LOCUS       NZ_PVZV01000002        10819 bp    DNA     linear   CON 13-DEC-2024
DEFINITION  Actinocorallia populi strain A251 contig2, whole genome shotgun
            sequence.
ACCESSION   NZ_PVZV01000002
VERSION     NZ_PVZV01000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Actinocorallia populi
  ORGANISM  Actinocorallia populi
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Streptosporangiales; Thermomonosporaceae; Actinocorallia.
REFERENCE   1  (bases 1 to 1301122)
  AUTHORS   Li,X.
  TITLE     Actinocorallia populi sp. nov., an endophytic actinomycete isolated
            from a root of Populus adenopoda (Maxim.)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1301122)
  AUTHORS   Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2018) College of Light Industry, Textile and Food
            Engineering, Sichuan University, No. 24, First South Section First
            Ring Road, Chengdu, Sichuan 610065, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04 OR MAR-2018
            Expected Final Version            :: yes
            Genome Coverage                   :: 221.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002995495.1-RS_2024_12_13
            Annotation Date                   :: 12/13/2024 14:18:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,861
            CDSs (total)                      :: 7,799
            Genes (coding)                    :: 7,716
            CDSs (with protein)               :: 7,716
            Genes (RNA)                       :: 62
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 18 of 83
            Pseudo Genes (incomplete)         :: 70 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:05:04
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1020289
            Orig. end                         :: 1031108
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            PVZV01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10819
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="4"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10819
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10819
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5819](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5819
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(73..86)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 17.38"
                     /tool="antismash"
     gene            151..567
                     /locus_tag="C2865_RS11770"
     CDS             151..567
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="C2865_RS11770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_106398376.1"
                     /transl_table=11
                     /translation="MKILINLGRGASVTVAPGRRQSLVFDKEWNDPDKVHKDGAHADWL
                     PKESAYHLATLTVIADGQADGEKLKLTITEYERDKDTRTKDALGEDKVGNGTRVEYTLS
                     GLVWMDEKRRYRAEVENFGTVPVTVEKAYLKVAR"
     misc_feature    152..166
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.98"
                     /tool="antismash"
     gene            complement(595..1299)
                     /locus_tag="C2865_RS11775"
     CDS             complement(595..1299)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630858.1"
                     /locus_tag="C2865_RS11775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_106398377.1"
                     /transl_table=11
                     /translation="MNRRENGLSADAYAPLIDLVPELADRLLTVLREAGIAAYALPGGG
                     DGPDVLDHVHVDAEHKGEAERLLHVSVLRDRVAPPPERDEDAIFAEIVAGFGETPTDVS
                     WPDEENLGPADAGDDLQDGSGRLPTARVVYYSEGPVGPVKEDGDFPEPDEEGHYEPPPP
                     PPLPTGDPASRLAWLCLVGGPLYILATVVFRWSAPGWAAFLAVAAFIGGFVILVLRMGD
                     EPRDGDDDGAVI"
     gene            1472..2191
                     /locus_tag="C2865_RS11780"
     CDS             1472..2191
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1141: acyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853370.1"
                     /locus_tag="C2865_RS11780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="WP_106398378.1"
                     /transl_table=11
                     /translation="MFWWVVKAIVGPLLVLIWRPKNDGMENVPEEGPVILASNHLSFAD
                     HFFGPWPLRRRIVFLGKRDYFTGKGVKGALSRMFFSGVGVIPIDRTGGKASEEALATGL
                     RVLREGKLLGIYPEGTRVPDNRLFKGKTGVARLALQGRVPVIPMAMMHTFELMPAGSRP
                     RVRGLPGRPGVRFGEPMDFSQYYGREDDREVLRLITDKIMMAIRELSGQEYVDRYAAEV
                     KAELAGKAVVRRDDDEE"
     gene            2188..3306
                     /gene="macS"
                     /locus_tag="C2865_RS11785"
     CDS             2188..3306
                     /GO_function="GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="macS"
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887058.1"
                     /locus_tag="C2865_RS11785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MacS family sensor histidine kinase"
                     /protein_id="WP_198653187.1"
                     /transl_table=11
                     /translation="MNVEAALWRALAVFRIVACLYAAVLIASNHQSYLHPAGGWIVLAV
                     MVAWTAFTVRAYAGGGAGPVLAGLDLVVSTGCLLATGLVETAQHLADGRPNLTASWVAI
                     PVLVWALLGGVRWGAAAALLVGIPDMLLRWLYGGEFQQTTLNGLVLLLMGGVVVGYLGR
                     LARYSQERLARAVALETATRERERLARDLHDSVLQVLALVRRRGTELGGEAAELGRLAG
                     EQEVALRALISRPAGEEEPGGERDLRDLLDRYASSSVQVSAPATPVRLPGHAAAELAAA
                     VGAALDNVSRHCGPDARAWVLVEDDGETVTVSVRDDGPGIPEGRMEAAAKGGRMGLARS
                     IKGRVRDLGGTVEIFSSPGAGTELELIVPRPR"
     gene            3385..4035
                     /locus_tag="C2865_RS11790"
     CDS             3385..4035
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017622295.1"
                     /locus_tag="C2865_RS11790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="response regulator transcription factor"
                     /protein_id="WP_106398712.1"
                     /transl_table=11
                     /translation="MTGIRVMVVDDHPMWREAVARDLAEAGYEVVATAGEGRKAVRIAA
                     AARPQVAVVDLQLPDISGVQVARSLVAADPSVRVLVLSASGEQADVLEAVKAGATGYLL
                     KSAAREEFLDAVARTAQGDAVFTPGLAGLVLGEYRRLAAAPAPEPDAPRLTDRETEILR
                     LVAKGLTYKQIAERLVLSHRTVQNHVQNTLGKLQLHNRVELVRYAIEKGLDGE"
     misc_feature    complement(4075..4090)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.65"
                     /tool="antismash"
     gene            4120..5004
                     /locus_tag="C2865_RS39330"
     CDS             4120..5004
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="C2865_RS39330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_198653189.1"
                     /transl_table=11
                     /translation="MTDSTTTWGINGPAFLGVFALVAAAAWLLGLAMRRRLRAGRAPSR
                     ELHPYELAYLQGGARLVVASVLAALRMSGSVTARPGGSLRAEGTPVAARSPLDQAVLKA
                     AGRAVRTGELADHSLLRPRLDELRDGLRAEGLLLGPEDRRVVRISALPLLFVLLVGLAR
                     MAAGLQNGKPVGYLIALLVVAAAVLAFMLYVPEKTRAVDGVLLEARTRHPELQPTASPD
                     WRSHGPEAAALGVALFGVGALVAMDPQFAEEAEIQQRLGQTGSGGGDGGGGGDGGDGGG
                     GGGGCGGCGGCGG"
     gene            5001..5819
                     /locus_tag="C2865_RS11800"
     CDS             5001..5819
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /locus_tag="C2865_RS11800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_106398714.1"
                     /sec_met_domain="DUF692 (E-value: 5.5e-69, bitscore: 228.5,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSPGVGLGWRPPLARFAADLPGLAGSGFAEVIAETLHRPDPALL
                     ELRDRGARIVPHGVRLSLGGAAPVEPERVAHLAHCAELLGAPLVSEHVAFVRAGGLEAG
                     HLLPVPRSRAALDALTRNIRLTRAELDVPLAVEPIAALFDWPDDEYTEGDFLTELLDRT
                     GALLLLDVANVYANARNRGRDPVKELESFPLDRVAYCHVAGGGEHDGYYHDTHTHALPE
                     EVLALVGEAGRRTTAGLGYLLERDGNYPPAAALRAELDAIADAAGLARIT"
     gene            5816..6376
                     /locus_tag="C2865_RS11805"
     CDS             5816..6376
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="C2865_RS11805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_198653191.1"
                     /transl_table=11
                     /translation="MTGPKEPGPAESGLAESGPEESGPEKFGPGELGSEESGPTESGLE
                     EPGLAELARRQEELVRALVAGARLPEGFDAARVGAAARALRNKRFGEVVRVWPSLLPYR
                     TEFCAWAEGRPTRGAERDGWEFARAHRSLLDDGAATDLAVLEARWRHADEPRRRRGPVV
                     RRAPGGLVLGLWGRVRVLRVRSG"
     misc_feature    complement(6422..6435)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.25"
                     /tool="antismash"
     gene            6425..7450
                     /locus_tag="C2865_RS11810"
     CDS             6425..7450
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017565081.1"
                     /locus_tag="C2865_RS11810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="6-phosphofructokinase"
                     /protein_id="WP_106398379.1"
                     /transl_table=11
                     /translation="MRIGVLTGGGDCPGLNAAIRAAVFRGVKEHGDEFTGFRDGWRGPL
                     ERDAFPLDAAAVGDILPLGGTILGSSRTNPLKEDDGVARIRRNLADLGLDALIAIGGED
                     TLGVANALHEAGVPVVGVPKTIDNDLSGTDQTFGFDTAVSIATEAADRLRTTAESHHRA
                     VVVEVMGRHAGWIALHTGLAAGADAVLVPEKPFDMAEVTSHVEARFRASRAAVLVVSEG
                     ARPAEGSLELQTGERDAFGHVRLGGVGERLAGELERRTGREARSVVLGHLQRGGTPTAF
                     DRVLATRFGLHAADAVRAGAYGTMVALRGTEITRVPLAEAVGTLKTVPPERYAEAEVFS
                     G"
     gene            7539..8750
                     /gene="thiI"
                     /locus_tag="C2865_RS11815"
     CDS             7539..8750
                     /EC_number="2.8.1.4"
                     /GO_function="GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006400 - tRNA modification [Evidence IEA]"
                     /GO_process="GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="thiI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017617062.1"
                     /locus_tag="C2865_RS11815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="WP_106398380.1"
                     /transl_table=11
                     /translation="MPLAPAAGEPCVLLKLGEIVLKGKNRHQFEQRLQSNIRKALASTG
                     VGVDLWQRSGVIIIRAGSGLAEDVEKVAKRLEDVMGIVIVHRTRRVPKDVESMTAAALE
                     AVAQHPMTARGGSFAVRPRRRDKRFPITSSQIGISVGSAINVKFGLPVDLKNPDITVSI
                     EVDRDEVFVYTDGAPGQGGLPVGSSGRGLVLMSGGIDSPVAAYRMMRRGLRVDYLHFSG
                     MPFTGPESIYKAYALVRELDKFQGDSRLYVVPFGKAQQQIKSAGEDRLAIVAQRRLMLK
                     TAEIVAERRRAGALITGDALGQVASQTLVNMTALDDAVDLPILRPLVGMDKIEIMDQAR
                     RNATLAISELPDEDCCSMLAPRRPETAARIADLRQIEKRLDAEELAEQLADSVQEHRPV
                     YGTD"
     gene            complement(8764..9732)
                     /locus_tag="C2865_RS11820"
     CDS             complement(8764..9732)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF039603.4"
                     /locus_tag="C2865_RS11820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF5954 family protein"
                     /protein_id="WP_157963667.1"
                     /transl_table=11
                     /translation="MPHEDEALEQGLHELTTTDDPVRALVAHDARRARREYPLTVLAGP
                     MFGVAEELGGDWRVITLGEATPQCSRDDLAGHLRLEARAAAEPEPYLAAARVLDWERYD
                     ELVVAGRRFRIIRVEQILRLNLSGEPEPPRPTDPDPRTAPAPRYETLGPSGRAGHPEAE
                     CAELAVRFAEAVEQPGAPNEQARSDARRAARSHPVPIPMLPAFSVAEYLHDGWRSSTAA
                     CPTPQRAREALAEYLQQVAPVVERVDEPTRTAYDRAAARLLAGRLDALEVCGRRFRIIR
                     VEKAARFGEDGPEPPRPSDFDPDPPVEVLALRLRAEGVIED"
     misc_feature    complement(9823..9840)
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 19.29"
                     /tool="antismash"
     misc_feature    9837..9850
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 12.58"
                     /tool="antismash"
     misc_feature    complement(9837..9852)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.91"
                     /tool="antismash"
     misc_feature    complement(9839..9852)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 14.96"
                     /tool="antismash"
ORIGIN
        1 cctgaccctc acccggggga ccgctcccgg tcaccgtggg tgctggagat ctcactgtgg
       61 atattcagca caagagcgtg tgttcccctg atttgccggg caggtgccgc taaagtccga
      121 aacttaggta aaggcggaga gggcagaccc gtgaagatcc tgatcaacct gggccgcggc
      181 gcgtccgtca cggtggcgcc gggcaggcgg cagtcgctgg tgttcgacaa ggaatggaac
      241 gacccggaca aggttcacaa ggacggcgcc cacgccgact ggctgcccaa ggagtcggcc
      301 taccacctgg ccaccctgac cgtcatcgcc gacggccagg ccgacggcga gaagctcaag
      361 ctcaccatca ccgagtacga gcgggacaag gacacccgga ccaaggacgc gctgggcgag
      421 gacaaggtcg gcaacggcac ccgcgtggag tacaccctct cggggctggt ctggatggac
      481 gagaagcggc gctaccgcgc cgaggtggag aacttcggca ccgtgccggt gaccgtcgag
      541 aaggcctacc tcaaagtggc gcggtgaagc ggttccctgc cgtgccgcgc gggctcagat
      601 gaccgcgccg tcgtcgtcgc cgtccctggg ctcgtcgccc atgcgcagca ccaggatgac
      661 gaagccgccg atgaaggcgg ccaccgccag gaacgcggcc cagcccggcg cggaccagcg
      721 gaagaccacc gtggccagga tgtagagcgg cccgccgacc aggcacagcc aggcgagcct
      781 gctggccggg tcgccggtcg gcagcggcgg gggaggcggc ggctcgtagt gcccctcctc
      841 gtccggctcg gggaagtccc cgtcctcctt gaccggaccc accgggccct cggagtagta
      901 gacgacacgg gcggtcggca gccggccgga gccgtcctgc aggtcgtcgc cggcgtcggc
      961 ggggccgagg ttctcctcgt cgggccacga cacgtcggtg ggcgtctcgc cgaaaccggc
     1021 cacgatctcc gcgaagatcg cgtcctcgtc gcgttcgggg ggcggcgcca cccggtcccg
     1081 cagcacgctg acgtgcagca gccgttcggc ctcgcccttg tgctcggcgt cgacgtgcac
     1141 atggtccagg acgtcgggac catcaccgcc ccccggcagc gcgtacgcgg cgatgcccgc
     1201 ctcgcgcagc acggtgagca gtcggtcggc gagttcgggg accaggtcga tcaggggcgc
     1261 gtaggcgtcc gctgacagcc cgttctcgcg gcggttcact tccctcaacc cctcaggact
     1321 ccggcgacca tcatgcgttg agcctacggt gcgaatcgtc ccacgggggg caagggggcg
     1381 cgatcgttcc gtctgaccga tcccgtgcga agatgtctcc cactttcatc gtcgggtacg
     1441 ggtcaaaggt cacggagaag aggcggtcac gatgttctgg tgggtggtca aggcgatcgt
     1501 ggggccgctc ctggtgctga tctggcggcc gaagaacgac ggcatggaga acgtgccgga
     1561 ggagggcccg gtcatcctgg ccagcaacca cctctcgttc gccgaccact tcttcggccc
     1621 ctggccgctg cgccgcagga tcgtcttcct cggcaagcgc gactacttca ccggcaaggg
     1681 cgtcaagggc gcgctcagcc ggatgttctt cagcggcgtc ggggtgatcc cgatcgaccg
     1741 caccggcggc aaggccagcg aggaggccct ggccaccggg ctgcgcgtgc tgcgcgaggg
     1801 caagctgctg ggcatctacc ccgagggcac ccgggtgccg gacaaccggc tgttcaaggg
     1861 caagaccggg gtggcgcggc tggccctgca gggcagggtg ccggtgatcc cgatggcgat
     1921 gatgcacacc ttcgagctga tgcccgcggg ctcgcggccg cgcgtccgcg gcctgcccgg
     1981 acggcccggc gtgcgcttcg gcgagccgat ggacttctcg cagtactacg gccgggagga
     2041 cgaccgcgag gtgctccgcc tgatcaccga caagatcatg atggcgatca gggagctgtc
     2101 cggccaggag tacgtcgacc gctacgccgc cgaggtcaag gccgagctcg ccggcaaggc
     2161 ggtcgtgcgc agggacgacg acgaggagtg aacgtcgagg ctgccctctg gcgggccctg
     2221 gcggtcttcc ggatcgtcgc ctgcctctac gcggcggtcc tgatcgcctc caaccaccag
     2281 tcctacctgc atccggcggg cggctggatc gtgctggccg tcatggtggc gtggacggcc
     2341 ttcaccgtcc gggcctacgc cgggggcggg gcggggccgg tcctggccgg tctcgacctc
     2401 gtcgtgtcca cggggtgcct gctggccacc gggctggtgg agaccgcgca gcacctggcc
     2461 gacgggcggc ccaacctgac ggccagctgg gtggcgatcc cggtcctggt gtgggcgctg
     2521 ctcggcggcg tgcgctgggg tgccgcggcg gcgctgctcg tgggcatccc cgacatgctg
     2581 ctgcgctggc tgtacggcgg ggagttccag cagaccaccc tcaacggcct ggtgctgctg
     2641 ctgatgggcg gggtggtggt cggctacctg ggcaggctgg cccgctacag ccaggagcgc
     2701 ctggcgcgcg cggtggcgct ggagaccgcg accagggagc gcgaacggct cgcccgcgac
     2761 ctgcacgact cggtgctgca ggtgctggcg ctggtgcggc ggcgggggac ggagctgggc
     2821 ggcgaggccg ccgagctggg caggctggcg ggggagcagg aggtggcgct gcgcgcgctg
     2881 atctcccgcc cggcgggcga ggaggagccc ggcggcgagc gcgacctgcg cgacctgctg
     2941 gaccgctacg cctcgtcctc ggtgcaggtc tccgccccgg cgacaccggt gcgcctgccc
     3001 ggccacgccg cggccgagct ggcggcggcg gtgggcgccg ccctcgacaa cgtctcccgc
     3061 cactgcggtc cggacgcgcg ggcgtgggtc ctggtggagg acgacgggga gacggtgacg
     3121 gtcagcgtcc gcgacgacgg gcccggcatc cccgaagggc ggatggaggc cgcggcgaag
     3181 ggcggccgca tgggcctggc ccggtcgatc aagggccgtg tccgcgacct gggcggcacc
     3241 gtcgagatct tctcctcgcc cggcgccggc accgagctgg agctgatcgt cccccgtccg
     3301 cgctgacgcg ggcggggtgc atgatgggcg gtgcgggacg agagggcccg cgcgtacggg
     3361 ccggagcccg gtgaggagag gacgatgacg gggatccggg tgatggtggt cgacgaccat
     3421 ccgatgtggc gcgaggccgt ggcgcgcgac ctggccgagg ccgggtacga ggtggtggcc
     3481 acggcgggcg aggggcgcaa ggcggtgcgg atcgccgcgg cggcccggcc gcaggtggcg
     3541 gtcgtggacc tgcagctgcc cgacatcagc ggggtgcagg tcgcccgctc gctggtggcc
     3601 gccgacccgt cggtgcgggt gctggtgctg tcggccagcg gggagcaggc cgacgtcctg
     3661 gaggcggtga aggcgggcgc caccggctac ctgctcaagt cagcggcccg cgaggagttc
     3721 ctggacgccg tggcgcgcac cgcgcagggc gacgcggtct tcacccccgg cctggccggg
     3781 ctggtgctgg gcgagtaccg caggctcgcc gccgcgccgg cccccgagcc cgacgccccg
     3841 cgcctgaccg accgcgagac cgagatcctg cgcctggtcg ccaagggcct gacctacaag
     3901 cagatcgccg agcgcctcgt cctctcccac cgcacggtcc agaaccacgt gcagaacacc
     3961 ctgggcaaac tccagctcca caaccgggtg gagctggtgc gttatgcgat cgagaagggt
     4021 ctcgacggcg aatagtcccc tccgccggaa cccggccgcc tcctcctgca tctatctggt
     4081 cgagaccggg cgatgcccgg gagaacgagg gagagtcaga tgacggactc gacgaccacg
     4141 tggggaatca acgggcctgc cttccttgga gtgttcgccc tggtggccgc ggcggcatgg
     4201 ctgctgggac tggcgatgcg ccgccggctg cgggcgggcc gggccccctc ccgcgaactg
     4261 cacccctacg aactggccta cctgcagggc ggcgcccggc tggtcgtcgc gtccgtcctg
     4321 gcggcgctgc ggatgtccgg gtcggtcacc gcgcggccgg gcggctccct gcgggccgag
     4381 ggcaccccgg tggcggcgcg ctccccgctg gaccaggcgg tcctgaaggc ggcgggccgg
     4441 gccgtgcgga ccggggagct ggccgaccac tccctcctgc ggccgcgcct ggacgagctg
     4501 cgcgacggcc tgcgcgccga ggggctgctg ctcggccccg aggaccggcg ggtcgtgcgg
     4561 atctccgcgc tgccgctgct gttcgtactg ctggtgggcc tggcgcggat ggcggcgggc
     4621 ctgcagaacg ggaagccggt cggctacctg atcgcgctgc tcgtggtcgc cgcggcagtg
     4681 ctggcgttca tgctgtacgt ccccgagaag acccgggccg tggacggcgt gctgctcgag
     4741 gcccggaccc gccacccgga gctgcagccg accgcctccc ccgactggcg gtcccacggc
     4801 cccgaggcgg cggcgctggg cgtggcgctg ttcggcgtcg gcgcgctggt ggccatggac
     4861 ccccagttcg ccgaggaggc cgagatccag cagcgcctcg gccagaccgg ctccggcggc
     4921 ggtgacggcg gaggcggtgg cgacggcggg gacggcggcg gcgggggagg cggctgcggc
     4981 ggttgcgggg gatgcggcgg atgacctctc ccggggtggg gctcggctgg cggcccccgc
     5041 tcgcgcggtt cgccgcggac ctgccgggac tggccggctc cggcttcgcc gaggtgatcg
     5101 ccgagaccct gcaccgcccc gatcccgcgc tgctggagct gcgcgaccgg ggcgcgcgga
     5161 tcgtcccgca cggcgtgcgg ctgtcactgg gcggcgccgc tccggtcgag cccgaacggg
     5221 tcgcccatct ggcgcactgc gccgagctgc tgggcgcgcc gctggtcagc gagcacgtgg
     5281 cgttcgtgcg tgccggaggc ctggaggcgg ggcacctgct gccggtgccc cgcagccgcg
     5341 cggcgctgga cgcgctgacc cgcaacatcc gcctcacccg cgcggagctg gacgtgccgc
     5401 tggccgtcga gccgatcgcg gcgctcttcg actggcccga cgacgagtac accgagggcg
     5461 acttcctcac cgagctgctc gaccggaccg gcgcgctgct cctgctggac gtggccaacg
     5521 tctacgccaa cgcgcgcaac agggggcggg acccggtgaa ggagctggag tccttcccgc
     5581 tcgaccgcgt cgcctactgc catgtggcgg gcggcggcga acacgacggg tactaccacg
     5641 acacccacac ccacgcgctg cccgaggagg tgctcgcgct ggtcggcgag gccgggcggc
     5701 gcaccaccgc cggcctggga tacctgctgg agcgcgacgg caactacccg cccgccgccg
     5761 cactgcgggc cgaactggac gcgatcgccg acgcggccgg gctggcgcgg atcacatgac
     5821 cggcccgaag gaacccggcc cggcggaatc cggtctggcg gagtccggcc cagaggagtc
     5881 cggtccggag aagttcggtc caggggagct cggctcggag gagtccggcc cgacggagtc
     5941 cggcttggag gagccagggc tggcggagct cgcccggcgg caggaggagc tggtccgcgc
     6001 cctggtggcc ggggcgcggc tgcccgaggg tttcgacgcg gcgcgggtcg gcgcggccgc
     6061 ccgcgcgctg cgcaacaaga ggttcgggga ggtcgtgcgc gtctggccgt cgctgctgcc
     6121 ctaccgcacg gagttctgcg cgtgggccga aggcaggccc acgaggggcg ccgagcggga
     6181 cggctgggag ttcgcccgcg cgcaccgctc gctgctggac gacggcgcgg cgaccgacct
     6241 ggccgtcctg gaggcgcgat ggcgccatgc agacgagccg cggaggcgcc gggggccggt
     6301 ggtgcgccgg gcgccgggag ggctcgtcct ggggctgtgg ggccgggtgc gggtactccg
     6361 agtacggagt ggttaggtcg gatgggagcc ctcgggcggg ctcacggacc gtgaaggggg
     6421 ctgcatgcgc atcggggtgc tcaccggggg aggcgactgc ccgggactca acgcggcgat
     6481 ccgggcggcg gtgttccggg gggtgaagga gcacggcgac gagttcacgg gctttcgcga
     6541 cggctggcgc ggccccctcg aacgcgacgc cttccctttg gacgcggcgg ccgtcgggga
     6601 catccttccg ctcggcggca cgatcctcgg ctcctcccgc accaacccgc tgaaggagga
     6661 cgacggggtc gcccgcatcc gccggaacct ggcggacctg ggactggacg ccctgatcgc
     6721 gatcggcggc gaggacaccc tgggcgtggc gaacgccctg cacgaggcgg gcgtgccggt
     6781 cgtgggcgtg cccaagacca tcgacaacga cctcagcggc accgaccaga ccttcggctt
     6841 cgacacggcc gtctccatcg ccaccgaggc cgccgaccgg ctgcgcacca ccgccgagag
     6901 ccaccacagg gcggtggtcg tggaggtgat gggcaggcac gccgggtgga tcgcgctgca
     6961 caccggcctg gccgcgggcg ccgacgccgt cctggtgccg gagaagccgt tcgacatggc
     7021 ggaggtcacc tcccacgtcg aggcgcggtt ccgggcgtcg cgcgcggcgg tcctcgtcgt
     7081 ctccgagggc gcccgccccg ccgagggctc gctggagctg cagacgggcg agcgcgacgc
     7141 cttcgggcac gtgcggctcg gcggcgtcgg ggagcggctc gccggggagc tggagcggcg
     7201 caccggccgc gaggcgcgct cggtcgtcct gggccacctc cagcgcggcg ggacgcccac
     7261 cgcgttcgac cgggtgctgg ccacccggtt cgggctgcac gccgccgacg ccgtccgcgc
     7321 cggggcctac ggcaccatgg tggcgctgcg cgggacggag atcacacggg tgcccctggc
     7381 ggaggcggtc ggcaccctca agaccgtgcc gcccgagcgg tacgccgagg ccgaggtctt
     7441 ctccggttga ggacgatctc cgataaccgg tcgaagtgct ctccggaatg ggcgacactg
     7501 aaagacggtt cccgcaacgt ctgacggagg tctgaatcat gcccctggcg cccgccgccg
     7561 gtgagccgtg tgtgctgctg aagctcggcg agatcgtcct gaagggcaag aaccgccatc
     7621 agttcgagca gcggctgcag agcaacatcc gcaaggcgct cgcctccacg ggggtcggcg
     7681 tcgacctctg gcagcgctcc ggtgtgatca tcatccgggc cggcagcggc ctggccgagg
     7741 acgtcgagaa ggtcgccaag cggctggagg acgtcatggg catcgtcatc gtgcaccgca
     7801 cgcggcgggt gcccaaggac gtcgagtcga tgaccgcggc cgcgctggag gcggtggccc
     7861 agcacccgat gacggcccgc ggcggcagct tcgcggtgcg cccgcgccgc cgcgacaagc
     7921 gcttcccgat cacctcctcg cagatcggga tctcggtcgg ctcggccatc aacgtcaagt
     7981 tcggtctgcc ggtcgacctg aagaaccccg acatcaccgt ctccatcgag gtcgaccgcg
     8041 acgaggtgtt cgtctacacc gacggcgcgc ccggccaggg cgggctgccg gtgggctcca
     8101 gcgggcgcgg tctggtgctg atgtccggtg gtatcgactc cccggtcgcc gcctaccgga
     8161 tgatgcgccg cggcctgcgg gtggactacc tgcacttctc cggcatgccc ttcaccggcc
     8221 ccgagtcgat ctacaaggcg tacgcgctgg tgcgcgagct cgacaagttc cagggcgact
     8281 cgcggctgta cgtggtgccg ttcggcaagg cgcagcagca gatcaagtcc gcgggcgagg
     8341 accggctggc gatcgtcgcc cagcggcggc tgatgctcaa gaccgccgag atcgtggccg
     8401 agcggcgccg cgccggcgcg ctcatcaccg gtgacgccct gggccaggtg gccagccaga
     8461 ccctggtcaa catgacggcg ctggacgacg cggtggacct gccgatcctg cgcccgctgg
     8521 tcggcatgga caagatcgag atcatggacc aggcgcgccg caacgccacc ctggccatct
     8581 ccgagctgcc cgacgaggac tgctgctcga tgctggcccc gcgccggccc gagaccgcgg
     8641 cgcgcatcgc cgacctgcgg cagatcgaga agcggctgga cgccgaggag ctggccgagc
     8701 agctggccga ctcggtgcag gagcaccgcc cggtctacgg cacggactga ccgccgcgcc
     8761 ggctcagtcc tcgatgaccc cttcggcgcg gagccgcagg gcgagcacct cgacgggcgg
     8821 gtcggggtcg aagtccgacg ggcggggcgg ctcggggccg tcctcgccga accgggcggc
     8881 cttctccacg cggatgatgc ggaagcgccg cccgcacacc tccagcgcgt ccagcctgcc
     8941 ggcgagcagg cgggccgccg cccggtcgta ggcggtgcgg gtgggctcgt ccacgcgttc
     9001 caccacgggg gcgacctgct ggaggtactc ggcgagggcc tcgcgggccc gctgcggggt
     9061 ggggcaggcg gcggtcgacg accgccaccc gtcgtgcagg tactcggcga cggagaaggc
     9121 cgggagcatg gggatgggca ccgggtgcga gcgcgccgcc cgccgggcgt ctgacctggc
     9181 ctgctcgttc ggcgccccgg gctgctcgac ggcctcggcg aagcgcacgg cgagctcggc
     9241 gcactcggcc tccgggtggc ccgcccggcc ggaggggccg agcgtctcgt accgcggggc
     9301 cggggcggtg cgcgggtcgg ggtcggtggg gcgcggcggt tcgggttcgc cgctgaggtt
     9361 cagccgcagg atctgctcca cgcggatgat ccggaaccgg cggcccgcca cgacgagctc
     9421 gtcgtagcgc tcccagtcca gcacgcgggc cgcggccagg tagggctcgg gctcggcggc
     9481 cgcgcgcgcc tccagccgca ggtggccggc caggtcgtcg cgggagcact ggggggtggc
     9541 ctcccccagg gtgatcacgc gccagtcccc gccgagttcc tcggcgaccc cgaacatggg
     9601 accggccagg acggtcagcg ggtactcccg ccgggcccgc cgggcgtcgt gcgcgacgag
     9661 cgcccgcacc gggtcatcgg tggtggtcag ctcgtgtagc ccttgttcca gagcctcgtc
     9721 ctcatggggc atgccccaca tggtcccctg cccctaccgt actttcgccg attcgcgccg
     9781 gatcgccgat acggttccgc tttccggacg gcctcggagg aaaatcctca acccgggata
     9841 aagatcctta tactctgacg gccgggcgcg cggagatccg cacgcccggc cgccgggcca
     9901 cctcaggccg tggccgcctc aggccgggac ggccgcggcg gggacggtga gcgagcggcc
     9961 caccaccggg ggcagcgcgc gcaccagctc ggccagctcg gcgaggtcct cccggaccag
    10021 cgcctggggg ccgtcgcaca gcgcggtctc ggggtgcggg tgcacgtcga tgatgacgcc
    10081 gtcggcgccg atcgcgatgg ccgcgcgggt gagcgggagc accaggtcgc ggtggccgcc
    10141 ggagtgggac gggtccacga tcaccggcag gtgcgacaac cgctgggcca ccgggaccgc
    10201 ggagatgtcc agggtgttgc gggtggcctt ctcgaaggtg cggatgccgc gctcgcacag
    10261 gacgacgtcg aggttgccgc gctgggcgac gtactcggcc gacatcagcc actcctcgat
    10321 ggtcccgctc atgccgcgct tgatcatgac gggcttgccg gcctcgccga ccgcctgcag
    10381 cagcgcgaag ttctgggtgt tgcgggtgcc gatctgcagc atgtcggcgt accgggcgac
    10441 cagttccacg tcgcgcgcgt ccaccacctc ggtcacgacc ggcatgccgg tctcggcccg
    10501 cacgtccgcc aggatgcgca gcccctcctc gcccaggccc tggaaggcgt acggggaggt
    10561 gcggggcttg aaggcgccgc cgcgcagcag ggtggcgccg gccgactgcg ccatgcgggc
    10621 ggcctcgagg gtctgctcgg gggtctccac cgcgcagggg ccggcgatca gggtcatggc
    10681 gccggggccg atcggcacgc cgccgacgtg cacggtcgag cgctccgcgt ggtgctcgcg
    10741 gctcaccagc ttgtaggggg cgctgatccg caccacctcg acgacgccgc gctggccgcc
    10801 gaggttgagc gcctcgaag
//
