LOCUS       NZ_JZOJ01000049         6012 bp    DNA     linear   CON 27-MAY-2024
DEFINITION  Escherichia coli strain HT2012031 NODE_99_length_5938_cov_52.979622,
            whole genome shotgun sequence.
ACCESSION   NZ_JZOJ01000049
VERSION     NZ_JZOJ01000049.1
KEYWORDS    WGS; RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Lu,S., Jin,D., Wu,S., Yang,J., Lan,R., Bai,X., Liu,S., Meng,Q.,
            Yuan,X., Zhou,J., Pu,J., Chen,Q., Dai,H., Hu,Y., Xiong,Y., Ye,C. and
            Xu,J.
  TITLE     Insights into the evolution of pathogenicity of Escherichia coli
            from genomic analysis of intestinal E. coli of Marmota himalayana in
            Qinghai-Tibet plateau of China
  JOURNAL   Emerg Microbes Infect 5 (12), e122 (2016)
   PUBMED   27924811
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6012)
  AUTHORS   Xu,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2015) New pathogen brach, National institute for
            communicable disease control and prevention, P.O. Box 5 Changping,
            Beijing, Beijing 102206, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Velvet v. April 2014
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 40.0x
            Sequencing Technology             :: Illumina GAIIx
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002972405.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 01:49:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,368
            CDSs (total)                      :: 5,292
            Genes (coding)                    :: 4,983
            CDSs (with protein)               :: 4,983
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 78 of 309
            Pseudo Genes (incomplete)         :: 257 of 309
            Pseudo Genes (internal stop)      :: 43 of 309
            Pseudo Genes (multiple problems)  :: 62 of 309
            Pseudo Genes (short protein)      :: 2 of 309
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:14:29
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 6012
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JZOJ01000049.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6012
                     /collection_date="2012"
                     /country="China"
                     /db_xref="taxon:562"
                     /isolation_source="animal"
                     /mol_type="genomic DNA"
                     /organism="Escherichia coli"
                     /strain="HT2012031"
                     /submitter_seqid="NODE_99_length_5938_cov_52.979622"
     region          1..6012
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lassopeptide"
                     /region_number="1"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /tool="antismash"
     cand_cluster    1..6012
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..6012
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[615:3304]"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      616..3304
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     gene            300..395
                     /gene="dqlB"
                     /locus_tag="VE62_RS29665"
     CDS             300..395
                     /codon_start=1
                     /gene="dqlB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609144.1"
                     /locus_tag="VE62_RS29665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="WP_000609143.1"
                     /transl_table=11
                     /translation="MINLLIIILRDVVAVANALIAVLELIRQFIV"
     gene            complement(616..792)
                     /gene="mcjA"
                     /locus_tag="VE62_RS06215"
     CDS             complement(616..792)
                     /codon_start=1
                     /gene="mcjA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: micJ25"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513516.1"
                     /locus_tag="VE62_RS06215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lasso peptide microcin J25"
                     /protein_id="WP_096488204.1"
                     /sec_met_domain="micJ25 (E-value: 5.5e-26, bitscore: 79.1,
                     seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIKHIHFDKLSSGKKNNVPHPAKGVIQIKKSASQLTKGGAGHVPE
                     YFVGIGTPISFYG"
     CDS_motif       complement(682..792)
                     /prepeptide="leader"
                     /note="peptide class: lassopeptide"
                     /note="predicted sequence:
                     MIKHIHFDKLSSGKKNNVPHPAKGVIQIKKSASQLTK"
                     /locus_tag="VE62_RS06215_lassopeptide"
                     /aSTool="lassopeptides"
                     /tool="antismash"
     CDS_motif       complement(616..681)
                     /prepeptide="core"
                     /locus_tag="VE62_RS06215_lassopeptide"
                     /peptide="lassopeptide"
                     /predicted_class="Class II"
                     /score="5.40"
                     /molecular_weight="2107.3"
                     /monoisotopic_mass="2106.0"
                     /core_sequence="GGAGHVPEYFVGIGTPISFYG"
                     /aSTool="lassopeptides"
                     /tool="antismash"
                     /leader_sequence="MIKHIHFDKLSSGKKNNVPHPAKGVIQIKKSASQLTK"
                     /leader_location="[681:792](-)"
     gene            1123..1395
                     /locus_tag="VE62_RS28925"
     CDS             1123..1395
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lasso_Fused_RRE"
                     /gene_functions="biosynthetic-additional (MITE)
                     MITE0000056: Hydrolysis (97% identity)"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="VE62_RS28925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_244597508.1"
                     /sec_met_domain="Lasso_Fused_RRE (E-value: 1.8e-15,
                     bitscore: 45.8, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYKMIRYCLTSYREDLVILDIINDSFSIVPDAGSLLKEKDKLLKE
                     YPQLSYFFDSEYHIGSVSRNSDTSFLEERWFLPEPDKTLYNVFSI"
     aSDomain        1132..1326
                     /aSDomain="Lasso_Fused_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing fused peptidase in a lasso
                     peptide cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_VE62_RS28925_Lasso_Fused_RRE.1"
                     /evalue="7.30E-15"
                     /identifier="RREFam003.1"
                     /label="VE62_RS28925"
                     /locus_tag="VE62_RS28925"
                     /protein_end="68"
                     /protein_start="3"
                     /score="45.8"
                     /tool="antismash"
                     /translation="MIRYCLTSYREDLVILDIINDSFSIVPDAGSLLKEKDKLLKEYPQ
                     LSYFFDSEYHIGSVSRNSDT"
     gene            1455..1760
                     /locus_tag="VE62_RS28930"
     CDS             1455..1760
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_functions="biosynthetic-additional (MITE)
                     MITE0000056: Hydrolysis (99% identity)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF033537.1"
                     /locus_tag="VE62_RS28930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="WP_244595832.1"
                     /sec_met_domain="PF13471 (E-value: 1.4e-24, bitscore: 75.4,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAWIFISNKKENRLYSLNEEHLIRKEISNLSIIFHLNIFKSDCLT
                     YSYALKRILNSRNIDAHLVIGVRTQPFYSHSWVEVGGKVINDAPNMRDKLSVIAEI"
     misc_feature    1480..1500
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 26.44"
                     /tool="antismash"
     gene            1763..3304
                     /locus_tag="VE62_RS06225"
     CDS             1763..3304
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: mcjC"
                     /gene_functions="biosynthetic-additional (MITE)
                     MITE0000055: Macrolactam formation (99% identity)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_097345836.1"
                     /locus_tag="VE62_RS06225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lasso peptide isopeptide bond-forming cyclase"
                     /protein_id="WP_105456018.1"
                     /sec_met_domain="mcjC (E-value: 0.0, bitscore: 1214.2,
                     seeds: 3, tool: rule-based-clusters)"
                     /sec_met_domain="Asn_synthase (E-value: 8.7e-09, bitscore:
                     24.3, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEIFNVKLNDTSIRIIFCKTLSAFRTENTIVMLKGKAVSNGKPVS
                     TEEIARVVEEKGVAEVIENLDGVFCILIHHFNDLLIGRSIQSGPALFYCKKNMDIFVSD
                     KISDIKFLNPDMTFSLNIKMAEHYLSGNRIATQESLITGIYKVNNGEFIKFNNQLKPVL
                     LRDEFSITKKNNSTIDSIIDNIEMMRDNRKIALLFSGGLDSALIFHTLKESGNKFCAYH
                     FFSDESDDSEKYFAKEYCSKYGVDFISVNKNINFNEKLYFNLNPNSPDEIPLIFEQTDE
                     EGEGQPPIDDDLLYLCGHGGDHIFGQNPSELFGIDAYRSHGLMFMHKKIVEFSNLKGKR
                     YKDIIFSNISAFINTSNGCSPAKQEHVSDMKLASAQFFATDYTGKINKLTPFLHKNIIQ
                     HYAGLPVFSLFNQHFDRYPVRYEAFQRFGSDIFWKKTKRSSSQLIFRILSGKKDELVNT
                     IKQSGLIEILGINHIELESILYENTTTRLTMELPYILNLYRLAKFIQLQSIDYKG"
     misc_feature    1798..1813
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 23.32"
                     /tool="antismash"
     gene            3381..5042
                     /locus_tag="VE62_RS06230"
     CDS             3381..5042
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /locus_tag="VE62_RS06230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_249922359.1"
                     /transl_table=11
                     /translation="MLFITSLSSIIISISPLILAKITDLLSGSLSNFSYEYLVLLACLY
                     MFCVISNKASVFLFMILQSSLRINMQKKMSLKYLRELYNENITNLSKNNAGYTTQSLNQ
                     ASNDIYILVRNVSQNILSPVIQLISTIVVVLSTKDWFSAGVFFLYILVFVIFNTRLTGS
                     LASLRKHSMDITLNSYSLLSDTVDNMIAAKKNNALRLISERYEDALTQENNAQKKYWLL
                     SSKVLLLNSLLAVILFGSVFIYNILGVLNGVVSIGHFIMITSYIILLSTPVENIGALLS
                     EIRQSMSSLAGFIQRHAENKAASPSIPFLNMERKVSLSIRELSFSYSDDKKILNSVSLD
                     LFTGKMYSLTGPSGSGKSTLVKIISGYYKNYSGDIYLNDISLCDISDEDLNEAIYYLTQ
                     DDYIFMDTLRFNLRLANYDASENEMFKVLKLANLSVVNNQPVNLDTHLINRGNNYSGGQ
                     KQRISLARLFLRKPAIIIIDEATSALDYINESEILSSIRTHFPDALIINISHRINLLEC
                     SDCVYVLDEGNIVASGHFRDLMVSNEYISGLASVTE"
     CDS             complement(5142..>6010)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060658.1"
                     /locus_tag="VE62_RS06235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Tn3 family transposase"
                     /protein_id="WP_157942341.1"
                     /transl_table=11
                     /translation="GRGKGLVAYTLLCNHIPINGYLIGTNDYEGHHVFDIWYRNTSVVK
                     PVAITGDMHSVNKANFAILHWFGLRFEPHFTDLNRQLQELYSTREASVYKKCLIQPAGR
                     IDLELISREKSNLDRIVATLGLKEMTQGTLIRKLCTYTTTNPTRQAVFEYDRLIRSIYT
                     LKYLRDPQMERNIRRSQNRIESYHQLRAAVAKVGGKKELTGKNDIETEISNQCGRLISN
                     AIVYYNSAILSRLLRRLETEGNEKSIEALTRISPVAWQHILLNGHYTFQNNNELIDLDT
                     LVAGLKLG"
     gene            complement(5142..>6012)
                     /locus_tag="VE62_RS06235"
ORIGIN
        1 aagcgattag tacgaagtgg cctgtatcgt tcaaggggat ggtcaatttc gttccacagg
       61 taatctccag tgagtacgac aaaccttaaa attgaagttt ccgtacagtt tctcattccg
      121 acagggtttg tcacgcaaaa aatcggagat ttatggattc aattttcttc aactgcagga
      181 cctgacggaa aacaagtgat ttccgtcagg gagaaatcgc ttagcgggtg ttatcgcaga
      241 aattcccacg gagccccttt atgggggtca gttcagtgag gttgggctaa gagaaacgaa
      301 tgattaacct gttaatcatc attctcaggg atgtggtcgc cgttgcaaac gcgctgattg
      361 cagttctgga actgattcgc cagttcatcg tttgatgaca gcggaaacgt aactaaggac
      421 aggaagttcc aaatagctcc catccagcat atgtaatttt caaaggggca gttgttaact
      481 ctgtaataac accatgtaaa gtattcctgc cccctccatg atcctccata gtatttcttg
      541 agttaatttt caaaaagctt ctgatatgtg atttattgtt gatagacaaa atgacagatt
      601 tcttctttca gaagatcagc catagaaaga tataggtgta ccgatcccca caaaatactc
      661 aggcacatgt cctgcaccac cttttgtgag ttgcgatgct gattttttta tttgtataac
      721 tccctttgca gggtgtggaa cattattttt tttgccggaa gacaatttat caaaatgaat
      781 atgcttaatc atttttacgt tccttatatg atgaaaataa tataatgatt tttacagagg
      841 aacctcactt ccttctgtta gaattttatt agcataaaac gcaattgaga tcaagaatca
      901 ttacgtttta ttcatttatc aataaaatca tttagatatc aacatcctta aagtaatcaa
      961 tctgcaaaag tggttaaatt tagtacaata ttttgtatat ttatacattt ttctaattat
     1021 ttcagaatat ttagccatca attaagaaaa aaatttagct tgtagataaa ttcagaagtt
     1081 ttattattcc aattgagtgt aaaggcgtaa ctacaggagg gaatgtacaa aatgatccgt
     1141 tactgcttaa ccagttatag agaggatctt gttattctgg atataattaa tgatagtttc
     1201 agcatagtgc ctgacgcagg tagcttgcta aaagaaaaag ataaattgct taaagaatac
     1261 ccacaactat cttacttttt tgacagtgaa tatcatattg gaagtgtttc tcgtaatagt
     1321 gacacttctt ttcttgaaga acgctggttt ctaccagaac ctgacaaaac attatataat
     1381 gtgttctcta tttaaacgat ttatattatt actcaaagtc ttttactata gctggaatat
     1441 tgaaaaaaaa agggatggca tggattttca taagtaataa aaaagagaat aggctatact
     1501 ctttgaatga agagcatctt atccggaaag aaattagtaa tctttccatt atctttcatc
     1561 ttaatatttt taaatctgac tgtcttacct attcatacgc actaaaaaga attcttaatt
     1621 ccagaaatat tgatgctcat cttgttattg gtgtaaggac acagcctttt tatagtcact
     1681 cttgggtgga ggttggggga aaagttatca atgatgctcc caatatgcgg gataaattat
     1741 ctgttattgc agagatatag ttatggaaat atttaatgtc aagttaaatg atacttcaat
     1801 tagaattatt ttctgtaaaa cgctttctgc cttccggaca gaaaatacca tcgtcatgct
     1861 caaaggaaaa gcagtttcaa atggcaagcc tgtatccaca gaggagattg ccagagtagt
     1921 ggaagaaaaa ggtgttgcag aagtaataga aaatttagat ggtgttttct gtatcctaat
     1981 tcatcatttt aatgatctcc ttatagggag aagcattcaa tcaggccccg ctctatttta
     2041 ttgtaaaaag aatatggata tttttgtttc ggataaaatt tctgatatca aatttttgaa
     2101 tccagatatg acattcagtc taaatataaa aatggcagaa cattatctgt caggaaatcg
     2161 aatagcaacc caggaatcac taatcactgg catttacaaa gtaaataatg gtgagtttat
     2221 aaaatttaat aatcagttga aacctgtgct acttcgtgat gagtttagta ttaccaaaaa
     2281 gaacaattca actatcgaca gtatcattga taatattgag atgatgcggg acaatagaaa
     2341 aatagcccta ttattctccg gaggattgga ttctgcatta atttttcaca cacttaaaga
     2401 atcaggtaac aaattctgcg cttatcattt tttttctgat gaatctgatg acagtgaaaa
     2461 gtattttgct aaggaatact gttcaaaata tggagttgat tttatatctg ttaataaaaa
     2521 tatcaacttt aatgaaaaac tttatttcaa tttaaatcct aatagtccgg acgaaatccc
     2581 tttgatattt gaacagacag atgaagaagg tgaaggtcag ccccccatag acgatgattt
     2641 attatatcta tgtggtcacg gtggagatca tattttcgga caaaatcctt cagaactttt
     2701 tggcattgat gcatatcgaa gtcatggctt gatgtttatg cataaaaaaa tagtagaatt
     2761 ttccaatctc aagggaaaga gatataaaga tatcatattt tcaaatattt ccgcattcat
     2821 taatacatcc aacggatgtt ctccagcaaa gcaagagcac gtatcagata tgaaacttgc
     2881 ctctgctcag ttttttgcaa ctgattatac aggaaaaatt aataaactaa ctccattcct
     2941 acataaaaat attatccagc attatgctgg cttaccagtt tttagtctat ttaaccagca
     3001 ctttgatcgt tatcccgttc gttatgaagc gtttcaaaga tttggttcag atattttctg
     3061 gaaaaaaacc aaacggtcat cttcacagct aatattcaga attctatccg gtaaaaagga
     3121 tgaactagtg aatacaataa aacagtcagg attaattgaa atattaggca ttaaccatat
     3181 tgaattagaa agcattttgt atgaaaatac gaccacacgt ctgacaatgg aactaccata
     3241 tatacttaac ttataccgtc tggcaaaatt cattcaactt caatccattg attataaagg
     3301 ttaattatgg aaagaaaaca gaaaatatca ttatttaatt atatttactc attaatggat
     3361 acaagaggtt attcttttcc atgttattca ttacatcatt atcatcaata atcatatcta
     3421 tttcaccatt gatccttgca aagattacag atttactgtc tggctcattg tcaaatttta
     3481 gttatgaata tctggtttta cttgcctgtt tatacatgtt ttgcgttata tctaataaag
     3541 caagtgtttt tttatttatg atactgcaaa gtagtctacg tattaacatg cagaaaaaaa
     3601 tgtcgctaaa atatttgaga gaattgtata acgaaaatat aaccaatttg agtaaaaata
     3661 atgctggata tacaacacaa agtcttaacc aggcttcaaa tgatatttat attcttgtga
     3721 gaaatgtttc ccagaacatc ctgtcacctg ttatacaact tatttctact attgttgttg
     3781 ttttatctac gaaggactgg ttttctgccg gtgtgttttt tctctatatt ctggtatttg
     3841 taatttttaa taccagacta actggcagtt tagcgtcact aagaaaacac agcatggata
     3901 tcactcttaa ctcttatagt ctgttatctg atactgttga taacatgata gcagctaaaa
     3961 agaataatgc attaagactt atttctgaac gttatgaaga tgctctcact caggaaaaca
     4021 atgctcagaa aaaatactgg ttactcagtt ctaaagttct tttattgaac tctttacttg
     4081 ctgtaatatt atttggttct gtattcatat ataatatttt aggtgtactg aatggtgtag
     4141 ttagtatcgg ccacttcatt atgattacat catatatcat tcttctttca acgccagtgg
     4201 aaaatatagg ggcattgcta agtgagatca ggcagtcaat gtctagcctg gcaggtttta
     4261 ttcaacgtca tgccgagaat aaagccgcct ctccttcaat accttttctc aacatggaga
     4321 gaaaagtaag cctgtccata agagaacttt catttagcta tagtgatgat aaaaaaatac
     4381 ttaattcagt cagtcttgac ctttttaccg gaaaaatgta ttcattaacc ggacccagtg
     4441 gttcaggaaa atccaccctt gtaaaaataa tatcaggtta ctataaaaat tactctggag
     4501 acatttatct gaatgatata tccttatgtg atatcagtga cgaggatttg aatgaggcta
     4561 tttactacct tacacaagat gattatattt ttatggatac actacggttt aacctccgac
     4621 tcgcaaatta cgacgcgtca gaaaatgaaa tgtttaaagt tcttaaactg gcaaatcttt
     4681 ctgtcgttaa caatcaacca gtgaatctgg atacgcacct tataaacaga ggcaataact
     4741 attcaggagg gcaaaaacaa cgaatttcgt tagcgcggct gtttttgaga aaacctgcaa
     4801 taattattat tgatgaagcc acatcggctc tggattatat taatgaatca gaaattttat
     4861 catcaataag aactcatttt cctgatgcgc taattataaa tattagtcac cgaataaatc
     4921 ttctggagtg ttccgattgt gtttatgtat tggatgaagg aaatattgtt gcttctggcc
     4981 atttcaggga tttgatggtc agcaatgaat acatatcggg actggcttct gttactgaat
     5041 aaaatatcag actcctgctc tgtattcaga aagggagtcg cttggaaaac ggaatttttc
     5101 ccacgttaag gggttctacg ccggaaccgc cgaaatttcc gttatcccag ttttaaaccg
     5161 gctaccaatg tatccaggtc aataagttca ttattgttct ggaaggtata atgtccgttc
     5221 agcaaaatgt gttgccaggc tactggtgat atcctggtca gagcctcaat ggacttttca
     5281 ttaccttctg tttccagtcg cctcagcaaa cgtgacagga tcgcagagtt gtaataaacg
     5341 atcgcgttgg agatcaggcg accgcactga ttactgatct ctgtttctat atcgtttttt
     5401 ccggttaact ctttctttcc accaactttg gccactgctg cacgtaactg gtgataagac
     5461 tctatccggt tctgggaacg gcgaatattg cgttccattt gcggatcgcg cagatacttc
     5521 agcgtataaa tgctgcgaat cagacggtca tattcaaata ccgcctgcct tgtaggatta
     5581 gttgttgtat atgtacataa cttacggatc agcgttccct gcgtcatttc cttcagaccc
     5641 agcgtggcaa cgatgcgatc gagattgctt ttctcccggg atataagctc caggtctatc
     5701 cgaccggctg gctgaatcag gcatttttta tagaccgacg cttccctggt gctgtacaac
     5761 tcctgtaatt gcctgttcag gtcagtaaaa tgaggctcaa aacgaaggcc aaaccagtgc
     5821 agtatcgcaa aatttgcctt gttaacactg tgcatatccc cggtgatggc caccggtttc
     5881 actacagagg tattgcggta ccagatatca aaaacgtgat gtccttcgta atcattcgtg
     5941 ccaatcaggt atccgttgat cgggatatgg ttacacaaca gagtgtaggc caccaggcct
     6001 ttaccacgac ca
//
