LOCUS       NZ_LJSN01000003        10990 bp    DNA     linear   CON 29-MAR-2024
DEFINITION  Streptomyces noursei strain JCM 4701 scaffold3, whole genome shotgun
            sequence.
ACCESSION   NZ_LJSN01000003
VERSION     NZ_LJSN01000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces noursei
  ORGANISM  Streptomyces noursei
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 3830602)
  AUTHORS   Graham,D.E., Mahan,K.M., Klingeman,D.M., Fida,T., Giannone,R.J.,
            Hettich,R.L., Parry,R.J. and Spain,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-SEP-2015) Biosciences Division, Oak Ridge National
            Laboratory, PO Box 2008, MS-6038, Oak Ridge, TN 37831-6038, USA
REFERENCE   2  (bases 1 to 3830602)
  AUTHORS   Graham,D.E., Mahan,K.M., Klingeman,D.M., Fida,T., Giannone,R.J.,
            Hettich,R.L., Parry,R.J. and Spain,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-SEP-2015) Biosciences Division, Oak Ridge National
            Laboratory, PO Box 2008, MS-6038, Oak Ridge, TN 37831-6038, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2015
            Assembly Method :: SPAdes v. 3.6; SMRT Analysis v. 2.3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300.0x
            Sequencing Technology             :: Illumina MiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002891315.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 20:38:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,947
            CDSs (total)                      :: 8,851
            Genes (coding)                    :: 8,489
            CDSs (with protein)               :: 8,489
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 362
            CDSs (without protein)            :: 362
            Pseudo Genes (ambiguous residues) :: 0 of 362
            Pseudo Genes (frameshifted)       :: 118 of 362
            Pseudo Genes (incomplete)         :: 301 of 362
            Pseudo Genes (internal stop)      :: 23 of 362
            Pseudo Genes (multiple problems)  :: 74 of 362
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:29:39
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 940998
            Orig. end                         :: 951988
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LJSN01000003.1.
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: http://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10990
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="7"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10990
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10990
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5990](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5990
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(392..405)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.78"
                     /tool="antismash"
     gene            474..1178
                     /locus_tag="AOB60_RS25760"
                     /old_locus_tag="AOB60_24740"
     CDS             474..1178
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018537752.1"
                     /locus_tag="AOB60_RS25760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOB60_24740"
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="WP_102925182.1"
                     /transl_table=11
                     /translation="MPLEALRPSPLVEQAAQQLRDQITGGHWPVGSRLPGETTLAKTLG
                     VGRSTVREALRALAGAGMVQPRQGAGVFVIAAEPAEDWPTRLRRAAVADVYEVRAMVEV
                     QAARLAAERRTEADVTALYAALERRRADGADDAAFVDADIALHAAVVDAAHNPVLTDLF
                     GQFVAALRQGLIDLVRLLGLRDHDPHHGDARHAALVEAVAAGDADTAGDVLQSELAETR
                     ALLLTGRPAEGG"
     gene            complement(1313..3697)
                     /locus_tag="AOB60_RS25765"
                     /old_locus_tag="AOB60_24745"
     CDS             complement(1313..3697)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018537753.1"
                     /locus_tag="AOB60_RS25765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOB60_24745"
                     /product="MFS transporter"
                     /protein_id="WP_102925183.1"
                     /transl_table=11
                     /translation="MTQTMTDQLPAGAVDSAAALPLPPPAKGGHRRTHAGIVPVLAFAG
                     IVVAVMQTLLVPVIKDLPTLLGTAPSNATWVMTATLLAGAVATPIMGRLGDLYGKRRML
                     LASLAVMVVGSLICGFTDQLIVMIVGRALQGFAMGAIPLGIGLMRDELPRERLGSAMAL
                     MSSSIGVGGGLALPAAALVAQHADWHTLFFAAAGLGVLSMLLTALVVPETSVRAPGSFD
                     VAGALGLSVGLVALLLPITKASEWGWGSPRTLGLFGAAVLILVLWGVMELRIADPLVDL
                     RTTARREVLLTNLASITVGVAFYAISLVLPQLLQLPTSTGYGLGQSMVVAGLCVAPLGL
                     TMMFVAPLYARIAARRGPKVSLLLGMLVIGVGYGGGIGLMQAPWQTVIIAVVVGAGIGL
                     AYSSLPALIIGAVDPSETGAANGLNTLMRSIGTSVSSAVIGMVLARMSEPLGPITVPTM
                     AGFRVSFLIATAAVALGVLVAAFLPPQRKAAGARTTPVTQSADEADTAADVPADAAGRS
                     APVAGAADEPTAGFRGRVVDTAGGPVPGATVTLIDRHGQQAGVTTAAPDGSYALAAPVA
                     GTYVLTGAAPAHAPQAVSAAHAGEGTVAGADLVLTPAGSCLAGTVLGGPEGAALAGARV
                     VITDAHGDVVTHTTTGADGRWRVAQLPDGPYTLVFSAPGHQPTAHAVQLGGGVDGAGGD
                     TRHQEVRLRPGGTVRGTVRGPDGHPLADAAVTLLEDGTVAGHAVTGPDGAYAFADLVGR
                     HYTLSAAGYPPHAAPISLAGGADARLDLELAQPGGAGQRER"
     misc_feature    3841..3856
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.26"
                     /tool="antismash"
     gene            complement(4130..4828)
                     /locus_tag="AOB60_RS25770"
                     /old_locus_tag="AOB60_24750"
     CDS             complement(4130..4828)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092050.1"
                     /locus_tag="AOB60_RS25770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOB60_24750"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_073445479.1"
                     /transl_table=11
                     /translation="MARQDRAIKTRRVILESAAAVFDEHGFDRATIAEILSRAGVTKGA
                     LYFHFPSKEDLALAVLHEQVLDIAVRPQPIKLQEFVDAGQILAYRLRHDPIQRGAARLA
                     IEPGANQLDRKQSMSAWTTFVEGLLNEARARGEVLESVNVRDTAELFVGAFAGIQMMSK
                     AFTDQNDVSHRLLVFFEHTLPTIAVPAILAKLRLDPERGARLDAELRERAAEEAERAAA
                     LEADLQNAAP"
     misc_feature    4926..4939
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.86"
                     /tool="antismash"
     gene            5001..5990
                     /locus_tag="AOB60_RS25775"
                     /old_locus_tag="AOB60_24755"
     CDS             5001..5990
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608008.1"
                     /locus_tag="AOB60_RS25775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOB60_24755"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_258018092.1"
                     /sec_met_domain="AfsA (E-value: 5e-20, bitscore: 69.0,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDTLQASSTVPVTSRRRMQQVQNPPAASRRGSDTSAPIPHEFLH
                     RPLVEDILIISWLRLSQDRFSVTAQWPHDHLYFAPHDSRPHPMLAGETIRQAGLLLCHT
                     EFGVPLGHHFILHDLVYTADPAHRVSGNAPTQLSIDVVCSRTRMRAGMLSSSRFDMTIR
                     KDGRVVATGHSHVSVTSPAVYRRVRGERITARHVPGPLPTPAPPGLTGSATERDVLLSP
                     AGQPGRWLLRIAPGHPTVVNPANDHVPGLSLLDAAQQAARALTGSSSFLPHAFGTEFHR
                     YAEHGRPCLIEARRKVPTATGTTTVLVTGAQDGDPVFASVLTALDARS"
     gene            6208..6972
                     /locus_tag="AOB60_RS25780"
                     /old_locus_tag="AOB60_24760"
     CDS             6208..6972
                     /NRPS_PKS="Domain: PKS_KR (7-177). E-value: 7.9e-08. Score:
                     24.3. Matches aSDomain:
                     nrpspksdomains_AOB60_RS25780_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608009.1"
                     /locus_tag="AOB60_RS25780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOB60_24760"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_102925185.1"
                     /transl_table=11
                     /translation="MGDLYGKTALVTGSSRGIGRGIAQRLAQDGAVVAVHYGSNEGAAK
                     ETVEGIRDNGGRAFLIGAELGVPGDAEALFDSFDAGLATAGAEPGLDILVNNAAISLPG
                     HIGEVTTEEFDRTIAINTKAPFFIIQHGLRRMRDGGRIVNISSAVTSTAFPSTIAYGLS
                     KGAIDTLTRTLAKDVGDRGITVNTVAPGFIETDMNAAMRSTPEAHAALAAISVFNRLGR
                     PADVADVVAFLASDDSRWITGQRIDVTGGSML"
     aSDomain        6229..6738
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AOB60_RS25780_PKS_KR.1"
                     /evalue="7.90E-08"
                     /label="AOB60_RS25780_PKS_KR.1"
                     /locus_tag="AOB60_RS25780"
                     /protein_end="177"
                     /protein_start="7"
                     /score="24.3"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="TALVTGSSRGIGRGIAQRLAQDGAVVAVHYGSNEGAAKETVEGIR
                     DNGGRAFLIGAELGVPGDAEALFDSFDAGLATAGAEPGLDILVNNAAISLPGHIGEVTT
                     EEFDRTIAINTKAPFFIIQHGLRRMRDGGRIVNISSAVTSTAFPSTIAYGLSKGAIDTL
                     TRTLAKD"
     CDS_motif       6235..6300
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AOB60_RS25780_0001"
                     /evalue="6.70E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="AOB60_RS25780"
                     /protein_end="31"
                     /protein_start="9"
                     /score="12.4"
                     /tool="antismash"
                     /translation="LVTGSSRGIGRGIAQRLAQDGA"
     gene            complement(7036..7545)
                     /locus_tag="AOB60_RS25785"
                     /old_locus_tag="AOB60_24765"
     CDS             complement(7036..7545)
                     /EC_number="2.1.1.63"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608011.1"
                     /locus_tag="AOB60_RS25785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOB60_24765"
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="WP_102925186.1"
                     /transl_table=11
                     /translation="MTLYATLDSPLGELLLVGERSATAPGGTALASLSVPGQKRGAVVQ
                     DDWTHDPAAFAGIARQLRAYFDGAQQPFAVECVPRGTDFQQRVWAALEAIPYGTTTTYG
                     AIAAAIGASRGAVRAIGTAIGANPLLVVRPCHRVIGADGSLTGYAGGLARKQLLLGLEG
                     AVPEAR"
     gene            complement(7542..9020)
                     /locus_tag="AOB60_RS25790"
                     /old_locus_tag="AOB60_24770"
     CDS             complement(7542..9020)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018537758.1"
                     /locus_tag="AOB60_RS25790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOB60_24770"
                     /product="DNA-3-methyladenine glycosylase 2 family protein"
                     /protein_id="WP_102925187.1"
                     /transl_table=11
                     /translation="MSEDSRYEAVRSRDARFDGAFFFAVSTTGIYCRPSCPATTPRRRN
                     VTFFPTAAAAQGSGFRACRRCRPDAVPGSVEWNARADVVGRAMRLIGDGVVDREGVAGL
                     ADRLGYSARQVQRQLTAELGAGPAALARAQRAHTARVLLQTTGLPIAEIAFAAGFASVR
                     QFNDTIKEIYALTPSGLRAVRPGPGSRFGGLAAPSGTPGVLPLRLAYRGPYAARQIFDY
                     LAQRALGGIEEVTGEPGGRTYRRTLRLPHGTGIAEVDEATGDGWLDCRLHLTELRDLAT
                     AAQRVRRLFDLDADPFAVGERLGADPALAPLVARCPGLRSPGTVDPDELAVRAVLGRRA
                     GTAAGRALGTRLVAAHGEPLPRPAGTLGHLFPRAAELADAPLTGLGLSDARIAALRALA
                     AALAARDVVLDAGTDRDRAERALRGLPGIGPWTADYIRMRALGDPDVLLGAPPAGTDPA
                     VWSPWRSYATHHLWAADPAGTAERHPNPTERIDA"
     gene            complement(9207..9434)
                     /locus_tag="AOB60_RS25795"
                     /old_locus_tag="AOB60_24775"
     CDS             complement(9207..9434)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092057.1"
                     /locus_tag="AOB60_RS25795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOB60_24775"
                     /product="hypothetical protein"
                     /protein_id="WP_039629879.1"
                     /transl_table=11
                     /translation="MSEHPPVIVHPPTPSGGRRVTVHGQIVGLAHGRGDVAEFLRRAGV
                     AGPAEDIALDDPRLVEWRGGSLDDWPMPSP"
     gene            9618..10868
                     /locus_tag="AOB60_RS25800"
                     /old_locus_tag="AOB60_24780"
     CDS             9618..10868
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018537760.1"
                     /locus_tag="AOB60_RS25800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOB60_24780"
                     /product="cytochrome P450"
                     /protein_id="WP_102926345.1"
                     /sec_met_domain="p450 (E-value: 1.6e-23, bitscore: 80.8,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MESAEFSRNPYPLLAALRAGGPVQRVRTGKDRTTWVVTGWAEARA
                     ALADRRLSKDTARYFADRPSGRDLAPAVSRSMLATDPPDHGRLRKLAMTAFTPAAVSRL
                     EPRIREIAEGLATALAGTAQAAGGGPVDLVEGFAAPLPITVICELLAVPDPDRAAVRRW
                     SDDLFAAADPDTADRASHALAGYLTELIAARRAAPGDDVLSGLIAARDAGDRLSEPELV
                     SLAVLLVVAGHETTANLIGNGLLALLRDDALRARLRDDAGLLPAAVEEFLRFDAPVTLA
                     TFRYATEPFDLGGARIEAGDVVMVSPGGANRDPARFDEPDAVRLDRPAPGGHVSFGHGP
                     HHCLGAPLARAEARIAFAVLLARFPALRLATDGRDASDGPDGVSWRRTRLMRGPARLPA
                     LLGPIAGGSNGAPPARR"
ORIGIN
        1 ctccttgaag cccatggcga ccagcaggtc gaacagccgg cgcttgcgcg gggtgtccat
       61 cggttcggcc agcgcctggt tgccgtcgcg caggtcgacc ggcacccaca ggggcgcccg
      121 ctcgatccgg gcggcgggcc acgcgcggtc ggtgtgcggc acctcgaccc gctggtgggc
      181 gggccggtag cgatgggccg gcatgtcact gccgcgctgc ggattccagg ccggggccgt
      241 cgcgggcacc ggcccgcggg gcgtgcggac ggtggggaaa gtgcgggaca tcgcgttcct
      301 tcacagaagc cggggggaga gaccggcagc accgaacccc gcggcggggt gccggtcgtg
      361 tcaggccccg ccgcggcacc cgagaaggag gccacgcagc gtcatggcgg gtacactaac
      421 cacgaatcaa ctcctcagac aagctgagga gtcatgagga caggagaaac gggatgccgc
      481 tggaagccct ccgccccagc cccctggtcg aacaggccgc gcaacagctg cgcgaccaga
      541 tcaccggcgg acactggccg gtgggcagca ggctcccggg cgagacgacg ctggccaaga
      601 ccctcggcgt cggccgctcg acggtccgcg aagcgctgcg cgcgctggcc ggcgccggaa
      661 tggtgcagcc ccgccagggc gccggcgtct tcgtcatcgc cgccgaaccc gccgaggact
      721 ggccgacccg cctgcgccgc gcggccgtcg ccgacgtcta tgaggtgcgc gcgatggtcg
      781 aggtccaggc cgcccgcctc gccgccgagc gccgcaccga ggccgatgtc acggccctgt
      841 acgcggcact ggagagacgg cgggcggacg gcgccgacga cgcggccttc gtcgacgcgg
      901 acatcgccct gcacgcggcg gtggtggacg cggcccacaa ccccgtgctg accgacctct
      961 tcggccagtt cgtcgccgcc ctgcgccagg gcctgatcga cctcgtccgg ctgctcggcc
     1021 tgcgcgacca cgacccgcac cacggggacg cccgccacgc cgccctcgtc gaggccgtgg
     1081 ccgccggtga cgccgacacc gccggcgacg tcctccagtc cgaactggcg gaaacccggg
     1141 cgctcttgct gacggggcgc cccgcggaag gcgggtgaca cctcgtcagg aatgcgcgat
     1201 cgccgctggc agcggtgccg ccggcactac ctgcgtcggc ccggccgtcg gcagtggccc
     1261 tccgcgctga cgaggaatca ccgcgggccg gcgcacacca cgggcggcac tctcagcgct
     1321 cccgctgccc ggccccaccc ggctgcgcca actccaggtc cagccgcgca tccgcgccgc
     1381 ccgccaggga tatcggcgcg gcgtgcggcg gatagccggc cgccgagagg gtgtagtggc
     1441 gtcccaccag gtcagcgaag gcataggcgc cgtccggccc ggtgaccgca tgcccggcga
     1501 ccgtcccgtc ctccagcagg gtcacggcgg cgtccgccaa ggggtgcccg tccggcccgc
     1561 ggaccgtacc gcgtacggtg ccgccgggcc gcagccgcac ctcctggtgg cgcgtatcgc
     1621 ccccggcgcc gtcgacgccg ccgcccaact ggacggcatg cgcggtcggc tggtgccccg
     1681 gagcgctgaa gacgagggtg tagggcccgt cgggcagttg ggcgaccctc caccgcccgt
     1741 cggctccggt cgtcgtgtgc gtcaccacgt cgccgtgggc atcggtgatc acgacgcgcg
     1801 ctccggccag cgccgcccct tccgggccgc cgagcacggt gccggcgagg cagctgccgg
     1861 cgggcgtcag caccaggtcc gccccggcca ccgtgccctc gccggcatgc gcggcggaga
     1921 cggcctgcgg ggcgtgggcc ggggcggcgc cggtcaggac gtatgtgccc gcgaccgggg
     1981 cggccagcgc ataactgccg tcgggcgcgg ccgtggtgac cccggcctgc tgcccgtgcc
     2041 ggtcgatcag ggtgaccgtg gcgcccggca ccgggccgcc tgcggtgtcc acgacccgcc
     2101 cccggaaccc ggcggtcggc tcgtccgccg ctcccgcaac gggtgcactc cgcccggccg
     2161 cgtccgccgg gacgtccgcc gctgtgtcgg cctcgtccgc gctctgcgtc accggcgtcg
     2221 tccgcgcccc ggcggccttc cgctggggcg gcaggaaggc cgccaccagc accccgaggg
     2281 cgaccgcggc ggtggcgatc aggaacgaga cccggaaccc ggccatcgtg ggcaccgtga
     2341 tcggccccag cggctcggac atacgggcca gcaccatgcc gatgacggcg ctggacacgg
     2401 acgtgccgat cgaacgcatc agggtgttca ggccgttggc cgcgccggtc tcggacgggt
     2461 cgacggcccc gatgatgagc gctgggagcg aggagtacgc caggccgatg cccgcgccga
     2521 ccaccaccgc gatgatgacc gtctgccagg gggcctgcat caggccgatc ccgccgccgt
     2581 aaccgacccc gatgaccagc atgccgagca gcagcgagac cttggggccg cggcgggccg
     2641 cgatccgtgc gtacagcggt gccacgaaca tcatcgtcag ccccagcggc gccacacaca
     2701 gcccggccac caccatcgac tggccgaggc cgtagccggt cgacgtgggc agctggagca
     2761 gctgcggcag caccagcgag atggcgtaga acgccactcc gaccgtgatg gaggcgaggt
     2821 tggtgagcag cacctcgcgc cgggcggtgg tgcgcaggtc caccagcggg tcggcgatcc
     2881 gcagctccat cacgccccac agcaccagga tcagcaccgc ggccccgaac aggccgaggg
     2941 tgcggggcga tccccaaccc cactcgctcg ccttggtgat cggcaacagg agggcgacca
     3001 ggccgaccga caggccgagg gcgccggcga cgtcgaagct gccgggcgcc cgcaccgagg
     3061 tctccgggac gaccagcgcg gtgagcagca tcgacagcac gccgagcccg gcggcggcga
     3121 agaacagcgt gtgccagtcg gcgtgttggg ccaccagggc cgcggcgggc agcgccagtc
     3181 cgccgccgac gccgatcgag gagctcatca gggccatggc ggagccgagc cgctcccggg
     3241 gcagttcatc gcgcatcagg ccgatgccga ggggtatcgc gcccatcgcg aagccctgca
     3301 gcgcccgccc gacgatcatg acgatcaact ggtcggtgaa gccacagatc agcgagccca
     3361 cgaccatgac ggcgagactc gccagcagca tccgccgctt gccgtagagg tcgccgaggc
     3421 ggcccatgat gggggtggcg accgcaccgg cgaggagggt ggccgtcatg acccaggtgg
     3481 cgttgctggg cgcggtgccg agcagggtcg gcaggtcctt gatgaccggc acgagcagtg
     3541 tctgcatcac cgcgacgacg atgccggcga aggcgagcac cgggacgatg ccggcgtgcg
     3601 ttctgcggtg tccgcccttg gcgggcgggg gcagtggcag cgcggcggcg gagtccacag
     3661 cccccgcggg gagctggtcc gtcatcgtct gggtcatgcg tgcggcctcc agggcggcag
     3721 gagcgagcgg agagggtgcg cggcgcgcga acaggggcgt ggccccttga tacgtgcatg
     3781 gcgaacgttt cagctcgggg gcctattcca agccacccgg ccagatctcg ggctccggac
     3841 ggtgatctcg ctcacgccga ccacgcccgg cctccgcctg taccaactcc cgtatgcggc
     3901 ccgccccttc gggcctaccg tcccgtccat cgcccctccc ccggtctcgt tccggacacg
     3961 ggatccagtg aaccgcacac cactgacaat cgaccggccg agggatcaag actgggtcaa
     4021 gagcggagca cgggcagcga ggccgcgccc gggacggaac cgacgtatca gcacacccgg
     4081 ggaacttccg ccccgcgaca tccgcacccc gaggagctcc cgcggcgcgc tacggcgccg
     4141 cgttctgcag gtccgcctcc agcgcggcgg cgcgctccgc ctcctccgcc gcgcgttccc
     4201 ggagctccgc gtcgagccgc gcaccccgct ccgggtcgag tctgagtttg gcgagaatgg
     4261 ccggcaccgc gatggtgggc agggtgtgct cgaagaagac caacagccga tgactgacgt
     4321 cgttctggtc ggtgaacgcc ttggacatca tctgaatgcc tgcgaaggca ccgacgaaca
     4381 gctccgccgt atcccgcacg ttgacgctct ccagcacttc gccccgcgcc ctggcctcgt
     4441 tgagcagtcc ctcgacgaac gtggtccacg ccgacatcga ctgcttgcga tcgagctggt
     4501 tggcacccgg ctcgatggcg agacgggcgg cgccgcgctg gatcgggtcg tgtcgcagcc
     4561 ggtatgccag gatctgcccg gcgtccacga actcctggag tttgatcggc tgcggccgca
     4621 cggcgatgtc gagcacctgc tcatgcagca cggcgagcgc aagatcttct ttagagggaa
     4681 agtggaaata caaggcaccc ttcgtcacgc cggcgcgcga gaggatctcg gcaatcgtcg
     4741 cgcgatcgaa gccgtgttca tcgaagaccg ccgcggcgga ttccaagatc actcgccgcg
     4801 tcttgattgc gcggtcctga cgtgccacag agcgcctcct gtctgtcaaa ggctcaagct
     4861 tctcagcatt tcgccacagc tcaagtctcc tccagatcgg cggatcgcct ttaccctcac
     4921 accggcattg aaaagaaaac cggatggtac gtatcttaat cgtgtccgga gctcttccgt
     4981 agagacgcat aggggctgtc atgagcgaca cgttgcaggc cagcagcacg gttccagtca
     5041 cgagccgacg ccggatgcag caggtccaga accccccggc cgcaagccgg cgggggtccg
     5101 acaccagtgc cccgatcccc cacgagtttc tgcaccgccc tctcgtggag gacatcctga
     5161 tcatttcctg gctccgactg agccaggacc gcttctccgt gaccgcccag tggccgcacg
     5221 atcacctcta cttcgccccc cacgacagcc ggccccatcc gatgctggcg ggagagacca
     5281 tccgacaggc cggactgctg ctctgccaca ccgagttcgg ggtaccgctc ggtcatcact
     5341 tcatccttca cgacctcgtc tacaccgccg accccgcaca ccgcgtctcc gggaacgcgc
     5401 cgacgcagct gagcatcgac gtcgtgtgca gccggacacg gatgcgcgcc ggaatgctgt
     5461 ccagcagccg cttcgacatg acgatccgca aggacggccg cgtcgtcgcg accggccact
     5521 cccatgtcag cgtcacctcg ccggccgtct accgccgggt ccgcggggaa cggatcacgg
     5581 cccgccacgt ccccggcccg ctccccaccc ccgccccgcc gggactgacc ggcagcgcca
     5641 ccgagaggga cgtcctgctc tcccccgccg ggcaaccggg ccgatggctg ctgcggatcg
     5701 cacccggcca ccccacggtc gtcaaccccg ccaacgacca cgtgcccggc ctgtccctgc
     5761 tcgacgcggc gcagcaggcc gcccgcgcgc taaccgggag cagctccttc ctgccccacg
     5821 ccttcggcac cgagttccac cgttacgcgg aacacggtcg cccgtgtctg atcgaggcgc
     5881 ggcggaaggt gccgacggcc accggcacca cgacggtcct ggtcaccggg gcccaggacg
     5941 gcgaccctgt cttcgcctcc gtcctcacgg ccctggatgc gcgcagctga ccggacgggc
     6001 gggccgccac cgaaccgccc gtccgagccc accgccgcat gagcgccggc gcgcacgcgc
     6061 cacccccctt cgtcatgagc ccgaccggct catgcaccgt gtaggcagac gacataccgg
     6121 aacaacggcc gggccgcccg cacagcactc ccctttgaca cggcggcaac cgaccgccag
     6181 aagcactacg gaaggccgac aacgaccatg ggtgaccttt acggaaagac cgcgctggtt
     6241 acgggatcca gccgcggcat cgggcgaggc atcgcccagc gcctcgcgca ggacggtgcc
     6301 gtggttgccg tccactacgg gagcaacgag ggcgcggcca aggagaccgt cgagggcatc
     6361 cgggacaacg gcggacgggc gttcctgatc ggcgcggagc tgggcgtccc gggcgacgcc
     6421 gaggcgctgt tcgactcctt cgacgccggg ctggccacgg cgggcgcgga acccggcctg
     6481 gacatcctcg tcaacaacgc ggcgatcagc ctgccggggc acatcggcga ggtgacgacc
     6541 gaggagttcg accggaccat cgcgatcaac accaaggccc cgttcttcat catccagcac
     6601 ggtctgcggc ggatgcggga cggcgggcgg atcgtcaaca tctcgtccgc ggtgacgagt
     6661 acggccttcc cctcgaccat cgcctacgga ctctccaagg gcgcgatcga caccctcacc
     6721 cggaccctcg ccaaggacgt cggcgaccgc gggatcacgg tgaacaccgt ggcgccggga
     6781 ttcatagaga ccgatatgaa cgcggcgatg cggtcaactc ccgaggcaca tgcggcactt
     6841 gccgccatct cggtgttcaa ccggctcggc cgccccgccg acgtcgcgga cgtggtcgcc
     6901 ttcctcgcct ccgacgactc ccgttggatc accggccagc gcatcgacgt caccggcggg
     6961 tcgatgctct gaccgacccg gtccgccggt ccgggtgagt ccccggaccg gcgggcgcgg
     7021 ccgtgttcgg ccggctcaac gggcctccgg caccgcccct tcgaggccga ggagcagttg
     7081 cttacgggcc aggccgccgg cgtagccggt gagcgacccg tcggcgccga tgacgcggtg
     7141 gcagggccgg accaccagca gcgggttggc gccgatcgcc gtcccgatgg cgcggaccgc
     7201 accgcgcgac gccccgatgg cggcggcaat cgctccgtag gtggtggtcg tgccgtacgg
     7261 gatggcctcc agcgccgccc agacacgctg ttggaagtcc gtgccgcggg ggacgcactc
     7321 gacggcgaac ggctgctgcg ccccgtcgaa gtacgcccgg agctggcggg cgatcccggc
     7381 gaacgcggcc gggtcgtgcg tccagtcgtc ctggacgacg gccccgcgct tctggcccgg
     7441 cacggagagc gaggcgagcg ccgtgccgcc gggtgcggtg gcggaccgct cgccgaccag
     7501 caggagttcg cccagggggc tgtcgagggt ggcgtacaag gtcatgcgtc gatccgttct
     7561 gtcgggttcg ggtgccgttc cgcggtgccg gcggggtcgg cggcccagag gtggtgcgtc
     7621 gcgtacgagc gccaggggct ccacactgcc gggtcggtgc ccgccggcgg tgcgccgagg
     7681 aggacatcgg ggtcgcccag cgcccgcatc cggatgtagt cggcggtcca cggaccgatg
     7741 ccggggagcc cgcggagcgc gcgttcggcc cggtcgcggt cggtcccggc gtccaggacg
     7801 acgtcccggg cggcgagggc ggcggcgagg gcgcgcagcg cggcgatccg cgcgtcggac
     7861 aggccgagcc cagtgagcgg cgcgtccgcc aactcggcgg cccgcgggaa gagatggcca
     7921 agggtgccgg caggccgtgg cagcggctcc ccgtgcgcgg cgaccaggcg tgtgcccagg
     7981 gcccggccgg cggcggtccc ggcccgccgg ccgaggacgg cgcgcaccgc gagttcgtcc
     8041 gggtccacgg tccccgggga gcgcaacccc gggcagcggg cgaccaaagg ggccagcgcg
     8101 ggatccgcac cgaggcgttc gccgaccgcg aacgggtcgg cgtccaggtc gaacaggcgg
     8161 cggacgcgct gggcggcggt ggcgaggtcc cgcaactcgg tgaggtgcag tcggcagtcc
     8221 agccagccgt cgccggtggc ctcgtcgacc tccgcgatgc cggtgccgtg gggcagccgc
     8281 agggtgcgcc ggtacgtccg gccgccgggc tccccggtga cctcctcgat cccgccgagc
     8341 gcccgctgcg ccaggtagtc aaagatctgt cgggccgcgt acgggccgcg gtacgccaac
     8401 ctcagcggca gcacgccggg cgtgccggac ggggccgcaa ggccgccgaa gcgggaaccg
     8461 ggcccggggc ggaccgcccg cagtccgctc ggggtgagcg cgtagatctc cttgatggtg
     8521 tcgttgaact gccggacgct ggcgaatccg gcggcgaagg cgatctcggc gatcggcagg
     8581 ccggtggtct gcaacagcac ccgcgcggtg tgcgcgcgct gcgcccgggc cagggcggcg
     8641 ggcccggcgc cgagctcggc ggtcagctgc cgctgcacct gccgggcgct gtagccgagc
     8701 cggtcggcca gcccggcgac gccctcccgg tcgaccaccc cgtcgccgat cagccgcatc
     8761 gcccgcccca cgacatccgc ccgggcgttc cactcgaccg agcccgggac cgcgtccggg
     8821 cggcaccgcc ggcaggcccg gaatccggag ccctgcgcgg ccgcggcggt cgggaagaag
     8881 gtgacgttcc gccgccgcgg ggtggtcgcg gggcagctcg gccggcagta gatccccgtc
     8941 gtcgacaccg cgaagaagaa cgccccgtcg aacctggcgt cccggctccg caccgcctcg
     9001 tacctgctgt cctcgctcat cacaccccca ctgtgcgtca tcccgcgcca ccgctctggc
     9061 ggaaatcgga cacggcgctg gcggcggcca ggaggaccac gtcgagcgac aacgggtgcc
     9121 cacggatgcg tgcggggggc cggcgtgggc tcgggggggg tggaggacgg gccgggcgcg
     9181 tctcggaggt gccaggccga ggcccgtcac ggcgacggca tcggccagtc gtccagggag
     9241 ccgccgcgcc actcgaccag gcgggggtcg tccagtgcga tgtcctcggc ggggccggcg
     9301 acacccgcgc gccggaggaa ctccgccacg tcgccgcggc catgggccag gccgacgatc
     9361 tgcccgtgca cggtcacccg gcgtccaccg gagggcgtcg gtgggtgcac gatgacaggc
     9421 ggatgctcgg acatgcctcc agcgtgcgcc gggctccgcg cgctcgcacc tcgacggtgg
     9481 gatctcaccc ggacggctgg gatccggacc ggacggggcg accggcgccg gtgcgaaggt
     9541 ggtcaccggg ccggtccggg aggtcgggcg cggacctgac cgcgaacgag agcacgaggg
     9601 tggcagtgaa caaccccatg gagtcggccg agttctcccg caatccctac ccgctgctcg
     9661 ccgcgcttcg ggcgggcggc ccggtgcagc gggtgcggac cggcaaggac cgcacgacgt
     9721 gggtggtgac cggctgggcg gaggcccgcg cggcgctggc cgaccggcgg ttgtcgaagg
     9781 acaccgcacg gtacttcgcg gatcggccct ccggacgcga tctggcaccg gccgtcagcc
     9841 ggagcatgct ggccaccgac ccgccggacc acggccggct gcggaaactg gccatgacgg
     9901 ccttcacccc cgcggcggtg agccggctgg agccccgcat ccgggagatc gcggaggggc
     9961 tggccacggc gctggccggg accgctcagg cggcgggcgg cggccccgtc gatctggtcg
    10021 aggggttcgc ggcgccgctg ccgatcacgg tgatctgcga gctgttggcg gtgccggacc
    10081 cggaccgggc cgcggtgcgc cggtggtccg acgacctgtt cgcggcggcg gatccggaca
    10141 ccgccgaccg ggcctcgcac gccctggccg ggtacctgac cgagttgatc gcggcgcggc
    10201 gcgcggcgcc cggggacgac gtgctgagcg gcttgatcgc cgcccgggac gccggtgacc
    10261 ggctcagcga gcccgaactc gtctcgctgg ccgtgctgtt ggtggtcgcc gggcacgaga
    10321 ccacggccaa tctgatcggc aacggcctgc tggccctgct ccgcgacgac gcgctgcgcg
    10381 cccgcctgcg cgacgacgcg gggctgctgc ccgccgcggt ggaggagttc ctgcggttcg
    10441 acgcgccggt gacgctggcg accttccgtt acgccaccga gccgttcgac ctcggcggcg
    10501 cccgcatcga ggcgggggac gtggtcatgg tctcgcccgg cggcgccaac cgcgacccgg
    10561 cgcggttcga cgaaccggac gcggtacggc tggaccgccc ggccccgggc ggccacgtgt
    10621 cgttcgggca cggaccgcac cactgcctgg gcgcgccact ggcccgggcc gaggcccgga
    10681 tcgcattcgc ggtactactc gcccgcttcc ccgcactgcg actcgccacg gacggccggg
    10741 acgcctccga cggccccgac ggggtcagtt ggcgacggac ccggctcatg cgcggcccgg
    10801 cccggctccc ggcgcttctg ggtccgatcg cgggcggctc gaacggggcg ccgccggcgc
    10861 ggcgttgagg cgaggcggct cgcgcacggc ccggcccggc cgttcggcat gcgacgaccc
    10921 tcttcaaggc accggcgccg cgaccgcagt gcggcggtcg cggcgccggt cgtccatggc
    10981 ccgtcgcggt
//
