LOCUS       NZ_CP012673            10870 bp    DNA     circular CON 30-MAR-2024
DEFINITION  Sorangium cellulosum strain So ce26 chromosome, complete genome.
ACCESSION   NZ_CP012673
VERSION     NZ_CP012673.1
KEYWORDS    RefSeq.
SOURCE      Sorangium cellulosum
  ORGANISM  Sorangium cellulosum
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae;
            Sorangium.
REFERENCE   1  (bases 1 to 14557589)
  AUTHORS   Zaburannyi,N., Bunk,B., Overmann,J. and Mueller,R.
  TITLE     Sorangium comparison
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14557589)
  AUTHORS   Zaburannyi,N., Bunk,B., Overmann,J. and Mueller,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-SEP-2015) Department of Microbial Natural Products
            (MINS), Helmholtz Institute for Pharmaceutical Research Saarland
            (HIPS), Campus E 8.1, Saarbruecken, Saarland 66123, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT portal v. 2.2.0, HGAP v. 3.0
            Genome Coverage                   :: 134x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002950945.1-RS_2024_03_29
            Annotation Date                   :: 03/29/2024 11:58:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10,531
            CDSs (total)                      :: 10,457
            Genes (coding)                    :: 10,397
            CDSs (with protein)               :: 10,397
            Genes (RNA)                       :: 74
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 6 of 60
            Pseudo Genes (incomplete)         :: 58 of 60
            Pseudo Genes (internal stop)      :: 2 of 60
            Pseudo Genes (multiple problems)  :: 6 of 60
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 02:39:47
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 354774
            Orig. end                         :: 365644
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP012673.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria available from German Collection of Microorganisms and
            Cell Cultures GmbH, Inhoffenstrasse 7B, 38124 Braunschweig,
            Germany.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10870
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10870
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10870
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5870](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5870
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(16..1029)
                     /locus_tag="SOCE26_RS01430"
                     /old_locus_tag="SOCE26_002850"
     CDS             complement(16..1029)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="SOCE26_RS01430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SOCE26_002850"
                     /product="hypothetical protein"
                     /protein_id="WP_104976947.1"
                     /transl_table=11
                     /translation="MPGHETGQAAPAGFSWEDYVAWLVAQHGSLTAVAERLASLRGYED
                     DVTSIERALRRLRTRGQRDGGTWGARALAAFGLPDALTERARWMGAYHSRFTDLPLPLA
                     QDLLRLWDRPPVSEDPTSHVFLALAHATCVLRGDDREAGKTHLARIRPLLGKAPPEARV
                     EALLTEAFLASRDDVARVAGLLDEIEPLFELPMPANDRACLFARWIDQRAFERNRSWGG
                     KQPDPAAAEALYRRIPEDGPAFARARRANGLSYARWKQGFPEEGAALAREACRHAGDGG
                     HMRMRAMALNMLARIVGGEEGAEARRRAMAIANELEDEVLRLRFERRAGNKGRGGL"
     gene            1077..1652
                     /locus_tag="SOCE26_RS01435"
                     /old_locus_tag="SOCE26_002860"
     CDS             1077..1652
                     /EC_number="3.1.-.-"
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /GO_function="GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233619.1"
                     /locus_tag="SOCE26_RS01435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SOCE26_002860"
                     /product="3'-5' exonuclease"
                     /protein_id="WP_104976948.1"
                     /transl_table=11
                     /translation="MGKEPAFFLVIDLEATCDEEGRIPERIMEIIEIGAVLVDGATLEP
                     VSEFATFVRPVIRPKLTEFCTKLTTITQADVDAAPTFPEAIAALGRFVGQREALFCSWG
                     DYDRKQFEIDAARWGVPLPLQGHMNIKKRFSATLGVTQRYGMASALERLGLPLHGTHHR
                     GIDDARNIARILPYAIGRVPVPDAGPAG"
     gene            complement(<1853..1996)
                     /locus_tag="SOCE26_RS56420"
                     /pseudo=""
     CDS             complement(<1853..1996)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464940.1"
                     /locus_tag="SOCE26_RS56420"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="DNA repair protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MELVLGLPGVGPRLLGTAALPSLGRLSPAALAERTQIALFDATRL
                     AAA"
     gene            complement(2013..2435)
                     /locus_tag="SOCE26_RS01445"
                     /old_locus_tag="SOCE26_002880"
     CDS             complement(2013..2435)
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /GO_function="GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233097.1"
                     /locus_tag="SOCE26_RS01445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SOCE26_002880"
                     /product="HNH endonuclease"
                     /protein_id="WP_104976949.1"
                     /transl_table=11
                     /translation="MSTYVSELLRSRIEEADRRRCQYCLTSEANSGIPLTYDHIAPVSK
                     GGPTSFENVCLACRPCNEFKAGATESVDPLTGDVVPLFNPRLHRWSDHFTWSVDATRVE
                     GVTPSGRATVIALRMNRPTIVAARRRWAEVGWHPPA"
     misc_feature    2392..2406
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.32"
                     /tool="antismash"
     gene            complement(2470..2874)
                     /locus_tag="SOCE26_RS01450"
                     /old_locus_tag="SOCE26_002890"
     CDS             complement(2470..2874)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734544.1"
                     /locus_tag="SOCE26_RS01450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SOCE26_002890"
                     /product="hypothetical protein"
                     /protein_id="WP_104976950.1"
                     /transl_table=11
                     /translation="MSTATVTLALPEALYQRLRGTALATRQPLEAVMLRALSLGSPPAW
                     DDAPPEFQVDLACLDKMDDQALWQIAKSHRSEAELVRYDELLDRNTQGLLTPAERIELE
                     RLRHESDLFMLRKAHAAALLRWRGHAVASP"
     gene            complement(3054..3680)
                     /locus_tag="SOCE26_RS56425"
     CDS             complement(3054..3680)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464940.1"
                     /locus_tag="SOCE26_RS56425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="JAB domain-containing protein"
                     /protein_id="WP_234024092.1"
                     /transl_table=11
                     /translation="MSRAPVDAPAETLPDLPTATEAALLEMVLGLPGVGPRLLGTAALP
                     SLGRLSPAALAERTQIALFDATRLAAAFELGRRYHAARARRGALLNDAARVARYFGPRL
                     ASLVHEELWIAALDTHGRVRETRLLARGSDDALYVGRATVLRTALDMAAHSFVLAHNHP
                     SGDPTPTPEDEAMTLELRHLAAELHVPFKAHVIVTPSGRYAAVHE"
     gene            complement(<3711..4400)
                     /gene="tnpB"
                     /locus_tag="SOCE26_RS56430"
                     /pseudo=""
     CDS             complement(<3711..4400)
                     /codon_start=1
                     /gene="tnpB"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /locus_tag="SOCE26_RS56430"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase.; incomplete; partial in the middle
                     of a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /pseudo=""
                     /transl_table=11
                     /translation="MSRSPPHEPGAQASAAVAELRRVVRGGLQRALAGRSGVGDALIDD
                     LTQDATLKVLGALDSFRGASRFTTWAITISIRVALTEVRKARWKDVSLDQLTPQYAELV
                     AEGAPVPAVLVEAAQHLDLCPECAEELRAAQRSGGPRSAGLRPGHARACAGAGRRGDAV
                     LTLPPSVRVYVAAEPTDLRKSFDGLSVLVAHSFGADPLCGHLFVFRNRRGDQVRILFWD
                     RTGYVIVG"
     gene            4789..4983
                     /locus_tag="SOCE26_RS01470"
                     /old_locus_tag="SOCE26_002910"
     CDS             4789..4983
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="SOCE26_RS01470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SOCE26_002910"
                     /product="hypothetical protein"
                     /protein_id="WP_104976951.1"
                     /transl_table=11
                     /translation="MQIESVQSNEPNKVEQNPFLFERVTVAKAPDEEEMLAMQPGWCAC
                     AGRCNCFCGNCAACSISPN"
     gene            5001..5870
                     /locus_tag="SOCE26_RS01475"
                     /old_locus_tag="SOCE26_002920"
     CDS             5001..5870
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /locus_tag="SOCE26_RS01475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SOCE26_002920"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_104976952.1"
                     /sec_met_domain="DUF692 (E-value: 3.5e-67, bitscore: 225.7,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRRDPLAHLRSLPYLGVGLGYRKELDASIRANRSSIDCLEIISEH
                     YFDATPDRIARLTSLAEEFPLIPHGIEMSIGTVGEVDPAYLSSLAHVTSIARGAWASDH
                     LSFTRAGGIALGQLTPLVRDCAMAIDIARKAQAVQDRLGVPFLLENVSYYVNFTSELTE
                     AEFIAEIMSRCTCGMLLDLTNLFLNSRNHGYDPLQFLETIPLDRVVQIHLAGGDSKEHQ
                     MGDVLLDLHGAAVYEESFKLLDYVAPRAANLRSVIIERDQNFPEDFGEILADLDRARQI
                     VSQVRRAA"
     gene            5867..6676
                     /locus_tag="SOCE26_RS01480"
                     /old_locus_tag="SOCE26_002930"
     CDS             5867..6676
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="SOCE26_RS01480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SOCE26_002930"
                     /product="hypothetical protein"
                     /protein_id="WP_104976953.1"
                     /transl_table=11
                     /translation="MSSSGLQAAFAHVLVDRKLRDRLSEQGEALRARFDLTGEELEKLL
                     ELSPSRLGVTATTVGAKRVDFLVRALPRTSTIFGGSCQCQLMGFVEASMPFEPSDGNQV
                     NRLVAEGQRFIEYLETRPAGAPTYAVDLANYELLHLKLNYASDAVRPPEGEVVIAGDAP
                     LQLGRHVALGEFGHDVVSLIEAPELPKAAAPAHAFVALVKSAPRKIVPYRIGPAVFRML
                     ESWRAPATLSEVRAALSAQLGPSVEPGVDAAARFALQEGILVTVAKD"
     gene            6706..8646
                     /locus_tag="SOCE26_RS01485"
                     /old_locus_tag="SOCE26_002940"
     CDS             6706..8646
                     /EC_number="2.4.-.-"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /locus_tag="SOCE26_RS01485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SOCE26_002940"
                     /product="glycosyltransferase"
                     /protein_id="WP_159396553.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 2.4e-12,
                     bitscore: 46.1, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKYPPIQGGVSARCYWIARGLAKRGHQVTVVTNATEVEDDYRIWT
                     SPEDIARLEAEFDNGGRVRVVTTGHFRQSSWYIPYANPYLTKLAALGTEEIRRIDAEVV
                     FSSYFEPYGMSAHLAASWTGIPHVVQHAGSDRTRLMDHPELSLAYREMLRRASLVVTGD
                     LDLSGLGIPLDRFARIRHGHLPDEFSPEGPKLDIDALVRRAAEPGAPGVNNTRPIDRDA
                     VTIGLYGKLGEVKGSYDLIAALARLRKQGKRFNLVTLSNGREKTKFLAAIREAGIDDCT
                     WVLPFIPHWRVPEFIRACTAVCFLERKFPVEIHTPSLPREVMACGTCLILSGEIANKPY
                     CSPGLRDGENVLVVQDPSNIDQLAATLDQVITDPERARAIGMAGIGVYSHKPIDDLGET
                     YEELFARAIKNGASPAKMDLGVAKHTWEEEILSMLRRFMPVSFRGLEARIRASLRGFLE
                     SFDLSGASMESAALAFADELLARDEWPSELAPAAVTREVFRFEREQLWLVVDVETKRGE
                     APFPEHRRPHAEAGVTKDGRRVLGALKPVRSAWVHVEQFAVDVEAAIATLVTGQKITSM
                     PTLAEPALYVFQKRGSLKGRVFRINREARDFLAGCDEKRTVAELVGAGISRDHLYDFVR
                     QLAKEQVIALR"
     gene            8675..10408
                     /locus_tag="SOCE26_RS01490"
                     /old_locus_tag="SOCE26_002950"
     CDS             8675..10408
                     /GO_function="GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA]"
                     /GO_process="GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GATase_7"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /locus_tag="SOCE26_RS01490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SOCE26_002950"
                     /product="asparagine synthase-related protein"
                     /protein_id="WP_159396554.1"
                     /sec_met_domain="GATase_7 (E-value: 1.8e-28, bitscore:
                     98.2, seeds: 307, tool: rule-based-clusters)"
                     /sec_met_domain="Asn_synthase (E-value: 2.8e-31, bitscore:
                     108.7, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MCGIVAVVGQRAEDKVLSAMLDAVEARGPDGRESVSGDWFSVGFC
                     RLAIYALEAGRQPVRSPDGAVTLAFNGEIYNFEALAERLGIRAASEAELLLQGYLAHGS
                     SFVSQLDGDFAVVIVDEKRRVCELWRDRFGVKPLYYTMLPGRRLLISSEIRGLLAHPDF
                     VLGWDELALAERYVLTFWSTERTTFKGVRQILPGHRTVIGPFDGSGAEGGALHVEEIPY
                     TPERQISVVGECEVVKACTDTLQASIAKRVQHTQVSPVVVALSGGIDSSLLAALARRAS
                     SELAAITICGGPDELDARHSTLLASKLGIEHHLYTVSTERLLEVIPAVVLAQPANGAFL
                     AWFLAEAMQRLTPSAKVLLCGEGADEMFHGYRVHSDPAGFKAQTLDALSAVSDLEGGSA
                     LLRRVRGWEQMEPAAYYEDVTALFLSAQLVNRHLVPFDHGTMARRVECRVPFLGLEMLD
                     LHDRTPRRALGPNKPLLRRALNQLPELDASTRDALLTRQRTAGYTATEAARAGLRRLLM
                     DRGLTRSRLMASKIMRFSRNPEELFWLGAAHVVYFERRGKIDGMDFETLLEEAFNASPE
                     W"
     misc_feature    complement(10551..10565)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.92"
                     /tool="antismash"
ORIGIN
        1 aggatccgag ctggctcaaa gaccaccccg gcccttgttt cccgctcgcc gctcgaaccg
       61 caacctgagc acctcgtcct cgagctcgtt cgcgatcgcc atcgcccgcc gcctcgcctc
      121 cgcgccctcc tcgccgccca cgattcgcgc cagcatgttc agcgccatcg cgcgcatccg
      181 catgtgcccg ccgtcccccg cgtgccggca cgcctcccgc gcgagcgccg cgccttcctc
      241 ggggaaaccc tgcttccacc gcgcatacga aaggccgttc gcccgccgcg cccgcgcgaa
      301 ggccggaccg tcctccggga tgcgccggta aagcgcctcc gcagccgccg gatccggctg
      361 cttgccgccc caggagcggt tccgctcgaa ggcgcgttga tcgatccacc gggcgaacag
      421 gcaggctcgg tcgttggcgg gcatcggcag ctcgaagagc ggctcgatct cgtcgagcag
      481 gccggccacg cgcgcgacat catcacggct cgccaggaag gcctccgtga gcagagcctc
      541 cacccgcgcc tcgggcgggg cctttccgag cagcggccgg atgcgggcga ggtgcgtctt
      601 tccggcctcg cggtcgtcgc cgcgcagcac gcaggtggcg tgggcgaggg cgaggaagac
      661 gtgggaggtc ggatcctcgc tcaccggcgg tcgatcccag aggcgcaaga ggtcctgggc
      721 gaggggcagg ggcaggtcgg tgaaccgcga gtgataggcg cccatccagc gggcgcgctc
      781 ggtgagcgcg tccggcaagc cgaaggccgc gagcgcgcgt gccccccagg tcccgccgtc
      841 ccgctggcca cgcgtccgca gccggcgcag ggcgcgctcg atcgaggtga cgtcgtcctc
      901 gtagccacgc agggaggcga gccgctcggc gaccgcggtc aaggacccgt gctgcgcgac
      961 gagccaggcg acgtaatcct cccaggaaaa gcctgcggga gcggcctgtc cggtttcgtg
     1021 tccgggcatg ttcttcgcgc ggatcgtatc aaggttcagc agaaaggagg acggacatgg
     1081 gaaaggaacc tgcatttttc ctggtgatcg atctggaggc gacgtgcgac gaggagggcc
     1141 gcatccccga gcgcatcatg gagatcatcg agatcggcgc ggtcctcgtg gacggcgcga
     1201 cgctcgagcc cgtgagcgag ttcgccacct tcgtgcgccc ggtcatccgg ccgaagctca
     1261 cggagttctg cacgaagctc accacgatca cgcaagcaga cgtcgacgcc gcgcccacgt
     1321 tccccgaagc catcgcggcg ctggggcgct tcgtcggcca gcgggaggcg ctcttctgct
     1381 cgtggggcga ttacgaccgc aagcagttcg agatcgacgc ggcccgctgg ggcgtgcccc
     1441 tgccgctgca ggggcacatg aacatcaaga agcgcttctc cgcgacgctc ggggtgaccc
     1501 agcgctacgg catggcgagc gcgctcgagc ggctcggact cccactccac gggacacacc
     1561 accgcgggat cgacgatgcg cgcaacatcg cacgaatcct gccttacgcg atcggccgcg
     1621 tgccggtccc ggatgcaggg cccgcggggt gagcgcctcg ctggggacca cgagggcgcg
     1681 gtcgcggccg gggcgagcgc ctgtcccgcg ccgtccgagg tcgtgcgcgc cggggtcacg
     1741 atgccgcgcc ccggtgggga cgccggcgtg cgccggcggt cgctggagtc gccgtgtgga
     1801 ggcgggtcga ccgcgatgcg ttacgctggg tcgcgctcag cgagggccta caggcggcgg
     1861 cgagccgggt cgcgtcgaag agggcgatct gggtgcgctc ggccagcgcc gcgggagaga
     1921 ggcgaccaag cgaggggaga gcggccgtgc cgagcaggcg cggcccgacg ccgggcagac
     1981 cgagcaccag ctcgatgaag gggtagccag cactacgcgg gcgggtgcca gcccacctcg
     2041 gcccagcgcc gacgtgcggc gacgatcgtc gggcggttca tccggagcgc gatgaccgtt
     2101 gctctaccgg acggcgtcac gccctctacc ctggttgcgt cgacgctcca cgtgaagtga
     2161 tcgctccagc gatgaaggcg cggattgaag agcggcacga cgtctcccgt cagcgggtcg
     2221 accgactccg tggcgcctgc cttgaactcg ttgcatgggc ggcatgcgag gcatacattc
     2281 tcgaacgagg tcggcccgcc cttcgagacc ggcgcgatgt gatcgtaggt caggggaatt
     2341 ccgctattgg cctcgcttgt caggcagtat tgacagcggc gccggtcggc ctcctcgata
     2401 cgagaacgga gcagttcgga gacgtaggtt gacaccgcag tccgtcttcc atctcgtgga
     2461 gggcgcgcgc tacggagaag cgacggcgtg cccgcgccat cgcaggagcg ccgctgcgtg
     2521 cgccttgcgg agcatgaaca ggtcgctctc gtggcgaaga cgctcgagct cgatgcgctc
     2581 cgccggcgtg aggagcccct gggtgttccg gtcgagcagc tcgtcgtagc gcacgagctc
     2641 tgcttcggac ctgtggctct ttgcgatctg ccagagcgcc tggtcgtcca tcttgtccag
     2701 gcaggcgagg tcgacctgga actccggggg cgcgtcgtcc caggcgggag ggctaccgag
     2761 gctcagcgcc cgcagcatca cggcttcgag cggctgtcgg gtcgcgagcg cagtgcccct
     2821 cagtcgctgg tagagcgctt cggggagagc gagggtgacg gtggcggtcg acatcgcgct
     2881 ggggtggacg cgggaggcaa gcccgcatcg ccgtcgagcc tagcacgagg agcaccccgg
     2941 ctcctccccg gccagccgcg atgacgagag cgaacgcgcg gcgggctgcc ggctggggcg
     3001 gtgcgcggga ggctttcggc tggatgcgca ggggcggtgc gccccgcgcg ggcctactcg
     3061 tggacggcgg cgtagcggcc gcttggggtg acgatgacgt gggccttgaa cggaacgtgc
     3121 agctccgcgg cgaggtggcg cagctccagg gtcatcgctt cgtcctcggg ggtcggcgtc
     3181 gggtcgccgc tcgggtggtt atgggccagc acgaagctgt gggcggccat gtccagcgcc
     3241 gtgcgcagga cggtggcgcg gccgacgtag agggcgtcgt cggaaccgcg ggcgagcagg
     3301 cgcgtctcgc ggacgcggcc gtgggtgtcg agcgcggcga tccagagctc ctcgtggacc
     3361 agggaggcga gcctcgggcc gaagtaccgc gccacgcggg cggcgtcgtt gaggagcgcg
     3421 ccccggcgtg cgcgggcggc gtggtagcgg cggccgagtt cgaaggcggc ggcgagccgg
     3481 gtcgcgtcga acagcgcgat ctgggtgcgc tcggcgaggg cggcgggcga gaggcgaccg
     3541 agcgagggga gagcggccgt gccgaggagg cgcgggccga cgccgggcag gccgagcacc
     3601 atctcgagga gggcggcctc ggtcgcggtc gggagatcgg gcagcgtctc ggcgggggcg
     3661 tcgacggggg cgcggctcat cgggctcatc ggggccaccg ccagcttctt tcccacgatt
     3721 acgtagccag tgcggtccca gaacaagatc cggacctggt cgcctcgccg attcctgaac
     3781 acgaacagat gcccgcagag cgggtcggct ccgaagctgt gcgcgaccag caccgacaac
     3841 ccatcgaagc tcttgcgcag gtcggtaggc tcggccgcga cgtagacgcg caccgacggc
     3901 ggcagcgtca gcacggcgtc tcctcggcgg ccagcgccag cacacgctcg agcgtggccg
     3961 ggtcgaagcc cggccgaacg cggaccaccc gaccgttggg cagcgcgcag ctcctcggcg
     4021 cactcggggc acaggtcgag gtgctgcgcg gcctcgacca gcaccgccgg caccggggcg
     4081 ccctccgcga ccagctcggc gtactgcggc gtgagctgat ccagcgacac gtccttccag
     4141 cgggccttcc gcacctccgt gagcgccacg cggatggaga tggtgatcgc ccaggtggtg
     4201 aatcggctgg cgccccggaa ggaatcgagc gcgccgagca ccttcagcgt ggcgtcctga
     4261 gtcaggtcgt cgatcagggc gtcccccacg ccgctgcgtc cggcgagcgc ccgctgcagg
     4321 cctccccgca cgacgcgtcg cagctcggcg acagcggcgc tcgcctgcgc tccgggctcg
     4381 tgaggaggag accggctcat cccgtcgacc agatcggctc acacgtcgcg gtagcagcgc
     4441 tcacgctcct cgacatctct cagcccgcgg ccggcgaccc atcggacggc accacgcctc
     4501 ccggttgcgc cagtcgcgcg caagccgcgc ctcaccgcct gcaccagttg agcaaggtcg
     4561 gcgcaacccg ctccgcgctc gcgcaaatct cccggaaacc acgcggaaat acggcgtcag
     4621 cggcctggca tccccggtgc tttggggttg agagcgaacg ccatcacgac gtcccacgga
     4681 cgtcatgacc gttcgccggc ggccccaggg acggctctgt cccgaagccc gccggtcccc
     4741 cttgcgtgag gcttgtgtcc acacacggac acagaaccgg agaagaccat gcagatcgag
     4801 agcgtgcagt cgaacgagcc gaacaaggtc gagcagaacc cgttcctgtt cgagcgggtc
     4861 accgtcgcca aggccccgga cgaggaggag atgctcgcga tgcagccggg ctggtgtgct
     4921 tgcgcgggcc gctgcaattg cttctgtggc aattgcgccg cgtgctccat ctcccccaac
     4981 tgagagccta ggtgcacgtc atgcgacgcg atcctcttgc tcacctccgc tcgctgccat
     5041 acctcggcgt cggcctcggc tatcgcaagg agctggacgc cagcatccgg gccaaccgat
     5101 cgtcgatcga ctgtctggag atcatctccg agcactactt cgacgcgacc ccggaccgga
     5161 tcgcgcgcct gacgtcgctc gccgaggagt ttccgctcat cccccacggt atcgagatgt
     5221 ccatcggtac cgtgggggag gtggatccgg cctacctgag ctccctcgcc cacgtcacct
     5281 cgatcgcgcg gggcgcctgg gcgagcgatc acctctcgtt cacccgcgcc ggcggcatcg
     5341 ccctcgggca gctcacgccc ctggtccgcg attgcgccat ggccatcgac atcgcgcgaa
     5401 aggcgcaggc cgtgcaggac cggctcggcg tccccttcct gctggagaac gtctcgtatt
     5461 acgtgaactt cacctcggag ctcaccgagg cggagttcat cgccgagatc atgtcgcgct
     5521 gcacctgcgg catgctcctc gacctcacca acctcttctt gaactcgagg aaccacggct
     5581 acgaccccct ccagttcctc gagacgatcc cgctcgaccg cgtcgtccag atccacctcg
     5641 ccggcggcga ctccaaggag caccagatgg gcgacgtcct gctcgatctc cacggcgccg
     5701 cggtctacga ggagtcgttc aagctcctcg attacgtggc cccgcgggcc gcgaacctcc
     5761 gctcggtcat catcgagcgg gaccagaact tccccgagga cttcggcgag atcctcgccg
     5821 acctcgatcg ggcgcggcag atcgtgtcgc aggtgcggag ggccgcatga gcagcagcgg
     5881 actccaggcg gcgttcgccc acgtgctcgt cgatcgcaag ctccgcgatc ggctgagcga
     5941 gcagggcgag gcgctgcggg ccaggttcga tctcacgggc gaggagctag agaagctcct
     6001 cgagctcagc ccctcgcggc tcggggtgac ggcgacgacg gtgggcgcca agcgggtgga
     6061 cttcctcgtg cgcgcgctcc cccggacgag cacgatcttc ggcggaagct gtcagtgcca
     6121 gctgatgggc ttcgtcgagg cctcgatgcc gttcgagccc tcggacggca accaggtcaa
     6181 ccggctcgtc gccgagggcc agcggttcat cgagtacctc gagacccggc cggcgggcgc
     6241 gcccacgtac gcggtggatc tggcgaacta cgagctcctg cacctgaagc tcaactacgc
     6301 ttcggacgcc gtccgccccc cggagggaga ggtggtgatc gccggcgacg ccccgctcca
     6361 gctcggccgt cacgtggcgc tcggggagtt cggccacgac gtggtctcgc tgatcgaggc
     6421 cccggagctg ccgaaggccg ccgccccggc ccacgcgttc gtcgcgctcg tgaaatcagc
     6481 cccgcggaag atcgtgccgt accgcatcgg tccggcggtg ttccgcatgc tggagtcctg
     6541 gagggctcca gcgaccttga gcgaagtgcg cgcggccctg tccgcgcagc tcgggccgag
     6601 cgtcgagccc ggcgtcgacg ccgccgcccg cttcgccctc caggaaggca ttctcgtcac
     6661 cgtggccaag gattgagagg ttcgaacatg cgcatctgtt gcatcatgaa gtatcccccc
     6721 atccagggtg gagtcagcgc tcgttgctac tggatcgcgc ggggcctcgc caagcggggg
     6781 caccaggtca cggtcgtgac gaacgccacc gaggtcgagg acgattaccg catctggacc
     6841 tcgcccgagg acatcgcccg cctggaggcc gagttcgaca acggcggtcg ggtgcgggtc
     6901 gtcaccacgg ggcatttccg gcagagctcc tggtacatcc cctacgccaa cccgtacctc
     6961 accaagctcg ccgcgctcgg caccgaggag atccggcgca tcgacgccga ggtggtgttc
     7021 tcctcgtact tcgagcccta cgggatgagc gcccacctgg ccgcctcctg gaccggcatc
     7081 ccgcacgtgg tgcagcacgc cggcagcgat cgcacccggc tcatggatca ccccgagctc
     7141 tccctcgcct accgggagat gctgcgccgc gcctccctgg tggtgaccgg cgatctcgat
     7201 ctcagcggcc tcggcatccc gctggatcgc ttcgcccgca tccgccacgg ccacctcccc
     7261 gacgagttca gcccggaggg tccgaagctc gacatcgacg cgctggtccg ccgcgccgcc
     7321 gagccgggcg cgcccggggt caacaacacg cgccccatcg accgcgacgc ggtgaccatc
     7381 ggcctctatg gcaagctcgg cgaggtcaag ggctcgtacg atctcatcgc cgccctcgcc
     7441 cgcctgcgca agcagggcaa gcgcttcaac ctggtcacgc tctccaacgg ccgggagaag
     7501 accaagttcc tcgccgccat ccgcgaggcc gggatcgacg actgcacctg ggtgctgccg
     7561 ttcatcccgc actggcgcgt ccccgagttc atccgcgcct gcaccgcggt gtgcttcctg
     7621 gagcgcaagt tcccggtgga gatccacacg cccagcctcc cgcgcgaggt catggcctgc
     7681 ggcacctgcc tgatcctctc cggggagatc gccaacaagc cttattgctc gccggggctg
     7741 cgcgacggcg agaacgtgct cgtggtccag gatccgtcga acatcgacca gctcgcggcg
     7801 acgctcgacc aggtgatcac cgatccggag cgggcccgcg ccatcggcat ggccgggatc
     7861 ggcgtctact cccacaagcc gatcgacgat ctcggcgaga cctacgagga gctgttcgcc
     7921 cgcgcgatca agaacggcgc ctcgccggcg aagatggacc tcggcgtcgc caagcacacc
     7981 tgggaggagg agatcctctc gatgctgcgc cgcttcatgc cggtgtcgtt ccgcggcctc
     8041 gaggcgagaa tccgcgcctc gctgcgcggc ttcctggaga gcttcgacct ctccggcgcc
     8101 agcatggagt cggcggcgct ggccttcgcc gacgagctcc tcgcccgcga cgagtggccg
     8161 agcgagctcg cccccgccgc ggtgacgcgc gaggtcttcc gcttcgagcg cgagcagctc
     8221 tggctggtgg tcgacgtgga gaccaagcgc ggcgaggcgc ccttccccga gcaccgccgc
     8281 ccccacgccg aggcgggcgt gaccaaggac ggccgccgcg tcctcggagc cctcaagccg
     8341 gtccgcagcg cctgggtgca cgtcgagcag ttcgccgtcg acgtcgaggc ggcgatcgcc
     8401 accctggtca ccggccagaa gatcacgtcg atgcccaccc tggccgagcc cgcgctctac
     8461 gtcttccaga agcgcggcag cctcaagggc cgtgtcttcc gcatcaaccg cgaggcccgg
     8521 gatttcctcg ccggctgcga cgagaagagg acggtggccg agctcgtggg cgcgggcata
     8581 agccgcgatc acctctacga cttcgtccgc cagctcgcca aggagcaggt gatcgccctc
     8641 cgctagatcg acgcatcgac caaggagctc gaccgtgtgt ggaatcgtcg cggtggtcgg
     8701 ccagcgggcc gaagacaagg tcctctcggc catgctggac gccgtggagg cgcgcggccc
     8761 ggacggtcga gagagcgtat cgggtgactg gttctccgtc gggttctgcc ggctggcgat
     8821 ctatgccctg gaggcaggtc gccagccggt acggagcccc gacggggccg tgaccctcgc
     8881 cttcaacggc gagatctaca atttcgaggc cctggcggag cggctcggga tccgggccgc
     8941 ctccgaggcc gagctgctgc tccagggcta cctggcccac ggctcgagct tcgtgtctca
     9001 gctcgacggc gacttcgccg tggtgatcgt cgacgagaag cgccgcgtct gcgagctgtg
     9061 gcgcgatcgc ttcggggtga agccgctcta ttataccatg ctccccggcc gccgcctcct
     9121 gatctcctcc gagatccgcg gcctcctcgc ccaccccgac ttcgtgctgg gctgggacga
     9181 gctggcgctg gcggagcgct acgtgctgac cttctggtcc accgagcgga ccacgttcaa
     9241 gggcgtgcgc cagatcctcc cggggcaccg gaccgtgatt ggccctttcg atggctccgg
     9301 tgcagagggc ggcgcgctgc acgtggaaga gatcccctat accccggagc ggcagatctc
     9361 ggtcgtcgga gagtgcgagg tcgtcaaggc gtgcaccgac accctgcagg cttcgatcgc
     9421 caagcgggtc cagcacacgc aggtgtcgcc ggtggtcgtc gcgctctccg ggggcatcga
     9481 ttccagcctg ctcgccgcgc tggcgcgccg cgcctcgagc gagctcgccg ccatcaccat
     9541 ctgcggcggg cccgatgagc tcgatgcgcg ccattcgacc ctgctcgcct cgaagctcgg
     9601 catcgagcac cacctctaca cggtgagcac cgagcggctg ctcgaggtca tccccgcggt
     9661 ggtgctggcc cagccggcca acggcgcctt cctcgcctgg ttcctcgccg aggcgatgca
     9721 gcgcctcact ccgagcgcca aggtcctgct ctgcggcgag ggcgccgacg agatgttcca
     9781 tggctatcgc gtccacagcg atcccgccgg cttcaaggcg cagacgctgg acgcgctctc
     9841 cgcggtgagt gatctcgagg gcggctccgc gctgctccgc cgggtgcggg gctgggagca
     9901 gatggagccc gccgcctact acgaggacgt cacggccctg ttcctgagcg cccagctggt
     9961 gaaccgccac ctggtgccgt tcgatcacgg caccatggcg cggagggtgg agtgccgcgt
    10021 ccccttcctc ggcctggaga tgctcgacct tcacgaccgc accccgcgcc gcgccctcgg
    10081 ccccaacaag cctctcctcc ggcgagctct caaccagctc cccgagctcg acgcctcgac
    10141 ccgcgacgcg ctcctcaccc ggcagcggac cgccggctac acggcgaccg aggcggcgcg
    10201 cgccggcctg cgcaggctgc tgatggaccg cgggctgacc cgctcgcggc tgatggcctc
    10261 gaaaatcatg cgcttctccc gcaaccccga ggagctgttc tggctgggcg ccgcgcacgt
    10321 cgtctatttc gagcgccgcg gcaagatcga cgggatggat ttcgagacct tgctagagga
    10381 agccttcaac gcctctcccg agtggtaggg ccccgtgaag ctcgacgacc tctgcgccac
    10441 cttccagtcc ctcgcatttc cacgcctcgg cgagcgcgcg cacccggagc gccaggcggt
    10501 gctcgacgcc cgcgcccagg tcttcaccgc cgtggccgac gacgagttcc tggccgattg
    10561 catcgatcgg gagctgcgcc tgatcgagga gggtcggctt cgacgcgggc tctcgccctt
    10621 cttcaccctg ccggagctgg ggatccactt caccttcggc tactggtccc ccgggatgac
    10681 gccgggcccg cacgagcaca ccgcctggac catcaccgcg gtctgccgca actggctcga
    10741 ggtcctgacc tacaaccgcg aggagtccta ccggcgccgc gggctggtgc ccaagaatcg
    10801 cttcgaggcg cccgcgggaa aggtcggcta catctacgat ccttgcatcc acgagcccag
    10861 gaacacctcg
//
