LOCUS       NZ_CP026498            21050 bp    DNA     linear   CON 22-MAY-2024
DEFINITION  Streptomyces sp. CB01881 chromosome.
ACCESSION   NZ_CP026498
VERSION     NZ_CP026498.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. CB01881
  ORGANISM  Streptomyces sp. CB01881
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 11344947)
  AUTHORS   Liang,H.
  TITLE     A 3-hydroxy-3-methylglutaryl-CoA synthase gene based probe for the
            discovery of the acyltransferase-less polyketide synthases
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11344947)
  AUTHORS   Liang,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2018) Xiangya International Academy of
            Translational Medicine, Tongzipo, Changsha, Hunan 410000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002953255.1-RS_2024_05_22
            Annotation Date                   :: 05/22/2024 02:39:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,978
            CDSs (total)                      :: 7,904
            Genes (coding)                    :: 7,565
            CDSs (with protein)               :: 7,565
            Genes (RNA)                       :: 74
            rRNAs                             :: 5 (5S)
            complete rRNAs                    :: 5 (5S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 339
            CDSs (without protein)            :: 339
            Pseudo Genes (ambiguous residues) :: 0 of 339
            Pseudo Genes (frameshifted)       :: 124 of 339
            Pseudo Genes (incomplete)         :: 251 of 339
            Pseudo Genes (internal stop)      :: 10 of 339
            Pseudo Genes (multiple problems)  :: 45 of 339
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:44:32
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7273700
            Orig. end                         :: 7294750
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP026498.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..21050
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene-precursor"
                     /region_number="26"
                     /rules="(PT_FPPS_like and not PT_HPP)"
                     /tool="antismash"
     cand_cluster    1..21050
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_FPPS_like and not PT_HPP)"
                     /kind="single"
                     /product="terpene-precursor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21050
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:11050](+)"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..11050
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
     gene            complement(442..1218)
                     /locus_tag="C2142_RS21695"
                     /old_locus_tag="C2142_21735"
     CDS             complement(442..1218)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NTP_transf_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1064: glucose-1-phosphate
                     adenylyl/thymidylyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014136556.1"
                     /locus_tag="C2142_RS21695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C2142_21735"
                     /product="nucleotidyltransferase family protein"
                     /protein_id="WP_148644192.1"
                     /sec_met_domain="NTP_transf_3 (E-value: 8e-13, bitscore:
                     47.9, seeds: 297, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTADTTPPQAPQPPTPQQAVVTQAVILAGGQGSRLRPYTDDRPKP
                     LVEIPGTGTPILGHQLAWLASEGVTDVVISCGHLADVLEEWLDKADLPLRVRTVVEAEP
                     LGRGGGLKYAAKALPRPDEPWYATNGDIWTRFSLRDMAAFHHERGAVATLALARPRIPW
                     GAVETDQFGNVLDFIEAPPSPFLINAGLYVFAPEFTELLPDVGDHERTTFPQLARGKRL
                     AGYQLPQGVYWRAIDTAKDLTEAAKELASGAGRTPR"
     gene            complement(1267..1707)
                     /locus_tag="C2142_RS21700"
                     /old_locus_tag="C2142_21740"
     CDS             complement(1267..1707)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014627891.1"
                     /locus_tag="C2142_RS21700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C2142_21740"
                     /product="hypothetical protein"
                     /protein_id="WP_148644193.1"
                     /transl_table=11
                     /translation="MKQLVRRIGQTIALTLPVVLVTTGTLAVTRVPWAPPTGLDQQVVA
                     ASSGDGAGIGRASTGKAPEGLAPQDTLRVALLDDLRAHNPGTALDLLERSMREQPALTP
                     YCSTLATELGKAAVRKYQGDVQRARSFARPVCDGSFASGITG"
     gene            complement(1928..3019)
                     /gene="ugpC"
                     /locus_tag="C2142_RS21705"
                     /old_locus_tag="C2142_21745"
     CDS             complement(1928..3019)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ugpC"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004929896.1"
                     /locus_tag="C2142_RS21705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C2142_21745"
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="WP_148644194.1"
                     /transl_table=11
                     /translation="MASVTYDKATRVYPGADKPSVDALDLDIADGEFLVLVGPSGCGKS
                     TSLRMLAGLEDVNSGAIRIGDRDVTHLPPKDRDIAMVFQNYALYPHMTVAENMGFALKI
                     AGVNKAEIRAKVEEAGKILDLTALLDRKPKALSGGQRQRVAMGRAIVREPQVFLMDEPL
                     SNLDAKLRVSTRTQIAGLQRRLGITTVYVTHDQTEALTMGDRVAVLKDGLLQQVDTPRN
                     MYDKPANLFVAGFIGSPAMNLIEVPLVDGGVKFGGSVVNISREDLSGAGTDKTVTVGIR
                     PEHFEIVPGGGIEGVAVTVNVVEELGSDGFVYGTTKIGGEDKDLVVRVHGRQIPARGET
                     IHVVPIANETHVFSTSTGARLSN"
     misc_feature    3097..3112
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 23.23"
                     /tool="antismash"
     tRNA            3313..3389
                     /anticodon=(pos:7277047..7277049,aa:Thr,seq:tgt)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="C2142_RS21710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="C2142_21750"
                     /product="tRNA-Thr"
     gene            3313..3389
                     /locus_tag="C2142_RS21710"
                     /old_locus_tag="C2142_21750"
     gene            complement(3480..4313)
                     /locus_tag="C2142_RS21715"
     CDS             complement(3480..4313)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018562807.1"
                     /locus_tag="C2142_RS21715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_148644195.1"
                     /transl_table=11
                     /translation="MESPQPVEYAVAVERYLAAAGLGAASRRIYRIALDTWAWALADRP
                     APTGPGRRRARPPVLPLALLDHPDAPARLRAAAGHRAAGTDPRTLNRELSTLRAAVTWW
                     RAHGWIRTDPTAGLRPHPLPATADGPAPLTADQLRALFARRAPLREQVLWHLLHESGAA
                     IETVLALDADDLDLPRRRTLPSAPPLRWRAGTARLLPLLLAGRTTGPLFLTDRRAPATT
                     PAADRCPHTGRARLSYRRAAELFTTHTAHLAEPPATTWTLRQLRSDAGAPDLRSH"
     misc_feature    complement(4328..4345)
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 18.77"
                     /tool="antismash"
     misc_feature    4362..4377
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 15.75"
                     /tool="antismash"
     gene            4522..5829
                     /locus_tag="C2142_RS21720"
                     /old_locus_tag="C2142_21760"
     CDS             4522..5829
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138247.1"
                     /locus_tag="C2142_RS21720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C2142_21760"
                     /product="MFS transporter"
                     /protein_id="WP_148644196.1"
                     /transl_table=11
                     /translation="MAVDTERVPSRGQRSGGRLAVLGERDFRWFFVGYATSLFGSSMAP
                     VAVAFAVLDGGGGGTDLGWVMAARILPIVLVMLVGGVVADRLGSRRVMLVADVLRCGVQ
                     GVFAAALAAGHAPVWVMVGLVALWGLGEGVFLPALGALVPALVARKERLPDANTLLGLA
                     RSVSTVAGPATAGVVVAAYGPAAVLLVDAVTYGVGALALARLGVAAPEEPGEPESMLAD
                     LREGWSEFRSRPWLWITTVQMAFFNLLVWAPFLVLGPLTAQRALGGARGWGLVMGVYGA
                     GAVLGGLLMLGRRPVRPLAVATAATLGWALPSAALAAGLSLLWTAAVALVAGAGSAVCG
                     ALYNSTMQRWVPADVLARVTAFGGLGAFVLGPLGLAAAGPLADRVGAASVLGFGALWQL
                     VAGVVVLAVPAVSRRQWDEPAEPGAEGGRPGRAGRG"
     gene            5958..6191
                     /locus_tag="C2142_RS21725"
                     /old_locus_tag="C2142_21765"
     CDS             5958..6191
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="C2142_RS21725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="C2142_21765"
                     /product="hypothetical protein"
                     /protein_id="WP_148644197.1"
                     /transl_table=11
                     /translation="MPGSVTISHHESAVALDHADAKRLATVLEELAYLLEIPGPNRIND
                     AQLGALCEGRSPDRAELSHWSRGIAAELKGRL"
     gene            6404..6934
                     /locus_tag="C2142_RS21730"
                     /old_locus_tag="C2142_21770"
     CDS             6404..6934
                     /EC_number="2.1.1.63"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014136549.1"
                     /locus_tag="C2142_RS21730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C2142_21770"
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="WP_148644198.1"
                     /transl_table=11
                     /translation="MSWLTVETPLPSGPMHIAVTPLGVAAAGYLGHAGGELPHCTDESK
                     IAAVTDRIGAYLAGRSRELGLPIDWSLSTGPHRAVLQTLWRDVPFGRTITYGELAARSG
                     VFESVTEPGLAARTVGQMMAANPVALLVPCHRVVAADGIGGFGGGRVGIDVKRWLLTLE
                     GVLEPTLDWNGPL"
     gene            complement(6979..8073)
                     /locus_tag="C2142_RS21735"
                     /old_locus_tag="C2142_21775"
     CDS             complement(6979..8073)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010043006.1"
                     /locus_tag="C2142_RS21735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C2142_21775"
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="WP_148644199.1"
                     /transl_table=11
                     /translation="MSAPVRVWLNRTYAENVFFIEQLREAPRPVEVHATHVDPDSPVLA
                     AADHATLEPGDLTPDAYVGYALDYCARHSVDVFLPRLHQLAISLHQQEFEAIGTALVCP
                     PATAIAAFDSKADGYAAIAAAGLPTPPWWQVRTAADLIAAVELIEAAGLTPCLKPSSGA
                     GGEGFRVLTREPFGLHRLAGWDSRVQVDQVAAALEAAAPEGPAGAADLLVMPYLEGPEV
                     SVDCLADRDGTLLAAVGRSKHRRRRSFTTAPQYLEPARRVVESTRMAFLSNVQFRHLAG
                     EPVIIDVNTRPSGGLHQLRLCGLNLPWNAVQLALGERPDTDRAGELAGIDHTGIDYTLV
                     SGVRPVLPRQSPFGRGLTPVELVH"
     misc_feature    complement(8330..8344)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.91"
                     /tool="antismash"
     misc_feature    8331..8345
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.13"
                     /tool="antismash"
     misc_feature    complement(8335..8348)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.49"
                     /tool="antismash"
     misc_feature    complement(8384..8400)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 15.66"
                     /tool="antismash"
     misc_feature    8443..8456
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 12.5"
                     /tool="antismash"
     gene            8491..9912
                     /locus_tag="C2142_RS21740"
                     /old_locus_tag="C2142_21780"
     CDS             8491..9912
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008743711.1"
                     /locus_tag="C2142_RS21740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C2142_21780"
                     /product="family 2B encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_148644201.1"
                     /transl_table=11
                     /translation="MSTETNVGIPAPGGESPAQQSLGTAGARNLATTTKSAPQMQGISP
                     RWLLRMLPWVEVSGGTYRVNRRLRYAVGRGRVGFVKTGSEVRIVPPTLREVPVLRGFED
                     DAVLEELAGRFVQREFSAGEVLVAAGAPIDEVFIIAHGKLDRLGSGKYGDETVVGSLAD
                     GDHLGDEALRQEDGAWPYTVRAATAGTVLAISWPSFQELADRSEALQLQIMDYLAGFEQ
                     AQNKHGEAAIGLSAGHRGEYELPGAFVDYELKPREYELSVAQTVLKVHSRVADLYNQPM
                     NQTEHQLRLTIEALRERQEDELVNNPEFGLLQNAEYDQRIQTHSGPPTPDDMDELLSRR
                     RSTKMFLAHPRAIAAFGRECSRRGLYPDTVEVQGRQVFGWRGVPVFPCNKIPIVGGNTS
                     SIIALRTGEDNQGVIGLHQTGIPDEYQPGLSVRFMGISEKAIISYLVSAYYSAAILVPD
                     AVGVLENVDVAHHRD"
     gene            10001..11050
                     /locus_tag="C2142_RS21745"
                     /old_locus_tag="C2142_21785"
     CDS             10001..11050
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /GO_process="GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene-precursor: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145810.1"
                     /locus_tag="C2142_RS21745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C2142_21785"
                     /product="family 2 encapsulin nanocompartment cargo protein
                     polyprenyl transferase"
                     /protein_id="WP_148644202.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 7.5e-61, bitscore:
                     204.6, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGIFTLERDHREGHEAEDVLARARGLVDPVMRAAVDSLPGSMRKV
                     AGYHLGWWEADGTPVAAPSGKAIRPAMVLAAAQALGGTPAAATAAAAAVELVHNFTLLH
                     DDVIDRDETRRHRPTAWRVFGTTEAILVGDALHSLALRLLAGDTHPAAQGAIRRLTDCV
                     VELCEGQQIDCAFEQRSDVSLAECLAMAEAKTGALLGAACAMGAQYAGASEEAAQAMDG
                     FGREIGLAFQLIDDLIGIWGDPAVTGKPVGADLVARKKSLPVVAALGSGTADGHVLAGL
                     YALERPLSPEELNRCAAAVDGAGGRGWAQGESCERMATAMAQLAVAVPEPSAADELLAL
                     AELVTRRTH"
     gene            complement(11138..12208)
                     /locus_tag="C2142_RS21750"
                     /old_locus_tag="C2142_21790"
     CDS             complement(11138..12208)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /locus_tag="C2142_RS21750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C2142_21790"
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="WP_148644203.1"
                     /transl_table=11
                     /translation="MRILLLGGSSFLGRAFASEALARGHEVTTFTRGRSYADLPGVEAI
                     RGERDSADDLTQLVEGRHWDAVVDTSAQQPAQTVISARLLREHADRYTFVSSVHAFADW
                     PGQVVNEESPLHPCPADTPADQPFSNALKAGCERAVLEHFGAGRTLILNSGLLIGPYET
                     GGRLPWWLERMARGGPVLAPGDPERAMQVIDVRDFAAFGIGVVTAGHTGRYLTTAPAGN
                     VTFGGFLATCREVAGEPSDGRPRAELVWVEDGPLLAEGPDSVQPWLELPLWAPDIPEMA
                     AVWLADTARAQEAGLRCRPFAETARDTWAWLQQREPAEDTNADGLKGRKGAHHLQGIVP
                     AKEQRLLAALGTTDAE"
     misc_feature    complement(12194..12209)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.12"
                     /tool="antismash"
     misc_feature    complement(12414..12428)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.64"
                     /tool="antismash"
     misc_feature    complement(12422..12438)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 15.96"
                     /tool="antismash"
     gene            12466..13791
                     /locus_tag="C2142_RS21755"
                     /old_locus_tag="C2142_21795"
     CDS             12466..13791
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018961685.1"
                     /locus_tag="C2142_RS21755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C2142_21795"
                     /product="DUF1254 domain-containing protein"
                     /protein_id="WP_148644204.1"
                     /transl_table=11
                     /translation="MVNPALVDPATIRATAAEAWVRGYPILQNYRTLYAQAVDDADPRY
                     VGGFGVFRHYPQPFTPANTDIVTPNNDTPYSWAWLDLRAEPWVVSVPASDRYYVVPLHE
                     LDTVYAGFIGTRVTGQEAGDYLVAGPDWQGEAPEGIKGVIRTATRLIGIVGRTYLAGTS
                     PEDVEELERIQRQYQLRPLHEYAGTPAPPPAPHPVWPVWREEVMDSIEFLGFLDFLLGF
                     FPAPPAEADLRGRLTDLGVDGRGEFEPAALPLEVRAEIERGIADGRAELDRAARETSGS
                     TGLFGTREQVGTAYLKQALGAMEGLYGLPVEEAWYSGWLADSEGGRPPNASNHDYMLRF
                     APDRLPPARFFWSVTMYALPERLLVDNTLDRYSIGDRTPGLVRDADGGLTLYVQHKRPA
                     DAQHSANWLPAPDGPFTVVIRLYGPDESVLDGRWTLPPLTPR"
     gene            13942..15276
                     /locus_tag="C2142_RS21760"
                     /old_locus_tag="C2142_21800"
     CDS             13942..15276
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018511723.1"
                     /locus_tag="C2142_RS21760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C2142_21800"
                     /product="DUF1254 domain-containing protein"
                     /protein_id="WP_148644205.1"
                     /transl_table=11
                     /translation="MTQPELEALAADAYVYGYPLVSDLTMVERFTEGGMGILPAAPFNR
                     VSHAGRLVGPDDPFVSVNNDTVYSVAQLDLSEGPQVLHVPDTDGAYYVLQFVDAWTDNF
                     AYVGRRATGTGAQTWLIVPPGWHGTPTDHEHVIVSPTTVASIVGRFACTGPEDLPRVRA
                     LQEGLTLEPLEPGGLAAGLPGPDPEVPERLAFFERLRVWMAAFPPAAPDVDYQQRFAPL
                     GLLDTGPSPYRAAAPEWTLALLKGLAAGRERVEDAIRPPEDHPAGEWRTALHLFDYNID
                     FLGPGTIDEPDWRIPDRRAAYLSRAAAARAGLWGNHAYEAVYAMTSDDADGHPLNGAHT
                     YTLRFDQPPPADAFWSVTMYGLPEHYLVANPVGRYSIGDRTPGLVHGEDGSLTLRLQHE
                     QPEDPAGAANWLPVPEGGFRPIIRLYQPGPTVLDGTYRVPPIRRS"
     gene            15473..16144
                     /locus_tag="C2142_RS21765"
                     /old_locus_tag="C2142_21805"
     CDS             15473..16144
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003969909.1"
                     /locus_tag="C2142_RS21765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C2142_21805"
                     /product="GAP family protein"
                     /protein_id="WP_148644206.1"
                     /transl_table=11
                     /translation="MGDAVGQMLVSAMGIAISPMPLIAMILMLATPKGKANGLAFTAGW
                     VVALAAVVGVVVAAGSGLSAGSPKPAWAWWLKLALGVLFLLLGLRQWRDRPREGHVHGP
                     PKWMQAIDRFTPRKAAGLAAVLVAANPKNLVLAVGGAVSIATSTASGGGKAVAAALMVL
                     IGSLCTLLPLGVYLFGGDRSARVLGEWKAWMAAHNAAIMVVVLIVLGAKYTGDAISGLT
                     T"
     misc_feature    15478..15492
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.92"
                     /tool="antismash"
     gene            complement(16227..18338)
                     /locus_tag="C2142_RS21770"
                     /old_locus_tag="C2142_21810"
     CDS             complement(16227..18338)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019982497.1"
                     /locus_tag="C2142_RS21770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C2142_21810"
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="WP_148644207.1"
                     /transl_table=11
                     /translation="MTTSTEGPRDPVLAAYERQVDGLFTYCLSVLCEHDAAAAALREVR
                     ELALRHGTRLAEPGMVRAWLYSLARYSCLRRMGDGAGSGGGLPGAGAGPTGLVSSPTGV
                     LSGPTGVVSGSPGVPGSPGVSASPGVVSGPSGAGAGVPTAEAARRRRELASLAWPEAAG
                     TDPEQREALELSVRHRLTPIEVAAVLGVPFETSRALLASAEAEVGRTRTALLVLGVGSC
                     PELDRLGGAGAESWRDWVLGPALRRELVQHVVDCPTCRGTTERVAGEVTEGTTGLSGLP
                     VLTAPSAPAVRTAAPAGGAVRHGGVLFLPRATGAPAARRTAVPAPVVEPGLRFDQGGFP
                     RHRAPDARRGPAVRRRVVTTGVLAAVLAAPVVALWAGHRGGGGAGAAAAVSSVRVEDDQ
                     RAAGRRPGGSGGAGGEPGQAGQVGQVGGVVGAVGAAPAVAGLELAGAGPLRAETLLPGI
                     QGPAIPVPAHGATPLGSPTLVAAPAPVAGAAAGPGGGGPGAPGPAPAGLLTVEAGEYGN
                     RTVLTLTNSGVTEIRWHAEVDAAWLRLSREAGTLAPGQRITVIVTVDEDLAPGTHWTAR
                     IALPPSQAVVTLEGGPHHRGGSASAPAEPTAGPTSGPTSGPTSGPTAGPTSGPASPGGT
                     PSGGPSTAPGSPPPSPGTTASPTGAPSSPAPTRTPAPTGTASASPSSPPPSSSGGASAP
                     AATGSASPR"
     gene            18538..20049
                     /gene="radA"
                     /locus_tag="C2142_RS21775"
                     /old_locus_tag="C2142_21815"
     CDS             18538..20049
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /gene="radA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014136539.1"
                     /locus_tag="C2142_RS21775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C2142_21815"
                     /product="DNA repair protein RadA"
                     /protein_id="WP_148644208.1"
                     /transl_table=11
                     /translation="MAARTKTTAKPRPAYRCTECGNQLPKWVGRCPECNAWGTVEEYGA
                     VPIRTTAAGPVSAPARPIAQVDGQVATARSTGVPELDRVLGGGLVPGAVVLLAGEPGVG
                     KSTLLLDVAAKAASAQHRTLYVTGEESAGQVRLRADRINALSDHLYLAAESDLGAVLGH
                     IEAVGPGLLILDSVQTIASAELDGAPGGPAQVREVAGALIRASKERGMATLLVGHVTKD
                     GQIAGPRLLEHLVDVVLSFEGDRHARLRLIRGVKNRYGATDEVGCFELHDEGIVGLADP
                     SGLFLTRRDKPVPGTCLTVTLEGKRPLVAEVQALMVDSQIPSPRRTTSGLESPRIAMIL
                     AVVERHGGVKLGKQDIYTATVGGVKLSEPSADLAVALAVASSSTDTPLPSNLVAIGEVG
                     LAGEVRRVTGVQRRLAEAHRLGFTHALVPPDPGKVPPGMKVVEVADIGEALRAIPGRRR
                     AAAKPRGEAPSAPPTPRRAASAPPVAAHPEELMEGWEPLDSDELR"
     misc_feature    complement(20338..20353)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.61"
                     /tool="antismash"
     misc_feature    20380..20394
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.01"
                     /tool="antismash"
     misc_feature    complement(20588..20602)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 15.56"
                     /tool="antismash"
ORIGIN
        1 gggcggctcg ggggcggcgc ccaagccgag ccagagcacc ggctccggcc cggggctgct
       61 gggcggggtg ctgggcagcg ggccgctggc cgagctgtcc ggtgacggcg gccggagcac
      121 gcggccgggg tcgccgacgt ccacctgacg ggcggtcggg tccggcgggc cgtcggaccg
      181 cgtcgggccg tcgggcgcgc agggctcgcc gggcgcgtgc ggcgcgtgcg gctcgccggg
      241 aaacggatcg gccgggtgct ccgtggggga gcacccggcc gattgccgtg ccgtggccga
      301 ccgttgcggg ccgttccgtt cccggggctc tgtgcggggc ccggcgagca ccgtggggcg
      361 tcgtggggcc ccgctcaggg cgcggggtgc cgtcgggcgg tgctcagggc gcggggtgcc
      421 gcgggccgcc gtggggaccg gctaccgggg cgtgcggccg gctccggagg cgagctcctt
      481 ggcggcctcg gtgaggtcct tcgcggtgtc gatggcgcgc cagtagacgc cctgcggcag
      541 ctggtagccg gccagccgct tgccgcgggc cagctgcggg aacgtcgtcc gctcgtggtc
      601 gccgacgtcc ggcagcagtt cggtgaactc cggggcgaac acgtacaggc ccgcgttgat
      661 gaggaagggg gagggcgggg cctcgatgaa gtccagcacg ttgccgaact ggtcggtctc
      721 cacggcgccc cacggaattc gcggccgggc cagcgccagc gtcgcgacgg cgccccgctc
      781 gtggtggaag gcggccatgt cgcgcaggct gaacctggtc cagatgtcgc cgttggtggc
      841 gtaccagggc tcgtccggcc gggggagcgc cttcgccgcg tacttgagcc cgccgccgcg
      901 cccgagcggc tcggcctcga cgacggtgcg gacccgcagc ggcaggtcgg ccttgtcgag
      961 ccactcctcc agcacgtcgg ccaggtgccc gcaggagatc accacgtcgg tgacgccctc
     1021 ggaggcgagc caggccagct ggtggcccag gatcggcgta cccgtccccg ggatctcgac
     1081 cagcggcttc gggcggtcgt ccgtgtaggg ccgcaggcgc gagccctggc cgccggccag
     1141 gatgacggcc tgggtgacga cggcctgctg gggagtgggg ggctgcggag cctgcggggg
     1201 cgtggtgtca gcggtcatgc ctgcacaata ggcgtgtgct gccggtcgcc cgcagcggcg
     1261 gtggcgtcag ccggtgattc ccgacgcgaa cgagccgtca cagaccgggc gggcgaagga
     1321 acgggcgcgc tggacgtccc cctggtactt gcgcaccgcc gccttgccca gctcggtggc
     1381 gagcgtgctg cagtacggcg tcagcgccgg ctgctcgcgc atcgagcgct ccagcaggtc
     1441 cagcgcggtg cccgggttgt gcgcgcgcag gtcgtccagc agggcgacgc gcagtgtgtc
     1501 ctgcggcgcg agcccctccg gcgccttgcc ggtggacgcc cgcccgatgc cggcgccgtc
     1561 cccgctggac gccgccacga cctgctggtc caggcccgtc ggcggagccc acggcaccct
     1621 ggtcacggcg agggtgccgg tggtcaccag gaccacgggc agggtgaggg cgatggtctg
     1681 gccgatgcgg cgaactagct gcttcacgct caggagagta gcgccgagta cgcgccggat
     1741 cgccctccgt tacaccatcg agtgacaccg tgtcccggtt cgtcccgtcg ccgtgttgac
     1801 gaaggagcgt cgggcccgtg gaccggcggc ggaatcgcga cggagccccg ccggaccgca
     1861 cggtccgacg gggctccgtc ctgcgctctc ccccgcccgg cggctcgtcc gcccggcggg
     1921 tggtggctca gttgctcagg cgggcaccgg tgctggtgga gaacacgtgg gtctcgttgg
     1981 cgatcggcac gacgtggatg gtctcgccgc gcgccgggat ctgacggccg tgcacgcgga
     2041 cgaccaggtc cttgtcctcg ccgccgatct tggtggtgcc gtacacgaag ccgtccgagc
     2101 cgagctcctc gaccacgttc acggtgaccg cgacgccctc gatgccgccg cccgggacga
     2161 tctcgaagtg ctccgggcgg atgccgacgg tcacggtctt gtcggtgccg gcgccggaca
     2221 ggtcctcgcg ggagatgttg acgaccgagc caccgaactt cacgccgccg tcgaccagcg
     2281 gcacctcgat caggttcatc gcgggcgagc cgatgaagcc ggccacgaag aggttggcgg
     2341 gcttgtcgta catgttgcgc ggggtgtcga cctgctggag caggccgtcc ttcagcaccg
     2401 cgacccggtc gcccatggtg agcgcctcgg tctggtcgtg ggtgacgtac acggtggtga
     2461 tgcccaggcg gcgctggagg ccggcgatct gcgtacgggt cgagacgcgg agcttggcgt
     2521 ccaggttcga cagcggctcg tccatcagga agacctgcgg ctcgcggacg atcgcgcggc
     2581 ccatcgcgac acgctggcgc tgaccgccgg agagcgcctt cggcttgcgg tccagcagcg
     2641 cggtgaggtc gaggatcttc ccggcctcct cgaccttggc gcggatctcc gccttgttga
     2701 cgccggcgat cttgagcgcg aagcccatgt tctccgcgac ggtcatgtgc gggtacagcg
     2761 cgtagttctg gaacaccatc gcgatgtccc ggtccttcgg cggaaggtgc gtcacgtcgc
     2821 ggtcgccgat gcggatcgcg ccgctgttga cgtcttccag accggccagc atgcgcaggc
     2881 tggtcgactt gccgcagccc gaggggccga ccaggacgag gaactccccg tccgcgatgt
     2941 ccaggtccag cgcgtccacg gagggcttgt cggccccggg gtagacacgg gtcgccttgt
     3001 cgtacgtcac agaagccatg gctgtgctct ccttcaccgg caggaacgtg ccggacgatc
     3061 cgagtaaagg gtggatgtcg tgccgccgcc ccgccaactg gtctgaacca ctgagcgcac
     3121 cggctcccgg tgacgctacc tgggccctcg ctgcttgtca gcccccggcc atcccgattt
     3181 cggcatcggt tccatcacac ccgcccgccc tcacccggcc ccggccgtcc cccggatatg
     3241 ccagcaccgc cccacccccg cccggtagac ttcccccggt gccgccgtga ccatgtcgcg
     3301 gacggtcacc atgcctcctt agctcagctg gccagagcac cgctcttgta aagcgggggt
     3361 cgtcggttcg aacccgacag ggggctccag cagcgaacgc cccggaccag tgcggtccgg
     3421 ggcgttcgtc gttcccgggc cggcgcgggc cgtttcgccg aggggtctgt gcaccccggt
     3481 cagtgggatc ggaggtccgg ggcacctgcg tcggaccgga gttggcgcag ggtccaggtg
     3541 gtggcggggg gttcggcgag gtgggcggtg tgggtggtga agagttcggc ggcgcggcgg
     3601 taggagaggc gggcgcggcc ggtgtggggg cagcggtcgg cggcgggggt ggtcgcgggg
     3661 gcgcggcggt cggtgaggaa gagcgggccg gtggtccggc cggcgaggag cagggggagc
     3721 aggcgggcgg tcccggcccg ccagcggagg ggcggggcgg acggaagggt gcggcggcgg
     3781 gggaggtcga ggtcgtcggc gtcgagggcg aggacggtct cgatggcggc gccgctctcg
     3841 tgcagcaggt gccagaggac ctgttcgcgg agcggggccc ggcgggcgaa gagggcgcgc
     3901 agctggtcgg cggtgagcgg ggcggggccg tcggcggtgg cggggagggg gtgcgggcgc
     3961 aggccggcgg tggggtcggt gcggatccag ccgtgggcgc gccaccaggt cacggcggcg
     4021 cgcagggtgg agagttcgcg gttgagcgtg cgcgggtcgg tgccggcggc gcggtggccg
     4081 gcggcggccc ggaggcgggc gggggcgtcc gggtggtcga gcagggcgag ggggaggacg
     4141 ggcggccggg cgcggcgccg cccggggccg gtgggggcgg ggcggtcggc gagcgcccag
     4201 gcccaggtgt cgagggcgat ccggtagatg cgccgggagg cggcgccgag gccggcggcg
     4261 gccaggtagc gctcgacggc gacggcgtac tcgacgggct gggggctctc cacgggaatg
     4321 gctcttcttt ccgcagtaaa tgacatctgg gggaggggtt caagaatagc cattagccgc
     4381 agtaaacggc agtctgagct ggtggattgg cgatatttac tgcggcagac ccagcccgtc
     4441 gaagaattga tgcagaaaga ctcaactttt cctccggaca cccttgttca cggctcctcg
     4501 gcgggggcag acttgccgcc catggcagtg gatacggagc gggttccgtc gcgcgggcag
     4561 cggtcgggcg ggcgtctcgc cgtgctgggc gaacgcgatt tcaggtggtt cttcgtcggg
     4621 tacgcgacgt cgttgttcgg ctcctcgatg gcaccggtgg cggtcgcctt cgcggtgctc
     4681 gacggcggag ggggcgggac cgacctgggg tgggtgatgg cggcccggat cctgccgatc
     4741 gtgctggtga tgctggtcgg cggggtggtc gccgaccggc tcggcagccg gcgggtgatg
     4801 ctggtggcgg acgtgctccg ctgtggggtg cagggggtgt tcgcggcggc gctggcggcg
     4861 gggcacgcgc cggtctgggt gatggtcgga ctggtcgcgc tctgggggct cggcgagggc
     4921 gtgttcctgc cggcactcgg ggcgctggtg cccgcactgg tcgcccgcaa ggagcgtctt
     4981 ccggacgcca acaccctgct ggggctggcc cgttcggtgt ccacggtggc cgggccggcc
     5041 acggcgggcg tggtggtggc ggcgtacggc ccggcggcgg tgctgctggt cgacgcggtc
     5101 acgtacggcg tcggcgcgct ggcgctggcc cggctcgggg tggccgcacc ggaggagccg
     5161 ggggagccgg agtcgatgct ggcggacctg cgcgaggggt ggtcggagtt ccgctcccgg
     5221 ccgtggctct ggatcaccac ggtccagatg gcgttcttca acctgctggt ctgggcgccc
     5281 ttcctggtgc tcggcccgct gaccgcccag cgggcgctcg gcggcgcgcg cggctggggc
     5341 ctggtgatgg gcgtgtacgg ggccggcgcg gtgctgggcg gcctgctgat gctgggccgc
     5401 aggccggtgc ggccgctcgc ggtggccacg gcggcgaccc tcggctgggc gctgccctcg
     5461 gcggcgctcg cggcgggcct gtcactgctc tggacggcgg cggtggcgct ggtggccggg
     5521 gccggttcgg cggtctgcgg ggcgctgtac aactccacga tgcagcgttg ggtgccggcc
     5581 gacgtcctcg ccagggtcac cgcgttcggc gggctcggcg cgttcgtgct cgggccgctc
     5641 ggcctggcgg cggccggccc gctggcggac cgggtgggtg cggcgtccgt gctcggcttc
     5701 ggcgcgctgt ggcaactggt cgcgggcgtc gtggtgttgg cggttccggc ggtgagccgg
     5761 cggcagtggg acgaacccgc ggagcccggc gccgagggcg ggcgccccgg ccgggcgggc
     5821 agggggtgac gcatgctgcc gcagcgccgg gcgctgcgcg aggcgcccgc ccggtcaccc
     5881 ggggtgagcg ggcgcgcagg gcggcccggg cggtcgtacg gtggagggag tgcgggggcg
     5941 aacggaggag gtaggccatg cccggttctg tcaccatcag ccatcacgag tcggcggtcg
     6001 ccctggatca cgcggacgcc aagcggctcg ccacggtgct ggaggagctc gcgtacctgc
     6061 tggagatccc cggtccgaac cggatcaacg acgcgcagct cggcgccctc tgcgagggcc
     6121 ggtcgccgga ccgggccgag ttgtcgcact ggtcgcgcgg gatcgccgcg gagctgaagg
     6181 gacggctcta gccgcggccg tggcggaccg gccggagtcg tacgatggcg cgcggggcgt
     6241 gtggttctcg cgcgccttcc gggtgccggt ccgaccggcg gccggaccgg cggaggaggg
     6301 tgggggcccg cacgtgccgc agtccgaggc gcattccgag ccgcagtccc ggcggacgtc
     6361 agacccgcag cccggccgga ggacaggccc gcagccgggc ccgatgtcct ggctgaccgt
     6421 ggagacgccg ttgcccagcg ggcccatgca catcgcggtg acgccgctgg gcgtggcggc
     6481 cgcgggctac ctggggcacg cgggcggcga actgccccac tgcacggacg agtcgaagat
     6541 cgccgccgtc accgaccgga tcggagccta cctggcgggc cgcagccggg agttgggcct
     6601 gccgatcgac tggagcctga gcaccgggcc gcaccgcgcg gtgttgcaga cgctctggcg
     6661 cgacgtgccg ttcgggcgga ccatcacgta cggcgaactg gccgcccgta gcggggtgtt
     6721 cgagtccgtg acggagccgg ggctggcggc ccggacggtc gggcagatga tggccgccaa
     6781 ccccgtcgcg ctgctggtgc cgtgccaccg ggtggtggcg gcggacggga tcggcggatt
     6841 cggcggcggc cgggtcggga tcgacgtgaa gcgctggctg ctcaccctgg agggtgtgtt
     6901 ggagccgacg ctggactgga acgggccgct gtgaccggcg gaccgggccg ccggagcggg
     6961 cccggccggc cgcgccgctc agtgcaccag ctccaccggg gtgaggccgc ggccgaacgg
     7021 ggactgccgc ggcaggaccg gccggacgcc cgagaccagc gtgtagtcga tgcccgtgtg
     7081 gtcgatgccg gcgagttccc cggcgcgatc ggtgtcgggc cgctcgccga gcgccaactg
     7141 caccgcgttc cacggcaggt tgaggccgca cagacgcagc tggtgcaggc cgcccgaggg
     7201 ccgggtgttg acgtcgatga tcaccggttc cccggccagg tggcggaact gcacgttgga
     7261 gaggaaggcc atccgggtgg actcgaccac ccggcgggcg ggttccaggt actgcggggc
     7321 ggtggtgaag cttcggcggc gccggtgctt gctgcggccg acggcggcga gcagggtgcc
     7381 gtcgcggtcg gcgaggcagt ccaccgagac ctcggggccc tccaggtagg gcatcaccag
     7441 caggtcggcg gctccggcag ggccctcggg cgcggcggcc tccagtgcgg cggccacctg
     7501 gtcgacctgg acccggctgt cccagccggc cagccggtgg aggccgaagg gctcgcgggt
     7561 gagcacccgg aacccctcgc cgccggcgcc cgaggagggt ttgaggcagg gggtgagccc
     7621 ggcggcctcg atcagctcga cggcggcgat caggtcggcg gcggtgcgga cctgccacca
     7681 cggcggggtg ggcagtccgg cggcggcgat cgccgcgtag ccgtccgcct tgctgtcgaa
     7741 ggccgcgatg gcggtggcgg gcgggcagac cagggcggtg cctatcgcct cgaactcctg
     7801 ctggtgcagg gatatcgcga gctggtggag gcggggcagg aagacgtcga cggagtgccg
     7861 ggcgcagtag tcgagggcgt agccgacgta ggcgtcgggg gtgaggtcgc ccggctccag
     7921 ggtcgcgtgg tcggcggcgg cgaggacggg ggagtcgggg tcgacgtggg tcgcgtggac
     7981 ctcgaccggc cgcggggcct cgcgcagctg ttcgatgaag aacacgttct cggcgtaggt
     8041 gcggttgagc cagacacgta cgggggcgct catgacggcc tcccagggca ggggtgaacg
     8101 agggggaggg catccggtcc ggccatactc gtcaactggc cccgtggccc tttgaggtgg
     8161 caccttagcg gccgcccgga ccggcgtgaa cggagggtcg gccggggcgg tcggggcgtg
     8221 ggccgggccc ggggcggagg cgcggggcgg ctgccggtgc gcggtcgggc ggatggccgg
     8281 gcgggtggcc gggtgtgcgg cccggcggcg gcccggtcgg ggcattgtcg aatgttcgaa
     8341 taatggtcga acttgtatcg atcaggtgat ctagtcgtcc tatttgttcg gttattccga
     8401 ccactcgaaa gagtgattgc ggagcccgcc gaataggctc accatgattg ttgcaaggcg
     8461 acacttcacc gggtattcga agggctctcc atgtcgaccg aaaccaacgt cggtattcct
     8521 gcgccgggcg gcgagtcgcc tgcccagcag agtctcggca cggcgggcgc gaggaacctc
     8581 gcgacgacca ccaagtccgc gccgcagatg cagggcatca gcccgcgctg gctgctcagg
     8641 atgcttccgt gggtcgaggt gtccggcggc acctaccggg tcaaccgtcg cctgcggtac
     8701 gcggtcggcc gcggccgggt gggcttcgtg aagacgggct ccgaggtgcg gatcgtcccg
     8761 ccgacgctgc gcgaggtgcc ggtgctgcgc ggtttcgagg acgacgccgt cctggaggag
     8821 ctggccgggc gcttcgtcca acgcgagttc tccgccggtg aggtgctggt cgccgccggg
     8881 gcgccgatcg acgaggtctt catcattgcc cacggcaagc tcgaccggct gggatccggc
     8941 aagtacggcg acgagacggt cgtcggcagt ctcgccgacg gcgaccacct gggcgacgag
     9001 gcgctccggc aggaggacgg cgcctggccg tacacggtga gggccgccac cgcaggcacc
     9061 gtcctggcga tctcatggcc ctcgttccag gagctcgcgg accgctccga ggcgctccag
     9121 ctccagatca tggactacct ggccggcttc gagcaggcgc agaacaagca cggcgaggcg
     9181 gcgatcggcc tctccgccgg ccaccggggc gagtacgagc tgcccggcgc cttcgtggac
     9241 tacgagctga agccccgcga gtacgagctc agcgtcgcgc agactgtgct caaggtgcac
     9301 agccgggttg ccgacctcta caaccagccg atgaaccaga ccgagcacca gctccggctg
     9361 accatcgagg cgctgcgcga gcgccaggag gacgagctcg tcaacaaccc cgagttcgga
     9421 ctgctgcaga acgccgagta cgaccagcgg atccagaccc actccggccc gcccaccccg
     9481 gacgacatgg acgagctgct gagccgccgc cgctccacca agatgttcct cgcccacccc
     9541 cgggcgatcg cggccttcgg ccgggagtgc agccgccgcg gcctctaccc ggacaccgtc
     9601 gaggtccagg ggaggcaggt cttcggctgg cggggggtgc cggtcttccc gtgcaacaag
     9661 atcccgatcg tcggcggtaa caccagctcg atcatcgcgc tgcgcaccgg ggaggacaac
     9721 cagggcgtca tcgggctgca ccagaccgga attcccgatg aataccagcc gggcctctcg
     9781 gtccggttca tgggaatcag cgagaaggcc atcatttcct atctcgtcag cgcctactac
     9841 tccgccgcca ttctggtgcc cgacgcggtc ggagtcctgg agaacgtcga cgtcgcgcac
     9901 caccgcgact gaccgggccg gtcgcgaccg cgccgcccgg ccggggctta cttgcgcccc
     9961 ggtcgggcgg cgcccaccgt cacaacaggg ggaggaaccc atgggaatct tcacgctgga
    10021 acgcgaccac cgcgaggggc acgaggcgga ggacgtgctg gcccgcgccc gcggcctggt
    10081 cgacccggtg atgcgcgccg ccgtcgactc gctgcccggc tcgatgcgca aggtggccgg
    10141 ctaccacctc ggctggtggg aggcggacgg cacgccggtg gccgcgccct ccggcaaggc
    10201 catcagaccg gcgatggtgc tggccgccgc ccaggccctc gggggcaccc cggccgcggc
    10261 caccgccgcc gcggccgccg tcgagctggt ccacaacttc accctgctcc atgacgacgt
    10321 catcgaccgt gacgagacca ggcgccaccg ccccacggcc tggcgggtgt tcggcaccac
    10381 cgaggccatc ctcgtggggg acgcactgca ctccctggcc ctgcggctgc tcgccgggga
    10441 cacccacccg gccgcccagg gcgccatccg ccggctgacc gactgcgtgg tcgagctctg
    10501 cgagggccag cagatcgact gtgccttcga gcagcgcagt gacgtttcgc tcgccgagtg
    10561 cctggcgatg gcggaggcca agacgggcgc gctgctcggc gccgcctgtg cgatgggtgc
    10621 gcagtacgcg ggcgcctcgg aggaggccgc gcaggcgatg gacggcttcg gccgcgagat
    10681 cggcctggcg ttccagctga tcgacgacct gatcgggatc tggggcgacc cggccgtcac
    10741 cggcaagccg gtcggggcgg acttggtcgc gcgcaagaag tcgctgccgg tggtggcggc
    10801 gctcggctcc ggcacggccg acgggcacgt cctggccggg ctctatgcgc tcgaacgccc
    10861 tctcagcccg gaggagttga accgctgtgc ggccgccgtc gacggggccg gcgggcgggg
    10921 ctgggcccag ggggagtcct gcgagcggat ggccaccgcg atggcccagc tggccgtggc
    10981 ggtgcccgag ccgtccgccg ccgacgagct gctcgccctc gcggagctgg tcacccgccg
    11041 cacgcactga caccgcgggg cgggcggggg ctggtcggtc cggctcctgc ccccgcccac
    11101 gcatgccccg gaggggctct gccggcgtcg gcgagggtta ctcggcgtcg gtcgttccca
    11161 gggccgcgag caggcgctgc tccttcgccg ggacgatccc ctggaggtgg tgagcgccct
    11221 tgcggccctt gaggccgtcg gcattggtgt cctcggcggg ctcgcgctgc tggagccagg
    11281 cccaggtgtc gcgcgcggtc tcggcgaacg gccggcagcg gagcccggcc tcctgcgccc
    11341 gggcggtgtc ggcgagccag acggccgcca tctccggtat gtccggtgcc cagagcggga
    11401 gttccagcca cggctggacg ctgtccgggc cctcggccag caacgggccg tcctccaccc
    11461 agaccagctc ggcgcgcgga cggccgtcgg agggctcgcc ggccacttcc cggcaggtgg
    11521 ccaggaaccc cccgaaggtg acgtttccgg ccggggcggt ggtgaggtag cgcccggtgt
    11581 gcccggcggt caccaccccg atgccgaagg cggcgaagtc tcggacgtcg atcacctgca
    11641 tcgcgcgctc cgggtcgccc ggcgccagca ccgggccgcc gcgggccatc cgctccagcc
    11701 accagggcag gcggccgccg gtctcgtacg ggccgatcag cagcccgctg ttgaggatca
    11761 gcgtgcgtcc ggcgccgaag tgttccagca ccgcgcgctc gcagccggcc ttcagcgcat
    11821 tgctgaacgg ctggtcggcc ggggtgtcgg cggggcaggg gtgcagcggc gactcctcgt
    11881 tcaccacctg gcccggccag tcggcgaagg cgtgcacgga ggagacgaag gtgtagcggt
    11941 cggcgtgctc gcgcagcagc cgggcggaga tcacggtctg ggcgggctgc tgcgccgagg
    12001 tgtcgacgac ggcgtcccag tgccgcccct cgaccagctg cgtcaggtcg tcggcggagt
    12061 cccgctcgcc ccggatggcc tcgaccccgg ggaggtcggc ataggagcga ccgcgagtga
    12121 aggtggtcac ctcgtggccc cgggcgaggg cctcggaggc gaaggcccgt ccgaggaagg
    12181 acgaaccgcc aagcaggagg attctcatac cggcaaggct gcctccaccc ggccgtccgc
    12241 atgcgcccgt tcgcttccag cggaggcagg cctgcgacgg gtaccggagc ccgtcgaggt
    12301 gctgatccaa gtgccgaggt tgatctgccc gtcgaggcgc tgatctgccc gccgaggtgc
    12361 tggtccgccc gtcgaggtgc tgatccaatc gggcccgcat agcatccccg tgtaccgata
    12421 tgtctgattt aagaacgatg cttcggcact ggggagagcg acagcatggt caacccggca
    12481 ctcgtggacc cggccaccat ccgcgcgacc gccgccgagg catgggtccg cggctatccg
    12541 atcctgcaga actaccgcac cctgtacgcg caggccgtgg acgacgcgga cccgaggtac
    12601 gtcgggggct tcggtgtctt ccggcactat ccgcagccct tcaccccggc caacaccgac
    12661 atcgtcaccc ccaacaacga caccccctac tcgtgggcct ggctcgacct gcgggccgag
    12721 ccgtgggtgg tctccgtccc cgcctcggac cgctactacg tcgtccccct gcacgaactc
    12781 gacaccgtgt acgccgggtt catcggcacc cgggtcaccg gccaggaggc cggcgactac
    12841 ctcgtggcgg gccccgactg gcagggcgag gcacccgagg ggatcaaggg cgtcatccgc
    12901 accgccaccc ggctgatcgg catcgtcggc cgcacctacc tcgccgggac ctcacccgag
    12961 gacgtggagg agctggagcg gatccagcgg cagtaccagc tgcgcccgct gcacgagtac
    13021 gccggcaccc cggcgccgcc gccggccccg cacccggtct ggccggtctg gcgcgaggag
    13081 gtgatggact ccatcgagtt cctcggcttc ctcgacttcc tgctcgggtt cttcccggcc
    13141 ccgccggccg aggccgacct gcgcgggcgc ctcaccgacc tcggcgtcga cggccgcggc
    13201 gagttcgagc ccgccgccct gccgctggag gtccgcgccg agatcgagcg cggcatcgcc
    13261 gacggccgcg ccgagctcga ccgcgccgcc cgcgagacct ccggctccac cggcctcttc
    13321 ggcacccgcg agcaggtcgg caccgcgtac ctgaagcagg cgctcggcgc gatggagggc
    13381 ctgtacgggc tgcccgtcga ggaggcctgg tacagcggct ggctcgccga cagcgagggc
    13441 ggccggccac cgaacgccag caaccacgac tacatgctcc ggttcgcccc ggaccggctg
    13501 ccgcccgcac ggttcttctg gtcggtcacc atgtacgccc tgccggagcg tctgctggtc
    13561 gacaacacgc tggaccggta ctcgatcggc gaccggaccc ccggactggt ccgcgacgcg
    13621 gacggcggcc tcacgctcta cgtccagcac aagcggccag ccgacgccca gcactcggcc
    13681 aactggctgc ccgcgccgga cggcccgttc accgtggtca tccggctcta cggcccggac
    13741 gagtcggtcc tcgacggccg ctggacgctc ccgccgctga cgccgcgctg acgccacgcc
    13801 gatcgggcgc cccaccccca ctccgcgccg cccgcccgcg ccgcccgtcc gcccggacct
    13861 gcgttccacg tgcccgcccg tggggacccg tgccacccgt ccgcccaccc gtagccatgc
    13921 cggacccgag aaggactccc gatgacccag cccgagctgg aggccctggc cgccgacgcc
    13981 tacgtctacg gctaccccct ggtctccgac ctcaccatgg tcgagcgctt caccgagggc
    14041 ggcatgggca tcctgccggc ggctccgttc aaccgggtca gccatgccgg gcggctggtc
    14101 ggaccggacg acccgttcgt ctccgtcaac aacgacaccg tctactcggt cgcccagctc
    14161 gacctctccg agggcccgca ggtgctgcac gtgccggaca ccgacggggc gtactacgtc
    14221 ctgcagttcg tcgacgcgtg gaccgacaac ttcgcctacg tcggccgccg ggccaccggt
    14281 accggcgcgc agacctggtt gatcgtcccg cccggctggc acggcacccc cacggaccac
    14341 gagcacgtga tcgtctcgcc gaccacggtg gccagcatcg tcggccgctt cgcctgcacc
    14401 ggccccgagg acctcccgcg cgtccgcgcc ctccaggaag ggctgacgct cgaaccgctg
    14461 gagccgggcg ggctcgccgc cgggctgccc ggaccggacc cggaggtgcc cgagcggctg
    14521 gcgttcttcg aacggctccg ggtctggatg gcggccttcc cgccggccgc cccggacgtc
    14581 gactaccagc agcggttcgc cccgctcggc ttgctcgaca ccgggccctc gccgtaccgg
    14641 gccgccgcgc ccgagtggac gctcgccctg ctcaagggcc tggctgccgg ccgcgaacgg
    14701 gtcgaggacg ccatccggcc gccggaggac cacccggccg gcgagtggcg gaccgccctg
    14761 cacctgttcg actacaacat cgacttcctc ggccccggca cgatcgacga gcccgactgg
    14821 cgcatccccg accgccgcgc cgcctacctc agccgggccg ccgccgcccg cgccggcctc
    14881 tggggcaacc acgcgtacga ggcggtgtac gcgatgacct ccgacgacgc ggacgggcac
    14941 ccgctcaacg gcgcccacac gtacacgctg cgcttcgacc agcccccgcc ggccgacgcc
    15001 ttctggtccg tcaccatgta cggcctgccc gagcactacc tcgtggcgaa cccggtcggg
    15061 cggtactcga tcggcgaccg cacccccggc ctggtccacg gcgaggacgg gtcgctgacg
    15121 ctccggctcc agcacgagca gcccgaggac ccggccgggg cggcgaactg gctgcccgtc
    15181 ccggagggcg ggttccgccc gatcatccgg ctctaccagc cggggccgac cgtcctggac
    15241 ggcacctacc gggtgccgcc gatccggcgg agctgagccg caccggcacg ggagccgacg
    15301 accgggcacg acctgacggc cgcgcccgac ctgaccgcca cgtacgaccg acgacccgcg
    15361 caaccgagga gcacgtacga ccgacgccca cgtacgaccc gacgaccacg cgtaggcgag
    15421 ggccgggcac ggagccggcg agctgccgtc gtaccgacga agggatgcag ggatgggcga
    15481 cgccgtcggc cagatgctgg tctcggccat gggcatcgcg atcagcccga tgccgttgat
    15541 cgcgatgatc ctgatgctgg ccaccccgaa ggggaaggcg aacggcctgg ccttcacggc
    15601 cggttgggtg gtggcgctgg cggccgtggt cggcgtggtc gtggcggccg gttcggggct
    15661 gagcgccgga tcccccaagc cggcctgggc ctggtggctc aagctggcgc tgggtgtgct
    15721 gttcctgctg ctgggcctca ggcagtggcg ggaccgcccg cgtgaggggc acgtgcacgg
    15781 cccgccgaag tggatgcagg cgatcgaccg gttcaccccg cgcaaggcgg ccggtctggc
    15841 ggccgtcctg gtcgcggcca acccgaagaa cctggtgctg gcggtcggcg gcgccgtctc
    15901 catcgcgacc agtacggcct ccggcggcgg caaggcggtc gcggccgcgc tgatggtgct
    15961 gatcggctcg ctctgcacgc tgctgccgct gggggtctac ctgttcggcg gggaccggtc
    16021 ggcccgggtg ctgggggagt ggaaggcgtg gatggcggcg cacaacgcgg cgatcatggt
    16081 cgtggtcctg atcgtcctcg gcgcgaagta caccggcgac gcgatctcgg gcctgacgac
    16141 gtagggccgt ccgtgcgccg ccgcgcccga ccggcgcagc ccgccgtgtc caccccgccc
    16201 atcctgccag ccgtgccccg accggctcac cgcggcgacg cggacccggt cgccgcaggg
    16261 gcggacgcac ccccggacga cgacggcggc ggcgacgacg gcgaggccga cgccgtgccg
    16321 gtcggcgcgg gtgtgcgggt cggcgcgggc gacgagggag ctcccgtcgg cgaggccgtg
    16381 gtgccggggg agggcggcgg tgagccgggc gcggtgctcg gcccgccgga cggagtcccg
    16441 cccggcgacg ccggtcccga cgtcggtccc gccgtcggtc ccgacgtcgg tcccgacgtc
    16501 ggtcccgacg tcggtcccgc cgtcggctcg gccggggccg aggcggagcc gccccggtgg
    16561 tgcgggccgc cctccagggt caccacggcc tgtgagggcg gcagcgcgat cctcgcggtc
    16621 cagtgcgtgc ccggggcgag gtcctcgtcg acggtgacga tcacggtgat ccgctggccc
    16681 ggggcgagcg ttccggcttc ccggctgagc cggagccagg cggcgtccac ctcggcgtgc
    16741 cagcggatct cggtgacccc ggagttggtg agggtgagaa cggtccggtt cccgtactcg
    16801 ccggcctcga cggtcagcag cccggccgga gcgggcccgg gggctcccgg cccgccgcct
    16861 ccggggccgg ccgccgcacc ggccacgggt gccggtgcgg cgaccagcgt cggactgccg
    16921 agcggagtcg cgccgtgcgc cggaaccggg atggccggcc cctgaatccc cggaagcaac
    16981 gtttctgcac ggagcggtcc ggccccggca agctccagcc cggccaccgc gggagccgcc
    17041 ccgacggcac ccaccacccc gccgacctgc ccgacctgcc cggcctgacc gggctccccg
    17101 ccggcgcccc cggacccgcc cggccggcgg ccggcggccc gctggtcgtc ctcgacccgg
    17161 acggaggaga ccgccgcggc agccccggcc ccgccgccac cccggtggcc ggcccagagc
    17221 gcgaccaccg gggccgccag caccgcagcc agcacgcccg tggtcaccac ccgccggcgc
    17281 accgccggtc cgcggcgcgc gtccggcgcg cggtgacggg ggaacccgcc ctggtcgaac
    17341 cgcaggccgg gctcgaccac cggggccggg accgccgtcc gccgcgccgc cggggccccg
    17401 gtcgcgcgcg gcaggaacag caccccgccg tgccggacgg ccccgcccgc cggcgctgcc
    17461 gtccgtacgg cgggagcgga cggcgcggtg agcacgggca ggcccgagag gcccgtcgtc
    17521 ccctccgtta cctcacccgc cacccgctcg gtggtgcccc ggcaggtcgg gcagtccacc
    17581 acgtgctgca ccagctcgcg gcgcagtgcc gggccgagta cccagtcccg ccagctctcc
    17641 gcgccggccc cgccgagccg gtccagctcg gggcagctgc cgacgccgag caccagcagc
    17701 gccgtcctgg tccggccgac ctccgcctcg gccgaggcga gcagcgcacg ggacgtctcg
    17761 aacgggacgc ccagcacggc cgccacctcg atcggggtga gccggtggcg gaccgacagc
    17821 tccagggcct cgcgctgctc ggggtcggtg ccggccgcct cgggccaggc gagcgaggcc
    17881 agctcgcgcc gccgcctggc agcctcggcc gtcggcacgc ccgcccccgc gcccgagggg
    17941 ccggagacga cgcccggcga agcggagacg cccggcgaac cggggacgcc cggcgaaccg
    18001 gagaccacgc ctgtcgggcc ggagagcacg cccgtaggac tggagaccaa gcccgtagga
    18061 ccggcgccgg ccccgggaag cccgccaccg gaccccgcgc cgtcccccat ccgccgcagg
    18121 caggagtacc gcgccagcga gtacagccag gcccgcacca tcccgggctc cgccagccgc
    18181 gtcccgtgcc gcagcgccag ctcgcgcacc tcgcgcaggg cggccgcggc cgcgtcgtgc
    18241 tcgcacagca ccgagaggca gtaggtgaac agaccgtcca cctgccgctc gtacgccgcg
    18301 agcacggggt cgcgcggccc ttcagtactc gttgtcaccc gcccgacggt agagagacgg
    18361 ggcgccgagt ccgcgccatt ccctgctgtt gtacttcttt cgggtaacaa catcgcccat
    18421 tcgtggacct tccacgagcc cccgcgtccg gtcccgcgcc ccctgcccgc cccggtccgc
    18481 gccccctgcc cacccggccc gcggcccgtt gtcgtaccca ggcgctacgg tgaccgcatg
    18541 gctgcccgca ccaagaccac cgccaagccg cgcccggcgt accgctgcac cgagtgcggc
    18601 aaccagctgc ccaaatgggt cggccggtgc ccggagtgca acgcctgggg cacggtcgag
    18661 gagtacggcg ccgtcccgat ccggacgacg gccgccggcc cggtcagcgc gcccgcccgc
    18721 ccgatcgccc aggtggacgg tcaggtggcg acggcccgct ccaccggcgt gcccgagctg
    18781 gaccgggtgc tcggcggcgg cctggtcccg ggcgccgtgg tgctgctggc cggtgagccg
    18841 ggtgtcggca agtccaccct gctgctggac gtcgcggcca aggcggcctc cgcccagcac
    18901 cgcacgctct acgtcaccgg tgaggagtcg gccggccagg tccggttgcg cgcggaccgg
    18961 atcaacgccc tctccgacca cctctacctc gccgccgagt ccgacctcgg cgccgtcctc
    19021 gggcacatcg aggcggtcgg tcccggcctg ctgatcctgg actcggtgca gaccatcgcc
    19081 tccgcggagc tggacggcgc ccccggcggc ccggcgcagg tccgcgaggt ggcgggcgcg
    19141 ctgatccggg cgtccaagga gcgcggcatg gccaccctgc tggtcggcca cgtcaccaag
    19201 gacggccaga tcgccggccc ccgcctgctg gagcacctgg tggacgtggt gctcagcttc
    19261 gagggggacc ggcacgcccg gctgcgcctc atccgcgggg tcaagaaccg ctacggcgcc
    19321 accgacgaag tgggctgctt cgagctccac gacgagggca tcgtcggcct ggccgacccg
    19381 tccggcctct tcctgaccag gcgtgacaag cccgtcccgg gcacctgcct caccgtcacc
    19441 ctggagggca agcgcccgtt ggtggccgag gtgcaggcgc tgatggtgga ttcgcagatc
    19501 ccgtccccgc gccggacgac ctcgggcctg gagtcgcccc ggatcgcgat gatcctggcc
    19561 gtggtcgagc ggcacggcgg ggtcaagctc ggcaagcagg acatctacac cgcgacggtg
    19621 ggcggggtga agctgagcga gccgtccgcc gacctcgcgg tcgcgctcgc ggtggccagc
    19681 tcctccaccg acaccccgct cccgagcaac ctggtggcca tcggcgaggt cggcctggcg
    19741 ggcgaggtgc ggcgggtgac gggtgtgcag cggcgcctgg ccgaggcgca ccggctgggc
    19801 ttcacccacg ccctggtccc gccggacccg ggcaaggtgc cgccggggat gaaggtggtc
    19861 gaggtcgcgg acatcggcga ggcgctgcgg gccatcccgg gccgccgccg ggccgccgcc
    19921 aagccgcgcg gtgaggcacc gtcggcgccg cccactccgc gccgggccgc ctccgcaccc
    19981 cccgtcgcgg cccacccgga ggagctgatg gagggctggg agccgctcga ctcggacgaa
    20041 ctgcgctgac ccaagccctg tcgttgccgc acgcagttag actttcccct gtctcattgg
    20101 tgataaggca gtactaagcg acagagcaac agaccgtagg ccggcgcacg gcgcgaggcg
    20161 cgcggtacgg ggtcgaccgg aggagtcacg tggcagccag cgaccgggcg gacaagtcct
    20221 cccgcgagga ggccctgctg cgggcctccc tcaccgccat cgcgcccggc acggggctgc
    20281 gtgacggcct ggaacgcgtg ctgcgggcca acaccggtgg tctcatcgtg ctcggattcg
    20341 acaagacggt cgagtcgatc tgcaccggtg ggttcgtcct ggacgtcgag ttcaccgcca
    20401 ccaggctgcg cgagctgtgc aaactcgacg gcgccgtggt gctggacaag gacatcacca
    20461 agatcgtccg ggccggggtg cacctgatgc cggactcgac catccccacc gatgagaccg
    20521 gcacccggca ccgcaccgcg gagcgggtca accggcagac cgggttcccg gtggtcgcgg
    20581 tctcgcactc gatgcggctg atcgcgatgt acgtcaacgg cacccgccgg gtcctggagg
    20641 actccaccac cgtcctctcc cgggccaacc aggcgctggc caccctggag cggtacaagc
    20701 tccgcctgga cgaggtcgcc ggcacgctct ccgccctgga gatcgaggac ctggtgacgg
    20761 tccgcgacgt caccgccgtc gcccagcgcc tggagatggt ccggctgatt gccaccgaga
    20821 tcgccggcta cgtgctggag ctcggcatcg acggccggct gctctcgctc cagctggacg
    20881 agctgatcgc gggcgtggag ccggagcgcg agctggtcgc gcgggactac ttcccggagc
    20941 gggccgccaa gaagggccgg acggtcaccg aggtgctggc cgacctggag gcgctgaccc
    21001 acgccgagct gctcgacctc cagacggtgg cgaaggcact cggctactcg
//
