LOCUS       NZ_CP026498            11358 bp    DNA     linear   CON 22-MAY-2024
DEFINITION  Streptomyces sp. CB01881 chromosome.
ACCESSION   NZ_CP026498
VERSION     NZ_CP026498.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. CB01881
  ORGANISM  Streptomyces sp. CB01881
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 11344947)
  AUTHORS   Liang,H.
  TITLE     A 3-hydroxy-3-methylglutaryl-CoA synthase gene based probe for the
            discovery of the acyltransferase-less polyketide synthases
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11344947)
  AUTHORS   Liang,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2018) Xiangya International Academy of
            Translational Medicine, Tongzipo, Changsha, Hunan 410000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002953255.1-RS_2024_05_22
            Annotation Date                   :: 05/22/2024 02:39:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,978
            CDSs (total)                      :: 7,904
            Genes (coding)                    :: 7,565
            CDSs (with protein)               :: 7,565
            Genes (RNA)                       :: 74
            rRNAs                             :: 5 (5S)
            complete rRNAs                    :: 5 (5S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 339
            CDSs (without protein)            :: 339
            Pseudo Genes (ambiguous residues) :: 0 of 339
            Pseudo Genes (frameshifted)       :: 124 of 339
            Pseudo Genes (incomplete)         :: 251 of 339
            Pseudo Genes (internal stop)      :: 10 of 339
            Pseudo Genes (multiple problems)  :: 45 of 339
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:44:32
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4945559
            Orig. end                         :: 4956917
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP026498.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11358
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="17"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11358
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11358
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6358](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6358
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(1588..2613)
                     /locus_tag="C2142_RS11560"
                     /old_locus_tag="C2142_11545"
     CDS             complement(1588..2613)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018105824.1"
                     /locus_tag="C2142_RS11560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C2142_11545"
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="WP_148642560.1"
                     /transl_table=11
                     /translation="MTISRANVSAIAEDLYVWLPPKRGWGLANCGLLASPGTALWIDTP
                     YDPSLAAEFLAESRQRLADGVTVDRIVVTHANGDHLWGAGVLPDAEVIATREALGHIHW
                     EPSPKQQHALVTGSDPASLLGGYLQRHFGQFDWSETEPVHPQTVFSGELELRVGEYPVQ
                     ITALPSAHTGGDLIVHLPHQRAVFTGDVIFGSTPEQPGDHPSHWAGPLENVIGACEQAL
                     ATGAEIFVPGHGPVLDRSGVRDHIAYLGYVRERAHALHEAGIPALDAARRIIAEQRHPE
                     LGLPERLVLTVAAEYRHLDGSEVPNVLEAMSQVALVARELDEDAGRPSRPRSGQDAPAS
                     A"
     misc_feature    2604..2624
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 19.75"
                     /tool="antismash"
     gene            complement(2880..3626)
                     /locus_tag="C2142_RS11565"
                     /old_locus_tag="C2142_11550"
     CDS             complement(2880..3626)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="C2142_RS11565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="C2142_11550"
                     /product="hypothetical protein"
                     /protein_id="WP_148642561.1"
                     /transl_table=11
                     /translation="MEILLQVLVGFVPAVIGFLAARSLARARLFLQYGFLRALLAPYSR
                     VQIVTPGIEVAEFAFAKDGRRLTSRGPRNVLFMPMAEARAIAQLIDLLRKVNRKATIEL
                     VAAGSQDPRMPTFSIGGPSVNRYSASVLGAAFPDFCIEYPEAARARWGGMTFEAQYNAA
                     DELIRDHGFVFVTRTSRQAPCIVVCGVFAFGSAMAVEALAATGRKSPLAALFRSGEKGF
                     AAVEGRVIGLDTVDVTVEVCRILPAV"
     gene            complement(3820..4743)
                     /locus_tag="C2142_RS11570"
                     /old_locus_tag="C2142_11555"
     CDS             complement(3820..4743)
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /locus_tag="C2142_RS11570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C2142_11555"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_148642562.1"
                     /transl_table=11
                     /translation="MTTLTIRDFRPADAGAVAAAHSAGREHLVLTPEVVVWMDANLPAG
                     RHHRTLVAELDGRVVGALRCGLVTGTTTRGVGYANGSVLPAYRRRGAFTALLAEAEAYL
                     AGHGVVELHSWVDEEPGALEFAANRGFIAGRTAHFARRDLSLPLPAAPALPAGVELRPA
                     SEWVEDPYPIFVVEADAIRDEPGEVAMDAADYEEFLHGDWARPDLDRELTTVAVLDGEP
                     VAFSAVQTDGADRYWSAFSACRRDFRGRGLTKLAKLESLRRAQVAGYRRAYTSNDATNA
                     PMLAINEWLGYERCASERKFVKRLDG"
     misc_feature    complement(4854..4867)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 12.28"
                     /tool="antismash"
     misc_feature    complement(4855..4864)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.63"
                     /tool="antismash"
     gene            5001..6358
                     /locus_tag="C2142_RS11575"
                     /old_locus_tag="C2142_11560"
                     /pseudo=""
     CDS             5001..6358
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944755.1"
                     /locus_tag="C2142_RS11575"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="C2142_11560"
                     /product="DUF692 domain-containing protein"
                     /pseudo=""
                     /sec_met_domain="DUF692 (E-value: 4.2e-90, bitscore: 300.5,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSPALRAPVKGTAPDRATAAPAPATATPAQAGTLGVGLGWRSEI
                     DIVVERQPGLDWVEVVAENVCAGHLPQSLRILRERGVTVVPHGVSLGLGGADRPDPARL
                     DRLAETAGALGSPLVTEHLAFVRAGGLEAGHLLPVPRSRDALAVIAENVRIAQDQLPVP
                     LALENIAAQLAWPDDELTEGQFLAELVERTGVRLLIDVANLHTNRVNLGVDPAAELDRL
                     PLEALAYVHVAGGRLVDGVWHDTHAHPVTEPVLEVLAELCARVAPPGVLLERDANFPPP
                     GELAAELDAVRRVLEGARRS"
     gene            6399..7451
                     /locus_tag="C2142_RS11585"
                     /old_locus_tag="C2142_11565"
     CDS             6399..7451
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="C2142_RS11585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C2142_11565"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_148642563.1"
                     /transl_table=11
                     /translation="MWHNTYFLVPAVLLVAAALYAPQTKQAAQRVPQAKGLPGRGLPLL
                     DTAFLSGGPGRVFDTAFVRMHQSGRLVVNRDGLVTLTGGRPYDSVEEAIIEALGPAGQR
                     ELAPLRRAVMRSAAVQKLGDGLAHRGLLRNPVLLRRAKVARRPLWLALLVVVLSTVLAY
                     VLDNGEADRPALWIPAVLLIVGLASVIVSWPLKGRITPAGRRQLALMDTGKPWTPADAV
                     APLATGAALLGAIALGGLAAAGLEDAELQEAMLAAAAAEEAAARAAAGVSSSSASSSGS
                     SCSSPATWCGASTDSGSSGSGCGSSSGCGSSSSSCGSSSSSSCGSSSSSCGSSSSSSCG
                     SSCGGSSCGS"
     gene            7552..8331
                     /locus_tag="C2142_RS11590"
                     /old_locus_tag="C2142_11570"
     CDS             7552..8331
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014175677.1"
                     /locus_tag="C2142_RS11590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C2142_11570"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_148642564.1"
                     /transl_table=11
                     /translation="MWLLFLIPACAAAVLSCIRLVRMAATADALADQEGPRPPEAVGIG
                     LYETAYLAGGPDRVVELALVLMASRGRLHLAHTGWTTVVDPKGRSRLERAVIATVGPDG
                     QCRTDDLRRAMAAHPTVLEIGGRLALAGLATPAPVQQSTVLVIRQVRQALLLSLVLMAG
                     AVAVGGLSDGDALAPLAWFSLPLVLTAGTLLMARVDVYPYTRWASPIGQEVLREVRPPR
                     QHGLGDDDERELLTAVAMIGPAAVPDPRLRAALRHRH"
     gene            complement(8578..9303)
                     /gene="hemQ"
                     /locus_tag="C2142_RS11595"
                     /old_locus_tag="C2142_11575"
     CDS             complement(8578..9303)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138586.1"
                     /locus_tag="C2142_RS11595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C2142_11575"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_148642565.1"
                     /transl_table=11
                     /translation="MTETVEQQPEQQPEKKKARDLNQVIRYTMWSVFKLKGALPEDRTA
                     LAAEVDELFDQLAAKDVTVRGTYDVSGLRADADILVWWHAADSDDLQEAYNRFRRTGLG
                     RELEPVWSNMALHRPAEFNKSHIPAFLADERPRDYVCVYPFVRSYEWYLLPDAERRAML
                     AEHGQMARGYPDVRANTVASFALGDYEWLLAFEADELHRIVDLMRDLRPSRARLHVREE
                     VPFFTGRRKPVSELLAGLV"
     gene            complement(9300..10736)
                     /gene="hemG"
                     /locus_tag="C2142_RS11600"
                     /old_locus_tag="C2142_11580"
     CDS             complement(9300..10736)
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015579252.1"
                     /locus_tag="C2142_RS11600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C2142_11580"
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_148642566.1"
                     /sec_met_domain="DAO (E-value: 2.3e-09, bitscore: 36.1,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAQAQVVVIGGGIAGLAAAAFLSGAAGGPARARVTLLEASERLGG
                     KLSGGEVGGVRVDLGAESMLARRPEAVELARAVGLGAELEPPTTAKAAIWTRGALRPLP
                     GGQVMGVPGDLEALAKSGVLTAEGLERARHEGSGEGVADDVAIGRYVADRLGREVVDRL
                     VEPLLGGVYAGHADEISLRAAVPQLLAIARGGGSLVEGVHELATRPQTVSTPVFQGLRG
                     GIGTLPGAVAAACADAGVELRTRTAVEELRRTADGWRVVTGDGATLHADAVVLAVPAPA
                     AARLLRTDVPGAAAELDGVEYAGMALVTLAFRRADLPEPPAGSGFLVPPVDGRRIKAST
                     FSSNKWGWLERSAPDTFLLRTSLGRLHEEQALDLSDEELVAQSLADLGEAIGLTARPYD
                     TAVTRWRAGLPQYPVGHLDRVARIRAAAGRVGGLALCGAAYDGVGIPACIASARTAVDA
                     LDKVLTPLTGENVTERTMGA"
     misc_feature    10984..11000
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 23.62"
                     /tool="antismash"
ORIGIN
        1 gagcggctgg cgcctgcgcc tggtgggcag gccgctgccg tcggacggcg cggcgggccc
       61 cggcgtcacc tcgtgcaccg gcggcacgac gtgcatggcc ggcgcggcgt gccgggtcgg
      121 caggtggtgc ccgtccggcc cgtcgtaccc gttctggtca tcggactcgt cgtacggcgc
      181 cggcaccacc tgcacggtcg gcgcgggcgc cggacccgcc ccggcgcgca gcggcagcgg
      241 ggtgagcacc gagagcggct gctccgcgtc caccacggtc agcagcccgg ccgggatccg
      301 cacgatggtc cgcatgccgc cgtagggcga gatctcgatg tggcaggcga agccgtactg
      361 ccgggccagt ttgccggcca ccgcgaagcc ggtcttcgca gggttgccca gctcggtgag
      421 cagcacctcg ggcggaccgg ccagcagccg gcgggcacgg cccagctggt cctcctccat
      481 gccgatgccg ctgtcgtcga tgatcaccag cgcgccacgg ccgccctgct ggacggtgac
      541 cgggatgtcg gtctccgggt gcgagtacgc ggtggcgttg gcgagcagct cggcgatggt
      601 gatggcgagc ggctccgccg cccgggccac cacggccagc cggttctccc gcaggtggtt
      661 ggccaccttg atccgggcgt agccgggcac ccgggagagc gcgccgagca ccacctcggc
      721 cagcggcgag tcggtccggg ccagcccggg ccaggcctcg cagagcaccg cgatggtctg
      781 gatccggcgc agcgcgacct cgttgcggaa gtcgagttcc agcagccggg ggtcgtcgtt
      841 gtcgtgctgg agctgctgga ccaggctctg gatctggtag agcagcgtct ggatcttgga
      901 agtggcgccg cgcatcgcgg cgcgggcggc ggcgtcgacc cgttcgcgct cgtcggtgac
      961 ggcggtggtg gcggcgtcca ggacggcctg gagcagccgc acggcctcgg ggccgacggc
     1021 ctgcgggtcc agcgggccgt cggcgggagc gctggggtgg agaagccgca gtgaggcggc
     1081 actcacccgc tcggcggcga ggtggccgat ctcggccagc agggcgcggg cccgatcgtc
     1141 ggcctcggcg gtccggcgga cggcctcggc ggtgcggtcc tcggccgcgc gggcgagcag
     1201 ctcggcctcg gccgcacggt gctccgcctc ggcggtgcgt tgggcggcct cgcgggccct
     1261 gccctccgcg gccagcgccc gggtctcggt gcgctgcgcc tcctcggtgg cccggcgggt
     1321 gaccgcctgc gcctcgcgga gctgctgctc gccccgggcc agccggccgt cggcggcgcg
     1381 ggcccgttcc tcgccgtggg cggcggccgt ctcggcggcg acggcccgga cgagtgcgcg
     1441 ggtccgtaag cgggccagga caccggccgc cgcgagcgcg gcggctgcga gcactgccag
     1501 ggcgagcagc gcggtctgca tcatcgaacc tcggacgtcc tcgatcgaag ggggtggggg
     1561 tgcgccggag cgggcgcggg cgccgggtca ggccgacgcg ggggcgtcct gcccggaccg
     1621 gggccgcgac gggcggccgg cgtcctcgtc cagctccctg gccaccaggg cgacctggct
     1681 catggcctcc agcacgttcg gcacctcgct gccgtccagg tgccggtact cggcggcgac
     1741 ggtgagcacc agccgctcgg gcaggccgag ttcggggtgg cgctgctcgg cgatgatccg
     1801 ccgggcggcg tccagcgccg gtatcccggc ctcgtgcagg gcgtgcgcgc gctcgcggac
     1861 gtagccgagg taggcgatgt ggtcccggac tccggagcgg tccagcaccg ggccgtggcc
     1921 ggggacgaag atctcggcac cggtggccag ggcctgctcg caggcgccga tgacgttctc
     1981 cagcgggccg gcccagtgcg aggggtggtc gccgggctgc tcgggtgtcg agccgaagat
     2041 cacgtcgccg gtgaacaccg cgcgctggtg cggcaggtgg acgatcaggt cgccgccggt
     2101 gtgcgcggac ggcagtgcgg tgatctggac ggggtactcc cccacccgga gctccagctc
     2161 gccgctgaag acggtctgcg ggtggacggg ctcggtctcg gaccagtcga actggccgaa
     2221 gtggcgctgg aggtagccgc cgagcagcga ggccgggtcg ctgcccgtga cgagcgcgtg
     2281 ctgctgcttc gggctcggct cccagtggat gtggccgagc gcctcgcggg tggcgatcac
     2341 ctcggcgtcc gggagcacgc ccgcgcccca gaggtggtcg ccgttggcgt gggtgacgac
     2401 gatccggtcg acggtgacgc cgtcggccag ccgctgccgg ctctcggcga ggaactcggc
     2461 ggcgagggag gggtcgtagg gcgtgtcgat ccagagcgcg gtccccggcg aggccagcag
     2521 tccgcagttg gccagccccc agccgcgctt gggcggaagc cacacgtaca ggtcctcggc
     2581 tatcgcgctc acgttcgcac ggctgatcgt catatgcgcg attatgccga tgatgtgaca
     2641 ggtatgggag caaaagactt gtgattcaag ccatttcctg tcccggacca tcctgacctg
     2701 tcctacttcg caccggcacg gtccggccca tttcgtctca atgcacgggg acggacggac
     2761 cggtgaacgc gggaggtcgg accagccgac ttcccgttcc cgggcggacc cgaagcggcg
     2821 gatccacggc gcccggcacg cggatccacg gcggacggca cccggttccc gcccgcggct
     2881 cagacggcgg gcaggatccg gcagacctcc accgtgacgt ccaccgtgtc gagaccgatg
     2941 acccggccct cgacggcggc gaatcctttc tccccgctgc ggaacagcgc cgccagcggc
     3001 gacttccggc cggtggccgc cagcgcctcc accgccatcg ccgaaccgaa ggcgaagacc
     3061 ccgcagacca cgatgcaggg cgcctgccgg gaggtacggg tcacgaagac gaagccgtgg
     3121 tcccggatca gttcgtccgc cgcgttgtac tgcgcctcga aggtcatccc gccccaccgg
     3181 gcgcgggccg cctctgggta ctcgatgcag aagtccggga acgccgcgcc gagcacgctc
     3241 gccgagtacc ggttgacgct cgggccgccg atcgagaagg tcggcatcct cgggtcctgc
     3301 gagccggccg cgaccagctc tatcgtcgcc ttccggttga ccttccgcag caggtcgatg
     3361 agctgggcta tcgcccgcgc ctcggccatc ggcatgaaga ggacgttcct cgggccccgg
     3421 ctggtcagcc ggcggccgtc cttggcgaag gcgaactccg ccacctcgat gccgggggtg
     3481 acgatctgca cccggctgta cggcgcgagc agcgcacgga ggaagccgta ctgcagaaag
     3541 agccgggcgc gggcgagcga gcgggcggcg agaaaaccga tgaccgccgg aacgaacccg
     3601 accaggacct gaagcaggat ctccatagca tcccccgcgc ggaagactcg ggcacgaact
     3661 gtctgccgag aggtgatgcc ccgtagaaca ccgggaactc ccggcatcgc cgtccacccg
     3721 gcgcccggcg tcggcaatgc gcccacttca ccccttcgag tgaagccccc cgccccgcgc
     3781 ctggacggcc ggcggggcgg gcctcccccg gcccgcccgt cagccgtcca gccgcttgac
     3841 gaacttccgc tcgctcgcgc agcgctcgta cccgagccac tcgttgatgg cgagcatcgg
     3901 cgcgttggtc gcgtcgttgc tggtgtacgc ccgccggtag cccgccacct gcgcccggcg
     3961 cagcgactcc agcttggcga gcttggtcag gccccgcccc cggaagtccc gccggcaggc
     4021 cgagaacgcc gaccagtagc ggtccgcgcc gtccgtctgc accgcgctga acgcgaccgg
     4081 ctcgccgtcc aggacggcga ccgtcgtcag ctcgcggtcg aggtccggcc gggcccagtc
     4141 gccgtggagg aactcctcgt agtcggcggc gtccatcgcg acctcgcccg gctcgtcgcg
     4201 gatcgcgtcc gcctcgacga cgaagatcgg gtacgggtcc tccacccact cgctcgcggg
     4261 gcgcagctcg acaccggccg gcagtgcggg ggcggccggg agcggcagcg acaggtcgcg
     4321 gcgggcgaag tgggccgtcc ggccggcgat gaagccccgg ttcgccgcga actccagcgc
     4381 gcccggctcc tcgtccaccc agctgtgcag ctcgacgacg ccgtgcccgg ccaggtacgc
     4441 ctcggcctcg gccagcagcg ccgtgaacgc accccgccgc cggtacgccg gcaggacgct
     4501 gccgttggcg tagccgacgc ccctggtcgt cgtgccggtg accaggccgc agcgcagcgc
     4561 gccgaccacc cggccgtcga gctcggccac cagggtgcgg tggtgccgcc cggccgggag
     4621 gttggcgtcc atccagacga ccacctcggg ggtgagcacc aggtgctcgc gccccgcgct
     4681 gtgggcggcg gccacggccc cggcgtcggc cgggcggaag tcacggatcg tcagagtcgt
     4741 cataggtgcg ccacggtagc ccgccgcggc cccggttcac ctccgattta cccccccacc
     4801 tgcggttccg tggccgggag ggggttggag tcgctcgtca ccgggccggc aagctcaaat
     4861 aatgctctga gatgaccggg tgaggcttgg cagtcgccgc gtccggggac atcctcagca
     4921 acacagcccg gtcccgccct ggcgacgacg cccgcgccgg ccgacggctt cccacccagc
     4981 cctccgaagg gggcgcaccg atgacctccc ctgccctgag agcgcccgtg aagggcaccg
     5041 cacccgaccg ggccaccgcc gccccggccc ccgccacggc cacccccgcg caggccggca
     5101 cgctcggcgt cggcctcggc tggcgcagcg agatcgacat cgtggtcgag cgccagccgg
     5161 gcctggactg ggtcgaggtg gtcgcggaga acgtctgcgc cggccacctg ccgcagtcgc
     5221 tgcggatcct gcgggagcgc ggcgtcaccg tggtcccgca cggcgtctcg ctcggcctgg
     5281 gcggcgccga ccggcccgac ccggcccgcc tcgacaggct cgccgagacc gccggcgcgc
     5341 tcggctcgcc gctggtcacc gagcacctgg cgttcgtccg ggccggcggc ctggaggccg
     5401 ggcacctgct gccggtaccg cgctcgcgcg acgcgctcgc ggtgatcgcc gagaacgtcc
     5461 ggatcgcgca ggaccagctg ccggtgccgc tggcgctgga gaacatcgcc gcccagctgg
     5521 cctggccgga cgacgagctg accgaggggc agttcctcgc cgagctggtc gagcggaccg
     5581 gtgtccggct gctgatcgac gtcgccaacc tgcacaccaa ccgggtcaac ctcggcgtgg
     5641 acccggccgc cgagctggac cgcctgccgc tggaggcgct cgcctacgtc cacgtggcgg
     5701 gcgggcggct ggtcgacggc gtctggcacg acacgcacgc gcaccccgtc accgagccgg
     5761 tgctggaggt gctggccgag ctgtgcgcgc gggtcgcacc gcccggcgtc ctgctggagc
     5821 gggacgcgaa cttcccgccg cccggggagc tggccgccga actggacgcc gtccggcgcg
     5881 tactggaggg ggcccggcga tcgtgacgac accggaggac ggcgacctcg ccgccgcccg
     5941 cgcccggctg gccgagcgcc aggaggccct gctggccgcc ctggtggcgg gcgggccggt
     6001 cccgccgggc ttcgacgagg cccaggtcca cgcccaggca ctgggactgg cccacaagcg
     6061 cagggacacc acggccaagg tggccccgga gctgccccgg ctgctcgggc cgcagtacgg
     6121 gccgctgttc ctcgccttcg cgcgccggca cccgcagacc ggcgggtacc gcgcggacgc
     6181 ccgcgccttc gccgcatggg cgctgggcaa cacctcggcc tacggccccg gggaggggct
     6241 cggcccggac cagcggcagg ccctggggca gtggctccgc ggccccgccc cgcagacgtc
     6301 gcccggcccg ctcacccgcc tgcgccgggc cctgcggccc gcccgccggc accactgacc
     6361 cgatctccgc ttcattcacc acccggggga atccgcccat gtggcacaac acgtacttcc
     6421 tcgtaccagc cgtcctgctg gtcgccgccg cgctctacgc accgcagacc aagcaggcgg
     6481 cccagagggt cccgcaggcc aagggcctgc ccgggcgcgg gctgccgctg ctcgacaccg
     6541 ccttcctctc cggcgggccc ggccgggtct tcgacaccgc gttcgtccgg atgcaccagt
     6601 cgggccggct cgtcgtcaac cgggacggcc tggtcacgct gaccggcggc cggccgtacg
     6661 actccgtcga ggaggcgatc atcgaggccc tcggcccggc cgggcagcgc gagctggccc
     6721 cgctgcggcg cgcggtgatg cgctcggccg ccgtccagaa gctgggcgac gggctcgccc
     6781 accgcgggct gctgcgcaac cccgtcctgc tgcgccgggc caaggtggcg cgccgaccgc
     6841 tctggctggc cctgctggtc gtggtgctga gcaccgtcct cgcctacgtc ctggacaacg
     6901 gcgaagcgga ccgcccggcg ctctggatcc cggcggtcct gctgatcgtc ggactggcgt
     6961 cagtgatcgt ctcctggccg ctcaagggcc ggatcacgcc ggccggacgc cgccagctcg
     7021 ccctgatgga caccggcaag ccgtggacgc cggccgacgc cgtggcgccc ctggcgaccg
     7081 gcgccgcgct gctcggcgcg atcgcgctcg gcgggctggc cgcggccggg ctggaggacg
     7141 cggagctcca ggaggcgatg ctcgccgccg cggcggccga ggaggccgcc gccagggccg
     7201 ccgccggcgt gtcctcctcc tcggcgtcct cctccggttc ctcctgctcc agcccggcca
     7261 cctggtgcgg tgcttcgacc gactccggct cctccggttc gggctgcggc tcctccagcg
     7321 gttgcggttc ctcctccagc agctgcggct cgtcctcctc gtcaagttgc ggctccagct
     7381 ccagcagctg cggctcctcg tcctcctcca gttgcggttc cagctgcggc ggcagctcct
     7441 gcggctcgta gggtccgtcc tcgaactccc accggcctcc cgagtcacag ttcggtatcg
     7501 cggtccggga atccgttccg cccggagcga aggcgggtct acaacaagcc catgtggttg
     7561 ctcttcctga tcccggcctg cgccgccgcg gtgctctcct gcatccggct ggtccggatg
     7621 gccgccaccg ccgacgcgct cgccgaccag gagggccccc ggccgcccga ggcggtcggg
     7681 atcggcctgt acgagacggc ctacctggcg ggcggaccgg accgggtggt cgaactggca
     7741 ctcgtcctga tggcctcccg gggccggctg cacctcgccc acaccggctg gaccaccgtg
     7801 gtcgatccga agggccgcag ccggctggag cgggccgtca tcgccaccgt cggcccggac
     7861 gggcagtgcc gcaccgacga cctgcgccgg gcgatggccg cccacccgac cgtgctggag
     7921 atcgggggcc ggctcgccct ggccggcctg gccaccccgg caccggtcca gcagagcacc
     7981 gtcctggtga tccggcaggt ccggcaggcg ctgctgctct cgctggtcct gatggccggc
     8041 gccgtggccg tcggcggcct ctcggacggt gacgcgctgg cgccgctcgc ctggttctcg
     8101 ctgccgctgg tcctcaccgc gggcaccctg ctgatggccc gggtcgacgt ctacccgtac
     8161 acccgctggg cctcccccat cggccaggag gtcctgcgcg aggtccgccc gccccgccag
     8221 cacggcctcg gcgacgacga cgagcgcgaa ctgctcaccg ccgtcgccat gatcggcccc
     8281 gccgccgtcc cggacccgcg cctgcgcgcg gccctgcgcc accggcactg accggaaacc
     8341 cggtgcgccg cccggcctcc cccggcagac tcgcggcatg aggcgaaccc cgggccccgc
     8401 ctcgcccgtc cccgccctcg acctggccgt acgggacggc gggctggtcc ggctgcaccg
     8461 ctcgctgccg ggcgccgacc gcctggaggg cttcgtcgtc ggcaccgccg ggggtcaccg
     8521 gacacgccga gggccggctc ccccttcccg ggggaaccgg ccctcgttcg gccgggctca
     8581 gaccaggccg gccagcagct cgctcaccgg cttgcggcgg ccggtgaaga acggcacctc
     8641 ctcgcggacg tgcagccggg cgcgggacgg gcgcaggtcg cgcatcaggt cgacgatccg
     8701 gtgcagctcg tccgcctcga aggcgagcag ccactcgtag tcgccgagcg cgaacgaggc
     8761 caccgtgttg gcccgcacgt cggggtagcc gcgcgccatc tggccgtgct cggcgagcat
     8821 cgcgcgccgc tcggcgtccg gcagcaggta ccactcgtag gagcggacga acgggtagac
     8881 gcagacgtag tcgcgcgggc gctcgtcggc gaggaacgcc gggatgtgcg acttgttgaa
     8941 ctcggccggg cggtgcagcg ccatgttcga ccagaccggc tccagctcac ggcccaggcc
     9001 ggtgcggcgg aagcggttgt acgcctcctg gaggtcgtcc gagtccgccg cgtgccacca
     9061 gaccaggatg tcggcgtccg cccgcagacc ggacacgtcg taggtgccgc gcaccgtgac
     9121 gtccttcgcg gcgagctggt cgaacagctc gtccacctcg gcggccagcg cggtgcggtc
     9181 ctcgggcagc gcgcccttga gcttgaacac cgaccacatg gtgtagcgga tgacctggtt
     9241 caggtcgcgc gccttcttct tctcgggctg ctgctccggc tgctgctcaa cggtctcagt
     9301 catgcgccca ttgtcctctc cgtgacgttc tccccggtca gcggggtcaa cactttgtcg
     9361 agcgcgtcga cggccgtccg cgcgctcgcg atgcacgccg ggatgcccac cccgtcgtac
     9421 gcggccccgc agagcgccag cccgcccacc cggccggccg ccgcccggat ccgcgccacc
     9481 cggtcgaggt ggccgaccgg gtactgcggc aggccggccc gccagcgggt gacggcggtg
     9541 tcgtacggcc gggcggtcag gccgatcgcc tcgcccaggt cggccagcga ctgcgccacc
     9601 agctcctcgt ccgacaggtc cagcgcctgc tcctcgtgca gccggcccag cgaggtccgc
     9661 agcaggaagg tgtccggggc cgaacgctcc agccagcccc acttgttgga ggagaaggtg
     9721 gacgccttga tccggcggcc gtccaccggc ggcaccagga acccgctgcc ggccggcggc
     9781 tccggcaggt cggcccgccg gaacgccagg gtgaccagcg ccatccccgc gtactcgacg
     9841 ccgtccagct cggcggccgc ccccggcacg tcggtgcgca gcagccgggc ggcggcgggc
     9901 gcgggcacgg ccagcaccac ggcgtccgcg tgcagcgtcg cgccgtcgcc ggtcaccacc
     9961 cgccagccgt ccgcggtgcg ccgcagctcc tccacggcgg tccgggtgcg cagctcgacg
    10021 ccggcgtcgg cgcaggccgc ggcaaccgcc ccgggcaggg tgccgatacc gccgcgcagg
    10081 ccctggaaga cgggggtcga gacggtctgc ggccgggtgg ccagctcgtg cacgccctcg
    10141 accagcgagc cgccgccacg ggcgatcgcc agcagctgcg gcacggcggc gcgcagcgag
    10201 atctcgtccg cgtgcccggc gtagacgccg cccagcagcg gctcgaccag ccggtcgacc
    10261 acctcgcggc ccagccggtc ggcgacgtag cgcccgatcg cgacgtcgtc cgcgacgccc
    10321 tcgccggagc cctcgtgccg ggcccgttcc agcccctcgg cggtcagcac cccggacttc
    10381 gcgagcgcct ccaggtcacc cgggacgccc atcacctgcc cgccgggcag cggccgcaac
    10441 gcgccgcgcg tccagatcgc ggccttggcg gtggtcggcg gctccagctc cgcgcccagc
    10501 ccgaccgccc gcgccagctc gaccgcctcc ggtcgccggg ccagcatcga ctcggccccg
    10561 agatccaccc ggaccccgcc gacctcgccg cccgacagct tcccgccgag ccgctccgac
    10621 gcctccaaca gcgtcacccg ggcccgcgcg ggcccgcctg ccgccccact gagaaacgcg
    10681 gccgcggcca gccctgcgat accgccaccg atcaccacga cctgtgcttg cgccatgtcc
    10741 gcagtctcgc aggtccttac atcctcgcct cagtccgccc gctccgggcg cttcgccgcc
    10801 ggcacgagcg cgtcgaggtc gatgtccacg gggaacggca cgggccgctc cagccgcccc
    10861 cggaagatcc cggcgggggc gtaggaggag gtggcggtgt cgagctcgta ggcgtgcacg
    10921 accggccgtc cgtcctcgtc ctcgatcacc cagtagtgcg ggatgccggc ctcggcgtac
    10981 ttgcgcacct tgacggtgcg gtcgcggtgc gcggactccg gggagaccac ctcgacgacg
    11041 agcgccacgt ccccggccgc gtaccaggtg cggtcggggt cgtagtcggc ggtcgagacc
    11101 agaacgtcgg gctccggccg gttccgccgg tccagcttga tcgtcatctc gcgccccgcg
    11161 cgactgcccg gcgggacctg ggcccgcagc gcgacgacca ggtcggtgac gagctcaccg
    11221 tgccaccaac gctgcgggga cagcctgaag accagtgctc cgtcgatcag ttcggtgtgc
    11281 cgcggcgcct gcggcaggtg gtcgaggtcc tcggcgatcc acccctcgtc ccggggcggg
    11341 tacatccagt ccggcagc
//
