LOCUS       NZ_NBUK01000052        11215 bp    DNA     linear   CON 19-MAR-2024
DEFINITION  Sinorhizobium medicae strain Str8 scaffold11.1, whole genome shotgun
            sequence.
ACCESSION   NZ_NBUK01000052
VERSION     NZ_NBUK01000052.1
KEYWORDS    WGS; RefSeq.
SOURCE      Sinorhizobium medicae (Ensifer medicae)
  ORGANISM  Sinorhizobium medicae
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
REFERENCE   1  (bases 1 to 215182)
  AUTHORS   Porter,S.S., Faber-Hammond,J.J. and Friesen,M.L.
  TITLE     Co-invading symbiotic mutualists of Medicago polymorpha retain high
            ancestral diversity and contain diverse accessory genomes
  JOURNAL   FEMS Microbiol Ecol 94 (1) (2018)
   PUBMED   29228264
REFERENCE   2  (bases 1 to 215182)
  AUTHORS   Porter,S., Friesen,M.L. and Faber-Hammond,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2017) Biology, Washington State University
            Vancouver, 14204 NE Salmon Creek Ave., Vancouver, WA 98686, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: AllPaths v. May-18-2012 Linux x64 A5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: GCA_000017145.1
            Genome Coverage                   :: 33.6x
            Sequencing Technology             :: Illumina Genome Analyzer IIx
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002865145.1-RS_2024_03_19
            Annotation Date                   :: 03/19/2024 04:22:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,364
            CDSs (total)                      :: 6,308
            Genes (coding)                    :: 5,904
            CDSs (with protein)               :: 5,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 404
            CDSs (without protein)            :: 404
            Pseudo Genes (ambiguous residues) :: 0 of 404
            Pseudo Genes (frameshifted)       :: 278 of 404
            Pseudo Genes (incomplete)         :: 206 of 404
            Pseudo Genes (internal stop)      :: 43 of 404
            Pseudo Genes (multiple problems)  :: 106 of 404
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:52:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 174717
            Orig. end                         :: 185932
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NBUK01000052.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11215
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11215
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11215
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6215](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6215
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    724..739
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 17.55"
                     /tool="antismash"
     misc_feature    complement(896..905)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: medium, score: 16.35"
                     /tool="antismash"
     misc_feature    1062..1077
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.96"
                     /tool="antismash"
     misc_feature    1213..1227
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 19.28"
                     /tool="antismash"
     gene            1459..1790
                     /locus_tag="BMJ25_RS25855"
                     /old_locus_tag="BMJ25_25630"
                     /pseudo=""
     CDS             1459..1790
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007787770.1"
                     /locus_tag="BMJ25_RS25855"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="BMJ25_25630"
                     /product="helix-turn-helix transcriptional regulator"
                     /pseudo=""
                     /transl_table=11
                     /translation="MPRWKKPTKEEVLENRRDLANRARSGELRLPHAVAEIRKGFGMTQ
                     EEFAKMLALTRRQVAEIEAGTANPTLETLEKMAVYSALGWVSCRGR"
     gene            complement(1775..2566)
                     /gene="aac(3)-IV"
                     /locus_tag="BMJ25_RS25860"
                     /old_locus_tag="BMJ25_25635"
     CDS             complement(1775..2566)
                     /codon_start=1
                     /gene="aac_3_-IV"
                     /gene_functions="resistance (resist) AAC3"
                     /gene_kind="resistance"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011976343.1"
                     /locus_tag="BMJ25_RS25860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BMJ25_25635"
                     /product="AAC(3)-IV family aminoglycoside
                     N-acetyltransferase"
                     /protein_id="WP_018208010.1"
                     /transl_table=11
                     /translation="MTQREWRISELVNQLLTLGVKPGGVLVVHSSFRSVRPVEGGPAGM
                     IAALRTAIGPEGTLVMPSWSGLDDEPFEPATTSATSDLGILADTFWRLEGVVRSDHPFA
                     FAAAGPLAARITSDPLPLPPHCPPSPIGRIHELDGQVILLGVGHDSNTTLHLAELMSKV
                     PYGVPRHCTIVENGSRVRIAYLENDHCCQNFSLADVWLRRNGLQKEGQIGNAHARLVRS
                     RDIVKIAMEQLAHDPLVFLHPAEVACEECDAARQSIGYVRS"
     misc_feature    complement(2638..2653)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 19.43"
                     /tool="antismash"
     misc_feature    complement(2736..2752)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.12"
                     /tool="antismash"
     misc_feature    complement(2749..2762)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.78"
                     /tool="antismash"
     gene            2933..4771
                     /locus_tag="BMJ25_RS25865"
                     /old_locus_tag="BMJ25_25640"
     CDS             2933..4771
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1272: TPR
                     repeat-containing protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018095573.1"
                     /locus_tag="BMJ25_RS25865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BMJ25_25640"
                     /product="adenylate cyclase"
                     /protein_id="WP_101777076.1"
                     /transl_table=11
                     /translation="MAVPHFHLQTFGELRLVDSGGVVVACPERGLLILAYLSISGQRTV
                     SREKLSMLIWPEREKSVAFKNLRSTLWRMRHLAANSGPLTSATEIDVTLGEIACDAAGF
                     EALGSSEEAFRAGLSLWRDTFLAGADVPPGRYADWVGEQRSVFTQKLRAALLAGGERFA
                     DRSMLRTACMHLLELDPADTAVRAILERLTGVGLVIGKGSPDGAWLRQGAFAGESPRAP
                     ARDAITPPVAPAPRVALLPPAAFGSDPMLHAVSMAVVEDITIGLCALRSMSVVAPYTAE
                     RIRDAADKAAFLEKHAVTYALDCRMSDQGLFTQLIFLPSDAIVWAERFSISPVGLLQQR
                     QEIAFHVASAVAEQVETGRIGHLDYVTNPDAYYAYLAGLRNLSNVGLPEIRRARRDFKT
                     ALRHKPDFAPALSGIARTYAIEWVLTARGDQELLTVAERHAKEAIESDEELPGAHREFG
                     VVRLYQGDLDGSLAALNRAENLSPHYADVLYSHADTLVHASRPREALDKLGKALSLNPL
                     APDMYFWSAAGASYFLEEYEDAIGYVQKMKDKSPGDRLLAASWAMLGDQKKARSYKVKA
                     LRANPTFDVDKWLAVVPMKEQWQKDLYREGLKRAGF"
     gene            4789..5004
                     /locus_tag="BMJ25_RS25870"
                     /old_locus_tag="BMJ25_25645"
     CDS             4789..5004
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018095572.1"
                     /locus_tag="BMJ25_RS25870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BMJ25_25645"
                     /product="hypothetical protein"
                     /protein_id="WP_162305288.1"
                     /transl_table=11
                     /translation="MAEVVVIGIAGEHGLWLADLKNGTVRPLDQVSGDLATANKLRLQG
                     GTIVKNVDLAVAVSSADKVFSGHFDG"
     gene            5001..6215
                     /locus_tag="BMJ25_RS25875"
                     /old_locus_tag="BMJ25_25650"
     CDS             5001..6215
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011976340.1"
                     /locus_tag="BMJ25_RS25875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BMJ25_25650"
                     /product="YcaO-like family protein"
                     /protein_id="WP_011976340.1"
                     /sec_met_domain="YcaO (E-value: 2e-80, bitscore: 264.7,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIGFAVGSPADTRNAYSDRVLSPEETFDRVRPLLSRFGVTRIARH
                     TGLDRIGLPVWCAYTPNARSIAVAQGKGLTDPEAKVSAVMEALERAVACEPAVGTMVAT
                     ARTLRASGRTVELLPCLIAAGQQDIGPDEELAWALGSDLISGREILVPLAAALLDRTLH
                     SRFWMSSDGLASGNVVEEAILHGLLERIERDAHVLWGISGREHRYGCCVAPESLGDAAL
                     NGLVERIGAAGLDLRLFEITSDIGIPCFLALLGPPDIATRGGGRFVEVTSGCGAHVFPA
                     RAAIRAVTEAAQSRLTFISGARDDVFPETFNRPLPELTRQAFLAEPSRRAIASPQVGGS
                     LEALLRHTVDHLTSAGVQSAIALRLSANALPFSVVKIFVPKLENPDGDRARRFGPRALS
                     KALAS"
     gene            6351..6572
                     /locus_tag="BMJ25_RS25880"
                     /old_locus_tag="BMJ25_25655"
     CDS             6351..6572
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534187.1"
                     /locus_tag="BMJ25_RS25880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BMJ25_25655"
                     /product="hypothetical protein"
                     /protein_id="WP_011976339.1"
                     /transl_table=11
                     /translation="MTNHSSSLTDTEVELASIARMLSHIRESARALEAQAVEYCIDMAL
                     QAVANELRDRGELVIAAAGFDNRTTLLQ"
     gene            complement(6679..7473)
                     /locus_tag="BMJ25_RS25885"
                     /old_locus_tag="BMJ25_25660"
     CDS             complement(6679..7473)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013844664.1"
                     /locus_tag="BMJ25_RS25885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BMJ25_25660"
                     /product="DUF1194 domain-containing protein"
                     /protein_id="WP_018012669.1"
                     /transl_table=11
                     /translation="MLGTMALILALSGAPIQSAAISADVDVELLLAVDMSGSMDMEEAR
                     VQRSGYLQALRHPDFINAVQGGLLGRIAIGYFEWAGLVNEASVVSWQVIESSADAEAFA
                     GKLEARPIGTRRGTSISNAILFGSGLIDSNAYSGTRRVIDISGDGPNNTGPPVTPARND
                     ALSRGIIINGLAILIRPSVSTGPLDRYYAECVIGGPGSFVLPVHEPEDFAVAIRQKLIL
                     EVSGREPDPALRLAAGENAADCMIGEKLRPGFLDRVYPELDR"
     gene            7705..9018
                     /locus_tag="BMJ25_RS25890"
                     /old_locus_tag="BMJ25_25665"
     CDS             7705..9018
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011976337.1"
                     /locus_tag="BMJ25_RS25890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BMJ25_25665"
                     /product="glucoamylase family protein"
                     /protein_id="WP_011976337.1"
                     /transl_table=11
                     /translation="MSLHSRTSDIACIDGLQRAAFEYFLRYSDMRSGLVADTSRAGSPS
                     SIAATGFGLAAYPVAVERGWIARSDAAWRVLTTLRFFAASRQGSDASATGYRGFYYHFL
                     DMRTGDRVWRSELSTIDTSLLLAGALTAAAYFSGRSEEESELRRLAALLYERADWYWAL
                     NGGDTVTMGWRPPGRFLKHRWRGHSEALLLYVLALGSPTRPIMPPNYEAYTAAHEWLEL
                     DGAAHLHAAALFVHLFPQAWIDFRSIRDKGMRDRATDYFDNTRRAIRLQRAHAEENPHG
                     FAGYCRDLWGFSACHAPKGWFRLRDGRRQKLLGYEARGAPFGADDGTLVPWAPLAGLPF
                     EPDACLGSLFHLMSRYPALVKEERLPGGFNPSLPGDGPEGWMDDRLVGLDQGLVVMMIE
                     NWRSGLVWELTRGIPAFRQGLSRAGFAGGWLSSTLLQV"
     gene            9068..9547
                     /locus_tag="BMJ25_RS25895"
                     /old_locus_tag="BMJ25_25670"
     CDS             9068..9547
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011976336.1"
                     /locus_tag="BMJ25_RS25895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BMJ25_25670"
                     /product="anthrone oxygenase family protein"
                     /protein_id="WP_011976336.1"
                     /transl_table=11
                     /translation="MVTLVPVLAFAAVIGSGLMAGLFFVFSVCIMQALSRLPPEQGVAV
                     MNAINVVIQNPLFLIAFMGTALLGLVLLVMAVIWGGQGSYFLAAGGLVYLIGTLAVTIA
                     VNVPLNEALAAAPAGQGATELWQQRYLTEWVRWNHVRSFASTAALALFVLGFART"
     gene            complement(9561..10352)
                     /locus_tag="BMJ25_RS25900"
                     /old_locus_tag="BMJ25_25675"
     CDS             complement(9561..10352)
                     /NRPS_PKS="Domain: ECH (7-254). E-value: 4.8e-65. Score:
                     211.2. Matches aSDomain:
                     nrpspksdomains_BMJ25_RS25900_ECH.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1023: enoyl-CoA hydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018095567.1"
                     /locus_tag="BMJ25_RS25900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BMJ25_25675"
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="WP_011976335.1"
                     /transl_table=11
                     /translation="MSDTVLLKISGGTALLTLNRPERLNALNYELIDRLLTLLDRLEVD
                     AKVHAVILTGAGDRAFSAGGDIHEFSHSVAEGVNAAIRDFVRRGQQLTSRLEAFPKPVI
                     AAVNGIAYGGGCEITEAVHLAIASDRSRFAKPEIKLGMPPTFGGTQRLPRLAGRKRALE
                     LLLTGDAFSAERALEMGLVNAVVPHADLIETARALAARIISHSPVATAAIISAVTRGLN
                     MAIGEGLLCESEQFARMVPTHDLNEGLAAWKERREPRYAGR"
     aSDomain        complement(9591..10331)
                     /aSDomain="ECH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BMJ25_RS25900_ECH.1"
                     /evalue="4.80E-65"
                     /label="BMJ25_RS25900_ECH.1"
                     /locus_tag="BMJ25_RS25900"
                     /protein_end="254"
                     /protein_start="7"
                     /score="211.2"
                     /tool="antismash"
                     /translation="KISGGTALLTLNRPERLNALNYELIDRLLTLLDRLEVDAKVHAVI
                     LTGAGDRAFSAGGDIHEFSHSVAEGVNAAIRDFVRRGQQLTSRLEAFPKPVIAAVNGIA
                     YGGGCEITEAVHLAIASDRSRFAKPEIKLGMPPTFGGTQRLPRLAGRKRALELLLTGDA
                     FSAERALEMGLVNAVVPHADLIETARALAARIISHSPVATAAIISAVTRGLNMAIGEGL
                     LCESEQFARMVPTHDLNEGLAAWKE"
     misc_feature    complement(10326..10340)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 18.34"
                     /tool="antismash"
     misc_feature    10335..10355
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 22.49"
                     /tool="antismash"
     gene            10436..11044
                     /locus_tag="BMJ25_RS25905"
                     /old_locus_tag="BMJ25_25680"
     CDS             10436..11044
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011976334.1"
                     /locus_tag="BMJ25_RS25905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BMJ25_25680"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_011976334.1"
                     /transl_table=11
                     /translation="MAATQNTRERIVSAAARLFYAEGIRAVSVDAVAEAAGLTKRTLYY
                     HFKSKDELIAAYLEERDQPNLKLFQKWFSETDGDAASKIKAIFLNLARAARHPKWKGCG
                     FLRTSAELANMPGHPAIRIAAAHKRKFEDWLRQSFETEGIAEAARLARQMLLLLDGSFA
                     VVLLHRDPSYMETAGEAAATLVKLALSEPSVQQPAGRGK"
     misc_feature    complement(11060..11076)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.82"
                     /tool="antismash"
ORIGIN
        1 tgcagtcatc tcccaccact tcatcaaact caataatcga catagggtta ttcagaacct
       61 cgacgccatc agcccactgc cgaaaaattg cccccttagc tccttcgcct ccaagcgtcc
      121 tagacacaag gcgcgtgtcg aacatgttcg tgtggccata atagccgccc tgaaattccg
      181 cctttgaagt cagggatttg atcggctgat agagccacac caccggcttc gggagtgcca
      241 agttcgtgac tgggtgactt acgtcgaacg cgtatccatg acccttcaac gacatcattc
      301 catgattatc gccaccgtga aggagagaca tctctgctgg atgtggaagg ccgcagccct
      361 ttgaacaaaa gaaatctgaa gagatgttaa acagtagcag accgtttatc ctcagtccga
      421 aacaactggg catagatgct gagatcatgc tgtcgtttcc gaaaaggttg atgcctggca
      481 actggctatc gaacacataa accgcaagca tccgatcctt acggcccatt cgagacgcga
      541 tatgaaaatt cggatcaact gcaattgcgt ttttctcgat cattcctctg agcagccaga
      601 ccccgatgcg gactttatcc agccaatcga gcaactcgtc atattggctc acagttagag
      661 cctcgcgccg ctccaaggcc tcgatggtcg gtttaatccg agcctctagc ttcgagaatg
      721 tgttgttaca gtcatcacag gccggagcga caaaagcttg ccaagcataa gatatcggag
      781 cttttccctt tgtgtaatcg aggcccaaag ttacgactcg cttcggatca cccgtcatct
      841 caagcagcca gtatgggacc acgtgctccc tggtcttgga gttgggcgtc ttcccgcaaa
      901 aaacacagaa ctttcgcgat ttcgtctctg acatggtgga tgctgtccct agatggccgg
      961 tacccgataa ggtgagaggc aacagctaaa actcgtaaac tgctgatgat cttacatggc
     1021 cagatgcacc attacacact aaagttgtgg aattcgactg ttcgtatttc tgttcaaacg
     1081 tatgtggcga cggacaccat tttgctcagg ggtatgaggc gacgtgctca aatgaacgtg
     1141 gcgacggcgc tggaaaaggc agtgttttca acattgccaa gttcaaacgt atgtggcgaa
     1201 cagcaccctc attgaacgtc gtttttcctt cattttcctg ggtctgtctc gccggaattc
     1261 tgcttaggcg aaagcgaaac ctcccgggcc acatatgtct tgcagcgctc gaaacctgaa
     1321 tcgatgatgc gctcgggtcc gcacggcttc gtatagtgct gctgcgccat tcctgcgttg
     1381 atcgcggcat ggggcgctgc gcggaaatcg ccgagagcgt tctggtgtcg ctatcctgag
     1441 ccggaacgga gaccgctgat gccccgctgg aagaagccga ccaaagaaga agtcctggaa
     1501 aatcgacgcg atctggcgaa ccgtgcgcgt agcggtgagc tgcgcttgcc ccatgccgtt
     1561 gctgaaattc gcaagggctt cggcatgacg caagaggagt ttgcgaagat gctggcgctc
     1621 accaggaggc aggttgccga aatcgaagcc ggcacagcga acccgacatt ggagacattg
     1681 gaaaaaatgg ccgtctattc ggctttgggg tgggtttcgt gccggggacg ataaagaaaa
     1741 ctgagagcga gcgtgatccg gcgccgaaag gctctcacga acgcacatag ccgatcgact
     1801 ggcgtgctgc atcgcattct tcgcacgcca cttctgccgg atgcaggaag acgagcgggt
     1861 cgtgggccaa ttgttccatt gcgattttca cgatgtcgcg tgagcggacc aggcgggcat
     1921 gggcgttgcc gatctgcccc tccttctgga ggccgtttcg cctcaaccag acgtcggcca
     1981 ggctgaagtt ctggcaacag tgatcattct ccagataggc aatgcggacc cggctgccat
     2041 tttcgacgat cgtgcagtgt ctcggcactc catagggaac cttggacatc agctcggcga
     2101 gatgcaaggt ggtgttcgaa tcatgtccga cgccgaggag tatcacctgt ccgtcgagtt
     2161 catggatacg ccctatcgga ctggggggac aatgcggggg caggggcagg gggtccgatg
     2221 tgatgcgtgc ggcaagcggt cccgccgctg caaaggcgaa cggatggtcg ctgcgcacca
     2281 caccctcaag acgccagaaa gtatcggcga gtattcccag atccgatgtt gctgaggtcg
     2341 tcgccggctc gaatggctcg tcgtcaaggc ccgaccagga gggcatgacg agcgtgcctt
     2401 ccgggccgat ggcggtgcgc agagcggcga tcatgccggc cggaccgcct tcgaccggcc
     2461 ggacgctgcg aaacgaagag tgaacgacaa gaacgcctcc aggcttcacc ccgagggtga
     2521 ggagttgatt gacgagttcc gaaatgcgcc attcacgttg cgtcatggtt gtaccggatg
     2581 agtccacttt aattggtgtt ctgacagcgc ttgctcacgg tatcggcttc gaacgcgttg
     2641 aaaatagcgt tgaggcgggc gtggaacaat aatggggagc tccgtcggcg ctttcaggtt
     2701 ccccttggga gctataagcg gcatcggttg ccatgttccg ccttttcaag catgttcatg
     2761 catggctgca tctgggctga aaaccgcgca cactggctat ccgctctgcg gaccgaggtg
     2821 tgattgccct aaggggtgga agagggctgg gtgaacgaga tttcgatgca ttcgccggcg
     2881 gcggccggtg gtgacctaaa atcatattga tctaaagccg tttggggttt tcgtggcggt
     2941 gccgcatttt catctgcaga cattcgggga attgcggctg gtcgattccg gcggcgtggt
     3001 cgtggcgtgc ccggagcggg gcctgcttat tctggcctac ctcagcatat ccgggcagag
     3061 aacggtctcc cgcgaaaagc tctcgatgct gatctggccc gagcgggaga agagcgtcgc
     3121 tttcaagaac ctgcgctcta ccctgtggcg catgcgccac cttgccgcga actcggggcc
     3181 gctcacgtcg gcgacggaaa tagatgtgac gctgggggag attgcctgcg acgccgccgg
     3241 tttcgaggcg ctcgggagct cggaggaagc gtttcgcgcc gggctctcgc tctggcgcga
     3301 tacgttcctg gcaggcgccg atgtgcctcc cgggcgttat gcggactggg ttggagagca
     3361 gcggagcgtg tttacgcaaa agctgcgcgc ggcactttta gccggcggag agcgatttgc
     3421 cgacaggtcg atgctgcgga cggcgtgcat gcatctcctc gaactcgatc cggcggatac
     3481 tgccgtgcgc gcaatcctgg agcggttgac cggcgtgggt ctcgtcatcg ggaaaggctc
     3541 gccggacggg gcctggctgc ggcagggggc ctttgcaggg gagtcgcccc gggcgccggc
     3601 aagggatgcg ataaccccgc cggttgcgcc cgcaccgcgc gttgcgctgc ttccgcccgc
     3661 ggccttcggc agtgatccga tgcttcacgc ggtcagcatg gccgtggtcg aagacatcac
     3721 gatcggatta tgcgcgctgc gctcgatgtc ggttgttgcc ccttacactg ccgagcgtat
     3781 tcgggatgcg gcggacaagg ctgccttcct ggaaaaacat gccgtcactt atgcgctcga
     3841 ctgtcgcatg tcggaccagg ggctgtttac tcaactcatc ttcctgccgt ccgacgcgat
     3901 cgtctgggcc gagcgctttt cgatctctcc ggtcgggctc ctgcaacagc gtcaggaaat
     3961 tgcctttcac gtggccagcg cggtagcgga acaggtggag accgggcgta tcgggcacct
     4021 cgattacgtg accaatccgg acgcctatta cgcctatctc gccggcctgc gaaacctttc
     4081 gaacgtcggc cttcccgaaa tccgcagggc acgccgcgat ttcaagacgg cgctcaggca
     4141 caagccggat tttgccccgg cgctaagcgg catcgcacgc acctatgcga tcgagtgggt
     4201 cctgacggct cggggcgacc aggagctttt gacggtagcc gaacgtcacg ccaaggaagc
     4261 gatcgagagc gatgaagagc ttcccggagc acatcgcgaa ttcggcgtcg tcagacttta
     4321 tcagggcgac ctcgatggaa gtcttgcggc gctgaaccgc gccgaaaatc tgagcccgca
     4381 ttatgccgac gtgctgtaca gccatgcgga cacgctcgtc catgcctccc gcccgcgtga
     4441 ggctctcgac aagctcggca aggcgctttc gcttaatccc ctggcgccgg acatgtattt
     4501 ctggagcgct gccggagcca gctactttct cgaagagtat gaggatgcga tcggctacgt
     4561 tcagaaaatg aaggacaagt cgccgggcga ccggctgctt gcagcaagct gggcgatgct
     4621 cggcgatcag aagaaagcgc ggtcatacaa ggtgaaggcc ctgagggcca atccgacatt
     4681 tgatgtcgac aagtggctcg ccgtcgttcc gatgaaggaa caatggcaga aggacctcta
     4741 tcgcgagggg ctgaaaagag cggggttttg atgatgaggg gaagcgatat ggcagaagtc
     4801 gtagtgattg gtattgccgg cgagcacggt ttgtggctgg cggatctgaa aaatggaacg
     4861 gtcaggccgc tcgatcaggt gtcgggcgat ctcgccacgg cgaacaagct ccgcctgcag
     4921 ggcgggacga tcgtcaagaa tgttgatctc gcagtagcgg tgtcgtcggc cgacaaggtg
     4981 ttttcggggc atttcgacgg gtgatcggct tcgctgtcgg cagtcctgca gacacgcgca
     5041 atgcttattc cgatcgcgtt ctgagcccgg aagagacgtt cgaccgtgtc cggccactgc
     5101 tttcccggtt cggcgtgacc cgcatcgcac ggcacacggg cctcgacagg atcgggctcc
     5161 cggtctggtg cgcctacacg ccgaacgcac gatcgatcgc ggttgcgcag ggcaagggat
     5221 tgacggatcc ggaggcgaag gtttccgcgg tcatggaggc gctggagcgc gccgtcgcct
     5281 gcgagcccgc cgtcggaaca atggtcgcca cggcccggac cttgcgtgca tccggacgga
     5341 ccgtggagct gcttccctgc ctgattgcgg ccgggcagca ggatataggg ccggacgaag
     5401 aactcgcatg ggccctcggc tccgacctga tctccggccg cgagatactc gtgccgctcg
     5461 cagcggcgct gctcgaccgt accctgcatt cgcgtttctg gatgtcgtcg gatggacttg
     5521 cgtcgggaaa cgtcgtggaa gaagcgatat tgcacggact gctggagcgc atcgagcgag
     5581 acgcgcacgt cctctggggc atctcggggc gggagcaccg ttacgggtgc tgtgtcgctc
     5641 ccgagagcct gggcgatgca gcgttgaacg gtctcgttga gcgcattggg gccgccggac
     5701 tcgatctccg gcttttcgaa atcaccagcg acataggcat tccctgtttc ctggcgctgc
     5761 tcggcccacc cgatatcgcg accaggggcg gtggccgctt cgtcgaagtg acgagcggct
     5821 gcggcgcaca tgtttttccc gcccgggcgg cgatcagggc ggtgacagaa gcggcgcagt
     5881 cccggctcac cttcatcagt ggtgcccggg acgatgtctt tcccgagacc ttcaaccggc
     5941 ccttgccgga actgacgcgg caggccttcc tcgccgagcc atccagacgg gccatcgcca
     6001 gcccgcaggt cggcggaagc cttgaagcgc tgctgcgcca caccgttgat cacctgacga
     6061 gcgccggcgt gcaatctgca attgcgcttc gtctgtcggc gaacgcgctt ccgttctcgg
     6121 tcgtcaagat cttcgttccc aaactcgaga atccggatgg tgatcgagcg cgccgttttg
     6181 gtccgcgtgc actatccaaa gcgctcgcat cgtgacggga gcgggcaatg gggctccggg
     6241 cgaagatccg ctaaatactc cacctgagcg tgtgcaacgc tcgtgaaacg cgcggaaatg
     6301 ccgaactcgt ttatagtcgc cctcatcaca ggcatggatt gggggttcat atgactaatc
     6361 attcttcgtc cttgacggat accgaggtgg aacttgcgag cattgcccga atgttgagcc
     6421 acattcgcga gagcgctcgt gccttggagg cacaggccgt cgagtattgc atcgatatgg
     6481 cactgcaggc ggtcgccaac gaattgcggg atcgcggcga gctcgtcatt gccgctgcgg
     6541 gcttcgacaa tagaacgacc ctgctgcaat agcgcgctgc ggggaaaccc gcctgcgctg
     6601 ctaccgcacc cgaccgcact tctttcgtcc tcaccattcc tgtggcatgc aggaaggtgg
     6661 ggatatgaag agcaagcgtc agcgatccag ttccggatag accctgtcga gaaatcccgg
     6721 ccggagcttc tcgcctatca tgcagtccgc cgcattttcg ccggccgcca ggcggagcgc
     6781 cggatcgggc tcgcgcccgc ttacctcgag gatcagcttc tgacggattg cgaccgcgaa
     6841 atcctcgggt tcatgcacgg gcaacacgaa ggagccgggg ccgccaatga cgcattcggc
     6901 gtaataccgg tcgagcggcc cggtcgagac cgaagggcgg atgaggattg caagaccgtt
     6961 gattataatg ccccgcgata gggcgtcgtt gcgtgcgggt gtaaccggcg gcccggtatt
     7021 gttcggcccg tcgccggaga tgtcgatgac ccttcgcgta cccgaatatg cgttcgaatc
     7081 gatgaggccc gaaccaaaca ggatcgcgtt cgagatcgag gtgccgcgcc gtgttccgat
     7141 tggccgagcc tccagcttgc cggcgaaggc ttcggcgtcg gcagaacttt cgatcacttg
     7201 ccagctcacg actgaggcct cgtttacgag ccctgcccat tcgaaatagc ctatggcgat
     7261 ccgcccgagg aggcctccct ggaccgcatt gatgaaatcc ggatgccgca gcgcctgcag
     7321 atatccggag cgctgcacgc gcgcctcctc catgtccatc gagccggaca tgtcgacggc
     7381 cagcaggagt tctacgtcga cgtcagcgga aatggctgcc gactgtatcg gagctccgct
     7441 cagtgccagg atcaatgcca tcgtgcctaa catcgcatca gccaatcacc gtggatcacg
     7501 cgtcgggcgt tctgcccctg cacccgggta ccgcgaggcg ccttagagcg atgtcgccgt
     7561 ccgggtcgat gtcattccat gaatgtatca caggttttga gatcggcgac tctcggagcg
     7621 atgcagacct acaatattcg gtgatcgcgg ttggtctgcg tggcctgccg caatgtgacc
     7681 aacctcgtcg accagtaggc tggcatgtct ctccacagcc gcacatcgga tatcgcctgc
     7741 atcgacgggc tgcagcgcgc cgccttcgaa tactttcttc ggtattcgga catgcgaagc
     7801 ggcctcgtcg ccgacacgtc tcgcgcgggt tctccgtcga gcatcgccgc cacgggtttc
     7861 ggacttgcgg cctatccggt cgcggtcgaa cgcggctgga tcgcccggtc ggacgctgcc
     7921 tggcgcgtgc tgaccaccct ccggtttttc gcggcaagcc gtcaggggag cgatgcgagc
     7981 gcgaccggtt atcgcggatt ctactaccat ttcctcgaca tgcggacggg ggatcgcgtc
     8041 tggcgcagtg agctctccac gatcgacacc agcctgctcc tcgccggtgc cttgacggcg
     8101 gcagcttatt tctctggccg cagcgaagag gagagtgagc tcaggcggct tgcggcgctc
     8161 ctctacgaga gggcggattg gtactgggcg ctgaatggcg gcgacacggt cacgatgggg
     8221 tggcgcccgc cggggcgatt tttgaaacac cgctggcggg gtcatagcga ggcgctgctc
     8281 ctctacgtcc tggcactcgg ctcgcccacg cgcccgatca tgccgccgaa ctacgaggcc
     8341 tacacggcag cgcatgagtg gctggaactc gatggggcgg cgcatctcca tgccgccgcg
     8401 ctcttcgtcc acctttttcc gcaggcatgg atcgattttc gcagcatccg ggataagggg
     8461 atgcgcgaca gggccaccga ctatttcgat aacacccgcc gcgccatccg gctgcagcgc
     8521 gcccatgccg aggaaaaccc tcatggattc gccggctatt gccgcgacct ctggggtttc
     8581 agcgcctgtc atgcgccgaa aggctggttt cggcttcgcg acggccggcg gcagaagttg
     8641 ctgggctacg aggcacgcgg cgcaccgttt ggtgccgacg acggaacact cgttccctgg
     8701 gcgccgctgg ccgggctgcc attcgaaccg gacgcgtgtc tcggcagtct ttttcatctg
     8761 atgtcgcgtt accccgccct ggtgaaggag gaacgccttc ccggcggttt caatcccagc
     8821 ctgccggggg acggtcccga gggatggatg gacgatagac tcgtcggcct tgaccaaggg
     8881 ctcgtcgtca tgatgatcga gaattggagg agcggcctgg tttgggaact gacgcgcggc
     8941 attccggcct ttcgtcaggg gttgagcagg gctggatttg cgggaggctg gctttcgtcg
     9001 acgctgcttc aagtctgaaa cgagagaagc gacccgaagc ggctcaattc cctcctggag
     9061 tgttgcgatg gttacccttg ttcctgtgct cgcatttgcc gccgtgatcg gatccggcct
     9121 gatggccggg ctgttcttcg tcttttcggt ctgcattatg caggcgcttt cgcgactccc
     9181 gcccgagcaa ggcgtcgccg tgatgaacgc cattaacgtg gtgatccaga acccgctgtt
     9241 tctcatcgcc ttcatgggaa cggcgctgct tggcctggtg ctcctggtca tggccgtcat
     9301 ctggggaggg caggggagct atttcctggc tgctggcgga ctcgtctatc tcatcggcac
     9361 gctcgcggtg acgatagccg tcaacgtgcc gctgaacgaa gcgcttgccg ccgcccctgc
     9421 cggacaagga gccaccgaac tctggcagca gcgctatctg accgaatggg tccgctggaa
     9481 ccacgtgcgc agcttcgctt cgaccgctgc tctggcgctc ttcgtcctcg gcttcgcccg
     9541 gacctgatga tcgggcaatc tcagcggccg gcatagcgcg gctcgcgccg ctctttccag
     9601 gcggcgaggc cttcgttgag gtcgtgggtc ggaaccattc gtgcgaattg ctcgctctcg
     9661 caaagcagac cttcgccgat cgccatgttg aggccgcgcg tcacggccga gatgatagcg
     9721 gcggtcgcaa caggagagtg gctgataatg cgcgccgcga gcgctcgggc ggtctcgata
     9781 agatccgcgt gcggaaccac cgcatttacc agccccatct ccagcgcgcg ctcagccgaa
     9841 aacgcgtcgc cggtgagaag cagctccagc gcgcgcttgc gtccggcaag gcgcggcagt
     9901 ctctgcgtgc cgccgaaagt gggcggcatg ccaagcttga tctccggctt ggcaaatcgc
     9961 gaccggtcgc tcgcgatcgc gagatgcacc gcctccgtga tttcgcagcc gccgccatag
    10021 gcgatgccgt tgacggcggc gatcacaggc ttcggaaagg cctcgagacg gctggtaagc
    10081 tgctgccctc ttcgcacgaa atcgcggatc gcggcgttca cgccttcggc cacgctgtgg
    10141 gagaattcgt gaatgtcgcc gcctgccgaa aaggctcgat cacccgctcc ggtgaggatg
    10201 acggcatgca ccttggcgtc cacctcgagg cgatcgagga gggtcaaaag ccggtcgatc
    10261 agctcgtaat tgagcgcatt cagcctttcg ggccggttga gcgtcaggag ggcggttccg
    10321 cccgaaattt tcagaagcac agtatcggac atgtttgtct cccgtcgggt gttgacgaac
    10381 ggaggctaag tgggagacaa aggaatgtat attcactagt cggtatacct ggattatggc
    10441 ggcgacacaa aacacacgcg aacgtatcgt ttccgccgct gccaggctgt tctatgcgga
    10501 aggaattcgc gccgtcagcg tggacgccgt tgccgaggcg gccggcttga cgaagcgcac
    10561 tctctactat catttcaaga gcaaggacga gttgattgcc gcctatcttg aggagcgtga
    10621 ccagcccaac ctcaagctct tccagaagtg gttcagcgaa accgacggtg atgcggcgtc
    10681 aaagatcaaa gccatttttc tcaatctcgc ccgggctgcc cggcacccga aatggaaggg
    10741 ctgcggtttc ctccgcactt cggcggaact cgccaatatg cccggccacc cggcgatccg
    10801 catagcggca gcgcacaaaa ggaagttcga ggactggctg cgtcagagct tcgagacgga
    10861 aggaattgca gaggcggcca ggctcgcgcg gcagatgctg ctgctcctcg acggcagctt
    10921 cgcggtcgtt ttgctccacc gtgatccgag ctatatggag acggcgggcg aggccgcggc
    10981 tacgctcgta aagctggcgc tttcggaacc gagtgtgcag caaccagccg ggaggggcaa
    11041 atgaaagtcc gacgcatcat gcctaatttt caggtatccg atctgctcga ggcagatcgc
    11101 ttctacaggg ccctgctcgg cctggagatc gtgatgaatc acggctggat cgtcacctat
    11161 gcggcggatg gcagttccgc catgccccag gtgagcttcg ccaccgaggg cggat
//
