LOCUS       NZ_POVS01000042         2137 bp    DNA     linear   CON 15-SEP-2024
DEFINITION  Paenibacillus sp. F4 F4_contig_41, whole genome shotgun sequence.
ACCESSION   NZ_POVS01000042
VERSION     NZ_POVS01000042.1
KEYWORDS    WGS; RefSeq.
SOURCE      Paenibacillus sp. F4
  ORGANISM  Paenibacillus sp. F4
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Jeong,H., Park,S.-Y., Choi,S.-K., Ryu,C.-M. and Park,S.-H.
  TITLE     Genome sequencing and comparative analysis of polymyxin-producing
            Paenibacillus strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Jeong,H., Park,S.-Y., Choi,S.-K., Ryu,C.-M. and Park,S.-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JAN-2018) Infectious Disease Research Center, Korea
            Research Institute of Bioscience and Biotechnology (KRIBB), 125
            Gwahak-ro, Daejeon 34141, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 11-JAN-2018
            Assembly Method                   :: CLC Genomics Workbench v. 11.0
            Assembly Name                     :: F4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 524.4x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002894905.1-RS_2024_09_15
            Annotation Date                   :: 09/15/2024 04:37:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,419
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,203
            CDSs (with protein)               :: 5,203
            Genes (RNA)                       :: 120
            rRNAs                             :: 10, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 10, 1 (5S, 16S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 102
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 42 of 96
            Pseudo Genes (incomplete)         :: 67 of 96
            Pseudo Genes (internal stop)      :: 14 of 96
            Pseudo Genes (multiple problems)  :: 22 of 96
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 02:44:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 2137
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            POVS01000042.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     protocluster    1..2137
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[485:>2137](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      486..>2137
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     gene            <1..>204
                     /locus_tag="C1T21_RS26900"
                     /old_locus_tag="C1T21_26905"
     CDS             1..>204
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017427039.1"
                     /locus_tag="C1T21_RS26900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C1T21_26905"
                     /product="hypothetical protein"
                     /protein_id="WP_146032532.1"
                     /transl_table=11
                     /translation="MQGQNQLVAYYVAEQEMNAGVLRSLLGEKLPHYMVPSYFVQLESM
                     PLTPNGKIDRKALPAPEGSLQTG"
     source          1..2137
                     /collected_by="Seung-Hwan Park (KRIBB)"
                     /db_xref="taxon:357385"
                     /mol_type="genomic DNA"
                     /organism="Paenibacillus sp. F4"
                     /strain="F4"
                     /submitter_seqid="F4_contig_41"
     region          1..2137
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..2137
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     assembly_gap    205..483
                     /estimated_length=279
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <484..>2137
                     /locus_tag="C1T21_RS26905"
                     /old_locus_tag="C1T21_26910"
     CDS             486..>2137
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Condensation_LCL (1-314). E-value:
                     3.3e-90. Score: 294.0. Matches aSDomain:
                     nrpspksdomains_C1T21_RS26905_Condensation_LCL.1"
                     /codon_start=2
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014281409.1"
                     /locus_tag="C1T21_RS26905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C1T21_26910"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_146032533.1"
                     /sec_met_domain="Condensation (E-value: 1.2e-57, bitscore:
                     182.3, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 1.3e-17, bitscore:
                     50.5, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLHQIDPDDVNYNIPAVLQVSGPLDVKRVKKVFRQLISRHAAFRT
                     SFGLVNNEPVQRIQETVFFEVEYAKLQGGRVLTHIEADQQIHVPISDRDTAFEQQTVGS
                     EQHVSREVQDWVQRFVRPFDLQVAPLLRVSLLDLGMQGGEQEQQYLMMLDMHHIISDGV
                     STGVLTKEFVRLYSGEVLPPLRIQYKDYAIWQQGETQQDWMKRQEMFWLDNFRGELPLL
                     DLPTDFTRPAVRSTAGDMVVFGLEREVSERLKELAAQTGSTLYMVLLAAYTALLHHYTG
                     QEDIIIGTPIAGRRYADLEPIIGMFVGTLAIRNYPTAAQTFRHYVEDVKKRALQAYEHQ
                     DYPFEELVEKLNVKRDMSRNPLFDTMFVLQNTEQDELKLPDINFRPFGMEQVPAKFDLT
                     LEASEQEDGIQFGLQYATTLYKRETIEQITRHFIRLTEIITTNPDTKLGELDLLSEQER
                     EQILHVWGDTAAAYPSEQTIHGLFEAQALQTPEQAALFFEGEQLTYRELNERANRLART
                     LRSHGVTKDRLVGLMTERSVDMIVGMFGIMKAGG"
     aSDomain        490..1428
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_C1T21_RS26905_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="3.30E-90"
                     /label="C1T21_RS26905_Condensation_LCL.1"
                     /locus_tag="C1T21_RS26905"
                     /protein_end="314"
                     /protein_start="1"
                     /score="294.0"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIISDG"
                     /tool="antismash"
                     /translation="LHQIDPDDVNYNIPAVLQVSGPLDVKRVKKVFRQLISRHAAFRTS
                     FGLVNNEPVQRIQETVFFEVEYAKLQGGRVLTHIEADQQIHVPISDRDTAFEQQTVGSE
                     QHVSREVQDWVQRFVRPFDLQVAPLLRVSLLDLGMQGGEQEQQYLMMLDMHHIISDGVS
                     TGVLTKEFVRLYSGEVLPPLRIQYKDYAIWQQGETQQDWMKRQEMFWLDNFRGELPLLD
                     LPTDFTRPAVRSTAGDMVVFGLEREVSERLKELAAQTGSTLYMVLLAAYTALLHHYTGQ
                     EDIIIGTPIAGRRYADLEPIIGMFVGTLAIRN"
     CDS_motif       520..627
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C1T21_RS26905_0001"
                     /evalue="7.10E-14"
                     /label="C2_LCL_024-062"
                     /locus_tag="C1T21_RS26905"
                     /protein_end="47"
                     /protein_start="11"
                     /score="44.1"
                     /tool="antismash"
                     /translation="YNIPAVLQVSGPLDVKRVKKVFRQLISRHAAFRTSF"
     CDS_motif       949..1014
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C1T21_RS26905_0002"
                     /evalue="4.10E-10"
                     /label="C3_LCL_132-143"
                     /locus_tag="C1T21_RS26905"
                     /protein_end="176"
                     /protein_start="154"
                     /score="32.1"
                     /tool="antismash"
                     /translation="MHHIISDGVSTGVLTKEFVRLY"
     CDS_motif       1045..1074
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C1T21_RS26905_0003"
                     /evalue="2.70E-05"
                     /label="C4_LCL_164-176"
                     /locus_tag="C1T21_RS26905"
                     /protein_end="196"
                     /protein_start="186"
                     /score="16.7"
                     /tool="antismash"
                     /translation="QYKDYAIWQQ"
     CDS_motif       1336..1425
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C1T21_RS26905_0004"
                     /evalue="3.90E-13"
                     /label="C5_LCL_267-296"
                     /locus_tag="C1T21_RS26905"
                     /protein_end="313"
                     /protein_start="283"
                     /score="41.7"
                     /tool="antismash"
                     /translation="DIIIGTPIAGRRYADLEPIIGMFVGTLAIR"
     CDS_motif       1474..1593
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C1T21_RS26905_0005"
                     /evalue="7.50E-23"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="C1T21_RS26905"
                     /protein_end="369"
                     /protein_start="329"
                     /score="73.0"
                     /tool="antismash"
                     /translation="KKRALQAYEHQDYPFEELVEKLNVKRDMSRNPLFDTMFVL"
ORIGIN
        1 gggcaagggc aaaaccaact ggtagcctac tatgtggcag aacaagaaat gaatgcaggt
       61 gtgctgcgca gcctactggg tgaaaagctg cctcactata tggtaccttc atactttgta
      121 cagctggagt ccatgccgtt aacaccgaac ggcaaaatag accgcaaggc attgccagca
      181 cccgaaggca gcctgcaaac gggannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnncgttttg catcagattg atccagatga tgtgaactac aatattcccg ctgtgcttca
      541 ggtaagtggt ccgctggatg taaaacgggt gaagaaggtt ttccgccagt tgatctcacg
      601 tcatgcagct ttccgcacca gctttggact ggtaaacaat gaacccgtgc aacggattca
      661 agaaactgta ttcttcgagg tggagtatgc gaagttgcag ggagggcgtg tactaacgca
      721 catagaagct gatcaacaaa tacatgtgcc catctctgat cgtgataccg cgttcgagca
      781 gcaaacagta gggtctgaac agcatgtaag tcgagaagtt caagattggg ttcaacgttt
      841 tgtacgtccg tttgatctgc aggtagcccc gttgctacgg gtatccttgt tggatctggg
      901 tatgcagggg ggagaacaag aacagcaata tctgatgatg ctcgatatgc accatattat
      961 ctcggacggt gtatcaacag gtgtgttaac caaagaattt gtacgcttat acagcggtga
     1021 ggtattgcct ccgctacgga ttcaatacaa agactatgcc atatggcagc aaggtgaaac
     1081 acagcaggat tggatgaaac gtcaggagat gttctggtta gataacttca ggggtgaact
     1141 gcccttactg gatcttccaa ccgactttac tcgcccggca gtgcggagca cggctggaga
     1201 tatggtggta tttggactgg aacgtgaagt cagcgaacga ttaaaagagc tggcagccca
     1261 gacaggttcg acgctgtata tggtgcttct ggcggcctat acagcacttc tgcatcatta
     1321 cactggacag gaagacatca ttatcggtac cccgattgcg ggccgacgtt atgccgattt
     1381 ggagccaatt atcggaatgt ttgtcggaac actagccata cgcaactacc caaccgcagc
     1441 gcaaacattc cgccactatg tggaggatgt gaaaaaacgg gccttgcaag cgtatgagca
     1501 tcaggactat ccatttgagg aattggtgga aaaactgaat gtgaaacggg atatgagccg
     1561 taacccactg ttcgacacga tgtttgtgct acaaaacacg gaacaagacg agttgaaact
     1621 tccagatatt aacttccggc catttggtat ggagcaggtt ccagctaagt ttgacttgac
     1681 gctggaagcc agcgaacaag aggatggcat tcaattcggt ttgcagtatg cgacaacatt
     1741 gtataaacgg gaaacgattg agcaaatcac acgccacttt atacgactga ctgaaatcat
     1801 tacgacgaat cccgatacga aattgggtga gctggattta ttgagtgagc aggaacgcga
     1861 gcaaatcctg cacgtgtggg gagatacggc ggcagcgtat ccaagcgagc agacaattca
     1921 cggtttgttt gaagcgcagg ctttgcaaac accagagcag gcggccttgt tcttcgaggg
     1981 agaacagctg acctaccgcg agctgaatga acgagccaac cgattggctc gtaccttacg
     2041 gagtcatggc gtgacgaagg atcgtctggt gggtctgatg acggagcgct cggtagatat
     2101 gatcgtgggg atgttcggga ttatgaaagc cggaggc
//
