LOCUS       NZ_CP026498            11404 bp    DNA     linear   CON 22-MAY-2024
DEFINITION  Streptomyces sp. CB01881 chromosome.
ACCESSION   NZ_CP026498
VERSION     NZ_CP026498.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. CB01881
  ORGANISM  Streptomyces sp. CB01881
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 11344947)
  AUTHORS   Liang,H.
  TITLE     A 3-hydroxy-3-methylglutaryl-CoA synthase gene based probe for the
            discovery of the acyltransferase-less polyketide synthases
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11344947)
  AUTHORS   Liang,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2018) Xiangya International Academy of
            Translational Medicine, Tongzipo, Changsha, Hunan 410000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002953255.1-RS_2024_05_22
            Annotation Date                   :: 05/22/2024 02:39:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,978
            CDSs (total)                      :: 7,904
            Genes (coding)                    :: 7,565
            CDSs (with protein)               :: 7,565
            Genes (RNA)                       :: 74
            rRNAs                             :: 5 (5S)
            complete rRNAs                    :: 5 (5S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 339
            CDSs (without protein)            :: 339
            Pseudo Genes (ambiguous residues) :: 0 of 339
            Pseudo Genes (frameshifted)       :: 124 of 339
            Pseudo Genes (incomplete)         :: 251 of 339
            Pseudo Genes (internal stop)      :: 10 of 339
            Pseudo Genes (multiple problems)  :: 45 of 339
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:44:32
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 9843595
            Orig. end                         :: 9854999
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP026498.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11404
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="38"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11404
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11404
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6404](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6404)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            453..3206
                     /locus_tag="C2142_RS41770"
                     /old_locus_tag="C2142_32435"
     CDS             453..3206
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="C2142_RS41770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="C2142_32435"
                     /product="hypothetical protein"
                     /protein_id="WP_153882838.1"
                     /transl_table=11
                     /translation="MSVSPTRPPGPAVPAALAPRRLFGRRPRAGEPQLPQPVPPPSAQP
                     SAAPCGAELRLVGAHFVIAPASADDDELSAAVGSLPPLPGSLIVVAAAPDAAVVLRDRM
                     ADLGVVARARGAGTLVVAASGLAALAPNGRRPAELVAEQAGVPVVAPDGLVRITPDGGL
                     ALTTPDGAPEPAAWWHCVPGAAPQRLAGTPGATTAEPRATTHAAVRTATPPAARTAPAA
                     PPARTSGSPAAQVLRLPAGFWLTHPTAPPAGPLPGLGLAAAPPGTVVLLVGTPAAPLLL
                     PEEFADAAVALPLDAARLLVSAPWASPDELTALTGALAARLARPVHAAIGLPVLGEGGP
                     TSRVLDAQGQARWEPYLLRLASAPGQGTTPAAWRNSGADWRTDGPAVFAAFPYWALEAV
                     PAGLWLRPEPPHVLTPRFRRPEPARPLLIVGERDRPVEPDVFEELGALLDRLPTVGAEG
                     FGLLVHGLLEPSAERVGRFVARMHGLEWLGPDPAVARPPGVVPVSYGALPGAAPTGAPR
                     AVLGTPGAPAVLPAPGPAHLPAPGVDPGPGRLALPASPAAPDPAPLPEPTPLPTPPAPT
                     AVTTSSSSSTSATTNSSPAQPSAPEEPEELEESEESEELEEPTPPPATAPDPAAEAPCE
                     PAPGPRPEPTPQPARRYRPEEFAAPVTVSSAADRETVRALLGEHYQRCTGRVDQAATRL
                     PALRPTAKDDIRPDLAAVLLYHGDTGVPVGRAELTATARAGASAPLAAFLRCLGSGLRR
                     LPSHHGVVLLAADPGLDPAGVVDHYPVGRELLEPAPVAGLTAPTAELPGVSVEFVVWSA
                     TGRRTTAFGSADDEPQAVFAPGTTFTVIGAAEADPSGRPARVLLRESAGLASADPAERN
                     HNALARLNGWLERRDLLAPAERRPVTRPERFHLTPGIELPSAAGTV"
     gene            3376..4266
                     /locus_tag="C2142_RS32385"
                     /old_locus_tag="C2142_32440"
     CDS             3376..4266
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="C2142_RS32385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="C2142_32440"
                     /product="MinD/ParA family protein"
                     /protein_id="WP_148646368.1"
                     /transl_table=11
                     /translation="MRTASPAPAPAAARVPASAPGRRRSALDRLRSGERRCREHLRLIR
                     SPLQGSCRVAVVGAKGGAGRTSLVLTLGAMLAAERGEPVAAVDAGSHSGSLGRRIRRRT
                     EAGIADLAAARHPADLDRYVSPTPSGLAVLAGPDRPESLSDAGYVSALDLLAQRFPITL
                     TDSGTGLLDDPMRGVLGLADQLILATTPGVDSAGSAAATLDWLHAHGYGELAARSVTAI
                     SAVRGTVRPVGSAELRAYFGARCRGVVELPFDEHLAAGAEFDPGRLGLRTRAAVRELAA
                     LVGADLWRAGRPTGR"
     gene            complement(4297..4803)
                     /locus_tag="C2142_RS32390"
                     /old_locus_tag="C2142_32445"
     CDS             complement(4297..4803)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091728.1"
                     /locus_tag="C2142_RS32390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C2142_32445"
                     /product="DinB family protein"
                     /protein_id="WP_148646369.1"
                     /transl_table=11
                     /translation="MATERIAPPLTGGEREMLRAFLDYHRATLAMKCDGLTDDELRRQS
                     SPPSTLSLLGLVRHMAEVERTWFRRVINKEDIAFVWSPERDFQAAYDARDADRAQAFAA
                     WEEEVEHARRIEREAESLDVTGYMPKWEADVSLRLVMLHLIHEYARHNGHADFLRESID
                     GEVGA"
     gene            complement(5001..6404)
                     /locus_tag="C2142_RS32395"
                     /old_locus_tag="C2142_32450"
     CDS             complement(5001..6404)
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF016970.4"
                     /locus_tag="C2142_RS32395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C2142_32450"
                     /product="DUF692 family multinuclear iron-containing
                     protein"
                     /protein_id="WP_148646370.1"
                     /sec_met_domain="DUF692 (E-value: 4e-32, bitscore: 110.3,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGLGVGLTHVPGAEPILEACADLLEVVEIEPQALWRARPDGTTG
                     CDEEALRRLADLPGARLLHGVGNPVGGCRPPDPRHTALVGELAGRLRAPWVSEHLAFNH
                     TGRGDEGFRTGFMLPPRQTPEGVRRSVASVRAMADAVPVPLAVEIGANYLRPRADELSD
                     GRFAAGVAEGAGCGLLLDLHNVLANERNGRAPVARLLDELPLDRVWEVHLAGGFEHRGY
                     WLDAHSGLPDEELLALAERVLPRLPALRAVLFEVTGSAVPGLDPQAVRDLLVRVREQWR
                     PQEPMAPVPRERPAAGAPPAGPDTGPGPEEWEHVLGALTIGRVVDSPFARELAADPAVA
                     LLRELVTESRASALAGTLVHTVRLLLLTLGEAGVRDLLDDYTRSHPPELFATDEAAAFA
                     GHLRRTRPRVPWLADVLELDLGLVRVRLDDTPRTVRLGTDPNDLLAALGAGRLPVDPGR
                     GSFLVRLTA"
     gene            complement(6411..6665)
                     /locus_tag="C2142_RS32400"
                     /old_locus_tag="C2142_32455"
     CDS             complement(6411..6665)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="C2142_RS32400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="C2142_32455"
                     /product="hypothetical protein"
                     /protein_id="WP_148646371.1"
                     /transl_table=11
                     /translation="MSNNGTTRGPVPDPWLTGREVNVTVPATVAGDLDRMSGVLRSVLT
                     QLGCPTCHSGFDLRFRQSLDFVVSPKGDVAPGIAQPFVG"
     gene            complement(6977..7660)
                     /locus_tag="C2142_RS32405"
                     /old_locus_tag="C2142_32460"
     CDS             complement(6977..7660)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018547162.1"
                     /locus_tag="C2142_RS32405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C2142_32460"
                     /product="Tat pathway signal sequence domain protein"
                     /protein_id="WP_148646372.1"
                     /transl_table=11
                     /translation="MQTRTCLALAAVAVTLAVGAAPASASPGGTVATAAVLTTGGAGGA
                     PVAVGDVLAAPLASNTRATFYSTAAGSTGVTCGVSQLTARVLDNPAAPGTATESLTGHT
                     FSSCTSNVTGVTSVRSLTVGNLPYTVSVGDSAGNPVTLTSGTAGPIQATAVLNSWFGTL
                     TCSYQLSGAFTGNADNAAHSLVFRNEHFVKSGGSGLCPADGYFSATYGPVADTSRAGSP
                     VVDVN"
     misc_feature    complement(7672..7692)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 18.65"
                     /tool="antismash"
     misc_feature    7786..7800
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.96"
                     /tool="antismash"
     misc_feature    7798..7811
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 17.06"
                     /tool="antismash"
     misc_feature    7799..7812
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.08"
                     /tool="antismash"
     gene            7950..8759
                     /locus_tag="C2142_RS32410"
                     /old_locus_tag="C2142_32465"
     CDS             7950..8759
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0032259 - methylation [Evidence IEA]"
                     /NRPS_PKS="Domain: oMT (73-181). E-value: 2e-15. Score:
                     47.7. Matches aSDomain: nrpspksdomains_C2142_RS32410_oMT.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010982088.1"
                     /locus_tag="C2142_RS32410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C2142_32465"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_148646373.1"
                     /transl_table=11
                     /translation="MNHDRIPATVRQTYGAGDLAMAASFAGGFINFGDWHGIDTRSPGP
                     EDRVRSQEQLYRRVLGAFPAPAEGLRLAEVGCGRGLGAALALREFRFAGVTGVDIHPDQ
                     VERARAVNAKALATFPDRLAYTLGAADELPFPDGSLDGVYSVEAAQHFRELTGFAREAA
                     RVLRPGGRLVVTTFFSSGPEVAERLSILLASFADGLDVAHPLKDFTADLADAGFGNVSA
                     ESIGEHVWPGLDRYLEDTVPPGHWARNFLTAWQSGLLDYHVVTADRT"
     aSDomain        8169..8492
                     /aSDomain="MT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_C2142_RS32410_oMT.1"
                     /domain_subtypes="oMT"
                     /evalue="2.00E-15"
                     /label="C2142_RS32410_oMT.1"
                     /locus_tag="C2142_RS32410"
                     /protein_end="181"
                     /protein_start="73"
                     /score="47.7"
                     /tool="antismash"
                     /translation="EVGCGRGLGAALALREFRFAGVTGVDIHPDQVERARAVNAKALAT
                     FPDRLAYTLGAADELPFPDGSLDGVYSVEAAQHFRELTGFAREAARVLRPGGRLVVTTF
                     FSSG"
     gene            complement(8949..9596)
                     /locus_tag="C2142_RS32415"
                     /old_locus_tag="C2142_32470"
     CDS             complement(8949..9596)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018537277.1"
                     /locus_tag="C2142_RS32415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C2142_32470"
                     /product="hypothetical protein"
                     /protein_id="WP_148646374.1"
                     /transl_table=11
                     /translation="MPAALWTGREAHPDRVTADLTGVLGRELGLARPPVAVTLPPDSTG
                     VPAGSLLPPRERFSGMPAPTLCYVYVDARAPRPFELRASLMAGRALVRRSLGLGQLFYA
                     VPLTRSVPARTALSAPRRFGPSSFEGDAGVAGRLNADRELVADANALTPLEAGPDASHT
                     WSVERLLAVEPLPGEQGSVLLLRTLHRAAAHGWSLRADAVLNLAARIEAVLG"
     gene            complement(9625..10020)
                     /locus_tag="C2142_RS32420"
                     /old_locus_tag="C2142_32475"
     CDS             complement(9625..10020)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018537278.1"
                     /locus_tag="C2142_RS32420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C2142_32475"
                     /product="hypothetical protein"
                     /protein_id="WP_148646375.1"
                     /transl_table=11
                     /translation="MDDLMHKLVSAALSGGLIALVGGLSVLVRRRRVARAEAAAPAPVE
                     DLSQTLLRQAREADRSRDELAGQGRPAEALDPARAAAELWRRLTEVRQGRFLAERQAAL
                     ARLGELLAAAGRPDEAARVRQEIVGLG"
     gene            complement(10233..11351)
                     /locus_tag="C2142_RS32425"
                     /old_locus_tag="C2142_32480"
     CDS             complement(10233..11351)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1104: putative esterase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007446228.1"
                     /locus_tag="C2142_RS32425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C2142_32480"
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="WP_148646376.1"
                     /transl_table=11
                     /translation="MTSILSGARVRAAMRATATAAATALAATVVALGGPVAHADAHADA
                     PAVPAFTDGYGLTQVVGATEVHSGTDFTITVTTPQLPGRHRIRILLPNGYAAEPGKRWP
                     VAYFLHGGGGNVDDAAAAPALHSDSMITVVPDGGLKGWYANWLMQNTALGTANWEAFHL
                     TQVVPFIDANLRTVADRGHRAVVGLSMGGYGALHYAEARPDLFGHTASLSGGIDFGMAE
                     IRAAVLATELNVTGAWCAVSTSSPAGTGRCTGYGPYTDSDAIFGSPYPVFNADHVWNEV
                     NPAAKANLAKLAGTEVTLYTGNQGPIDHFTEMAANNVKARLDQLGLPSRFVDYGNGTSL
                     APGCDGGHNYGCWAPVLADYIPRLQASFAAAG"
ORIGIN
        1 tcacccgagc gcgcgtgggc cggttcgttc gtagacgggt catccagggg cgaaccatcc
       61 cgccttcttc cggacggccg ggctcgggtc ctgggcgtgc gacgcctgca aggctgcgcc
      121 ggcccgggcg tcggtgcggg agaacgtgcc gccctggggt cggtgatgcc gcgggcggcg
      181 agttcggtgg tgatcagggc agccacctcg gcggcggtct gctgcgtcgg ggtcttgaac
      241 tcggccttcc accgcttgcc ggccggctgc cagccgtccg ccgtcatcgc cgccgcgacg
      301 gcctcccggg tcgccggggc gcgggccgac gcggtccacg aacccgcgcg gtccacgtcc
      361 ccgcacaccc gcacaccccc atgctcgcgc ggcccgcatg ctcgcgcgga cgaggtgaac
      421 ccccgaccgg gccccagccg tttaccagga cgatgagcgt ctctcccacc cgaccgcccg
      481 gccctgccgt ccccgccgcc ctggcgccgc gccggctgtt cgggcgtcgc ccgcgggcgg
      541 gtgaaccgca actcccccag ccggtgccgc cgccgtcggc gcagccgtcc gccgcaccgt
      601 gtggcgccga actccggctc gtcggagcgc acttcgtcat cgcccccgcc tcggccgacg
      661 acgacgagct gtcggcggcg gtcggctcgc tgccgccgct gccggggagc ctgatcgtgg
      721 tcgccgccgc ccccgacgcg gcggtcgtgc tgcgcgaccg gatggccgac ctcggcgtgg
      781 tcgcccgtgc ccgcggggcc ggcacgctcg tcgtcgccgc gtccgggctg gccgcgctcg
      841 cgcccaacgg ccgccggccc gccgagctgg tcgccgagca ggcgggcgta ccggtggtcg
      901 ccccggacgg actggtgagg atcaccccgg acggcggcct cgcgctcacg acccccgacg
      961 gcgcccccga gccggccgcc tggtggcact gcgtcccggg cgcggcgccg cagcggctgg
     1021 ccggaacacc cggcgccacc acggccgagc cccgggccac cacccacgcc gccgtccgaa
     1081 ccgccacgcc tccggctgcc cggaccgccc cggccgcccc gcccgcccgt acgtccggga
     1141 gccccgccgc ccaggtactg cgcctgcccg ccggtttctg gctcacccac ccgacggccc
     1201 cgcccgccgg cccgttgccc ggcctcgggc tggccgccgc accgcccggc accgtcgtcc
     1261 tgctggtcgg cacccccgcc gcgccgctcc tcctccccga ggagttcgcc gacgccgcag
     1321 tcgccctgcc cctcgacgcc gcccggctgc tggtcagtgc cccctgggcc tcacccgacg
     1381 aactcaccgc tcttaccggc gcgttggccg cccgactggc ccggccggtg cacgccgcga
     1441 tcggcctccc ggtgctcggc gagggcggtc ccacctcccg tgtcctggac gcgcaggggc
     1501 aggcccgctg ggagccgtac ctcctgcggc tcgcctcggc ccccggccag ggcaccaccc
     1561 cggcggcatg gcgcaacagc ggagccgact ggcggaccga cggaccggcc gtcttcgcgg
     1621 ccttcccgta ctgggcgctg gaggccgtcc cggccggcct ctggctccgc ccggaaccgc
     1681 cgcacgtgct caccccgcgc ttccgccgcc ccgaaccggc ccgcccgctg ctcatcgtcg
     1741 gcgagcggga ccgcccggtg gagccggacg tcttcgagga gctcggcgcg ctgctggacc
     1801 gtctgccgac cgtcggcgcc gaggggttcg gcctgctcgt acacggactg ctggagccgt
     1861 ccgccgaacg cgtcgggcgt ttcgtcgccc gcatgcacgg cctggagtgg ctcgggcccg
     1921 acccggccgt ggccaggccg ccgggcgtgg tgccggtctc gtacggagcc ctgcccgggg
     1981 cggcaccgac cggggcgcct cgcgccgtcc tgggcactcc cggtgccccc gccgtcctcc
     2041 ccgccccggg tcccgcacac ctcccggcgc ccggggtcga ccccggcccc gggcgcttgg
     2101 ccctgcccgc ttccccggcc gcccccgacc ccgcaccgct tcccgagccg actcccctgc
     2161 cgaccccgcc cgccccgacc gccgtcacca ccagtagcag cagcagcacc agcgccacca
     2221 ccaacagcag ccctgcgcag ccgtccgcac cggaggagcc ggaggaactt gaggagtcgg
     2281 aggagtcgga ggagcttgag gaaccgaccc cgccgcccgc aactgctcca gacccggccg
     2341 ccgaagcccc gtgcgaaccc gcccccggcc cccggcccga accgactccc caaccggccc
     2401 gccgctaccg cccggaggag ttcgccgccc ccgtcaccgt cagcagcgcc gccgaccgcg
     2461 aaaccgtccg ggccctgctc ggcgagcact accagcggtg caccggccgg gtggaccagg
     2521 ccgccacccg gctgcccgcc ctgcggccca ccgcgaagga cgacatcagg cccgacctcg
     2581 ccgccgtcct gctgtaccac ggcgacaccg gagtcccggt cggccgggcc gagttgactg
     2641 cgacggcccg ggccggagcg tccgccccgc tggccgcctt cctccgctgt ctcggatccg
     2701 gcctgcgccg cctcccgagc caccacggcg tcgtcctgct cgccgccgat cccggactcg
     2761 atccggccgg ggtggtggac cactatccgg tcggacggga gctcctggaa cccgccccgg
     2821 tcgccggtct gaccgcgccg acggccgaac ttccgggtgt gtccgtcgag ttcgtcgtct
     2881 ggtcggccac cggccggcgc accaccgcgt tcggcagcgc cgacgacgag ccgcaggcgg
     2941 tgttcgcccc gggcacgacg ttcaccgtca tcggggcggc ggaggccgac ccgtccggcc
     3001 gtccggcccg ggtcctgctg cgcgagtccg ccggcctcgc gagtgccgac ccggccgagc
     3061 gcaaccacaa cgccctggcc aggctgaacg gttggctgga gcggcgcgac ctgctggcgc
     3121 cggccgaacg acgcccggtc acccgccccg aacggttcca cctcaccccg ggcatcgaac
     3181 tcccctccgc agccgggacg gtgtgacccg gcgccgtcga actccggcct tccgaccccc
     3241 tcccgccctc gtcgcgagtg cgcggtcgtg ccccgccggg caccacctgg acgccctggc
     3301 actgaactga cctgcaccaa cgcgccgtag agcagggtgg agagtgcgcc gcagaccgcg
     3361 tccgaaggag acagcatgag gactgccagc cccgcccccg caccggccgc cgcccgcgtg
     3421 ccggcctccg cccccgggcg gcggcgcagc gcgctggacc gcctgcgctc cggcgaacgc
     3481 cggtgccgcg agcatctgcg gctcatcagg tccccgctgc aggggagttg ccgggtggcc
     3541 gtggtgggtg ccaagggcgg cgccggcagg acctcgctgg tgctcaccct gggcgcgatg
     3601 ctggccgccg agcgcggcga gccggtcgcg gcggtcgacg cggggtcgca cagcggctcg
     3661 ctgggtcgcc ggatccggcg gcgcacggag gccgggatcg ccgacctcgc ggccgcccgg
     3721 caccccgccg acctcgaccg gtacgtctcg ccgacgccga gcggactggc ggtgctcgcc
     3781 ggtcccgacc gtccggagtc gctctccgac gccggctacg tctccgccct ggacctgctg
     3841 gcgcagcggt tcccgatcac cctcaccgac tccggcaccg gcctgctgga cgacccgatg
     3901 cgcggggtcc tcggcctggc cgaccagttg atcctcgcca ccaccccggg cgtcgacagt
     3961 gcgggcagcg cggcagcgac cctcgactgg ctgcacgcgc acggctacgg ggagctggcc
     4021 gcccgttcgg tcaccgcgat ctcggcggtc cgcgggacgg tccggccggt cggatccgcc
     4081 gagctgcggg cgtacttcgg cgcccgctgc cggggcgtgg tcgagctgcc gttcgacgag
     4141 cacctggcgg cgggcgccga gttcgacccc ggccggctcg gcctccgcac ccgcgcggcc
     4201 gtgcgcgagc tggccgccct ggtcggcgcc gacctctggc gggcggggcg gcccaccggg
     4261 cggtgaaccg gcggggcccg gtctcgtccc tggcccctac gcccccacct cgccgtcgat
     4321 cgactcgcgc aggaagtcgg cgtgcccgtt gtgccgggcg tactcgtgga tcaggtgcag
     4381 catcaccagg cgcagcgaca cgtcggcctc ccacttcggc atgtagcccg tcacgtccag
     4441 cgactccgcc tcccgctcga tccggcgggc gtgctcgacc tcctcctccc aggccgcgaa
     4501 cgcctgcgcc cggtcggcgt cgcgcgcgtc gtaggcggcc tggaagtcgc gctccgggga
     4561 ccagacgaac gcgatgtcct ccttgttgat cacccggcgg aaccacgtcc gctcgacctc
     4621 ggccatgtgg cggaccagcc cgagcaggga gagcgtggac ggcgggctcg actgccgccg
     4681 cagctcgtcg tcggtcagcc cgtcgcactt catcgccagc gtcgcccggt ggtagtccag
     4741 gaaggcgcgc agcatctccc gctcgccccc ggtcagcggc ggcgcgatcc gttcggtggc
     4801 catggtggtc tcccctgttc atggtcggcg ggcccgctcc ggccccgccg ccccgtcgga
     4861 gccccagtct cccgcagccc cggacggcac cctccacggg gagcggccac gcggcgggcc
     4921 cgccggcggc cgccggccgg tggtccgcct ccccggcgga ccgtcaggcg gaccacccgg
     4981 aacgaccgtc aggcggaccg tcaggccgtc agccggacca ggaaactgcc ccggccgggg
     5041 tcgacgggca gccggccggc gccgagcgcg gccagcaggt cgttcgggtc ggtgccgaga
     5101 cggaccgtcc gcggggtgtc gtcgagccgc acccggacga ggccgaggtc cagctccagc
     5161 acgtcggcca gccaggggac gcgcggacgg gtgcgccgca ggtggccggc gaacgccgcc
     5221 gcctcgtccg tggcgaacag ctccggcggg tgggagcggg tgtagtcgtc cagcaggtcg
     5281 cgcaccccgg cctcgccgag ggtgagcaac agcagccgga cggtgtgcac gagcgtgccg
     5341 gccagcgcgc tcgcccggga ctccgtgacc agctcccgca gcagcgcgac cgccgggtcc
     5401 gccgccagct cgcgggcgaa cgggctgtcc accacccggc cgatcgtcag cgcacccagc
     5461 acgtgctccc actcctcggg gcccggtccg gtgtccgggc cggcgggagg cgcaccggcg
     5521 gcaggccgct cccgcggcac cggcgccatc ggctcctgcg gccgccactg ctcgcggacg
     5581 cggaccagca ggtcccggac ggcctgcggg tcgagacccg gcacggcgga gcccgtcacc
     5641 tcgaacagga ccgcccgcag ggccggcagc ctcggcagca cccgttccgc cagggcgagc
     5701 aactcctcgt ccggcagccc gctgtgcgcg tccagccagt agccgcggtg ctcgaagccg
     5761 ccggccaggt gcacctccca gacccggtcc agcgggagtt cgtcgagcag ccgggcgacc
     5821 ggtgccctgc cgttgcgttc gttcgcgagg acgttgtgca ggtcgagcag cagcccgcag
     5881 cccgcgccct cggccacgcc cgcggcgaac cggccgtcgg acagctcgtc ggcgcgcggc
     5941 cgcaggtagt tggcgccgat ctccacggcg agcgggaccg gcacagcgtc ggccatggcc
     6001 cggacggagg cgacgctgcg ccgcacaccc tcgggggtct gccgcggcgg gagcatgaag
     6061 ccggtgcgga agccctcgtc gccgcggccg gtgtggttga acgccaggtg ttcgctgacc
     6121 cagggcgccc gcagccgccc ggccagctcg ccgacgaggg cggtgtgccg ggggtcgggt
     6181 ggccggcagc cgccgaccgg gttgccgacg ccgtgcagca gccgggcccc gggaaggtcc
     6241 gcgaggcgcc gcagcgcctc ctcgtcgcag ccggtcgtgc cgtccggccg cgcgcgccag
     6301 agcgcctgcg gttcgatctc gacgacctcc agcaggtccg cgcaggcctc aaggatcggc
     6361 tcggcgccgg ggacgtgggt gaggccgacg ccgagcccgg tcacgtcggt ctaacccacg
     6421 aacggctggg cgatgcccgg ggcgacgtcg cccttggggg agaccacgaa gtccagcgac
     6481 tgccggaacc tcaggtcgaa cccggagtga caggtcggac agccgagctg ggtcagcacg
     6541 ctcctgagca ccccgctcat ccggtcgagg tcacccgcca ccgtcgcggg cacggtgacg
     6601 ttgacctcgc gcccggtcag ccacgggtcc ggcaccggtc ccctcgtggt cccgttgttc
     6661 gacatcgcgc ctcctcgctc cacgccgcgg ccccgtaccg gcggtcaccg gcacgtcctg
     6721 agccccggac ggggcacagc attccgcccc gccgagcggg ccgaggaggt acgcgcccga
     6781 actgtcaccc ggactggcta gaccggggcc ggcaccgtcc ccgccgctcc gcagcccccc
     6841 gccacgagcc gcccgcccgg ccgcgcgggg cggtcccgtc cgccactgcg cgcgcccgcc
     6901 cgaccgggcc gggtaccagg gctcggtacc agggctcggt gccgcgctcg gtgcgtgacc
     6961 gggcctgccg ggtcggtcag ttgacgtcga ccacggggct gcccgcccgg ctggtgtcgg
     7021 cgacgggccc gtaggtggcg gagaagtagc cgtcggcggg gcacaggccc gatccgccgc
     7081 tcttgacgaa gtgctcgttg cggaagacca ggctgtgggc ggcgttgtcg gcattccccg
     7141 tgaaggcgcc gctgagctgg tagctgcagg tgagggtgcc gaaccacgag ttcagcacgg
     7201 ccgtggcctg gatcggcccg gccgtcccgg aggtgagcgt gaccgggttg ccggccgagt
     7261 caccgacgga gacggtgtag gggaggttgc cgacggtgag gctccggacc gaggtgacac
     7321 cggtgacgtt gctggtgcac gagctgaagg tgtgcccggt cagtgactcc gtcgccgtcc
     7381 cgggcgcggc ggggttgtcg agcacccgcg cggtgagctg cgagacgccg cacgtcaccc
     7441 cggtcgatcc ggcggcggtc gagtagaacg tggcgcgcgt gttggaggcc agcggcgcgg
     7501 cgagtacgtc gccgaccgcg accggggcgc cgccggcccc gccggtggtc agcaccgccg
     7561 cggtggccac ggtgccgccg ggggacgcgg atgcgggggc ggcgccgacg gccagcgtca
     7621 cggcgaccgc ggccaatgcc agacaggtgc gagtctgcat ggggagcctc tttctcggca
     7681 ggggggcgag aagacgaacg tccggggctg tccgaacagt tgggggaatc ggtcagccca
     7741 ctacgttgta gagccgtgtc catgcaccgt caatccatcg cgcagcactt gctgacaatt
     7801 cgtcaacaaa aaggcccggc ggccccgccc gtactgccgt cccgaacccg cacaatccac
     7861 ccgcacgtgg cgttcggcca cgatcccgtt tgggtacgta ctgcggcgcc cccggagccg
     7921 gggcaacgac ccgcgaggag accgcgacca tgaaccacga ccgcattccc gcaaccgtgc
     7981 gacagaccta cggcgccggc gatctcgcca tggcggccag cttcgccggc ggcttcatca
     8041 acttcggcga ctggcacggg atcgacacca ggtcaccggg cccggaggac cgggtgcgca
     8101 gccaggagca gctgtaccgg cgggtgctgg gtgccttccc ggcgccggcc gaggggctgc
     8161 ggctggccga ggtgggctgc gggcgcgggc tgggcgcggc actggccctg cgcgagttcc
     8221 ggttcgccgg ggtgaccggg gtggacatcc atccggacca ggtcgagcgc gcccgtgcgg
     8281 tcaacgccaa ggcgctggcg acgttcccgg accggctggc ctacaccctc ggcgcggccg
     8341 acgaactacc cttccccgac ggctcgctgg acggcgtgta ctccgtcgag gcggcccagc
     8401 acttccgcga gctgaccggg ttcgcgcgcg aggcggcgcg ggtgctgcgg cccggcggac
     8461 ggctggtggt gaccacgttc ttcagctccg gaccggaggt cgccgagcgg ctgagcatcc
     8521 tgctcgccag cttcgccgac ggcctggacg tggcgcaccc gctcaaggac ttcaccgccg
     8581 atctcgcgga cgccggcttc gggaacgtct ccgccgagtc gatcggcgag cacgtctggc
     8641 ccggcctgga ccgctacctg gaggacaccg tcccgccggg ccactgggcg cgcaacttcc
     8701 tcaccgcctg gcagagcggc ctgctcgact accacgtcgt caccgccgac cgcacgtgag
     8761 cctgccgggc gtggacaccc tgcccggcgt ggacactctg ccgggcgtgg accggccggg
     8821 cggcccgcag cggcgacggc ccggggcggc tgcggggcgt cggcccagcc cccgccgccc
     8881 ccacagcccc tgcggccctc gtcgcccccg cagctccgta gccccgccgc cgcgccgccc
     8941 cgcacggctc agccgaggac ggcctcgatg cgggcggcca ggttcagcac ggcgtcggcg
     9001 cggagcgacc acccgtgagc ggcggcgcgg tggagggtgc gcaggaggag gaccgacccc
     9061 tgctccccag gcagcggctc gaccgccagc aaccgctcga ccgaccaggt gtgcgaggcg
     9121 tccggcccgg cctccagcgg ggtgagcgcg ttcgcgtcgg cgaccagttc gcggtccgcg
     9181 ttcaggcggc ccgcgacccc ggcgtcgcct tcgaaggacg acggaccgaa acgccggggc
     9241 gcactcaggg cggtacgggc gggcaccgag cgggtcagcg gcacggcgta gaagagctgt
     9301 cccagcccca ggctgcggcg gaccaacgcc cgcccggcca tgagcgaggc gcgcagttcg
     9361 aacggtctcg gcgcccgggc gtccacgtag acgtagcaga gcgtcggcgc gggcatcccg
     9421 ctgaaccgct cgcgcggcgg cagcaggctg ccggccggga cgccggtgct gtcgggcggg
     9481 agtgtgacgg ccacgggcgg ccgggcgagg ccgagttcgc ggccgagcac cccggtgagg
     9541 tcggcggtca cgcggtccgg gtgtgcctct cggccggtcc agagggcggc gggcatggtg
     9601 gtctcctcgc tccggtcgga ctcgtcagcc gagcccgacg atctcctgac ggacgcgcgc
     9661 cgcttcgtcc ggccggcccg cggcggcgag cagttcgccg agacgggcca gggcggcctg
     9721 ccgctcggcc aggaaccggc cctgccgcac ctcggtgagc cggcgccaga gctcggcggc
     9781 cgccctggcc gggtcgaggg cctcggccgg cctgccctgg ccggcgagct cgtcacggct
     9841 gcggtcggcc tcccgggcct gtcgcagcag ggtctgcgaa aggtcctcga ccggcgccgg
     9901 cgcggcggcc tcggccctgg cgacccgcct gcggcggacc aggacgctga gccccccgac
     9961 cagtgcgatc agcccaccgc tgagggcagc cgaaaccagc ttgtgcatca gatcgtccat
    10021 ggagtcctcg cttgctcgtg cgggccggag ccctggaagg aagtgcagtt cgggagggat
    10081 gggcggttcg ggagcgaagg gcggttcagg agggaagcgc agttcaggaa ggaagggcgg
    10141 aaggaagtgc ggttcgagag ggagtgggag ccgctccggc cgggccccgg cagtcggtca
    10201 ggccggggcc ctggccggat ccgctgccgc ggtcagcccg ccgcggcgaa cgaggcctgg
    10261 agccgcggga tgtagtccgc caggacgggc gcccagcagc cgtagttgtg gccgccgtcg
    10321 cacccggggg ccagcgacgt cccgttgccg tagtcgacga accggctcgg cagcccgagc
    10381 tggtcgaggc gcgccttgac gttgttggcg gccatctcgg tgaagtggtc gatcggcccc
    10441 tggttgccgg tgtagagcgt gacctccgtg ccggcgagct tcgccaggtt ggccttggcg
    10501 gcggggttga cctcgttcca gacgtggtcc gcgttgaaga cggggtacgg ggagccgaag
    10561 atcgcgtcac tgtcggtgta ggggccgtag ccggtgcacc gaccggtgcc ggccgggctg
    10621 gaggtgctca ccgcgcacca ggcacccgtg acgttgagtt cggtggccag gacggccgcg
    10681 cggatctcgg ccatcccgaa gtcgatgccg ccggacaacg aggcggtgtg gccgaacagg
    10741 tccgggcggg cctcggcgta gtgcagggcg ccgtagccgc ccatcgacag gccgacgacg
    10801 gcccggtggc cccggtcggc gacggtacgc aggttggcgt cgatgaaggg gacgacctgg
    10861 gtgaggtgga acgcctccca gttcgccgtg cccagggcgg tgttctgcat gagccagttc
    10921 gcgtaccagc ccttgaggcc gccgtccggc acgacggtga tcatcgagtc cgagtgcagg
    10981 gccggcgccg ccgccgcgtc gtccacgttg ccgccaccgc cgtgcaggaa gtacgccacc
    11041 ggccaccgct tcccgggctc cgcggcgtag ccgttgggca gcaggatgcg gatcctgtgc
    11101 cggccgggca gctgcggggt cgtcacggtg atcgtgaagt cggtgccgga gtggacctcg
    11161 gtggcgccga cgacctgggt caggccgtag ccgtcggtga aggccgggac ggcaggcgcg
    11221 tcggcgtgtg cgtcggcgtg tgcgaccggc ccgccgaggg cgaccaccgt cgccgccagc
    11281 gccgtcgcgg cggcggtcgc agtggcgcgc atcgcggcgc gcacgcgcgc accggacagt
    11341 atggatgtca tgaggggtct ctccgtcgag ggtgggtcgg gcgtgtcggc cgggtggtga
    11401 cccg
//
