LOCUS       NZ_LJIW01000002        10936 bp    DNA     linear   CON 11-JUN-2024
DEFINITION  Streptomyces malaysiensis strain F913 SMF9132, whole genome shotgun
            sequence.
ACCESSION   NZ_LJIW01000002
VERSION     NZ_LJIW01000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces malaysiensis
  ORGANISM  Streptomyces malaysiensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces violaceusniger group.
REFERENCE   1  (bases 1 to 5400668)
  AUTHORS   Xu,Y., Wei,J., Xie,J., Li,T. and Zhou,Z.
  TITLE     Genome sequence, genome mining and natural product profiling of a
            biocontrol bacterium Streptomyces malaysiensis F913
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5400668)
  AUTHORS   Xu,Y., Wei,J., Xie,J., Li,T. and Zhou,Z.
  TITLE     Genome sequence, genome mining and natural product profiling of a
            biocontrol bacterium Streptomyces malaysiensis F913
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 5400668)
  AUTHORS   Li,T. and Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2015) State Key Laboratory of Silkworm Genome
            Biology, Southwest University, Tian Sheng Road, Beibei, Chongqing,
            Chongqing 400715, China
REFERENCE   4  (bases 1 to 5400668)
  AUTHORS   Li,T. and Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2015) State Key Laboratory of Silkworm Genome
            Biology, Southwest University, Tian Sheng Road, Beibei, Chongqing,
            Chongqing 400715, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-FEB-2015
            Assembly Method                   :: SOAPdenovo v. 2.04
            Expected Final Version            :: yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002891865.1-RS_2024_06_11
            Annotation Date                   :: 06/11/2024 05:20:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,419
            CDSs (total)                      :: 9,336
            Genes (coding)                    :: 9,091
            CDSs (with protein)               :: 9,091
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 6, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 59 of 245
            Pseudo Genes (incomplete)         :: 218 of 245
            Pseudo Genes (internal stop)      :: 14 of 245
            Pseudo Genes (multiple problems)  :: 43 of 245
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:22:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2327641
            Orig. end                         :: 2338577
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LJIW01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10936
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="14"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10936
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10936
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5936](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5936)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            676..2190
                     /locus_tag="SMF913_RS34250"
                     /old_locus_tag="SMF913_26757"
     CDS             676..2190
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056770.1"
                     /locus_tag="SMF913_RS34250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMF913_26757"
                     /product="NADH-quinone oxidoreductase subunit NuoF family
                     protein"
                     /protein_id="WP_069867543.1"
                     /transl_table=11
                     /translation="MITTKPRLACVDPPRLLAGLDEVYRLDRVGHLTVHGTMPALRADE
                     LVALAENIDLRGRGGAGFPFAKKVQAVVESAGRREGRCAVVVNGSEGEPSCLKDTALLL
                     HTPHLVIDGAVLAAEAIGAEEVAIAVHRPDVEESLAAAIAERGPSGVPVRVSRTPDRFV
                     AGEGGAVINGISGAPAIPNGRKIRTSDSGLSGLPTLLSNTETFAQLAVAARMGALPYRS
                     VGLPTEPGTTLLTVGGKYVMETPTGVPLTYVLELCGLTAGQAVLVGGYHGKWLDPRSIN
                     AATISRESMKKYGARLGAGAVLPIPENTCPLGETARVARWLAAETAGQCGPCFIGLPAI
                     ADAIGQVERGGGQAAIDNVRAYVNSVKKRGACSHPDGTAGFVTTALEAFPEEFEAHALG
                     AGCGRPTMGVLPLPEDALPLALPPAPLNPSARDDRSQRGPMEKLLVDWSLCQGHGLCAD
                     ILPPEVLTLGIDGYPASASMEVPRQLRAQAVRAVRRCPALALRIEE"
     gene            2401..3375
                     /locus_tag="SMF913_RS34255"
                     /old_locus_tag="SMF913_26758"
     CDS             2401..3375
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056769.1"
                     /locus_tag="SMF913_RS34255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMF913_26758"
                     /product="SCO0930 family lipoprotein"
                     /protein_id="WP_102936649.1"
                     /transl_table=11
                     /translation="MEKRRHIAFAGVSVAMVLLTAACGSSKDNTAGSANVQPAGGSQTV
                     GAGASASAGAVAGAGGGYEAGGDTGAAAGGAAERTGPAKQLAVKEDAKLGKFITDGKGW
                     TLYRFDEDTPKPAKSNCNDDCATKWPAVPADDAAATTGIDSAKLGSVTRSDGTKQLTLA
                     GWPVYRFAGDDKPGDTKGQGVGGTWNALAPDGKPAGKTAATDDSLQLMVNNNAELGKII
                     VDGDGMTVYRFNKDSAWPMKTGCLGACLDTWKPVKAVDTTKVAGVDAAKVTSFKRPDGT
                     KQAAFDCWLLYTFTGDKKPGDINGQGVKGTWFAVTDKGKKAGQ"
     misc_feature    complement(2414..2424)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 17.67"
                     /tool="antismash"
     gene            3467..3991
                     /locus_tag="SMF913_RS34260"
                     /old_locus_tag="SMF913_26759"
     CDS             3467..3991
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056768.1"
                     /locus_tag="SMF913_RS34260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMF913_26759"
                     /product="hypothetical protein"
                     /protein_id="WP_102936650.1"
                     /transl_table=11
                     /translation="MSLALRATAVCAGLLFLAGCGGNDQAHGARHEQKKISPATKEFAE
                     PGTPAPLTKLADAIGCKATVITEADELRQGACGSGAKRFTMVTFATDKGQHDWLTTSKD
                     YGGMYLVGKRWSVTGLSMNSLEPLREKIGGSLEKGMSHGASHGGKKQEDGMEGMEGMDG
                     SASHGGHDGKK"
     gene            complement(4035..4712)
                     /locus_tag="SMF913_RS34265"
                     /old_locus_tag="SMF913_26760"
     CDS             complement(4035..4712)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056767.1"
                     /locus_tag="SMF913_RS34265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMF913_26760"
                     /product="HAD family phosphatase"
                     /protein_id="WP_069867278.1"
                     /sec_met_domain="HAD_2 (E-value: 4.1e-22, bitscore: 77.1,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAEKFLGWTPAAIVFDCDGTLMDSERHWEEAREVVLRSHGTAPD
                     EEFARRTKGLHYTECGRMLAEFAGRPELAEEMTEQLLDAFRRLVADDPVTMPGAPQLVA
                     KAATFAPLAVASNCPRDVVEDGLAKAGLLRYFGHVLVPEGEVRPKPYPDVYLEAARLCG
                     AAPADALAVEDSHCGFLSASRAGLRVLGVGPRKPVDESPPVDVWVSTLADPDLVKWADS
                     RAA"
     misc_feature    4860..4880
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.82"
                     /tool="antismash"
     gene            complement(5001..5936)
                     /locus_tag="SMF913_RS34270"
                     /old_locus_tag="SMF913_26761"
     CDS             complement(5001..5936)
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056766.1"
                     /locus_tag="SMF913_RS34270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMF913_26761"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_069867279.1"
                     /sec_met_domain="AfsA (E-value: 3.8e-19, bitscore: 66.5,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVIVREAHGLSEPSGPAGRPLSWSRTVAREAVHRVSVAEVLLTDV
                     RTHGDNRFEAAAQWPRSHPTFPHGGDGRHHPLMIAETLRELGIYIPTRYYAVPAGAHFL
                     IRDISYRLDPETEPRAPYGASDITCRAAVTDIRTSPCGRFVTGMRLDVVLSAGGKLFAW
                     AGGTARFLDGTTYAETRAAAHGHTARRRLRAAVRPSPAQLAVPAARDVLVVRDGGVVYL
                     DPADPRHPFFFDHWSDHMPGLVLLEGARQAAALATGGTLIRPVVCRMKAIRFTESYPPA
                     VVECTSHGRTCVFRLRQAGICTAVGVLQYL"
     misc_feature    complement(5935..5948)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 15.6"
                     /tool="antismash"
     misc_feature    5936..5949
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 15.9"
                     /tool="antismash"
     misc_feature    complement(5938..5951)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.73"
                     /tool="antismash"
     misc_feature    complement(5941..5954)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 14.63"
                     /tool="antismash"
     misc_feature    complement(5944..5957)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.28"
                     /tool="antismash"
     misc_feature    5947..5956
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.18"
                     /tool="antismash"
     misc_feature    complement(6018..6034)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 17.49"
                     /tool="antismash"
     gene            6045..6686
                     /locus_tag="SMF913_RS34275"
                     /old_locus_tag="SMF913_26762"
     CDS             6045..6686
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056765.1"
                     /locus_tag="SMF913_RS34275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMF913_26762"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_069867280.1"
                     /transl_table=11
                     /translation="MQERAEQTRRSLLEAAAFLFDERGYAGTSISDITSRSGHTSGAIY
                     FHYTSKERLALAVVEEHFASWPPLIERYTALDSPPLEQLVRLSFAVARAFRDDIVVRAG
                     ARLWAERTLIEAPMPPPFVGWIDAVGQMMEKAGAEGDLAPHVDPLPAARTVVCAFFGLH
                     TVSEALDGRRLVEDRLADLWTLLLPSLQARPGDPAGLLALARADSPPAPE"
     gene            complement(6749..7432)
                     /locus_tag="SMF913_RS34280"
                     /old_locus_tag="SMF913_26763"
     CDS             complement(6749..7432)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (7-163). E-value: 4.3e-11. Score:
                     35.0. Matches aSDomain:
                     nrpspksdomains_SMF913_RS34280_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056764.1"
                     /locus_tag="SMF913_RS34280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMF913_26763"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_102936651.1"
                     /transl_table=11
                     /translation="MAEPRHAVVTGVSSGIGAAIATRLLAEGWRITGLSRTAPAHTVAG
                     SHWIPADLSRPETLPELLAPVSGVDAIVHAAGVQRSARLGELEPEDGALMWRIHVQAAG
                     VLVDTLVDRIADGGRVVLVGSRTMTGVPGKSQYAATKAALPALARSWAAELAPRAVTVN
                     VVAPGPTDTPMLRDPGRSRTPPVLPPLGRLVRPEEVAGLTSFLLGPEGGAVTGQTLVMC
                     AGASL"
     aSDomain        complement(6944..7411)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SMF913_RS34280_PKS_KR.1"
                     /evalue="4.30E-11"
                     /label="SMF913_RS34280_PKS_KR.1"
                     /locus_tag="SMF913_RS34280"
                     /protein_end="163"
                     /protein_start="7"
                     /score="35.0"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VVTGVSSGIGAAIATRLLAEGWRITGLSRTAPAHTVAGSHWIPAD
                     LSRPETLPELLAPVSGVDAIVHAAGVQRSARLGELEPEDGALMWRIHVQAAGVLVDTLV
                     DRIADGGRVVLVGSRTMTGVPGKSQYAATKAALPALARSWAAELAPRAVTVN"
     gene            complement(7425..8630)
                     /locus_tag="SMF913_RS34285"
                     /old_locus_tag="SMF913_26764"
     CDS             complement(7425..8630)
                     /EC_number="3.2.1.18"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009712848.1"
                     /locus_tag="SMF913_RS34285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMF913_26764"
                     /product="exo-alpha-sialidase"
                     /protein_id="WP_102936652.1"
                     /transl_table=11
                     /translation="MTADHDTALPPTDGRLRVRPGDPACQEAFLPAPAVQNHAANLTVL
                     PGGDLGCVWFGGTQEGVPDISVWFSRLAPGSGTWTEPVRLSGDATRSEQNPLLFPTPSG
                     ELWLLYTAQRAGDQDTAEVRLRVSADAGATWDAPRTLFPATEAGGVFIRQPVAVLDSGR
                     WLLPVFHCVATPGVKWAGDHDTSAVMISDDQGRTWRERSVPGSTGCVHMNVHQLPDTTL
                     LALFRSRWADAVYRSHSTDGGETWSEPVRTELPNNNSSVQYVPLADGRLALVYNHSSRA
                     DATARRVSLYDEIDDAGHTARAPAPATARDASAPGAFWGAPRAPMTLALSSDNGLTWPV
                     RRDLDTGDGHCLTNNSRDRLNRELSYPTIRQGPDGTLHIAYTYHRQAIKYVRVAPDWAA
                     DG"
     gene            complement(9209..10228)
                     /locus_tag="SMF913_RS34290"
                     /old_locus_tag="SMF913_26765"
     CDS             complement(9209..10228)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1039: aldo/keto reductase family oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056762.1"
                     /locus_tag="SMF913_RS34290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMF913_26765"
                     /product="aldo/keto reductase"
                     /protein_id="WP_079153198.1"
                     /transl_table=11
                     /translation="MNAHAQTHTTAHASPGGVALPGTPVPLSRLVLGTMTFGDTVDRAG
                     AAAMLDTALDAGVTGVDTANAYAGGTTETILAELLPGRRDRIVLATKAGMPHPDAGDHS
                     PLSARGMRAALEGSLRRLGTDHVDLFYLHQPDRSTPLEETLGTVAEFLAEGKIRALGVS
                     NYAAWQIAEITHTAERVGAPRPVVAQQLYNLLARRIEEEYREYAATSGLRTMVYNPLGG
                     GLLTGRHTFEERPESGRFGDSRVAAMYRERYWDAGLFEAVRDLGRIADEASIPLTDLSL
                     RWLLFRDGVDALLLGGSRIEHLRSNLDAATAGPLPDDVVAACDEVGARLRGPMPAYNR"
     misc_feature    10673..10689
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 19.95"
                     /tool="antismash"
ORIGIN
        1 cttcggcgcg ggtgtgctcg cactggtctg tctcacctcg gcggtgctgt gggggctcgc
       61 cgcgaccgac cagatgatcc tgcacaccag gcagcggctg atggcccagg cgatccaccg
      121 ggcggccgcg gtcgccgggt tgctgttcct ggccgttcac atctgggtca aggtcgcgga
      181 gagccacacc aacgccgcgt ccgccgtact accgttcgcg gacgcaaatc agccggtgct
      241 catcgggctc ggcaccatcg ccggctatct cttcgtgatc gccgcggtga ccggcgccgt
      301 gcgcagctcg ttcgcctcgg cgggcaactc ccgctggcgg taccgctggt ggcgggcgct
      361 gcatgtgggc gcctaccccg cgtggtgcgc cgccctgatc cacggtctga aggcgggccg
      421 cgccgccaag atctgggcca cctacggcta catcgcggcg ctcgccggtg tggccatcgc
      481 gctgtggctg cgtctggtcc agcggcggcg ccaggacatc gaccgggcgg acggcatcga
      541 agtcaggaac ccgcccgccc gcagcatgca ggagggccgg acccggcggg ccgatcccac
      601 cgtgccgcgc cagcgtgcgg ccgagcccgg cccgcgtgac agtcagccgc tcggtgcctc
      661 tcgcgggtgg gaccggtgat cacgaccaaa cccaggctcg cctgtgtcga tccgccccgg
      721 ctgctggccg ggctcgacga ggtgtaccgg ctcgaccgcg tcggccatct gaccgtccat
      781 ggcaccatgc cggccctccg ggcggacgag ctcgtggcgc tcgcggagaa catcgatctg
      841 cgcgggcgcg gtggcgccgg tttccccttc gccaagaagg tccaggccgt ggtggagtcg
      901 gccggccggc gcgagggccg ctgtgccgtg gtggtcaacg gcagcgaggg cgagcccagt
      961 tgtctcaagg acaccgcgct gctgctgcac accccgcatc tggtgatcga cggcgccgtt
     1021 ctggccgccg aggcgatcgg cgccgaggag gtcgccatcg cggtgcaccg cccggatgtc
     1081 gaggagtccc tggccgccgc gatcgccgag cgcggcccca gcggggtccc ggtgcgggtg
     1141 agccgcaccc cggaccggtt cgtcgccggt gagggcggcg cggtcatcaa cggcatcagc
     1201 ggcgccccgg ccatccccaa cggccggaag atccggacca gtgacagcgg gctcagcggg
     1261 ctgcccacgc tgctgtctaa caccgagacc ttcgcacagc tcgcggtggc cgcgcggatg
     1321 ggcgccctgc cgtaccgctc ggtggggctg cccaccgaac cgggcaccac gctgctgacg
     1381 gtcggtggaa agtacgtgat ggagacgccc accggggtgc cgctgaccta cgtccttgag
     1441 ctgtgcggcc tcaccgccgg ccaggcggtg ctggtgggcg gctaccacgg caagtggctg
     1501 gaccccagga gcatcaacgc ggccacgatc tcccgcgagt cgatgaagaa gtacggcgcc
     1561 cggctgggcg ccggggcggt gctgcccatc cccgagaaca cctgcccgct cggcgagacc
     1621 gcgcgggtgg cgcgctggct ggccgccgag acggccggtc agtgcgggcc ctgcttcatc
     1681 gggctgccgg cgatcgcgga cgccatcggc caggtggagc gcggcggcgg ccaggccgcg
     1741 atcgacaatg tgcgcgccta cgtcaactcg gtgaagaagc gcggggcgtg cagccatccc
     1801 gacggcaccg ccggtttcgt gaccaccgcg ctggaggcgt tccccgagga gttcgaggca
     1861 catgcgctcg gcgccgggtg cggacggccc acgatgggtg tgctgccgct gccggaggac
     1921 gcgctgccac tggccctgcc gcccgccccg ctgaacccct cggcgcgcga cgaccgctcc
     1981 cagcgcgggc cgatggagaa gctcctggtg gactggtcgc tgtgccaggg ccacgggctg
     2041 tgcgcggaca tcctgccgcc cgaggtgctg acgctcggga tcgacggcta tccggcctcg
     2101 gcctcgatgg aagtgccccg gcagctgcgg gcgcaggcgg tccgcgcggt ccgccgctgc
     2161 ccggccctgg cgctgcgcat cgaggagtag ccggaggcga atccatctgg caccaccgca
     2221 ccaccgacac cgcggcggcc gtgagaaacg gccgccgact gctgtgcgct cagcgatccg
     2281 gagttttttg cctccggtga caaattcgca ggccgctgaa cgccggtccg catgggctcc
     2341 gtatgcacat gacgtcggcg caatcaccgc cggaccgatc gagattgcag aggaatggtc
     2401 atggagaagc ggcgtcacat cgcattcgcc ggggtgtcgg tagcgatggt tctgctgacg
     2461 gcggcgtgcg gcagcagcaa ggacaacacc gcgggcagcg cgaatgtgca gcccgcgggc
     2521 ggcagccaga ccgtgggtgc gggcgcctcc gcctcggcgg gcgcggtggc cggcgccggc
     2581 ggcgggtacg aggcgggcgg cgacacgggc gcggccgcgg gcggcgcggc cgagcggacc
     2641 ggtccggcga agcagctggc ggtcaaggag gacgccaagc tgggcaagtt catcaccgac
     2701 ggcaagggct ggacgctcta ccggttcgac gaggacaccc ccaagccggc caagtccaac
     2761 tgcaacgacg actgcgccac caagtggccc gcggtccccg cggacgacgc cgcggccacc
     2821 accggtatcg actccgccaa gctgggctcg gtaacccgct ccgacggcac caagcagctg
     2881 acgctggcgg gctggccggt ctaccgcttc gcgggcgacg acaagccggg tgacaccaag
     2941 ggccagggcg tgggcggcac ctggaacgcg ctggcgccgg acggaaagcc ggccggcaag
     3001 accgcggcca cggacgacag cctccagctg atggtcaaca acaacgccga gctcggcaag
     3061 atcatcgtgg atggcgacgg tatgaccgtc taccggttca acaaggacag cgcctggccg
     3121 atgaagaccg gctgcctcgg tgcctgcctg gacacctgga agcccgtcaa ggccgtggac
     3181 accaccaagg tcgccggggt cgacgcggcg aaggtcacct ccttcaagcg ccccgacggc
     3241 accaagcagg ccgcgttcga ctgctggctg ctgtacacct tcaccgggga caagaagccc
     3301 ggcgacatca acggccaggg cgtgaagggc acctggttcg cggtcaccga caagggcaag
     3361 aaggcgggcc agtagggcgg acaccatact gacggtccac cgcggcgggt ggtccgaggc
     3421 catccgggcc cggaccgccc cgcctgacgt tctacgggag gttgatgtgt cactcgcact
     3481 ccgtgccacc gcggtctgcg ccggcttgct gttcctggcc ggctgcggtg gaaacgacca
     3541 ggcacacggc gccagacatg agcagaagaa gatatccccc gccaccaagg agttcgccga
     3601 gcccggcacg cccgcgccac tgacgaagct cgccgacgcc atcggctgca aggccacggt
     3661 catcaccgag gcggacgagc tgcgccaggg cgcctgcggg tccggtgcga agcggttcac
     3721 gatggtcacc ttcgccaccg acaagggcca gcacgactgg ctgacaacgt cgaaggacta
     3781 cggggggatg tacctggtcg gcaagcgctg gtcggtcacc ggcctgtcca tgaactcgct
     3841 ggagcccctg cgcgagaaga tcggcggatc cctcgagaag gggatgtccc acggcgcctc
     3901 ccacggcggg aagaagcagg aggacggaat ggagggcatg gagggcatgg acggctcggc
     3961 gagccatggc ggccacgacg ggaagaagtg acgcgcggcg cccgccgacc aggcggtgtg
     4021 cgcgggtcag cccctcaggc cgcccgggag tccgcccact tgaccaggtc cgggtcggcg
     4081 agggtggaga cccatacgtc gaccggcggg gattcgtcca ccggcttgcg cggcccgacg
     4141 ccgagcaccc tcagcccggc gcgcgaggcg gacagaaacc cgcagtggga gtcctccacg
     4201 gcgagggcgt ccgcgggggc cgcaccgcac agccgggcgg cctccagata gacatcggga
     4261 tacggcttgg gccgcacctc cccctcgggg accagcacat ggccgaagta gcgcagcagc
     4321 ccggccttgg cgagcccgtc ctcgacgacg tcccgcgggc agttgctggc cacggccagc
     4381 ggcgcgaagg tcgcggcctt cgcgaccagt tggggcgccc ccggcatggt gaccggatcg
     4441 tcggccacca gccggcggaa cgcgtccagg agttgttcgg tcatttcttc ggcgagttcg
     4501 gggcgcccgg cgaattcggc cagcatgcgg ccgcattcgg tgtagtggag ccccttcgtg
     4561 cgccgggcga actcctcgtc gggggcggtt ccatggcttc tgagcaccac ttcacgcgcc
     4621 tcctcccagt gtcgttcgga atccatcagg gtgccgtcgc agtcgaagac gattgcggct
     4681 ggggtccatc cgagaaactt ttcggctgtc atctgtctgc cgctccatgg gaggtggagg
     4741 aatcggcgca gggtgtgcct gtacctgcgg gggccgcgca gtggaaatgt cctggcggct
     4801 cagaagggcg ctgggcgccg accgctgttg tcgtacaggc cgcgagaatc gcctgcccga
     4861 aacataacga tcactacgtt agtttgatga cccgcagtaa gcaaggggtc accgagtgtc
     4921 ggcgttttgt tcgtcgttca gtccgttcgt cgccaaagag ttcgtcgcac ggtgcgttct
     4981 tcgcacggtg cgttcgtcgc tcagaggtac tggagcacgc cgaccgccgt acagataccg
     5041 gcctggcgca gccggaagac gcacgttctg ccgtgggagg tgcactcgac cacggccggc
     5101 ggatacgact cggtgaagcg gatcgccttc atccggcaga cgacgggccg gatcagcgtc
     5161 ccgccggtcg ccagcgccgc cgcctggcgg gcgccctcca gcagcaccag ccccggcata
     5221 tggtcgctcc agtggtcgaa gaagaacgga tggcgggggt cggcggggtc gagatagacc
     5281 actcccccgt cccgcaccac caggacgtcc cgggccgcgg gaacggccag ttgcgcaggt
     5341 gagggccgta ccgcggcgcg cagccgacgg cgcgcggtgt gtccgtgggc ggcggcccgg
     5401 gtctccgcgt aggtcgtgcc gtcgaggaag cgcgcggtgc ccccggccca ggcgaagagc
     5461 ttgccgcccg ccgacagcac gacatccagc cgcatcccgg tgacgaaacg cccgcagggg
     5521 ctggtgcgga tgtcggtgac ggcggcccgg caggtgatgt cggaggcgcc gtacggcgcc
     5581 cggggctcgg tctcggggtc cagccggtag gagatgtccc ggatcaggaa atgcgccccc
     5641 gcgggcaccg cgtagtaccg cgtggggatg tagatcccca gttcgcgcag ggtttccgcg
     5701 atcatcagcg gatggtgacg gccgtccccg ccgtgcggga aggtgggatg ggaccgcggc
     5761 cactgggccg cggcctcgaa tcgattgtca ccgtgcgtac gcacatcggt gagcagcacc
     5821 tcggccaccg agacccggtg caccgcctcc cgcgccaccg tgcgggacca gctgagcggc
     5881 cgcccggcgg gaccggacgg ctccgaaagg ccatgagcct cacgaacgat caccataaaa
     5941 tactaataat gtttgacctg tctcgcctca tcaccatcac tggccgaaag tccgctaagt
     6001 tctccccgtc acatccgatg tgctggtgta aggacggcga acacgtgcaa gaacgggccg
     6061 aacaaacacg ccgatccctt ctggaggccg ccgcattcct tttcgacgaa cggggatacg
     6121 ccggaacgag catcagcgac atcacgtcca ggtccggcca caccagcggc gcgatctatt
     6181 tccattacac gagcaaggaa aggctcgcgc tggccgtggt ggaggagcac ttcgcctctt
     6241 ggcccccgct gatcgagcgc tacaccgccc tcgactcgcc tccgctggag caactggtgc
     6301 ggctcagctt cgcggtggcc cgcgccttcc gcgatgacat cgtggtccgc gccggagccc
     6361 ggctgtgggc cgagcgcacc ctgatcgagg cgcccatgcc accgccgttc gtggggtgga
     6421 tcgacgccgt gggccagatg atggagaagg cgggcgccga gggcgatctg gccccgcatg
     6481 tcgatccgct gcccgccgcc cggaccgtgg tctgcgcctt cttcgggctg cacaccgtct
     6541 ccgaggcctt ggacgggcgg cggctggtcg aggaccgcct ggccgatctg tggaccctgc
     6601 tgctgccctc gctccaggcc cggcccgggg atccggcggg gctgctggcg ctcgcccgcg
     6661 ccgactcgcc gcccgcgccg gagtgaggcg cctgcgccga cccccgccca cgtcggagtg
     6721 aagcaccccc ggcggctccc cggcccggtc agagcgaggc gcccgcgcac atcaccagtg
     6781 tctggccggt gaccgcgccg ccctccgggc ccagcaggaa cgacgtcagc cccgccacct
     6841 cctccgggcg gaccagccgg cccagcggtg gcagcaccgg cggggtgcgg ctacggccgg
     6901 ggtcgcgcag catgggggtg tccgtcggcc ccggcgccac cacgttcacc gtgaccgcgc
     6961 ggggcgcgag ctcggccgcc caggaccggg ccagggcggg cagcgccgcc ttggtggcgg
     7021 cgtactggct cttgcccggg accccggtca tcgtccggct gcccaccagc accacccggc
     7081 cgccgtccgc gatccggtcc accagcgtgt ccaccaggac gcccgccgcc tgcacatgga
     7141 tccgccacat cagcgcaccg tcctcgggct cgagctcccc cagccgcgcc gagcgctgaa
     7201 cgcccgccgc gtgcacgatc gcgtccaccc cggacaccgg ggcgagcagt tcggggaggg
     7261 tctccggccg ggacagatcg gccgggatcc agtgcgaccc cgcgaccgtg tgcgcggggg
     7321 ccgtacggct cagcccggtg atccgccacc cctcggccag cagccgggtg gcgatggcgg
     7381 cgccgatacc cgagctgaca ccggtgacca cggcgtggcg gggctcagcc atcggccgcc
     7441 caatccggtg cgacgcgaac gtacttgatg gcctggcggt ggtaggtgta cgcgatgtgc
     7501 agtgtgccgt cggggccctg ccggatggtg gggtaggaca gctcgcggtt gagccggtcg
     7561 cgtgagttgt tggtcaggca gtggccgtcg ccggtgtcca gatcgcgccg tacgggccag
     7621 gtcaggccgt tgtcggagga cagcgcgagg gtcatcgggg cgcggggtgc gccccagaag
     7681 gcgccgggcg ccgaggcgtc ccgcgcggtg gccggggcgg gggcccgagc ggtgtgcccc
     7741 gcgtcgtcga tctcgtcgta gagggagacg cggcgggccg tggcgtccgc ccggctgctg
     7801 tggttgtaga ccagcgcgag ccggccgtcg gccagcggga cgtactgcac ggaggagttg
     7861 ttgttgggca gttcggtgcg gaccggctcg ctccaggtct ccccgccgtc ggtgctgtgc
     7921 gagcggtaca ccgcgtccgc ccagcggctg cggaagaggg ccagcagggt ggtgtccggc
     7981 agttggtgga cgttcatgtg cacacagccg gtcgagcccg gcacggaccg ctcacgccag
     8041 gtccgcccct ggtcgtcgct gatcatcacc gcgctggtgt catggtctcc ggcccatttg
     8101 accccggggg tggccacgca gtggaacacc ggcagcagcc accgcccgga gtccagaacg
     8161 gccaccggct gacggatgaa caccccaccc gcctcggtgg ccgggaagag cgtacgcggc
     8221 gcgtcccagg tggcgcccgc gtcggcggac acccgcagcc gcacctccgc cgtgtcctgg
     8281 tcacccgccc gctgcgcggt gtagagcaac cacagctcac cggacggggt ggggaacagc
     8341 aacgggttct gctccgagcg ggtggcgtcg ccggagagcc ggaccggctc cgtccaggtg
     8401 ccggagcccg gggcgagccg ggagaaccac acggagatgt ccggtacgcc ctcctgggtg
     8461 ccgccgaacc acacacagcc gagatcccct ccgggcagca cggtgagatt ggcggcatgg
     8521 ttctgcacgg cgggcgcggg cagaaacgcc tcctggcacg ccgggtcacc ggggcgcacc
     8581 cggagccgtc cgtcggtcgg cggcagagcg gtgtcatggt cggcggtcat ggtcgccggg
     8641 ctcctcaggg ttgcggtgat acgggaatgt gtacggccgg ccccaaggac gcgcccgagg
     8701 ggcggctccg ggtgtccggg ggcgggagcc gcatatcgac cgggccggga agtggccttc
     8761 ccccggcccc gacccgcggc cgagccacgg gccccgatac caaccggacc cccggcccgg
     8821 gaggacgcgc ctgacaggcg tcccgggcac agcgggcaaa gccccgcccc gaggagagac
     8881 gccgcccgac ggagtcctgg gccatcgggg tccctcccac ggcaacaccc cgcggccacg
     8941 ccgcacgaca cggaggcggg gcggggcgcc gccgcccggg aaccgggccc gaggggcgtc
     9001 cggggcccaa gggcacaacc cctggttcgg gaaaaggccc tcccacagca acaccctgcg
     9061 gccacggcgg cgaagcgagg cgccgcggtc gcacccacgc ggggcggggc ggggcgctgc
     9121 cgcccaggaa ccgggcccga ggggcgttcg gggtccaggg gcggagcccc ttggttcggg
     9181 aaggggcggg gtggggaaaa ccacgggcct accggttgta cgcgggcatc ggcccacgca
     9241 accgcgcccc cacctcgtca cacgccgcca ccacatcatc cggaagcggc cccgccgtgg
     9301 ccgcgtccag gttggaccgg aggtgctcga tccgggagcc gcccagcagg agggcgtcca
     9361 ctccgtcgcg gaacagcagc cagcgcagcg agaggtccgt cagcgggatg gacgcctcgt
     9421 ccgcgatccg gcccaggtcg cgtacggcct cgaagagtcc agcgtcccag tagcgctccc
     9481 ggtacatggc ggccacccgg gagtcgccga agcggcccga ctccggccgc tcctcaaacg
     9541 tatgacgccc cgtcagcagc ccgccgccga gcgggttgta gaccatggtg cgcagtccac
     9601 tggtggccgc gtactcccgg tattcctcct cgatccgccg cgccagcagg ttgtagagct
     9661 gctgggccac caccgggcgc ggggcgccga cccgctcggc ggtgtgggtg atctcggcga
     9721 tctgccaggc ggcgtagttg gagacgccga gcgcgcggat cttgccctcg gcgaggaact
     9781 cggcgaccgt gcccagcgtc tcctccagcg gtgtggagcg gtcgggctgg tggagataga
     9841 acaggtcgac atggtcggtg cccagacggc gcaggctgcc ttcgagggcc gcgcgcattc
     9901 cgcgcgcgga cagtggtgag tggtcaccgg cgtccgggtg cggcataccg gccttggtgg
     9961 cgagcacgat ccggtcgcgg cggccgggca gcagctcggc caggatcgtc tcggtggtgc
    10021 cgcccgcgta ggcgttggcg gtgtccacgc ccgtcacccc ggcgtccagc gcggtgtcca
    10081 gcatcgcggc ggcgcccgcg cggtccaccg tgtcaccgaa ggtcatggtg ccaagcacca
    10141 gccgcgagag cggcaccggg gttccgggga gggcgacccc gccgggcgac gcgtgggcgg
    10201 tggtgtgggt ctgcgcatgc gcgttcactg cgttgctcct ggttcctgtg gttcccgtgg
    10261 gtcttgtggt tcccatagtt cccgttcccg tggttcctgg tcggtcgcga tcgtggacac
    10321 gggagggccg gaacgtaggg ggtacgggtc agcgttgacc tctcggcagt tctgaggcga
    10381 gcgaccggtt cgctcgactc gctcgacccg tgcggcgccc ggatgacggc cgtacgggtc
    10441 aaagtgtcgc gacggtcacc gtgacatgac tgcgcgcttt acgcaatagc tctcgcgcac
    10501 ttcgcgcaaa gtatgtcatc atcgggacca cggagacgat gtcgccagca gcgcgtcgct
    10561 ccgccggtca tggtcattcg aggaggtacc ggtggacatg gcggccagcc ggaggctgtt
    10621 ggccatcgcg gatgacctgt caggcgccgc ggaagtggcg gcggcgcttc attcgcgcac
    10681 aacacgcagt cgggtgctgc tgataggcag gcccggtgcg gtgaacggcg gggtggcgag
    10741 ggcgctacgc ccccgccacc ccggcgaggc gaccgtcctg gacctcgact cccgctatcg
    10801 cccggccacc gaggcggccg aggcggtccg cgaggcgctg cggctgtcct cgccggacga
    10861 gaacgctcca gatgccctgg acaccctggt actgaagaag atcgactcgc tgctgcgggg
    10921 caatgtcgcc gccgag
//
