHHGVPQDARFVLEELSGRLTADPEAVR
                     DRRVRVEADVVRRSASAMALDYRVTAPDGTGLATARASFSWRDEAGWRGLRAAGRARHG
                     LGGETGAPDRTGLFPSARNVGRTHDGNVVLADLAWRRGESTARVAADPANPAMFERPQD
                     HVPDMVLLEAARQAALWTMSRHFGVPAGRLAVTGFTTRFAAMAELDVRLACTATVSADR
                     GGKGAVAVRFVQDGVEPAEVMVEVVRA"
     misc_feature    6995..7008
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.07"
                     /tool="antismash"
     misc_feature    complement(6997..7010)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.05"
                     /tool="antismash"
     misc_feature    complement(7002..7017)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.68"
                     /tool="antismash"
     misc_feature    complement(7003..7012)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 12.92"
                     /tool="antismash"
     misc_feature    complement(7030..7043)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.42"
                     /tool="antismash"
     misc_feature    complement(7067..7084)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.38"
                     /tool="antismash"
     misc_feature    7174..7189
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.36"
                     /tool="antismash"
     gene            complement(7325..8221)
                     /locus_tag="C2865_RS15290"
     CDS             complement(7325..8221)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852995.1"
                     /locus_tag="C2865_RS15290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_106399119.1"
                     /transl_table=11
                     /translation="MARAADATVLNISAAAGGGPTVLRILLGAQLRRLREDQGITREQA
                     GYAIRASDSKISRLELGRVSFRERDVADLLTLYGVHDPAEREKALALARRASANGWWHD
                     YNDLLPRWFENYIGLEEAASRIRAYEVQFVPGLLQSESYARAVTLLGHGGAAVEDVERR
                     VEMRMKRQELLHSDHAPHLWAVVDEAVVRRPLGGTGVMREQLRHLIELCELPNVTVQVV
                     PFARGGHAAAGGPFSILRFAEPDLPDIVYLEQLTSALYLDKREDIERYLAVMEQLCVEA
                     EPVTATPDLLKRIHDTM"
     misc_feature    8263..8276
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.94"
                     /tool="antismash"
     gene            complement(8415..8648)
                     /locus_tag="C2865_RS15295"
     CDS             complement(8415..8648)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017627179.1"
                     /locus_tag="C2865_RS15295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF397 domain-containing protein"
                     /protein_id="WP_198653337.1"
                     /transl_table=11
                     /translation="MGDHAQGAFAHAAEPLWFKSRHSNPSGNCVELTALPSGEIAMRNS
                     RHPDGPVLIYTRAEIEAFLGGAKDGEFDHLVA"
     misc_feature    8669..8678
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.26"
                     /tool="antismash"
     gene            8787..9311
                     /locus_tag="C2865_RS15300"
     CDS             8787..9311
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024970.5"
                     /locus_tag="C2865_RS15300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ATP-binding protein"
                     /protein_id="WP_110554877.1"
                     /transl_table=11
                     /translation="MTVKRAPRMINSRRLFCTDEGRWPEMLPTLHASNWPTPPVSGTSR
                     LARHPLNSNLDSPRIARDFARKTLAEWELEDLLCEAGVVISELVTNALRYGLPGRHRIA
                     RAPIQLILMRQERRVLVIVTDPSGEPPVLGEPDEFAESGRGLQVVAAVSEAWSWAPLST
                     GGKAVWASFAL"
     misc_feature    9347..9361
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.74"
                     /tool="antismash"
     gene            9386..10669
                     /locus_tag="C2865_RS15305"
     CDS             9386..10669
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1202: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF019310.5"
                     /locus_tag="C2865_RS15305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="WP_198653338.1"
                     /transl_table=11
                     /translation="MRAEGRTSASGLPAVYWYLWLGMLINRVGGFGVLFLSLYLVQRRG
                     LDASAAGLVVGLSGAGGAVGVLAGGVLADRWGRRRTLLACHLLAAGLMLALAFATRLPV
                     IAAVTFLLGAFQSMAGPPLVAAITDVVPPPDRQRAFNLQFWAHNLGVAAAALLAGLLAE
                     ASFTLLFVVEAAATFATFLLLAVKVPETLPAARGGRSPAERGSGLGAVLTDRVYLVFVG
                     LTLLLAYVSTQSSTILPLAMSADGLGPAAYGLVTGCAGALIVAGQLLVPRFIAGRPKAR
                     VLALANLLLGGGFALVALADRVPLHLAAALVWTLGSMLAAPPNAEVIAELAPERLRGRY
                     QGVFYLVFPIASFAAPALGGVSLERLGDLHWVLCGAVGALAALGHLLAGPARERRAALG
                     PKQSVSEWYMNDRGRSSERSSFHGCEGT"
     gene            10698..11573
                     /locus_tag="C2865_RS15310"
     CDS             10698..11573
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="C2865_RS15310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_106399122.1"
                     /transl_table=11
                     /translation="MNVGGPVRAAEARAVKGAAARRDTRDFQRTPLPDPGGAFLLRAVC
                     DPYAVGATGTGPLILHWNGTLWEKEPLPAFDPALSGAMLTGVAGRVAVGGALDRLTGAE
                     TPLLLCRDDTGWVEEKAPGTGFPYVLTGVHGDWAAGHGFPGTVLLHRGLGGWTHLQTPG
                     RPARLLAVASLGRHVWAGGERDREGLLLSFDGRSWKEHRTRTGPVTALAIRNGRPWAAA
                     GRTLLEWTGRRWARHTAPLDVNALSTTASGALCAAGAGALGVHDGRRWDLRALPGTWLG
                     ADPVWLVGSA"
ORIGIN
        1 catgccgccg acgaccaccc gggtgagcac ccgcggcccg tcgaccgaga cgacctccag
       61 ggagaccttc ccgtcgtcga tgagcacggt gtcgccggag gagacgtcgc cgggcaggcc
      121 cttgtaggtg gtggagacct ccttgcggct gccggggacg tcctgggtgg tgatggtgaa
      181 ctcatcgccc acgttgagcc gcaccggccc gtcggggaag cggcccagcc ggatcttggg
      241 gccctgcagg tcggccagca cgccgacggc gcggccggtg gcctcgcccg cctcgcgcag
      301 gtggtggtag acctgcgcgt gctgctcgtg cgacccgtgg ctcatgttca gccgggccac
      361 gtcgatcccg gcctccacga gggccttgat ctgctcgcgg ctggaacacg ccggtccgat
      421 ggtgctgacg atcttcgctc gacgactcac gtgttccacc ctaggtagtt acccgccggt
      481 acgcgcacct tcccggccgg gcatcaccgt ctgttcaccg agaacttccc tcagtggtct
      541 agaccatgac ttccggggcg cgcgccgggg cggcgtccgg gcgcaggctc gcgccgcgca
      601 gccccacgag cagggccgcc agcgcgacga cggccggcgg gacgaggaag gccggggcgg
      661 cgcccagccg gtcgacgagc tggcccgaca gggcggcgcc cagggcgatg cccagcgcca
      721 ccgccccgtt cagccaggcg taggcctcgg tcttcacctc ggccggcacc agcgcgtcca
      781 gcagggtgta cccggtgatg atgcaggggg cgatgaccag gccggtcagt gccgccgccc
      841 cgtacatcag gcccagggac tgcaccagga cgggtccggc gagcgcggcg gccagcaggg
      901 tcagcagcag ggtcagccgg gacggcagcc gggtcccgga cttcagcccc ccgtagacga
      961 gcccgccgac gaggctgccg gccgagaacg tgccgtagat ggtgccggcc gagccggggc
     1021 tgcccagctc ggcggtcagc gcggtgaggc ccacctggag gctgccgaag accgagccga
     1081 cggccaggaa ggcgaccgcc agcagcggca cgccccgcct gcccagcacc gctccggccg
     1141 cgcccgccgc cgcggggccg ggttcggggg cgccgtgccg gacgccggcg aacatcaggg
     1201 tgcccgccag caccaggacg agcgccagcg ccagggccca gacggggctg tgggcggtgg
     1261 cgaccgtcag cagcacgatg ggggccaggg tgaaggtcag ctcgtcggtg accgactcca
     1321 gcgcgaaggc cgtgcccacc cgccggggcg gcaggaggtg cgcccagcgg gcccggacca
     1381 tcgagccgac ctgcggcacc gtcgcgcccg ccaggcagcc cacggccacc agcagcagcg
     1441 gttcggcccg ccgctgggcc agtaccagca gcacggccag caggacggcg tgcgccagca
     1501 cctggggcac cagcaccctg gcctggccgt gccggtcggc cagccgcccg gtccacgggc
     1561 cgagcagcgc ctgggccacg gccgaggcgg ccgaggcgat gccgccccag ccgtaggagc
     1621 cggtggccca ctggacgagc agcaggatgc ccagctgcag catcgcgaag ggcagccggg
     1681 cacagagcgc gggcagcagg aagcgccagg caccgggggt gcgcagcagc gcgaggtagg
     1741 acttcaagat ccgggttctc cgtcgtcgag tcgtgacgga tcccggccac cccggtccgc
     1801 caacagtttc agcgtcggac cggggtagat accgcccttt atcaggacag ccgccatgcg
     1861 gtcagaagtg cgagtttata cccggcctga acaggaccga taatgggagc gtggccgcca
     1921 ctcccctcac tctcaccacg tccgacggcg tccgggtcgc cgcccgccac cacgcggcgg
     1981 gcccctccgc cgacgcggag ctgtgcctgg tggtcgggca cggcttcacc tgccaccagg
     2041 agatgcccgg cctgcggccg atcgtcgcgg cgctgggccg caccgccgcg gtctgcacgc
     2101 tcgactaccg ggggcacggg cgctcgggcg ggcgctcgac cgtcggcgac cgggagatcc
     2161 acgacctcga ggcggccgtc cgccacgcca gggaccacca cgccagggtg gtcaccgtcg
     2221 ggttctccat gggcgcctcg atcgcgctgc ggcacgccgc cctgataggc ggcgtcgacg
     2281 cggtggtgtc ggtcagctcg ccggcccgct ggtactaccg ggacaccgtg cccatgcggc
     2341 gggtgcactg ggccatcgag cgcaggctgg gccgcgccgc cgtgcggctg gccaagcgca
     2401 cccgcgtctc cccccggggc tgggacccgg tgcccgccgc gcccttcgag gtggtcgggg
     2461 acatcgcctg cccggtgctg atcgtccacg gcggccgcga cgcgttcttc ccgcccgacc
     2521 acggcgaact gctctacgag cgggccaccc ggcccaagga gctgtggatg gagcccgaga
     2581 tgggccacgc cgaggtcgcc accaccgagg cgctgagcct gcgcatcgcc gagtgggccg
     2641 cccgcgcgtc ggccgggtcc ctgcccttct ccgacgcggg ttgaggcggg cggtctccgg
     2701 tccgcctcaa ccgcgccgcc cctcccccgc cggggccgga tcggcggtcc gcacgcccca
     2761 gtagacgctg ccgcgggtga ggaacctcgg cggcggcgcg ggcatgagcc gctgtttggc
     2821 ctgctgggtc ttgcgcagct cggccagcgg gcgcagctcg cggtggttca tggtcccggc
     2881 gtaggcggct cggatctcct cgagcttgag ctggaagacg tcgcggcggt cgcccagcgc
     2941 gccgaccgac gccaggtccc tgccgatggc cctgaacatg cggcgggcca tgccgaacgc
     3001 gcgggagcgg gcgaagcgga cgcgctcgcg ttcgcgcagc gcctcgcgca ccctgccgcg
     3061 caccaggtcg tagacgaacc gccgggggcc gtccaggtgc tcgcgcaggt agccctcgac
     3121 gtcgtgctcc tccccggagg ccggggcgag gccgctcagc agcagggcgt ccttgagcat
     3181 ggacatcagc agcgacgggt cgtcgtgcag gtccggctcc tccagcctga gtccgccggc
     3241 gctgcggtgg ccgaacgcgg cgaggaagcc gtcgagttcg gcgcgcagcc acccggcgtc
     3301 ctcgccctcg gcctcgcgca gccactccca cagctcgccc ggcggacgct cgcgcacctc
     3361 atcggccagg ccggggcgct cggccaggtg cccggccagc tcgccgagcc gccgcgcggg
     3421 cgccgcggac tcgacgtcgt cgccgacctt caccgcctcc cgcgccagcc attcgggggc
     3481 gtcgggcagc cacctggcgt tcagcccgtg gagcacgccg tacgagagca tgatcatgtt
     3541 ctcgagcacg gcggtgccgc cccactccga caggaggtcc tcctccaggc ggtgcaaggc
     3601 gcggccgacc tcgtggccgg gcagctcctc gtagtcgacc gcgtcgagct cctcgtacgc
     3661 ccgctggaag cggtcggtga aggacgcgac cgaccggcgg acggtgaaga gcatgcacag
     3721 gaaccgcagg gcgacgcgca gccgcaccag gcgctcccgc agcggggagt cgatcgggtg
     3781 ctgggcctcg gcgagctcct cggccgcctc cctgacgccc gtcgcctcgg cgagcatgcg
     3841 ccggttgagc gcgtaccccg gcagcagccg gatgatcttg tacccgttga ggacgttgtg
     3901 gtagacgcgg ccgtcgaagt agcccagcag ctcgccgaac accccgtcca gcttctccag
     3961 gtcgcggcgc ggcacaccca gcaggcgcgc gtagtcgcgg tagacgcggc cgtaggcgtg
     4021 acgcgcgaag ctgaaggtca gcggggcggt gatctcgccg aacgcctcgg cgacggcgga
     4081 gttgtcccac acccgcacct cgccctcggc ctcgcgcagg ccggtgatcg ggcggctctg
     4141 cagcacgtgg aagacgccgt cgctcagcgc ccactcgacg tcctgcggcg cgccgaagac
     4201 ctcctcgacg gcgtccgcga ggtcgcccag ctcgccgacc tggtcgttgg tcagcgcgaa
     4261 ccgctcgcgg gcgaacgggg agaccgggct gagccccgtg ccgccgggcc cggcgtcgag
     4321 ccgctcggcc ttctccccga tgacggcgtc gccgacgacc cggctcggcc ggtccagggt
     4381 gaggacgtcg gcgtcgaccc ggccggacgc cagcgtctcc cccagcccgt agacgcagct
     4441 gatgagggcc ttggagcggt cgccggagac cgggtcggcg gtgtgcatca ccccgctcac
     4501 ctcggcgggg accagctcct gcagcacgac cgccaggtcg atccggtcgg tgggcaggtc
     4561 gtgcagccag cggtagacca gggaccgctc cgagtacgcc gaggcccagc agtccctgac
     4621 ccgctcggcc accgcgtcga acccggccgc gcccaggaac gaggagtact gcccggcgaa
     4681 ggagaagttc ggcaggccct cgtcggcgcc cgaggagcgc accgcgacgg tgccctggcc
     4741 gacggcgtcg taggcgcgcc agatcgccgt cagcgcgggg ccgtccagcg gtgtcaccga
     4801 gaaggccttg gcgacgcgtg ccgagacctc ccgggcgttg tcggggtcga ggccggccag
     4861 caggcccgcc agctcctgat ccaggcccga acgctcccgg aacccgctca ggacctccac
     4921 cccgaccgcc gaccacgccg ggaccctgag ccccgccccg gccagccgcc gcaggttctc
     4981 tcccttgccg ccgagcgcgc tcatgcccgt accacctcca ccatcacctc cgccggttcc
     5041 accccatcct ggacgaacct cacggccacc gcgccctttc caccgcgatc ggcggagacc
     5101 gtcgcggtgc aggcgagccg gacgtccagc tccgccatgg ccgcgaagcg ggtggtgaag
     5161 ccggtcacgg ccagccggcc ggcgggcacg ccgaagtggc gggacatggt ccacagcgcg
     5221 gcctggcggg cggcctccag cagcaccatg tccgggacat ggtcctgcgg gcgctcgaac
     5281 atcgccgggt tcgccgggtc cgccgcgacc cgcgcggtgc tctcgccgcg ccgccacgcc
     5341 aggtcggcca gcacgacgtt gccgtcatgg gtgcgcccga cgttgcgggc ggaggggaac
     5401 aggccggtcc ggtcgggggc gccggtctcc ccgccgagcc cgtgccgggc gcggcccgcg
     5461 gcgcgcagcc cgcgccagcc cgcctcgtcc cgccaggaga aggacgcgcg ggcggtggcc
     5521 agcccggtcc cgtcgggggc ggtgacccgg tagtccaggg ccatcgcgga ggcggaccgc
     5581 cggacgacgt ccgcctcgac ccgcacccgc cggtcgcgga cggcctcggg gtccgcggtg
     5641 agccgcccgc tcagctcctc cagcacgaag cgggcgtcct gcggcacgcc gtggtggccg
     5701 tgggcgacga cgtagcagcc ctggcggcac agctccagga cgggcaggag gtcgagcctg
     5761 gccgagggca ggtcgttgta gaagcggtgg gcggcgggga gctctccggc catcaggaag
     5821 cctccggagc cggtggccac gtggtcggtg aggaagacct ccccgaggcc gtccttgtgc
     5881 accagggggc cggggagggc gcgttcgcgg cgcagcgggc gcggaccgcg ggcggcggcg
     5941 tgctcccact gggcggcggt gacgccgggg agcagcgcgg tcccgccggg cgccgccggg
     6001 tccgcggcgg cggcgccgag caccaccgag tcccagggtt cgtccggctc gccgagcgcc
     6061 ctgcgccttc gggcgcggtc gcgggacagg tcgtgcccgg ggctgcgccg gtacagctcc
     6121 tcgatcagcg cgcgcgtccc tgcctcccgg tcggcccccg cgaccaggtc ggcgtagacg
     6181 cggcgccggt cgcggcggtc cagcaggatg ccgaactcgc ggaccaggtc ggcgggctcg
     6241 tcggcggtgt gcacgagatc gaccggggag ccgccgtcgt ccagccggtg ccgcagctgc
     6301 cacggttcgc ggcgggccgg gtcggccggg cccttgagat cgttcagccg cagcgtcgcc
     6361 gggaccggcg cctcgggcaa ccgcaccgcc agcagcaggc tgtcgcccgc gcgggccagc
     6421 gcctcggtca gctcccgccg ccgcctggtg gcggcgttcc accggtacca ccacatcgcg
     6481 cgggtgctgt gccggtcgag cgtgacgggc tcggccgcga cgaccaccgc gtcctgctcc
     6541 agcagccact ccaaagccgc ctccagccgc cgcccggcca ccgcggcggg ggtgagcagc
     6601 agcatggcgt ggcggtccag cagcgtccac gggtcggacg cgaccgcgag caggtcgtcc
     6661 cagccgtcgc ggaagtagcc gtcgaccgag aacagcgcac gttttcgcgg cagcgcggag
     6721 agcgcctcgg gaaccggttc ggcgtgttcg ccgccgatcg tcgcctccgg gacctccgcc
     6781 tgcgcctcgc cgagcctggc gaccgccgtc atgccacctc tccctgccgc gcgatgatct
     6841 tcagttctcc ggccggcggc cgccggttct cgccgccggc cgtctccgat ggcccgggag
     6901 cgtcgccgcg caggagccgg cgggtccgcg gccgccggag ggccgcgcgg gggcgccgcc
     6961 gctccccgcc ggccgccccc cgtgctttac cgacagttca tgtgaaaaac catcgaagac
     7021 gagtggatct ttggttgtca aaccagaacg agaagggcac ccggtgttca cctgacttta
     7081 acaggggttc cgagatagga gtgtcccagg tcacagcggc tgccagagcc cttgccccac
     7141 gggcggagga ggagggtcaa tacggcccgg ctttgtcaga tgggtcacaa tcagagcccg
     7201 cggcgagaga attcgcaccg cgaattaaag gttcgccgcc cgcccgcgtc cgccgccgga
     7261 ggaaacaaag aacgcccggc cccaggcgga acctggagac cggacgttcc ggcgggctcc
     7321 gacttcacat cgtgtcgtgg atgcgcttga ggaggtccgg ggtggcggtg accggctcgg
     7381 cctccacgca cagctgctcc atgaccgcga ggtagcgttc gatgtcctcg cgcttgtcga
     7441 ggtacagggc gctggtgagc tgctcgaggt agacgatgtc cggcaggtcg ggctcggcga
     7501 agcgcaggat gctgaagggc ccgccggccg ccgcgtgccc gccccgggcg aacggcacga
     7561 cctggacggt gacgttgggc agctcgcaca gctcgatgag atgccgcagc tgctcgcgca
     7621 tcacccccgt gccgcccagc gggcgccgga cgaccgcctc gtcgaccacg gcccacaggt
     7681 ggggcgcgtg gtcggagtgc agcagttcct ggcgcttcat acgcatctcg acccggcgct
     7741 cgacgtcctc cacggccgcg ccgccgtgac ccagcagcgt gaccgccctg gcatagctct
     7801 cgctctgcag caggccgggg acgaactgca cctcgtaggc gcggatgcgg ctggccgcct
     7861 cctccaggcc gatgtagttc tcgaaccagc gggggagcag gtcgttgtag tcgtgccacc
     7921 acccgttggc gctggccctg cgggccagcg cgagcgcctt ctcccgctcg gcggggtcgt
     7981 ggacgccgta gagggtgagc aggtcggcca cgtcgcgctc gcggaaggag acccgtccga
     8041 gctccaaccg gctgatcttg gagtccgagg cgcggatcgc gtagcccgcc tgctcgcggg
     8101 tgatgccctg gtcctccctc agacgccgca gctgcgcacc cagcaggatg cgcagcacgg
     8161 tggggccgcc gccggccgcg gcggagatgt tcaacacggt cgcgtctgcc gctcgggcca
     8221 ttcggggctc cctgcggacc tgccttcccg gctcggccct atgagacttc ctataacggg
     8281 cgaatcagta tcccatgcgg cacctctctt ggccatgcgc tccccgagac ccgccctccg
     8341 taaccccagg tgaccgcggg tcagccccct ggtcaggcgc cccgggacgg cacggccgcc
     8401 ggttgagcga tcgctcaggc gacgaggtga tcgaactcac cgtccttggc gccgccgagg
     8461 aacgcctcga tctccgcgcg ggtgtagatg agcaccggac cgtcggggtg ccgggagttg
     8521 cgcatggcga tctccccgct cggcagcgca gtgagctcga cgcagttacc gctggggttg
     8581 ctgtgcctgc tcttgaacca gagcggttct gctgcgtgtg cgaacgctcc ctgtgcgtga
     8641 tcccccatgg tttgctccgt tccaagcatg attttttccg ctgcaaggac cgatgcacgt
     8701 gcagaagccc ttgcacctgc acggctcgga agggaaagat agcggacacc cacgcagcgc
     8761 atcaccggac cgcgtcagga catttcatga ccgttaagcg ggctcctcgc atgatcaaca
     8821 gccgccggct cttctgcacc gacgaaggca gatggcccga gatgctgccg accctgcatg
     8881 cgagcaactg gcctaccccg ccggtctccg gcaccagccg gctcgcccgg cacccgttga
     8941 acagcaacct ggactcgccc aggatcgccc gggacttcgc ccgcaagacc ctggccgagt
     9001 gggagctgga ggacctcctg tgcgaggcgg gagtggtgat ctccgagctg gtcaccaacg
     9061 ccctgcgcta cggcctgccc ggccggcacc gcatcgcccg cgccccgatc cagctcatcc
     9121 tcatgcgcca ggagcgccgc gtcctggtga tcgtcaccga ccccagcggg gagcccccgg
     9181 tgctcggcga gcccgacgag ttcgccgaga gcggccgggg cctgcaggtg gtcgccgcgg
     9241 tctccgaggc gtggagctgg gcgccgctgt ccaccggggg caaggccgtc tgggcgtcgt
     9301 tcgcgctctg agccccccgc ccttccgcgg ccgacccggc acccctacta tctagaaagt
     9361 ttctttacag gtagcagggg tgccggtgcg ggccgagggt cggacgagcg cgagcgggct
     9421 gcccgccgtg tactggtatc tctggctggg catgctgatc aaccgcgtcg gcggtttcgg
     9481 ggtgctcttc ctgtcgctct acctcgtgca gcggcggggc ctggacgcct ccgccgcggg
     9541 actggtcgtc gggctgagcg gagcgggcgg cgccgtcggg gtgctggcgg gcggggtgct
     9601 cgccgaccgg tggggacggc gccgcaccct gctggcctgc cacctgctcg ccgccgggct
     9661 catgctcgcc ctcgccttcg ccacccggct tccggtgatc gccgcggtga cgttcctgct
     9721 cggggccttc cagagcatgg cggggccgcc cctggtcgcc gcgatcacgg acgtcgtgcc
     9781 gccccccgac cggcagcgcg cgttcaacct ccagttctgg gcgcacaacc tgggcgtggc
     9841 cgccgccgcg ctcctggccg ggctgctggc cgaggcgagc ttcaccctcc tcttcgtggt
     9901 cgaggccgcc gccaccttcg ccaccttcct cctcctggcg gtgaaggtcc ccgaaacgct
     9961 gccggccgcc cgcgggggcc gctcccccgc cgaacgcggc tccggcctgg gcgcggtcct
    10021 cacggaccgg gtctacctcg tcttcgtcgg gctgacactg ctgctggcgt acgtgagcac
    10081 gcagagctcc acgatcctcc cgctggcgat gagcgcggac ggcctcggcc ccgcggccta
    10141 cgggctggtc accgggtgcg cgggagcgct gatcgtggcc gggcagctgc tcgtgccccg
    10201 gttcatcgcg ggacggccca aggcgcgggt gctggcgctg gccaacctgc tgctgggcgg
    10261 cgggttcgcg ctggtggccc tggccgaccg ggtgcccctg cacctggcgg cggcgctggt
    10321 ctggacgctc gggtcgatgc tcgccgcccc gcccaacgcc gaggtcatcg ccgagctggc
    10381 accagaacgg ctgcgcggac gttatcaggg tgtgttctac ctggtgttcc cgatcgcctc
    10441 tttcgcggcg cccgccctgg gcggggtgtc cctggagcgg ctcggcgacc tgcactgggt
    10501 attgtgcggg gcggtcgggg cgctggcggc gctgggacac ctgctggcgg gcccggccag
    10561 ggagcggcgg gccgccctcg gaccgaaaca atccgtctcg gagtggtaca tgaatgatcg
    10621 gggtagaagc tcagaaaggt catcgtttca cggctgtgag gggacatgac cctttcgtct
    10681 tcctatggtt tcgcggcatg aacgtcgggg gaccggtccg cgcggcggag gccagggcgg
    10741 tcaagggcgc ggcggcacgc cgcgacaccc gggacttcca gcgcaccccg ctgcccgacc
    10801 cgggaggggc cttcctgctc cgggcggtat gcgatccgta cgccgtggga gccaccggca
    10861 caggaccgct catcctccac tggaacggga cgctctggga gaaggagccc ctgcccgcct
    10921 tcgacccggc cctgagcggc gcgatgctca ccggcgtggc gggacgcgtc gccgtcggcg
    10981 gggccctcga ccggctcacc ggcgccgaga caccgctgct gctgtgccgc gacgacaccg
    11041 gatgggtcga ggagaaggcg ccgggaaccg gattccccta cgtgctgacc ggcgtgcacg
    11101 gcgactgggc cgcggggcac gggttcccgg gcaccgtcct cctgcaccgc ggactgggcg
    11161 gctggacgca cctgcagacc cccggccgcc ccgcgcggct gctggccgtc gcgtcactcg
    11221 gccggcacgt ctgggccggc ggggaacgcg accgtgaagg gctgctgctc tccttcgacg
    11281 ggcggtcctg gaaggagcac cggaccagga cggggcccgt caccgccctc gcgatccgca
    11341 acggacggcc ctgggccgcg gcgggccgga cgctcctgga gtggacggga cgccgctggg
    11401 cccgccacac cgcccccctg gacgtcaacg cgctgtccac caccgcctcc ggcgcgctgt
    11461 gcgcggccgg cgcgggagcg ctgggcgtcc acgacgggcg gcgctgggac ctgcgcgccc
    11521 tgccggggac ctggctgggc gccgaccccg tctggctcgt gggctccgcc tgagggcgtg
    11581 tgatccccgc ctcccggggt tttccccacc tcgttccggc gggtagggct gacggaacta
    11641 cgatcgtttc cgatgacccc tccggacagg agacccatga cccgcgccgt ctacctcgcc
    11701 gccaccgggg ccgccacccg caaggcgtcc gtcaccctcg ggctggccga gctcctgtcc
    11761 gggaaggtcc gcaggctggg cgtgtaccgg ccggtcgtgg agtccgggcg cg
//
