LOCUS       NZ_LJIW01000002        10996 bp    DNA     linear   CON 11-JUN-2024
DEFINITION  Streptomyces malaysiensis strain F913 SMF9132, whole genome shotgun
            sequence.
ACCESSION   NZ_LJIW01000002
VERSION     NZ_LJIW01000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces malaysiensis
  ORGANISM  Streptomyces malaysiensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces violaceusniger group.
REFERENCE   1  (bases 1 to 5400668)
  AUTHORS   Xu,Y., Wei,J., Xie,J., Li,T. and Zhou,Z.
  TITLE     Genome sequence, genome mining and natural product profiling of a
            biocontrol bacterium Streptomyces malaysiensis F913
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5400668)
  AUTHORS   Xu,Y., Wei,J., Xie,J., Li,T. and Zhou,Z.
  TITLE     Genome sequence, genome mining and natural product profiling of a
            biocontrol bacterium Streptomyces malaysiensis F913
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 5400668)
  AUTHORS   Li,T. and Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2015) State Key Laboratory of Silkworm Genome
            Biology, Southwest University, Tian Sheng Road, Beibei, Chongqing,
            Chongqing 400715, China
REFERENCE   4  (bases 1 to 5400668)
  AUTHORS   Li,T. and Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2015) State Key Laboratory of Silkworm Genome
            Biology, Southwest University, Tian Sheng Road, Beibei, Chongqing,
            Chongqing 400715, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-FEB-2015
            Assembly Method                   :: SOAPdenovo v. 2.04
            Expected Final Version            :: yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002891865.1-RS_2024_06_11
            Annotation Date                   :: 06/11/2024 05:20:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,419
            CDSs (total)                      :: 9,336
            Genes (coding)                    :: 9,091
            CDSs (with protein)               :: 9,091
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 6, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 59 of 245
            Pseudo Genes (incomplete)         :: 218 of 245
            Pseudo Genes (internal stop)      :: 14 of 245
            Pseudo Genes (multiple problems)  :: 43 of 245
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:22:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1280066
            Orig. end                         :: 1291062
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LJIW01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10996
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="7"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10996
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10996
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5996](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5996)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    541..556
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.62"
                     /tool="antismash"
     misc_feature    complement(542..555)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.58"
                     /tool="antismash"
     gene            545..1732
                     /locus_tag="SMF913_RS30795"
                     /old_locus_tag="SMF913_26067"
     CDS             545..1732
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019527527.1"
                     /locus_tag="SMF913_RS30795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMF913_26067"
                     /product="histidine kinase"
                     /protein_id="WP_250850826.1"
                     /transl_table=11
                     /translation="MPVRMESSRVARCLRPVVRWWRSRGLVTRDRELALVLTALVFVPA
                     LSRISSTFGDLPARHGDAFAVLLSLGQTVPLVVRTRRPAVCLAVVGPSFAVHESLGYPP
                     TFGSMGLYLALYGAGSQLSRHRNVVAALASAGYLAFAITLHTLDSPERFLDYLIFFLAL
                     AAFWLLGGYVRARRADEAERRRLAALAATAAERSRIARELHDVVTHHVTAMVVQADAAQ
                     FLTESPGRVTEGLTAISGTGRQALGELRHLLGVLEATGTSAPTRRTPALGTIEELVART
                     RLGGQPVELVEQGERPPMAAGAELAAYRVVQESLTNAVKHAAGHTTEVWVRYHRDGVDI
                     DVTTDGPSRVAHAPDGRGLNGLRERVGLVGGELEAGARPGGGFSVSARIPAGSGS"
     gene            complement(1862..2725)
                     /locus_tag="SMF913_RS30800"
                     /old_locus_tag="SMF913_26068"
     CDS             complement(1862..2725)
                     /EC_number="2.1.1.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003983473.1"
                     /locus_tag="SMF913_RS30800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMF913_26068"
                     /product="SAM-dependent methyltransferase"
                     /protein_id="WP_102936289.1"
                     /transl_table=11
                     /translation="MTDGHERLAGVSSTALFVGITRAAESRRQDRLFEDPLATRFVDAA
                     GAEQQAAWTQGEGRLFTDAMGDYFALRTRYFDDYFMQACAAGCRQAVLLAAGLDTRAFR
                     LHWPAGTRLFELDQPEVLDFKEHVLQGEVPRCEREVIAADLREDWPALLMKHGFRADEP
                     TAWLVEGILVYLTARDADHLLDRIGALSAPGSHLAVEHVTRSMVNTDQAQEAAAASPGG
                     IMNMLSGLWKNEMAQPPAEWLADHGWSAEEELLTDLAKRHERPVPPAFDPALPGTGRVR
                     LLAADR"
     gene            complement(2834..4090)
                     /locus_tag="SMF913_RS30805"
                     /old_locus_tag="SMF913_26069"
     CDS             complement(2834..4090)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608207.1"
                     /locus_tag="SMF913_RS30805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMF913_26069"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_102936290.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 2.4e-18, bitscore:
                     64.5, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRPVIIDKVRIFDGRAMLGPGSVEFADGVITRVTTEPAPVAPASA
                     DVIDGTGMTLLPGLIDAHTHVFGAESNLELALAFGVTTELDMFMGPPEATQALCAAAGE
                     RADLADMRSSGLVASAPGGHPGVTMPLLPTVAGPERADAFVAERQAEGAHFIKIIVDDG
                     ANHGMSLPALDRATVTALAEAARRRGLLTVAHVSALWSARLALDSGVDMLTHLPLEAAL
                     DEAFVSRIADERRVVIPTLAMLEASAPKTSASARAGRSLADDPRIAGYLPDHARTAMAE
                     GHEGLCVQHASADHDFGHALSSVAALRGAGVPVLAGTDTNYRPDRACPVVHGASLHTEL
                     ALLVRAGLTPAEALTAATSAPAEHFGLFDRGMIAPGRRADLVLVEGDPTKDIGRTRSIE
                     AIWCVGVRFDREAYRKRTA"
     gene            complement(4096..5013)
                     /locus_tag="SMF913_RS30810"
                     /old_locus_tag="SMF913_26070"
     CDS             complement(4096..5013)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /locus_tag="SMF913_RS30810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMF913_26070"
                     /product="UbiA family prenyltransferase"
                     /protein_id="WP_102936291.1"
                     /transl_table=11
                     /translation="MAELSDVLTASRVPARLGRFVLRMFRPQIYVTYGVLWTLALEGSA
                     EALSGSAAHWTPSWPTVVRAVSVVLALLFARMVDEQKDLEYDRTHNPDRPLVTGAITAA
                     ELRGAMAFSTVVVVVLNTFVSAFSVLLILLALGYTLFLVALERRFPRLGERLIANLLVS
                     YPVQLLLSGYLYVSLLTGDEIGGDGRAAPLMAMFACAFLQFEFARKTEWRRDPRARLYS
                     EVLGPRGSAAVSLALAVGAVVLGLLLFGARGWPPALSIVFPALGAWRFLVRRKPSGLML
                     PAMGFVLFFNLLCFYGSTAIAIGW"
     gene            complement(5001..5996)
                     /locus_tag="SMF913_RS30815"
                     /old_locus_tag="SMF913_26071"
     CDS             complement(5001..5996)
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608204.1"
                     /locus_tag="SMF913_RS30815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMF913_26071"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_102936292.1"
                     /sec_met_domain="AfsA (E-value: 6.2e-19, bitscore: 65.9,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDTVEFSQTVPRELVHRLSLAEVFLTGVLARDEDTLDIGVQIPRS
                     HVFYRDTAGCRHIYDPMVLVEAVRQSLLMLGHDLFERPFGTKFILRDIALHGADITALA
                     VRDVPTDVVLRCRIVRRFRDREGLKGARLSYSALIGDRVAATLEGSMSWLTPAEWRAAR
                     EEGRATLELPVQATFTACESGPRIGEALVDRRDTSNVVISPPECHLTPDGGETRTARLL
                     VDTSHPVLFDHPLDHVPGMLTLEAFRQLSIATAVQTGALPSASAVLTGLKARFTGFGEL
                     DLPITCESVQAEGDGGSAVLRCAMKQAGRTIADGELRLTRAPAEAGRWRS"
     gene            complement(6012..7016)
                     /locus_tag="SMF913_RS30820"
                     /old_locus_tag="SMF913_26072"
     CDS             complement(6012..7016)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984548.1"
                     /locus_tag="SMF913_RS30820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMF913_26072"
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="WP_102936293.1"
                     /transl_table=11
                     /translation="MTEAPMDDRPFGDAISPALSRDPRKRTLFGSDTYFLESVEQLGTL
                     TADVDGFLHRHAALLLKPDAVVSRQLPTTVDWLARNGFRIVAAERTRLTRTVVRSLWYF
                     QWNLATPERRRLADLFTDSCDSVVLIVRQDADGAEALPSAPASVVLTERKGPTDPRARV
                     PGQLRHETGRYSYLLNLVHTPDEPADVARELGIYFDSGLRRRVYTSALAGEDLSVRARE
                     LGERLHAEVPERDLTFEPAAERLERAVAEAEESLAPGAVRDELRQARLAARDREGHRRL
                     LEAVWRARLPLDAWDMVIVGSHVLPMKRTGFAPVLGGVSVADWHQHQARPAVR"
     gene            complement(7013..8185)
                     /locus_tag="SMF913_RS30825"
                     /old_locus_tag="SMF913_26073"
     CDS             complement(7013..8185)
                     /NRPS_PKS="Domain: NAD_binding_4 (8-232). E-value: 3.7e-50.
                     Score: 162.3. Matches aSDomain:
                     nrpspksdomains_SMF913_RS30825_NAD_binding_4.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NAD_binding_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608202.1"
                     /locus_tag="SMF913_RS30825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMF913_26073"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_102936294.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 3.3e-17, bitscore:
                     60.5, seeds: 37, tool: rule-based-clusters)"
                     /sec_met_domain="NAD_binding_4 (E-value: 2.7e-48, bitscore:
                     162.3, seeds: 102, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRRRPRVLVTGAAGLVGAEVTARLAAAGHQVTALVHNNPVLVRND
                     GTALETGAVTCLPGDVTEPRLGLPEAEYRALAAGGLDRIVHCAAVTDFGLPDEVYQAVN
                     VDGTAHVLELARAGGVPLVHVGTAYVCGERDGLIRETELDEGQRLANAYEDSKFRAETL
                     VHKAQAGGVRAAIVRPSIVVGDERTGVVRDYKNIYVVLKLATEGKVRSIPGQYEARLDL
                     VPVDYVADIITEVTQRFEEAAGRTFHAVGAPLTLRDFSDVMAEYPPFHVPRFIPPTSFD
                     TQRLPRRERVYYERVMTLYESYFRRRMRFDDSHTAGLVTRRPPGDGQDFLRTLIDNCLE
                     TGYLGVPMADATEVLARLNITGPNATAAAHATGAPHGTGSTVSDANGVGR"
     aSDomain        complement(7490..8161)
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SMF913_RS30825_NAD_binding_4.1"
                     /evalue="3.70E-50"
                     /label="SMF913_RS30825_NAD_binding_4.1"
                     /locus_tag="SMF913_RS30825"
                     /protein_end="232"
                     /protein_start="8"
                     /score="162.3"
                     /tool="antismash"
                     /translation="VTGAAGLVGAEVTARLAAAGHQVTALVHNNPVLVRNDGTALETGA
                     VTCLPGDVTEPRLGLPEAEYRALAAGGLDRIVHCAAVTDFGLPDEVYQAVNVDGTAHVL
                     ELARAGGVPLVHVGTAYVCGERDGLIRETELDEGQRLANAYEDSKFRAETLVHKAQAGG
                     VRAAIVRPSIVVGDERTGVVRDYKNIYVVLKLATEGKVRSIPGQYEARLDLVPVDYVAD
                     II"
     gene            complement(8182..8469)
                     /locus_tag="SMF913_RS30830"
                     /old_locus_tag="SMF913_26074"
     CDS             complement(8182..8469)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="SMF913_RS30830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SMF913_26074"
                     /product="acyl carrier protein"
                     /protein_id="WP_141729439.1"
                     /sec_met_domain="PP-binding (E-value: 1.2e-05, bitscore:
                     23.7, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSSTGTHLLNDDATRNTVYAIVGAMAPAGGVHLADDTELITDLA
                     YDSLRLIELTLALEDGFGLAELGQEKTATVTTVGDIVRLVTEARHEEEGR"
     gene            complement(8486..10141)
                     /locus_tag="SMF913_RS30835"
                     /old_locus_tag="SMF913_26075"
     CDS             complement(8486..10141)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608201.1"
                     /locus_tag="SMF913_RS30835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMF913_26075"
                     /product="hypothetical protein"
                     /protein_id="WP_102936295.1"
                     /transl_table=11
                     /translation="MILSEDHLQNLLDQTIPRIREAANCAVVLEGSIAEGFGNTSSDID
                     FLLIADSDADLPTMPSLLFLDGRRVEVRTRSVRQLTEQFSAVTSVTHGHPGALSEDLLN
                     RCQRLLRSFPLRNPDLVAKVKGLMSFEDFQDTMREWWAHHARQSIRHALALRELGQDEE
                     AAAWTEAGLLQAVKSWAAGRGETYLEPKWLPMQLDRMGDQPLCARYRELASLDASGLGP
                     AEYIAAGARLTAELGVAGAEPDSERVTVARAGGVTTWPTGDRVHVVRDKQDVFVLGDGA
                     ARAWRSVVFGRPLRSVLAAAEASGAPQAGPLIAQFLRFGLVKAAWKGEGPIVPAMPLAA
                     PSGAVTPPPSTARPIVTVGGAAAGGERAIDLVPMPARRFSAAAMTLVWSNVLVENARED
                     LIGALDREQWSVAELSARRMLRAALRGVLSAYGVNPLPPDSEVVRRLSLLPAGAATDEI
                     RPKARYLETLAIASAAQGGAALTALDDFVALVRHTTGAHGFPSSFDSSDGWRQTLEIGY
                     DWLRLGAHLDADLPIDEASDLLASGGAQPHLATT"
ORIGIN
        1 tggatgaacc gctgcaacac cttcatggtc gtcaggtact cctcgtcgga gatgcccgcg
       61 cggcgttccg cctccagttc gccctggagg gccgcggcct tgtcgtagaa cgccctgccc
      121 tccgccgtga tgcccagacg cccctcggcg tcctcggtga tccagccccg ggcgatggtc
      181 gcgtcgatct ccgactccat gacgtccgca cccgagtcca gatagttgcg gaggaggcgg
      241 gacacctcac cgcgggtttt ggcggtgtcc gcgttcgcga tctgcgccag gacccaccac
      301 tgcggctggg tggtgccgat ctcggcgaga gtggactgga tgcgagtgac aacggccttg
      361 taggccgccc aactccagta gccgatgggc tgccgtagca gttcggcgtc gctgtgtgag
      421 tactccatga ccggtccctc cgttatggcc tgaactcgtt tgaacgcgtg tgaactcgct
      481 tggcgtcgag accgaccgta aaagttcaac ccgacttcaa gtcaagcccc attaggctcc
      541 tcgcatgccc gtgcgtatgg agtcttcgag ggtggcccgg tgtcttcggc cggtggtgcg
      601 ctggtggcgc tcgcggggcc tggtgacccg ggaccgtgag ctcgcgctcg tcctcacggc
      661 gctggtgttc gtcccggccc tgtcgcgtat ctcctcgacg ttcggcgatc tgcccgctcg
      721 gcacggcgat gccttcgcgg tgctcctgtc cctggggcag accgtgccgc tggtggtgcg
      781 cacccgccgg ccggccgtgt gcctggcggt ggtggggccg tcgttcgcgg tccatgagtc
      841 gctcggctat ccgcccacct tcggcagtat ggggctctac ctcgcgctgt acggcgccgg
      901 atcgcagctg tcccgccacc ggaacgtggt ggcggccctg gcgagcgcgg gctacctggc
      961 gttcgcgatc accctgcaca cgctggactc ccccgaacgg ttcctcgact acctcatctt
     1021 cttcctcgcg ctcgccgcgt tctggctcct cggcggctac gtccgtgccc ggcgtgccga
     1081 cgaggccgag cggcggcgtc tcgccgcgct cgcggccacc gcggcggaac ggtcccggat
     1141 cgcacgcgag ctgcacgacg tggtgaccca ccatgtgacc gccatggtgg tgcaggccga
     1201 cgccgcgcag ttcctcaccg aatcgcccgg ccgtgtcacc gagggactga ccgcgatcag
     1261 cggcaccggc cgccaggcgc tgggcgaact gcggcatctg ctgggcgtgt tggaggcgac
     1321 gggcacgtcg gcgcccacgc ggcggacacc cgcgctcggc accatcgagg agctcgtcgc
     1381 gcggacccgg ctcggcggtc agccggtcga actcgtcgag cagggcgaac gaccgcccat
     1441 ggcggccgga gccgaactgg ccgcctaccg ggtcgtacag gagtcgctca cgaacgccgt
     1501 caagcacgcc gccggacaca cgaccgaggt atgggtccgc taccaccgcg acggggtgga
     1561 catcgacgtg accacggacg ggccgtcccg tgtcgcccac gctccggacg ggcgcgggct
     1621 caacggactt cgagaacggg tgggcctggt cggtggcgag ctggaggcgg gcgcacggcc
     1681 cggcggcggg ttcagcgtca gcgcccggat tcccgccggg agcggctcat gatccgggtg
     1741 ctgatccgtg cgggtcggtg cgccccgcca gccgggatgc cgggacccga cggcgatgtc
     1801 gctgtggatc ggcgtcccgg ggaatgtcgc tgcccatcgg cgccgcgggg aagacgagcg
     1861 gtcagcggtc ggccgccaga agacgcacgc ggcccgtgcc cgggagggcc gggtcgaagg
     1921 ccggggggac ggggcgctca tgccgcttcg ccaggtcggt gaggagctct tcctcggcgc
     1981 tccagccgtg gtccgcgagc cactcggcgg gcggctgcgc catctcgttc ttccacaggc
     2041 cggacagcat gttcatgata cctcccggtg aggccgccgc tgcctcctgg gcctggtccg
     2101 tgttcaccat cgaccgggtg acatgctcca ccgcgaggtg gctgcccggc gcggacagcg
     2161 cgccgatccg gtcgagcagg tgatccgcgt cgcgcgcggt gaggtagacc agtatcccct
     2221 cgaccagcca ggccgtcggc tcgtccgcgc ggaagccgtg cttcatcagc agcgcgggcc
     2281 agtcctcgcg cagatccgcc gcgatcacct cgcgctcaca gcgcggcact tcgccctgga
     2341 gtacgtgttc cttgaagtcg agcacctcgg gctggtccag ttcgaagagg cgtgtcccgg
     2401 ccggccagtg caatcggaag gcacgggtgt ccagaccggc cgcgagcagg accgcctggc
     2461 ggcagcccgc cgcgcacgcc tgcatgaagt agtcgtcgaa gtaccgggtg cgcagggcga
     2521 agtagtcgcc catggcgtcg gtgaacagcc tcccctcccc ctgggtccag gccgcctgct
     2581 gctcggcgcc cgcggcgtcg acgaaacgcg tggccagggg gtcctcgaac agccggtcct
     2641 ggcgccggct ctcggccgcg cgggttatgc ccacgaacag cgcggtgctg ctgacccccg
     2701 ccaggcgctc gtgtccgtcg gtcacgtggt tctccccttc gtatggtttc ctgtcatacc
     2761 gcttccgctc atgggcgggt cgctcctgtc catgagcgtg tggtcctgtt gatgggcgtg
     2821 tcgctcccgc tcatcaggca gtgcgtttgc ggtacgcctc gcggtcgaag cgcacgccga
     2881 cgcaccagat cgcctcgatc gaccgggtgc ggccgatgtc cttcgtgggg tcgccctcga
     2941 cgaggacgag gtccgcgcgc cgccccggcg cgatcattcc gcggtcaaag aggccgaagt
     3001 gttcggcggg ggccgaggtc gccgccgtga gcgcctccgc cggagtcagt ccggcccgga
     3061 cgaggagggc gagctcggtg tgcagactgg cgccgtggac gaccggacag gcacggtccg
     3121 gccggtagtt ggtgtcggtt cccgccagta cgggcacacc tgccccgcgc agagcggcca
     3181 cgctcgaaag ggcgtggccg aagtcgtggt cggcggacgc atgctgcacg cacagaccct
     3241 catggccttc cgccatcgcc gtacgggcgt gatccgggag gtagccggcg atccgcggat
     3301 cgtccgccag tgaccgtcct gcgcgcgccg aggcggaggt cttgggtgcg gacgcctcca
     3361 gcatggccag ggtggggatg acgacccgcc gctcgtccgc gatccggctg acgaacgcct
     3421 cgtccagagc cgcttccagc ggaaggtgtg tcagcatgtc cacgccgctg tccagggcga
     3481 gccgtgcgga ccacagggcg gacacatggg ccacggtgag cagaccgcgg cgccgcgcgg
     3541 cctcggcgag cgccgtcacg gtcgcccggt ccagagcggg cagcgacatc ccgtggttcg
     3601 cgccgtcatc cacgatgatc ttgatgaagt gtgcgccctc cgcctgcctc tcggccacga
     3661 acgcatcggc ccgctcgggc ccggccaccg tcggcagtag cggcatggtg acgccggggt
     3721 gcccaccggg cgcgctcgcc accagccccg agctgcgcat atcggcgagg tcggcacgct
     3781 cgccggccgc cgcgcacagg gcctgggtgg cctccggcgg gcccatgaac atgtccagtt
     3841 cggttgtcac accgaaggcc agcgccagtt cgagattgct ctcggcgccg aacacatgcg
     3901 tatgcgcgtc gatcagcccc ggcagcagcg tcatccccgt tccgtcgatc acatcggccg
     3961 acgccggtgc caccggggcg ggttcggtcg tgacacgtgt gatgaccccg tcggcgaact
     4021 ccaccgagcc ggggcccagc atcgctctcc cgtcgaagat ccgcaccttg tcgatgatca
     4081 ccggccgcat ccctttcacc atccgatcgc gatcgctgtg gacccgtaga aacacagcag
     4141 gttgaaaaag agcacaaagc ccatggccgg gagcatcagc ccggatggtt ttcggcggac
     4201 caggaagcgc cacgccccca gcgcggggaa gacgatgctc aacgcgggcg gccacccccg
     4261 tgccccgaac agcagcagcc cgaggaccac cgcgcccacg gcgagggcga gggagacggc
     4321 agcgctgcca cgcgggccga gcacctccga gtacagccgt gcccgcgggt cccgccgcca
     4381 ctcggtcttc cgcgcgaact cgaactggag gaaggcacac gcgaacatgg ccatgagagg
     4441 ggcggcccgc ccgtcaccgc cgatctcgtc gccggtcagc agcgagacat agagatagcc
     4501 ggagagcagc agctgcaccg ggtagctgac gaggagattg gcgatgaggc gctcgccgag
     4561 ccgtgggaac cggcgctcca gcgccaccag aaacagcgtg tatccgaggg cgagcaggat
     4621 cagcagcacg gagaaggccg agacgaacgt gttgagcacg acgacgacca ccgtgctgaa
     4681 cgccatggcg ccgcgcagct cggccgccgt gatggcaccc gtcaccagcg gccggtccgg
     4741 gttgtgcgtg cggtcgtact ccagatcctt ctgctcatcg accatacggg cgaacagcag
     4801 ggccagcacc acgctcaccg cgcgcaccac ggtcggccag gagggcgtcc agtgcgcggc
     4861 ggacccggag agcgcctcgg ccgagccctc cagggccagg gtccacagca caccgtaggt
     4921 gacatagatc tgcgggcgga acatccgcag aacgaagcgg cccaggcgtg ccggtacccg
     4981 ggaagcggtc aggacatcgc tcaactccgc caccgacccg cttccgcggg ggcccgtgtg
     5041 aggcgcaatt cgccatcggc gatggtgcgt ccggcctgct tcatggcgca gcgcagaaca
     5101 gcgctcccgc cgtcgccttc ggcctgaacg ctctcgcagg tgatcggcag atccagctcg
     5161 ccgaagcccg tgaagcgcgc cttcagcccg gtgaggacag ccgacgccga cggcagtgcc
     5221 ccggtctgca cggcggtggc gatgctcagc tgccggaacg cctcgagggt cagcatgccg
     5281 gggacatggt ccagggggtg gtcgaacagc acggggtggc tggtgtccac gaggagccgt
     5341 gcggtcctcg tctctccccc gtcgggcgtg aggtggcact cgggcgggga gatgaccacg
     5401 ttcgacgtgt ccctgcggtc gaccagggcc tccccgatcc gcggccccga ctcgcaggcc
     5461 gtgaacgtgg cctggacggg cagctccagc gtggcccgcc cttcctcgcg cgccgcccgc
     5521 cactcggcgg gcgtcagcca ggacatggag ccctccagcg tcgccgccac gcggtcgccg
     5581 atcagcgcgc tgtaggacag ccgcgcgccc ttcaggccct cgcggtcacg gaagcggcgg
     5641 acgatacggc agcgcagcac cacatcggtg ggtacgtcgc gtaccgcgag cgcggtgatg
     5701 tccgcgccgt gcagggcgat gtcccgcagg atgaacttcg tgccgaacgg cctctcgaac
     5761 agatcgtggc ccagcatgag gagcgactgc cggaccgcct ccaccaggac catcgggtcg
     5821 tagatgtggc ggcagcccgc cgtgtcccgg tagaagacgt gcgagcgggg tatctgcacc
     5881 ccgatgtcga gggtgtcctc gtcccgggcg agcacgccgg tcaggaacac ctcggcgagg
     5941 ctcagccggt ggacgagctc ccgcggcacc gtctggctga attccaccgt gtccatgctt
     6001 ccccctccgt attaccgaac cgccggccgc gcctggtgct gatgccagtc ggccacgctg
     6061 accccgccga gtacgggagc gaatcccgtg cgcttcatcg gcaggacatg agacccgacg
     6121 atcaccatgt cccaggcgtc gagcggcagc cgggcccgcc agaccgcttc gagcagccgc
     6181 cggtggccct cccggtcccg ggcggcgagc cgggcctggc gcagttcgtc ccggaccgcg
     6241 ccgggggcca gggattcctc cgcctccgcc acggcccgct cgagccgttc cgcggccggt
     6301 tcgaaggtga gatcccgctc gggcacctcg gcgtgcaacc gctcgcccag ctcacgcgcc
     6361 cggaccgaca gatcctcgcc cgccagtgcg gaggtgtaga cccgccgccg gagcccggag
     6421 tcgaagtaga tgcccaactc ccgtgccaca tcggctggtt cgtccggggt gtggacgagg
     6481 ttgagcagat agctgtagcg gccggtctcg tggcggagct ggccggggac gcgggcccgt
     6541 gggtcggtcg ggcccttccg ctcggtcagg acgaccgacg cgggtgcgct cggcagagcc
     6601 tcggccccat cggcgtcctg gcgcacgatg agcaccaccg aatcacagga gtcggtgaac
     6661 agatcggcga gacggcggcg ctccggagtg gccaggttcc actggaagta ccacagcgac
     6721 cgcacgacgg tacgggtgag ccgggtgcgt tcggcggcca cgatgcggaa gccgttcctg
     6781 gccagccagt cgaccgtcgt cggcaactgc ctgctgacca cggcatccgg tttgagcagc
     6841 agcgcggcgt gccggtggag aaagccgtcg acatcggcgg tgagggtgcc gagttgctcg
     6901 acgctctcca ggaagtaggt gtccgagccg aagagggtgc gctttcgcgg atcgcggctg
     6961 agggccgggg atatggcgtc gccgaagggc cggtcgtcca tcggggcctc ggtcatcggc
     7021 cgactccgtt cgcatcgctc acggtcgagc cggtcccgtg tggcgcgcca gtggcgtgtg
     7081 ccgcggcggt cgcgttcggc cccgtgatgt tcagccgggc gagcacctcc gtggcatcgg
     7141 ccatcggtac gcccagatag cccgtctcca ggcagttgtc gatcagtgtg cgcaggaagt
     7201 cctgcccgtc cccgggtggt ctgcgggtga ccaggcccgc ggtgtgggag tcgtcgaaac
     7261 gcatccggcg ccggaagtag ctctcgtaga gcgtcatcac gcgctcgtag tacacgcgtt
     7321 cgcgccgggg cagccgctgg gtgtcgaagc tggtcggagg gatgaaccgc ggcacgtgga
     7381 acggggggta ctcggccatc acatcggaga agtcccgcag cgtcagcggt gcgcccaccg
     7441 catggaaggt gcggcccgcc gcctcctcga accgctgggt gacctcggtg atgatgtccg
     7501 ccacgtagtc gacgggcacc aggtcgagac gggcctcgta ctggccgggg atggagcgga
     7561 ccttgccctc ggtggccagc ttgaggacga cgtagatgtt cttgtagtcg cgcaccacgc
     7621 cggtgcgctc gtcgccgacg acgatactgg ggcggacgat cgcggctcgc accccgccgg
     7681 cctgggcctt gtgcaccagg gtctcggccc ggaatttgct gtcctcgtag gcgttggcca
     7741 ggcgctggcc ctcgtccagc tcggtctcgc ggatcagccc gtcgcgttca ccacacacat
     7801 aggccgttcc cacatgtacg agggggacgc cgccggcgcg ggccagttcg aggacatggg
     7861 cggtgccgtc gacgttgacg gcctggtaga cctcgtcggg gaggccgaag tcggtgaccg
     7921 cggcgcagtg cacgatccgg tcgaggccac cggcggccag cgcccggtat tcggcctcgg
     7981 gcaggccgag ccggggctcg gtcacatcgc cgggcaggca ggtgacggca cccgtctcca
     8041 gcgcggtgcc gtcgttgcgc accagcaccg ggttgttgtg gaccagggcg gtgacctggt
     8101 gtccggcggc ggccagccgg gcggtgacct ccgcgccgac cagcccggcc gcaccggtca
     8161 ccagaaccct cggacgccgc ctcaccggcc ctcctcctcg tgccgtgcct cggtcaccag
     8221 gcgcacgatg tcgccgaccg tggtgaccgt ggcggtcttc tcctggccga gttcggcaag
     8281 tccgaagccg tcctccagtg ccagcgtcag ctcgatgagc cggagcgagt cataggccag
     8341 atccgtgatc agctcggtgt cgtcggccag atggacgccc cccgcgggcg ccatcgcgcc
     8401 gacgatcgcg tacaccgtgt tccgcgtcgc gtcgtcgttc agcaagtgtg tgccggtgct
     8461 tgacgtcaac gttcctccct cagcgtcagg tggtggcgag gtgcggctgt gcgcccccgc
     8521 tggccagcag atcgctcgcc tcgtcaatgg gcaggtccgc gtcgaggtgt gcgcccaggc
     8581 gcagccagtc gtagccgatc tccaaggtct gccgccatcc gtccgaggag tcgaaggagg
     8641 acgggaagcc gtgcgccccg gtcgtgtgcc ggaccagtgc gacgaagtcg tccagcgccg
     8701 tgagcgccgc gccgccctgg gccgcggacg ctatcgcgag cgtttcgagg taccgggcct
     8761 tcggccggat ctcatcggtg gccgcccccg ccggaaggag ggagagcctg cggacgacct
     8821 cggagtccgg tggcagcggg ttgacgccgt acgcgctgag cactccgcgc agcgcggccc
     8881 gcagcatcct ccgggcgctg agttcggcga cgctccactg ctcacggtcc agggcgccga
     8941 tgaggtcctc gcgggcgttc tccaccagca cgttggacca caccagggtc atggccgcgg
     9001 cgctgaaccg gcgtgcgggc atgggtacga gatcgatggc ccgctcaccg ccggccgccg
     9061 ccccgcccac ggtgacgatg ggtcgcgcgg tgctcggcgg cggtgtgacg gcgccggaag
     9121 gggccgcgag cggcatggcg gggacgatcg ggccttcgcc cttccaggcg gccttcacca
     9181 ggccgaaccg gaggaactgg gcgatcagcg ggcccgcttg cggtgcccct gacgcctccg
     9241 ccgcggccag cacactgcgc agcggccggc cgaagacgac ggaacgccag gcccgcgccg
     9301 ccccgtcgcc gaggacgaac acgtcctgct tgtcgcgtac gacatgcacc cggtcgccgg
     9361 tcggccaggt ggtcacccca ccggccctgg ccacggtgac ccgctcggag tcgggctcgg
     9421 ctcccgccac ccccagctcc gcggtgagcc gggcgcccgc ggcgatgtac tcggcggggc
     9481 cgagacccga cgcgtcgagg gaagcgagct cccggtaccg ggcgcacagc ggctggtcac
     9541 ccatgcggtc cagctgcatg ggcagccact tcggctccag ataggtctcc ccccgccccg
     9601 ccgcccagga cttcacggcc tggagcaacc cggcttcggt ccatgccgcg gcctcctcgt
     9661 cctggccgag ttcgcgcagg gcgagcgcgt ggcggatcga ctggcgggcg tggtgtgccc
     9721 accactcgcg catcgtgtcc tggaaatcct cgaaggacat cagccccttg accttcgcca
     9781 ccaggtccgg attgcgcagc gggaagctgc gcagcaggcg ctgacagcgg ttcagcaggt
     9841 cttcggagag cgcgcccggg tggccgtggg tgacggacgt gaccgcggag aactgctcgg
     9901 tgagctgccg tacggagcgg gtcctgacct cgacccgccg cccgtccagg aacaggagcg
     9961 agggcatggt gggcaggtcg gcgtcgctgt cggcgatgag caggaagtcg atgtcggacg
    10021 atgtgttgcc gaagccctcg gcgatcgaac cctccaggac cacggcacag ttggcggctt
    10081 cccggatccg cgggatggtc tggtcgagca gattctgtag atggtcctcg ctgaggatca
    10141 cacgggcctc ccggtcgtga catgggccgt cccgaggacg cgtacgtctt tgagacggcc
    10201 cgctcgcagc tgttcccaca tgtggcgacg tctcggcttg ccgctggacg tctttctgat
    10261 cagcccgctg tgtccgatga cgacggtgat ggcgatgtcg tcgccgagcg cgccgcgcag
    10321 ggcgaccgtg gcgtcctcca cccacgcgcc ctcggccttc tccgcgaaca gcgtcaccga
    10381 ggtgccaccg gtgccctcga cgccgacgac cgcgcacctc tcccgggcga tcccggtggc
    10441 ggagaccacc ttggcctcca ggtcctccat gtagacgctg cggccgcgtg ccttgaggct
    10501 gtcgcccatc cggccgagca cgaacagctc gccacggtgg aagaacccgg cgtccccggt
    10561 cctgatgccg ccttcggtga accgcgtgga gccggtgcgg ccggcgtggt agccactggt
    10621 gaccgagggg ccggtcacct cgatctcgcc gaggtgaccg tccggcaggg gccgtccccc
    10681 gtcgtcgacc accgtggcgc ccaccccgtc gccggggccg ccgcagccga tcagccagcc
    10741 gtcgccgtcc ccttcaccat ccctgcccac ttcgccatcc ccgccgcctt cgccgtcgcc
    10801 gtcggagggc gtatgcgcgg agctcgcgaa ctcgccctcc ccgtcgatgg cgacgggccg
    10861 cccgaagcgc agcgagcgcg tgtccacgcg tacggtcggc gcgggagtgc cggaggtacg
    10921 tacggtcacc gcgagcgtcg cctcggcgag cccgtacgac ggaaggtaga cggccgggga
    10981 gaatccggcg ggcgct
//
