LOCUS       NZ_LJIW01000002        11503 bp    DNA     linear   CON 11-JUN-2024
DEFINITION  Streptomyces malaysiensis strain F913 SMF9132, whole genome shotgun
            sequence.
ACCESSION   NZ_LJIW01000002
VERSION     NZ_LJIW01000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces malaysiensis
  ORGANISM  Streptomyces malaysiensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces violaceusniger group.
REFERENCE   1  (bases 1 to 5400668)
  AUTHORS   Xu,Y., Wei,J., Xie,J., Li,T. and Zhou,Z.
  TITLE     Genome sequence, genome mining and natural product profiling of a
            biocontrol bacterium Streptomyces malaysiensis F913
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5400668)
  AUTHORS   Xu,Y., Wei,J., Xie,J., Li,T. and Zhou,Z.
  TITLE     Genome sequence, genome mining and natural product profiling of a
            biocontrol bacterium Streptomyces malaysiensis F913
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 5400668)
  AUTHORS   Li,T. and Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2015) State Key Laboratory of Silkworm Genome
            Biology, Southwest University, Tian Sheng Road, Beibei, Chongqing,
            Chongqing 400715, China
REFERENCE   4  (bases 1 to 5400668)
  AUTHORS   Li,T. and Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2015) State Key Laboratory of Silkworm Genome
            Biology, Southwest University, Tian Sheng Road, Beibei, Chongqing,
            Chongqing 400715, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-FEB-2015
            Assembly Method                   :: SOAPdenovo v. 2.04
            Expected Final Version            :: yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002891865.1-RS_2024_06_11
            Annotation Date                   :: 06/11/2024 05:20:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,419
            CDSs (total)                      :: 9,336
            Genes (coding)                    :: 9,091
            CDSs (with protein)               :: 9,091
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 6, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 59 of 245
            Pseudo Genes (incomplete)         :: 218 of 245
            Pseudo Genes (internal stop)      :: 14 of 245
            Pseudo Genes (multiple problems)  :: 43 of 245
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:22:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3410712
            Orig. end                         :: 3422215
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LJIW01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11503
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="20"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11503
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11503
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6503](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6503
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    334..347
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: medium, score: 20.04"
                     /tool="antismash"
     misc_feature    1108..1123
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 21.93"
                     /tool="antismash"
     gene            1265..2524
                     /locus_tag="SMF913_RS39080"
                     /old_locus_tag="SMF913_27699"
     CDS             1265..2524
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055544.1"
                     /locus_tag="SMF913_RS39080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMF913_27699"
                     /product="hypothetical protein"
                     /protein_id="WP_231981250.1"
                     /transl_table=11
                     /translation="MAAPIGALTAVAVVVGLLIALTSPGDAGGGTSDGNLPAGAASPKS
                     DRVPREPKKEDKYADWDGEVKVLGDGSTSYTGPQPHQPKPEKLKPGEKPPQFVVFSWDG
                     ALENDDHLFSRFRQVAKESDATMTFFLSGMYLLPKTKSDLYQPPQHKRGSAAISFPTDE
                     HIRETLEQLRGAWLEGNEIGSHFNGHFCGAKGGQDWSTQEWKSETEQAYSFVKRWKTNT
                     GFTDIEPLPFDYDVELVGGRAPCLEGQKTLIPAIKSLGWRYDASSPGDFQLWPSKIDGI
                     WNFPLQLVPYPESEKQVLSMDFNFLFNQSGDSTEGDPAKYAEWERLTRQGYLNGFERAY
                     NGSRAPLFIGNHFETWNGGIYMQAIEDVMRSVCRRDGVRCVSFKQLADWLDAQDPKVLE
                     KLRMLDPAESPDWSTYTKTR"
     gene            complement(2555..4534)
                     /locus_tag="SMF913_RS39085"
                     /old_locus_tag="SMF913_27700"
     CDS             complement(2555..4534)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055543.1"
                     /locus_tag="SMF913_RS39085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMF913_27700"
                     /product="transglycosylase domain-containing protein"
                     /protein_id="WP_102937053.1"
                     /transl_table=11
                     /translation="MRLRRWAAARIRLDYPRTGLRGVRRWLPSWRQIALFFLFCVGSLT
                     GLLSLLYLQTDIPKNLNDFATQQDNVYYWADGTEMARTGPVNRQEVPLKKVPEQVQWAI
                     LAAENASFYSDSGVSPSGLMRAVTRMVTGGETQGGSTITQQYVKNAYLNQRQTFSRKLT
                     EMFMAIKLDDRMSKRDILERYLNTSWFGRGSYGIQRASYAYYGKDVSELNASEGALLAS
                     LLKGAGTFDPTLGAKNHKRAVDRWKWVLDRMVDIGRLSKSERARYTKFPEPKAPPKPAG
                     LTGQVGYLVETAKAYVSSHTEISDADFDLGGYQIHTTFEKPKVQALAKAVEQTRDKLDP
                     ERRPEDRHVRVGAASVAPGGAIRALYGGPGYLEQGFNDANASNVPAGTSFTPFVYAAAL
                     RDGVLLQRNGARTPVTPSTLYDGNNKIAVHTPEGPYWDRGGKIVRATNDGDASYGQVSL
                     RQAIVQSINTPMIQLGMDVGLDRVRRVSIDAGLLPSSFGARVPAFSLGTATPSSIRMAS
                     AYGTFAAEGTHHAPYSVSGLTHGGQKVELRKDRARRAFSAEVAAQVDDALRGAVQSPDG
                     SARGAAMADAEVAGKGGTAQDGTSAWFVGYTKELSTAVSLSRLDPKSQELMPLEGMGGG
                     GADTSGDTYPLDIWKSYMAGRTAE"
     misc_feature    complement(4722..4736)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.81"
                     /tool="antismash"
     misc_feature    complement(4723..4736)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.6"
                     /tool="antismash"
     misc_feature    complement(4724..4744)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 20.73"
                     /tool="antismash"
     gene            5001..6503
                     /locus_tag="SMF913_RS39090"
                     /old_locus_tag="SMF913_27702"
     CDS             5001..6503
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014175675.1"
                     /locus_tag="SMF913_RS39090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMF913_27702"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_102937054.1"
                     /sec_met_domain="DUF692 (E-value: 4.8e-94, bitscore: 312.5,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVRLGTGIGWRPEIADDIARLPGIDWVEVVAENICPDHVPDALRL
                     LRERGTTVVPHGVSLGLGGAERPEPGRLRALAERAEALGSPLVTEHIAFVRAGGTLTAS
                     PSIEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLAVENIAALISWPGEEMTEGQFLA
                     ELVERTGVRLLIDVANLHTNHVNRGEDPAKALDELPTSAIAYVHVAGGVEKDGVWHDSH
                     AHPVTQPVLDVLAELCARTAPPGVLLERDEDFPAAGELAGELDAIRAVTAAGAAEVGTA
                     TGSAKPVTAAGAAEAATAAGAAEAATAAGAAEAATAARTGDEAAAPVWASAAADEAHTG
                     PDAPAADAPAPEAPLPEAPVPDAVRERLALAQTALLSALVGDTPPPEGFDRRRLRVQSA
                     ALTAKRASVIAKVAPELPEILGTGYRPAFVRYAEGRPMTGGYRRDALSFAEHLLADGRP
                     DGSAARRRLTLWWQERSGPAPLPSHPAARFARRVRLALSGGR"
     gene            6509..7519
                     /locus_tag="SMF913_RS39095"
                     /old_locus_tag="SMF913_27703"
     CDS             6509..7519
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009714418.1"
                     /locus_tag="SMF913_RS39095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMF913_27703"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_079153193.1"
                     /transl_table=11
                     /translation="MGTIAGLVYAVVTVSSAMLITGTVRARRRRVSGPVYAQAYDLLEA
                     AFLAGGPGRVADTVISTMYADGRLAIGGPGVVSVRQPVARGPVEEELLRLSANSPHGAL
                     TWLRRELMRSAAVQEIGDRLAGRGLMVRPDALRFWRRMARAHITLCAVGAFLAIMISIG
                     PVTYDGPPLIFMIAPALFIGFLIGRICAGAGKRRLTAAGKRALVAYRRESGVSGRHARA
                     RSIGRPATVSVAVVVALTGAATLTDDPQLRAQLLEAQKVPSGSGSPGYSGSSGDSGFSA
                     SDGGGSWCGGDGGGGGSSCGGSSCGGSSGGGCGGGSGCGGGGGCGGGGGGGCGGG"
     gene            8570..9376
                     /locus_tag="SMF913_RS39100"
                     /old_locus_tag="SMF913_27704"
     CDS             8570..9376
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055540.1"
                     /locus_tag="SMF913_RS39100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMF913_27704"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_099015232.1"
                     /transl_table=11
                     /translation="MLWVLFLLVAWGAAIVSCTRLCLAAVAAAQPMEPDPAADGKGLSL
                     YEAAFLSGGPRRVGDLALVTMCRERRLLLAHTGWVTVVDPEGRDELERSVITAIGPEGQ
                     ALVRPVRAAFATAAPVRALGDRLVAAGLAVPASARTNVAVAVRQVRAACALVLAMAVAA
                     LLLVPPAAGRGPVASWFMLPLLLTVSCLLIARVEAHPYSRWASPAGQQLLGRIDVPPRR
                     TVAQRSGGGPDGGADDADVLTALAVHGTVALSDRALRAALKTHWGH"
     gene            9462..11303
                     /locus_tag="SMF913_RS39105"
                     /old_locus_tag="SMF913_27705"
     CDS             9462..11303
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (170-557). E-value:
                     1.5e-20. Score: 65.8. Matches aSDomain:
                     nrpspksdomains_SMF913_RS39105_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055539.1"
                     /locus_tag="SMF913_RS39105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMF913_27705"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_250850916.1"
                     /sec_met_domain="PF00561 (E-value: 9.3e-24, bitscore: 82.4,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAAALYGILGPLSLSLTMSALLATPATGRPLTAPTAPAATAATP
                     STRAATAPAGAAGAPLTAAPAARSAAPAGPVAAAAPVWTTWSGSSAAAAAPPADPASAR
                     GVALAARRAAERGVTFRRCPRAESLAAPITCGKVSVPLDYARPRGKRVSLTVSRVRASG
                     PKARRQGALIYNPGGPGASGMAFPEYAAEKSFRRLAAVYDFVGYAPRGVGRSAPLSCQR
                     PSAYAKAPTSSPPHPTAAFKRRLVARARAYVRGCVRRSGRALAHYTTLNNARDLEVLRA
                     ALGERRLTFVGASYGTYYGAVYATLFPGRVRRMVFDSVVNPAPWQIWYRDNLDQSLAFE
                     RRWRDWRRWVARHHRVYHLGATADRVLASYEKARRRLARKPAGGVVGTAQLQSAFLRTG
                     YNDAYWDPAARALAAYLLGDSKPLIALAAPDRAQAADDENGNAVYTAVECNDAPWPREW
                     ATWDRDNTALARRAPFETWDNVAMNLPCAFWPVKPGRPLDVGNLEAVGPRTGRVALPPV
                     LLLSAERDAATPYAGAKELWHRLPGSSLVTERKAGTHGLWGGPNGCVNRHVDTYLLTGS
                     TPGRSAYCAPRPAPEPQPEPAPLPDSGKQPAALPGTP"
     aSDomain        9972..11132
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SMF913_RS39105_Abhydrolase_1.1"
                     /evalue="1.50E-20"
                     /label="SMF913_RS39105_Abhydrolase_1.1"
                     /locus_tag="SMF913_RS39105"
                     /protein_end="557"
                     /protein_start="170"
                     /score="65.8"
                     /tool="antismash"
                     /translation="ALIYNPGGPGASGMAFPEYAAEKSFRRLAAVYDFVGYAPRGVGRS
                     APLSCQRPSAYAKAPTSSPPHPTAAFKRRLVARARAYVRGCVRRSGRALAHYTTLNNAR
                     DLEVLRAALGERRLTFVGASYGTYYGAVYATLFPGRVRRMVFDSVVNPAPWQIWYRDNL
                     DQSLAFERRWRDWRRWVARHHRVYHLGATADRVLASYEKARRRLARKPAGGVVGTAQLQ
                     SAFLRTGYNDAYWDPAARALAAYLLGDSKPLIALAAPDRAQAADDENGNAVYTAVECND
                     APWPREWATWDRDNTALARRAPFETWDNVAMNLPCAFWPVKPGRPLDVGNLEAVGPRTG
                     RVALPPVLLLSAERDAATPYAGAKELWHRLPGSSLVTERKAGTHGLW"
ORIGIN
        1 gggctgctgg gagccgtctg ggcggcggcc atggcctgga tctggtcgct ggcgctggtc
       61 gacggctggc agcggggggt ggcggaacgg ctgaccgccc gccatgaata tctgcgcggc
      121 gtcgaccggt tccacgacat cggcgcggcc ctgcgcggct tcaccggcca tatcctgctc
      181 actcagcccg accactggcc cgcccatatc gcgggccatc cgccgggcgc cgtgctcacc
      241 ttcgtgggcc tggaccggct gggcctgggc ggcggcggtt gggccggggc gttctgcgtc
      301 accgtgggcg gctcggcggc ggccgcggtg ctggtcacgc tgcgcgccct ggggcgggag
      361 cgggccgcgc gcgcggcggc cccgtttctg gtcctggcgc cgggggcggt gtgggtgggc
      421 gcctcggccg acggctactt cacggccgtc gcggcgtggg ggctcgcgct gctggcgctc
      481 gcggcgacgc gtacggtccg ggcgcccggg gcggtcgcgt tcggcgccgg actgctgctg
      541 gggctgacgg tgtatctctc ctacgggctg gcgctgctgg ccctgcccgc cgtcgtcgta
      601 ctgctgctcg cccgcaccgc gcggccgctg cccttcgcgc tgaccggggt ggcggtggtg
      661 gccggtgcgt tcaccctggc cggcttccag tggtgggagg ggtactccct gctggtcgag
      721 cgctactacc agggcgtggc cgccgaccgc ccgtacgggt actgggtctg ggccaatccg
      781 gccgtcgcga cggtggccgc cggtccggcc tgtgtcgcgg gtgtgcggcg catgctgtcg
      841 gcggtgccgg atgccgtccg gcggctgcgg ccgggcggac cgggcgcccc gggcggcgtc
      901 ccggacgggc tcgtcgtgct ggccttcgcc gcgctgctcg cgctcgtcgt ggccgatctg
      961 tcagggatga gcaaggccga gacggagcgg atctggcttc ccttcatcct ctggctgctg
     1021 cccaccaccg cgctgctgcc cgccgttgac cgccgccgat ggcttctggc gcaggcgtgt
     1081 ctcgcactgc tcgtcaacca cctgttgttg accggttggt gaggagactg atgtgaagag
     1141 cacgtgatac ttctgtgata ctgagggaga cgtacatctg tactgttgcc agggggcggg
     1201 acccgtgaag gcgctgaaga tcgtgaaagc gttgcaggct ttaagcgcga ggaacgccta
     1261 tggcgtggcc gcgcccatag gcgccctgac ggccgtggcc gtcgtggtgg ggctgctgat
     1321 agccctgacc tcgccggggg acgcgggcgg cggcacgtcg gacgggaacc tccccgccgg
     1381 tgccgcgtcc cccaagtccg accgggtccc cagagagccc aagaaggaag acaagtacgc
     1441 cgattgggac ggcgaggtga aggtgctcgg ggacggctcc acgtcctaca ccggaccgca
     1501 gccgcaccag cccaagcccg agaagctcaa acccggtgag aaaccgccgc agttcgtggt
     1561 cttctcctgg gacggcgcgc tggagaacga cgaccacctc ttctcccgtt tccgccaggt
     1621 cgccaaggag agcgacgcca cgatgacgtt cttcctcagc ggtatgtatc tgctgcccaa
     1681 gacgaagagc gacctctatc agccgcccca gcacaagcgg ggctcggccg ccatctcctt
     1741 ccccaccgat gagcacatca gggagaccct ggagcagctg cgcggtgcct ggctggaggg
     1801 caacgagatc ggctcccact tcaacggaca cttctgcggc gccaagggcg ggcaggactg
     1861 gtccacccag gagtggaaga gcgaaacgga gcaggcgtac tcctttgtga agcggtggaa
     1921 gaccaacacc ggcttcaccg acatcgaacc gctgccgttc gactacgacg tggagctggt
     1981 gggcgggcgc gcaccgtgcc tggagggcca gaagacgctg atcccggcga tcaagtccct
     2041 cggctggcgc tatgacgcga gctcccccgg cgacttccag ctctggccgt ccaagatcga
     2101 cggcatctgg aacttcccgc tccagctggt gccgtacccc gagagcgaga agcaggtcct
     2161 ctcgatggac ttcaacttcc tcttcaacca gtccggtgac agcaccgagg gcgatccggc
     2221 gaagtacgcc gagtgggagc ggctgacccg ccagggctat ctgaacggct tcgagcgcgc
     2281 ctacaacggc agccgggcgc cgctgttcat cggcaatcac ttcgagacct ggaacggcgg
     2341 catctatatg caggccatcg aggacgtgat gaggagcgtc tgccgccgcg acggagtgcg
     2401 atgcgtgtcg ttcaagcagc tcgccgactg gctggacgcc caggacccga aggtcctgga
     2461 gaagctgcgg atgctggacc cggcggagtc gcccgactgg tcgacgtaca ccaagaccag
     2521 gtaagcggtc ccgttgccgg gcttccgccg tcgctcattc cgccgtacgg cccgccatat
     2581 agctcttcca gatgtccagg gggtaggtgt cacccgaagt gtcggcgccg cctccgccca
     2641 tcccctcgag cggcatcagc tcctgcgact tggggtccag ccgggacagc gagaccgccg
     2701 tcgagagctc cttggtgtag ccgacgaacc aggcggaggt gccgtcctgc gccgtgccgc
     2761 ccttaccggc cacctcggca tcggccatgg ccgccccgcg cgccgagccg tccgggctct
     2821 ggaccgcgcc gcgcagcgcg tcgtccacct gtgccgcgac ctccgcgctg aaggcccgtc
     2881 tggcgcggtc cttacggagc tcgaccttct gcccgccgtg ggtgagcccg ctcaccgagt
     2941 acggcgcgtg gtgcgtgccc tcggcggcga acgtcccgta ggcgctggcc atacggatcg
     3001 agctgggcgt cgcggtgccg agcgagaacg cgggcacccg cgcgccgaag ctgctgggca
     3061 gcagccccgc gtcgatggac acccgccgga cccggtccag gccgacgtcc atgcccagct
     3121 ggatcatcgg ggtgttgatg gactgcacga tcgcctggcg cagcgagacc tgcccatagg
     3181 aggcgtcgcc gtcattggtg gcccgcacga tcttgccgcc gcggtcccag tacggcccct
     3241 cgggcgtatg caccgcgatc ttgttgttgc cgtcgtagag cgtcgagggc gtcacgggcg
     3301 tccgtgcccc gttgcgctgg agcagtacgc cgtcgcgcag cgcggcggcg tagacgaagg
     3361 gggtgaagga ggtgccggcc gggacgttgg aggcgttcgc gtcgttgaat ccctgctcga
     3421 gatagccggg gccgccgtac agcgcgcgta tcgcgccgcc cggggccacc gaggcggcgc
     3481 ccacccgcac atgccggtcc tcgggacgcc gctcgggatc gagcttgtcc cgggtctgct
     3541 ccaccgcctt ggcgagcgcc tggaccttcg gcttctcgaa ggtcgtgtgg atctgatagc
     3601 cgcccaggtc gaaatcggcg tcggatatct cggtatggga gctcacatac gccttggccg
     3661 tctccaccag atagccgacc tgaccggtga gcccggccgg cttggggggc gccttgggct
     3721 ccgggaactt cgtatagcgc gcccgctcgg acttcgacag ccttccgatg tcgaccatcc
     3781 ggtccagcac ccacttccag cggtcgacgg cccgcttgtg gttcttggcg ccgagcgtcg
     3841 gatcgaatgt cccggcgccc ttgagcagcg aggcgagcaa cgcgccctcg ctcgcgttga
     3901 gttccgagac gtccttgccg tagtacgcgt acgaggcccg ctgaatgccg tagctgccgc
     3961 gcccgaacca gctggtgttc agatagcgct cgaggatgtc ccgcttgctc atccggtcgt
     4021 ccagcttgat ggccatgaac atctcggtga gcttgcggga aaaggtctga cgctggttca
     4081 gataggcgtt cttcacatac tgctgggtga tggtggagcc gccctgggtc tcaccgcccg
     4141 tcaccatccg tgtcaccgcg cgcatcagtc cgctcgggga caccccgctg tcggagtaga
     4201 agctggcgtt ctctgcggcg aggatcgccc actggacctg ctccggcacc ttcttcaggg
     4261 gcacctcctg ccggttgacc ggaccggtgc gggccatctc ggtgccgtcc gcccagtagt
     4321 agacgttgtc ctgctgggtg gcgaagtcgt tgaggttctt ggggatgtcg gtctggagat
     4381 acagaaggct cagcagaccg gtcagactgc cgacgcagaa caggaagaac agggcgatct
     4441 gacgccacga cggcagccac cgcctcacac cacggagccc cgtccggggg tagtccagcc
     4501 ggatccgcgc cgcggcccag cggcgcagtc gcatcctccg gctcttcgag gacggcttgc
     4561 ggcggtgctt acggtgcttc cccatggcgt tgtcgctgcc cggcacgtgt ttcccccacg
     4621 ctcgctccgc gcgtgggcgc ggcacaaagg tgtgatcttc catcccccca gaccagaccg
     4681 atcctatttt ccctctgaac atctgtcgcc acagggcccc aaatttttgt aacagtgacc
     4741 atattcagcg acacgagcca cccggtgacc attcgcaccg cctgtgcggt ggccgtggca
     4801 tggccgcctg tgcggtgagc gtgcgcacgg ccgtcggcga ccggccgcgc ggcggcctcc
     4861 gctccctccg tgacaccccg aagctcaaat gaacctctga accgcgaggc ccggggattc
     4921 gtcctccctc tacggggtca tcaccccggc acgcgagaac acaccgcggg ggacgcggta
     4981 cggaacgagg agggggagcc atggtgcgcc tcggtaccgg aatcggctgg cgaccggaga
     5041 tcgccgatga catcgcgcga ctgcccggca tcgactgggt ggaggtggtg gcggagaaca
     5101 tctgccccga ccatgtgccg gacgcgctcc ggctgctgcg cgagcgcggg accacggtgg
     5161 tcccgcatgg ggtgtcgctc ggcctcgggg gagccgagcg gcccgaaccc ggccgtctgc
     5221 gcgcgctggc cgagcgggcc gaggcgctgg gctcgccgct ggtcaccgag cacatcgcgt
     5281 tcgtcagggc cggggggacg ctgaccgcgt caccgtccat cgaggccggg cacctgctgc
     5341 cggtgccgcg cacgcgtgac gcgctggacg tgctgtgcga gaacgtgcgc atcgcgcagg
     5401 acgcgctgcc ggtgccgctc gcggtggaga acatcgcggc gctgatctcc tggcccggcg
     5461 aggagatgac cgagggtcag ttcctggccg agctcgtgga gcgcacgggg gtgcggctgc
     5521 tcatcgacgt cgccaatctg cacaccaacc acgtcaaccg gggcgaggac ccggccaagg
     5581 cgctggacga gctgcccacg tcggcgatcg cctatgtcca tgtggcgggc ggggtggaga
     5641 aggacggcgt gtggcacgac agccacgccc atccggtgac ccagccggta ctcgacgtcc
     5701 tcgccgaact gtgcgcccgt acggcaccgc ccggcgtact cctggaacgc gacgaggact
     5761 tcccggcggc cggggagctg gcgggcgagc tggacgcgat ccgcgcggtg acggcggcgg
     5821 gggcagcgga ggtggggacg gcgacgggga gcgcgaagcc ggtcacggcc gcgggggcgg
     5881 cggaagcggc cacggcggcg ggggcagcgg aagcggccac ggcggcgggg gcagcggaag
     5941 cggccacggc ggcgcggacc ggggatgagg ccgccgcccc ggtgtgggcc tccgccgcgg
     6001 ccgacgaggc ccacaccgga cccgacgccc ccgcggccga cgcccccgcg cccgaggcac
     6061 cgctgcccga ggcaccggtg ccggacgccg tccgtgagcg gctggcgctc gcccagaccg
     6121 cgctgctgtc cgcgctggtc ggggacaccc cgccgcccga gggcttcgac cgtcggcggc
     6181 tgcgggtgca gagcgcggcg ctgacggcca agcgggcgtc cgtgatcgcg aaggtggccc
     6241 cggagctgcc ggagatcctg ggcaccggct accgcccggc gttcgtgcga tacgccgagg
     6301 gccgcccgat gaccggcggc taccgccgcg acgcgctgtc gttcgccgag catctgctgg
     6361 cggacggccg gcccgacggc tcggccgccc ggcgccggct gacgctgtgg tggcaggagc
     6421 ggtccgggcc cgcgcccctg ccgtcccatc cggcggcgcg gttcgcccgc cgggtccggc
     6481 tcgcgctgag cggggggcgg tgaacggcat gggcacgatc gcgggcctgg tctacgcggt
     6541 ggtcaccgtg tcgtcggcga tgctgatcac cggtacggtc cgggcccggc gcaggagggt
     6601 ctcggggccg gtatacgccc aggcgtacga cctgctggag gccgcgttcc tggcgggcgg
     6661 accgggccgg gtggcggaca ccgtgatcag cacgatgtac gcggacgggc ggctggcgat
     6721 cggcggcccc ggcgtggtct cggtgcggca gccggtcgcg cgcggccccg tcgaggagga
     6781 gttgctgcgg ctgtccgcca actccccgca cggcgccctg acctggctgc gccgggaact
     6841 gatgcgcagc gcggcggtgc aggagatcgg cgaccggctg gcggggcgcg ggctgatggt
     6901 acggccggac gccctgaggt tctggcggcg catggcacgg gcgcacatca cgctctgcgc
     6961 ggtgggagcc ttcctggcca tcatgatcag catcggaccc gtcacctacg acgggcctcc
     7021 actgatcttc atgatcgccc ccgcgctctt catcggcttc ctcatcggca gaatctgcgc
     7081 cggtgcgggc aagcgcaggc tgaccgccgc cgggaagcgg gcgctggtgg cgtaccggag
     7141 ggagtcgggg gtcagcggac gccatgcccg cgcccggtcc atcggccgtc cggcgacggt
     7201 ctccgtggcg gtcgtcgtcg cgctgaccgg ggcggccacg ctgacggacg atccgcagct
     7261 gcgggcccag ctgctggagg cgcagaaggt accgagcggc tcgggatcgc ccggctactc
     7321 ggggtcttcg ggcgattcgg ggttctcggc ttcggacggc ggcggatcgt ggtgcggcgg
     7381 ggacggcgga ggcgggggct cctcgtgcgg cggctcgtcc tgcggaggga gcagcggcgg
     7441 aggctgcggc ggaggttcgg gctgcggagg gggcggaggc tgcggcggag gtgggggcgg
     7501 cggctgcgga ggcggctgag ccgccacaca agtggccaca ctgcggccag gaggcatggc
     7561 cgagcccgcc ggggcccggc gggacgccgg gggcaggcgc gacgccggag gcaggcgcga
     7621 tgccggggcc gggcgagatg ccggaggcag gcgggacgcc gggggggggc gcgcgacgcc
     7681 ggacgcgggc gcgatgccgg gggcaggcga gatgccgggg ccgggcgaga tgccggacgc
     7741 gggcgcgacg ccgggggcag gcgcgacgcc ggacgcgggc gaggggccgg acgcgggcgg
     7801 ccgaccgagc agcggcgggt cgagccgccc agccccgggt gccgatggcg accggcaaga
     7861 ggccctcgcc gatcccagac cgcccgccca ccggcatccc gcgccgcagc tgtacggatc
     7921 gtccaagacc ccaccgcggt cccgagcaga gttacggggt gtccggcgga ccacgcggcg
     7981 gagccgcata ccgatgcttt cccctccccg ccccttcccg ataccggggg ctccgccccc
     8041 ggaccccggg gtcaaggacg gagacccgcc acgcggcgga gccgcatacc gatgccgcgg
     8101 gcaggagcgg aggccctgcc ccggagcccg gagcagaacc ccgaccccga gcccgaggcg
     8161 aagcaccgac cccgagcccg gggcggaggc cctgacccga aacccgaggc gaagccccaa
     8221 ccccgagccc aggcagaacc ccgacccgga gcccagacaa tgccccggcc cggggcccgg
     8281 gcaaagcccc gacccgaaac ccggaacaga accccgaccc ggggcctggg gcggagcccc
     8341 ggtttcgggg gctgggggcg gagccccggt ttcgggggct gggggcggag cccctatacg
     8401 gggtctgggg cggagcccca gttttgggaa ggggcggggc aggggaacag tccgccgtag
     8461 gcggcacaat ccgtcgcggg tctgcgcggc gcgcgcgccg cattcgcgcg agtcgttcat
     8521 atcgtgaaaa ccgtggcgcg gtggtggccg ggtcgagtag aactcggcca tgttgtgggt
     8581 cctcttcctg cttgtcgcgt ggggcgcggc gatcgtcagt tgcacgcgtc tgtgcctggc
     8641 cgcggtcgcc gccgctcagc cgatggagcc tgatccggcg gcggacggga agggcctgag
     8701 cctgtacgag gcggcgtttt tgtccggcgg cccgcggcgg gtgggggatc tggcccttgt
     8761 gacgatgtgc cgcgagcgcc ggctgctgct cgcccacacc ggctgggtca ccgtggtgga
     8821 cccggagggg cgggacgagt tggagcggtc cgtgatcacc gcgatagggc cggagggcca
     8881 ggccctcgtg cggccggtgc gggccgcgtt cgccaccgca gccccggtgc gggcgctggg
     8941 cgaccggctg gtggcggcgg ggctcgcggt gcccgcctcg gcccgtacga atgtggcggt
     9001 cgcggtgcgg caggtacggg cggcgtgcgc gctggtcctg gcgatggcgg tcgccgcgct
     9061 gctgctggtg ccgcccgcgg cggggcgcgg tccggtggcc tcgtggttca tgctgccgct
     9121 gctgctgacg gtgagctgtc tgctgatcgc ccgggtcgag gcgcatccgt actcacggtg
     9181 ggcctccccc gccggtcagc aactgctggg ccggatcgac gtgcctccgc ggcggacggt
     9241 cgcccagcgt tccggcggcg gcccggacgg cggtgccgac gacgccgatg tgctcaccgc
     9301 gctcgcggtg cacggcaccg tcgcgctctc cgaccgggcg ctgcgcgcgg cgctgaagac
     9361 gcattggggt cattgacccc gacaccgcgg cccggtgctt tacatggtca tccgccgcgc
     9421 caaatatccc tttcatcgcg cgacgcacga agggatggcc agtgagagca gcagcgctgt
     9481 acggcatcct cggaccgctg tccctgtcgc tgaccatgag cgcgctgctc gcgacgcccg
     9541 ccaccggccg cccgctcacc gcgcccaccg cccccgccgc cacggctgcc accccgtcca
     9601 ccagggccgc cacggccccg gccggtgcgg cgggcgcacc gctcacggcg gcccccgcgg
     9661 cccgttcggc ggcccccgcc ggccccgtcg ccgccgccgc gcccgtctgg accacctggt
     9721 ccgggtcgtc cgccgcggcc gccgccccgc ccgctgaccc ggcctcggcc cgcggggtgg
     9781 cgctcgccgc ccggcgcgcg gcggagcgcg gtgtcacctt ccgccgctgt ccccgcgccg
     9841 agtcgctggc cgcgcccatc acctgcggaa aggtctccgt accgctcgac tacgcccgtc
     9901 cccgcggcaa gcgcgtctcc ctcaccgtca gccgggtccg ggcgagcggc cccaaggcgc
     9961 ggcgtcaggg cgcgctgatc tacaacccgg gcggaccggg cgcgtccggt atggcgttcc
    10021 ccgagtacgc ggccgagaag tccttccgcc gcttggccgc cgtctacgac ttcgtcggct
    10081 acgccccacg cggcgtgggc cgctccgccc cgctctcctg ccagcggccc tcggcctacg
    10141 ccaaggcgcc gaccagttcc ccaccgcacc ccaccgccgc cttcaaacgg cggctggtgg
    10201 cgcgggcgcg cgcctatgtg cggggctgtg tgcgccgctc gggccgcgcg ctcgcgcact
    10261 acacgacgct gaacaacgcc cgcgatctgg aggtcctgcg cgcggcgctg ggcgagcggc
    10321 ggctgacctt cgtgggcgcc tcgtacggca cgtactacgg ggccgtgtac gccacgctct
    10381 tcccggggcg ggtgcgccgg atggtcttcg acagcgtggt caacccggcc ccctggcaga
    10441 tctggtaccg cgacaacctc gaccagtcgc tggccttcga gcggcgctgg cgggactggc
    10501 gccgctgggt cgcccggcac caccgcgtct accacctggg cgccaccgcg gaccgggtgc
    10561 tcgcctcgta cgagaaggcg cggcggcggc tggcgcgcaa gccggcgggc ggggtcgtcg
    10621 gcaccgccca gctccagagc gcgttcctca ggaccggcta caacgacgcc tactgggatc
    10681 cggccgcccg ggcgctggcc gcctatctgc tcggtgactc caagccgctg atcgcgctcg
    10741 ccgcgcccga tcgggcgcag gcggccgacg acgagaacgg caacgccgtc tacaccgccg
    10801 tggagtgcaa cgacgcgccc tggccgcgcg agtgggccac ctgggaccgc gacaacaccg
    10861 ccctcgcccg ccgcgcgccc ttcgagacct gggacaacgt ggcgatgaac ctgccgtgcg
    10921 ccttctggcc ggtgaagccg ggccgccccc tcgacgtggg gaacctggag gccgtgggcc
    10981 cccggacggg gcgggtcgcg ctgccgccgg tgctgctgct gtcagcggag cgggacgcgg
    11041 ccaccccgta cgcgggggcg aaggagctgt ggcaccggct gcccggctcc tcgctggtga
    11101 ccgagcggaa ggcgggcacc cacggtctgt ggggcggtcc gaacggctgc gtcaaccgcc
    11161 atgtggacac ttatctgctg accggtagta cccccggccg gtccgcctac tgcgccccgc
    11221 gcccggcgcc cgaaccacag ccggaaccgg ccccgctgcc cgattcgggc aagcagccgg
    11281 ccgcgctccc gggcacgccg taagagggcg ccgaagcccg tgcgggacgg ccgccggacc
    11341 gtcccgcggc ggccttacgg ccacccctgc ggccgtaagg tcacgcccgg aggccgtaag
    11401 gatcccggcg cccgccgtaa gagcgtcccg gcccgccgta aaggcgtccc cggccgcccc
    11461 aagagcgtcc gtcccgagcg ccgtaagggc gtcccggccg cct
//
