LOCUS       NZ_PNGD01000013        10297 bp    DNA     linear   CON 15-SEP-2024
DEFINITION  Streptococcus sp. UMB0029 ERR1203613.17957_1_25.13, whole genome
            shotgun sequence.
ACCESSION   NZ_PNGD01000013
VERSION     NZ_PNGD01000013.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus sp. UMB0029
  ORGANISM  Streptococcus sp. UMB0029
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 73182)
  AUTHORS   Thomas-White,K., Kumar,N., Forster,S., Putonti,C., Lawley,T. and
            Wolfe,A.J.
  TITLE     Bacterial strain isolated from the female urinary microbiota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 73182)
  AUTHORS   Thomas-White,K., Kumar,N., Forster,S., Putonti,C., Lawley,T. and
            Wolfe,A.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2017) Microbiology & Immunology, Loyola University
            Chicago, 2160 S 1st Ave, Maywood, IL 60153, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Velvet v. 1.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.87x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002871495.1-RS_2024_09_15
            Annotation Date                   :: 09/15/2024 04:52:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,194
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 35 of 102
            Pseudo Genes (incomplete)         :: 71 of 102
            Pseudo Genes (internal stop)      :: 24 of 102
            Pseudo Genes (multiple problems)  :: 25 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:49:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 51743
            Orig. end                         :: 62040
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            PNGD01000013.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10297
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10297
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10297
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:5297](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5297
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            340..3048
                     /locus_tag="CJ239_RS09340"
                     /old_locus_tag="CJ239_09330"
     CDS             340..3048
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /locus_tag="CJ239_RS09340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CJ239_09330"
                     /product="ABC transporter permease"
                     /protein_id="WP_102211356.1"
                     /transl_table=11
                     /translation="MIKRKTYWKDLIQSFTGSKGRFLSILTLMMLGSLALVGLKVTSPN
                     MEATANAYFTTAQTLDLAVMSNYGLDQADQKELEQMQGAEVEFGYLTDVTMDNGQDAIR
                     LYSKPERISTFQIREGRLPQSDQEIALASHLQEQYSVGQEISFKEKEGGHSSLKNHTYT
                     ITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGLNTFS
                     ETYEEKSKQHQEDLEQALSDNGKARLQLLKKEGQESLDKGQETLDKAETNLQEGKRRLV
                     AAQARIQAQESQLALLPQAHREQASAQLTQAKQELSKEEDKLKQAEQNLAQEKEKLEKH
                     QQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASASIRAVGNIFPVVLYAVAAMVTFTTM
                     TRFVDEERTHAGIFKALGYRSKDIIAKFLLYGLVAGTVGTALGSILGHYLLASVISSVI
                     TKGMVVGETQIQFYWTYSLLALGLSWLASVLPAYLVARRELHDEAAQLLLPKPPVKGAK
                     ILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGLGIQSSVAGVP
                     SRQFQQIQQYQMIVSENPSATNQDKVELEEVLKGQDIQAYQKIYSKTLDKDFKGKAGLQ
                     NITLMMTEKEDLTPFIHLQHHQQELTLKDGVVITAKLAQLAGVKVGQTLEIEGKELTVA
                     AIAENYVGHFIYMSQASYEQIYGQLPQANTYLVRLKDSSAPSIESQAGLLMNQAAVSSV
                     VQNASAIRLFDSVASSLNQTMTILVIVSVLLAIVILYNLTNINVAERIRELSTIKVLGF
                     HNKEVTLYIYRETIVLSLVGIVLGLISGFYLHQFLIQMISPATILFYPQVGWEVYVIPV
                     AAVSIILTLLGFFVNHHLRKVDMLEALKSVE"
     gene            complement(3463..4326)
                     /locus_tag="CJ239_RS09350"
                     /old_locus_tag="CJ239_09340"
     CDS             complement(3463..4326)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /locus_tag="CJ239_RS09350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CJ239_09340"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_102211357.1"
                     /transl_table=11
                     /translation="MNTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSAD
                     LLFKLSQRLEVPLDYFFNEQIEIKSNLSNFKKLSARLLGDRNYDNLEYIYKIEIERSTF
                     LTLEDRAYLEWIKAIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFYSLV
                     GREQEYEANYSHLMELYQTKNFEHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEALETI
                     ELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMKIENYL
                     KELDTV"
     gene            4518..4687
                     /locus_tag="CJ239_RS12270"
                     /old_locus_tag="CJ239_09345"
                     /pseudo=""
     CDS             4518..4687
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227862.1"
                     /locus_tag="CJ239_RS12270"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="CJ239_09345"
                     /product="PhrA family quorum-sensing system peptide"
                     /pseudo=""
                     /transl_table=11
                     /translation="MRKKRGIKKLVTFALLGVFTFSNTIPYQQLFRRIDNWKFEYNHKR
                     SPMDLMLGRLI"
     misc_feature    complement(4520..4535)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: medium, score: 24.81"
                     /tool="antismash"
     gene            5001..5297
                     /locus_tag="CJ239_RS09360"
                     /old_locus_tag="CJ239_09350"
     CDS             5001..5297
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911560.1"
                     /locus_tag="CJ239_RS09360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CJ239_09350"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_102211358.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 2.3e-09,
                     bitscore: 29.9, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLKIRKVVASSLAVLAFSLTLPVLAVSHRGGEWTYGGHHDPNNWG
                     AFSNYYHGSRDHWTYVGSNGRDNQKTAYAGARSAAYAFVNTNFGEQVTFDAGW"
     gene            5400..7415
                     /locus_tag="CJ239_RS09365"
                     /old_locus_tag="CJ239_09355"
     CDS             5400..7415
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /locus_tag="CJ239_RS09365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CJ239_09355"
                     /product="DUF1430 domain-containing protein"
                     /protein_id="WP_102211359.1"
                     /transl_table=11
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSHYYPNVVVRDSSPET
                     TFQHVATRLGKLAEETDSFIAIQHQDPNSEGTTVFSYTTFGNGKLPDGLQEKNLEDAQS
                     SSVETNYFVFDGNLDIHLLREELSQLGLTNMHLTIPSKLSTLMAIFSNGFQLISLLIFI
                     LTFVALTLISQISQLRSSGIRLISGEKRWSIFLRPVGEDLKGIAVGFSLAGVLAILMQK
                     ILSLPTQSLMTIGAGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVRGIISL
                     ILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEVWSQERQLTILSISREGTSPGFDEQ
                     AQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNLTTSTEWHDYSPNGNVL
                     IVTPQYLERQNIPVDTTIEQKMNHLDVGEFVLLLPEHLRSEEEHYKSVFEDDLTSRMSS
                     QDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQSVLFWVDAV
                     QNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLSDNIQRERWVMLAGAVLGIAT
                     SILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLGFVASVFLQVEI
                     VVAFLVLLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
     gene            7417..8049
                     /locus_tag="CJ239_RS09370"
                     /old_locus_tag="CJ239_09360"
     CDS             7417..8049
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /locus_tag="CJ239_RS09370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CJ239_09360"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_102211360.1"
                     /transl_table=11
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLMN
                     MIGKLESYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLISQK
                     LSRSEQRLRQKQALEQVGLAYLNLDKRIFELSGGESQRVALAKVILKNPPLILADEPTA
                     SIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVITMDRLK"
     misc_feature    complement(8068..8081)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.41"
                     /tool="antismash"
     gene            8190..>8348
                     /locus_tag="CJ239_RS09375"
                     /old_locus_tag="CJ239_09365"
                     /pseudo=""
     CDS             8190..>8348
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712961.1"
                     /locus_tag="CJ239_RS09375"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="CJ239_09365"
                     /product="competence protein ComA"
                     /pseudo=""
                     /transl_table=11
                     /translation="MKKFDNYIIEKPCDSNSDKLQKISIIESLVDDVLQFSLRINNSVR
                     DIFLLQPF"
     gene            complement(8529..9020)
                     /gene="tpx"
                     /locus_tag="CJ239_RS09380"
                     /old_locus_tag="CJ239_09370"
     CDS             complement(8529..9020)
                     /EC_number="1.11.1.-"
                     /GO_function="GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /gene="tpx"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1196: alkyl hydroperoxide reductase/ Thiol specific"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /locus_tag="CJ239_RS09380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CJ239_09370"
                     /product="thiol peroxidase"
                     /protein_id="WP_061087714.1"
                     /transl_table=11
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKKK
                     VLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGIENAIMLS
                     DYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAVAAAKAL
                     "
     misc_feature    complement(9625..9638)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 21.73"
                     /tool="antismash"
     misc_feature    complement(9919..9932)
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: weak, score: 15.78"
                     /tool="antismash"
ORIGIN
        1 tcttgataga tgtaggtctg actcatcgta tcaataattt cccagcccag ctttctggag
       61 gggagcaaca gcgagtctcc attgcacgcg cggtagctaa aaatcctaaa cttctccttt
      121 gtgatgaacc aactggagcc ttggattatc agacgggcaa gcaagtcttg aaaattctcc
      181 aagacatgtc tcgtcaaaag ggagcgacgg tgatcattgt gactcataat agcgcgctag
      241 ctcctattgc ggatcgcgtg attcatatgc atgatgccag tgtcaaggat gtggtgatta
      301 accagcatcc tcaggatatt gacagtttgg agtactagca tgatcaaacg aaaaacctat
      361 tggaaggact taattcagtc cttcacaggc tccaaggggc gttttttatc catcttgacc
      421 ttgatgatgt tgggctctct agccctagta ggtctcaaag tgactagccc caatatggag
      481 gcgactgcca atgcttattt cacaactgct caaaccttgg atttggcagt catgtctaac
      541 tatggcttgg atcaagcaga tcaaaaagaa ctagaacaga tgcagggtgc agaggttgag
      601 tttggctatt tgacagatgt gactatggat aatgggcagg atgccattcg gctgtactcc
      661 aaaccagagc gaatttcaac ctttcagata agagagggac gacttccgca gtcagaccag
      721 gaaatcgctt tggcaagtca tctgcaggag caatacagtg tgggacagga gattagtttt
      781 aaagaaaaag aaggcggtca ttcctcttta aaaaaccata cctataccat tactggtttt
      841 gtggattcgg ctgaaatcct ttcccagcga gatatgggct acgcaggaag tggaagtggg
      901 actctgacag cctatggggt gattttacct agccagtttg atcagaaagt ctacaatata
      961 gctcgtttga aatatcaaga tttagcaggc ttaaatactt tttcagaaac ttatgaagag
     1021 aaatccaagc aacatcagga agaccttgaa caagctttat cagataatgg caaggcacgt
     1081 ttgcaactct tgaaaaaaga aggacaagag tctctagaca aaggtcaaga gacccttgac
     1141 aaggctgaga ctaatttgca ggaaggcaag cgtcgtttag tagctgctca agctcgtata
     1201 caggctcaag aaagtcaact agccttgctt cctcaagccc atagagagca ggctagtgct
     1261 caacttaccc aagccaagca ggaattgagc aaggaagagg acaaactaaa gcaggctgaa
     1321 caaaatctag cccaagaaaa ggaaaaatta gaaaaacatc agcaagtctt ggatgatttg
     1381 gctgagccaa ggtatcaggt ctataatcgt cagaccatgc ctggcggtca aggctatctc
     1441 atgtacagca atgcttcagc cagtattcga gcagtgggca atatctttcc ggtagtgctt
     1501 tatgccgtag cagccatggt gacctttacc accatgactc gctttgtgga tgaagaacga
     1561 acacatgcag ggatttttaa ggccttgggt tatcgtagca aggatattat tgccaagttt
     1621 ctcctttacg gtctagtagc tgggactgtc ggaacagctc taggtagtat acttggccat
     1681 tatttactag ccagtgtgat ttcaagtgtc attacaaaag gcatggtggt gggagaaacc
     1741 cagattcagt tttattggac ctatagtttg ctggctcttg gtttaagttg gttggcgagt
     1801 gtcttaccag cctatctggt ggctcggagg gaacttcacg atgaagcagc ccagctttta
     1861 ctgcctaaac cacctgtcaa aggagctaaa atcttactgg agcgcatcgg ttttatctgg
     1921 cgtcgtctca gttttaccca taaggtaaca gcccgcaata tcttccgtta taagcagaga
     1981 atgttgatga cgatctttgg tgtggctggt tcagtcgctc tgctctttgc aggcttggga
     2041 atccaatcct ctgtagcagg agttccgtct agacagtttc aacaaatcca acagtatcag
     2101 atgattgtct ctgaaaatcc tagtgcgacc aatcaagaca aggtagagct agaagaagtg
     2161 ttgaaagggc aggatatcca agcctaccag aaaatctatt ctaaaacgct agacaaggat
     2221 ttcaaaggca aggctggtct tcagaatatc acccttatga tgacagagaa agaagattta
     2281 actcctttta tccatctgca acatcatcag caggagctga ctttaaaaga tggtgttgtc
     2341 atcacagcta aactagccca gttggcaggt gttaaggttg gacagacttt agaaattgaa
     2401 ggtaaggagc taacggtcgc tgccattgct gagaactacg ttggtcactt tatttatatg
     2461 agtcaagcta gctatgaaca aatttacgga cagctacccc aagccaacac ttatctggtt
     2521 aggctaaagg attctagtgc gcctagtatc gaaagtcagg caggcttact tatgaatcaa
     2581 gctgcggtgt ccagtgttgt tcaaaatgct tcagccattc gactctttga ctctgtcgct
     2641 agctcactca atcagaccat gaccatcctg gtcatcgtgt cggttctatt ggctattgtt
     2701 atcctctaca atctgaccaa tatcaatgtg gctgagagaa tccgtgaact ttctactatc
     2761 aaggtgctcg gctttcataa taaagaagtc actctttaca tttatcgtga gacgattgtc
     2821 ttgtcccttg tgggaatcgt acttggtttg atctcaggtt tctatttaca ccaatttttg
     2881 attcaaatga tttcgcctgc gactattctc ttttatccgc aggtaggttg ggaagtctat
     2941 gtaatcccag tggcagcagt aagcattatt ttgaccctgc tcggtttctt tgtcaatcat
     3001 catctgagaa aggttgatat gttagaagcc ttgaaatctg tagagtaata ctattcgaaa
     3061 atctcttcaa accagatcag ctatatctac accctcaaaa cagtgtcttg agtagcctgt
     3121 ggctagtttc ctagtttgct ctttgatttt cattgagtat aaggtagttg tttttatctg
     3181 attgaactta tatttactcg taaatcatga ccacccgtca aacgggtggt ttgtcccagg
     3241 actataagcc caaattacca gccagcgcct aaagacgctg gctttcacgt tgttcaagcc
     3301 tcattgctct tgactcgtca cttgcctctc aaagaggctt tagtattact taccactatc
     3361 cctaaaggaa tcttcacata attataaggc tgatagtatt aatttcacta tcagccttac
     3421 aggttattta acgtttcaga aaaactataa tgtcaagatt aactaaacag tatctaattc
     3481 cttcaaataa ttttctatct tcatcaacat taaaggattg ttataaatct tacataactc
     3541 tcttgcttct atataataat ttttgacttg ttctctgtct agaaatttgg ctccagcatt
     3601 tcctacaaga ataagtagag gagccagttg gtagcttgtc tgtctttgtt tacagagttc
     3661 aatcgtctca agagcttctt ggatggcttc gttatatttt tccttcgaca ctaggtagtg
     3721 agcgtagttg taacgaactc tgatgtaccc aaataaaaac tcttgatgct caaaattttt
     3781 tgtctgatat aactccatca aatgagagta gtttgcctca tattcttgtt cacgacccac
     3841 taaggaatag aaattagata gagtattcaa ggcctttaaa taaatcagag tatttgaaga
     3901 gacttttaat aatatatttt ccaatgaaga aatagcctca cacttactgt catattgata
     3961 gaagtcaata atagctttaa tccattcaag gtaagctcgg tcttctagtg ttagaaaggt
     4021 gcttcgctca atctctattt tataaatata ttccaaattg tcataatttc tgtcacctaa
     4081 tagtcgagca gataatttct tgaaattaga gaggttagat ttaatttcaa tttgttcatt
     4141 aaaaaaataa tctaatggta cttcaagtcg ttgtgatagt ttgaacaaaa ggtctgcgga
     4201 gggaatgaaa tgccctctct caattttact aatctggctt tgttcacaaa ttccttctgc
     4261 aagagtttgt tgggagagtc tcaactcttt tcttttcaat ctgaatttct ctgcgagtgt
     4321 attcattaaa gataataatc cttcctattt gcttaatttc attataaaat ttttagttca
     4381 aaatagcaag ttaaagaaat aaataattct ttatggtaat aaaaaaacaa aaaagtcaaa
     4441 aattacataa aaatgcttgg ctaaataaaa tatatctgtt aaaataaaaa caaggaaaca
     4501 gaaaagggtt tcattgcatg agaaaaaaac gtggaattaa aaaattagtt acatttgcat
     4561 tgctaggtgt ttttacgttt agtaacacaa ttccttacca acagttattc agaagaatag
     4621 acaattggaa gttcgagtac aatcacaaaa gaagtccaat ggacttgatg ttgggaaggc
     4681 tgattgattt cgtattactt taaaattcaa ctatttagag aaaggtattc ttatgagagc
     4741 ttgtaacaat accagataga tataagaatg gagatgtttg ataaaatgct ttcatagcta
     4801 accaactgtt gaaaattatg agttacaaaa tctaaagtat ttaagattag tttataaaat
     4861 taggtctctg cctgtagaaa ggaacgtgca gttagaaagg aggaaaaaat aggatactat
     4921 aattcagact gagatttcta attaaaatat gtagttgcat tttgctcatt ttataatatt
     4981 tcatttagag gagtaataat atgttaaaaa ttagaaaagt agttgcatcc tcccttgccg
     5041 ttttggcatt tagtttaact cttcctgttt tggcagtttc tcaccgtggt ggcgagtgga
     5101 cctatggagg ccatcatgat ccaaataatt ggggagcatt ttcgaattat tatcatggtt
     5161 cacgggatca ctggacctat gttggaagta atggacgtga caatcaaaaa acagcttatg
     5221 caggagcacg tagtgcggcc tatgcgtttg ttaatacaaa ttttggggag caagttacat
     5281 ttgatgcggg atggtaaaga ataattgaag aagattcttt tatcaatagt ttagtaagct
     5341 tgttagttta tagagggcag gggtatttct gccctctgct ttagaataag gaatgcacta
     5401 tgaaaaaatt atttatactc atatccaacc tgcttgctag tttgttcttt gtttgggtat
     5461 ttaccatttg gactgataca tatgtctccc attattaccc caacgttgtt gtacgtgatt
     5521 cttcccctga aacaactttt cagcatgttg ctacacgctt gggaaaacta gcagaagaaa
     5581 cggatagctt tattgcgatt caacaccaag atcctaactc agaaggtact acagtttttt
     5641 cttatacgac ctttgggaac ggaaagttgc ctgatgggct tcaggaaaaa aatctagaag
     5701 atgctcaaag tagtagtgtt gaaacaaact attttgtatt cgatggaaac ctagatattc
     5761 acttgctaag ggaggagcta agtcaacttg gcttgactaa catgcacctg acaattccat
     5821 ctaaactatc tacgttaatg gctatcttta gtaacggatt tcagttaatc agtttattga
     5881 ttttcatctt aacctttgta gcactgactt taattagtca aattagccaa ttacggtcgt
     5941 cgggcattcg cctcatttca ggagagaaac gatggtccat tttccttaga ccagttggtg
     6001 aggatttgaa agggatagct gttggtttca gtttagctgg cgtgctcgcc attcttatgc
     6061 aaaaaattct gtcgcttcca acgcaatccc taatgaccat aggagcaggc ttgctcagtt
     6121 ataatctcat cttgttgtcg atctccctgt ttttcgctca actcttcgca gttgggataa
     6181 aaaagataca cctgatgcag attataaaag ggcaagtgcc tgtcagagga atcattagtt
     6241 taattttaat cggtcaacta ttagcgatta ttattgtaac gctgggaata gggagtagtt
     6301 taaagtattc ccaagcctgg caacagcatc ggattggaca agaggtttgg agtcaggaaa
     6361 ggcaactgac tatcctatca atcagccgtg aaggaacgag tcctggtttt gatgaacaag
     6421 ctcaaagaaa actcagaact tggtatcaat tgatggatct ggctgtttca gaacaaaagg
     6481 ctttcttatc tagacaccag ttaattgacc gtactttgca aaatggcatg gcttcctcca
     6541 aaaacttgac aacctctaca gaatggcacg attacagccc gaatggcaat gtccttatcg
     6601 tcacaccgca atacttggag cgtcaaaaca ttcctgtaga tacaactatt gagcaaaaga
     6661 tgaatcacct tgatgtaggg gagtttgtct tattactgcc tgaacacctc cgttcagagg
     6721 aagaacatta taaatctgtt tttgaagacg acttaaccag tcgcatgtct agtcaagatg
     6781 aacgacagca aatgactgct acggtaggtt atttggaatc aggtcaggat cgttttgtgt
     6841 ataatacgac ccctatttct taccagcagt ttttgaaaga tccaatcatc attgttataa
     6901 caccccaatc aactggtcca cagtccgttt tgttttgggt agacgcagta cagaactacg
     6961 ttctctttaa tcaattgtct gatgcccagg agcttatcca gagacaaggc attgaaaatt
     7021 gggtctcaga aatgcaaaca ggttaccaca actacatcac attatcggat aatatccaga
     7081 gggaacgttg ggtaatgcta gcaggagctg tgcttgggat tgcaacttca atcttgttgt
     7141 ttaacactat gaataggctc tactttgaag aatttagacg tgctattttt atcaaacgca
     7201 ttgcaggtct caggttctta gaaatccatc gcacttatct ctttgctcaa ctgggtgtgt
     7261 ttttactggg atttgttgcg agtgtatttc ttcaggtaga gatagtagtt gctttcttag
     7321 tcttgttact cttcactggt ctatctcttt tacagttaca tgtccaaatg cagaaagaaa
     7381 acaagatgtc catgcttgtt ttgaagggag gttaatatga ttgaacttaa acaggtgagt
     7441 aaatcttttg gagaacgaga gttattttcg aatctttcaa tgacatttga ggctggaaaa
     7501 gtctatgcct taattggttc aagtggtagc ggtaaaacaa ccttgatgaa catgattggg
     7561 aaattagaat cttatgatgg gacgattttt taccgaggta aagacttggc aaattataaa
     7621 tcgagtgatt ttttccgtca cgaattgggc tacctcttcc agaactttgg cttaattgaa
     7681 aaccaaagta ttgaagaaaa ccttaagcta ggtctcatta gtcaaaagtt gagtcggtcg
     7741 gaacagcggt tgaggcagaa gcaggcttta gaacaggtcg gcctggctta tcttaaccta
     7801 gataagcgca tctttgagtt atcgggcgga gaatcgcaac gggttgcctt ggcaaaagtt
     7861 atcttaaaga atccacccct tattctggca gatgagccaa cagcttcaat agacccagca
     7921 acctctcagt tgattatgga gattttgcta tctcttcgag atgataatag gctaattatt
     7981 atcgcaacac ataatccggc aatttgggag atggctgatg aagtgatcac gatggatcgt
     8041 ctgaaataaa aatccttgtt tttaattgca cgatgagtta ctgaaatatt atcatgaatc
     8101 aataattgga gttaatttag aattgtactt tattaatatt gaggtaactt tttcttgata
     8161 aaggaagaaa tagtggagag gaagttagat tgaaaaaatt cgacaattat attattgaga
     8221 agccttgcga ttctaattca gataaactgc aaaaaatctc aataattgaa agtttggtag
     8281 atgatgtttt gcaattttct ctcagaatca ataatagtgt aagagatatt ttcctcctac
     8341 aaccgtttca aaagaaatat ttacccattg ggagaagatg ttgcagtgga taatgtgtta
     8401 ctcactgaaa aatataattt tcaactaaaa gattttgaag gattcatttc aaaataaaca
     8461 ataattaaag aataagaaaa atcctccgtt tcaaagagta gggaactctt tgtgacagag
     8521 gattttttct atagggcttt agcagctgca actgcggctt cgaagtttgg ttcagagttg
     8581 atattgtcca cgtattcaac gtagcgaatc gtattgtcag tatcgaggac aaagactgcg
     8641 cgtgccaata ggtgccactc attgattaaa agtgcatagt cacgcccaaa ggaatggtcg
     8701 aaatagtctg agagcatgat ggcattttca ataccttcag caccacacca acgtttttga
     8761 gcaaaaggta ggtccatgga aacagtcaat acgaccgtgt tgtccagtcc agcaagttct
     8821 tcattaaagc gacgcgtttg agttgagcag atacctgtat cgatagaagg aacgacactc
     8881 aagacttttt tcttgccatc aaaatcagcc agagattttt tagaaaggtc tgttgtagta
     8941 agagaaaaat cgagcgcctt gtcgccgact tgtagttgtt tacctgtaaa gctcacagga
     9001 tttccgagaa aagttaccat aggatactcc aatctttttt cttccatttt agctgaaacg
     9061 gtcagaattt tccaatgatt tgactggaaa tatgggcata gaaaaaacgc cagctcatgt
     9121 gagaatgacg tttttcagat tttttatcta tttgatattt ctcgaaggta gtgcggacgg
     9181 gagcaacttc ctttggaatt tcatccctaa tttttgagcc ttaggtctca aaaattccgt
     9241 cctagataag atcaaagttc ttatctagga taccactaca gtgagaaata tctggtaaaa
     9301 actcacttcg ttcgtactga tttaagaaat atttattctt ataaacgctt tctttagcta
     9361 gagatagaac taacaaagct aatattagaa gtaatgtaaa gagagacaag aacctagtaa
     9421 tagataaact aatatctaga ttattttgcc aatccttcag caatcttgtc aaggttgtat
     9481 ttcatcatgt tgtagtagct gtcgccttct ttaccttgtt ctgcgataga gtcagtaaag
     9541 atttgtgcgt agattgggat gtttgtgtct tgtgaaacag tcttcattgg acggtcatcc
     9601 acacttgatt ctacaaagag tgatggaact tttgtttggc gaagtttttc aaccaaggtc
     9661 ttgatttgtt caggagttcc ttcttcttca gtattgattt cccagatgta ggcacttggg
     9721 acaccatagg ctttagagaa gtatttgaat gctccttcgc tggttacgat gagtttcttt
     9781 tcagcaggga tgttattaaa tttatcctta ctttctttat caagtttgtc taacttatca
     9841 gtatattctt tgagattttt ttcgtagaat tccttgttgc taggatcttt agcgctcaat
     9901 tgtttggcaa tatttttagc aaagataatt ccattttcaa ggttaagcca agcgtgtggg
     9961 tcttctttac ctttttcatt ttttccttca aggtagataa catcaacgcc ttcgctgact
    10021 gcaaagtagt ctttgttttc agttttctta gcattttcta ccaatttgct aaaccaagca
    10081 ttgccacctg tttcaaggtt gataccgtta tagaaaatga ggtcagcctc agaagttttc
    10141 ttaacatctt cagggagtgg ttcgtattcg tgtgggtctt gtccaatcgg aacgatactg
    10201 tgaagatcaa tcttgtcacc agcaatattt ttagtaatat cagcgatgat tgagtttgta
    10261 gcaacaactt ttagtttttg accagaagct gcatctt
//
