LOCUS       NZ_LJSN01000002        10216 bp    DNA     linear   CON 29-MAR-2024
DEFINITION  Streptomyces noursei strain JCM 4701 scaffold2, whole genome shotgun
            sequence.
ACCESSION   NZ_LJSN01000002
VERSION     NZ_LJSN01000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces noursei
  ORGANISM  Streptomyces noursei
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 4596274)
  AUTHORS   Graham,D.E., Mahan,K.M., Klingeman,D.M., Fida,T., Giannone,R.J.,
            Hettich,R.L., Parry,R.J. and Spain,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-SEP-2015) Biosciences Division, Oak Ridge National
            Laboratory, PO Box 2008, MS-6038, Oak Ridge, TN 37831-6038, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2015
            Assembly Method :: SPAdes v. 3.6; SMRT Analysis v. 2.3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300.0x
            Sequencing Technology             :: Illumina MiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002891315.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 20:38:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,947
            CDSs (total)                      :: 8,851
            Genes (coding)                    :: 8,489
            CDSs (with protein)               :: 8,489
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 362
            CDSs (without protein)            :: 362
            Pseudo Genes (ambiguous residues) :: 0 of 362
            Pseudo Genes (frameshifted)       :: 118 of 362
            Pseudo Genes (incomplete)         :: 301 of 362
            Pseudo Genes (internal stop)      :: 23 of 362
            Pseudo Genes (multiple problems)  :: 74 of 362
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:29:39
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2014505
            Orig. end                         :: 2024721
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LJSN01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10216
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="12"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10216
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10216
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5216](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5216)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(726..740)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 17.3"
                     /tool="antismash"
     gene            complement(1687..3966)
                     /locus_tag="AOB60_RS10380"
                     /old_locus_tag="AOB60_09745"
     CDS             complement(1687..3966)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018540973.1"
                     /locus_tag="AOB60_RS10380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOB60_09745"
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="WP_073448127.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 1.5e-38, bitscore:
                     129.5, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAPEAAAAPPQHLPPAGRGRHRPEGAPPTRRRRPPAAAPKAKKS
                     VRTPTVLQMEAVECGAASLAMVLAHYGRHVPLEELRIACGVSRDGSRASNLLKAARSYG
                     LSAKGMQMEPAALAEVQAPAILFWEFNHYVVYDGMGRRLGRRGVHINDPDKGRRFVPTE
                     DFDTSFTGVALVFEPTEAFRKGGRRPSVRSALPARLRGTAGTLLAALLASLLLVGVGAA
                     LPALSRTYIDMFLIGNQTSLLGPLFAAMSAMVVLTAVLTGLQQANLLRGRIISSTLSSA
                     RFLRHLLRLPVTFFSQRSPADLVQRLQSNDAVAETLARDLAAAAVDGVVVVLYAVLLWT
                     YDPQLTVIGVLIALLNVVAMRIVVRLRATHTQKLRADTARLTNTSYTGLQLIETMKATG
                     GENGYFRRWAGQHATTLEEQQRLGVPSAWLAVVAPTLATLNSALILWIGGLRAVEGHIS
                     IGLLVAFQALVTRFTAPITRLNGVAGRIQDFAADVARLKDVESFPADAHYSRPDADAGT
                     RRLKGHITLDGVTFGYSPLDAPLLTGFSLAVGPGRQVALVGGSGSGKSTVSRLISGLYT
                     PWEGTIRIDGQRLADIPRSALAASVSFVDQDVFLFEGTVRDNVALWDPSIPDDAVIAAL
                     QDAALYDDVIARRPDGIHSRVEQDGRNFSGGQRQRLEIARALVRRPSILVLDEVTSALD
                     AGTEAVIMDNLRRRGCACVIIAHRLSTVRDSDEIVVLDHGTVVERGRHEELVAAGGAYA
                     ELVKEH"
     gene            complement(3963..4769)
                     /locus_tag="AOB60_RS10385"
                     /old_locus_tag="AOB60_09750"
     CDS             complement(3963..4769)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018540972.1"
                     /locus_tag="AOB60_RS10385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOB60_09750"
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_073448128.1"
                     /transl_table=11
                     /translation="MQFRQQALSKLQSPEELDLPVRFARPQGWLVLTVTVLVMIAATVW
                     AVTGTVSATLTAPGILTHGRGSYVLQSPVAGQVTAVLAEEGQPVAAGAPLARIRTVQGT
                     TTVVRALAAGRLTSLVATIGAVVTTGADLAAVERTNGPHDPLTATLYVPAASAATLPAG
                     AAVDLTVASAPTQRFGVLRGHVAAIGRTAQSRQRISAFLGSSQLGEQFSRAGQPVAVLV
                     RLDTASTATGYAWSTSDGPPFALDSMTQATAAVHLAAQHPIDWLLS"
     misc_feature    4734..4746
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 16.37"
                     /tool="antismash"
     gene            complement(5001..5216)
                     /locus_tag="AOB60_RS10390"
                     /old_locus_tag="AOB60_09755"
     CDS             complement(5001..5216)
                     /GO_component="GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016576071.1"
                     /locus_tag="AOB60_RS10390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOB60_09755"
                     /product="type A2 lanthipeptide"
                     /protein_id="WP_073448129.1"
                     /sec_met_domain="strepbact (E-value: 7.8e-22, bitscore:
                     75.5, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNAQVQTQEISDADLDNVSGGLVGGVLSTVTTTVGSIAPVGSVL
                     ATATGLVQQTAGLDVNGIVGGATAGL"
     misc_feature    complement(5234..5248)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.56"
                     /tool="antismash"
     misc_feature    5235..5248
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.98"
                     /tool="antismash"
     misc_feature    complement(5235..5249)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.62"
                     /tool="antismash"
     misc_feature    5236..5249
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.16"
                     /tool="antismash"
     misc_feature    complement(5425..5440)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 16.99"
                     /tool="antismash"
     misc_feature    complement(5588..5606)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 18.26"
                     /tool="antismash"
     misc_feature    5690..5707
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.91"
                     /tool="antismash"
     misc_feature    5699..5715
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 15.68"
                     /tool="antismash"
     misc_feature    complement(5709..5723)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.67"
                     /tool="antismash"
     misc_feature    complement(5710..5725)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.04"
                     /tool="antismash"
     gene            5893..6933
                     /locus_tag="AOB60_RS10395"
                     /old_locus_tag="AOB60_09760"
     CDS             5893..6933
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018540970.1"
                     /locus_tag="AOB60_RS10395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOB60_09760"
                     /product="PHP domain-containing protein"
                     /protein_id="WP_102924522.1"
                     /transl_table=11
                     /translation="MDPVAALERIAFLLERQGAVTYRVRAFRTAASVLDGLATEELRER
                     AGNGTLGRLKGLGPKTTRVVEEALAGQQPAYLTRLTEEAGGPLVEGERGLALRRALRGD
                     CHLHSDWSDGGSPVEAMARAARDLGHDWCALTDHSPRLTVAHGLTAERLRRQLDVVAEV
                     NARLAPFRLLTGIECDILDDGSLDQEPELLDRLDVVVASVHSKLRMDRRPMTRRMLAAV
                     GNPLVDVLGHCTGRQLTGRLRPESAFDAAEVFAACAEHRTAVEINCRPDRFDPPRRLLR
                     QALDAGTLFAVDSDAHAPGQLDWQIYGCARAEECGVPADRIITTWTARQLTTWTRTGRT
                     PHGAGG"
     gene            complement(6976..7884)
                     /locus_tag="AOB60_RS10400"
                     /old_locus_tag="AOB60_09765"
     CDS             complement(6976..7884)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091736.1"
                     /locus_tag="AOB60_RS10400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOB60_09765"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_039638460.1"
                     /transl_table=11
                     /translation="MIEARRLHILRALADHRTVTAAAAALYLTPSAVSQQLAALEQETG
                     HRLVERGARGVRLTAAGDILLGHANAVLAQLERAEAELAAYDKGEAGTVTVAAFATGIA
                     LVLAPALAELAGSAPGIRVRVQDAEGDASLLMVLDRQVDVAVAVEYRGAPDADDPRLTR
                     VPLYAEPFDAVLPPGHPLAGAAAVPLAGLAKDPWIGPYSGNPCHDVVVLACEYAGFRPR
                     LTHSSDDFRAVVALASAGVGVALVPRSALRGMDLGGVVVRPVDGAPPTRRVFAAVRRGA
                     EDHPLFRPVLAALRTAASAQG"
     gene            complement(7936..9168)
                     /locus_tag="AOB60_RS10405"
                     /old_locus_tag="AOB60_09770"
     CDS             complement(7936..9168)
                     /EC_number="2.3.1.29"
                     /GO_function="GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA]"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (58-401). E-value:
                     1.9e-71. Score: 233.0. Matches aSDomain:
                     nrpspksdomains_AOB60_RS10405_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1109: 8-amino-7-oxononanoate synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012999157.1"
                     /locus_tag="AOB60_RS10405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOB60_09770"
                     /product="glycine C-acetyltransferase"
                     /protein_id="WP_102923462.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 1.4e-69, bitscore:
                     232.9, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFDAVRDDLRTALDEIRAAGLHKPERVIGTPQSATVKVTAMGSPL
                     LEGSRELGEGRPGEVLNFCANNYLGLADHPEVIAAAHGALDRWGYGLASVRFICGTQEV
                     HKELERRISGFLGQEDTILYSSCFDANGGVFETLLGPEDAVISDALNHASIIDGIRLSK
                     ARRLRYANRDMDDLERQLKDAEGARRTLIVTDGVFSMDGYLAPLREICDLADRYGAMVM
                     VDDSHAVGFVGPGGRGTPELCGVMDRVDIITGTLGKALGGASGGYVAARAEIVALLRQR
                     SRPYLFSNSLAPVIAAASLKVLDLLESAGDLRERLNANTALFRSRMTEEGFAILPGEHP
                     IAPVMIGDAAEAARMAELLLERGVYVIGFSYPVVPKGEARIRVQLSAAHSTEDVHRAVD
                     AFVAARAALAG"
     aSDomain        complement(7966..8994)
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AOB60_RS10405_Aminotran_1_2.1"
                     /evalue="1.90E-71"
                     /label="AOB60_RS10405_Aminotran_1_2.1"
                     /locus_tag="AOB60_RS10405"
                     /protein_end="401"
                     /protein_start="58"
                     /score="233.0"
                     /tool="antismash"
                     /translation="EVLNFCANNYLGLADHPEVIAAAHGALDRWGYGLASVRFICGTQE
                     VHKELERRISGFLGQEDTILYSSCFDANGGVFETLLGPEDAVISDALNHASIIDGIRLS
                     KARRLRYANRDMDDLERQLKDAEGARRTLIVTDGVFSMDGYLAPLREICDLADRYGAMV
                     MVDDSHAVGFVGPGGRGTPELCGVMDRVDIITGTLGKALGGASGGYVAARAEIVALLRQ
                     RSRPYLFSNSLAPVIAAASLKVLDLLESAGDLRERLNANTALFRSRMTEEGFAILPGEH
                     PIAPVMIGDAAEAARMAELLLERGVYVIGFSYPVVPKGEARIRVQLSAAHSTEDVHRAV
                     DAF"
     misc_feature    complement(9367..9376)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     misc_feature    9432..9445
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.47"
                     /tool="antismash"
     misc_feature    complement(10034..10048)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: medium, score: 18.79"
                     /tool="antismash"
ORIGIN
        1 agcgccagcg gcacgctgta gaggagcagc atcacgaggt tgaccgcgcc gaccgtggcg
       61 gcctgcacgg ccaccggccc gacgccggcc agcacctggc ggatggcgct gatgcccatg
      121 gcggcactgg ccagttcacc ggtggagcgg ccggcgaaga acttcgtcgg caggcgcagc
      181 agccggtccc agacagccgg ctggaggacg ctctcgatcc ggccctcgat gcgcagcacg
      241 gtgaggttct gcagcagggt gaaggccgcc gcgaccacgc cggcgatgat gacggcgagc
      301 gcgacctgga cgatcgggcc gcgggcggcg gccggtacgt agacgccgag gaccttgccg
      361 gtggcgagcg ggacgagggc gccgagggcg accgtcacca gtccgctcag ggcgaggttg
      421 cgcaggtcgg cgcggttccc gcggaggctg aagcgcatca gccgccacag cgtgagccgg
      481 cgttcgggca ggggccggta gaacatcacc gcgtgcggct cgaactcgtc ggcggtgtcc
      541 ttgccgatcc ggatccgcag cccggaggcg gggttgaccg cctcgtaccg gccgcgccgc
      601 cacaacagcg ccaccggggc accggacttg gcgcggtggc ccaccagggg cccggcgtcg
      661 gtgcgccacc agcggccgtc gaggcggacc gcgcgggtgc ggatccggga ggcgagggcg
      721 atccgttcga cggggctgat ccggtcgccg gcggcggcgc ccttgggcgg ttcggtgagc
      781 acgatgccgg cggccccggc gaccttccgg cagaccgcga aggtcaggtc gtcgccggcc
      841 cggtcgcggc ccgcggcggc gcgtccggag cggtccttgc cgatggacgc cagcagtgcc
      901 tggtcggcgc gttcccggac ggcctcgccg gctttgatgc cggccgccgc gcggtcctcg
      961 tgagcccgct ccaactgctc gatccagcgg tcgacggcgg acagcagccg gtactgctgg
     1021 ttgaccatcc gctgccacag cgccgcgtcg acaagcaggt cggcggcggc ctcggcgctg
     1081 taggaggcgc cgtactggac gctgccgggc ggcaccggca tccacaggac gtcgtcgtcg
     1141 gcgaccgctt cgtcgctggg ccggccgtcc aacggcgcct cgaacagcac gcccagaccg
     1201 cgcgcggtgc cgagcgcgaa ggcgtgctcc aggggggtgg ccggctgccc gccgtaggtg
     1261 tcgtcgggcc cgaaggtgcc ttcgtgccca tacgcgccct ggtcgccgtg gccgccgtgg
     1321 tcggcgcggg gcagttctcg cagggcgatc cggcgcagtc cgcagtgctg ggaggggcgg
     1381 ccgacgaggg tgtgctgggg gccttggacg ggaccgagca gcaaggtgcc ggcttcgagg
     1441 cggccgagga agtgccagtg gccttcctga ccggcgtcca ccgcgaacag gtcgagggtg
     1501 ccgccggtga ccagccacag cacgtgcggg ccttcgaggg ggacgtcacg cagcccggcg
     1561 cagtcgacgg gggtgccgag gccgcccagg gcgtgcgtga ccgtgtcgac gccgtgcgga
     1621 tccgcggccg gtgcggccgg ctgcgtggcg gatcccgcgt ccggggacgg gtgtacggat
     1681 gccacgtcag tgctccttga ccagctcggc gtaggcgccc ccggcggcga ccagctcctc
     1741 gtgccggccg cgttcgacga ccgtgccgtg gtcgagcacc acgatctcgt cgctgtcgcg
     1801 gacggtgctc agccggtggg cgatgatgac gcaggcgcag ccgcggcgcc gcaggttgtc
     1861 catgatgacc gcctcggtcc cggcgtccag ggcgctggtg acctcgtcga ggacgaggat
     1921 gctggggcgt cggaccagcg cccgggcgat ctccaggcgc tgccgctggc cgccggagaa
     1981 gttgcggccg tcctgttcga cgcggctgtg gatgccgtcg gggcggcggg cgatgacatc
     2041 gtcgtagagg gcggcgtcct ggagggcggc gatcaccgcg tcgtccggga tggaggggtc
     2101 ccacagcgcg acgttgtccc ggacggtgcc ctcgaagagg aagacgtcct ggtcgacgaa
     2161 ggagaccgag gcggccagcg cgctgcgcgg gatgtccgcg aggcgctgtc cgtcgatccg
     2221 gatcgtgccc tcccaggggg tgtagaggcc ggagatcagc cgagagacgg tggacttgcc
     2281 gctgcccgag ccgccgacca gggcgacctg gcggccgggg ccgacggcca aggagaagcc
     2341 ggtcagcagc ggggcgtcga gcgggctata gccgaaggtg acgccgtcga gggtgatgtg
     2401 gcccttaagg cggcgggtgc cggcgtccgc gtccgggcgg gagtagtggg cgtcggcggg
     2461 gaagctctcg acgtccttga ggcgggccac gtcggcggcg aagtcctgta tgcggccggc
     2521 gacgccgttg agccgagtga tcggtgcggt gaagcgggtc accagggcct ggaaggcgac
     2581 cagcagaccg atggagatgt ggccctcgac ggcgcgcagg ccgccgatcc acaggatcag
     2641 tgcgctgttg agggtggcga gggtgggggc gacgaccgcc agccaggcac tggggacgcc
     2701 gagccgctgc tgctcctcca gcgtggtggc gtgctggccg gcccagcggc ggaagtagcc
     2761 gttctcgcca ccggtggcct tcatggtctc gatgagttgg aggccggtgt aggaggtgtt
     2821 ggtcaggcgg gcggtgtcgg cgcgcagctt ctgggtgtgg gtggcgcgca gccggaccac
     2881 gatccgcatg gcgacgacgt tgagcagcgc gatcagcacg ccgatgacgg tgagctgggg
     2941 gtcgtaggtc cacagcaaca ccgcgtagag gacgaccacc acgccgtcga cggcggccgc
     3001 ggccaggtcg cgggcgaggg tctcggcgac cgcgtcgttg gactgcaggc gctggacgag
     3061 gtcggcgggc gagcgctggg agaagaaggt gaccggcagc cgcagcagat ggcgcaggaa
     3121 gcgggcgctg ctcagggtgg aggagatgat ccggccgcgg agcaggttgg cctgctggag
     3181 gccggtcagg acggcggtga gcaccaccat cgcgctcatc gcggcgaaca gcggccccag
     3241 cagcgaggtc tggttgccga tcaggaacat gtcgatgtag gtgcggctga gcgcgggcag
     3301 cgcggcgccg acgccgacca gcagcaggct ggcgagcagc gccgccaaca gggtgccggc
     3361 ggtgccgcgc agccgggccg gcagggcgct gcgcacgctg ggccggcggc cgcccttgcg
     3421 gaacgcctcg gtgggctcga agaccagggc gacgccggtg aagctggtgt cgaagtcctc
     3481 cgtcggcacg aagcggcggc ccttgtcggg gtcgttgatg tggacgcccc ggcggccgag
     3541 gcggcggccc atgccgtcgt agacgacgta gtggttgaac tcccagaaga ggatggccgg
     3601 tgcctggacc tcggcgaggg cggccggctc catctgcatg cccttggccg acaggccgta
     3661 actgcgggcg gccttgagga ggttgctggc ccgggagccg tcgcgggaga cgccgcaggc
     3721 gatccgcagt tcctccagtg gcacgtggcg tccgtagtgg gcgagcacca tggccaggga
     3781 ggcggcgccg cactccaccg cttccatctg gagcacggtg ggggtgcgca cggacttctt
     3841 cgccttgggg gcggcggccg gcggtctgcg gcggcgggtg ggtggcgcgc cctcggggcg
     3901 gtgccgtccg cggccggcgg gcgggaggtg ctgcgggggt gccgcggcgg cttcgggtgc
     3961 ggtcacgaga gcagccaatc gatcgggtgc tgcgcggcca ggtggacggc ggcggtggcc
     4021 tgggtcatcg agtccagggc gaacggcggg ccgtcggagg tggaccaggc atagccggtg
     4081 gcggtggacg cggtgtcgag ccggaccagc accgcgaccg gctggccggc gcgggagaac
     4141 tgctcgccga gctggctgct gccgaggaac gcggagatcc gctggcggct ctgggcggtg
     4201 cggccgatgg ccgcgacgtg gccgcgcagc acgccgaacc gctgggtggg ggcggaggcg
     4261 acggtgaggt ccacagcggc gccggcgggg agggtggcgg cgctcgcggc cgggacgtag
     4321 agggtggccg tcagggggtc gtgggggccg ttggtccgct ccaccgcggc gaggtcggcg
     4381 ccggtggtga cgaccgcgcc gatggtggcg accaggctgg tcagccggcc ggcggcgagc
     4441 gcgcggacga cggtggtggt gccttgtacg gtccgtattc gcgccaacgg ggcgccggcg
     4501 gcgaccggtt gcccctcctc ggcgagaacc gcggtgacct ggccggcgac cgggctctgc
     4561 aggacgtagc tgccgcggcc gtgggtgagg atgccggggg cggtgagggt ggcggagacg
     4621 gtgccggtga ccgcccagac ggtcgcggcg atcatgacga ggacggtgac ggtcagcacc
     4681 agccagccct gggggcgggc gaagcgcacc ggcaggtcga gctcctcggg cgattgcagc
     4741 ttggaaaggg cctgttggcg gaactgcacg acactcttcc ctcaactgca ggggctggta
     4801 cgggagtagc gggagtgtac cgccgccgga gatccggtgg cgggttcccg gcggcgaaca
     4861 gccgggtgcc ccggacaacc cggccggaac cgggttgtcc ggggcactcg gtacggcaga
     4921 tcggcaggga gggggcgccg actgccgtca cgaccgttcg tcgccgaccg tgccgcgcgg
     4981 cggcacgggg gcggctcgga tcagagaccg gcggtcgcgc cgcccacgat gccgttgacg
     5041 tcgagaccgg cggtctgctg gaccaggccg gtggcggtgg cgaggacgct gccgaccggg
     5101 gcgatcgagc ccacggtggt ggtgacggtg ctcagcacgc cgccgacgag gccaccggac
     5161 acgttgtcca ggtcggcgtc ggagatttcc tgggtctgca cctgggcgtt gttcatgatg
     5221 tttcccttcg gatcaatatc aagaaaaaac aagcgaaacg ggccgtccgg tgggtgaccc
     5281 ggccgcggcc acggcagtcc ggcccccgat atcgggaaac cccggcgact tccgcggtgc
     5341 gatggatcaa agcatgcggg cgggtttccc tgctaatcgg atgagcagcg atccggacgc
     5401 gaccacgccg cgaaattacc ggacgtgtac gtgtgtccat ggcttgatcg aagcatcttc
     5461 acatctcctc cggtttcttt tccgccgaag ggtcgttgcg attcccgcgg aatcgggcat
     5521 tccggaacta gaccgtcgcc caccagggcg gccgtgtcac ccgaaacccg gaagcgtgtg
     5581 ccggctgcgc attcggtgtg cagatttcct ggaggtcgcc gaacttaacg tccggctgac
     5641 gcgccggccc gccgagaagt ccgattcgcg tttccgcagt acggccggga tgttgcccag
     5701 gttttaaccg aacatgattg cccttgactt ccggtgcact aatgaccgga atcatcacgg
     5761 ctatcacccc gtcattccga tggaaacgga tcgagcatcc agagagcgga catattcgca
     5821 taactgcggt aggaggcatt cgccggcccg accgcggtgg cagccgcgcc ccgcccggtg
     5881 cacggtgggg aggtggatcc cgtggcggcg ctggagcgga tcgcgttcct gctggagcgg
     5941 cagggggcgg tgacgtaccg ggtacgggcg ttccgcacgg cggcgtcggt gctcgacgga
     6001 ctggccacgg aggaactgcg cgagcgcgcc gggaacggga cgctggggcg tctgaaggga
     6061 ctcggcccca agacgacccg ggtcgtcgag gaggcactgg ccgggcagca acccgcctat
     6121 ctgacgcggc tgacggagga ggccggcggc ccgctggtcg agggggaacg agggctggcg
     6181 ctgcggcggg ccctgcgcgg cgactgccat ctgcactcag actggtcgga cggcggcagc
     6241 cccgtcgagg cgatggccag ggccgcccgc gacctcggcc acgactggtg cgcactgacc
     6301 gaccactccc cccggctcac cgtcgcccac gggctgaccg ccgagcggtt gcggcggcag
     6361 ctggacgtgg tcgccgaggt caacgcgcgc ctggcgccgt tccgactgct gaccgggatc
     6421 gagtgcgaca tcctcgacga cggctcactg gaccaggagc cggagctgct ggaccggctc
     6481 gacgtcgtgg tcgcctcggt ccactccaaa ctgcggatgg accggcggcc gatgacccgg
     6541 cggatgctgg ccgcggtcgg caatccgctg gtggacgtcc tcggccactg caccggccga
     6601 cagctcaccg gccggctccg cccggagtcg gcgttcgacg ccgcggaggt cttcgcggcc
     6661 tgcgccgagc accgcaccgc ggtggagatc aactgccgcc cggaccgctt cgacccgccg
     6721 cgccgcctgc tgcggcaggc cctggacgcc ggcaccctct tcgccgtgga cagcgacgcg
     6781 cacgcgcccg gccagctgga ctggcagatc tacggctgcg cccgcgccga ggagtgcggc
     6841 gtccccgccg accggatcat caccacctgg acggcccgcc agctgacgac ctggacgcgg
     6901 accggccgca ccccgcacgg ggccggcggc tgaccgtccg ggacccgtcc gggacctggt
     6961 ccgggaccac tcgcctcagc cctgcgcgga cgccgccgtg cgcagcgccg ccaacaccgg
     7021 gcggaacagc ggatggtcct ccgcgccgcg gcggaccgcg gcgaagaccc ggcgggtggg
     7081 cggggcgccg tcgaccggtc gcaccaccac acccccgagg tccatcccgc gcagcgccga
     7141 gcgcggtacc agggccaccc cgacgcccgc cgaggccagc gccaccaccg cccggaagtc
     7201 gtccgaggag tgcgtcaggc gcggccggaa accggcgtac tcgcaggcca gcacgaccac
     7261 gtcatggcag ggattgccgg aatagggccc gatccagggg tccttggcga gcccggccag
     7321 cggcaccgcc gccgccccgg ccagcggatg cccgggcggc agtaccgcgt cgaacggctc
     7381 ggcgtagagc gggacccggg tcagccgggg gtcgtccgcg tccggcgcgc cccggtactc
     7441 gacggccacc gccacgtcga cctgtcggtc cagcaccatc agcaggctgg cgtcgccctc
     7501 cgcgtcctgg acccggaccc ggatgccggg cgcggaaccg gcgagttcgg ccagggccgg
     7561 ggcgagcacc agcgcgatgc cggtcgcgaa cgccgcgacg gtcaccgtgc cggcctcccc
     7621 cttgtcgtac gccgccagct ctgcctcggc gcgctccagc tgggccagca cggcgttggc
     7681 gtgaccgagc aggatgtcgc ccgcggcggt cagccggacg ccgcgggcgc cgcgctcgac
     7741 gaggcggtgg ccggtctcct gctccagggc ggccagttgc tgggagaccg ccgagggcgt
     7801 gaggtagagc gcggcggccg ccgccgtcac cgtgcgatgg tcggccagcg cacgcaggat
     7861 gtgcagccga cgagcttcga tcatggttcc agtttcgcag ggttcggcgg ggttctgtgc
     7921 cccggaaccc cgcggtcagc ccgccagggc ggcgcgggcg gccacgaagg cgtccaccgc
     7981 gcggtgcacg tcctcggtgg agtgggccgc ggacagctgg acgcggatcc gggcctcccc
     8041 cttggggacc accgggtacg agaagccgat gacgtacacc ccgcgctcca gcagcagctc
     8101 ggccatgcgg gccgcctcgg ccgcgtcgcc gatcatcacc ggcgcgatcg ggtgctcgcc
     8161 gggcaggatc gcaaagccct cctcggtcat ccgggagcgg aacagcgcgg tgttggcgtt
     8221 gaggcgttcg cgcaggtcac cggcggactc caggaggtcg agcaccttca gcgaggcggc
     8281 cgcgatcacc ggcgcgagcg agttggagaa caggtagggg cgggagcgct ggcggagcag
     8341 ggcgacgatc tcggcacgcg ccgcgacata gccgccggag gccccgccca gcgccttgcc
     8401 gagggtgccg gtgatgatgt cgacgcggtc catcacgccg cacagctcgg gggtgccgcg
     8461 accgccgggg ccgacgaagc cgaccgcgtg cgagtcgtcg accatgacca tcgcgccgta
     8521 gcggtcggcc aggtcgcaga tctcccgcag cggcgcgagg tagccgtcca tcgagaacac
     8581 gccgtcggtg acgatgaggg tgcgccgggc accctcggcg tccttcaact gccgctccag
     8641 gtcgtccatg tcgcggttgg cgtaacgcag ccggcgggcc ttggagagcc ggatgccgtc
     8701 gatgatggag gcgtggttga gggcgtcgga gatgaccgcg tcctccgggc cgagcagggt
     8761 ctcgaagaca ccgccgttgg cgtcgaagca ggaggagtag aggatggtgt cctcctggcc
     8821 gaggaagccg gagatccgcc gctccagctc cttgtgcacc tcctgggtgc cgcagatgaa
     8881 gcggaccgag gccaggccgt agccccagcg gtccagggcc ccgtgggcgg cggcgatgac
     8941 ctcggggtgg tcggccaggc ccaggtagtt gttggcgcag aagttaagga cctcgccggg
     9001 gcggccttcc ccaagctctc ggcttccctc gagcagggga gaccccattg cggtgacctt
     9061 gacggtggcg gactgcgggg tgccgatgac ccgctcgggc ttgtgcagtc cggcggcgcg
     9121 gatctcgtcg agggcggtgc gcagatcgtc gcgaacggcg tcgaacatgg ggtggtgtct
     9181 ccttaggcgg ggtcgaggtc gggcgacggg agggcaggcc ggctggcggc gccgtcatcg
     9241 ggggtggcgg ggctcaggcg ctccagtcga ggatgacctt gccgctctgg ccgctggccg
     9301 cgtcggcgaa ggcggtctcg aactcctggt acgggtagcg gccggtgatc acgggggcga
     9361 ggtcgaggcc gccttccagc agcaccgaca tcgcgtacca ggtctcgaac atctcccggc
     9421 cgtagatgcc cttgatcgtg atcatggagg tgacgatccg ggaccagtcg accgggaact
     9481 cctcggcggc caggcccagc acggcgatcc ggccgccgtg cgtcatgttg ccgatcatgt
     9541 cgcgcatggc ctcgggccgg ccggacatct ccaggccgac gtcgaagccc tcgcgcaggc
     9601 ccagttcgcg ctggccggcc tcgatcgagg tgtgggcgac gttgagggcg aggctgacgc
     9661 cgaccttgcg ggccagttcc aggcgctgct cgctgacgtc ggtgatcatc acatggcggg
     9721 cgccggcgtg ccgggcgacc gccgccgcca tgatgccgat cggtccggcg ccggtgatca
     9781 ggacgtcctc gccgaccagc gggaaggaga gcgcggtgtg cacggcgttg ccgaacggat
     9841 cgaagaccgc ggcgatgtcg aggtcgaccg ggacccggtg cacccacacg ttggtggcgg
     9901 gcagcgcgac gtactcggcg aaggcgccgt cccggccgac gcccaggccg acggtggcgc
     9961 ggcacaggtg gcggcggccg gcctggcagt tgcggcagct gccgcagacc aggtggccct
    10021 cgccgctgac cagatcgccg ggcttgatgt cggccacgtc ccggccggtc tcgacgacct
    10081 ccccgacgaa ctcgtggccg acggtcagcg gggtccgaac ggcctgctgc gcccagccgt
    10141 cccaggcccg gatgtgcagg tcggtgccgc agatgccggt gcgcagcacc ttgatcagca
    10201 catcgccggg gccgat
//
