LOCUS       NZ_NBUK01000063         8725 bp    DNA     linear   CON 19-MAR-2024
DEFINITION  Sinorhizobium medicae strain Str8 scaffold51.1, whole genome shotgun
            sequence.
ACCESSION   NZ_NBUK01000063
VERSION     NZ_NBUK01000063.1
KEYWORDS    WGS; RefSeq.
SOURCE      Sinorhizobium medicae (Ensifer medicae)
  ORGANISM  Sinorhizobium medicae
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
REFERENCE   1  (bases 1 to 8725)
  AUTHORS   Porter,S.S., Faber-Hammond,J.J. and Friesen,M.L.
  TITLE     Co-invading symbiotic mutualists of Medicago polymorpha retain high
            ancestral diversity and contain diverse accessory genomes
  JOURNAL   FEMS Microbiol Ecol 94 (1) (2018)
   PUBMED   29228264
REFERENCE   2  (bases 1 to 8725)
  AUTHORS   Porter,S., Friesen,M.L. and Faber-Hammond,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2017) Biology, Washington State University
            Vancouver, 14204 NE Salmon Creek Ave., Vancouver, WA 98686, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: AllPaths v. May-18-2012 Linux x64 A5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: GCA_000017145.1
            Genome Coverage                   :: 33.6x
            Sequencing Technology             :: Illumina Genome Analyzer IIx
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002865145.1-RS_2024_03_19
            Annotation Date                   :: 03/19/2024 04:22:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,364
            CDSs (total)                      :: 6,308
            Genes (coding)                    :: 5,904
            CDSs (with protein)               :: 5,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 404
            CDSs (without protein)            :: 404
            Pseudo Genes (ambiguous residues) :: 0 of 404
            Pseudo Genes (frameshifted)       :: 278 of 404
            Pseudo Genes (incomplete)         :: 206 of 404
            Pseudo Genes (internal stop)      :: 43 of 404
            Pseudo Genes (multiple problems)  :: 106 of 404
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:52:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 8725
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NBUK01000063.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8725
                     /collection_date="2009"
                     /country="USA: California"
                     /db_xref="taxon:110321"
                     /host="Medicago polymorpha"
                     /lat_lon="38.871 N 122.419 W"
                     /mol_type="genomic DNA"
                     /organism="Sinorhizobium medicae"
                     /strain="Str8"
                     /submitter_seqid="scaffold51.1"
     region          1..8725
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-ii"
                     /region_number="1"
                     /rules="cds(LANC_like and DUF4135)"
                     /tool="antismash"
     cand_cluster    1..8725
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(LANC_like and DUF4135)"
                     /kind="single"
                     /product="lanthipeptide-class-ii"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..8725
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[531:3453](-)"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(532..3453)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
     gene            complement(532..3453)
                     /locus_tag="BMJ25_RS30185"
                     /old_locus_tag="BMJ25_29930"
     CDS             complement(532..3453)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070: lanthionine synthetase C family protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011970831.1"
                     /locus_tag="BMJ25_RS30185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BMJ25_29930"
                     /product="type 2 lanthipeptide synthetase LanM family
                     protein"
                     /protein_id="WP_011970831.1"
                     /sec_met_domain="DUF4135 (E-value: 2.8e-78, bitscore:
                     252.1, seeds: 79, tool: rule-based-clusters)"
                     /sec_met_domain="LANC_like (E-value: 1.7e-13, bitscore:
                     38.5, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIDANIRKEARDNTAIVALWPLVDELADPVGNTGAPREKAGSEDA
                     FLSGLCFAARQDISRTPCLAKELPFVHLWFPLAEVAMGLLPQAALAPTVIGQLIVALTE
                     RLCALGADALQQILQERRGAGASVIAALAPEKDPSAAKCEIYDRLTDELRQTHLAELLD
                     RFPVLRRLIPFTIAVWIRNLRELLARLEADRTAIAQNFRLPEDAALSGMQFAVGDTHKG
                     GRSVVLLEFTWQGRQTKLVYKPRNLALEAAFQNLLADPRASIGLSPLPGLKIWCAEDYG
                     YMEFVEGAPCDNEDVLQAFYRSAGRLAALLHVLGYTDGHHENLVAHHSHLYVIDAETLL
                     TPFEKPAIRRPRPGDSLGAAQTAWSWFDTTVARTELFPAWTLLGLARDAVDTSAFGIDP
                     QEWPRQIPGWHSINTDNMARTVMIGELVRTQSLPVTSDRKNPFFRHRKLFMAGFHAQCR
                     SIIANKPVWLGEDGLLQAFNGCKGRAVIRATRIYAALARRQLTAEALRSEQSQADVLYP
                     LERLFAPLADRQDVSSLCASEKLQMRQLDIPVFTHTVSQTALNLTGDQRIDDYFETSGL
                     AASRERIEALSCERIEREIETIDALASAKALRLAQHGRSCENSLQPITNPTDQLLSEIR
                     RRSDGWIGFDLAPDGIRFCYQPLGMSLVSGTLGLAVYLAATGRPEACHIAENLAEPLLR
                     LAESGTSAERLRWWRDLPIGLRGSGGQLLALSALQRLSIMADRIPHAMQQLVAVLDRAL
                     LEDKPWDVWSGYAGLLGPLLLVNSDTADTQAEMVACRLADWTSREAETAPSGFAQGAFG
                     VLAALSQFAHASGNANIRASVQSLLQHSSEAFWRFGPRAGSEWSCGIAGHIITCLCLRH
                     DQEWRANADAAIRRGVIGLASLPNQGAGIHSGTAGVEMACASSAQATGNLPCSSDTQKY
                     CDPRHLGLFTGVAGIGFAQIRDERSNAARDLIISAGLLPTIGH"
     gene            3639..4745
                     /locus_tag="BMJ25_RS30190"
                     /old_locus_tag="BMJ25_29935"
     CDS             3639..4745
                     /GO_function="GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017266285.1"
                     /locus_tag="BMJ25_RS30190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BMJ25_29935"
                     /product="C1 family peptidase"
                     /protein_id="WP_234705535.1"
                     /transl_table=11
                     /translation="MPGPQRQERTLPTINRYGGPDGGYVAVCSRVADHAVYSVGDGIYV
                     VGQIRLQGAYEGRFFVPKGYGGKCISAAPDIKAICDQAFPGSAPNWASGDAGGWFGLLI
                     DKRDVRDKILTLDLPQQLPEKVDLRRWCSQVQNQGTLNACTAYAASAILEYFENRAGGN
                     AVSLSEIFLYKVTRNLMHKTGDTGANTRSVMKALAALGTVPEEYWPDDASQFDAEPSAF
                     AYALAGRYRSLKYSRIDAKDRSKDVVLRQAKTLLSRNRPVMFGVMAYFGTWQQFVTSDR
                     LPYPSEDDTLFGAHNIAVMGYDDGITTENAKNPGIKTRGAFLIKNSYGEEWGDKGYGWI
                     PYDYLLKHQSIDWWTITKQEWLDMSVFS"
     gene            5140..>5734
                     /locus_tag="BMJ25_RS30195"
                     /pseudo=""
     CDS             5140..>5734
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003563912.1"
                     /locus_tag="BMJ25_RS30195"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MGLDVH"
     gene            5828..6012
                     /locus_tag="BMJ25_RS35545"
                     /pseudo=""
     CDS             5828..6012
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017276878.1"
                     /locus_tag="BMJ25_RS35545"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MEGRQRDIAAYLPAQSGISEHELLQKLIRRLQAARRRMRLATIGA
                     AGGPTKIPTMVAMIPP"
     gene            6172..6648
                     /locus_tag="BMJ25_RS34260"
                     /old_locus_tag="BMJ25_29945"
     CDS             6172..6648
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011970829.1"
                     /locus_tag="BMJ25_RS34260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BMJ25_29945"
                     /product="thermonuclease family protein"
                     /protein_id="WP_101806913.1"
                     /transl_table=11
                     /translation="MLKNSSCCSPVLALLLSASLATAAETITGRSRVIDGDTIEIRGER
                     IRLHGVDAPESWRGCEDGDGGTYRCGKEAAYALDSFVAPSRPIRCELVERTRYKRRVSI
                     CFRADGREVNRWLVESGNAADWPCCGKAGHLARKVRPAVRGARAVRAKGNASSC"
     CDS             6661..6894
                     /gene="allorf_6661_6894"
                     /locus_tag="allorf_6661_6894"
                     /protein_id="allorf_6661_6894"
                     /tool="antismash"
                     /transl_table=1
                     /translation="MMVVYLRASPSAAYQRINSCQPIRDNESAISYLSEVAERLNKQLI
                     KVQGENMVTKSLIKRLMMLVCPPSRRVPPGLG"
     CDS_motif       6661..6753
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MMVVYLRASPSAAYQRINSCQPIRDNESAIS"
                     /locus_tag="allorf_6661_6894_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       6754..6894
                     /prepeptide="core"
                     /locus_tag="allorf_6661_6894_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-7.30"
                     /molecular_weight="5122.2"
                     /monoisotopic_mass="5118.9"
                     /core_sequence="YLSEVAERLNKQLIKVQGENMVTKSLIKRLMMLVCPPSRRVPP
                     GLG"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MMVVYLRASPSAAYQRINSCQPIRDNESAIS"
                     /leader_location="[6660:6753](+)"
                     /alternative_weights="5140.2"
                     /alternative_weights="5158.2"
                     /alternative_weights="5176.2"
     misc_feature    6695..6715
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 25.34"
                     /tool="antismash"
     CDS             7325..7396
                     /gene="allorf_7325_7396"
                     /locus_tag="allorf_7325_7396"
                     /protein_id="allorf_7325_7396"
                     /tool="antismash"
                     /transl_table=1
                     /translation="MVASAGGFPAPARSAEPADVHNC"
     CDS_motif       7325..7345
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MVASAGG"
                     /locus_tag="allorf_7325_7396_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       7346..7396
                     /prepeptide="core"
                     /locus_tag="allorf_7325_7396_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-5.40"
                     /molecular_weight="1663.8"
                     /monoisotopic_mass="1662.8"
                     /core_sequence="FPAPARSAEPADVHNC"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MVASAGG"
                     /leader_location="[7324:7345](+)"
     misc_feature    complement(7382..7397)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 21.62"
                     /tool="antismash"
     CDS             complement(7639..7821)
                     /gene="allorf_7639_7821"
                     /locus_tag="allorf_7639_7821"
                     /protein_id="allorf_7639_7821"
                     /tool="antismash"
                     /transl_table=1
                     /translation="MLNCAQRAFSKAPLPTGVIWRCSVIPTLRVVGTIPGAPSAAATAS
                     IPVDTWPFSTKDSDC"
     CDS_motif       complement(7711..7821)
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence:
                     MLNCAQRAFSKAPLPTGVIWRCSVIPTLRVVGTIPGA"
                     /locus_tag="allorf_7639_7821_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       complement(7639..7710)
                     /prepeptide="core"
                     /locus_tag="allorf_7639_7821_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-4.20"
                     /molecular_weight="2241.4"
                     /monoisotopic_mass="2240.1"
                     /core_sequence="PSAAATASIPVDTWPFSTKDSDC"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MLNCAQRAFSKAPLPTGVIWRCSVIPTLRVVGTIPGA"
                     /leader_location="[7710:7821](-)"
                     /alternative_weights="2259.4"
                     /alternative_weights="2277.4"
                     /alternative_weights="2295.5"
                     /alternative_weights="2313.5"
                     /alternative_weights="2331.5"
                     /alternative_weights="2349.5"
     CDS             7824..7970
                     /gene="allorf_7824_7970"
                     /locus_tag="allorf_7824_7970"
                     /protein_id="allorf_7824_7970"
                     /tool="antismash"
                     /transl_table=1
                     /translation="MQPTSLGGPRPLHFILVDCAPVANPTYVEADLRATMKHDQFMCFA
                     IHV"
     CDS_motif       7824..7847
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MQPTSLGG"
                     /locus_tag="allorf_7824_7970_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       7848..7970
                     /prepeptide="core"
                     /locus_tag="allorf_7824_7970_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-5.30"
                     /molecular_weight="4532.3"
                     /monoisotopic_mass="4529.3"
                     /core_sequence="PRPLHFILVDCAPVANPTYVEADLRATMKHDQFMCFAIHV"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MQPTSLGG"
                     /leader_location="[7823:7847](+)"
     misc_feature    7877..7893
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 25.58"
                     /tool="antismash"
     CDS             7954..8058
                     /gene="allorf_7954_8058"
                     /locus_tag="allorf_7954_8058"
                     /protein_id="allorf_7954_8058"
                     /tool="antismash"
                     /transl_table=1
                     /translation="MRYMYEATYARLCPSSSTISKIYLKQITGSNCTI"
     CDS_motif       7954..8043
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MRYMYEATYARLCPSSSTISKIYLKQITGS"
                     /locus_tag="allorf_7954_8058_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       8044..8058
                     /prepeptide="core"
                     /locus_tag="allorf_7954_8058_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-4.20"
                     /molecular_weight="431.5"
                     /monoisotopic_mass="431.2"
                     /core_sequence="NCTI"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MRYMYEATYARLCPSSSTISKIYLKQITGS"
                     /leader_location="[7953:8043](+)"
ORIGIN
        1 ccagcaaacc gtccttgccg aaaacctcgg atggatcacg tccagccagg agctggtcca
       61 gaagctcttt ctcgatagcc atgtgatgat tcgctccttt tcatcatcat ggcccaccgc
      121 acagaattcc tgacagtccc gtagcattcg gtagccgcgc tggtacataa ccgggcctct
      181 tacttgcagg tttcagatta tgggggtgga tgtgcgttta agggtcacag tgccggccac
      241 gactagaacg acttcgatgg cgacagtcgc aattacggtg gtgccgttcg attgattttg
      301 gccggagatc tccccttcaa caaatgcgtc cgagcgtgct gtgcaattgt tggcgtcatc
      361 gatcgtgaaa tggtagccac gcttcctgac gctcgccacc agcgttgcga agccccatag
      421 tatttgattt aacatgttcg acgaggacaa aagcaaaaac gatcatatca aaacaaattt
      481 tcaaaacaca gcctttcggc gtttgacaaa aaagcttgta cttctgaccg ttcaatggcc
      541 tatggtcgga agaagaccag cagagatgat aaggtcgcga gccgcgttgg accgctcgtc
      601 ccggatctgt gcgaacccta taccagcaac gccagtgaac agaccgagat ggcgtggatc
      661 gcagtacttc tgggtgtctg aagagcatgg caaattgcca gttgcctgtg ctgatgacgc
      721 gcaggccatc tcgacgccgg cggtgccgct gtggataccg gctccttggt taggcaggga
      781 cgccaatccg atcaccccgc gccggattgc agcatccgca ttcgctcgcc attcttggtc
      841 atgacgcagg cacaggcatg tgatgatgtg acctgcaatg ccgcaactcc attcagaacc
      901 ggcgcgaggg ccgaagcgcc agaaggcttc cgagctgtgt tgaagcaggg attgaaccga
      961 agcgcgaatg tttgcgttcc ctgatgcatg cgcaaactga ctgagcgcag ccaatacacc
     1021 aaaagcacct tgcgcaaagc cagacggtgc cgtttccgcc tctctgctgg tccagtcggc
     1081 cagacggcag gcaaccatct cggcctgagt gtccgctgtg tcggaattca cgagtaggag
     1141 cggacccagc aagcccgcat atccggacca gacatcccaa ggcttatctt cgagcaaggc
     1201 ccgatcgaga actgcaacca actgctgcat ggcgtgcggg attcgatcgg ccattatgct
     1261 aagcctctgc agtgccgaca atgccagaag ctgtccgccg ctgccacgca acccgatggg
     1321 caggtctcgc caccaccgca ggcgctcagc cgatgttccg ctctccgcca gcctcagaag
     1381 cggctcagcc aaattttctg caatatggca ggcctctggt cttccggtcg ctgcaagata
     1441 gacggcaaga ccaagtgtgc cgctcacgag cgacattcca agcggctggt agcagaaccg
     1501 gataccatcc ggcgcgagat cgaatccgat ccagccatct gatcgacgcc tgatttccga
     1561 taaaagctgg tctgtcggat tggtgatcgg ttggaggctg ttctcgcaac tccgcccgtg
     1621 ctgcgcgagc cgcaatgcct tggccgatgc caaagcgtct attgtttcga tctcacgctc
     1681 gatcctttcg caggacaatg cctctattcg ctcccggctt gccgccaagc cactcgtctc
     1741 gaaatagtca tcgattcgtt gatcgccggt caggttcagg gccgtttgcg agaccgtgtg
     1801 cgtgaatacg ggaatatcca gttggcgcat ttgcagcttt tcagaggcgc ataggcttga
     1861 aacgtcctga cgatctgcaa gcggcgcaaa aaggcgctcc agcggataaa gaacgtctgc
     1921 ttgggactgc tcgcttcgca atgcttccgc cgtcaactga cggcgtgcaa gcgccgcata
     1981 gatccgtgtc gcgcgaatga cagcgcgccc cttgcagccg ttgaaggcct gcaacaggcc
     2041 atcttccccc agccaaacgg gtttgttggc gatgatggac ctgcactgcg catggaatcc
     2101 ggccatgaag agcttacgat gacggaaaaa tggattcttc ctgtctgacg ttacgggcaa
     2161 tgattgcgtg cgaacgagct ccccgatcat gacggtgcgg gccatgttat ccgtattgat
     2221 ggagtgccag ccgggaattt gccttggcca ttcctgagga tcgatgccga aagcgctcgt
     2281 atcgacggca tcgcgtgcga ggcccagtaa ggtccaggct ggaaatagct cggttcgtgc
     2341 aacggtggtg tcgaaccaag accatgccgt ctgggccgca cccaggctgt cccccggccg
     2401 tggacggcga attgctggct tctcgaaggg cgtcaaaagc gtttcggcgt ctataacata
     2461 tagatggctg tgatgcgcca cgaggttttc gtggtgacca tccgtgtaac ctaaaacatg
     2521 caggagagcg gccagtctgc cggcactgcg atagaatgcc tgcagtacat cttcgttgtc
     2581 gcagggcgcg ccctcaacaa attccatata gccatagtct tctgcgcacc agattttcag
     2641 cccgggaaga ggtgaaagac cgatgctagc gcgcggatcc gccagcaaat tctggaacgc
     2701 cgcctcgagg gcgaggttac ggggcttgta gacgagtttt gtctgccgcc cctgccaagt
     2761 gaattccagc agcacgacgc tccgtccacc cttatgggta tcgccgacag caaattgcat
     2821 gccggaaagc gccgcgtctt caggaaggcg gaagttttga gcgattgctg tccgatccgc
     2881 ctcgaggcgg gctaaaagct cgcgaagatt tctgatccag actgcaattg tgaaaggaat
     2941 caagcgtcga aggaccggaa agcgatccag cagttccgcc agatgtgtct gccgcagttc
     3001 gtctgtcaag cgatcataga tctcgcattt cgcggcgctc gggtctttct ccggggctaa
     3061 tgcggcgatg acggacgcgc cagcgccacg ccgctcttga agtatttgct gaagtgcatc
     3121 tgcccccaac gcgcaaaggc gctctgtcag cgctacaatc agttgcccga tgacggttgg
     3181 cgccaacgcg gcttgcggca gaagccccat tgcgacttcg gccagaggaa accacaggtg
     3241 aacaaaaggc agctctttcg caaggcaagg cgttcgcgaa atgtcttgcc tcgccgcgaa
     3301 gcataaaccc gagagaaacg catcttcgga accggctttc tcacgaggcg cgcctgtatt
     3361 gcccaccgga tcggcaagct cgtccaccaa tggccaaagg gctactatcg ccgtattgtc
     3421 tcgtgcctct tttcggatgt ttgcgtcgat catggcgatg cctcccgcac aaatctccct
     3481 ttcgatccgg caacatttcc gcattgacag ctatggatat tgcgacatgg tgcaatctga
     3541 ggaatgtgga gacatatatg cagcctacaa tgccgttcta taactttctg ccggaggatt
     3601 tcaaggtcta tgtcggcgcg gatggaggtg tgtcttacat gcccggcccg cagcggcagg
     3661 agcgcacttt gccgacgatc aatcgttacg gggggcctga cggtggctat gtcgcggtct
     3721 gttcgcgtgt ggcggatcat gctgtctatt ccgttggcga cgggatctat gtggttggcc
     3781 agatcaggct gcaaggtgcc tatgaggggc gctttttcgt gccgaagggc tatggaggaa
     3841 agtgcatcag cgctgctccg gacataaagg cgatatgcga tcaagccttt cccggtagcg
     3901 cacccaactg ggcgagcggc gatgccggcg gttggtttgg tctgctgatc gataagcggg
     3961 acgttcgcga taagatcctc acgttggatt tgccccagca attgccggaa aaggttgatt
     4021 tgcgacgatg gtgctcgcag gtccaaaatc agggcacctt gaacgcctgc accgcctacg
     4081 ccgcctcggc catcctggaa tatttcgaaa atcgcgccgg cggaaacgca gtgtcgctgt
     4141 cggaaatttt cctttacaag gtcacgcgca acctgatgca caagacgggc gacacgggag
     4201 ccaatacgcg atccgtcatg aaggctttgg cagccttagg cacggtgccg gaagaatatt
     4261 ggccggatga cgcatcccaa ttcgatgctg aaccgtctgc ctttgcctat gcgctggcag
     4321 gtcgttatcg ctctctcaag tacagcagga ttgatgcgaa ggaccgttca aaggacgtgg
     4381 tgttgcgcca ggccaagaca ttgctcagtc ggaaccgtcc ggtcatgttt ggcgtcatgg
     4441 cgtatttcgg cacttggcag caattcgtga cgtccgatcg cctgccttac ccgagtgagg
     4501 atgacacgct ttttggcgcc cataacattg ccgtcatggg ctatgacgat ggcatcacaa
     4561 cggaaaatgc caagaacccc ggcatcaaga cgaggggcgc cttcctcatt aaaaactcct
     4621 atggcgagga gtggggtgac aagggctacg gctggattcc ttacgattac ctactgaaac
     4681 atcagtcgat tgactggtgg acgatcacca agcaggaatg gctcgacatg agcgtgttca
     4741 gctaaatgaa aggtataaaa tgcaaacccg tttccgcgtg accgcggaaa cgggttcttc
     4801 tcctgacttt tcgttagccg agtcatcatg gtagcctcct cgatgctggt tccgctgatc
     4861 ggtcgaaatc cgccgactta aaaccactaa ggagaaagat gttcgggcgt acgcgaggga
     4921 ccttaagcga aggcatgatg cggcggatgc gttttgttgg cgttctcgac tttctgccac
     4981 gttgaatacg aatcgcagag catagggtaa tcgcggtgcg atcgtccaaa gccttttcgt
     5041 aagatgatgc tgacggggtg gtcgcccccg atgagatgga aaggcgggct cacgattgga
     5101 accggctaat atcaagcggc agcaacccgg gaccaatata ttgggcttga tgttcattga
     5161 aagatacggc gacctcggtt cgggagggcg ggaagcggat ctggcgggga aagtgccttt
     5221 cggacccaaa gctcggggct gacattaccc gcaagcacgc gccgcacgcc aggcgcgtcg
     5281 tctttgaaaa cggggccgct gtcgacgtgg ttctatcatg cgctaatggc cgaaggggct
     5341 ccggcgatct gcattgaagc gcggcacgcg cataaggtat tgagcgaaac actcaacaag
     5401 accgacgcca atgatgccga tggcctggct cagctcgctg aagtcggctt tttataaaac
     5461 ggttcgcgtc acgtcgttcg acgctatgat ggcccacgcg ttggggcggc cgcaatctgc
     5521 tgctgagcct ctcaagccaa cttggcaatc agatccgcgg gtcatgaaga cgttcggcct
     5581 tatcgtgcgc aacggcacag gtcggatttt tgatcccaat gttcgagagc ttctggagaa
     5641 gcagactact ttggtacaga tcattttgcc cttacttgat gcgtggcgcg atcttcgcaa
     5701 gcgtgcggcc attcttgaac gtcagctcat cgcagagggc gcggcagagc ccggctacaa
     5761 agatgcttta tatctcccaa gtcctgtatc ttgggacgcg ggtctccaga tttggcctcg
     5821 aggcgcgata gagggtcgcc agcgcgatat agccgcttac ctgccggcgc aaagcggcat
     5881 cagcgagcac gagctgcttc agaagctcat cagacgactt caggccgcca ggcgaagaat
     5941 gcgcttggca acgattggcg cggctggtgg cccgacgaag atcccgacga tggtggcgat
     6001 gataccgcct gatcgaaggt gccgggttgg ccgctgctgt tccgcacggc cccggctggc
     6061 agtcgccagt tgcaatcccc cactcagccg cttctggttt actctaccag ccctcttttg
     6121 actgaaatcg agccttcgac gagcagttag ggaccaaccc agagaggctg catgttgaaa
     6181 aattcgtcct gttgttcacc ggtgttggcg ctgctcttgt cggccagcct cgcgacggct
     6241 gccgaaacca ttaccggccg ctcaagggtg atcgacggag atacaatcga aatcagaggc
     6301 gagcgcatcc gcttgcacgg cgtcgatgct ccggaaagct ggcgaggatg cgaagacggc
     6361 gacggcggta cctatcgctg cggtaaagag gccgcttacg cgctagacag ttttgttgcc
     6421 ccgtcgcgcc ctattcgttg cgagctcgtc gagcgcactc gatacaagcg ccgggtcagc
     6481 atttgctttc gcgccgacgg tcgcgaggtc aatcgctggc tggttgagag tggcaacgcg
     6541 gcagattggc cctgttgcgg caaagcggga cacctggcga ggaaggttcg tcctgccgtg
     6601 cgaggcgcgc gtgcggtccg cgcaaaaggg aacgcatcat catgttgatg ctagcagagc
     6661 atgatggttg tgtatcttcg cgcctccccc agtgcagcat accaaaggat aaattcatgc
     6721 caacctatcc gcgataatga gagcgcaatc agctacctca gcgaggttgc agagaggctc
     6781 aacaagcaac tgattaaggt ccagggcgag aacatggtga cgaagtccct gatcaaacgg
     6841 ctcatgatgc tcgtttgtcc tccctcgcgc cgagttccgc cagggctggg atgaggcact
     6901 tgactcgacc gcggcgattg gataccgaaa ttggaacagg caaatgagcc aacgcgggcg
     6961 ttcgttcaag ctgtgataaa ccttctcgcc tactactgac cacgacccac ggtgttctca
     7021 gggtacttga gggaggcttg cggaattgat gccccccggt tctgccgctt ccacgggcga
     7081 agccgtacag cgcgttttct ctacgtggtg agattcgcaa acaccttgcc gagcgtgccc
     7141 tgagtcgaat ttgggctaaa tagaagcacg tccgacacag ccgagttgct gcggccctcc
     7201 cttttttcgt cgtttccacc ccggcgcaaa actttcgcgc ttcctgacgt cgtcatttgt
     7261 cgtcagccgc gcgcagtggg cgcctaacag caccggactc cgtagcggcc tcgcgcgtgt
     7321 cgaaatggtt gcttctgcgg gtggattccc agcgcccgcg cgcagcgccg agcctgctga
     7381 tgttcacaat tgttgacagt ttcggtgcag gggatcgctt cgactcgact tcaaagcaac
     7441 ttcttgcttg aaatcttccg ccgaaaagca ccgatccatc tacactggcc gcgtgaactt
     7501 gaattatgct cttggccaaa tccaagctga ctatgctaac cttttctatg gacgctctcc
     7561 cttgtaatgg ttccacacaa ccattctggc acgaagatgc cgttgagtgg gagcgtccac
     7621 cccatcgctc acgttgaatc agcagtcgct gtctttggta gaaaaaggcc atgtatcgac
     7681 tggaatgctc gccgttgctg cggccgacgg cgcgccgggt atggtgccta cgactctcag
     7741 ggtagggatg acgctacagc gccagatgac gccggtcggt aagggggcct tactgaacgc
     7801 gcgctgggcg caattcaaca atcatgcagc caaccagttt gggcggtccc cgaccgttgc
     7861 acttcatcct cgtcgactgc gcaccagtcg caaatccgac atatgtcgaa gccgacctcc
     7921 gcgccacgat gaagcacgat cagtttatgt gctttgcgat acatgtatga ggcgacatac
     7981 gcgcgactct gtccttcatc atcaacgatt tcgaagatat acctcaaaca aatcaccggc
     8041 agtaattgta caatctgagg agtattcgcc aacgcttggg ggaggcttgt acccttccgc
     8101 ccaaaggagc gctttccatt ctgccggata cgcggctcct tagcgacgag taacacgcgg
     8161 cttcgcgcct cgatggctta tcagcgtctt gaccgcgcga gacctccctt ggaatttgga
     8221 aaagatatcg cgttcggcat aacgcccccc tcgcaactcg aagctgaagt ctaggagatt
     8281 cccgcttcag agtttcggtt gtgacccctg ccttcatatt gatgatcctt cccttcccgt
     8341 gacctcgcgc catcgcccct gtcctggata caagcctcaa agttccggca accgcacggg
     8401 gcagacgaag atccggctgt ggtgccgctc cccacggccc tgcggcttgg aaggtgacaa
     8461 tccgacagct ggtcgccggg cgggatgacg gccggccccg agacgatcct catgcagaga
     8521 ttcacatcca agaaggtggc cagcagaccg gatgatcagc gtcggatcga cctgtctcaa
     8581 cgagatcgct cgcgtctctt tgttgcgtag caaaaaaggg cggctcgaga gccgcccttc
     8641 agatccgaat ggatcctgcc aaggctgtcg ccttatcaca tcatgtccat gccgcccatg
     8701 ccgcccatgc cgccaggcat tgccg
//
