LOCUS       NZ_POVS01000031         2085 bp    DNA     linear   CON 15-SEP-2024
DEFINITION  Paenibacillus sp. F4 F4_contig_30, whole genome shotgun sequence.
ACCESSION   NZ_POVS01000031
VERSION     NZ_POVS01000031.1
KEYWORDS    WGS; RefSeq.
SOURCE      Paenibacillus sp. F4
  ORGANISM  Paenibacillus sp. F4
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Jeong,H., Park,S.-Y., Choi,S.-K., Ryu,C.-M. and Park,S.-H.
  TITLE     Genome sequencing and comparative analysis of polymyxin-producing
            Paenibacillus strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Jeong,H., Park,S.-Y., Choi,S.-K., Ryu,C.-M. and Park,S.-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JAN-2018) Infectious Disease Research Center, Korea
            Research Institute of Bioscience and Biotechnology (KRIBB), 125
            Gwahak-ro, Daejeon 34141, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 11-JAN-2018
            Assembly Method                   :: CLC Genomics Workbench v. 11.0
            Assembly Name                     :: F4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 524.4x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002894905.1-RS_2024_09_15
            Annotation Date                   :: 09/15/2024 04:37:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,419
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,203
            CDSs (with protein)               :: 5,203
            Genes (RNA)                       :: 120
            rRNAs                             :: 10, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 10, 1 (5S, 16S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 102
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 42 of 96
            Pseudo Genes (incomplete)         :: 67 of 96
            Pseudo Genes (internal stop)      :: 14 of 96
            Pseudo Genes (multiple problems)  :: 22 of 96
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 02:44:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 2085
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            POVS01000031.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2085
                     /collected_by="Seung-Hwan Park (KRIBB)"
                     /db_xref="taxon:357385"
                     /mol_type="genomic DNA"
                     /organism="Paenibacillus sp. F4"
                     /strain="F4"
                     /submitter_seqid="F4_contig_30"
     gene            <1..>2085
                     /locus_tag="C1T21_RS26040"
                     /old_locus_tag="C1T21_26045"
     region          1..2085
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..2085
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..2085
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[2:>2085](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3..>2085
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS             3..>2085
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: PCP (80-148). E-value: 9.1e-25. Score:
                     78.7. Matches aSDomain: nrpspksdomains_C1T21_RS26040_PCP.1"
                     /NRPS_PKS="Domain: Heterocyclization (168-466). E-value:
                     1.3e-95. Score: 312.1. Matches aSDomain:
                     nrpspksdomains_C1T21_RS26040_Heterocyclization.1"
                     /codon_start=2
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.5"
                     /locus_tag="C1T21_RS26040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C1T21_26045"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_146032523.1"
                     /sec_met_domain="PP-binding (E-value: 3.2e-20, bitscore:
                     58.8, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 2.6e-53, bitscore:
                     167.1, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 2.4e-14, bitscore:
                     38.7, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHRNHVFLCAYYVPDEEEVTPEELRIYLQRELPSYMVPSHYMELE
                     KLPLSPNGKVNRRELPIPDVEQQMDTKYEEARNETEQKLTEIWKEVLGVERIGIHDHFF
                     ELGGNSILMVQIRTRISREMGIDLNLRDFLQHYTIAKLSERIREGVDTQEAIVYPELTP
                     DPEHMYEPFPLTDVQMAYLMGREDHFEMGGVSTHGYTEIKTDFDIDRLNFALQQVIQYQ
                     PMMRAVVFPTGEQVILENVPEYRITVVDLTHMSSSEQQERIVSERERMSHYVFPTDQWP
                     LFEYKAFKLSEGIHYLFISYDMLITDGASIQLIGKLLVKFYHEPNWMPSQLQSTFRDYI
                     LALQHFKNSETYKKDKLYWLNKLEDFPAAPELPLKTDPSGVTEPRFRRLEQEIDRETWE
                     RIKKQAYKYNITPTAALLAAYAEVVAFWSNQPHLAVSLTVFNRLPFHQDVNDMVGDFTS
                     VILIEIDFRSEKGYWERAKQIQERLLESLEHRHYDGIELIREISKYHNYGNRAVMPIVF
                     TSMLLGTKERGEVSWYELGEIQMGAGQTSQVYLDHQLFEDNGSLRLVWDYVEQLFDENV
                     IRTMFKQYIDALTLHTNCIKITEQEMKLLESYNNTEKPIPYSTLHRLFEEQAGRSPEAV
                     ALMYKNESLTYGELQKRSNQIARYLQEQGVCRGDYVSVLASRSIATVANVIGILKAGAA
                     "
     aSDomain        244..447
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_C1T21_RS26040_PCP.1"
                     /evalue="9.10E-25"
                     /label="C1T21_RS26040_PCP.1"
                     /locus_tag="C1T21_RS26040"
                     /protein_end="148"
                     /protein_start="80"
                     /score="78.7"
                     /tool="antismash"
                     /translation="EQKLTEIWKEVLGVERIGIHDHFFELGGNSILMVQIRTRISREMG
                     IDLNLRDFLQHYTIAKLSERIRE"
     aSDomain        508..1401
                     /aSDomain="Heterocyclization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_C1T21_RS26040_Heterocyclization.
                     1"
                     /evalue="1.30E-95"
                     /label="C1T21_RS26040_Heterocyclization.1"
                     /locus_tag="C1T21_RS26040"
                     /protein_end="466"
                     /protein_start="168"
                     /score="312.1"
                     /specificity="C activity: active"
                     /specificity="Active site motif: DMLITDG"
                     /tool="antismash"
                     /translation="YEPFPLTDVQMAYLMGREDHFEMGGVSTHGYTEIKTDFDIDRLNF
                     ALQQVIQYQPMMRAVVFPTGEQVILENVPEYRITVVDLTHMSSSEQQERIVSERERMSH
                     YVFPTDQWPLFEYKAFKLSEGIHYLFISYDMLITDGASIQLIGKLLVKFYHEPNWMPSQ
                     LQSTFRDYILALQHFKNSETYKKDKLYWLNKLEDFPAAPELPLKTDPSGVTEPRFRRLE
                     QEIDRETWERIKKQAYKYNITPTAALLAAYAEVVAFWSNQPHLAVSLTVFNRLPFHQDV
                     NDMVGDFTSVILIEIDF"
     CDS_motif       595..663
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C1T21_RS26040_0001"
                     /evalue="2.30E-03"
                     /label="C2_starter_024-063"
                     /locus_tag="C1T21_RS26040"
                     /protein_end="220"
                     /protein_start="197"
                     /score="10.7"
                     /tool="antismash"
                     /translation="GYTEIKTDFDIDRLNFALQQVIQ"
     CDS_motif       670..708
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C1T21_RS26040_0002"
                     /evalue="1.10E-03"
                     /label="Cy2"
                     /locus_tag="C1T21_RS26040"
                     /protein_end="235"
                     /protein_start="222"
                     /score="11.7"
                     /tool="antismash"
                     /translation="PMMRAVVFPTGEQ"
     CDS_motif       889..933
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C1T21_RS26040_0003"
                     /evalue="1.20E-04"
                     /label="Cy3"
                     /locus_tag="C1T21_RS26040"
                     /protein_end="310"
                     /protein_start="295"
                     /score="15.4"
                     /tool="antismash"
                     /translation="YLFISYDMLITDGAS"
     CDS_motif       1228..1287
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C1T21_RS26040_0004"
                     /evalue="8.00E-08"
                     /label="Cy4"
                     /locus_tag="C1T21_RS26040"
                     /protein_end="428"
                     /protein_start="408"
                     /score="25.0"
                     /tool="antismash"
                     /translation="NITPTAALLAAYAEVVAFWS"
     CDS_motif       1339..1398
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C1T21_RS26040_0005"
                     /evalue="9.60E-09"
                     /label="Cy5"
                     /locus_tag="C1T21_RS26040"
                     /protein_end="465"
                     /protein_start="445"
                     /score="27.4"
                     /tool="antismash"
                     /translation="HQDVNDMVGDFTSVILIEID"
     CDS_motif       1462..1503
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C1T21_RS26040_0006"
                     /evalue="4.40E-05"
                     /label="afterCy5"
                     /locus_tag="C1T21_RS26040"
                     /protein_end="500"
                     /protein_start="486"
                     /score="16.2"
                     /tool="antismash"
                     /translation="LEHRHYDGIELIRE"
     CDS_motif       1540..1566
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C1T21_RS26040_0007"
                     /evalue="5.90E-03"
                     /label="Cy6"
                     /locus_tag="C1T21_RS26040"
                     /protein_end="521"
                     /protein_start="512"
                     /score="9.5"
                     /tool="antismash"
                     /translation="MPIVFTSML"
     CDS_motif       1633..1677
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C1T21_RS26040_0008"
                     /evalue="1.20E-04"
                     /label="Cy7"
                     /locus_tag="C1T21_RS26040"
                     /protein_end="558"
                     /protein_start="543"
                     /score="15.1"
                     /tool="antismash"
                     /translation="QTSQVYLDHQLFEDN"
ORIGIN
        1 ggatcatcgc aatcacgtat ttctatgtgc gtactatgtt ccagatgagg aggaagtgac
       61 cccggaagaa ctgcgaatat atttacaacg ggaactgccg agttatatgg tgccgtcgca
      121 ttatatggaa ctggagaaac taccattatc tcctaatggg aaggtgaatc gaagagagct
      181 gccgatcccg gatgtggagc agcaaatgga tacgaaatac gaagaagcac gtaatgaaac
      241 agagcagaaa ctgacggaaa tatggaaaga ggtattggga gtcgaacgaa ttggaatcca
      301 tgatcatttc tttgaactgg gtgggaattc gattttgatg gtgcaaatcc gaaccagaat
      361 aagtagggaa atgggaatcg atttgaatct acgagacttc ctgcagcatt atactatagc
      421 gaagctgtcg gaacggatca gggaaggtgt agatacacaa gaggccattg tgtatccaga
      481 gttaacacct gatccggagc atatgtatga accatttccg ctaacggatg tgcagatggc
      541 ttatctgatg ggacgcgaag accattttga aatgggcgga gtatcgactc atggatatac
      601 ggaaattaaa actgactttg atatagaccg gcttaatttt gcattacaac aggttattca
      661 atatcagccc atgatgcgcg cagtcgtatt tcctaccggg gagcaggtta ttctggagaa
      721 cgtaccggaa tatcgaataa cagtggttga ccttacccat atgagttcat ctgagcagca
      781 agaacggata gtgtcagaaa gagagcgaat gtctcattat gtatttccaa cagaccagtg
      841 gccgctcttt gaatataaag cttttaaatt atctgaagga attcattatt tgtttatcag
      901 ctacgacatg ttgattacgg atggagcaag tattcaactg ataggcaagc tgctggttaa
      961 attctatcat gagcccaatt ggatgccatc acagttgcag agcacatttc gggactacat
     1021 tctggcattg cagcatttta aaaattcgga aacctataag aaggacaaac tgtattggtt
     1081 gaataaattg gaggatttcc ctgcagctcc agagcttcct ttgaaaacag atccttccgg
     1141 tgtgactgag cctcgttttc gtcgtctgga gcaagagatt gatagagaaa cctgggagag
     1201 aattaaaaag caggcgtata agtacaatat aacgcctaca gcggctcttc ttgccgctta
     1261 tgcagaagtg gtggcctttt ggagtaatca gcctcattta gctgtaagtt tgactgtttt
     1321 caacagactg ccgtttcatc aagatgtgaa cgacatggtt ggagatttta cttctgtaat
     1381 ccttatagaa attgatttta gatctgaaaa gggatattgg gagagagcaa aacagatcca
     1441 agaacgtctt ttagaatcac ttgaacatcg gcattatgat ggaattgagt tgatacgaga
     1501 aatatccaaa tatcataatt atggtaatcg tgctgttatg cctattgttt ttacaagcat
     1561 gctgctgggg accaaagaaa gaggtgaagt tagttggtat gaattgggag aaatacaaat
     1621 gggtgctggg cagacatctc aagtgtattt ggaccaccaa ctttttgaag ataacggttc
     1681 gttaagactc gtttgggact acgttgagca actatttgat gaaaatgtaa ttcgtacaat
     1741 gttcaagcaa tatatagatg cattaactct gcatactaat tgcatcaaaa ttactgaaca
     1801 agaaatgaaa ttgctggaaa gctacaataa tacagagaaa ccgataccat acagcacctt
     1861 gcacaggttg ttcgaggagc aggcagggcg tagtccagaa gcagtggcgc taatgtataa
     1921 gaatgaaagc ctcacgtatg gagaactgca gaaacgttcg aatcaaattg cccgctattt
     1981 gcaagagcag ggggtatgcc gtggtgatta tgtcagcgta ttggcaagcc ggagcattgc
     2041 aactgttgct aatgtcatag gtattttgaa ggctggagca gctta
//
