LOCUS       NZ_LJSN01000003        11297 bp    DNA     linear   CON 29-MAR-2024
DEFINITION  Streptomyces noursei strain JCM 4701 scaffold3, whole genome shotgun
            sequence.
ACCESSION   NZ_LJSN01000003
VERSION     NZ_LJSN01000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces noursei
  ORGANISM  Streptomyces noursei
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 3830602)
  AUTHORS   Graham,D.E., Mahan,K.M., Klingeman,D.M., Fida,T., Giannone,R.J.,
            Hettich,R.L., Parry,R.J. and Spain,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-SEP-2015) Biosciences Division, Oak Ridge National
            Laboratory, PO Box 2008, MS-6038, Oak Ridge, TN 37831-6038, USA
REFERENCE   2  (bases 1 to 3830602)
  AUTHORS   Graham,D.E., Mahan,K.M., Klingeman,D.M., Fida,T., Giannone,R.J.,
            Hettich,R.L., Parry,R.J. and Spain,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-SEP-2015) Biosciences Division, Oak Ridge National
            Laboratory, PO Box 2008, MS-6038, Oak Ridge, TN 37831-6038, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2015
            Assembly Method :: SPAdes v. 3.6; SMRT Analysis v. 2.3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300.0x
            Sequencing Technology             :: Illumina MiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002891315.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 20:38:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,947
            CDSs (total)                      :: 8,851
            Genes (coding)                    :: 8,489
            CDSs (with protein)               :: 8,489
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 362
            CDSs (without protein)            :: 362
            Pseudo Genes (ambiguous residues) :: 0 of 362
            Pseudo Genes (frameshifted)       :: 118 of 362
            Pseudo Genes (incomplete)         :: 301 of 362
            Pseudo Genes (internal stop)      :: 23 of 362
            Pseudo Genes (multiple problems)  :: 74 of 362
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:29:39
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2341903
            Orig. end                         :: 2353200
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LJSN01000003.1.
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: http://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11297
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="15"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11297
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11297
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6297](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6297
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(215..1114)
                     /locus_tag="AOB60_RS31925"
                     /old_locus_tag="AOB60_30820"
     CDS             complement(215..1114)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088893.1"
                     /locus_tag="AOB60_RS31925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOB60_30820"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_102925810.1"
                     /transl_table=11
                     /translation="MRLDGVGRRYGLRGPWVLREVRLDVPAGALLRIEGTNGSGKSTLL
                     RLLAGIDRPTTGRITGRPRTAYVPERFPAALPFTAADYLAHLGRVHGLRTPEAADRAAE
                     WLARFGAAGHARTPLAELSKGTSQKVAVAQALLAEPELLVLDEAWTGLDPAARDTLDRA
                     VAERVAAGGTVVFVDHDPRRLAGAASARYRVADGRLLPREPGTAAEPSDGVDAGAGPRT
                     VVEAEGPPGSPPPPGLPGAPDLAPGPGNSLRLTVATVHSDALLRALLTARPPWHIRAVA
                     AAGPPGPGTPAPGEAPRP"
     gene            1337..2047
                     /locus_tag="AOB60_RS31930"
                     /old_locus_tag="AOB60_30825"
     CDS             1337..2047
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049034.1"
                     /locus_tag="AOB60_RS31930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOB60_30825"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_102926415.1"
                     /transl_table=11
                     /translation="MDDQRAQAPTEPAPEYVLPLVVRVERAAPPGRTDALETAARAVLV
                     LLSDERATDAGEWARAVHDWQDSRIRKVVRRARGAEWRRAEALPGITVTGREAEVRVFP
                     PVPLDGWPKDLARLQVSGTELDEPQAPAEPADGAPVLWLNPGLKMSAGKAMAQAGHGAQ
                     LAWWELSTAEREAWRAAGFPLAVRTATPAAWPALTTSGLPVVRDAGFTEIAPGKTVVTE
                     GGSRFCPLPCTRRP"
     gene            complement(2457..3056)
                     /locus_tag="AOB60_RS31940"
                     /old_locus_tag="AOB60_30835"
     CDS             complement(2457..3056)
                     /NRPS_PKS="Domain: Polyketide_cyc2 (41-191). E-value:
                     5.5e-17. Score: 54.4. Matches aSDomain:
                     nrpspksdomains_AOB60_RS31940_Polyketide_cyc2.1"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008741218.1"
                     /locus_tag="AOB60_RS31940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOB60_30835"
                     /product="SRPBCC family protein"
                     /protein_id="WP_258018339.1"
                     /transl_table=11
                     /translation="MFAVPIVIGLLGAVAPPAGAAPHRAGTSLTCRGAGVDPRARVRYR
                     TEAVIHAPLRTIWKLQTDVVRWPSWQAGVRTMERLDHGPFRKGSAFRWTTPVPPNPTTP
                     ATELEITSTVRQLKRNSCIRWTGPAVGARLRIDGVHVWNFAEVEGGVRVSTEETHAGAQ
                     VDANVPAATAILRQGLEAWLRELKVTAEATTLDQRR"
     aSDomain        complement(2484..2933)
                     /aSDomain="Polyketide_cyc"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AOB60_RS31940_Polyketide_cyc2.1"
                     /domain_subtypes="Polyketide_cyc2"
                     /evalue="5.50E-17"
                     /label="AOB60_RS31940_Polyketide_cyc2.1"
                     /locus_tag="AOB60_RS31940"
                     /protein_end="191"
                     /protein_start="41"
                     /score="54.4"
                     /tool="antismash"
                     /translation="VRYRTEAVIHAPLRTIWKLQTDVVRWPSWQAGVRTMERLDHGPFR
                     KGSAFRWTTPVPPNPTTPATELEITSTVRQLKRNSCIRWTGPAVGARLRIDGVHVWNFA
                     EVEGGVRVSTEETHAGAQVDANVPAATAILRQGLEAWLRELKVTAE"
     misc_feature    3329..3345
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 14.79"
                     /tool="antismash"
     misc_feature    complement(3339..3352)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.22"
                     /tool="antismash"
     misc_feature    3340..3353
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 12.43"
                     /tool="antismash"
     misc_feature    complement(3375..3391)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 17.71"
                     /tool="antismash"
     gene            3435..4088
                     /locus_tag="AOB60_RS31945"
                     /old_locus_tag="AOB60_30840"
     CDS             3435..4088
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006350473.1"
                     /locus_tag="AOB60_RS31945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOB60_30840"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_102925813.1"
                     /transl_table=11
                     /translation="MNGTDPKTPRERYRAQVRAEIKERAWEQIATAGVPALSLNAVAKQ
                     MGMSGPALYRYFANRDELITALVKDAYQSLAEALRAASVGGADLAVLAHALRGWALADP
                     QRYFLVYGTPVPGYHAPEDITGIADQIMAVLLDACAALAADEPTTPFSAHLEAHREWAH
                     GHPAPPAALHKALAFWSRLHGVLSLELTGQFNGMGLDPALFFAAELDGLLAPGA"
     gene            4194..4844
                     /locus_tag="AOB60_RS31950"
                     /old_locus_tag="AOB60_30845"
     CDS             4194..4844
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1116:
                     Homoserine/threonine efflux protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016578488.1"
                     /locus_tag="AOB60_RS31950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOB60_30845"
                     /product="LysE family translocator"
                     /protein_id="WP_073448893.1"
                     /transl_table=11
                     /translation="MLTSLIGFTVVAALLTVSPGPDFAVVLRTALGSGRRAALCSALGI
                     AAGCFVWGLAGAVGLTAMLAASQTAYDALRVAGAVYLVWLGVQALRGARRRSGADAAED
                     GPAEEAAPTVPGSPWRAFRGGLLTNVLNPKVGVVYLSLLPQFIPHGAPVVGTTLLLVAV
                     HAVLGVLWLGGVALAVHRARAVLQRPRVRRRLDQATGGVLVALGAAVGWEVAR"
     gene            5001..6297
                     /locus_tag="AOB60_RS31955"
                     /pseudo=""
     CDS             5001..6297
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944755.1"
                     /locus_tag="AOB60_RS31955"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="DUF692 domain-containing protein"
                     /pseudo=""
                     /sec_met_domain="DUF692 (E-value: 7.6e-95, bitscore: 314.7,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRRLGTGIGWRPEIAAEIAALPGVDWVEVVAENVCPGHLPDALRE
                     LRERGTTVIPHGVSLGLGGADRPDAGRLAALAERATALEAPLLTEHIAFVRAGGPLTAS
                     PALEAGHLLPMPRTQDALDVLCENVRIAQDALPVPLALENTAALFAWPGEELTEGQFLS
                     ELVERTGVGLLIDVANLHTNHVNRGEDPTAALDELPVEALVYVHVAGGVERDGVWHDSH
                     AHPVTPPVLDVLAELCARTDPPGVLLERDDDFPEGGELAGELDAIRAVVEEARRVRAA"
     gene            6307..7296
                     /locus_tag="AOB60_RS31960"
                     /old_locus_tag="AOB60_30860"
     CDS             6307..7296
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="AOB60_RS31960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOB60_30860"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_102925814.1"
                     /transl_table=11
                     /translation="MSFTQGVYLGLGLSSLALLVGALVVRLRTPAVGRAAGPTRDVREL
                     AFLAGGPGRVADTALWVMHKEGRLAIGGPGVVTVRRAAAHDPVETAVLDAVAATPGGAL
                     AAVRGRVMRSAAVQEVGDRLAARGLLRPPRAGRGWRLLAGLQVKASVVLFAVALVLSFL
                     AVRDGGGPWAPPVAVRVVPAVAVGLLVGLPCRRLFTRRITRAGRSVLAAARPRGTRPDD
                     LRGLEAAWRVAGLAVALGGTAVLANELLRDHFVQAQGAVAASTPSGGSSAGSGGCGGDA
                     GSAWCGSSDGGGDSGCGASSCGGGSSCGGSSGCGGSGCGGGGCGGGGD"
     gene            7434..8222
                     /locus_tag="AOB60_RS31965"
                     /old_locus_tag="AOB60_30865"
     CDS             7434..8222
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536945.1"
                     /locus_tag="AOB60_RS31965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOB60_30865"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_073448892.1"
                     /transl_table=11
                     /translation="MWLLFLLLAWVAAVVSCVRVCRTAVVTGQAQHRAQDTPPDTAPDA
                     DGVHAGLTLYETAYLSGGPHRLADVVLVLMAQQRRLHLAHTGWATVVDPVGQDPVERAA
                     LTAIGPEGQCRIPAVRTALSGTDAVRGLADRLVDAGLAAPAAIRTAVRQVRGAALVVLL
                     AAGATYWTAPSGEADRLVLAWFALPLILALGTWAIARAEARPSTGWATEAGSARLRQEH
                     PGVRDRRPAPEPAGLTGLALHGPAVLVDPGLRAALHSSGA"
     gene            8359..10068
                     /locus_tag="AOB60_RS31970"
                     /old_locus_tag="AOB60_30870"
     CDS             8359..10068
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (91-511). E-value:
                     1.2e-17. Score: 56.3. Matches aSDomain:
                     nrpspksdomains_AOB60_RS31970_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536946.1"
                     /locus_tag="AOB60_RS31970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOB60_30870"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_258018197.1"
                     /sec_met_domain="PF00561 (E-value: 2.3e-21, bitscore: 74.2,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRALALYGALGSLVLSGLASAPAGGAARDPAAPPAPVHPLTFGRC
                     AGVEHLPASVECGTLTVPLDYARPHGKQIKLTVSRTRATSSAERQGALVFNPGGPGASS
                     MQFPLYGALPTWHRTARAYDFVGYAPRGVGRSAPLSCQDPKDFTKAPTDSPRYPSESFK
                     QKKVAEAKAYAHGCAQRAGADLPFYNSVNNARDLDMLRAALGEKKLTFMGASYGTYFGA
                     VYATLFPGHVRRMVFDSVVNPDPRQIWYQNNLDQSVAFERRWGDWLRWVAKHDSTYHLG
                     SDKQAVQHSYDTVIQRLRHAPVNGKIGPGQLQAAYLKTGYNDAFWPMRAEALSKYLRGD
                     AQLLIAQALPSGDGQGEENANAVYTAVECNDSAWPRNWHVWDRDNTALARTAPFETWDN
                     AWMNLPCAFWPERRQSAADGVEEAIRTTTQRAVKGASAGDALAHASQQAADTDLDDALY
                     GRGRPLDIRTEPGALPPVLLLAAERDAATPYPGALELQRRLPGSSLVTERDAGTHGIAY
                     GDNLCVNGYVETYLLRGKVPGHRSYCGPRAEPVPGQPLQRARPTLVQQVKGR"
     aSDomain        8632..9891
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AOB60_RS31970_Abhydrolase_1.1"
                     /evalue="1.20E-17"
                     /label="AOB60_RS31970_Abhydrolase_1.1"
                     /locus_tag="AOB60_RS31970"
                     /protein_end="511"
                     /protein_start="91"
                     /score="56.3"
                     /tool="antismash"
                     /translation="ALVFNPGGPGASSMQFPLYGALPTWHRTARAYDFVGYAPRGVGRS
                     APLSCQDPKDFTKAPTDSPRYPSESFKQKKVAEAKAYAHGCAQRAGADLPFYNSVNNAR
                     DLDMLRAALGEKKLTFMGASYGTYFGAVYATLFPGHVRRMVFDSVVNPDPRQIWYQNNL
                     DQSVAFERRWGDWLRWVAKHDSTYHLGSDKQAVQHSYDTVIQRLRHAPVNGKIGPGQLQ
                     AAYLKTGYNDAFWPMRAEALSKYLRGDAQLLIAQALPSGDGQGEENANAVYTAVECNDS
                     AWPRNWHVWDRDNTALARTAPFETWDNAWMNLPCAFWPERRQSAADGVEEAIRTTTQRA
                     VKGASAGDALAHASQQAADTDLDDALYGRGRPLDIRTEPGALPPVLLLAAERDAATPYP
                     GALELQRRLPGSSLVTERDAG"
     gene            complement(10145..10882)
                     /gene="hemQ"
                     /locus_tag="AOB60_RS31975"
                     /old_locus_tag="AOB60_30875"
     CDS             complement(10145..10882)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536947.1"
                     /locus_tag="AOB60_RS31975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOB60_30875"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_073448890.1"
                     /transl_table=11
                     /translation="MDDMTDASTPASDKIPNAGKKAKDLNEVIRYTLWSVFKLRDVLPE
                     DRSGYADEVEELFAQLAAKDVVVRGTYDLSGLRADADVMIWWHSENSDDLQEAYNRFRR
                     TRLGRALEPVWSNMALHRPAEFNKSHIPAFLADEHARDYVSVYPFVRSYDWYLLPDEDR
                     RRMLADHGKMARGFPDVRANTVASFSLGDYEWILAFEADELHRIVDLMRHLRGSEARQH
                     VREEIPFYTGRRKPVAELVAGLA"
ORIGIN
        1 gcggccggcg gctcgttggt ggcacagatc cgggccagcc agacggagac cggcagcagc
       61 ccgccggcgg cgtagccgta cgcgtccagg atcgggccgc cggtctggac gccgatcgcg
      121 aggaacgccg cgtaggcgag gagcggcggc agccagcggt aggaaccgac gagcagtgcc
      181 gcctgatagc ggatcagcgc gggcagggag gcggtcatgg acggggagcc tctccgggag
      241 caggggttcc ggggccgggc ggcccggcgg cggcgaccgc ccggatgtgc cagggcgggc
      301 gcgcggtgag cagggcgcgc agcagggcgt cggagtggac ggtggcgacg gtcagccgca
      361 ggctgttgcc ggggccgggg gcgaggtccg gcgcgcccgg caggccgggc ggcggcgggc
      421 tgcccggcgg accctccgcc tcgacgacgg tccgcgggcc ggcaccggcg tcgaccccgt
      481 cggacggctc ggcggcggtg ccgggctccc ggggcagcag ccggccgtcg gcgacgcggt
      541 agcgggcgct cgcggccccg gccagtcggc gcgggtcgtg gtcgacgaag accacggtgc
      601 cgccggcggc gacccgctcg gcgaccgcgc ggtcgagggt gtcgcgggcg gccgggtcca
      661 gcccggtcca cgcctcgtcc aggacgagga gttcgggctc ggcgagcagt gcctgggcga
      721 ccgcgacctt ctggctcgtc cccttggaca gctcggccag cggggtccgg gcgtgaccgg
      781 cggcgccgaa gcgcgccagc cactcggcgg cacggtccgc cgcctccggt gtccgcaggc
      841 cgtggacgcg gccgaggtgg gcgaggtaat cggcggcggt gaacggcagg gcggccggga
      901 accgctcggg cacgtaggcg gtgcgcggac gcccggtgat ccgacccgtg gtgggccggt
      961 cgatgccggc gagcagccgc agcagcgtgg acttgccgct gccgttggtc ccctcgatgc
     1021 gcaacagcgc acccgccggc acatcgagcc gcacctcgcg cagcacccag ggcccgcgca
     1081 ggccgtagcg ccgcccgacc ccgtccagcc gcatccgctg cgcttcctgc cgcggcatcg
     1141 gccgcacctc ccccgttccc ctgttcccct gtagccgccg acggtagccg ggcgcgggca
     1201 gcaccgcgtc ggcgggcgcg ggttcggcga ccgtcgaccg gcgcgaggag ggactgtcgg
     1261 tggcggctgg cagactgatc gggtgaccag taccgagacc gccggcccgt ccggcgagca
     1321 cagcgcgccc acccggatcg acgaccagcg ggcgcaggct cccacggagc cggcgccgga
     1381 gtacgtcctg ccgctggtgg tgcgtgtgga gcgggccgca ccccccgggc gcacggacgc
     1441 gctggagacc gcggcgcggg cggtgctggt gctgctctcc gacgagcggg ccaccgacgc
     1501 gggcgagtgg gcccgtgcgg tgcacgactg gcaggactcc cggatacgga aggtggttcg
     1561 gcgggcgcgc ggagcggagt ggcggcgggc cgaggcgctg cccggcatca cggtcaccgg
     1621 ccgcgaggcc gaggtgcggg tcttcccgcc ggtgccgctg gacggctggc cgaaggacct
     1681 cgcccggctc caggtctccg gcaccgagct ggacgaaccg caggcccccg cggagcccgc
     1741 ggacggcgcg ccggtcctgt ggctcaatcc cgggctgaag atgtcggcgg gcaaggcgat
     1801 ggcacaggcc gggcacggcg cacagctggc ctggtgggag ctgtccacgg cggagcggga
     1861 agcctggcgc gcggcagggt tcccgctggc cgtgcgcacc gcgacgcccg cggcctggcc
     1921 ggcactgacg accagcggac tgcccgtggt gcgggacgcc gggttcaccg agatcgcgcc
     1981 cgggaaaacc gtggtgaccg aagggggcag ccgattctgc cccctcccct gcacacgacg
     2041 gccgtaggtc gtgcggattg cggaccacgg gctgcccggc gttccggacg gcgcccgacc
     2101 gaagtcggcc ctcggggcga caccgtggcc acaccgggca gcaccgggca gcacagcagg
     2161 tctcgcctga cgcactgtca gcaactacgg tcggaagatc tccacgcgag gcgcggcggc
     2221 aggggtggcg gcctcgccgg cggtagcacc gacgcaggca caggccccgg cgccgaacgt
     2281 gccgccgggg atgcagggga cgcaggggac cagatcgaag gtccgccgcc tctatcggac
     2341 gcccctctcc accaccctgc gcacccgcgc catccgcgtc atccgcgtca tccgccgcga
     2401 actggccgat ttcgacggtg agttccgcgc ccgtcgcgtc atcgggacgt cggggttcag
     2461 cgtcgctgat cgagggtggt cgcttcggcg gtgaccttca gctcgcgcaa ccatgcttcg
     2521 aggccctgac ggaggatcgc ggtcgcggcg ggaacgttcg cgtcgacctg ggcgccggcg
     2581 tgggtctcct cggtgctcac ccgaaccccg ccctcgacct cggcgaagtt ccatacgtgg
     2641 acgccgtcga tgcgcagcct cgcgccgacc gcggggccgg tccagcggat gcaggagttg
     2701 cgcttgagct gccggacggt cgaggtgatc tccaactcgg tggcgggagt cgtggggttg
     2761 ggcggtaccg gggtcgtcca gcggaacgcc gaacccttcc ggaaggggcc gtgatcgagg
     2821 cgttccatgg tcctaacgcc ggcttgccag gacggccagc gcaccacgtc ggtctgcagc
     2881 ttccagatgg tgcgcaacgg cgcgtgaatc acggcctcgg tccgatagcg aacccgcgcg
     2941 cgggggtcga cgccggcccc gcggcaggtg agggaggtgc cggcgcggtg cggggcggcc
     3001 ccggcgggcg gggcgacggc accgaggagg ccgatgacga tcggcacggc gaacaggacg
     3061 gcgcgggtgc gggtgcgggc gcgagtgcga gcgtggatgt cggtgccgac cccagcgctt
     3121 gcactgtggc tgcggctgtg gctgtggctg tggctgtggc tgacgatgcc ggacatgggg
     3181 ttccccttgt cgattggtgg gctcttgcct gctgttgggc tgtcgtctcg tcggttgccg
     3241 aggcccagcc ggattccgtg agggcgttga ctgttgagct cgcagcggtg cgcgacgccc
     3301 ctgcacgagg cgttagatac tcgcccgttc gttagaagtt ataactatcc gagagcgaag
     3361 ggtcaagaga ttgcgtgata ggttctaacg aaagcttgag gtcataggca aacgggcggg
     3421 gaaggcggca ggtcatgaac gggacggacc cgaagacccc tcgggagcgc taccgcgccc
     3481 aggtgcgcgc ggagatcaag gaacgcgcct gggagcagat cgccacagcg ggggtgcccg
     3541 cgctctccct caacgcggtc gccaaacaga tgggcatgag cggccccgcg ctctaccgct
     3601 acttcgccaa ccgcgacgag ctgatcaccg cactcgtcaa ggacgcctac cagagcctcg
     3661 ccgaggccct ccgcgccgcc tcggtcggcg gtgccgacct ggccgtgctg gcgcacgccc
     3721 tgcgcggctg ggccctggca gacccgcagc ggtacttcct cgtctacggc acccccgtgc
     3781 ccggttacca cgcccccgag gacatcaccg gcatcgcgga ccagatcatg gccgtcctgc
     3841 tcgacgcctg cgcagccctc gccgcggacg aaccgacaac gccgttcagc gcccacctgg
     3901 aagcacaccg ggaatgggcc cacggccatc ccgccccgcc ggcggctctc cacaaagccc
     3961 tggccttctg gtcccggttg cacggtgtcc tgtccctgga gctcaccggc cagttcaacg
     4021 gcatgggcct ggaccccgcc ctgttcttcg cggccgaact ggacggcctg ttggcccccg
     4081 gagcgtgacg tcgtgcgcgg ggcgacggcc gggtcgccgg cggcggcccc cgttggaccg
     4141 ctcctgcgtc cccgccggcg gcccggccca cggcgggaca taccgtgtca cccatgctga
     4201 cctcgctcat cggcttcacc gtggtggccg cactgctcac cgtctccccc ggcccggact
     4261 tcgcggtggt tctgcggacc gcgctcggct cggggcggcg cgccgcgctg tgcagtgcgc
     4321 tggggatagc cgccggctgc ttcgtgtggg gactggccgg ggcggtgggg ctgaccgcga
     4381 tgctggccgc ctcgcagacg gcgtacgacg cgctgcgggt ggccggcgcg gtgtatctgg
     4441 tgtggctcgg ggtgcaggcg ctgcgcggcg cccggcgtcg gagcggcgcg gacgcggcgg
     4501 aggacgggcc ggcggaggag gccgccccga ccgttccggg cagcccctgg cgggcgttcc
     4561 gcggcgggct gctgaccaac gtcctcaacc ccaaggtggg agtggtctac ctctccctgt
     4621 tgccgcagtt catcccgcac ggcgcgccgg tggtcggtac gacgctgctg ctggtggccg
     4681 tgcacgcggt gctgggtgtg ctgtggctgg gcggggtggc cctggcggtg caccgggcgc
     4741 gggcggtgct gcagcgcccg cgggtgcggc ggcggctgga ccaggccacc ggcggggtgc
     4801 tggtggcgct gggcgcggcg gtcgggtggg aggtcgcccg ctgagccgcg gcgggacgca
     4861 acggccggcg cccggcacgg tccgggccgg cctctggtga gagaagctca aatcaacctc
     4921 tgaaccgcgc gggtgcggga ttcgccccgc ccgcgccggg tcaggactac cccgaccgtg
     4981 gggagcacaa agggggaacc atgcgtcgtt tggggacggg catcggctgg cggccggaga
     5041 tcgccgcgga gatcgccgcg ctgcccgggg tggactgggt ggaggtggtg gccgagaacg
     5101 tctgccccgg ccatctcccg gacgccctgc gggagttgcg ggaacgcggc acgacggtga
     5161 tcccgcacgg ggtctcgctc gggctcggcg gcgccgaccg gccggacgcc gggcggctgg
     5221 cggccctcgc cgagcgggcc accgcgctgg aggccccgct gctcaccgag cacatcgcgt
     5281 tcgtccgcgc cggcggcccg ctgaccgcgt cgccggcgct ggaggccggg cacctgctgc
     5341 cgatgccgcg cacccaggac gcgctggacg tgctgtgcga gaacgtccgg atcgcccagg
     5401 acgcgctgcc ggtgccgctg gccctggaga acaccgccgc gctgttcgcc tggcccggcg
     5461 aggagctgac cgaggggcag ttcctgtccg agctggtcga gcgcaccggg gtcgggctgc
     5521 tgatcgacgt cgccaacctc cacaccaacc acgtcaaccg cggcgaggac ccgaccgcgg
     5581 ccctcgacga gctgccggtc gaggcgctcg tctacgtcca tgtcgcgggc ggcgtcgagc
     5641 gggacggggt gtggcacgac agccacgccc acccggtgac cccgccggtc ctggacgtcc
     5701 tcgcggagct gtgcgcccgg accgatccgc cgggcgtgct gctggagcgc gacgacgact
     5761 tccccgaggg cggcgagctg gccggtgaac tcgacgcgat ccgcgccgtg gtggaggagg
     5821 cgcgccgtgt ccgggcggct tgaggcggtg gcggacaccg gtccggcccg tgagcgattg
     5881 gcgctggcgc aggccgcgct gctgtccgca ctggtggccg ggacgcccgc cccggagggc
     5941 ttcgacccgg cccggctgcg ggtgcagagc cgggccctgg ccgccaagcg ggcctcggtg
     6001 gtcgccaagg tggcaccgga gctgacggag gtcctcggcg acggctatcg gtcggccttc
     6061 ctcggctacg cccagcgccg tcccatggcc ggcggctacc gccaggacgc cctggacttc
     6121 gcggcgttcc tgctggcgca ggaccgcccc gcggacccag tggcacgacg gcggctgacg
     6181 cactggtggc gcgaccgcgc cggtccgcga ccgccgtccg ggcgccgcgc ggcgcagctc
     6241 gcgcgggccg ccgcccggcg ggcaggaacc ctgcagcgcg cgctgcgtcg gaagtgaggt
     6301 gccgtcgtgt cgttcacgca aggggtgtat ctgggcctcg gcctgtcgtc gctggcgctg
     6361 ctcgtcgggg ccctggtggt gcggctgcgg acgccggcgg tcgggcgggc ggccggaccg
     6421 acccgtgacg tccgggagtt ggcgttcctg gccggcgggc cgggccgggt ggcggacacc
     6481 gcgctctggg tgatgcacaa ggaggggcgg ctggcgatcg gcggcccggg cgtggtgacg
     6541 gtgcggcgcg ccgccgcgca cgacccggtg gagacggccg tgctggacgc ggtcgccgcc
     6601 accccgggcg gggcgctggc cgccgtccgc gggcgggtga tgcgcagtgc ggcggtgcag
     6661 gaggtcggcg accggctggc ggcccggggg ctgctgcgcc cgccgcgcgc cggccggggc
     6721 tggcggctgc tggccgggct ccaggtgaag gcgagcgtcg tgctcttcgc cgtcgcgctg
     6781 gtgctgtcct tcctcgccgt ccgcgacggc ggtggtccgt gggcgccgcc ggtggccgtc
     6841 cgggtcgtcc cggcggtggc cgtgggcctg ctggtcgggc tgccgtgccg gcggttgttc
     6901 acccgccgga tcacccgggc gggccggtcc gtcctggccg cggcccggcc ccggggcacc
     6961 cgtcccgacg acctccgggg cctggaggcg gcctggcggg tcgccgggct ggcggtggcg
     7021 ctggggggca ccgcggtgct cgcgaacgag ttgctgcggg accacttcgt gcaggcccag
     7081 ggggcggtgg cggcctcgac accgtccggc gggtcgtcgg cgggctccgg cgggtgcggc
     7141 ggggacgcgg gctcggcgtg gtgcggctcc tcggacggcg gtggggactc cggatgcggg
     7201 gcgtcctcgt gcggcggcgg gtcgtcgtgc ggcgggagtt cgggctgtgg gggctccggg
     7261 tgcggcggcg ggggctgcgg cggcggcggt gactgacgaa ctgtcggatc aggtgtccgc
     7321 acgaaggggc tggtcggggc ggcgagttcg gcgccccggc cggccggggc gcactatcca
     7381 cagggtgaga aacgtcatgg cggccgcgcc cgccgtcccg tagaacgtcc gctatgtggc
     7441 tcctcttcct cctgctcgcc tgggtggcgg cggtcgtctc ctgtgtccgg gtctgccgca
     7501 cggcggtcgt caccggccag gcccagcacc gcgcccagga caccccgccg gacaccgcgc
     7561 cggacgccga cggcgtccac gccgggctga ccctgtacga gacggcctat ctctccggcg
     7621 gcccgcaccg actcgccgat gtggtgctcg tcctcatggc ccaacagcgg cggctgcacc
     7681 tcgcccacac tggctgggcc acagtcgtcg acccggtcgg ccaggacccc gtcgagcggg
     7741 ccgcgctcac cgccatcggc cccgaggggc agtgccgcat acccgccgtc cggaccgccc
     7801 tgtccggcac cgatgcggtc cgggggctgg ccgaccggct ggtcgacgcc ggtctcgcgg
     7861 cgcccgccgc gatccgcacc gcggtccgcc aggtccgcgg cgccgccctc gtggtgctgt
     7921 tggccgccgg cgcgacctac tggacggccc cgtccggcga ggccgaccgg ctggtgctgg
     7981 cgtggttcgc gctcccgctg atcctcgccc tcggcacctg ggccatcgcc cgcgcggagg
     8041 cccgtccgtc caccggctgg gccaccgagg ccggctcggc acggctccgg caggagcacc
     8101 ccggcgtgcg ggacagacgt ccggcgccgg aaccggccgg cctgacggga ctcgccctgc
     8161 acggacccgc cgtgctcgtc gatcccggtt tgcgcgcggc gctccactct tccggggcat
     8221 agcgtccgac accgccgtac ggtgctttac ttcgccctcg gttggcccaa ctatcccttt
     8281 tgtatgccca ccgtgcgacc ccgtgcagcg agcccctccc cggagccccg caccgcgcac
     8341 catacgaagg gacggctcgt gagagcactt gcgttgtacg gcgccctcgg gtcgctggtc
     8401 ctgagcggac tcgcctccgc accggccggc ggcgccgccc gggacccggc ggcgccgccc
     8461 gcccccgttc accccctcac cttcggccgg tgcgcgggcg tggagcacct cccggcctcc
     8521 gtcgagtgcg gcacgctcac cgtcccgctc gactacgccc ggccgcacgg caagcagatc
     8581 aagctgacgg tcagccggac ccgggccacc tcgtccgccg aacggcaggg cgccctggtc
     8641 ttcaaccccg gcggccccgg cgcgtccagc atgcagttcc ccctctacgg agcgctgccg
     8701 acctggcacc ggaccgcccg cgcctacgac ttcgtcggct atgcgccgcg cggggtcgga
     8761 cgcagcgccc cgctgtcctg ccaggacccc aaggacttca ccaaggcgcc cacggacagc
     8821 ccgcggtacc ccagcgagtc cttcaagcag aagaaggtgg cggaggcgaa ggcgtacgcc
     8881 cacggctgcg cccaacgggc cggcgccgac ctgccgttct acaactcggt caacaacgcc
     8941 cgggacctgg acatgctgcg ggcggcgctc ggcgagaaga agctgacgtt catgggcgcg
     9001 tcctacggga cgtacttcgg ggcggtctac gcgacgctgt tccccggcca tgtccgccgg
     9061 atggtcttcg acagcgtggt caaccccgat ccccggcaga tctggtacca gaacaacctc
     9121 gaccagagcg tcgccttcga gcgccgctgg ggcgactggc tgcgctgggt cgccaagcac
     9181 gactccacct accacctggg atccgacaag caggcggtgc agcactccta cgacaccgtc
     9241 atccagcggc tgcggcacgc gccggtcaac gggaagatcg gccccgggca gctccaggcg
     9301 gcgtacctca agaccggcta caacgacgcg ttctggccga tgcgcgccga ggcgctgtcg
     9361 aagtacctgc gcggcgacgc ccagctgttg atcgcccagg cactgccctc cggggacggc
     9421 cagggcgagg agaacgccaa cgccgtctac accgccgtcg agtgcaacga ctcggcctgg
     9481 ccgcgcaact ggcacgtctg ggaccgggac aacaccgccc tggcccgcac cgcgccgttc
     9541 gagacctggg acaacgcctg gatgaacctg ccgtgcgcct tctggccgga gcgccggcag
     9601 tcggcggcgg acggcgtcga ggaggcgatc cgcaccacca cccagcgggc cgtcaagggc
     9661 gcgtcggccg gggacgccct ggcccacgcc tcccagcagg ccgccgacac cgacctggac
     9721 gacgcgctgt acgggcgggg ccgcccgctg gacatccgta ccgagcccgg cgcgctgccg
     9781 ccggtgctgc tgctggccgc cgaacgggac gcggccaccc cctacccggg cgcgctggag
     9841 ctgcagcggc ggctgcccgg ctcgtccctg gtgaccgagc gggacgcggg cacccacggc
     9901 atcgcgtacg gcgacaacct ctgtgtgaac ggctacgtcg agacgtacct gctgcgcggg
     9961 aaggtccccg ggcaccggtc gtactgcgga ccgcgcgccg agccggtgcc ggggcagccg
    10021 ctccagcggg cccggccgac gctggtgcag caggtgaagg ggcggtagcc gccccggagc
    10081 gtggaggtcg gggccgtccc ttcgggggcg gccccgacgt ccgtgttccg gggtgtccga
    10141 ccggtcaggc caggccggcg accagctcgg cgaccggctt gcggcggccg gtgtagaacg
    10201 ggatctcctc gcggacgtgc tggcgcgcct cggagccgcg caggtgacgc atcaggtcga
    10261 cgatgcggtg gagctcgtcg gcctcgaagg cgaggatcca ctcgtagtcg ccgagcgaga
    10321 aggaggcgac ggtgttggcg cggacgtcgg ggaagccgcg ggccatcttg ccgtggtcgg
    10381 cgagcatccg gcggcggtcc tcgtcgggga ggaggtacca gtcgtaggag cgcacgaacg
    10441 ggtagacgct cacgtagtcg cgggcgtgct cgtcggccag gaaggccggg atgtgcgact
    10501 tgttgaactc ggcgggacgg tgcagcgcca tgttcgacca gaccggctcc agcgcgcggc
    10561 ccagacgggt gcggcggaag cggttgtacg cctcctggag gtcgtcggag ttctccgagt
    10621 gccaccagat catcacgtcg gcgtcggcgc gcagcccgga gaggtcgtag gtgccgcgga
    10681 ccaccacgtc cttggcggcg agctgggcga acagctcctc gacctcgtcg gcgtagccgg
    10741 accggtcctc ggggaggacg tcgcgcagct tgaagaccga ccagagggtg tagcggatga
    10801 cctcgttgag gtccttggcc ttcttgccgg cgttggggat cttgtcggag gcgggggtgg
    10861 aagcgtctgt catgtcgtcc attctcactc gccgccggcg acgcccggcg tcagggtccc
    10921 gaggacctcg tcggcggccc ggtgcgcgga ggcgatgcac gccgggatgc cgacgccgtc
    10981 gtagagcgcg ccgcagaccc gcagcgggcc cggcagtccg gcgaccgcgg cccggatgcg
    11041 cgccacccgg tccacatggc cgacggcgta ctgcggcagc ccgccgtccc agcgggtgac
    11101 ggtggtggcc accggccggg cggtcaggcc gaccgcctcg ccgaggtcgg cgagggagag
    11161 ggcgaccagt tcggcgtcgt cgcgctccag atcggcctcg tcgccgtacc ggccgaggga
    11221 ggtgcgcagc acgaagaggt ccgggtcggc ctcggcgatc cagttccact tgtggctggc
    11281 gaaggtcgat gccttga
//
