LOCUS       NZ_CP012673            10882 bp    DNA     circular CON 30-MAR-2024
DEFINITION  Sorangium cellulosum strain So ce26 chromosome, complete genome.
ACCESSION   NZ_CP012673
VERSION     NZ_CP012673.1
KEYWORDS    RefSeq.
SOURCE      Sorangium cellulosum
  ORGANISM  Sorangium cellulosum
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae;
            Sorangium.
REFERENCE   1  (bases 1 to 14557589)
  AUTHORS   Zaburannyi,N., Bunk,B., Overmann,J. and Mueller,R.
  TITLE     Sorangium comparison
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14557589)
  AUTHORS   Zaburannyi,N., Bunk,B., Overmann,J. and Mueller,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-SEP-2015) Department of Microbial Natural Products
            (MINS), Helmholtz Institute for Pharmaceutical Research Saarland
            (HIPS), Campus E 8.1, Saarbruecken, Saarland 66123, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT portal v. 2.2.0, HGAP v. 3.0
            Genome Coverage                   :: 134x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002950945.1-RS_2024_03_29
            Annotation Date                   :: 03/29/2024 11:58:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10,531
            CDSs (total)                      :: 10,457
            Genes (coding)                    :: 10,397
            CDSs (with protein)               :: 10,397
            Genes (RNA)                       :: 74
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 6 of 60
            Pseudo Genes (incomplete)         :: 58 of 60
            Pseudo Genes (internal stop)      :: 2 of 60
            Pseudo Genes (multiple problems)  :: 6 of 60
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 02:39:47
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5692366
            Orig. end                         :: 5703248
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP012673.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria available from German Collection of Microorganisms and
            Cell Cultures GmbH, Inhoffenstrasse 7B, 38124 Braunschweig,
            Germany.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10882
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="17"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10882
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10882
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5882](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5882)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(195..1580)
                     /locus_tag="SOCE26_RS20770"
                     /old_locus_tag="SOCE26_041710"
     CDS             complement(195..1580)
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_M50"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236060.1"
                     /locus_tag="SOCE26_RS20770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SOCE26_041710"
                     /product="site-2 protease family protein"
                     /protein_id="WP_104981505.1"
                     /sec_met_domain="Peptidase_M50 (E-value: 9.8e-07, bitscore:
                     27.5, seeds: 56, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRMSFRLFGVPVDVQLGFWLSAALLGFGRLVAEQYQRFLVWIIVV
                     FLSVLMHEFGHALAIKRHRIEPEITLHFMGGTTTWRSLMPLGRLQHVIISLAGPFAGFF
                     VAGILYLLLSYVPALHALPPMLIYGMDTLIWVNVVWGVLNLIPVLPFDGGHVLEHALGP
                     KRGRLTAAISGVAAVLLAIVFLQMGFIFFTFILVMSAFQSLQRFRSEPAASSPVVNRRR
                     AALQDEPLPPETEQLLQRARRAVEDERTDEARALAGEVLAQSPAPPKRALREAHELIGW
                     AHLLLGDTKAAVDAVAQARKHGEPDPALVGAVHLAQGELKQARKVLEAAREAGDDRKEI
                     AGPLIRVLVEQGEVARAAAVALDIVEQLSEDDARRMAEIAFERGAFDWSARLYEAIFRR
                     IGQSEDAYDAARASAKDGNLDRALELLRDAVEAGFSDRARAWSDSAFEALRGGPLETVL
                     PRP"
     gene            1744..2601
                     /locus_tag="SOCE26_RS20775"
                     /old_locus_tag="SOCE26_041720"
     CDS             1744..2601
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236061.1"
                     /locus_tag="SOCE26_RS20775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SOCE26_041720"
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="WP_104981506.1"
                     /transl_table=11
                     /translation="MGRDIRGTRWALAGISAAGIACGAAPPPEPDAAGPPAGEPPPAAT
                     RPLEAAGPVAPPPAAPAAGEPQPAAEPPPFSVPPNTAVLHVGDSFALAGFAQALRPRLE
                     ALRVRYAVKAETSSYTVTWASRMELLVANYQPDLVIISLGANEVENVNPPAHAGAVRRI
                     VKAIGGRPCVWVSPPLWRKDTGIIDVIRTNSAPCRFFDSDALVPGPLPRKKDKIHPNEV
                     GGARWADAFWTWLTAEHLPPGAGEGADTSAPPRSPWALRPAPPEEHRSRAPAPPPENAL
                     QGAL"
     gene            complement(2745..3500)
                     /locus_tag="SOCE26_RS20780"
                     /old_locus_tag="SOCE26_041730"
     CDS             complement(2745..3500)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1141: acyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236062.1"
                     /locus_tag="SOCE26_RS20780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SOCE26_041730"
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="WP_104987335.1"
                     /transl_table=11
                     /translation="MSLHTTCMGIFDTLRICFPTVVDAVRGRVTLERCDERLDWWARKV
                     LEDADVDLVVRGREHAAGTRPFVVMSNHQSLFDIPVVFRAIPGRLRMVAKTELFKLPVF
                     GQAMKAAGFIRIDRSDRVQATASLQVGAAMLQNGTRVWIAPEGTRSKTGALLPFKSGGF
                     RMAIETNTPILPVAIDGTRHVLRARDVVVQQHHRVVVTIMPPIDPAPYGQGGRNRLMRD
                     VRAAIAGALGHDPSLVHRSAATTVAAPPA"
     gene            complement(3636..4067)
                     /locus_tag="SOCE26_RS20785"
                     /old_locus_tag="SOCE26_041740"
     CDS             complement(3636..4067)
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_process="GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236063.1"
                     /locus_tag="SOCE26_RS20785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SOCE26_041740"
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="WP_104981507.1"
                     /transl_table=11
                     /translation="MITIVQRTLEVRTSGRGFFDITREVARVVAGAGVDVGTCALFLQH
                     TSASLVIQENADPAVLRDLERWMSRLAPEGRDYEHDDEGPDDMPGHLRSAVTRSSEVIP
                     VSGGRLALGTWQAVYVWEHRSSPHMRRVVVTVTGTTAGG"
     gene            complement(4064..5017)
                     /locus_tag="SOCE26_RS20790"
                     /old_locus_tag="SOCE26_041750"
     CDS             complement(4064..5017)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736097.1"
                     /locus_tag="SOCE26_RS20790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SOCE26_041750"
                     /product="DNA-binding domain-containing protein"
                     /protein_id="WP_234023844.1"
                     /transl_table=11
                     /translation="MRAPHDDGRPAAGRTAPAGRDAPDDAPPPAGLVAMMDELTRLITR
                     GAPLPDDAELAARCGALVAGSARLTPAEQADIYRRQFWARHTDALLEDHPGLAHVLGED
                     GFDAFARAYLAAHPPARFSLRDLGEHLADFIKGYDGFPPALRDLARDMARYELALVDVF
                     DGAAPPPLDPAKVTGMPDEGWTTARLVLHPLLVLMDLRYPVHLLRSELRQGKKPALPGP
                     RPVRLAVFRSADLTIRYEELEPPAFALLHALGRGAPLVAACEEVAAGVSDAAGADEAMR
                     TALAARLGTWFQQWTSWGLIVDIAPGSRAGDAKMAR"
     gene            complement(5001..5882)
                     /locus_tag="SOCE26_RS20795"
                     /old_locus_tag="SOCE26_041760"
     CDS             complement(5001..5882)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236065.1"
                     /locus_tag="SOCE26_RS20795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SOCE26_041760"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_234023845.1"
                     /sec_met_domain="DUF692 (E-value: 9.7e-98, bitscore: 325.9,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MANLDALDSTRLGSMRLGLPDLGVGVGLRIPHYRHVFEHRPRVDW
                     FEIISENFMVDGGMPIANLERALQSYRLVQHGVSLSIGSTDPLDVDYLRRLKALLTRVG
                     SPWVSDHLCWTGAHGVNLHDLLPLPYTEEAVRHVAARARAVQDFLGVRLALENVSSYLT
                     FTASRMTEWEFLSAVAEEADCGILLDVNNVYVSSYNHGFDPNAYIDGVPHRRIVQFHLA
                     GHTHHGKYILDTHSDRVDPAVWALYRRALGHTGDVSTLIEWDDDLPPFEDLAAEVEKAR
                     AIREEVARARAA"
     gene            complement(5959..6279)
                     /locus_tag="SOCE26_RS20800"
                     /old_locus_tag="SOCE26_041770"
     CDS             complement(5959..6279)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736099.1"
                     /locus_tag="SOCE26_RS20800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SOCE26_041770"
                     /product="hypothetical protein"
                     /protein_id="WP_104987338.1"
                     /transl_table=11
                     /translation="MATNKVEVYMAMDMGKTLAFTAVSGMVAGLAGCASSPPPEPETGA
                     PPAEPAAEAPTAGAKACCAGKNECKGKGGCKSDTHSCAGQNECKGKGGCKPADCGGGHD
                     HH"
     gene            6653..7648
                     /locus_tag="SOCE26_RS20805"
                     /old_locus_tag="SOCE26_041780"
     CDS             6653..7648
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736100.1"
                     /locus_tag="SOCE26_RS20805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SOCE26_041780"
                     /product="hypothetical protein"
                     /protein_id="WP_104981508.1"
                     /transl_table=11
                     /translation="MAALPLAAALSLGAAGCGGAPRAAAGAEGAGVPVGELPIEAVAAE
                     LEAMSGAVLEGREATFRDILADTVAKDMYLCVPSARQVFHGDVPEGERRIAGAMPHYGL
                     FFGPMRYLVRRRGGAWEVEVRVAVEPPAGAGRLELPDCGLAAQLGEALTCRGTPYARSG
                     STDACPGSGEFSAAATPAAIRALLARWSAEVEGYWNRDARAFALPVRYDFDFVLESEAR
                     ARALRVDFAVPLSPTCGRTPYFYAIRAGWSLPVLAHEVGHVLGLLDEYEALSGIVRCYP
                     KTPFPGAEISRMGLSMREGTRLLPVHHYLVLRRFFCREPSSTDPYAGAIR"
     gene            7725..8711
                     /locus_tag="SOCE26_RS20810"
                     /old_locus_tag="SOCE26_041790"
     CDS             7725..8711
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="SOCE26_RS20810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SOCE26_041790"
                     /product="hypothetical protein"
                     /protein_id="WP_159397131.1"
                     /transl_table=11
                     /translation="MGRWLAGAALAGMALAGCRSERDGGAADAGASAADAGAGASDAGA
                     VEAAPPMIGAPRAPRAPWPPAEATGPAPPAEPRCPPEMVRVARRFCVDRYEAVLLDKET
                     GLEISAFYPPSRREAASVERAWSRLRFQMGDAEARRMELPLLPGWQKERDFVPRAVPRR
                     GVLPQGYTSGAQAELACRNAGKRLCTLEEWRTACRGERDEPFPYGPTYEDGRCNVHREA
                     HPAGVLHRDVSLGHSDPRLNLVRVAGAPLLRRTGETATCASVWEDDAVSDMVGNLDEWV
                     DDPEGTFAGGFYARATKQGCMATIASHDFTYFDYSTGVRCCADLRGP"
     gene            8806..9282
                     /locus_tag="SOCE26_RS20815"
                     /old_locus_tag="SOCE26_041800"
     CDS             8806..9282
                     /NRPS_PKS="Domain: Polyketide_cyc2 (6-151). E-value:
                     1.2e-12. Score: 40.3. Matches aSDomain:
                     nrpspksdomains_SOCE26_RS20815_Polyketide_cyc2.1"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /locus_tag="SOCE26_RS20815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SOCE26_041800"
                     /product="SRPBCC family protein"
                     /protein_id="WP_159397132.1"
                     /transl_table=11
                     /translation="MIQARYSVYDSAVLDAPIEEAWRELRDIVKLLPLVFGDSVKDYRY
                     VDGGSAEKIPSRFEFTLLPSGETGLEEVVARSETEHSLTYRMLGQLIGVEGYIATYRLR
                     RITTEPGKTFLEWPREFGVAAGADPAEVVPAIEALTAREVATIKEHFEKRRRSL"
     aSDomain        8824..9258
                     /aSDomain="Polyketide_cyc"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SOCE26_RS20815_Polyketide_cyc2.1
                     "
                     /domain_subtypes="Polyketide_cyc2"
                     /evalue="1.20E-12"
                     /label="SOCE26_RS20815_Polyketide_cyc2.1"
                     /locus_tag="SOCE26_RS20815"
                     /protein_end="151"
                     /protein_start="6"
                     /score="40.3"
                     /tool="antismash"
                     /translation="SVYDSAVLDAPIEEAWRELRDIVKLLPLVFGDSVKDYRYVDGGSA
                     EKIPSRFEFTLLPSGETGLEEVVARSETEHSLTYRMLGQLIGVEGYIATYRLRRITTEP
                     GKTFLEWPREFGVAAGADPAEVVPAIEALTAREVATIKEHF"
     misc_feature    9371..9384
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 17.82"
                     /tool="antismash"
     misc_feature    9614..9629
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.74"
                     /tool="antismash"
     misc_feature    10136..10145
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.0"
                     /tool="antismash"
     misc_feature    complement(10142..10155)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.11"
                     /tool="antismash"
     misc_feature    10335..10349
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.19"
                     /tool="antismash"
ORIGIN
        1 ccgtcgggca aggcgaccgc gtaccccgtg ggcacgagct tccgctcccc cggcccgatc
       61 gtcacctccg cgtcgatcgc cgcgcggagg tcgagcccca cggccccctc ggactggtaa
      121 gcgggcggcg ggacctcgat aggacctaca cgaaggaacg agacctggat cggcacgcgg
      181 ccgagactac cccgtcacgg ccgcggcagc accgtctcga gcggcccgcc ccggagcgcc
      241 tcgaacgccg agtccgacca cgcccgcgcc cgatccgaga agccggcctc caccgcgtcc
      301 cggagcagct ccagcgcccg gtccaggttc ccgtccttgg cgctcgcgcg cgcggcgtcg
      361 taggcgtcct cgctctggcc gatgcggcgg aagatcgcct cgtagagccg cgccgaccag
      421 tcgaacgcgc cgcgctcgaa cgcgatctcc gccatcctgc gcgcgtcgtc ctccgagagc
      481 tgctccacga tgtcgagcgc gaccgccgcc gcgcgcgcca cctcgccctg ctcgacgagc
      541 acccggatca gcgggccggc gatctccttc cggtcgtcgc cggcctcccg cgccgcctcg
      601 agcaccttgc gcgcctgctt gagctcgccc tgcgcgaggt gcaccgcgcc gaccagcgcc
      661 gggtccggct cgccgtgctt gcgcgcctgc gcgaccgcgt ccaccgccgc cttcgtgtcg
      721 ccgaggagca ggtgcgccca cccgatgagc tcgtgcgcct cccgcagcgc gcgcttcggc
      781 ggcgccggcg actgcgcgag cacctcgccg gccagggccc gcgcctcgtc cgtccgctcg
      841 tcctccaccg cccgccgcgc ccgctggagc agctgctccg tctctggcgg caggggctcg
      901 tcctggagcg ccgcgcgccg gcggttcacg accggcgacg acgccgccgg ctcgctgcgg
      961 aaccgctgca ggctctggaa cgccgacatg acgaggatga acgtgaagaa gatgaacccc
     1021 atctggagga agacgatcgc gagcagcacg gccgccacgc ccgagatcgc cgccgtgagc
     1081 cgcccccgct tcgggccgag cgcgtgctcc aggacgtgcc cgccgtcgaa cggcagcacg
     1141 gggatcaggt tgaggacgcc ccacaccacg ttgacccaga tgagcgtgtc catcccgtag
     1201 atcagcatcg gcgggagcgc gtggagcgcc gggacgtagg agagcaggag gtagaggatg
     1261 ccggcgacga agaagccggc gaacgggccg gccaggctga tgatcacgtg ctggagccgc
     1321 ccgaggggca tgagcgaccg ccaggtcgtc gtgccgccca tgaagtgcag cgtgatctcc
     1381 ggctcgatcc ggtgccgctt gatggccagg gcgtgaccga actcgtgcat cagcaccgac
     1441 aggaagacca cgatgatcca cacgaggaac cgctggtact gctccgcgac cagcctcccg
     1501 aacccgagca gggcagcgga gagccagaac ccgagctgga cgtcgaccgg aaccccgaac
     1561 aggcggaagc tcatgcgcat gtaataagcc ctggaggccc tctgctcaat gattgccgta
     1621 cggactcggc ctgccctaga gtgcgccgcc gcgccgcgcg ctgcgtggac gacggcgctc
     1681 gcggcgtgcg tggacgaaga cgctcgcggc gcacgcgccg ggcgcggtct tctggagcgg
     1741 accatgggca gggacattcg gggtacgagg tgggcgctcg ccgggatctc cgcggcgggc
     1801 atcgcgtgcg gcgccgcgcc gccgcccgag ccggacgcag cgggtccgcc cgccggcgag
     1861 ccgccgcccg cggccacccg cccgctcgag gcggccgggc ccgtcgcgcc gccgccggcc
     1921 gccccggcgg ccggcgagcc gcagcccgcc gcggagccgc cgccgttcag cgtgccgccc
     1981 aacacggcgg tcctccacgt cggcgactcg ttcgcgctcg cgggcttcgc gcaggcgctc
     2041 cgcccgcggc tggaggcgct gcgcgtgcgc tacgcggtca aggcggagac cagctcgtac
     2101 accgtgacgt gggcctcccg gatggagctc ctggtcgcga actaccagcc ggacctcgtg
     2161 atcatcagcc tcggcgcgaa cgaggtcgag aacgtgaacc cgccggccca cgccggcgcg
     2221 gtccggcgca tcgtcaaggc gatcggcggg cgcccgtgcg tgtgggtctc cccaccgctc
     2281 tggcggaagg acaccggcat catcgacgtg atccggacga actcggcccc gtgccggttc
     2341 ttcgactccg acgcgctggt gcccggcccg ctcccccgca agaaggacaa gatccacccg
     2401 aacgaggtgg gcggcgcgcg ctgggccgac gccttctgga cctggctcac ggcggagcac
     2461 ctgccgccgg gagccggtga gggcgctgac acgtccgcgc cgccgcgcag cccctgggcg
     2521 ctccgccccg cgccgcccga ggagcaccgc tcccgggcgc cagcgccgcc gccggaaaac
     2581 gcgctccagg gcgcgctcta gagcgacaaa cgggttgaac ccgtctgaaa cgagcagatt
     2641 ttcgaaccgc caaggcgcca aggacgccaa gaaggagaag aaaatatctt ggcgcccttg
     2701 gcgccttggc ggttcatctc tcgttcctca acgtgatcgg tgttctaggc gggcggggcg
     2761 gcgacggtgg tggcggccga tcggtggacg agggacgggt cgtggccgag cgcccccgcg
     2821 atcgccgcgc ggacgtccct catgaggcgg ttgcggccac cctggccgta gggcgccggg
     2881 tcgatcggcg gcatgatcgt caccaccacg cggtggtgct gctgcaccac gacgtcgcgg
     2941 gcccgcagca cgtggcgcgt gccgtcgatc gcgaccggga ggatcggcgt gttggtctcg
     3001 atcgccatcc ggaagccgcc gctcttgaag gggaggagcg cgcccgtctt gctccgggtg
     3061 ccctccgggg cgatccagac ccgcgtgccg ttctgcagca tggcggcgcc gacctggagg
     3121 ctcgccgtgg cctggacacg atcgctccgg tcgatgcgga tgaacccggc cgccttcatc
     3181 gcctggccga agacgggcag cttgaacagc tcggtcttcg ccaccatccg gagccgcccg
     3241 gggatggcgc ggaacaccac cgggatgtcg aagaggctct ggtggttgga catcaccacg
     3301 aacggccgcg tgcccgcggc gtgctcgcgc ccgcgcacca ccagatcgac gtcggcgtcc
     3361 tccaggacct tgcgcgccca ccagtcgagc cgctcgtcgc agcgctccag ggtcacgcgc
     3421 ccccggaccg cgtccacgac ggtggggaag cagatccgga gggtgtcgaa gattcccatg
     3481 cacgtcgtgt gaaggctcat cgctgtgatt ccgctgaagg tgaagggggt cgccaggtcc
     3541 acccacgccg cgcgcaggtg ctgccgggcg cggccgctga gcagcccccg agggggtcga
     3601 gctcatccag gacatggacg cggcaagtat accacttacc cccctgctgt ggtgccggtt
     3661 acggtaacaa ccacgcgacg catgtggggt gagctacgat gttcccagac atacacggct
     3721 tgccatgttc cgagcgcgag gcgaccgccc gagacgggga tgacctcgct cgaccgggtg
     3781 acggcgctcc ggaggtggcc cggcatgtcg tcggggccct cgtcgtcgtg ctcgtagtcg
     3841 cggccttccg gggcgaggcg cgacatccag cgctcgaggt cgcggaggac cgccgggtcg
     3901 gcgttctcct ggatgaccag gctcgccgag gtgtgctgga ggaagagggc gcaggtcccg
     3961 acgtcgacgc cggcgccggc gacgacgcgc gcgacctcgc gcgtgatgtc gaagaacccg
     4021 cggccgctcg tgcggacctc gagggtgcgc tggacgatgg tgatcaccgc gccatcttag
     4081 cgtccccggc gcgggatccc ggcgcgatgt cgacgatcag gccccagctc gtccactgct
     4141 ggaaccaggt cccgaggcgg gccgccagcg ccgtgcgcat ggcctcgtcc gcgcccgcag
     4201 cgtcgctcac gccggccgcc acctcctcgc acgcggcgac gagcggcgca ccgcgaccga
     4261 gcgcatggag cagcgcaaac gccggcggct cgagctcctc gtagcggatc gtgagatcgg
     4321 cggagcggaa gacggcgagg cggacggggc ggggcccggg cagcgcgggc ttcttcccct
     4381 gccggagctc gctgcgcagg aggtgcaccg ggtaacggag atccatcagc acgagcagcg
     4441 gatggaggac gaggcgggcg gtggtccagc cctcgtccgg catgcccgtc accttcgcgg
     4501 gatcgagcgg tgggggcgcg gccccgtcga agacgtcgac gagggcgagc tcgtagcgcg
     4561 ccatgtcgcg ggcgaggtcg cggagggcag gggggaagcc atcgtaaccc ttgatgaaat
     4621 cggcgaggtg ctccccgagg tcgcgcagcg agaagcgcgc cggcgggtgc gcggcgaggt
     4681 aggcccgggc gaaggcgtcg aagccgtcct cgccgaggac gtgcgcgagc ccggggtggt
     4741 cctcgagcag ggcgtcggtg tgacgcgccc agaactgccg ccggtagatg tcggcctgct
     4801 cggcgggcgt gagccgcgcg ctgccggcca cgagcgcgcc gcaacgcgcg gcgagctcgg
     4861 cgtcgtccgg gaggggcgcg ccgcgcgtga tcaggcgggt gagctcgtcc atcatggcga
     4921 cgagcccggc cgggggcggg gcgtcgtccg gggcgtcgcg cccggccggg gcggtgcgcc
     4981 cggccgcggg tcggccgtcg tcatgcggcg cgcgcacgcg ccacctcttc ccggatcgcg
     5041 cgcgccttct ccacctcggc ggcgaggtcc tcgaacgggg gcaggtcgtc gtcccactcg
     5101 atcagcgtgg agacgtcgcc ggtgtggccc agcgcgcgcc ggtacagggc ccagacggcg
     5161 gggtccacgc gatcgctgtg ggtgtcgagg atgtacttgc cgtggtgcgt gtggccggcg
     5221 aggtgaaact gcacgatccg gcggtggggc acgccgtcga tgtaggcgtt cggatcgaag
     5281 ccatggttgt acgaggagac gtagacgttg ttgacgtcga gcaggatgcc gcagtcggcc
     5341 tcctcggcga ccgccgagag gaactcccac tcggtcatcc ggctcgcggt gaaggtcagg
     5401 taactcgaca cgttctccag ggcgagccgg acgccgagga agtcctgcac cgcgcgggcg
     5461 cgcgccgcga cgtggcgcac cgcctcttcc gtgtagggca gggggagcag gtcgtggagg
     5521 ttgaccccgt gggcgccggt ccagcacagg tgatcgctca cccacgggga gcccacgcgg
     5581 gtaagcagcg ccttcagcct gcggaggtag tcgacgtcga gcggatccgt gctgccgatc
     5641 gagagggaga cgccgtgctg gacgagccgg tagctctgga gggcgcgctc caggttcgcg
     5701 atcggcatgc cgccgtcgac catgaagttc tcgctgatga tctcgaacca gtcgacgcgg
     5761 ggccggtgct cgaagacgtg gcggtaatgc gggatgcgca gcccgacgcc gacgccgagg
     5821 tcgggcagcc cgagccgcat ggacccgagc cgcgtggagt cgagggcgtc gaggttcgcc
     5881 atgggtcacc gccgctcccg cggctgcccg gggcccggcg ccggcgcgca ccgctcgcgc
     5941 gccgtgcgcc gccgcggatc agtggtggtc gtgtcccccg ccgcagtccg ccggcttgca
     6001 gccgcccttg cccttgcact cgttctggcc ggcgcacgag tgggtgtccg acttgcagcc
     6061 gcccttgccc ttgcactcgt tcttgccagc acagcacgcc ttcgcgccgg ccgtgggcgc
     6121 ctccgccgcc ggctcggccg ggggagcccc cgtctcgggc tcgggcggcg gcgacgaggc
     6181 acagcccgcg aggcccgcca ccatgcccga aaccgccgtg aacgccaacg tcttgcccat
     6241 gtccatcgcc atgtaaacct ccaccttgtt cgttgccatc gagctcgcgc gcgcttgctg
     6301 cctcggcgcg ccgtcgatcc gctgcgctgc tcggggctcg tcggtctggc cggtcgcggt
     6361 gcacccgcgc tccacccgca ctctacgccc gcgcggccgc tcggtttcac cggtccgcgc
     6421 gggtgcgttg attcctggtg cgactccccg taagtgagcc ccgttgacca ccacgcctac
     6481 gcgaagctcc ctcgcgcgcg cggcgcccgg cgacgtgtct cccggggcct ggacgcccgt
     6541 ccgccccgca gcattccacg tacgacgtag ctcgtttcgc gcgacggggg caagacaaag
     6601 cggccgctgc cgcgcgccgt ggccgcctcc gcgcgggctc gtttaccggc gcgtggccgc
     6661 gcttccgctc gcggccgcgc tgtcgctggg ggcagcgggc tgcggagggg ctccacgcgc
     6721 ggcggccggc gccgagggcg ccggggtccc ggtgggcgag cttccgatcg aggcggtcgc
     6781 cgccgagctc gaggcgatga gcggcgcggt cctcgaaggg cgcgaggcga cgttccggga
     6841 catcctggcc gacacggtgg cgaaggacat gtacctgtgc gtgccctcgg cgcggcaggt
     6901 gttccacggc gacgtgccgg agggggagcg gcgcatcgcc ggggcgatgc cgcactacgg
     6961 gctgttcttc ggcccgatgc gctacctcgt gcggcggcgc gggggcgcct gggaggtcga
     7021 ggtgcgcgtc gccgtggagc cgccggccgg cgcgggccgc ctcgagctgc cggactgcgg
     7081 gctggccgcg cagctcgggg aggcgctcac gtgccgcggg acgccgtacg cgcgctccgg
     7141 atcgacggac gcctgcccgg gctccgggga gttctcggcg gcggcgacgc cggcggcgat
     7201 ccgggcgctg ctcgcccgct ggtcggccga ggtcgagggg tactggaacc gcgacgcgcg
     7261 cgcgttcgcg ctgccggtcc ggtacgactt cgacttcgtg ctcgagagcg aggcgcgcgc
     7321 gcgggcgctg cgggtcgact tcgcggtccc gctctcgccg acctgcggga ggacgccgta
     7381 tttctacgcg atccgggccg gctggtcgct gccggtgctc gcccacgagg tggggcacgt
     7441 gctcgggcta ctcgacgagt acgaggcgct ctccggcatc gtccgctgct acccgaagac
     7501 gccgttcccg ggcgcggaga tcagccggat ggggctgtcg atgcgggagg ggacgcggct
     7561 cttgccggtc caccactacc tcgtgctccg ccgcttcttc tgccgcgagc cgtcgtccac
     7621 ggatccctat gccggcgcga tccgctgacc ggcacgtccg gcctcgggac ggccgccggg
     7681 gtgcggcgac cgcggcgggc ggggcgctcc caggcggggc gctggtggga aggtggctgg
     7741 cgggcgcggc gctcgcgggc atggcgctcg cgggctgtcg ctcggagcgg gacggcggcg
     7801 cggccgacgc gggcgccagc gcggccgacg ccggcgcggg ggcgagcgac gccggcgcgg
     7861 tggaggcggc gccgccgatg atcggggcgc cgcgcgcgcc gcgggcgccg tggccgccgg
     7921 ccgaggcgac cgggccggcg ccgcccgcgg agccgcgttg cccgccggag atggtgcggg
     7981 tcgcccgccg cttctgcgtg gatcgctacg aggcggtgct gctcgacaag gagaccgggc
     8041 tcgagatctc ggccttctat ccgccgtcgc ggcgggaggc cgcgtcggtc gagcgcgcct
     8101 ggtcgaggct gcggttccag atgggcgacg ccgaggcgcg gcgcatggag ctgccgctcc
     8161 tgcccggctg gcagaaggag cgcgacttcg tgcctcgcgc cgtgccgcgg aggggcgtgc
     8221 tcccgcaggg ctacacgagc ggcgcgcagg cggagctcgc gtgcaggaac gccggcaagc
     8281 ggctctgcac gctcgaggag tggcggacgg cgtgccgcgg cgagcgcgac gagccgttcc
     8341 cgtacgggcc gacctacgag gacgggcggt gcaacgtgca ccgcgaggcc cacccggccg
     8401 gcgtgctcca ccgggacgtg agcctcgggc acagcgatcc gcggctcaac ctggtgcgcg
     8461 tcgccggcgc gccgctcctc cggcggacgg gcgagaccgc gacgtgcgcg agcgtgtggg
     8521 aagacgacgc ggtgagcgac atggtgggca acctggacga gtgggtcgac gatccggagg
     8581 ggacgttcgc cggcgggttc tatgcgcgcg cgacgaagca gggctgcatg gcgaccatcg
     8641 cctcgcacga cttcacctac ttcgactact cgaccggcgt ccggtgctgc gcggatctgc
     8701 gcgggccgtg atcgacgcgc cgcccagcgt gcggggcgat ctggtagcat cggccggttg
     8761 cgcgggggac gccgcgggca cgcggcgtca ggaggaggta agtcgatgat ccaggcgcgg
     8821 tattctgtct atgactcggc ggtgctggac gcccccatcg aggaggcgtg gagagagctc
     8881 cgcgatatcg tgaagctcct gccgctcgtg ttcggcgaca gcgtcaagga ctaccgttac
     8941 gtggacggcg gttcggcgga gaagatcccg tcgcggttcg agttcacgct cctgccctcg
     9001 ggggagacgg ggctcgagga ggtggtcgcg cggtccgaga ccgagcactc gctcacgtac
     9061 cggatgctcg ggcagctcat cggcgtcgag gggtacatcg cgacctaccg gctgcggcgc
     9121 atcacgaccg agccgggcaa gacgttcctg gaatggccgc gggagttcgg ggtcgcggcg
     9181 ggcgccgatc cggcagaggt cgtcccggcg atcgaggccc tcacggcgag ggaggtcgcg
     9241 accatcaagg agcatttcga gaagcggcgc cggtcgctgt aacgggggcg ccccgcgggg
     9301 tcgtcggcgc gctcaggccg tcgggcggcg cacggcgagg aagctctccg cgcccttcgg
     9361 gcgcctccgg tcgatccaga gcagcgccgc cggcagcacc agcaccgcgg cgagcaggca
     9421 ggcgagctcg ccgatcacgg cggcgacgcc gaggctgcgc acggcgtggt tctgcgagcc
     9481 gaccagggcg aggtagccga gcgtcgtcgt gaggctgcag aggatcaccg cgccgcccgt
     9541 ctcgcgcacc gcggtcagcg cgccgccggc gccctcgcgc gtgtaccgct gcacgatgtt
     9601 cacggcgtag tcgacgccga tgccgaacgt cagcggcagg gcgatgaagt tcaggaagtt
     9661 gagcttcacg tccatcgcca cgagcaggcc cgccatccag gcgacgccca ccaggagcgc
     9721 gccgagcacg gcgaacgccg ggcggccgcc gcggaaggcg ccgagcacga cgaggaccgt
     9781 cgcggcgagc gagaagagca ccgccggcgg cacgtcgtcg atcaccgcgg cccacatgtc
     9841 cgcgtagatg accgcgcgcc ccgagccgcg gacgacgctg ccgtccggga gccgcgtctc
     9901 gcggtaagcg tcggcccagc ggaagaggta ctgcgcgtcg tgcacgctct cggacgtggt
     9961 cgggctgatg tagacgatgc gcccgcgcgt cccgtccgtc tcggtgaacg cgcgcgccac
    10021 gcccgccggc agatcgccca tgccgaacgg cgcgagatcg tcgggcggga tgacggcctg
    10081 gatccgcgcc cagtcgccgt cgtcgagcag gccgcgcttc ctcgcccgga gcacgcgatc
    10141 tttgatctcg agcagcacgg ggatcttcgc cgcctggtcc cccgggacga gatcctggag
    10201 cgcgtggatc tccttgaacg gcttcgcgcc ctcgggcgcc gcgtcgcgcc gcgcgtagag
    10261 cgccgcgcgc agcggctcga cctgctcggg gcgatccacg aggatcgcca tgccgtcggc
    10321 gcccacgtag cccgtgatct cctcggccag cgcgctcagg cggatctgcg cggcgcgcgc
    10381 ctgctcgtcg ttgcggacgt tcctgaggtc gtactccatc ggatcggcgc gcacgtaggc
    10441 gaccgtggcg acgacgccgg ccgcggccag ggcgaggccc gcgaccgtga tcccgaccgg
    10501 gctcctcggc gcgatccacg cgaacggcct gccgaaggcg aggccgccct gcgtgcgccg
    10561 ccggagcctg ccgagcaggc caggcgactc ctgctcgagc gggatgagcc gctccatcac
    10621 cgcgagcacg ctcggcagcg cgacgaacgt cgcggcccag cagagcacca tgccggcgcc
    10681 gccgatcagg ccgaagtcgc ggaacccgcg gaactcggtg acgagcagcg aggcgtacgc
    10741 cgccgcggcg gcgcagccgg ccgtgagcgt gggcagccac gtctcgcggt gcgcgacgcg
    10801 cacgccctcg agcaggctcg cgccgccgcg ccgcgcctcg aggtaacgcg ccatgtagat
    10861 gatgccgaag ttgatcccgt tg
//
