LOCUS       NZ_PNGD01000009        12154 bp    DNA     linear   CON 15-SEP-2024
DEFINITION  Streptococcus sp. UMB0029 ERR1203613.17957_1_25.9, whole genome
            shotgun sequence.
ACCESSION   NZ_PNGD01000009
VERSION     NZ_PNGD01000009.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus sp. UMB0029
  ORGANISM  Streptococcus sp. UMB0029
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 125000)
  AUTHORS   Thomas-White,K., Kumar,N., Forster,S., Putonti,C., Lawley,T. and
            Wolfe,A.J.
  TITLE     Bacterial strain isolated from the female urinary microbiota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 125000)
  AUTHORS   Thomas-White,K., Kumar,N., Forster,S., Putonti,C., Lawley,T. and
            Wolfe,A.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2017) Microbiology & Immunology, Loyola University
            Chicago, 2160 S 1st Ave, Maywood, IL 60153, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Velvet v. 1.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.87x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002871495.1-RS_2024_09_15
            Annotation Date                   :: 09/15/2024 04:52:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,194
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 35 of 102
            Pseudo Genes (incomplete)         :: 71 of 102
            Pseudo Genes (internal stop)      :: 24 of 102
            Pseudo Genes (multiple problems)  :: 25 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:49:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 67001
            Orig. end                         :: 79155
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            PNGD01000009.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..12154
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..12154
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12154
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:7154](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..7154
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    265..278
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.28"
                     /tool="antismash"
     misc_feature    266..279
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.97"
                     /tool="antismash"
     misc_feature    391..404
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.28"
                     /tool="antismash"
     misc_feature    517..530
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.28"
                     /tool="antismash"
     misc_feature    518..531
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.97"
                     /tool="antismash"
     misc_feature    643..656
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.13"
                     /tool="antismash"
     misc_feature    678..693
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 22.03"
                     /tool="antismash"
     gene            827..1006
                     /locus_tag="CJ239_RS07415"
                     /old_locus_tag="CJ239_07405"
     CDS             827..1006
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180832.1"
                     /locus_tag="CJ239_RS07415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CJ239_07405"
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="WP_000180832.1"
                     /transl_table=11
                     /translation="MTNFDKMEQNFVALTEEELVNVDGGFFGPVLFTIWGTKVLVGHAL
                     SAGVAVGFAALATN"
     gene            1026..1220
                     /locus_tag="CJ239_RS07420"
                     /old_locus_tag="CJ239_07410"
     CDS             1026..1220
                     /GO_function="GO:0005186 - pheromone activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015035.5"
                     /locus_tag="CJ239_RS07420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CJ239_07410"
                     /product="ComC/BlpC family leader-containing
                     pheromone/bacteriocin"
                     /protein_id="WP_084890458.1"
                     /transl_table=11
                     /translation="MNYNFENMKSDELEKISGGSVVGALALAWGVTKAVGIIGGTAVSV
                     YVAGKSVKAIGDWGLSRLR"
     gene            1247..1468
                     /locus_tag="CJ239_RS07425"
                     /old_locus_tag="CJ239_07415"
     CDS             1247..1468
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787045.1"
                     /locus_tag="CJ239_RS07425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CJ239_07415"
                     /product="hypothetical protein"
                     /protein_id="WP_084890459.1"
                     /transl_table=11
                     /translation="MKNEITKVIKIVLLFVSMAIMNLMLLGILAMLGFSLTGSVASYLV
                     PPLFSTLVLLFINKMITRLDRGKNKSLL"
     misc_feature    1810..1823
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.31"
                     /tool="antismash"
     misc_feature    complement(1976..1990)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: medium, score: 23.81"
                     /tool="antismash"
     gene            2102..2290
                     /locus_tag="CJ239_RS07430"
                     /old_locus_tag="CJ239_07420"
     CDS             2102..2290
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180826.1"
                     /locus_tag="CJ239_RS07430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CJ239_07420"
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="WP_000180826.1"
                     /transl_table=11
                     /translation="MTNFDKMEQNFVALTEEELMNVDGGIAPLVVAFGVAAFKGFSVTG
                     GAIILGAGLYQVGKNLK"
     gene            2499..2678
                     /locus_tag="CJ239_RS07440"
                     /old_locus_tag="CJ239_07430"
     CDS             2499..2678
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF021923.5"
                     /locus_tag="CJ239_RS07440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CJ239_07430"
                     /product="Blp family class II bacteriocin"
                     /protein_id="WP_102211093.1"
                     /transl_table=11
                     /translation="MNILVNDNQFTAMTEKELATVNGGVLPAAVVAVPVGIKVAGYVVG
                     SLSAAGLVAWGYFR"
     gene            3164..3331
                     /locus_tag="CJ239_RS07450"
                     /old_locus_tag="CJ239_07440"
     CDS             3164..3331
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284951.1"
                     /locus_tag="CJ239_RS07450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CJ239_07440"
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="WP_001047583.1"
                     /transl_table=11
                     /translation="MNLEQFYQLSDDELVGTKGGFGLAEAVGIAGVLNAAVQIFNAGYK
                     FGSDLARRGR"
     gene            3335..3550
                     /locus_tag="CJ239_RS12205"
                     /old_locus_tag="CJ239_07445"
     CDS             3335..3550
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009755479.1"
                     /locus_tag="CJ239_RS12205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CJ239_07445"
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_102211095.1"
                     /transl_table=11
                     /translation="MNNRNINLNTLLPKLATICPLLGITLNVALHVIRTGSLVSFNWQW
                     TLVGLLFAGYFGIFRKDLSKNNQNYK"
     gene            3631..3810
                     /locus_tag="CJ239_RS12210"
                     /old_locus_tag="CJ239_07450"
     CDS             3631..3810
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846993.1"
                     /locus_tag="CJ239_RS12210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CJ239_07450"
                     /product="hypothetical protein"
                     /protein_id="WP_042900083.1"
                     /transl_table=11
                     /translation="MKTFLLGFAEGYFTVSLIIYIATYLMLKNPINTLFYPEIYPVLFG
                     LFYLGYKVNKKQVE"
     gene            3889..4095
                     /locus_tag="CJ239_RS07465"
                     /old_locus_tag="CJ239_07455"
     CDS             3889..4095
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869802.1"
                     /locus_tag="CJ239_RS07465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CJ239_07455"
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_102211096.1"
                     /transl_table=11
                     /translation="MKFVKSILKVLPEIMVLLSSMSYIIIRLVADINKVSLPTWFDNLN
                     IPTVALFMMVFILLYRSKEKKNR"
     misc_feature    complement(4367..4380)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: strong, score: 23.31"
                     /tool="antismash"
     gene            4503..4691
                     /locus_tag="CJ239_RS12215"
                     /old_locus_tag="CJ239_07460"
     CDS             4503..4691
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269957.1"
                     /locus_tag="CJ239_RS12215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CJ239_07460"
                     /product="hypothetical protein"
                     /protein_id="WP_000757184.1"
                     /transl_table=11
                     /translation="MKKVLRYFLVFVFLFLMNIFIFKILATLGFQLTMSEKSYIVPPLF
                     SIIVLYMIDKRIRKKKK"
     misc_feature    complement(4707..4720)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: strong, score: 22.76"
                     /tool="antismash"
     gene            5001..7154
                     /gene="comA"
                     /locus_tag="CJ239_RS07475"
                     /old_locus_tag="CJ239_07465"
     CDS             5001..7154
                     /codon_start=1
                     /gene="comA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /locus_tag="CJ239_RS07475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CJ239_07465"
                     /product="peptide cleavage/export ABC transporter ComA"
                     /protein_id="WP_102211097.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 2.7e-43, bitscore:
                     140.2, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 0.0, bitscore: 1126.5,
                     seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYSLAHLRELAKTT
                     MDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGRLLHYYVVTGQD
                     KDSIHIADPDPGVKLTKLPRERFAEEWTGVTLFMAPSPDYKPHKDQKNGLLSFIPILVK
                     QRGLIANIVLATLLVTLINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYILQQIL
                     SYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSIIDALAS
                     TILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNRDTMEAN
                     AVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQKALKKVAH
                     LLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQTAQVANNRL
                     NEVYLVASEFEEKKTVEDLSLMKGDMIFKQVYYKYGYGRDVLSDINLTIPQGSKVAFVG
                     ISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQPYVFNGTILE
                     NLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISGGQRQRIALARA
                     LLTDAPVLILDEATSSLDILTEKRIVDNLMSLDKTLIFIAHRLTIAERTEKVVVLDQGK
                     IVEEGKHADLLAQGGFYAHLVNS"
     gene            7167..8516
                     /gene="comB"
                     /locus_tag="CJ239_RS07480"
                     /old_locus_tag="CJ239_07470"
     CDS             7167..8516
                     /codon_start=1
                     /gene="comB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /locus_tag="CJ239_RS07480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CJ239_07470"
                     /product="competence pheromone export protein ComB"
                     /protein_id="WP_102211098.1"
                     /transl_table=11
                     /translation="MKAEFLESAEFYNRRYHNFSSRVIVPMSLLLVFLLGFATFAEKEM
                     SLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGELLVQYQEGAEGVQAEAYSS
                     QLDMLKNQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYLSQAASLRASTSQQNETI
                     ASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGASLASQNLVYSLYQSYKSQSE
                     ENSQAKSQAVAQVEAQLSQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQHLAKV
                     GQELTLLDQKILETESGKKVQGNLLDKGKITASEDGVLHLNTETSDSIMVAEGALLAQL
                     YPSLEKEGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAKNQLFLDSTITSIDATATKTE
                     KGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            8792..9499
                     /gene="purC"
                     /locus_tag="CJ239_RS07485"
                     /old_locus_tag="CJ239_07475"
     CDS             8792..9499
                     /EC_number="6.3.2.6"
                     /GO_function="GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="purC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312208.1"
                     /locus_tag="CJ239_RS07485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CJ239_07475"
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="WP_102211099.1"
                     /transl_table=11
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAGK
                     GVLNNQISSFIFEKLNAVGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSKRF
                     GVDEGIALDTPIVEFYYKNDDLDDPFINDEHVKFLQIADDQQIAYLKEETRRINELLKV
                     WFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLGELTD
                     VYEIVWEKLQGLK"
     gene            9501..9644
                     /locus_tag="CJ239_RS12385"
                     /old_locus_tag="CJ239_07480"
     CDS             9501..9644
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605805.1"
                     /locus_tag="CJ239_RS12385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CJ239_07480"
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="WP_080710150.1"
                     /transl_table=11
                     /translation="MLATENLRLSTKRTQAEKVPQTFSLRRGLGELTDVYEIVWEKLQE
                     LK"
     misc_feature    11033..11048
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 20.83"
                     /tool="antismash"
     misc_feature    complement(12090..12105)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 20.85"
                     /tool="antismash"
ORIGIN
        1 aggccattga agagatgact gcgacagaag tgaccctgtt agaaggcttg cacttccccg
       61 tatcacggac agctagaaga acccttaaaa aacatctcaa tgtctaatat ctgttagatt
      121 tgaatgaatt tatcaataag aatgtcggaa aattttctga tattcttatt tttttgactt
      181 ctttttcgaa tagataagta agaggaaaag gaaaggagcc tagttcatgt ttatatcaat
      241 gttctaccca tggtttactg agttattttt taaataaaat ttaatttagt tgttcatttt
      301 tgaaattttt ttcgaataga taggtaagag gaaacagaaa ggagcctggt ttatgtatct
      361 accaattttt tatccgtggt ttactaaatt attttttaaa taaatttaat ttagttgtac
      421 attttcgaaa tttttttcga atagataagt gagaagaaaa aggaaagaag tttggtttat
      481 gtatctacca attttctacc cgtggtttat taaattattt tttaaataaa atttaattta
      541 gttgtacatt ttcgaaattt ttttcgaata gataagtaag aggaaacaga aaggagtttg
      601 cttcatgtat ctaccaattt tctatccatg gtttactaaa ttatttttca aataaaattt
      661 aatttagttg tacattttcg aattcttttt cgaataaata agtaagaaag aaaaggaagg
      721 agattagaaa tgcttatttc agttatttat ccaatctggc tatttaagtg gcttagaggc
      781 taaacattaa agatataaca aaacataaaa attgaggaga aaaaagatga caaattttga
      841 caaaatggaa cagaactttg tagctcttac agaagaagag ttggtgaatg tggatggggg
      901 attctttggt cctgtattgt ttactatttg ggggacaaaa gtattagtcg gccatgctct
      961 atcagcaggt gtagcagtag gttttgctgc tctagctact aattaataga tgaaggggat
     1021 agataatgaa ttataacttt gaaaatatga aatcagatga attggaaaaa atttcaggtg
     1081 gctctgtagt aggtgctctt gcgttagcat ggggtgtaac caaggcggtt ggaatcattg
     1141 gtgggacagc tgtttctgtt tacgttgcag gaaaaagcgt taaagcaata ggcgattggg
     1201 gactttcaag attgcgataa ttttttaata cttatgaggt ggtgcaatga aaaatgaaat
     1261 aactaaagta ataaaaattg ttttattgtt tgtctctatg gctatcatga atttgatgtt
     1321 gcttggaata ttagctatgc ttggtttttc tttgacgggc agtgtagcta gttacctcgt
     1381 gccaccttta ttttctactc ttgtgttgct cttcattaac aagatgataa caaggttaga
     1441 cagagggaaa aataagagct tgctatgaaa aaatttttat taggttttgt tttaacagct
     1501 ttcagcatat tattaattta ttctctattt aatttagaaa aggtatcgtt ggagagctgt
     1561 attttgttag tagcattgac atactatatt aataaaaaag aaacatttaa acccaaagtg
     1621 aattagacta agaatatttt aggaaacgat catcagttta cagcaatgac agaaaaagaa
     1681 cgctaataaa tgaaaacaag aacagagtaa aaagtctcgt aaaaggtata ttatcggaga
     1741 tttatctgct gtagatctag ttgcatgggg ttactctatc taagataaaa cactttggat
     1801 aaatcataaa ttttcaaata aaatttaatt tagttgtaca ttttcgaatt atttttcgaa
     1861 taaataagtg agacgaaaag gaaggagatt acgcgatgta tttaccaatc ttattgccat
     1921 ggtttatcaa atggtattta aaataaatga aatttacctg ttcattttaa atcacttctc
     1981 aaatagataa gtgagacgaa aaggaaggaa aaggaattca gtaatttatc tattctggtt
     2041 tttaaaatgg tttaagagtc agaaatcgta aactaaaaaa tagaaataaa ggagaaaaaa
     2101 gatgacaaat tttgacaaaa tggaacagaa ctttgtagct cttacagaag aagagttgat
     2161 gaatgtagat gggggaattg caccactagt agttgcgttt ggagtagctg cgtttaaagg
     2221 tttttctgta acaggagggg ctattatact gggagcaggt ctttatcaag ttggaaaaaa
     2281 tctgaaataa gatcttgagt aagttttatg aatagaatat taaaaaaaat atatatagga
     2341 tctgctttaa tagcttacag catattatta acttattctc tacttaattt agagaaggta
     2401 tcgttgggga tctgtatttt gttagtagca ttgacatact atattaataa aaaagaaata
     2461 tttaaagcaa aagtgaataa gagcaaggag aacttaaaat gaatatttta gtaaatgata
     2521 atcagtttac agcaatgaca gaaaaagaac tagcaacagt taatggagga gtacttcctg
     2581 cagcggtagt agcagttcca gtaggaatca aggttgcggg gtatgttgtc ggcagcttat
     2641 ctgctgcagg gctagttgca tggggttact ttagataaga cactttggag aaatcaatga
     2701 atttttcaaa cgaatttgcg gctgttatta tatcagcttc agcaatagtg ggtttattag
     2761 taggcgtagc tattggatta gctagtatta tttaaagagg ggtataacat gcaaaatttg
     2821 aatcaatttg aaatcgtaga gcaaagtgaa taagttggag caggtttatt aattgttatc
     2881 aaaggattat tacagtagct atagggtatt atgcgaatag accataatct gattgtagtc
     2941 agagaggtat ttctatgttt ttaaaaaaag aagtgatatt actaggggta ttgttatcga
     3001 tacgtattat ttttactatc gttcaaattt taattgatta taaaatatta tctggacagt
     3061 atagtataaa ttttatggat atttcagaag tatttactta tccaatactc attttaatca
     3121 tttacatagc aataaaatat aatacaaagg agtaatgacg aacatgaact tagaacaatt
     3181 ttatcaatta tctgacgatg aattagtagg aacaaaaggt ggttttggtc ttgcggaagc
     3241 agttgggatt gcaggagtac tgaatgctgc tgtacaaatt tttaatgctg gttacaaatt
     3301 tggttcagat ttggctcgtc gtgggcgtta gataatgaat aatcgcaaca ttaatctcaa
     3361 tacattgtta cctaaactag caactatatg tcctttgttg ggaataactc ttaatgtcgc
     3421 gcttcatgtg attcgtacag gttcgttagt atcctttaat tggcaatgga ctttagttgg
     3481 tttgcttttc gcaggatatt ttggtatttt tcgcaaagat ttgtcaaaaa ataatcaaaa
     3541 ctataagtga agaacatgaa tcaaaaggat tttgtgcatg tgaatggtag cgtttttccg
     3601 ccattcttgt caggtaatta ggagtttctt atgaaaacat ttttattagg attcgctgag
     3661 ggatatttta ccgtatcgct cataatctac atcgcaacgt atttgatgtt aaaaaatccg
     3721 atcaatacct tattttatcc agaaatttac cccgttttat ttgggctctt ttatctaggg
     3781 tataaggtca ataaaaagca agtagagtga gcagttaaga atgacttgtt agtctgcgct
     3841 gaaaatagct aggctagaat ctaagaatgc atcacattgg agtttaatat gaaatttgtt
     3901 aaatcaatac tgaaagtttt gcctgaaatt atggtattac ttagttcaat gtcttatatt
     3961 atcatcagat tagtagctga tataaataaa gtatctttac ctacttggtt tgataatctt
     4021 aatataccca cggttgcatt atttatgatg gtattcattc ttttatatag aagtaaagaa
     4081 aaaaagaata gatagatttg aagcggtaga caatctatgg ggaaatagaa atcttatagt
     4141 aacgaatcaa aaaaaggagt aactatgaat cgtaatttag aacggtgtta tctattctga
     4201 ctaggaatgg atcataccag aggtggctta gaaatagcag ggacattaga aattgaagta
     4261 ataaatagga tgtcacaaat gttactatca atgatttatt tgttccaagt ttgcctaggg
     4321 tgtcagtaaa aaatcaattt cctttcatac catattttta gtaggtagaa catttgttct
     4381 gcctattttt tatcccaaaa gtgcagttga ggtgtttatt tatatcgttt ggagtttatt
     4441 tggagtttta catgtatttt tgataaaata ggtatagaaa ttaggaaagt cgaagaaaag
     4501 gaatgaagaa ggttttaaga tattttttag tatttgtttt tctatttttg atgaatattt
     4561 tcatctttaa gatactagca actctgggat ttcaactgac aatgagtgaa aagagttata
     4621 ttgtaccacc gctgttttcg attatcgttt tatatatgat tgacaaaaga attaggaaga
     4681 aaaagaaata aatatatatt ttaggtagaa cgcttgttct gcctattttc tttattcaaa
     4741 aagtgcagtt gggaggtaga taggcgcatt tggggaggaa gtccagtttt tgtttgagga
     4801 ttggggtaag atagttatta tcagatgagt ttatactctt cgaaaatctc ttcaaaccac
     4861 gtcaacgtcg ccttgccgtg tagatgttac tgacttcgtc agttctatct acaacatcaa
     4921 agccgtgctt tgagcaacct gcggctagtt tcctggtttg atctttgatt ttcattgggt
     4981 attaggaaaa ggagatgaat atgaaatttg ggaaacgtca ctatcgtccg caggtggatc
     5041 agatggactg cggtgtagct tcattagcca tggtttttgg ctactatggt agttactatt
     5101 ctttagctca cttgcgagaa ttggctaaga cgaccatgga tgggacgacg gctttgggct
     5161 tggtcaaggt agcagaggag attggctttg agacgcgagc gatcaaggcg gatatgacgc
     5221 tctttgattt gccagatttg acctttcctt ttgttgccca tgtgcttaag gaagggagat
     5281 tgcttcacta ctatgtggtg actgggcagg ataaggacag cattcatatt gctgatccag
     5341 atcctggggt gaaattgacc aaactaccac gtgagcgttt tgcggaagaa tggacaggag
     5401 tgactctgtt tatggcgcct agtccagact ataagcctca taaggatcaa aaaaatggtc
     5461 tgctatcttt tatccctata ttagtgaaac agcgtggttt gattgccaat atcgttttgg
     5521 caacactctt ggtaaccttg attaacattg tgggttctta ttatctgcag tctatcattg
     5581 atacctatgt gccagatcag atgcgttcga cactggggat tatttctatt gggctggtga
     5641 ttgtctatat cctccagcaa atcttgtctt acgctcagga gtatctcttg cttgttttgg
     5701 ggcaacgctt gtctattgat gtgattttgt cctatatcaa gcatgttttt cacctgccta
     5761 tgtccttttt tgcgacacgt cggacagggg aaatcgtgtc tcgttttaca gatgctaaca
     5821 gtatcattga tgcgctggct tcgaccattc tttcgatttt cctagatgtg tcaacggttg
     5881 tcattatttc ccttgtttta ttttcacaaa ataccaatct ctttttcatg actttattgg
     5941 cgcttcctat ctatacagtg attatctttg cctttatgaa gccgtttgaa aagatgaatc
     6001 gggataccat ggaagctaat gcggttctgt cttcttctat cattgaggac atcaatggta
     6061 ttgagactat caaatccttg accagtgaaa gtcagcgtta ccaaaaaatt gacaaggaat
     6121 ttgtggatta tctgaagaaa tcctttacct atagtcgggc agagagtcag caaaaggctc
     6181 tgaaaaaagt tgcccatctc ttgctcaatg tcggtattct ctggatgggg gctgttctgg
     6241 tcatggatgg caagatgagt ttggggcagt tgattaccta taataccttg cttgtttact
     6301 ttaccaatcc tttggaaaat attatcaatc tgcaaaccaa gcttcagaca gcgcaggttg
     6361 ctaataaccg cctaaatgag gtttatctgg tagcttctga gtttgaggag aagaaaacag
     6421 ttgaggatct gagcttgatg aagggagata tgatcttcaa gcaggtttac tataagtatg
     6481 gctatggtcg agatgtcttg tcggatatca atttgactat tcctcaaggc tctaaggtgg
     6541 cttttgtggg gatttcaggg tcaggtaaga cgaccttggc taagatgatg gttaattttt
     6601 acgacccaag tcagggagag attagtctgg gtggtgtcaa tctcaatcaa attgataaaa
     6661 aggctctgcg tcagtatatc aactatctgc ctcaacagcc ctatgtcttt aacggaacga
     6721 ttttggagaa tcttcttttg ggagccaagg aggggacgac ccaggaggat atcttacggg
     6781 cggtcgaatt ggcagagatt cgggaggata ttgagcgcat gccactgaat taccagacag
     6841 aattgacttc ggatggggca ggaatttcag gtggtcaacg gcagagaatc gctctggcgc
     6901 gtgctctctt gacagatgca cctgtcttga ttttggatga ggcgaccagc agtttggata
     6961 ttctgacaga gaagcgaatt gtcgataatc tgatgtcttt agataaaacc ttgattttca
     7021 ttgctcaccg cttgaccatt gctgagcgga cagagaaggt tgttgtcttg gatcagggca
     7081 agattgtcga agaaggaaag catgctgatt tgcttgcaca gggtggcttt tacgcccatt
     7141 tggtgaatag ctagaaagag gagaggatga aagcagaatt tttagaaagt gcggagtttt
     7201 ataatcgtcg ttaccataat ttttccagtc gggtgattgt ccccatgtcc cttctgctcg
     7261 tgtttttgct tggatttgca acatttgcag agaaggagat gagtttgtct actagagcta
     7321 ctgtcgagcc tagtcgtatc cttgccaata tccagtcaac tagcaacaat cgcattcttg
     7381 tcaatcattt ggaagaaaat aagctggtta aaaagggaga gctgttggtt caatatcaag
     7441 aaggggcaga gggtgtccaa gcggaggcct attctagtca gttggacatg ctcaagaatc
     7501 aaaaaaagca attggagtat ttgcaaaaga gcctgcaaga aggggagaac cactttccag
     7561 aggaggataa gtttggctac caagccacct ttcgcgacta cctcagccaa gcggctagtc
     7621 ttagggctag tacatcgcaa caaaatgaga ccatcgcatc tcagaatgca gcagctagcc
     7681 aaacccaagc cgaaatcggc aacctcatca gccaaacaga ggctaaaatt cgcgattacc
     7741 agacagctaa gtcagctatt gaaacaggtg cttccttggc cagtcagaat ctagtctact
     7801 ctctctacca gtcctataag tctcagagtg aggaaaattc ccaagctaaa tcccaggcag
     7861 tggcgcaggt tgaagcgcag ctttctcagt tagaatctag tcttgctact taccgtgttc
     7921 agtatgcagg ttcaggtacc cagcaagcct atgcgtcagg cttaagcagt caattggagt
     7981 ctctcaaatc ccaacacttg gctaaggttg gtcaggaatt gacccttcta gaccagaaaa
     8041 tcttggagac agagtcaggc aagaaggtac agggaaatct tttagacaag gggaaaatta
     8101 cggcgagtga ggatggggtg cttcatctta atactgagac cagtgattct atcatggtag
     8161 cagaaggtgc cctactagcc caactctatc cgtctttgga aaaagaaggg aaagccaaac
     8221 tcacagctta tctaagttcc aaagatgtag caagaatcaa ggtcggtgat tctgttcgct
     8281 atacgacgac tcatgatgcc aagaatcaac ttttcttaga ttctactatt acaagtattg
     8341 atgcgacagc tactaagact gagaaaggga atttctttaa aatcgaggcg gagactaatc
     8401 taacttctga gcaggctgaa aaacttcggt acggggtgga gggtcgtctg cagatgatta
     8461 caggcaagaa aagttatctg cgctattatt tggatcaatt tttgaacaaa gagtaatgtt
     8521 cgtgttttta gagttatact caatgaaaat caaagagcaa actagaaaac tagccgcagg
     8581 ctgtacttga gtacggcaag gcgacgttga cgcagtttga atttgatttt cgaagagtat
     8641 taaatgattt taaaactgtg agaaagatac ttcttgcagt tttttcttta ttatttttga
     8701 agtaaatgtg ttatttattc ggttaaattc ttgtaatttt agggtttttg tggtagaatg
     8761 tgctcaagta atacgaaagg cgaactttaa aatgtcaaaa caattaatct attcgggaaa
     8821 agctaaagat atctatacaa ctgaggatga aaatcttatt atttcgactt acaaggacca
     8881 ggcgactgct tttaacggtg tcaagaagga gcagattgca ggtaagggag tgttgaataa
     8941 tcagatttca tcttttattt ttgagaaatt aaatgcggtt ggtgtggcga ctcactttgt
     9001 ggagaaactt tcagacacag aacaactcaa taaaaaggtt aagattattc ctttggaagt
     9061 cgtgctccgc aactatactg ctggttcctt ttcaaaacgt tttggtgtgg atgagggaat
     9121 cgccttggat actccgattg tcgaatttta ctataaaaat gatgatttgg atgatccatt
     9181 catcaatgac gagcatgtga aattcctaca gattgcggat gaccagcaga ttgcctactt
     9241 gaaggaagaa acccgtcgta tcaatgaact tttgaaggtc tggtttgctg agattgggct
     9301 taagttgatt gactttaagc tagagttcgg ttttgacaag gatggcaaga ttattttggc
     9361 agatgaattt tcaccagata actgccgctt gtgggatgcg gatggcaacc acatggataa
     9421 ggatgttttc cgtagaggat tgggagaact aaccgacgtt tatgagattg tttgggaaaa
     9481 gttgcagggt ttgaaataat ctgttagcaa cggagaacct tcgtctctca actaaaagga
     9541 ctcaggctga aaaggtcccc cagacctttt cactccgtag gggtctaggt gaactaacag
     9601 acgtttacga gattgtctgg gaaaagttgc aggaattgaa ataacaacct caaggctgtt
     9661 tgggaacatt gcaagagctg aaataaagga ataagaattg atggataaac gtatttttgt
     9721 tgaaaaaaag gctgattttc aggtcaagtc agagagtttg gtaagagagc tccagcacaa
     9781 cttggggctt tcaagcttga aaagtattcg tattgtgcaa gtgtatgatg tatttgattt
     9841 ggcagaggac ctgtttgcac ctgcagagaa acacatcttc tctgagcagg tgacagatca
     9901 tgtcttggat gaagcggctg tgcaagcgga tcttgccaac tatgctttct ttgctattga
     9961 aagcctgcca gggcagtttg accagcgtgc agcttcttca caggaagcct tgcttttgct
    10021 gggaagttcg agtgatgtga cagtcaatac agcccaactt tacttggtga ataaagatat
    10081 tgatgcgact gagttagagt cggtcaagaa ctacttgctc aatccagttg attctcgttt
    10141 caaggatatc acgacaggga ttgccaagca ggaattttca gagtcagaca agaccattcc
    10201 gaaattgact ttctttgaaa gctatacagc agaagacttt gcccgctaca aggcagagca
    10261 agggatggca atggaagtgg atgatttgct ctttatccaa gactacttta agtcaatcgg
    10321 gcgcgtgccg acggaaacag aactcaaggt tttggacact tactggtctg atcactgccg
    10381 tcacacgact tttgagacag agttgaaaca cattgatttc tcagcttcta aattccaaaa
    10441 gcaattgcag tcaacctatg aaaaatatat tgccatgcgt gatgagttgg ggcgttctga
    10501 aaagccacaa accttgatgg atatggcgac tattttcggt cgttatgagc gtgccaatgg
    10561 acgtttggac gatatggaag tgtcagatga aatcaatgct tgctcagtcg aaattgaagt
    10621 ggacgttgat ggtgtgaaag agccatggct cctcatgttc aagaacgaaa cccacaacca
    10681 cccaacagaa atcgaaccat ttggtggggc tgctacttgt attggtggag ccattcgtga
    10741 cccattgtca ggtcgctcat atgtctacca agccatgcgt atttcaggtg ctggtgatat
    10801 tacagcaccg atttcggaaa ctcgcgctgg gaaattgcca caacaagtca tttctaaaac
    10861 agctgctcac ggttattctt catatggtaa ccagattggg cttgcaacga cctacgttcg
    10921 tgagtatttt catccaggct ttgtagctaa acgcatggag ctaggtgctg ttgtcggtgc
    10981 ggctcctaag ggcaatgtgg tccgtgaaaa accggaagct ggtgatgtca ttatcctcct
    11041 tggaggtaag actggacgtg atggtgtcgg tggtgcgacg ggctcttcta aggttcaaac
    11101 agttgagtct gtggaaactg ctggagctga ggttcaaaaa ggaaatgcca tcgaagaacg
    11161 caagattcaa cgacttttcc gtaatggcga tgtgactcgt cttatcaaaa aatcaaatga
    11221 ttttggcgct ggtggtgtct gtgtagctat tggtgaatta gcagacggtc ttgaaatcga
    11281 ccttaacaag gtgcctctta aataccaagg cttgaatggt accgaaattg ccatctctga
    11341 gtctcaagaa cggatggctg tggtggttcg tcctgaagat gtggctgcct tcgttgcagc
    11401 atgtaacaaa gaaaatattg atgctgttgt ggtagcgaca gtcactgaaa aaccaaattt
    11461 ggtcatgcac tggaatggtg aaaccattgt cgacttggaa cgtcgtttcc ttgatacaaa
    11521 cggtgtgcgc gtggtcgtcg atgctaaggt tgtggacaag gatgtcaaac tcccagaaga
    11581 acgtcaaaca tctgctgaca cacttgaagt tgataccctt gcggttctat ctgacctcaa
    11641 ccatgcgagt caaaaaggct tgcagactat ctttgattgc tctgttggtc gttcaacggt
    11701 taatcaccca cttggtggtc gttaccaact cacaccaact gaggcttctg tgcagaaatt
    11761 gcctgtacaa catggtgtga ctcatactgc gtcagtcatt gctcaaggtt tcaaccctta
    11821 tgtagcagaa tggtctccat accacggtgc tgcttatgcg gttattgaag cgactgctcg
    11881 tttggtggct gctggtgcca actggtctaa ggctcgtttc tcttaccaag agtatttcga
    11941 gcgcatggac aaacaagcag agcgtttcgg tcagccagta gctgctcttc taggttctat
    12001 cgaagcacaa attcagcttg gcttgccatc tatcggtggt aaggactcta tgtctggtac
    12061 ctttgaagaa ttaaccgtac caccaacctt ggtcgctttt ggggtgacga cagcggatag
    12121 ccgtaaggtg ctttctcccg agttcaaagc tgct
//
