LOCUS       NZ_JZOJ01000007         8874 bp    DNA     linear   CON 27-MAY-2024
DEFINITION  Escherichia coli strain HT2012031
            NODE_10_length_11341_cov_62.100254, whole genome shotgun sequence.
ACCESSION   NZ_JZOJ01000007
VERSION     NZ_JZOJ01000007.1
KEYWORDS    WGS; RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11415)
  AUTHORS   Lu,S., Jin,D., Wu,S., Yang,J., Lan,R., Bai,X., Liu,S., Meng,Q.,
            Yuan,X., Zhou,J., Pu,J., Chen,Q., Dai,H., Hu,Y., Xiong,Y., Ye,C. and
            Xu,J.
  TITLE     Insights into the evolution of pathogenicity of Escherichia coli
            from genomic analysis of intestinal E. coli of Marmota himalayana in
            Qinghai-Tibet plateau of China
  JOURNAL   Emerg Microbes Infect 5 (12), e122 (2016)
   PUBMED   27924811
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11415)
  AUTHORS   Xu,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2015) New pathogen brach, National institute for
            communicable disease control and prevention, P.O. Box 5 Changping,
            Beijing, Beijing 102206, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Velvet v. April 2014
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 40.0x
            Sequencing Technology             :: Illumina GAIIx
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002972405.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 01:49:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,368
            CDSs (total)                      :: 5,292
            Genes (coding)                    :: 4,983
            CDSs (with protein)               :: 4,983
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 78 of 309
            Pseudo Genes (incomplete)         :: 257 of 309
            Pseudo Genes (internal stop)      :: 43 of 309
            Pseudo Genes (multiple problems)  :: 62 of 309
            Pseudo Genes (short protein)      :: 2 of 309
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:14:29
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2541
            Orig. end                         :: 11415
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JZOJ01000007.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..8874
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..8874
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..8874
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:6536](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6536
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            210..1910
                     /gene="ltrA"
                     /locus_tag="VE62_RS00330"
     CDS             210..1910
                     /EC_number="2.7.7.49"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ltrA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125551.1"
                     /locus_tag="VE62_RS00330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_105456131.1"
                     /transl_table=11
                     /translation="MTAKNINAGAPTDIVTHWHNINWARCRREVRRLQARIVKATQEEK
                     AGKVKALQWLLTHSFSARAMAVKRVTDNRGKRTPGVDGKTWSKPGSKMKAIYTLKRRGY
                     KPLPLRRIYIPKSNGKKRPLGIPTMKDRAMQALYLMALEPVAETTADPNSFGFRPCRST
                     ADAIEQCFTTLHRADRAQWILEADIRSCFDEISHEWLIANIPTDTAILKRWLKAGYIDL
                     GKLYPTSAGTPQGGIISPTLANMVLDGLQPLLKKTFYRSGLNPEKINIIRYADDFVITG
                     ISHDTLSEKVLPLLENFLADRGLTLSPEKTRITHISDGFDFLGMNIRKYNGKLLIKPSP
                     QKVKEFLKRVRQMIKDNPTVSQSTLIWHLNPILKGWARYYRHVVSKKIFHKVSHEIWKA
                     LWRWACRRHPKKGTGWIRRKYFRSVNFRHWVFGTETGKILPSGKRELLALYEISDMPIK
                     RHRQIKSTANPFDPSYESYFEARLKVTMLESIRGYSRLRNLWLRQGGLCPVCRLPVNST
                     TGWQLRHIVPKSEGGLDNIGNLAMLHPACHHTAKHQGISVMKPALMWSY"
     gene            complement(<1992..2063)
                     /locus_tag="VE62_RS28285"
                     /pseudo=""
     CDS             complement(<1992..2063)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /locus_tag="VE62_RS28285"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="adenine-specific DNA-methyltransferase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MSRFIQGDCVRVMATFPGNAVDFM"
     gene            complement(2140..2367)
                     /locus_tag="VE62_RS00335"
     CDS             complement(2140..2367)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589601.1"
                     /locus_tag="VE62_RS00335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_170942586.1"
                     /transl_table=11
                     /translation="MTRRGRNAACGQCPASGRARDGRFYRSRLALVTVYASPSPFSDER
                     PSSRFRGISSPSKRRRLRYSTVGLTRYRTR"
     gene            2268..2474
                     /locus_tag="VE62_RS29750"
     CDS             2268..2474
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311063.1"
                     /locus_tag="VE62_RS29750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_306425709.1"
                     /transl_table=11
                     /translation="MSPTPAGSGKSARPVPGRTRDTDHRQRSCRAASAGAVKRGARRHL
                     LLPAKQNPKTTQTKPNKTPKQDK"
     gene            2579..3541
                     /gene="parM"
                     /gene_synonym="stbA"
                     /locus_tag="VE62_RS00340"
     CDS             2579..3541
                     /codon_start=1
                     /gene="parM"
                     /gene_synonym="stbA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /locus_tag="VE62_RS00340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="plasmid segregation protein ParM"
                     /protein_id="WP_000959883.1"
                     /transl_table=11
                     /translation="MLVFIDDGSTNIKLQWQESDGTIKQHISPNSFKREWAVSFGDKKV
                     FNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSGLPVSEVDIVCTLPL
                     TEYYDRNNQPNTENIERKKANFRKKITLNGGDTFTIKDVKVMPESIPAGYEVLQELDEL
                     DSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGSSYLA
                     DDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHVMVIGG
                     GAELICDAVKKHSQIRDERFFKTNNSQYDLVNGMYLIGN"
     gene            3544..3894
                     /locus_tag="VE62_RS00345"
     CDS             3544..3894
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /locus_tag="VE62_RS00345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="WP_000361384.1"
                     /transl_table=11
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCDTLDSIPQGERSRLNRAALTAG
                     LALYRQDPRAPFLLCELLTKETTFSDIVNILRSLFPKEMADFNSSTITQPSSQQEQRSD
                     EETKKNAMKLIN"
     gene            complement(4045..4560)
                     /locus_tag="VE62_RS00350"
     CDS             complement(4045..4560)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027529.1"
                     /locus_tag="VE62_RS00350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_105456130.1"
                     /transl_table=11
                     /translation="MNISVEDLFPESFQRIKEEEEEANRPDPNWFAHLPPEKINAYMSR
                     YPFKSFDDIPLDNSGLQYPSLQELEFYFPPAALAANCHQLPERMRQKPVVTMINGLTLL
                     RSLGVQAFNIDPRRWHKIKTYLSQGTIEYPQMSEDGKVIIDGRHRILLIMQIYKVTDVP
                     VFFLKGIS"
     misc_feature    4621..4636
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 20.61"
                     /tool="antismash"
     misc_feature    complement(4848..4864)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 21.16"
                     /tool="antismash"
     misc_feature    4852..4861
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.57"
                     /tool="antismash"
     misc_feature    4924..4942
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: medium, score: 25.7"
                     /tool="antismash"
     misc_feature    complement(4940..4958)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: medium, score: 24.97"
                     /tool="antismash"
     gene            5001..6536
                     /gene="cba"
                     /locus_tag="VE62_RS00360"
     CDS             5001..6536
                     /codon_start=1
                     /gene="cba"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Cloacin"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282376.1"
                     /locus_tag="VE62_RS00360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="pore-forming bacteriocin colicin B"
                     /protein_id="WP_105456129.1"
                     /sec_met_domain="Cloacin (E-value: 3.6e-95, bitscore:
                     307.6, seeds: 4, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDNEGSVPTEGIDYGDTMVVWPSTGRIPGGDVKPGGSSGLAPSM
                     PSGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAART
                     VLENGLRGGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPASQLPAAGN
                     NVPVKYRLTDLMQNGTQYMAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPVSPNL
                     PDLVLSVGQMNTPVRSNPEIQEDGVISETGNYVEAGYTMSSNNHDVIVRFPEGSGVSPL
                     YISAVEILDSNSLSQRQEAENNAKDDFRVKKEQENDEKTVLTKTSEVIISVGDKVGEYL
                     GDKYKALSREIAENINNFQGKTIRSYDDAMSSINKLMANPSLKINATDKEAIVNAWKAF
                     NAEDMGNKFAALGKTFKAADYAIKANNIREKSIEGYQTGNWGPLMLEVESWVISGMASA
                     VALSLFSLTLGSALIAFGLSSTVVGFVGVVIAGAIGAFIDDKFVDELNHKIIK"
     gene            complement(6554..7081)
                     /gene="cbi"
                     /locus_tag="VE62_RS00365"
     CDS             complement(6554..7081)
                     /codon_start=1
                     /gene="cbi"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203268.1"
                     /locus_tag="VE62_RS00365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="colicin B immunity protein"
                     /protein_id="WP_016233052.1"
                     /transl_table=11
                     /translation="MTSNKDKNKKANEILYAFSIIGIIPLMAILILRINNPYSQVLYYL
                     YNKVAFLPSITSLHDPVMTTLMSNYNKTAPVMGILVFLCTYKTREIIKPVTRKLVVQSC
                     FWGPVFYAILIYITLFYNLELTTAGGFFKLLSHNVITLFILYCSIYFTVLTMTYAILLM
                     PLLVIKYFKGRQ"
     gene            7325..8140
                     /gene="cma"
                     /locus_tag="VE62_RS00375"
     CDS             7325..8140
                     /codon_start=1
                     /gene="cma"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449473.1"
                     /locus_tag="VE62_RS00375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lipid II-degrading bacteriocin colicin M"
                     /protein_id="WP_000449473.1"
                     /transl_table=11
                     /translation="METLTVHAPSPSTNLPSYGNGAFSLSAPHVPGAGPLLVQVVYSFF
                     QSPNMCLQALTQLEDYIKKHGASNPLTLQIISTNIGYFCNADRNLVLHPGISVYDAYHF
                     AKPAPSQYDYRSMNMKQMSGNVTTPIVALAHYLWGNGAERSVNIANIGLKISPMKINQI
                     KDIIKSGVVGTFPVSTKFTHATGDYNVITGAYLGNITLKTEGTLTISANGSWTYNGVVR
                     SYDDKYDFNASTHRGIIGESLTRLGAMFSGKEYQILLPGEIHIKESGKR"
     gene            complement(8190..8543)
                     /gene="cmi"
                     /locus_tag="VE62_RS00380"
     CDS             complement(8190..8543)
                     /codon_start=1
                     /gene="cmi"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104216667.1"
                     /locus_tag="VE62_RS00380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="colicin M immunity protein"
                     /protein_id="WP_000864812.1"
                     /transl_table=11
                     /translation="MKVISMKFIFILTIIALAAVFFWSEDKGPACYQVSDEQARTFVKN
                     DYLQRMKRWDNDVQLLGTEIPKITWEKIERSLTDVEDEKTLLVPFKAEGPEGKRMYYGM
                     YHCEEGYVEYAND"
     misc_feature    complement(8513..8527)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 22.3"
                     /tool="antismash"
     misc_feature    8571..8587
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 25.24"
                     /tool="antismash"
     misc_feature    complement(8577..8586)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.31"
                     /tool="antismash"
     gene            complement(8716..>8874)
                     /locus_tag="VE62_RS00385"
                     /pseudo=""
     CDS             complement(8716..>8874)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237314.1"
                     /locus_tag="VE62_RS00385"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="tyrosine-type recombinase/integrase"
                     /pseudo=""
                     /transl_table=11
                     /translation="PLKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFSMPESDAVT
                     MLKNRHA"
ORIGIN
        1 cccgtatcac tgccctcagg gcaggctaac cgtgaggaaa gctgtcagtg atgcgggtac
       61 aggagtgcgg agaaagcgaa tgccatcctg taatgggatg gacaggaatt gagctgtttc
      121 agcaacatca ttccacgcga aagcgagcag acttccgcat ggtctttcaa cacgagataa
      181 cctgaatacc tttgaaggag ggtaagcaga tgactgcgaa aaacataaac gcgggtgcac
      241 ccaccgacat cgtgactcac tggcacaata tcaactgggc caggtgccgt cgcgaggtca
      301 gaaggctgca ggcgcgtatc gtgaaggcaa cacaggagga gaaagcgggc aaggtaaaag
      361 ccttgcaatg gctactcacc cactcgttca gcgccagagc aatggcagtt aaacgagtga
      421 cagataacag agggaagaga acaccgggcg ttgatggaaa aacatggtca aaacccggca
      481 gcaagatgaa ggctatatac acactaaaaa gaaggggtta caaacctctt ccgctacggc
      541 gtatatatat cccaaaatct aacggcaaaa agcgaccatt ggggatcccg acaatgaaag
      601 acagagccat gcaggcactc tacctgatgg cgctggaacc tgttgcagaa acaacggctg
      661 acccaaattc gtttgggttc aggccctgtc gctcaacagc cgatgccatt gagcaatgtt
      721 tcacaacgtt gcacagggct gatcgggcac aatggattct ggaagccgac attcgcagtt
      781 gttttgatga gatttcacat gaatggctaa tcgccaatat cccaacggat acggcgatcc
      841 tgaaacgatg gctgaaagcc ggatatattg atttggggaa attatatcca accagtgcag
      901 gtactccaca gggcgggata atatctccaa cgctggcaaa catggttctg gatggtctgc
      961 aacctctgtt gaagaaaacc ttctatcgaa gcggactgaa tcctgaaaag atcaacataa
     1021 tccgttatgc ggacgatttt gtgatcacgg ggataagcca cgatactctg tcagaaaagg
     1081 ttcttccctt gttagaaaac tttctggcag ataggggact tactctctcg cctgaaaaga
     1141 cgcgaatcac acacatcagt gatggattcg actttctggg tatgaacatc cgtaaatata
     1201 atgggaaact gttgattaaa ccttcgcccc aaaaggtaaa ggaattcctg aaaagggtcc
     1261 gccagatgat aaaagacaat ccgacagtca gccagagcac attgatatgg catctcaacc
     1321 caatcctcaa aggctgggcc agatactatc gtcatgttgt ctcaaaaaag atctttcaca
     1381 aagtcagtca tgaaatatgg aaagcactat ggcgctgggc atgtcgccgc cacccaaaga
     1441 aaggaacggg ctggataaga aggaaatact ttcgttcagt taacttccga cactgggtat
     1501 tcggcacaga gacagggaag atcctgccat caggcaaacg tgaattactg gcgttatatg
     1561 aaatctcgga tatgccaatc aaaagacaca gacaaatcaa aagcactgct aatccttttg
     1621 atcccagcta cgaatcgtac tttgaagcac ggctaaaggt tacgatgctg gaatccatac
     1681 ggggctactc tcgcttaaga aatctgtggc tccgacaggg aggtctatgc cccgtctgcc
     1741 gcctgccagt gaactccaca acaggctggc aacttcgaca cattgtgccg aaatcagaag
     1801 ggggactgga taacataggt aatctggcca tgttacatcc ggcatgtcat cacactgcaa
     1861 aacatcaggg aatatctgtt atgaagccgg ctctaatgtg gagttattga aaggcttgag
     1921 ccgtatgccg ggaaaccggc acgtacggtt cttagggggc agctgcacag caatgtgcag
     1981 ctgctacccg acatgaaatc gacagcattg cccggaaacg tggccataac acgcacgcag
     2041 tcaccctgga taaaacggga cataacagca ctccttctct tacagagggc accgggaggg
     2101 cgcccctgtg gcgcccgttc cccgctgcct gtgggctgtt caccgcgtcc ggtaacgggt
     2161 caggccgacc gtggaatacc gcagccgtcg tctttttgac ggcgaggata tgccgcgaaa
     2221 gcggcttgaa gggcgttcat cagaaaaggg cgacgggctg gcgtacactg tcaccaacgc
     2281 cagccgggag cggtaaaagc gcccgtcccg tgcccggccg gacgcgggac actgaccaca
     2341 ggcagcgttc ctgccgcgcc gcgtcagccg gcgcagtaaa aagaggagcc cggcgacatc
     2401 ttttgttacc cgccaaacaa aacccaaaaa caacccaaac caaacccaat aaaacaccaa
     2461 aacaagacaa ataatcattg attgatggtt aaaatggggt aaacttgaca aataaaccca
     2521 cttaaaaccc aaaacacacc caaatacaca ccaaaaaaca ccataaggag ttttataaat
     2581 gttggtattc attgatgacg gttcaacaaa catcaaacta cagtggcagg aaagcgacgg
     2641 aacaattaaa cagcacatta gcccgaacag cttcaaacgc gagtgggcag tctcttttgg
     2701 tgataaaaag gtctttaact acacactgaa cggtgaacag tattcatttg atccaatcag
     2761 cccggatgct gtagtcacaa ccaatatcgc atggcaatac agcgacgtta atgtcgttgc
     2821 ggtgcatcac gccttactga ccagtggtct gccggtaagc gaagtggata ttgtttgcac
     2881 acttcctctg acagagtatt acgacaggaa taaccaaccc aatacggaaa atattgagcg
     2941 taagaaagca aacttccgga aaaaaattac attaaatggt ggggatacat tcacaataaa
     3001 agatgtaaaa gtcatgcctg aatctatacc agcaggttat gaagttctac aagaactgga
     3061 tgagttagat tctttattaa ttatagacct cgggggcacc acattagata tttctcaggt
     3121 aatggggaaa ttatcaggga tcagtaaaat atacggagac tcatctcttg gtgtatctct
     3181 ggtcacatct gcagtaaaag atgcactttc tcttgcgaga acaaaaggaa gtagctatct
     3241 tgctgacgat ataattattc acagaaaaga caacaactat ctgaagcaac gaattaatga
     3301 tgagaacaaa atatcaatag tcaccgaagc aatgaatgaa gcacttcgta aacttgagca
     3361 acgtgtatta aatacgctca atgaattttc tggttatacg catgttatgg ttataggcgg
     3421 tggcgcagaa ttaatatgcg atgcagtaaa aaaacactca cagattcgtg atgaacgttt
     3481 tttcaaaacc aataactctc aatatgattt agttaacggt atgtatctca taggtaatta
     3541 ataatggaca agcgcagaac cattgccttc aaactaaatc cagatgtaaa tcaaacagat
     3601 aaaattgttt gtgatacact ggacagtatc ccgcaagggg aacgcagccg ccttaaccgg
     3661 gccgcactga cggcaggtct ggccttatac agacaagacc ccagggcccc tttcctttta
     3721 tgtgaactgc tgacgaaaga aaccacattt tcagatatcg tgaatatatt gagatcgcta
     3781 tttccaaaag agatggctga ttttaattct tcaacaatca ctcaaccctc ttcacaacag
     3841 gagcaaagaa gtgatgaaga gacaaaaaaa aacgcgatga agttaataaa ctgattaaat
     3901 ttttattgat atctttaccc ccataaacgg ggtaaaggta tcaacaaacc tattttttag
     3961 ctatgtacag attacatctg acaagttatt tctacgtaac aaatcacagt ctaaaaatca
     4021 gtatagcttt accctttgca ctgttcagct tattcctttc agaaaaaaaa cgggaacatc
     4081 agttacttta taaatctgca taataagtag tattctatgc ctgccatcta taattacttt
     4141 cccgtcttcg gacatttgtg gatattcaat cgttccttga cttaaatagg ttttaatttt
     4201 gtgccatcgg cgtggatcga tattgaaagc ctgaacgcca agcgacctta acagggttaa
     4261 cccattaatc atcgtgacca caggcttttg tctcattctc tcaggaagtt gatggcaatt
     4321 agcagccagg gccgccgggg gaaaataaaa ctccagttcc tgtagtgaag ggtattgcaa
     4381 cccactgttg tccagaggaa tatcatcaaa ggatttgaaa ggatacctgg acatatacgc
     4441 attgattttt tcaggcggta aatgtgcaaa ccagttagga tcaggcctgt tcgcttcttc
     4501 ttcttcttct ttaattcgtt gaaatgactc cggaaacaaa tcttccacac ttatgttcat
     4561 cacaatatcc tcccttgaga gcattgctca tcgccaccga taaggatttc attataaaac
     4621 acgccagtac agaacaactc actggtttca aactcccgtc gtccgtcctg accggtacgg
     4681 taactaacaa ggcctgtaca catatcacga tgtgtaccat gccataaagt gacagtatcc
     4741 ccccaggtgt ctcatatcat agtttcatta aaacatgctg aggccagaga acagttatgc
     4801 ctgtgccgtt ttttgtggta tcaggcgaga atagcacgtg gtgtgaatgc ctgttttttg
     4861 atcgttttca caaaaattga aatccacagt cttgacagag aaaacacagc ggcgtaattt
     4921 ttgatctgta cataaaacca gtggttatat gtacagcatt taattttaat tgattgtttt
     4981 taaagtcaaa gaggttttct atgagtgata atgaaggtag tgtaccgaca gaaggtattg
     5041 attacgggga cacaatggtt gtgtggccgt caacaggacg aattccgggc ggtgatgtga
     5101 aacccggagg ctcatcaggt ctcgctccat ccatgccttc gggatggggg gattacagcc
     5161 cacaaggtat cgcacttgta caaagtgttc tttttcctgg aattattcgc cggattattc
     5221 ttgataagga acttgaagag ggagactggt cgggatggtc tgtcagtgtg catagcccct
     5281 ggggaaacga gaaagtttcc gctgcacgaa cagttcttga aaatggttta cgtggtggtt
     5341 tgccagaacc gtctcgcccg gctgctgttt cttttgcccg tctggagcct gcttccggaa
     5401 atgagcaaaa aattattcgt cttatggtta cacagcaact ggagcaggta acggatatcc
     5461 ctgccagcca gttaccagca gcgggtaata atgtaccggt aaaatatcgt ctgacggacc
     5521 ttatgcagaa tggtacacaa tatatggcta ttatcggagg tattccgatg acagtgccag
     5581 tagtggatgc cgttccagtt ccggaccgga gtcgtccggg aaccaatatt aaagatgttt
     5641 acagtgcccc tgtatcacca aatctaccgg acctggtatt aagtgtgggt cagatgaata
     5701 ctccagttcg gtctaatccc gaaatccagg aagatggcgt tatttctgag acagggaatt
     5761 atgttgaggc tggttatacg atgtccagta ataatcatga tgtcattgtc cgttttcctg
     5821 aaggcagtgg agtttctccg ctatatattt cagccgtgga gattctggac agtaatagtt
     5881 taagccagcg ccaggaagcc gaaaataacg caaaggatga cttcagagtc aagaaagaac
     5941 aagaaaatga cgagaagacg gtcctgacaa aaaccagcga ggtcatcatt agtgtcggtg
     6001 acaaagtcgg ggaatatctt ggagataaat acaaggcgct ttcccgtgaa attgcagaga
     6061 atataaataa ttttcaggga aaaacgattc gtagttatga tgatgcaatg tcttccatta
     6121 ataagttaat ggctaacccc agccttaaaa taaatgcaac ggacaaagaa gccattgtga
     6181 atgcgtggaa agcatttaat gctgaggata tggggaataa atttgctgcg ttgggtaaaa
     6241 cgttcaaagc agcagattat gcaataaagg caaacaacat cagggagaag agtattgagg
     6301 gttaccagac tggtaactgg gggccattaa tgctggaagt cgagtcctgg gttatcagtg
     6361 ggatggcatc tgctgtagct cttagtttgt tttctttgac attaggctcg gcccttatag
     6421 cctttggtct ttcgtccaca gttgttggtt ttgttggcgt agttattgca ggtgctattg
     6481 gtgcatttat cgatgataaa tttgttgatg agttgaatca caagatcata aaataattaa
     6541 aaggaaggca gtgttactgc ctccctttaa aatatttaat gacaagcaat ggcatcagta
     6601 aaatcgcata tgtcatggtt aaaacagtaa agtaaatgga acaatataaa ataaacagag
     6661 tgatgacatt atgagataat aatttaaaaa aaccacctgc tgttgttagt tccagattat
     6721 agaacagtgt gatataaatc agaatggcat aaaaaacggg cccccagaaa caggattgca
     6781 caacaagttt tcttgttact ggctttatga tttctcttgt cttatatgtg caaagaaaaa
     6841 cgagaatacc cataactggt gctgtcttgt tgtagtttga cataagtgtt gtcatgacgg
     6901 gatcatgcaa tgatgtaata gaagggagga atgccacctt attatataag tagtacagca
     6961 cttgagaata tggattattt attcgaagta ttaatatagc cattaatgga ataatcccga
     7021 ttatggaaaa tgcatataat atttcgtttg ctttcttgtt cttatcttta ttgctggtca
     7081 tatatatacc tttttgtatt tttatatata gattatatat cagagttctc aatatatttc
     7141 cagtatttaa tggagtgtac agatatatat cttatatatg gtagttcagg tgcttcccta
     7201 ccatttttaa cggcatatcc ggcaaacaat gttatgggga gcctctctca tggggttcgt
     7261 tcgccggtgt aagcgttagc cagccgcgaa ctctgttatc ttgttaactt ataaggagtt
     7321 atgtatggaa accttaactg ttcatgcacc atcaccatca actaacttac caagttatgg
     7381 caatggtgca ttttctcttt cagcaccaca tgtgcctggt gctggccctc ttttagtcca
     7441 ggttgtttat agttttttcc agagtccaaa catgtgtctt caggctttaa ctcaacttga
     7501 ggattacatc aaaaaacatg gggccagcaa ccctctcaca ttgcagatca tatcgacaaa
     7561 tattggttac ttctgtaacg ccgaccgaaa tctggttctt caccctggaa taagcgttta
     7621 tgacgcttac cacttcgcaa aaccagcgcc aagtcaatat gactatcgct caatgaatat
     7681 gaaacaaatg agcggtaatg tcactacacc aattgtggcg cttgctcact atttatgggg
     7741 taatggcgct gaaaggagcg ttaatatcgc caacattggt cttaaaattt cccctatgaa
     7801 aattaatcag ataaaagaca ttataaaatc tggtgtagta ggtacattcc ctgtttctac
     7861 aaagttcaca catgccactg gtgattataa tgttattacc ggtgcatatc ttggtaatat
     7921 cacactgaaa acagaaggta ctttaactat ctctgccaat ggctcctgga cttacaatgg
     7981 cgttgttcgt tcatatgatg ataaatacga ttttaacgcc agcactcacc gtggcattat
     8041 cggagagtcg ctcacaaggc tcggggcgat gttttctggt aaagagtacc agatactgct
     8101 tcctggtgaa attcacatta aagaaagtgg taagcgataa tataagggcc ggggttccgg
     8161 cctttttatg cagcctttat aacagtgtat tagtcattcg catattcaac atatccctcc
     8221 tcacaatggt acatgccata atacattctc ttaccctccg ggccttcagc tttaaatggg
     8281 acaagaagtg ttttttcatc ttcaacatct gttaaacttc tctcaatctt ttcccatgta
     8341 attttcggga tttctgtacc aagaagttgt acatcgttgt cccagcgttt cattctttgc
     8401 aggtaatcat tttttacaaa cgttctggcc tgttcatcgc tgacctgata gcatgccgga
     8461 cctttatctt cagaccagaa aaaaacagca gcaagagcaa taatcgttaa aataaaaata
     8521 aatttcatgc taattacttt cataaaatct ccacaacttc tgtcgttcat tcgatataaa
     8581 aaaacattac gaatatatcc gtacaatgtg agcatactgt gaagtggctt taacaattat
     8641 gaaataatac aagagatcac atattgcagt ggtctctttt ttatagaaga aatcacaatt
     8701 cataattgtg acttattatg catgtctgtt tttcagcatt gtgaccgcat cggactcagg
     8761 catcgaaaac tgcacccggt gccgtgcagc cacatccagt gcaaacacct tcgtgtagac
     8821 ctccgttgag ctgatggact tatgccccat caggctctgc aggaccttca gcgg
//
