LOCUS       NZ_LJIW01000001        10405 bp    DNA     linear   CON 11-JUN-2024
DEFINITION  Streptomyces malaysiensis strain F913 SMF9131, whole genome shotgun
            sequence.
ACCESSION   NZ_LJIW01000001
VERSION     NZ_LJIW01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces malaysiensis
  ORGANISM  Streptomyces malaysiensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces violaceusniger group.
REFERENCE   1  (bases 1 to 6203920)
  AUTHORS   Xu,Y., Wei,J., Xie,J., Li,T. and Zhou,Z.
  TITLE     Genome sequence, genome mining and natural product profiling of a
            biocontrol bacterium Streptomyces malaysiensis F913
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6203920)
  AUTHORS   Li,T. and Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2015) State Key Laboratory of Silkworm Genome
            Biology, Southwest University, Tian Sheng Road, Beibei, Chongqing,
            Chongqing 400715, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-FEB-2015
            Assembly Method                   :: SOAPdenovo v. 2.04
            Expected Final Version            :: yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002891865.1-RS_2024_06_11
            Annotation Date                   :: 06/11/2024 05:20:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,419
            CDSs (total)                      :: 9,336
            Genes (coding)                    :: 9,091
            CDSs (with protein)               :: 9,091
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 6, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 59 of 245
            Pseudo Genes (incomplete)         :: 218 of 245
            Pseudo Genes (internal stop)      :: 14 of 245
            Pseudo Genes (multiple problems)  :: 43 of 245
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:22:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3223360
            Orig. end                         :: 3233765
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LJIW01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="19"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5405
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(862..875)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.11"
                     /tool="antismash"
     gene            1333..1935
                     /locus_tag="SMF913_RS12710"
                     /old_locus_tag="SMF913_12490"
     CDS             1333..1935
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009718917.1"
                     /locus_tag="SMF913_RS12710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMF913_12490"
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="WP_250850706.1"
                     /transl_table=11
                     /translation="MARLAHLLLGSSDGADDLAADALVAVWHQWDRVRAADHPAAYARG
                     VVANLARSRIRSLVRERRRVALFSSQRSDHVDDPDVSAVVDVQAALVRLPFRKRACVVL
                     RHAFDLSERETARTLGISVGTVKSQTSRGIAELERLLGPPDGEGELPGATDAGRTVRTL
                     RSGNTGPTGTGPTGTSPAGPARHSGQMGHAGHAGGGS"
     gene            1935..2747
                     /locus_tag="SMF913_RS12715"
                     /old_locus_tag="SMF913_12491"
     CDS             1935..2747
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014059804.1"
                     /locus_tag="SMF913_RS12715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMF913_12491"
                     /product="hypothetical protein"
                     /protein_id="WP_102934232.1"
                     /transl_table=11
                     /translation="MIEHRERAEGRDVVAELRAAAASHEPNRSRMLARVTAGMAADDRR
                     RSRDPRMGPRRPRMVGPVAGLAAAVAATGLVVLATDGAERPQTVRTSSEPVVPPAGAGA
                     DTRPGEEPGAGSQSEAERHTPSAYPTAAGPNHLAEPTVRSKGAINAHSNPYWAQSDITL
                     TIGRPLTSLTVELRVAETGGVRPTGSWSSLPTSDMTAATHIEDGALVYRWTLRKGATVP
                     AGRFVFAGQYNHAEGERDNGRDAYSASGNGDSGAFAVRGDFPRFTVGD"
     misc_feature    2822..2835
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.61"
                     /tool="antismash"
     misc_feature    2831..2846
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 21.4"
                     /tool="antismash"
     misc_feature    complement(2895..2910)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.52"
                     /tool="antismash"
     misc_feature    3031..3043
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 18.12"
                     /tool="antismash"
     gene            3068..3628
                     /gene="ectA"
                     /locus_tag="SMF913_RS12720"
                     /old_locus_tag="SMF913_12492"
     CDS             3068..3628
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014059805.1"
                     /locus_tag="SMF913_RS12720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMF913_12492"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_102934233.1"
                     /transl_table=11
                     /translation="MTAAQADLVRSELDLPEDLTLDTPRVEDGAAIWRIARDSKALDLN
                     SSYSYLLWCRDFAATSVVARDADGGPVGFITGYVRPERPETLVVWQVAVDHARRGRGLA
                     ATLLDGLVKRVAATGVRGIETTITPDNTASNRLFTSFAERHQAPLEREVLFHGGLFPDG
                     AHEPEVLYRIGPLGSLGPSTDRT"
     gene            3698..4960
                     /gene="ectB"
                     /locus_tag="SMF913_RS12725"
                     /old_locus_tag="SMF913_12493"
     CDS             3698..4960
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-363). E-value: 2.7e-72.
                     Score: 235.6. Matches aSDomain:
                     nrpspksdomains_SMF913_RS12725_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="SMF913_RS12725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMF913_12493"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_093700080.1"
                     /sec_met_domain="Aminotran_3 (E-value: 7.2e-76, bitscore:
                     253.6, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFEALESEVRSYCRGWPAVFDRAQGSHMYDEDGHTY
                     LDFFAGAGSLNYGHNNPVLKRALIDYIERDGVTHGLDMSTTAKRAFLESFQNHILRPRD
                     LPYKVMFPGPTGTNAVEAALKLARKVKGREAIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGTIPDFLWFERLLEDQGSGLNRPAAVIVETVQGEGGINVARP
                     EWLRALAELCERQDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTVSKSISGYGMPMS
                     LALFKPELDIWEPGEHNGTFRGNNPAFVTAAATLDTYWADGQMEKQTLARGEQVELALR
                     AICEEQSGTQFRGRGLVWGLEFDDKPRATAVCRRAFELGLLLETSGPESEVVKLLPALT
                     ISPDDLDEGLRTLARAVRETA"
     aSDomain        3782..4786
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SMF913_RS12725_Aminotran_3.1"
                     /evalue="2.70E-72"
                     /label="SMF913_RS12725_Aminotran_3.1"
                     /locus_tag="SMF913_RS12725"
                     /protein_end="363"
                     /protein_start="28"
                     /score="235.6"
                     /tool="antismash"
                     /translation="FDRAQGSHMYDEDGHTYLDFFAGAGSLNYGHNNPVLKRALIDYIE
                     RDGVTHGLDMSTTAKRAFLESFQNHILRPRDLPYKVMFPGPTGTNAVEAALKLARKVKG
                     REAIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGTIPDFLWFER
                     LLEDQGSGLNRPAAVIVETVQGEGGINVARPEWLRALAELCERQDMLLIVDDIQMGCGR
                     TGAFFSFEEAGITPDIVTVSKSISGYGMPMSLALFKPELDIWEPGEHNGTFRGNNPAFV
                     TAAATLDTYWADGQMEKQTLARGEQVELALRAICEEQSGTQFRGRGLVWGLEFD"
     gene            5001..5405
                     /locus_tag="SMF913_RS12730"
                     /old_locus_tag="SMF913_12494"
     CDS             5001..5405
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009718913.1"
                     /locus_tag="SMF913_RS12730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMF913_12494"
                     /product="ectoine synthase"
                     /protein_id="WP_069869916.1"
                     /sec_met_domain="ectoine_synt (E-value: 2e-53, bitscore:
                     177.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEDTERHVRSKSGTWESKRIVLAKEGVGFSLHETILY
                     AGTETSMWYANHIEAVLCVEGEAELTNDETGEKHWITPGTMYLLDGHEKHTMRPKTDFR
                     CVCVFNPPITGREDHDENGVYPLITETEEA"
     gene            5410..6297
                     /gene="thpD"
                     /locus_tag="SMF913_RS12735"
                     /old_locus_tag="SMF913_12495"
     CDS             5410..6297
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009718912.1"
                     /locus_tag="SMF913_RS12735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMF913_12495"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_079257182.1"
                     /sec_met_domain="PhyH (E-value: 9.1e-40, bitscore: 135.3,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAVTDLYPTRGAVEVATPRVDPVVWSEPGAPGPLQPSELSDFDR
                     DGFLAIDELITPDEVAEYRAELDRMIADPDVRADERSIIEPTSQDVRSVFEVHRISEVF
                     AKLVRDPRVVGRARHILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPNMRT
                     VSVSVALTENFAHNGSLMIMPGSHRTFLGCAGATPKDNYKKSLQMQDAGTPSDEALSGF
                     ADKHGIRQFTGKPGSAVWFDCNCMHGSGDNITPFSRSNVFIVFNSVENTAVEPFAAPVP
                     RPDFIGARDFTPVR"
     gene            complement(6426..7472)
                     /locus_tag="SMF913_RS12740"
                     /old_locus_tag="SMF913_12496"
     CDS             complement(6426..7472)
                     /NRPS_PKS="Domain: Aminotran_5 (14-342). E-value: 2.3e-25.
                     Score: 81.2. Matches aSDomain:
                     nrpspksdomains_SMF913_RS12740_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014180697.1"
                     /locus_tag="SMF913_RS12740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMF913_12496"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_102934234.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.4e-19, bitscore:
                     68.5, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MESLAGAEFAPHTTYLTTASSGLIPARSVAAVQAVLDGSATGQPI
                     VDVAFEAVEAARAAYARLVGVPARRVAAGSSVAVYAGMIATSLPPGAEVLVAEGDFSSL
                     VNPFAVRGDLKLRIVPLEEIAEAVRPGTALVAVSAVQSADGRIADLDAIREAARTHGAR
                     TLVDVSQAAGWFPVNAAEADYTVCVAYKWLMCPRGAAFLTVPEDLGGLTPVFAGWVAGE
                     KPWDSCYGPVEELAHSARRFDESPSVYSYVAARHSLALIEELGTEAIAAHDRALADRFR
                     DGVIGLGHRPIAAPGSAIVSVPGLGDAAPRLAEAEVHVSARAGNLRASFHLYNTVADVD
                     RLLEKLSG"
     aSDomain        complement(6447..7430)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SMF913_RS12740_Aminotran_5.1"
                     /evalue="2.30E-25"
                     /label="SMF913_RS12740_Aminotran_5.1"
                     /locus_tag="SMF913_RS12740"
                     /protein_end="342"
                     /protein_start="14"
                     /score="81.2"
                     /tool="antismash"
                     /translation="YLTTASSGLIPARSVAAVQAVLDGSATGQPIVDVAFEAVEAARAA
                     YARLVGVPARRVAAGSSVAVYAGMIATSLPPGAEVLVAEGDFSSLVNPFAVRGDLKLRI
                     VPLEEIAEAVRPGTALVAVSAVQSADGRIADLDAIREAARTHGARTLVDVSQAAGWFPV
                     NAAEADYTVCVAYKWLMCPRGAAFLTVPEDLGGLTPVFAGWVAGEKPWDSCYGPVEELA
                     HSARRFDESPSVYSYVAARHSLALIEELGTEAIAAHDRALADRFRDGVIGLGHRPIAAP
                     GSAIVSVPGLGDAAPRLAEAEVHVSARAGNLRASFHLYNTVADVDRL"
     gene            complement(7738..9273)
                     /locus_tag="SMF913_RS12745"
                     /old_locus_tag="SMF913_12497"
     CDS             complement(7738..9273)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014059810.1"
                     /locus_tag="SMF913_RS12745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMF913_12497"
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="WP_250850555.1"
                     /sec_met_domain="Aldedh (E-value: 8.2e-42, bitscore: 141.5,
                     seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAPVWSVDPRTGKQREQVAVEATAEEVDRVVRAAHAARGSLADR
                     AVRAALLRTAADLLDEARDLLVEAADAETALGPARLTGELARTTYQLRSFATIVDEGGF
                     LDVRIDHADATQTPPWPDLRRYKLPLGVVAVYAASNFPLAFSVPGGDTASALAAGCPVI
                     VKAHPDHPATSEVAASALRRAAERVGVPAEVIAVVHGFDAGIELVRHPLIAAAGFTGSV
                     RGGRALHDAAASRPVPIPFHGELGSLNPVVVTEAAAAERGDQIGAGLAGSMTLGVGQFC
                     TKPGFVLAPAGPAGDGLVKSLTEAVSNTEAGVLLDHRMRDNFVAGVQERAELADVEAPV
                     TPGAGGEHTVSAGFLTVPAGRLAESGPHDALLEECFGPVTVVARYESDEEISAVLGRLP
                     GNLTATVHLSAAEAEGTEGRAGELIAELTPLAGRVLVNGWPTGVAVAPAQHHGGPYPAT
                     TSTSTSVGGTAIERWLRPVAYQDTPAALLPPELRDENPLELPRRINGVREQRG"
     gene            9409..10179
                     /locus_tag="SMF913_RS12750"
                     /old_locus_tag="SMF913_12498"
     CDS             9409..10179
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1195: IclR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381154.1"
                     /locus_tag="SMF913_RS12750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMF913_12498"
                     /product="IclR family transcriptional regulator"
                     /protein_id="WP_069869920.1"
                     /transl_table=11
                     /translation="MSAAETGGAQVKSAVRTVELLEFFAGRPGMHSLAAVQEAVGYPKS
                     SLYMLLRTLVELGWVETDATGTRYGIGVRALLVGTSYIDGDEVVAAARPTLDRLSDDTT
                     ETIHLARLDGTNIVYLATRQSQHYLRPFTRVGRRLPAHSTSLGKALLATYSDEQVRKLL
                     PETLSQLTEHTITDREKLIEELHLVREQGYAVDREENTLGLRCFGIAIPYRTPSRDAIS
                     CSVPVARLTPAHEQMIKDALFDARDRLTLATRRL"
ORIGIN
        1 ccatgggcca agccgccgcc cgcgtcggtg ccggtgtaga tcgggatgcc cgcgtcgtag
       61 gccgcccgca ccgtctcgta ccggcgctcg tgcagccgcc gcatatggtc ggcccagcgc
      121 gggaacctcg cctcgccgcc cagcgccagc cgcgggaagg tggcgatgtt gaccagggtc
      181 gggacgatcg ccacgccccg ctcggcgaac agcgggatgg tctcctcggt gagcccggtg
      241 gcgtgctcga tgcagtcgat ccccgcctcg accagcgggg ccaggctctc ctcggcgaaa
      301 cagtgcgccg tgacccgcgc gcccagccgg tgcgcctccg cgatggccgc ctcgaccgcg
      361 ccgcgcggcc agcaggaccc caggtcaccg gtctcccggt cgatccagtc gccgaccagc
      421 ttgacccagc cgtcgccgcg ccgcgcctcc gcggctacat aggcgaccag gtcctcgggc
      481 tcgatctcat acgcgtagtt gcggatgtag cgcctggtac gggcgatatg gcggccggcc
      541 cggatgatac ggggcagatc ctcgcggtcg tcgatccagc gggtgtcggc cggcgatccc
      601 gcgtcccgca gcagcagcgc cccggcgtca cggtcggtca gcgcctgttt ctcgctggtg
      661 gagtcgtcca ccgcgccatg ggcgtcgagg ccgacatggc agtgggcgtc gacgagtccg
      721 ggcagcaccc agccctcgat cagccggatg tccctggccc cggccggccg ctcgaacgtg
      781 atccggccgt ccaccaccca cagctcgtcc cggatgtccc cggcagcgtc gccggtgtcc
      841 ggtccgacca ggacccggcc cttgatatgg agcaccgcgc tatccctcat gcaacgcact
      901 gtacgagacc ggggcagggc gttgaccggc caattcccag gagccggaac ggcgccactc
      961 gaacaacttg cgcagcgctg ctacgctcac gcaacccacc ggacgagcgg cagtgaggag
     1021 gtcctcgtga gcgctccgac cgatgacgcg gccgccgcga gcaccgcgcg agccgtaccg
     1081 atggacacga cgcacacgac ggacccacca cgggccgcag caccggaggc gcccgacgcg
     1141 ccggagggcc ccgacggctt atcggccgac gcccaccccg acttaccggc cggctccccg
     1201 gccactgggc accccgaccc accggccgcc ccgcacgccg acttacagac cgccgccccg
     1261 caccccgacc cacccggccc acccgaccca caagccgagt tccgggactt cttcgagcgg
     1321 caccacgccg agctggcccg cctcgcccat ctgctgctcg gcagctccga cggggcggac
     1381 gacctggcgg ccgacgcgct ggtggcggtc tggcaccagt gggaccgggt gcgggccgcc
     1441 gaccatccgg ccgcgtacgc ccggggcgtg gtcgccaatc tggcccgctc ccggatccgc
     1501 agcctggtgc gcgagcggcg gcgggtggcg ctcttctcct cgcagcgctc cgaccatgtg
     1561 gacgaccccg atgtgtccgc cgtggtggat gtgcaggccg cgctggtccg gctgccgttc
     1621 cgcaagcggg cgtgtgtggt gctgcggcac gcgttcgacc tctccgagcg ggagacggcc
     1681 cgtacgctgg gcatttcggt gggcacggtc aagagtcaga cctcccgcgg aatcgccgag
     1741 ctggagcggc tgctcggccc tccggacggc gagggcgagc tgcccggggc gacggacgcg
     1801 gggcggacgg tacggacgct ccgctccggg aacacaggtc ccacgggcac aggtcccacg
     1861 ggcacaagtc ccgcgggccc cgctcgtcac tcgggtcaga tgggccacgc cgggcacgca
     1921 ggagggggca gttgatgatc gagcatcgcg agcgcgccga gggccgcgat gtcgtcgccg
     1981 agctgcgcgc ggccgccgcc tcccatgaac cgaaccggag caggatgctg gcgagggtca
     2041 cggcgggcat ggcggccgat gaccgacgcc ggagccggga cccgcggatg ggcccgcgcc
     2101 ggccccggat ggtgggcccg gtcgccgggc tggcggcggc ggtcgccgcg acgggcctcg
     2161 tggtgctggc cacggacggt gccgagcggc cgcagacggt gcgcacctcg agcgaacccg
     2221 tcgtgccccc ggcgggcgcg ggcgcggaca cacggcccgg ggaggagccg ggcgccgggt
     2281 cgcagagcga agctgagcgg cacacgccga gcgcctatcc caccgcggcc ggtccaaacc
     2341 acctcgccga gccgaccgta cggtccaagg gcgccatcaa cgcccattcc aacccttact
     2401 gggcgcagag cgacatcacg ctcaccatcg gcaggccgct gacctcgctg acggtggagt
     2461 tgcgggtcgc ggagaccggg ggtgtgcggc ccaccggctc ctggagctcg ctgccgacct
     2521 ccgatatgac cgccgccacg catatcgagg acggtgcgct ggtctaccgc tggacgctcc
     2581 ggaagggtgc gaccgtaccc gcgggccggt tcgtcttcgc ggggcagtac aaccacgccg
     2641 agggcgagcg ggacaacggc cgggacgcct actcggcctc gggcaacggt gactccgggg
     2701 ccttcgcggt gcggggcgac ttcccccggt tcaccgtggg agattgaatt catggcagct
     2761 tggccagaaa ttctccgcgt ttttccgcta ctgcttccac attcgtctgc ccgcttttgt
     2821 gattcctaaa cacaaaattt tttcacggga tcctgatccc gtaattcaag acagtcaccg
     2881 ggcgattacg cacatgtgac ggaccacaca tgagattcac tttgccccgg tatctccccc
     2941 acaaatcagc ctctttcggc gtgcggccgc gcgcacgcgc gctcccgcgt gataacacga
     3001 ccaccccctt tcgcgtaacc ccccaccgcg atgcattttc cgattctgct gggtaaattg
     3061 aatttgcatg accgccgcac aagcagacct tgtccgtagc gaattggatt tgccggagga
     3121 cctgaccctc gacaccccgc gcgtcgagga cggagccgcg atctggcgaa tcgcacgcga
     3181 ctccaaagcc ctggacctca actcctccta cagctacctc ctgtggtgcc gcgacttcgc
     3241 cgccacatcc gtagtggcgc gcgacgccga cggcggaccg gtcggcttca tcaccggcta
     3301 cgtccgcccc gagcgccccg agaccctggt cgtctggcag gtcgccgtcg accacgcccg
     3361 gcgtggccgc ggactggccg ccaccctgct ggacgggctg gtcaagcggg tcgccgcgac
     3421 gggcgtccgc ggcatcgaga cgacgatcac cccggacaac accgcctcga accggctgtt
     3481 cacctcgttc gccgagcggc atcaagcgcc gctcgagcgt gaggtgctct tccacggcgg
     3541 actgttcccc gacggcgcgc acgagcccga ggtgctctac cgcatcggtc cgctcggctc
     3601 cctcgggccg agcaccgacc ggacctgacc cccacctcga tccccctcga accctcaccc
     3661 tcctcacccc ctcacacccc tctcccagga gaacgctgtg accatcaccc cgcccgccct
     3721 gagcgtcttc gaggccctgg agtcggaggt gcgcagctac tgccgtggct ggcccgccgt
     3781 cttcgaccgc gcgcagggca gccacatgta cgacgaggac ggccacacgt acctcgactt
     3841 cttcgccgga gccgggtcgc tcaactacgg ccacaacaac ccggtgctga aacgggcgct
     3901 gatcgactac atcgagcgtg acggcgtcac gcacggcctg gacatgtcca ccacggccaa
     3961 gcgcgcgttc ctcgaatcgt tccagaacca catcctgcgc ccgcgcgatc tgccctacaa
     4021 ggtcatgttc ccgggcccga ccggcaccaa cgccgtcgag gccgccctga agctggcccg
     4081 taaggtcaag ggccgcgagg ccatcgtctc cttcaccaac gccttccacg gcatgtcgct
     4141 gggctcactg gccgtgaccg gcaacgcctt caagcgcgcc ggtgcgggca tcccgctggt
     4201 ccacggcacc ccgatgccgt tcgacaacta cttcgacggc acgatcccgg acttcctgtg
     4261 gttcgagcgg ctgctggagg accagggctc cggcctcaac cggcccgccg ccgtgatcgt
     4321 cgagaccgta cagggcgagg gcggcatcaa cgtggcgcgg cccgagtggc tgcgcgccct
     4381 ggccgagctg tgcgagcgcc aggacatgct gctgatcgtc gacgacatcc agatgggctg
     4441 cggccgcacg ggcgccttct tctccttcga ggaggcgggc atcaccccgg acatcgtgac
     4501 cgtctccaag tccatcagcg gctacggcat gccgatgtcg ctcgccctct tcaagcccga
     4561 gctggacatc tgggagcccg gcgagcacaa cggcaccttc cgcggcaaca accccgcgtt
     4621 cgtgaccgcc gccgcgaccc tggacaccta ctgggccgac ggccagatgg agaagcagac
     4681 cctggcccgc ggcgagcagg tcgagctggc cctgcgcgcc atctgcgagg agcagtccgg
     4741 cacccagttc cgcggccgcg gcctggtgtg ggggctggag ttcgacgaca agccgcgcgc
     4801 caccgcggtc tgccggcgcg ccttcgagct ggggctgctg ctggagacgt ccggccccga
     4861 gagcgaggtc gtcaagctgc tgcccgcgct gacgatctcc cccgatgacc tggacgaggg
     4921 gctgcggacc ctcgcccgcg cggtccgcga gaccgcatga tcctcccgca ccgcagagaa
     4981 gagaaaggca agaactcacc gtgatcgtcc gatccttcaa ggacatcgag gacaccgaga
     5041 ggcatgtgag gtccaagtcc ggcacctggg agagcaagcg catcgtgctc gccaaggagg
     5101 gcgtcggctt ctcgttgcac gagaccatcc tctacgcggg caccgagacg tcgatgtggt
     5161 acgccaacca catcgaggcg gtgctgtgcg tcgagggcga ggccgaactc accaacgacg
     5221 agaccggtga gaagcactgg atcacgcccg gcaccatgta tctgctcgac ggacatgaga
     5281 agcacacgat gcgccccaaa acggatttcc ggtgcgtctg tgtattcaac ccgccgatca
     5341 ccggacggga ggaccatgat gagaacggcg tatacccgct gatcaccgag accgaggagg
     5401 catgaaccga tgaccgcggt cactgacctg tacccgaccc gtggggctgt ggaggtggcg
     5461 acaccgcgcg tggaccccgt ggtgtggtcc gagcccggag ccccagggcc gctgcaaccg
     5521 tccgagctga gcgatttcga tcgcgacggc ttcctggcga tcgatgagct gatcactccg
     5581 gatgaggtgg cggaataccg cgccgaattg gaccggatga tcgccgatcc ggacgtgcgc
     5641 gccgacgaac gctcgatcat cgagccgacg tcgcaggacg tacggtcggt gttcgaggtg
     5701 caccggatca gcgaggtctt cgccaaactg gtgcgcgacc cgcgtgtggt gggccgcgct
     5761 cggcacatcc tcggctcgga cgtctacgtc caccagagcc ggatcaatgt caagccgggc
     5821 tttggcgcct cgggcttcta ctggcactcg gacttcgaga cctggcacgc cgaggacggg
     5881 ctgccgaaca tgcggaccgt gtcggtctcg gtcgcgctca ccgagaactt cgcacacaac
     5941 ggcagcttga tgatcatgcc gggctcgcac cgcaccttcc tgggctgtgc gggcgcgacg
     6001 ccgaaggaca actacaagaa gtcgcttcag atgcaggacg cgggcacccc gtccgacgag
     6061 gcgctcagcg gcttcgccga caagcacggc atcagacagt tcaccggcaa gcccggctcg
     6121 gcggtctggt tcgactgcaa ctgcatgcac gggtccggcg acaacatcac gccgttctcc
     6181 cgcagcaatg tgttcatcgt gttcaacagc gtggagaaca ccgcggtcga gccgttcgcc
     6241 gcgccggtcc cccggccgga cttcatcggt gcgagggact tcacgcccgt acggtaacaa
     6301 ccgccgtgcg ctgatgtgac gtccgtgatc tgagggaagc cggagtgccc cggcttccct
     6361 cagatttttc cccatctgcc ccgcccgcct gcttggcccg tcaggcttgg ccgcggggag
     6421 ccgactcagc cggacagctt ctccagcagc cggtccacat cggccacggt gttgtagagg
     6481 tggaaggagg cgcggaggtt accggcccgc gccgagacat ggacctccgc ctccgccagc
     6541 cggggcgcgg cgtcgcccag gccgggtacg gagacgatgg ccgagccggg ggcggcgatg
     6601 ggccggtggc cgagtccgat gaccccgtcc cggaagcggt cggcgagcgc gcggtcatgg
     6661 gcggcgatgg cctcggtgcc cagctcctcg atcagcgcca gggagtgccg ggccgccaca
     6721 taggagtaga cgctagggct ctcgtcgaag cggcgggcgg agtgggcgag ttcctccacc
     6781 gggccgtagc agctgtccca gggcttctcc cccgcgaccc agcccgcgaa gacgggggtg
     6841 agtccgccga ggtcctccgg cacggtcagg aaggcggcac cgcgcgggca catcagccac
     6901 ttgtaggcca cgcagacggt gtagtcggcc tcggcggcgt tcaccgggaa ccagccggcc
     6961 gcctggctga cgtccaccag ggtgcgggcc ccatgggtcc gggccgcctc ccggatggcg
     7021 tcgagatcgg cgatccgccc gtccgccgac tggaccgcgc tcacggccac cagcgccgta
     7081 ccgggccgta ccgcctccgc gatctcctcc agcgggacga tgcgcagctt gaggtcgccg
     7141 cggacggcga aggggttgac cagggagctg aagtcgccct cggccaccag gacttcggcg
     7201 ccgggcggca gcgaggtggc gatcatcccc gcgtacacgg cgacggagct gcccgccgcg
     7261 acccgccggg ccggtacgcc gaccagccgg gcgtaggcgg cacgggccgc ctcgaccgcc
     7321 tcgaaggcca cgtcgacgat gggctgtccg gtggccgagc cgtcgaggac cgcctgcacg
     7381 gcggctacgg agcgtgcggg gatcagaccg ctggacgcgg tggtgagata cgtcgtgtgt
     7441 ggcgcgaatt cggcgcccgc caggctctcc atgtccaacc actatggaac gcgctcaccc
     7501 cggcgtccat cgcgatttct tgtgggatgc cttcaagcga tccttatgca tccccgctga
     7561 cctgcggcga ttcctccacg tggcccggca ctcgggtgaa cacaccccgc ggatcggccg
     7621 catcaggccg acagcctcag gccccatacg acgtacgcgg ccgcgggcat gccgaaacgg
     7681 ccgccggaca cgcgggtcag gtccgcggat ccggcggccg tgccgtcgtt ccgggcgtca
     7741 gccgcgctgc tcccggacgc cgttgatccg ccgcggcagc tccagcgggt tctcgtcgcg
     7801 cagctccggc ggcagcaggg cggccggggt gtcctggtag gcgaccggcc gcagccagcg
     7861 ctcgatggcg gtgccgccga ccgaggtgga ggtggaggtg gtggccgggt agggcccgcc
     7921 gtggtgctgg gcgggggcga ccgctacacc ggtcggccag ccgttgacca ggacccggcc
     7981 agccagcggg gtgagctcgg cgatcagctc accggcccgg ccctcggtgc cctcggcctc
     8041 ggcggcggag aggtggacgg tggcggtgag gttgcccggg agcctgccga gcaccgcgct
     8101 gatctcctcg tcgctctcgt agcgcgccac gacggtcacc gggccgaagc actcctccag
     8161 cagcgcgtcg tgcgggccgc tttcggccag ccgccccgcc gggacggtca ggaagcccgc
     8221 gctgaccgtg tgctcaccgc cggcgccggg ggtgaccggg gcctccacat ccgccagctc
     8281 ggcgcgctcc tggaccccgg cgacgaagtt gtcccgcatc cggtggtcga gcaggactcc
     8341 ggcctcggtg ttgctgaccg cctcggtgag ggacttcacc aggccgtcgc ccgccgggcc
     8401 ggccggggcc aggacgaagc cgggcttggt gcagaactgg ccgacaccca gcgtcatcga
     8461 gcccgcgagc cccgcgccga tctggtcgcc gcgctcggcc gccgcggcct cggtgaccac
     8521 caccgggttg aggctgccga gctcgccgtg gaaggggatc ggcaccggcc gggaggccgc
     8581 cgcgtcgtgc agggcgcggc cgccgcggac cgatccggtg aaaccggcgg cggcgatcag
     8641 cggatgcctg accagctcga tgcccgcgtc gaagccgtgc accaccgcga tcacctcggc
     8701 gggcaccccg acccgctcgg cggcgcggcg cagcgcggag gcggccacct cggaggtggc
     8761 ggggtggtcg gggtgggcct tgacgatgac cgggcagccc gcggcgaggg cgctcgcggt
     8821 gtcgccgccg ggcacggaga aggcgagcgg gaagttgctg gcggcgtaga cggcgacgac
     8881 gccgagcggc agcttgtagc ggcgcagatc gggccagggc ggggtctggg tggcgtcggc
     8941 gtggtcgatg cggacgtcga ggaagccgcc ctcgtccacg atggtcgcga aggaccgcag
     9001 ctggtaggtg gtgcgggcca gctcgcccgt gagccgggcg gggccgagcg cggtctccgc
     9061 gtcggcggcc tcgaccagca ggtcacgggc ctcgtcgagc aggtcggcgg ccgtgcgcag
     9121 caaggcggcg cgcaccgcgc ggtcggccag ggaaccgcgg gcggcatggg cggcgcgcac
     9181 cacgcggtcg acctcctccg ccgtggcttc gaccgctacc tgctcgcgct gctttccggt
     9241 tcgggggtcg acgctccaca ctggtgctga caccgcggat tcctcctgct gtggtgttac
     9301 cgctgcttgc gtctcacaca tcgacggtgt tcgatatgct gaacgctgtc ccgggtgatg
     9361 aatttccccc tgggactcta tggctggtcg aacagagggg tcaaggcgat gtcagctgcc
     9421 gagacgggtg gggcccaggt caagtcggcg gtccgcaccg tcgaactgct ggagttcttc
     9481 gccgggcggc ccggtatgca cagcctcgcc gcggtgcagg aggccgtggg ctatcccaag
     9541 tcgagcctct acatgctgct gcgcaccctg gtggagctcg gctgggtgga gaccgacgcc
     9601 accggcaccc gctacggcat cggggtgcgc gccctgctcg tcggcacctc ctacatcgac
     9661 ggcgacgagg tcgtggccgc ggcccgcccc accctggacc ggctctcgga cgacaccacc
     9721 gagaccatcc acctcgcccg gctcgacggc accaatatcg tctatctcgc cacccggcag
     9781 tcgcagcact atctgcgccc cttcacccgc gtcgggcgcc ggctgcccgc ccactccacc
     9841 tcgctgggca aggcgctgct ggccacgtac tccgacgagc aggtgcgcaa gctgctgccg
     9901 gagacgctga gccagctcac cgagcacacc atcaccgacc gcgagaagct gatcgaggag
     9961 ctgcacctcg tccgggagca ggggtacgcg gtggaccgcg aggagaacac cctgggcctg
    10021 cgctgcttcg gcatcgccat cccgtaccgc accccctcgc gcgacgccat cagctgctcg
    10081 gtgccggtgg cccggctcac ccccgcccat gagcagatga tcaaggatgc cctgttcgac
    10141 gcccgggacc ggctcaccct cgcgacgcgc cgcctctgac ccacgggagg acggccgcct
    10201 ccgccatgcg gcggaggggg atcgtcgggc ggggtgaggc gccgcgccca ccgcgccggg
    10261 aggctggtga ccatgaccca caccctcccc ggcccccgga cgcgctccgc acccgcccgc
    10321 ctgcccggtg acctgaccgc gcggatcctg cgggccgtcg cgatcctcgc ctgtctcccc
    10381 tacatcggcc tcaaggccgc ctgga
//
