LOCUS       NZ_LJSN01000002        10405 bp    DNA     linear   CON 29-MAR-2024
DEFINITION  Streptomyces noursei strain JCM 4701 scaffold2, whole genome shotgun
            sequence.
ACCESSION   NZ_LJSN01000002
VERSION     NZ_LJSN01000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces noursei
  ORGANISM  Streptomyces noursei
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 4596274)
  AUTHORS   Graham,D.E., Mahan,K.M., Klingeman,D.M., Fida,T., Giannone,R.J.,
            Hettich,R.L., Parry,R.J. and Spain,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-SEP-2015) Biosciences Division, Oak Ridge National
            Laboratory, PO Box 2008, MS-6038, Oak Ridge, TN 37831-6038, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2015
            Assembly Method :: SPAdes v. 3.6; SMRT Analysis v. 2.3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300.0x
            Sequencing Technology             :: Illumina MiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002891315.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 20:38:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,947
            CDSs (total)                      :: 8,851
            Genes (coding)                    :: 8,489
            CDSs (with protein)               :: 8,489
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 362
            CDSs (without protein)            :: 362
            Pseudo Genes (ambiguous residues) :: 0 of 362
            Pseudo Genes (frameshifted)       :: 118 of 362
            Pseudo Genes (incomplete)         :: 301 of 362
            Pseudo Genes (internal stop)      :: 23 of 362
            Pseudo Genes (multiple problems)  :: 74 of 362
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:29:39
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3006449
            Orig. end                         :: 3016854
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LJSN01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="17"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5405
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(192..1178)
                     /locus_tag="AOB60_RS14895"
                     /old_locus_tag="AOB60_14180"
     CDS             complement(192..1178)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536882.1"
                     /locus_tag="AOB60_RS14895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOB60_14180"
                     /product="SCO1860 family LAETG-anchored protein"
                     /protein_id="WP_102923835.1"
                     /transl_table=11
                     /translation="MSTRSTRFAGSTGTTGRIGAVLAAAVLTAGPAVALAAAPAAYATG
                     DDAHSHGTSGAVVLRTALDVNLLDKSLNVPVRAVLNEVHAPASADKTALTVRLDGIDGG
                     RPVSLLRADVATARATADGRTTEGHANLVRAKVQLPGLPALPLAEVQQVTARAVCTAGQ
                     HPRAESQVLGDVVVLGKRVALRAGGTTEVDVPGVGDVRLDLSQKSTTSASAAATALQLR
                     VSVNPLKLNVAEVHGQVTLAQATCTTPHGTPDKPGTPAHPGQPHEPGTGTQAHTGGGAR
                     PATENLAETGGSAATPYLIGGAVLLVAGGAGAMVYTRRRSAAPDGRG"
     gene            complement(1442..2533)
                     /locus_tag="AOB60_RS14900"
                     /old_locus_tag="AOB60_14185"
     CDS             complement(1442..2533)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016576771.1"
                     /locus_tag="AOB60_RS14900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOB60_14185"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_180990213.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 2.2e-16, bitscore:
                     57.9, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEGAVLHLKGRVLVGPDDVRDELWVIDGRVTFERPALARDARTV
                     TGWVLPGLVDAHCHVGLDAHGPVDAATSEKQALTDRDAGTLLIRDAGSPSDTRWIDDRP
                     DLPRIIRAGRHIARTRRYIRNYAHEIEPADLVAYVAQEARRGDGWVKLVGDWIDRSTGD
                     LAACWPPGEVAAAIAEAHRLGARVTAHCFAEESLAPLVEAGIDCIEHATGLTEDTIPLF
                     AERQVAIVPTLVNIATFPQLAAGGETKFPRWADHMRRLHARRYDTVRAAYDAGVPIFAG
                     TDAGGSLPHGLVADEVAELMKAGLPVEAALSATTWGAREWLGRPGLTEGASADLVVYET
                     DPRVDVRVLAAPRLVVLRGRVVG"
     misc_feature    2837..2851
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.87"
                     /tool="antismash"
     misc_feature    complement(2861..2876)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    complement(2871..2886)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.52"
                     /tool="antismash"
     misc_feature    2924..2936
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 16.37"
                     /tool="antismash"
     misc_feature    3005..3017
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     misc_feature    3028..3042
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.04"
                     /tool="antismash"
     gene            3042..3608
                     /gene="ectA"
                     /locus_tag="AOB60_RS14905"
                     /old_locus_tag="AOB60_14190"
     CDS             3042..3608
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536877.1"
                     /locus_tag="AOB60_RS14905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOB60_14190"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_180990141.1"
                     /transl_table=11
                     /translation="MTAAQADHAGARSEIREIPEGFKLDTPRVEDGAAIWRIARDSKTL
                     DLNSSYSYLLWCRDFAATSAVARDATGEPAAFITGYLRPDRPDTLVVWQVAVDAAHRGR
                     GLAAALLDGLTTRAVDELGVRRVETTISPGNTASNRLFASFAERHSVLIEREMLFDAAL
                     FPEQGHEAEVLHLIGPFEAPPGSRR"
     gene            3677..4945
                     /gene="ectB"
                     /locus_tag="AOB60_RS14910"
                     /old_locus_tag="AOB60_14195"
     CDS             3677..4945
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-365). E-value: 4.6e-74.
                     Score: 241.4. Matches aSDomain:
                     nrpspksdomains_AOB60_RS14910_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="AOB60_RS14910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOB60_14195"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_102923836.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.6e-76, bitscore:
                     255.5, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLTQPDLSVFETVESEVRSYCRGWPTVFDRAQGSRLTDEDGHTY
                     LDFFAGAGSLNYGHNNAVLKRALLDYIERDGITHGLDMSTTAKRAFLESFQNIILRPRD
                     LPYKVMFPGPTGANSVEAALKLARKVKSRESIVSFTNAFHGMSLGALAVTGNSMKRAGA
                     GIPLVHGTPMPFDNYLDGQTPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARS
                     EWLRALAALCQRQDMLLIVDDIQMGCGRTGAFFSFEEAGIVPDIVTVSKSISGYGLPMA
                     LTLFKPELDIWEPGEHNGTFRGNNPAFVTAAAALDTYWADGQMEKQTLARGEIVEAHLK
                     AIAEEHPGTFAEYRGRGLVWGLECTDKSLANKIAGRAFELGLLIETSGPQSEVVKLLPA
                     LTTTPEELDEGLRILARAVRDCA"
     aSDomain        3761..4771
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AOB60_RS14910_Aminotran_3.1"
                     /evalue="4.60E-74"
                     /label="AOB60_RS14910_Aminotran_3.1"
                     /locus_tag="AOB60_RS14910"
                     /protein_end="365"
                     /protein_start="28"
                     /score="241.4"
                     /tool="antismash"
                     /translation="FDRAQGSRLTDEDGHTYLDFFAGAGSLNYGHNNAVLKRALLDYIE
                     RDGITHGLDMSTTAKRAFLESFQNIILRPRDLPYKVMFPGPTGANSVEAALKLARKVKS
                     RESIVSFTNAFHGMSLGALAVTGNSMKRAGAGIPLVHGTPMPFDNYLDGQTPDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARSEWLRALAALCQRQDMLLIVDDIQMGCGR
                     TGAFFSFEEAGIVPDIVTVSKSISGYGLPMALTLFKPELDIWEPGEHNGTFRGNNPAFV
                     TAAAALDTYWADGQMEKQTLARGEIVEAHLKAIAEEHPGTFAEYRGRGLVWGLECT"
     gene            5001..5405
                     /locus_tag="AOB60_RS14915"
                     /old_locus_tag="AOB60_14200"
     CDS             5001..5405
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019358304.1"
                     /locus_tag="AOB60_RS14915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOB60_14200"
                     /product="ectoine synthase"
                     /protein_id="WP_039637583.1"
                     /sec_met_domain="ectoine_synt (E-value: 7.4e-52, bitscore:
                     172.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFQEIEGTDRHVKAKSGTWESKRIVLAKERVGFSLHETVLY
                     AGTETSMWYANHIEAVVCVEGVAELTNDETGEKHLITPGVMYLLDGHEKHTMRIKEDFR
                     CLCVFNPPVTGREDHDENGVYPLLTEPEAG"
     gene            5476..6378
                     /gene="thpD"
                     /locus_tag="AOB60_RS14920"
                     /old_locus_tag="AOB60_14205"
     CDS             5476..6378
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016577185.1"
                     /locus_tag="AOB60_RS14920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOB60_14205"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_073444977.1"
                     /sec_met_domain="PhyH (E-value: 3.8e-40, bitscore: 136.3,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAPERTADLYPTRGTSEVITPRKDPVVWSRPGVEGPFAAAELS
                     DFDRDGFCALPELLTADEVAVYRAELDRLVGDPAMRADPRSIVEPTSQAVRSVFEVHRI
                     SEVFAGLVADPRVAGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLP
                     HMRTVSVSIALTENYDTNGGLMIMPGSHRYFVGCEGATPKDNYKKSLQMQDAGTPSDEA
                     LTTMADRHGIRLFTGRAGSATWFDCNAMHGSGDNITPYPRSNVFIVFNSVENPAVEPFA
                     APVRRPEYIGARAPFVPVT"
     gene            complement(6520..7593)
                     /locus_tag="AOB60_RS14925"
                     /old_locus_tag="AOB60_14210"
     CDS             complement(6520..7593)
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016574047.1"
                     /locus_tag="AOB60_RS14925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOB60_14210"
                     /product="alkene reductase"
                     /protein_id="WP_073444978.1"
                     /transl_table=11
                     /translation="MTTAFDPIELAGIPLANRIVMAPMTRNRATAEGVPTPSMVAYYTQ
                     RASAGLIITEATQTEVVGRGYPLTPGLHTADQVAGWRRVTDSVHAAGGRIFAQLWHGGR
                     IGHPVLLPDGLEPVGPSAVKAAGQGFTQNGPTDFVTPRELTDEEIRATVDGFAAAARNA
                     IEAGFDGVEIHGANGYLIHQFLAPNANRRTDAWGGSDEARARFAIEVVKAVAGAVGAHR
                     TGLRLSPGNPFNDIDEPAPEATYTALIEAVAPLGLAYLHTCDVTDLELLARLRKRFDGP
                     FLLNPATEGRPTGPEELSLVERGEADLLAYGALFIANPDLPRRLAAGGPFNTPDRDGFY
                     GGDDRGYLDYPALDDEN"
     gene            complement(7724..8797)
                     /locus_tag="AOB60_RS14930"
                     /old_locus_tag="AOB60_14215"
     CDS             complement(7724..8797)
                     /NRPS_PKS="Domain: Aminotran_5 (16-348). E-value: 1.9e-19.
                     Score: 61.8. Matches aSDomain:
                     nrpspksdomains_AOB60_RS14930_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536872.1"
                     /locus_tag="AOB60_RS14930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOB60_14215"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_073444979.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.1e-11, bitscore:
                     42.2, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQRHMALGGDEFAPETVYLNSASCGLLPARSAAALTAAVAESASH
                     GTMGRDYLGTGARVRGAFARLMGVPEERVALGSSVAVQSAFVAGSLPAGSEVLVAEGDF
                     SSLVNPLAARAGCTVRAVPWEALADAVRPGTALVAVSAVHALDGRIADLAAIRDAARAH
                     GALTYVDITQAAGWLPLRLADFDYVVCGAFKWLLCPRGTTFMVFGGERGEPGGPGWPVP
                     AHAGWVAGEDPGESNYGPVDRPAATARRYDEPHAHYSYVAAEHSLGLLAELGVDTVHAH
                     NVALAQQYRAGLEAAGFTPRAAPGSAIVSTPGLADAEPRLARAGVRVSVRGGLLRAAFH
                     LYNSTEDVSRALSLLVP"
     aSDomain        complement(7754..8749)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AOB60_RS14930_Aminotran_5.1"
                     /evalue="1.90E-19"
                     /label="AOB60_RS14930_Aminotran_5.1"
                     /locus_tag="AOB60_RS14930"
                     /protein_end="348"
                     /protein_start="16"
                     /score="61.8"
                     /tool="antismash"
                     /translation="VYLNSASCGLLPARSAAALTAAVAESASHGTMGRDYLGTGARVRG
                     AFARLMGVPEERVALGSSVAVQSAFVAGSLPAGSEVLVAEGDFSSLVNPLAARAGCTVR
                     AVPWEALADAVRPGTALVAVSAVHALDGRIADLAAIRDAARAHGALTYVDITQAAGWLP
                     LRLADFDYVVCGAFKWLLCPRGTTFMVFGGERGEPGGPGWPVPAHAGWVAGEDPGESNY
                     GPVDRPAATARRYDEPHAHYSYVAAEHSLGLLAELGVDTVHAHNVALAQQYRAGLEAAG
                     FTPRAAPGSAIVSTPGLADAEPRLARAGVRVSVRGGLLRAAFHLYNSTEDV"
     gene            complement(8877..9572)
                     /locus_tag="AOB60_RS14935"
                     /old_locus_tag="AOB60_14220"
     CDS             complement(8877..9572)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536871.1"
                     /locus_tag="AOB60_RS14935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOB60_14220"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_102923837.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYIGKARFEAGLAAFAQRADVEVVHRSFELDP
                     AAPPATDVPILDMLATKYGVSREQAEAMEARVAEAAAGEGLGYRSDRIHGNTFDLHRLL
                     HLAKAHGVQDALLTALYHANFAEARQLADPAVLTGIAVGAGVPEAEAARVLADPDAYAD
                     EVRADERAAAELGATGVPFFVIDRRYGVSGAQPADVFRQALERAHADSAVQVLAADDAD
                     SCGDGSCAV"
     misc_feature    10175..10189
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 20.15"
                     /tool="antismash"
ORIGIN
        1 cgggaccggg cgaccaccga ccgcttcgtg gccgccctgg cggacgtgct cggcgacccg
       61 gcccggtaga gcgggcgggg tgcgccgcgc ggacccacgc ggcgcacccg tccccaccag
      121 cgcccggcgc cccccacggc accgatgcgc tggggatgcc tcccctgccg tgcgggcgag
      181 gggagggtcc gtcagccgcg cccgtccggc gcggcgctcc ggcggcgggt gtagaccatc
      241 gccccggcgc cgccggcgac cagcaggacc gcgccgccga tgaggtacgg ggtggcggcg
      301 ctgccgccgg tctcggcgag gttctcggtg gccgggcggg cgccgccccc ggtgtgcgcc
      361 tgggtgccgg tgcccggctc gtggggctga ccggggtgcg ccggggtgcc gggcttgtcc
      421 ggggtgccgt gcggggtggt gcaggtggcc tgggccagcg tgacctgacc gtggacctcc
      481 gcgacgttga gcttcaacgg gttgacggag acccggagtt ggagcgcggt ggcggcggcg
      541 ctcgccgagg tggtgctctt ctgcgacagg tcgagccgga cgtcgccgac gccgggcacg
      601 tcgacctcgg tggtcccgcc ggcacggagc gcgacccgct tgcccagcac cacgacgtcg
      661 cccagcacct gggactcggc ccgcggatgc tgtccggccg tgcagacggc gcgggcggtg
      721 acctgctgga cctcggccag cggcagcgcc ggcaggccgg ggagctggac cttggcccgg
      781 acgaggttgg cgtggccctc ggtggtccgc ccgtcggcgg tggcccgggc ggtggcgacg
      841 tcggcccgca gcaggctgac cggccggccg ccgtcgatgc cgtccagccg gacggtgagc
      901 gcggtcttgt cggccgaggc cggggcgtgg acctcgttga ggacggcccg gacggggacg
      961 ttcaggctct tgtcgagcag gttgacgtcc agggcggtgc gcagcaccac cgcccccgag
     1021 gtgccgtggc tatgggcgtc gtcgccggtg gcgtacgcgg cgggcgcggc ggcgagggcg
     1081 acggcgggac cggccgtcag gacggcggcg gccagtacgg cgccgatccg cccggtggtg
     1141 ccggtggagc cggcgaatct ggtggaacgg gtggacacgt gtgtggaacc cccacatgag
     1201 agatggcgcc gccgaccgcg ccaatggggg acatcacgcg ggccgacggc atcgactcgc
     1261 acatcgtgta cgcacggagg gtgaacagtt agccacgcga ggttagttca ccccaatggg
     1321 tggttttcgc atccttattc gatttcctcc gacccctccg ccccggcggg cctcccccgc
     1381 cacagccgta ccgggccgcg ggggcccggc acggcccgcc gggcggggtg ccgaaggtcg
     1441 ctcagcccac gacccggccg cgcagcacca ccaggcgcgg cgccgccagc acccggacgt
     1501 cgactcgcgg gtcggtctcg tagacgacca ggtccgcgga ggcgccctcg gtgagcccgg
     1561 ggcggccgag ccactcccgg gcgccccagg tcgtcgccga gagggcggcc tcgacgggca
     1621 gccccgcctt catcaactcg gcgacctcgt cggcgaccag gccgtgcggc agcgagccgc
     1681 cggcgtcggt gccggcgaag atcgggacgc cggcatcgta ggcggcccgc acggtgtcgt
     1741 agcgccgggc gtgcagccgg cgcatgtggt ccgcccagcg cgggaacttc gtctcgccgc
     1801 cggccgcgag ctgcgggaag gtcgcgatgt tgaccagcgt ggggacgatc gcgacctggc
     1861 gctcggcgaa gagcgggatg gtgtcctcgg tcaggccggt ggcgtgctcg atgcagtcga
     1921 taccggcctc gaccagcggg gcgagggact cctcggcgaa gcagtgggcg gtgacccggg
     1981 cgccgagccg gtgcgcctcg gcgatggcgg cggccacctc accgggcggc cagcaggcgg
     2041 ccaggtcacc ggtggagcgg tcgatccagt cgccgacgag cttgacccag ccgtcgccgc
     2101 gccgggcctc ctgggcgacg taggcgacca ggtcggccgg ctcgatctcg tgcgcgtagt
     2161 tgcggatgta gcgccgggtg cgggcgatgt gccggccggc gcggatgatc cgcggcaggt
     2221 cggggcggtc gtcgatccag cgggtgtccg agggcgagcc ggcgtcccgg atcaggaggg
     2281 tgccggcgtc ccggtcggtg agcgcctgct tctcgctggt ggcggcgtcc accggtccgt
     2341 gcgcgtccag tccgacgtgg cagtgggcgt cgaccaggcc cggcagcacc cagccggtca
     2401 cggtcctggc atcgcgtgcc agggccggcc gctcgaaggt gacccggccg tcaatgaccc
     2461 acagctcatc gcgaacgtcg tccggcccga cgaggaccct ccccttgagg tgcagcaccg
     2521 caccttcgct catggacgtc atgcacgcac tgtatgcggg ggcccgagcc ggcggccacg
     2581 gtgtgcgaag ggcccgcacc ggccatgctc cgggcggttt tcgcaaaact ccgcagcgcg
     2641 cccgggacac atcgaagcgc ggggcttccc gcatctcacc ggaagcgctt ggcggaactt
     2701 ctcgcacgac ttttaaggaa gcgcccagga cgccttctac cggcgaattc cctattcctc
     2761 agaagagcag cttcccgtat tcttatgccc gctttcctca agctcaaacg ccaagatttc
     2821 gctagtaccg agtctcataa ttcaggcact tcgcgggagc gttacgaagc tgtgactgac
     2881 tacacagcgt gtccgtttcg ccccgaacta tcggaccaaa ccgtgacact ttgcaaccgg
     2941 gtcgcgcgct cgtgcgcccc cgcgtgataa cacagatcag gttctcacgt aacccctgcc
     3001 cgcgatgcaa tttgcttttc gcctcggtaa atttaatttc catgaccgcc gcacaagcag
     3061 accatgccgg tgcccgaagc gaaatcagag aaataccgga gggcttcaag ctcgacaccc
     3121 cccgggtgga ggacggggcc gcgatctggc gcatcgcccg cgactcgaag acgctggacc
     3181 tgaactcctc ctacagctac ctcctgtggt gccgcgactt cgccgccacc tccgccgtcg
     3241 cccgcgacgc gacgggcgag ccggccgcgt tcatcaccgg ctatctgcgc ccggaccgcc
     3301 cggacaccct cgtcgtctgg caggtcgccg tcgatgcggc gcaccgcggg cggggcctgg
     3361 ccgccgccct gctggacggg ctgaccaccc gtgccgtcga cgaactgggc gtccgccgcg
     3421 tggagaccac gatctcgccc ggcaacaccg cctccaaccg gctgttcgcc tccttcgccg
     3481 agcggcactc ggtgctgatc gagcgcgaga tgctcttcga cgcggcgctc ttccccgagc
     3541 agggccacga ggcggaggtc ctgcacctca taggcccgtt cgaggcgccg cccggcagca
     3601 gacgctgacc ctggccgcgc gcacggcgcc ccccccggac ttctccctcc ccacactcat
     3661 ctcccaggag catgctgtga ccctcaccca gcccgatctg agcgtcttcg aaaccgtcga
     3721 gtcggaggtg cgcagctact gccgtggctg gcccaccgtc ttcgaccgcg cccagggcag
     3781 ccgcctgacc gacgaggacg gccacaccta cctggacttc ttcgccggtg ccggttcgct
     3841 caactacggg cacaacaacg cggtcctcaa acgcgccctg ctcgactaca tcgagcggga
     3901 cggcatcacc cacggcctgg acatgtccac cacggccaag cgggccttcc tggagtcgtt
     3961 ccagaacatc atcctgcggc cgcgcgacct gccctacaag gtcatgttcc cgggcccgac
     4021 gggcgccaac tccgtcgagg ccgcgctcaa gctggcccgg aaggtcaaga gccgcgagtc
     4081 gatcgtgtcc ttcaccaacg ccttccacgg catgtcgctg ggcgccctcg cggtcaccgg
     4141 caactccatg aagcgggccg gcgccggcat cccgctggtg cacggcaccc cgatgccgtt
     4201 cgacaactac ctcgacggcc agacgccgga cttcctgtgg ttcgagcggc tgctggagga
     4261 ccagggctcg ggcctgaaca agcccgcggc cgtgatcgtg gagaccgtgc agggcgaggg
     4321 cgggatcaac gtcgcccgct ccgagtggct gcgcgccctg gccgccctgt gccagcgcca
     4381 ggacatgctg ctgatcgtcg acgacatcca gatgggctgc ggccgtacgg gtgccttctt
     4441 ctccttcgag gaggcgggca tcgtgccgga catcgtcacc gtctccaagt ccatcagcgg
     4501 ctacggactg ccgatggcgc tcaccctgtt caagccggag ctggacatct gggagcccgg
     4561 cgagcacaac ggcaccttcc gcggcaacaa cccggcgttc gtcaccgcgg ccgccgccct
     4621 ggacacgtac tgggccgacg gccagatgga gaagcagacg ctggcccgcg gtgagatcgt
     4681 cgaggcgcac ctgaaggcca tcgccgagga gcacccgggc accttcgccg agtaccgcgg
     4741 ccgcggtctg gtgtggggcc tggagtgcac cgacaagagc ctcgccaaca agatcgcggg
     4801 gcgcgccttc gagctgggtc tgctgatcga gacctccggc ccgcagagcg aggtcgtcaa
     4861 actgctgccc gcactgacga ccacccccga ggaactggac gagggtctgc ggatcctcgc
     4921 ccgcgcggtc cgcgactgcg cctgaccgca gccctgcgcc gacatccctc gccgaaccac
     4981 agaaaggcat ccgacgcacc atgatcgtcc gctcgttcca ggagatcgaa ggcaccgacc
     5041 gccacgtcaa ggccaagtcc ggcacatggg agagcaagcg catcgtgctc gccaaggagc
     5101 gggtcggctt ctcgctccat gagacggtcc tctacgccgg caccgagacg tccatgtggt
     5161 acgccaacca catcgaggcc gtggtctgcg tcgagggcgt cgccgagctg accaacgacg
     5221 agaccggcga gaagcacctc atcacgcccg gcgtgatgta cctgctcgac gggcacgaga
     5281 agcacaccat gcggatcaag gaggacttcc gctgcctgtg cgtcttcaac cccccggtca
     5341 ccggtcgcga ggaccacgac gagaacggcg tctacccact gctcaccgag cccgaggcgg
     5401 gctgagctgc gccgcaccgc cgcgggccgc cgcgatgcca tgagattccg tacgagagga
     5461 gaggaaggca ccaccatgac caccgcaccc gagcgcaccg ccgacctgta cccgacccgt
     5521 gggacctccg aggtcatcac cccgcggaag gacccggtgg tgtggtcgcg gcccggagtc
     5581 gaggggccct tcgccgctgc cgagctgagc gacttcgacc gtgacggctt ctgcgcgctg
     5641 ccggagctgc tcaccgcgga cgaggtcgcg gtgtaccgcg ccgagctgga ccggctggtg
     5701 ggcgacccgg cgatgcgcgc cgacccgcgc tcgatcgtcg agccgacgtc gcaggccgtc
     5761 cgctcggtct tcgaagtcca ccggatcagc gaggtgttcg cgggactggt cgccgacccc
     5821 cgggtggccg gccgggcccg gcagatcctc ggctcggacg tctacgtcca tcagtcgcgg
     5881 attaacgtca agcccgggtt cggtgcctcc ggcttctact ggcactcgga cttcgagacc
     5941 tggcacgccg aggacgggct gccgcacatg cgcacggtgt cggtctcgat cgcgctgacc
     6001 gagaactacg acaccaacgg cggcctgatg atcatgcccg ggtcgcaccg gtacttcgtc
     6061 gggtgcgagg gcgccacccc gaaggacaac tacaagaagt cgctccagat gcaggacgcg
     6121 gggacgccgt cggacgaggc gctgacgacg atggcggacc ggcacggcat ccgcctgttc
     6181 accggccggg ccggctcggc gacctggttc gactgcaacg ccatgcacgg ctccggcgac
     6241 aacatcacgc cgtacccgcg cagcaacgtc ttcatcgtct tcaacagcgt ggagaacccc
     6301 gcggtggagc cgttcgcggc gccggtccgg cgcccggagt acatcggggc gcgcgccccg
     6361 ttcgttccgg tcacatagcg ggcgcccggt gctccggcac cgggcgggga agcggggagg
     6421 ccccgcgcgc ggccgccgca cggcaccact ggggtgcggt gcggcggtcg ccgcgcgcgg
     6481 ggcctcgacc gcgtcgcgac ggccgtgact gacgctccgt cagttctcgt cgtccagcgc
     6541 ggggtagtcc agatagccgc ggtcgtcgcc gccgtagaag ccgtcccggt ccggggtgtt
     6601 gaacgggccg ccggcggcca gccggcgggg caggtcgggg ttggcgatga acagcgcgcc
     6661 gtacgccagc agatcggcct cgccgcgctc gacgagcgag agctcctcgg ggccggtggg
     6721 ccggccttcg gtggccgggt tgagcaggaa cgggccgtcg aaccgcttcc gcagccgggc
     6781 cagcagctcc aggtcggtca cgtcgcaggt gtgcagatag gccagtccca gcggggcgac
     6841 ggcctcgatc agcgcggtgt aggtggcctc gggcgcgggc tcgtcgatgt cgttgaacgg
     6901 gttgccgggc gagagccgca gaccggtgcg gtgcgcgccg accgcgccgg cgaccgcctt
     6961 gaccacctcg atggcgaacc gggcgcgggc ctcgtcggag ccgccccacg cgtcggtgcg
     7021 ccggttggcg ttgggggcca ggaactggtg gatcaggtag ccgttggcac cgtggatctc
     7081 cacgccgtcg aagccggcct cgatggcgtt gcgggccgcg gcggcgaaac cgtcgacggt
     7141 cgcgcggatc tcctcgtcgg tcagctcgcg cggggtgacg aaatccgtcg ggccgttctg
     7201 ggtgaagccc tggcccgccg ccttcaccgc ggacgggccg accggctcca gcccgtcggg
     7261 cagcagcacc gggtggccga tccgaccgcc gtgccacagc tgggcgaaaa tccggccgcc
     7321 ggccgcgtgg acggagtcgg tgacccggcg ccagcccgcg acctggtccg cggtgtgcag
     7381 ccccggggta agcgggtagc cgcggccgac cacctcggtc tgggtggcct cggtgatgat
     7441 caggccggcc gaggcccgct gggtgtagta cgcgaccatg gacggggtgg ggacaccctc
     7501 ggcggtggcg cggttacggg tcatcggcgc catgacgatg cggttggcga gcggtatacc
     7561 ggcgagctct atggggtcga acgcagtggt cactgacggc ctttcagcgg tttgtcatgg
     7621 ttcgtcaact cggctgtcga accgaacatt ggccattacc tcagcggtcg atccccgccc
     7681 catattccct gggccgtatt cccctggggc cccgaaccgc tcgtcaagga accaacaggg
     7741 acagcgcccg gctcacgtcc tcggtcgagt tgtagaggtg gaacgccgcc cggagcagcc
     7801 cgccgcggac cgagacccgc accccggccc gggccagccg cggttcggcg tccgccagcc
     7861 ccggggtgga gacgatcgcg gacccgggcg cggcccgcgg ggtgaagccg gcggcctcca
     7921 gcccggcccg gtactgctgc gccagcgcga cgttgtgggc gtggacggtg tccacgccga
     7981 gctccgcgag cagcccgagg gagtgctcgg cggccacgta ggagtagtgg gcgtgcggct
     8041 cgtcgtagcg gcgggcggtc gccgccggtc ggtcgaccgg accgtagttg gactcccccg
     8101 ggtcctcccc ggcgacccac cccgcgtgcg ccggcaccgg ccaccccggg ccgcccggct
     8161 ccccccgctc cccgccgaag accatgaacg tcgtcccgcg cgggcacagc agccacttga
     8221 acgccccgca caccacgtag tcgaagtccg cgagccgcag cggcagccag cccgccgcct
     8281 gtgtgatgtc gacatacgtc agcgccccgt gcgcccgggc ggcgtcccgg atggcggcca
     8341 ggtcggcgat ccggccgtcc agcgcgtgca ccgcgctcac cgcgaccagc gccgtcccgg
     8401 gccgcaccgc gtcggcgagc gcctcccacg gcaccgcccg caccgtgcac ccggcgcgcg
     8461 ccgccagcgg gttcaccagg gagctgaagt ccccctcggc gaccagcact tcgctgccgg
     8521 ccggcagcga gcccgcgacg aaagccgact gcaccgcgac cgaactgccc agcgccaccc
     8581 gctcctcggg cacccccatc agtcgggcga aggcgccgcg cacccgggcg cccgtcccga
     8641 ggtagtcgcg ccccatcgtc ccgtgcgacg ccgactccgc cacggcggcg gtcagcgcgg
     8701 cggcgctccg cgcgggcagc agcccgcagg acgccgagtt cagatagacc gtctcgggcg
     8761 caaactcgtc gccgcccagt gccatgtgtc gctgcatggc cccactctgc tcggtgaccg
     8821 ccccggcgtc cacgggaatg cccgggccgc gacgccgggg cggaccggcc ggtgggtcag
     8881 accgcgcagc tcccgtcgcc gcagctgtcc gcgtcgtcgg cggccagcac ctggacggcg
     8941 gagtcggcgt gcgcccgctc cagggcctgg cggaagacgt cggcgggctg ggcgccggag
     9001 acgccgtagc ggcggtcgat gacgaagaag gggacgccgg tggcgcccag ctcggcggcg
     9061 gcgcgctcgt cggcacggac ctcgtcggcg taggcgtcgg ggtcggcgag gacccgcgcg
     9121 gcctcggcct cgggcacgcc ggccccgacc gcgatcccgg tcagcaccgc ggggtccgcg
     9181 agctgccggg cctcggcgaa gttggcgtgg tagagggcgg tgagcagcgc gtcctggacg
     9241 ccgtgcgcct tggccaggtg gagcagccgg tgcaggtcga aggtgttgcc gtggatgcgg
     9301 tcggagcggt agcccagccc ctcgccggcc gccgcctcgg ccacccgggc ctccatcgcc
     9361 tcggcctgtt cccggctcac gccgtacttc gtcgcgagca tgtccaggat cgggacgtcg
     9421 gtggccggcg gggcggccgg gtccagttcg aaggaacggt ggacgacctc gacgtccgcc
     9481 cgctgggcga aggccgccag accggcctcg aaccgggcct tgccgatgta gcaccagggg
     9541 caggcgatgt ccgaccagat ttcgacgcgc atggggatga ctctcgatga ctttcctcgg
     9601 ggggaccggc ccgcggccga tcaggggtac gaaggggtgc aacggtgccg gcggcgggct
     9661 tattccggat gcccccgggc gtcccgaacg ggtcggccgc cgcgcgatgc gtcggatgcg
     9721 cgtcggccgg gcggtaccgt cccgctatgg gcacgccacc gccgcacgcg accgacgggt
     9781 tctgggagtc gaccggggcc accaagacgt tcacgcatcc gctcgacgcc gagctgctga
     9841 cggagttcgt gccgcggacc gcgcgggtgc tggactacgg ctgcgggtac ggccggctca
     9901 ccgccgaact ggtcgggtcg gggtaccggg cggtgcgcgg ggtggaccgg tccgcggcgc
     9961 tgatcgcgcg ggggcggcgg gagcatccgg ggctggccct gatgcgctgg gccgggttcc
    10021 cgctgccgtt cgcggaccgc agcttcgacg cggcgctgct gttcgcggtg ctgacctgtg
    10081 tgccggacga cgccggccag cgggcgatcg tcggggagct ggggcggctg gtgcggcccg
    10141 gtggggtgct ctacgtgagc gatgtgccgc tgcaggacga cgcgttccac cgcgggcagt
    10201 acgagcggtt cgcggcgcgg tacgggacgt acggggtgtt cgagacggag gacggcgggg
    10261 tgttccggca ccatccgccg gagtggctgc gggggctgct gcgggaggcg ggattcgcgg
    10321 tgctgcggga gcgccgcacg gtggtgccga cgctggacgg ccgcaccgcc gagcggctgc
    10381 agatcatggc ccggcgggag ccggc
//
