LOCUS       NZ_NBUK01000015        20843 bp    DNA     linear   CON 19-MAR-2024
DEFINITION  Sinorhizobium medicae strain Str8 scaffold9.1, whole genome shotgun
            sequence.
ACCESSION   NZ_NBUK01000015
VERSION     NZ_NBUK01000015.1
KEYWORDS    WGS; RefSeq.
SOURCE      Sinorhizobium medicae (Ensifer medicae)
  ORGANISM  Sinorhizobium medicae
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
REFERENCE   1  (bases 1 to 487157)
  AUTHORS   Porter,S.S., Faber-Hammond,J.J. and Friesen,M.L.
  TITLE     Co-invading symbiotic mutualists of Medicago polymorpha retain high
            ancestral diversity and contain diverse accessory genomes
  JOURNAL   FEMS Microbiol Ecol 94 (1) (2018)
   PUBMED   29228264
REFERENCE   2  (bases 1 to 487157)
  AUTHORS   Porter,S., Friesen,M.L. and Faber-Hammond,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2017) Biology, Washington State University
            Vancouver, 14204 NE Salmon Creek Ave., Vancouver, WA 98686, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: AllPaths v. May-18-2012 Linux x64 A5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: GCA_000017145.1
            Genome Coverage                   :: 33.6x
            Sequencing Technology             :: Illumina Genome Analyzer IIx
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002865145.1-RS_2024_03_19
            Annotation Date                   :: 03/19/2024 04:22:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,364
            CDSs (total)                      :: 6,308
            Genes (coding)                    :: 5,904
            CDSs (with protein)               :: 5,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 404
            CDSs (without protein)            :: 404
            Pseudo Genes (ambiguous residues) :: 0 of 404
            Pseudo Genes (frameshifted)       :: 278 of 404
            Pseudo Genes (incomplete)         :: 206 of 404
            Pseudo Genes (internal stop)      :: 43 of 404
            Pseudo Genes (multiple problems)  :: 106 of 404
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:52:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 66345
            Orig. end                         :: 87188
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NBUK01000015.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..20843
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene"
                     /region_number="1"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..20843
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20843
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:10843](-)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10843)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     misc_feature    complement(948..961)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: medium, score: 20.74"
                     /tool="antismash"
     gene            986..1348
                     /gene="gcvH"
                     /locus_tag="BMJ25_RS03970"
                     /old_locus_tag="BMJ25_03960"
     CDS             986..1348
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_process="GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /gene="gcvH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529528.1"
                     /locus_tag="BMJ25_RS03970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BMJ25_03960"
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="WP_011975352.1"
                     /transl_table=11
                     /translation="MLKFTEEHEWLKIDGDVATVGITEHAAGQLGDLVFVELPEAGSTF
                     SKGDTAATVESVKAASDVYCPLDGEIVEANQAIVDDPSLVNSDPQGAAWFFKLKLADPD
                     AAKALLDEAAYKELVA"
     gene            1348..4212
                     /gene="gcvP"
                     /locus_tag="BMJ25_RS03975"
                     /old_locus_tag="BMJ25_03965"
     CDS             1348..4212
                     /EC_number="1.4.4.2"
                     /GO_function="GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA]"
                     /GO_process="GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /NRPS_PKS="Domain: Beta_elim_lyase (535-679). E-value:
                     3.2e-09. Score: 28.4. Matches aSDomain:
                     nrpspksdomains_BMJ25_RS03975_Beta_elim_lyase.1"
                     /codon_start=1
                     /gene="gcvP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017960600.1"
                     /locus_tag="BMJ25_RS03975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BMJ25_03965"
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="WP_024311824.1"
                     /transl_table=11
                     /translation="MSMPKDFTFTDYKPYDFANRRHIGPSPAEMDEMLKVVGYPSLDAL
                     IDDTVPPSIRQQTPLAWGAPMTEREALDKLRETANRNRKVVSLIGQGYYGTITPPVIQR
                     NILENPAWYTAYTPYQPEISQGRLEALLNFQTMVCDLTGLDVANASLLDEATAAAESMA
                     IAERVAKSKAKAFFVDENCHPQTIALLKTRAEPLGWQIVVGNPFEDLDAASVFGAIFQY
                     PGTYGHVRDFSGLIARLHELGAIAAVAADPLALALLKSPGEMGADIAVGSTQRFGVPVG
                     YGGPHAAYMAVRDAYKRSMPGRLVGVSVDARGNRAYRLSLQTREQHIRREKATSNICTA
                     QVLLAVMASMYAVFHGPEGIKAIAQSVHQKTVRLALGLEKLGYTVEPDVFFDTITVEVG
                     KLQGIILKAAVAEDVNLRKIGTTKIGISLDERSRPITLEAVWRAFGGDFSVDQFEPDYR
                     LPKELLRTSDYLTHPIFHMNRAESEMTRYMRRLADRDLALDRAMIPLGSCTMKLNATAE
                     MLPITWPEFSEIHPFVPADQAMGYHHLIEDLSQKLCAITGYDAISMQPNSGAQGEYAGL
                     LAIRAYHIANGNEHRDVCLIPTSAHGTNPASAQMAGMKVVVVKVSDAGEIAMDDFRAKA
                     EQYAETLSCCMITYPSTHGVFEENVREVCEIVHKHGGQVYLDGANMNAMVGLSRPGDIG
                     SDVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFLPGHPESGEHEGAVSAAPFGSASI
                     LPISWSYCLMMGGEGLTQATKVAILNANYVAARLKGAFDVLYKSAKGRVAHECIIDTRP
                     LAESAGVTVDDVAKRLVDCGFHAPTMSWPVAGTLMIEPTESETKAELDRFCDALLAIRE
                     EARAIAEGRMDKINNPLKNAPHTVEDLVGDWDRPYSREQACFPPGAFRVDKYWSPVNRV
                     DNVYGDRNLVCTCPPIKSYAEAAE"
     aSDomain        2953..3384
                     /aSDomain="Beta_elim_lyase"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BMJ25_RS03975_Beta_elim_lyase.1"
                     /evalue="3.20E-09"
                     /label="BMJ25_RS03975_Beta_elim_lyase.1"
                     /locus_tag="BMJ25_RS03975"
                     /protein_end="679"
                     /protein_start="535"
                     /score="28.4"
                     /tool="antismash"
                     /translation="DQAMGYHHLIEDLSQKLCAITGYDAISMQPNSGAQGEYAGLLAIR
                     AYHIANGNEHRDVCLIPTSAHGTNPASAQMAGMKVVVVKVSDAGEIAMDDFRAKAEQYA
                     ETLSCCMITYPSTHGVFEENVREVCEIVHKHGGQVYLDGA"
     gene            complement(4602..6077)
                     /gene="tig"
                     /locus_tag="BMJ25_RS03980"
                     /old_locus_tag="BMJ25_03970"
     CDS             complement(4602..6077)
                     /EC_number="5.2.1.8"
                     /GO_component="GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA]"
                     /GO_function="GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /GO_function="GO:0051082 - unfolded protein binding
                     [Evidence IEA]"
                     /GO_process="GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /gene="tig"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012651321.1"
                     /locus_tag="BMJ25_RS03980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BMJ25_03970"
                     /product="trigger factor"
                     /protein_id="WP_018208599.1"
                     /transl_table=11
                     /translation="MQVIETLAQGLKRELKVVIPADEMEARMNERLVEVKDRVRINGFR
                     PGKVPVAHLKKVYGKSIMADLVNEIVREKPTEILTSRGEKSATQPEIGMTEDEAEADKI
                     LKAEADFEFTVAYEIIPPIELKDASGIKVTREVVDVGEDEVNEQIERIAESARTYESKK
                     GKAANGDRVTIDYLGKVDGEAFDGGKDEDAELVLGSNRFIPGFEEQLVGAKAGDEKTIT
                     VTFPADYPAANLAGKEATFDVTVKDVAAAAPIEINDELATKLGLESVDKLKEIVRGQIE
                     SQFGSITRQKVKRQLLDQLDELYQFDTPERLVDAEFENIWRQINTDLQQAGKTFADEDT
                     TEEEARAEYRKLAERRVRLGLVLSEIGEKAGVQVSDDEMQNSLFQQLRQFPGQEKEIIE
                     YFRNTPGAAASLRAPLFEEKVVDHLLTEVSVTDKKVSKEELTAEDDADEKPAKKTASKK
                     KAAAKADAAEGEEAAAPKRKAPAKKKASDESAE"
     tRNA            complement(6253..6337)
                     /anticodon=(pos:complement(72646..72648),aa:Leu,seq:tag)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="BMJ25_RS03985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="BMJ25_03975"
                     /product="tRNA-Leu"
     gene            complement(6253..6337)
                     /locus_tag="BMJ25_RS03985"
                     /old_locus_tag="BMJ25_03975"
     misc_feature    complement(6821..6837)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: medium, score: 21.52"
                     /tool="antismash"
     misc_feature    7240..7256
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 19.7"
                     /tool="antismash"
     gene            7338..7481
                     /locus_tag="BMJ25_RS03995"
                     /old_locus_tag="BMJ25_03985"
     CDS             7338..7481
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529522.1"
                     /locus_tag="BMJ25_RS03995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BMJ25_03985"
                     /product="DUF1127 domain-containing protein"
                     /protein_id="WP_003529522.1"
                     /transl_table=11
                     /translation="MNLARSFNNWRKYRQTCNELGRMSDRELTDLGIGRADIPYVARQA
                     IK"
     gene            7777..7920
                     /locus_tag="BMJ25_RS04000"
                     /old_locus_tag="BMJ25_03990"
     CDS             7777..7920
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529520.1"
                     /locus_tag="BMJ25_RS04000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BMJ25_03990"
                     /product="DUF1127 domain-containing protein"
                     /protein_id="WP_003529520.1"
                     /transl_table=11
                     /translation="MNPIRIAKSWINYRRTVAELGSLSNHALNDIGITRYDIRNIAARS
                     FR"
     gene            8012..9454
                     /gene="trmFO"
                     /locus_tag="BMJ25_RS04005"
                     /old_locus_tag="BMJ25_03995"
     CDS             8012..9454
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /GO_process="GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /gene="trmFO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018011009.1"
                     /locus_tag="BMJ25_RS04005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BMJ25_03995"
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="WP_018011009.1"
                     /transl_table=11
                     /translation="MNKLTSSYSPVHVIGGGLAGSEAAWQIAEAGVPVILHEMRGVRGT
                     DAHKTDSLAELVCSNSFRSDDATSNAVGVLHAEMRLAGSIIMRCADLNQVPAGGALAVD
                     REGFAEAVSAAIAAHPLITVLREEIRGLPPKEWDLAIIATGPLTAPDLADAIRAETGAD
                     ALAFFDAIAPIVHADTIDMDICWHQSRYDKVGPGGTGKDYINCPLTQEEYNAFVDALIA
                     GDKTGFKEWEGTPYFDGCLPIEVMAERGRETLRHGPMKPMGLTNAHNPSVKPYAVVQLR
                     QDNALGTLYNMVGFQTKLKYGAQGEVFRMIPGLEKAEFARLGGLHRNTYINSPTLLDQS
                     LTLKSRAGLRFAGQITGCEGYVESASIGLLAGRFAAAERKGASPAIPPVTTAFGALLNH
                     ITGGHIVSDDEPGKRSFQPMNVNFGLFPPLDPGALTRPEGAKRFRGKEKASAKKQAMAS
                     RALSDCATWLDKNLLIGGAATR"
     gene            complement(9464..9919)
                     /locus_tag="BMJ25_RS04010"
                     /old_locus_tag="BMJ25_04000"
     CDS             complement(9464..9919)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015241188.1"
                     /locus_tag="BMJ25_RS04010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BMJ25_04000"
                     /product="hypothetical protein"
                     /protein_id="WP_011975347.1"
                     /transl_table=11
                     /translation="MLWLLFPAAIAAVCVFYIRMNARAERDADNPDAGSAIVEFGRAFP
                     DEAIRALHSSVDGTAIFLRLHDGKAGFIQSHGTHYVCHVVQPDKVRVNTSDSGRGLIVH
                     FPDFAYLDNAFEFRTAAEAAEVSLWLLGSFRTKLEKEASGNAAPGPD"
     gene            complement(10001..10843)
                     /locus_tag="BMJ25_RS04015"
                     /old_locus_tag="BMJ25_04005"
     CDS             complement(10001..10843)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: PT_phytoene_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: phytoene_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529514.1"
                     /locus_tag="BMJ25_RS04015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BMJ25_04005"
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="WP_011975346.1"
                     /sec_met_domain="PT_phytoene_like (E-value: 1.5e-15,
                     bitscore: 52.0, seeds: 61, tool: rule-based-clusters)"
                     /sec_met_domain="phytoene_synt (E-value: 3e-22, bitscore:
                     73.9, seeds: 8, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQQSDDQILATLRDTDRDRYLACLLSPVEKRHSLAALYAFYAEIA
                     RVRDMVKEPLPGEIRLQWWRDILENEKSDGGGHPLAEALLRCVREHQLPVRVLQDMIDA
                     RIFDLYEDPMQDRAALEGYAGETASALIQLSSLILDPVNAAESADAAGHAGVAQTIAGL
                     LLLLPVHVGRGQVYLPAEILSATGLDREALLAVNDEGAVENAIRAFCGLGRDHLARARK
                     SASSISARNFSAFIPVALAEPVFDRAEAAGARILRQPLQAPQWQRQWRMWRASRRRKF"
     gene            complement(10865..11251)
                     /locus_tag="BMJ25_RS04020"
                     /old_locus_tag="BMJ25_04010"
     CDS             complement(10865..11251)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529512.1"
                     /locus_tag="BMJ25_RS04020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BMJ25_04010"
                     /product="Mth938-like domain-containing protein"
                     /protein_id="WP_033043008.1"
                     /transl_table=11
                     /translation="MAKGIEMRDAHFPGRAPVDAYGNGGFRFADMSHRGSVLMLPSGIY
                     AWGVAEGDLLAVAKFQRVFDEAPGIEVLLVGTGLEIRPLPVELKAALRAAGISSDPMST
                     GAAVRTYNVMLAESRAVAAALIAV"
     gene            complement(11254..13830)
                     /gene="secDF"
                     /locus_tag="BMJ25_RS04025"
                     /old_locus_tag="BMJ25_04015"
     CDS             complement(11254..13830)
                     /codon_start=1
                     /gene="secDF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013844336.1"
                     /locus_tag="BMJ25_RS04025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BMJ25_04015"
                     /product="protein translocase subunit SecDF"
                     /protein_id="WP_101806327.1"
                     /transl_table=11
                     /translation="MPRFSPFKNILIWLVVLAGFACALPNLLSREQLADWPTWLPHRQV
                     PLGLDLSGGSHIVLQVNREDIVAERLHSTIDAIADALRQADIHYTGLSGSGRSLQFRLR
                     DGTKATAAREALKALSAPVQQDGLFGKSIQELVYEESATNDTLRLRLTDEGIDFRVSAA
                     VARSVEVVRRRVGEVIAVAPSIERRENDRLSVQVPGLDEPQRLKDLLAQRGDVALRWLD
                     LSMPAQQAVDTRPPAASEVLYSLDDPPIPYLVERRAFAAAPDFVEAQPGFDPATAEPVV
                     DVRIAAGATERIAPILQSDPRKQFAIVLDGQVISTSVLDSAVSGDAVRISGNLSEDGAN
                     DLAALIRAGPLPVSLTVIEERTVGPEDGADAVANGLTAGLIAALAVAACMIGFYGFFGL
                     VAVVAVIVNVVLIIAVLSVTGATLTLPGIAGIILTIGIAVDSNVLIYERLREEARSSDK
                     PLRRALDDGFERVLRSIVDSNLTIFIAGVILLSLGSGAFSGFAVTLAVGSLTTILSAHS
                     LTRRLIRGWYRRRRPHRLPRGIRTGFFSRADFRFMAIRNPVFILMTALSLASLVLLTSL
                     GLNMGADFRGGSVIELRARTGVADRADIRARLDELNLGEVQIEELGSARDVLIRIPSQE
                     AGDNAEQTAVGLVRAELEDQYDFRRVAVVGPGVSGELTRAGSLALAAAVLSVLLYVWLR
                     FGRRFALGAVVATLHDVLLTLGFLGLTGIEFNLASIAALLTIVCYSLSDTMVIYDRVRE
                     NLVRYRRMPLSVLIDTSINQTLSRTVLTSLTTMLALVALYLFGGSVLVQTFSATLIFGV
                     LVGTVSSIYVAGPLLILFNRRNSRLGGTEDPNNPDSPSKTEAKGAV"
     gene            complement(13992..14324)
                     /gene="yajC"
                     /locus_tag="BMJ25_RS04030"
                     /old_locus_tag="BMJ25_04020"
     CDS             complement(13992..14324)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /gene="yajC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015241187.1"
                     /locus_tag="BMJ25_RS04030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BMJ25_04020"
                     /product="preprotein translocase subunit YajC"
                     /protein_id="WP_011975343.1"
                     /transl_table=11
                     /translation="MFITEAFAQTAAPGGGGADILMSILPFLLIFVVMYFLIIRPQRAQ
                     MKRREELLKNIRRGDQVVTGGGIVGKVTKVVDDTELEVEIAEGTKVRVVRSGVSEVRVK
                     GEPVKE"
     gene            14661..15536
                     /locus_tag="BMJ25_RS04035"
                     /old_locus_tag="BMJ25_04025"
     CDS             14661..15536
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018011013.1"
                     /locus_tag="BMJ25_RS04035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BMJ25_04025"
                     /product="ATP-binding protein"
                     /protein_id="WP_011975342.1"
                     /transl_table=11
                     /translation="MPESKIDVLLDEIHRLSAALERIAGPAYAVNDWNEADCFVWAPAT
                     RHLQPVPKPNRIDLALISGVDHVRDILFDNTLRFAQGYPANNVLLWGARGMGKSSLVKA
                     VHAKVAHDADSALKLVEVHREDIATLPVLMEILKAAPVPVIVFCDDLSFDHDDTSYKSL
                     KAVLDGGVEGRPANVLLYATSNRRHLLPRNMMENEQSTAINPSEAVEEKVSLSDRFGLW
                     LGFYKCSQDDYLAMVDGYAQYFKLPVEPETLHAEALEWATTRGSRSGRVAWQFIQDLAG
                     RLRRQLDTPA"
     gene            complement(15940..17469)
                     /locus_tag="BMJ25_RS04040"
                     /old_locus_tag="BMJ25_04030"
     CDS             complement(15940..17469)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010969286.1"
                     /locus_tag="BMJ25_RS04040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BMJ25_04030"
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="WP_101806328.1"
                     /transl_table=11
                     /translation="MRKFESPQAGKSMIRLCAAILLAGVATGCSSDASRFGGLFSRSDD
                     IMTGSIPQGSSTMPRGDVASGDAAPSYGNSASVGQSYPAGGGYNAAAAPVSSARVASTP
                     MVVQRTSLHEPSATSGQQQVRTASLDSQAAALPRSQPQSGGARDIPSKGGWSASNAPTI
                     LVRQGDTVTVLARRFGVPEKEILRANGLKSSSQVEPGQRLVIPTFGAAGSAAKAAASGS
                     IADVEGGKRRPSPLPTDQREVAILPGQSQSREKNESRSDVAAGKLNSAGEGGGTGGYAV
                     KPGDSLNRIAKANGVSVAALKQANGLTTEAIRIGQKLNIPSASANTPATDAVVTASVSP
                     KKNEAKVAATEQSKPAEAKAAAAKESVSEVAIKSDVNEDLPKSTGIGKYRWPVRGAVVA
                     AYGANVDGNRNDGINISVPEGTPIKAAENGVVIYSGSSLKELGNAVLVRHDDGTVTVYG
                     NAAELKVQRGQKVQRGQTLASSGMTGRASRPQVHFEVRKNATPVNPVTYLE"
     gene            complement(17705..18355)
                     /locus_tag="BMJ25_RS04045"
                     /old_locus_tag="BMJ25_04035"
     CDS             complement(17705..18355)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018097725.1"
                     /locus_tag="BMJ25_RS04045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BMJ25_04035"
                     /product="biotin biosynthesis protein BioC"
                     /protein_id="WP_011975340.1"
                     /transl_table=11
                     /translation="MQVGNRLNAAAAAGDGLGNLAGRPADQTGAASKSESGAQLPPASF
                     LDPTPRISLSADALLYLGRTKRTLEKLPPLTKDEWNNHLSPQLAAREHQAFGKFAQTAD
                     YRAYYRGFIDYYDNLRPEDQNSLRYFGTREAAVAGLRSLEYDVESGLDIGANFEGLVSV
                     FLEENKTALSPATAPPPAERAYFAWDASNISYEHEAPEPRLMTEIERLYSELI"
     gene            complement(18518..19171)
                     /locus_tag="BMJ25_RS04050"
                     /old_locus_tag="BMJ25_04040"
     CDS             complement(18518..19171)
                     /EC_number="2.1.1.77"
                     /GO_function="GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PCMT"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526709.1"
                     /locus_tag="BMJ25_RS04050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BMJ25_04040"
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="WP_011975339.1"
                     /sec_met_domain="PCMT (E-value: 3.2e-39, bitscore: 129.6,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNARVSQQEGFAAMVLRLRSGGIVNHELLKAVEQTPRLLFAPPQY
                     QDEVYSKRLIPLDCGSFMEGCDMAVRLLHCLNLKSGQRILEVGTGSGFTAAVMGRIAER
                     VLTIDRYQTLVASAQKNLEKAGIRNVVVRQADGSAGVPGEGTFDRILITAAFNSLPRMF
                     SDHLVSGGTLLVPIMMSETHCRIVRVNRTGSRFDREDLFDAPYLPIVPQVASFL"
     gene            complement(19168..19938)
                     /gene="surE"
                     /locus_tag="BMJ25_RS04055"
                     /old_locus_tag="BMJ25_04045"
     CDS             complement(19168..19938)
                     /EC_number="3.1.3.6"
                     /GO_function="GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="surE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010969283.1"
                     /locus_tag="BMJ25_RS04055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BMJ25_04045"
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="WP_011975338.1"
                     /transl_table=11
                     /translation="MRILLTNDDGIHAEGLSVLERIARTITDDVWVVAPEVDQSGLAHS
                     LTLSEPLRLRPVSERRFALRGTPTDCVIMAVKKILDRKPDLVLSGVNIGANLADDVTYS
                     GTVAGAIEGTLQGIRSMALSQAYHYAVGREVPWDVAETHAPALIRTLMDVDLPDGTLVN
                     LNFPNCAVDSVAGVEVTSQGKLDFGLSIDERTDGRGFPYFWLRFGERAGDFRSGTDIRA
                     LRDNKISVTPLKLDMTDHAVQDLIARALRKGTVA"
ORIGIN
        1 atgggtcaga tcctcgtgcg gccgaagtcc ggaaagatcg aggacgcggc actggctctg
       61 gaaaagctcg tgccggtgga cgtgctcagc ctcgcggaag gccggcaacg ctatggcctg
      121 ttcaccaatg cgacaggcgg cattctcgac gacctgatga tcgtcaatcg cggcgatcat
      181 cttttcctcg tcgttaatgc agcctgcaag gacgccgatt tcgcgcatct caagaatggt
      241 cttggcagtc tctgcgacgt gaccatgttg accgaccgcg ccctgattgc ccttcaaggc
      301 cctcgggcgg gggcggttct ctgcgaactg tgggcagacg tcgcgtcgat gcgcttcatg
      361 gatgtcgccg aagccgacct tcacgacgta agctgcgtca tatcccgctc cggttacacc
      421 ggcgaggatg gcttcgagat ttccattccc accgaagcgg ccgttgatgt cactcaacgt
      481 cttctcgaac accccgacgt cctgccgatc ggcctcggcg cgcgcgactc gctccgcctc
      541 gaagcgggcc tttgtctcta cggcaacgat atcgacacga gcaccagccc tatcgaagcg
      601 ggactcgaat gggcaatcca gaaaagccgc cgcgccgacg gtgagcgcgc aggcggcttt
      661 cccggcgccg acagaattct ggccgagttc acccacgggg tttccaggcg gcgtgtcggc
      721 ttgaagcccg agggccgtgc gcccgttcga ggtggcgtgc ggctatttgc cgatgcggaa
      781 ggcaacacgg cagcaggaac ggtcacctcc ggcggttttg gcccgagtgt cgatggaccg
      841 attgccatgg gctatgtcga cgccgaacac gcggaaaccg gtaccagact gtttgcggag
      901 gtgcgcggca agtttctgcc cgtcgccgtc tccgccctgc ccttcatcaa acaaacgtat
      961 aaacgctgat caggagaacc tgcatatgct taaattcacc gaggaacatg agtggctgaa
     1021 gatcgatggc gatgtcgcga cggtcggcat caccgagcat gcagcgggcc agctcggcga
     1081 tcttgtcttc gtggaactac cggaagccgg gagcaccttc tccaagggcg atacggcagc
     1141 gaccgtcgag tcggtcaagg cggcatcgga cgtctattgt ccgcttgacg gcgaaatcgt
     1201 cgaggccaac caggcgatcg tcgacgaccc ctcactcgtc aacagcgatc cgcaaggcgc
     1261 tgcctggttc ttcaagctga aactcgccga tccggacgcc gccaaggcgc tcctggacga
     1321 agcggcctat aaggagctcg tcgcctgatg agcatgccca aggactttac ctttaccgac
     1381 tacaagccct acgacttcgc caatcgccgt cacatcggcc cctcgccggc cgagatggac
     1441 gaaatgctga aggtcgtcgg ctatccgagc ctcgatgcgc tcatcgacga caccgttccg
     1501 ccgtcgatcc ggcagcagac tccactcgcc tggggtgcgc cgatgacgga acgcgaggcg
     1561 ctcgacaagc tgcgcgaaac ggcgaaccgc aaccggaagg tcgtttctct gatcggccag
     1621 ggctattacg gaaccatcac gcccccggtc atccagcgta atattctcga gaatcccgcc
     1681 tggtacaccg cctatacgcc ctatcagccg gagatcagcc aggggcgtct cgaagccctg
     1741 ctcaattttc agacgatggt ttgcgacctg accggacttg acgtcgcaaa cgcctcgctg
     1801 ctggacgagg cgacggcggc ggcggaatcg atggccattg cggagcgcgt cgcaaagtcc
     1861 aaagcgaagg cctttttcgt cgacgagaac tgccaccccc agacgatcgc actgttgaag
     1921 acccgggcag agccgctcgg ctggcagatc gtcgtcggca atcccttcga ggatctggac
     1981 gccgcaagcg ttttcggcgc gattttccag tatcccggca cttatggtca tgttcgcgat
     2041 ttctcgggcc tgatcgccag gctccacgaa ctgggcgcaa tcgccgccgt cgcagccgat
     2101 ccgctggcgc tggcgctttt gaagtcgccg ggtgagatgg gcgcagacat cgccgtcggc
     2161 tcaacccagc gcttcggcgt tccggtgggc tatggcgggc cgcacgccgc ctacatggcg
     2221 gtcagggacg cctacaagcg ctcgatgccc ggccgtctcg tgggcgtgtc ggtcgacgcg
     2281 cgcggaaacc gcgcctatcg gctttcgctg cagacgcggg agcagcatat acggcgcgaa
     2341 aaggcgacat ccaacatctg taccgcgcag gtgctgcttg ccgtcatggc ctccatgtat
     2401 gccgtcttcc acgggcccga gggcatcaag gcgatcgccc agagcgtcca ccagaagacg
     2461 gtccgccttg cgctcggcct cgaaaaactc ggctataccg tcgagccgga cgtcttcttc
     2521 gatacgatca ccgtcgaggt cggcaaactg caaggcatca tcctcaaggc tgccgtcgcc
     2581 gaggacgtga accttcgcaa gatcgggacg acgaagatcg gcatcagtct cgacgagcgc
     2641 tcccggccaa tcaccctcga ggccgtctgg cgggctttcg gcggcgattt cagcgtcgat
     2701 cagttcgagc cggactaccg cctgccgaag gaattgctgc gcaccagcga ctatctcacc
     2761 catccgatct tccacatgaa ccgggctgaa agcgagatga cgcgttacat gcgacgcctc
     2821 gccgatcgcg atcttgcgct cgatcgggca atgatcccgc tcggctcctg cacgatgaag
     2881 ctcaacgcga cggcggagat gctgccaata acctggccgg aattctcgga gatccacccg
     2941 ttcgtgcccg ccgaccaggc gatgggatat caccatctga tcgaagacct gtcgcagaaa
     3001 ctctgcgcga tcaccggcta cgacgcgatc tccatgcagc cgaactcggg cgcccagggc
     3061 gaatatgcag gtctgctcgc tatccgcgcc taccatattg ccaatggcaa cgaacatcgt
     3121 gacgtctgcc tgatcccgac ttcggcgcac ggcaccaatc ccgcctcggc gcagatggcc
     3181 ggcatgaagg tggtggtcgt caaggtgagc gacgccggcg agatcgccat ggacgacttc
     3241 cgcgccaaag ccgagcaata tgcggaaacg ctctcctgct gcatgatcac ctatccctcg
     3301 acccacggcg tattcgagga gaacgtgcgc gaggtctgcg agatcgtcca taagcatggc
     3361 ggccaggtct acctggacgg cgccaacatg aatgcgatgg tcggcctttc ccgtcccggc
     3421 gatatcggct cggatgtcag ccacttgaac ctgcacaaga ccttctgcat tccgcatggc
     3481 ggcggcgggc ccggcatggg tccgatcggg gtcaaagccc acctcgcacc cttccttccg
     3541 gggcatccgg aaagcggcga acatgagggc gccgtttccg ccgcaccgtt cgggtcggct
     3601 tcgatcctgc ctatctcctg gagctattgc ctgatgatgg gcggcgaagg cctgacgcaa
     3661 gcgaccaagg tggcgatcct caatgccaat tatgtcgccg cccggctgaa gggtgccttt
     3721 gacgtgctct acaagtcggc caaaggccgc gttgcgcatg aatgcatcat cgatacccgc
     3781 ccccttgccg agagcgccgg tgtgaccgtt gacgacgttg ccaaacggct ggtcgactgc
     3841 ggtttccatg cgccgacgat gagctggccg gttgccggaa ccttgatgat agagccgacg
     3901 gaatcggaga ccaaggccga gctcgaccgc ttctgcgatg ccttgctggc gatccgagag
     3961 gaagcccgcg ccatagcgga aggccggatg gacaagatca acaatccgct gaagaacgcc
     4021 ccgcatacgg tcgaggacct cgtcggcgac tgggaccggc cctattcgcg cgagcaagcc
     4081 tgcttcccgc ccggcgcatt ccgcgtcgac aaatattggt cgccggtcaa ccgcgtcgac
     4141 aatgtctatg gcgaccgcaa tctcgtctgc acctgcccgc cgatcaaaag ctatgcggaa
     4201 gctgctgaat gagatgatcg gaccgggcgc tggtcaggcg tccggctcga cgatcgtctc
     4261 ttggcgtaga ggacaccagg cgcgttcggc ccctcatccg cctgccggca ccttctccca
     4321 tcgggagaag ggacttgccg caaccgctca atactccgca acggctcgga agcaacagga
     4381 gcatgccgct tgtcccttcc ccccgctcgc ggggagaagg tgccggcagg cggatgaggg
     4441 gcgttcgctc cacatcagtt cagcgcgtcg acggcgcgag agctcccagc aagcgcggcg
     4501 aacgtcaggg ttgggcgaac gtcagggttg gcgcccggca tcagcgcata gaaaaagccg
     4561 cccggagggc ggctttttca attcattgca agacactcga tttactcggc gctctcgtcc
     4621 gaagcctttt tcttggccgg agccttcctt ttcggcgctg cggcttcttc gccctcggcg
     4681 gcatcggcct tggcagctgc cttcttcttg gaagccgtct tcttggccgg tttttcgtcg
     4741 gcgtcgtctt ccgcggtcag ctcttccttg gacaccttct tgtcggtcac cgagacctcg
     4801 gtgagcagat ggtcgacgac cttctcctcg aagagcggtg cgcgcagaga ggcggcagcg
     4861 ccgggcgtat tgcggaaata ttcgatgatt tccttttcct ggccggggaa ctgacgcagc
     4921 tgctggaaca gtgaattctg catctcgtca tcgctgacct ggacaccggc cttctcgcct
     4981 atttcggaga gaaccagtcc aaggcggacg cgacgctcgg caagcttgcg gtattcggcg
     5041 cgggcttcct cttccgtcgt gtcttcatcg gcaaaggttt tgccggcctg ctgcagatcg
     5101 gtgttgatct ggcgccagat gttctcgaat tcggcgtcga cgaggcgctc gggcgtatcg
     5161 aactggtaga gttcgtcgag ctggtctaga agctggcgct tgaccttctg gcgggttatc
     5221 gagccgaact ggctttcgat ctggccgcga acgatttcct tcagcttgtc gacggattcg
     5281 aggccgagct tcgttgccag ctcatcattg atctcgatcg gagccgcggc agcgacgtcc
     5341 ttcaccgtga cgtcgaaggt tgcttccttg ccggcgagat tggctgccgg gtagtcggcc
     5401 gggaacgtga ccgtgatggt cttttcatcg ccggccttcg cgccaacgag ctgctcttcg
     5461 aagccgggga tgaagcggtt ggagccgagc acgagttccg catcctcgtc cttgccgccg
     5521 tcaaaggctt cgccgtcgac cttgcccagg tagtcgatcg tgacgcggtc accattggcg
     5581 gccttgccct tcttcgattc ataagtacgg gcgctttcag cgatgcgctc gatctgctcg
     5641 ttgacttcgt cttcgccgac atcaacgact tcgcgagtga ccttgatgcc gctcgcgtcc
     5701 ttgagttcga tcggcggaat gatttcgtag gcgacggtga attcgaaatc cgcttcggcc
     5761 ttcaggatct tgtcagcttc cgcctcgtcc tcggtcatcc cgatttccgg ctgggtcgca
     5821 gacttctcgc cgcggctggt gaggatttcc gtcggctttt cacgaacgat ctcgttgacg
     5881 agatcggcca tgatcgactt gccgtaaacc ttcttcagat gcgcaaccgg caccttgccg
     5941 gggcggaagc cattgatgcg aacacgatcc ttcacctcga cgagacgctc gttcatccga
     6001 gcttccattt cgtcggccgg aatgacgacc ttgagttcgc gcttcagccc ttgagcgagc
     6061 gtttcgataa cctgcatgtt caaaccttca cttcacgttg acggtgctct tcccttgata
     6121 aggctaggcg agcacgtgag ccgatccatt tgccgggagt ggctttacgc aattccgacc
     6181 gctttgcaag caaaatggcg ccttgccggc cttcctcagc gccgaaaacg acgcgtgact
     6241 gctatatcgt cctggtgcgg gtagagagac ttgaactccc acgccttgcg gcaccagaac
     6301 ctaaatctgg cgtgtctacc aatttcacca tacccgcatt cagacgatcg caccgcccaa
     6361 tcgcattcct cacaagatct caccagcgga atgccgcaga tatttcttgg gccgtgcgaa
     6421 aggttcaggg gcttcccgag cgcggcgtct ctatatcact cgttttcatc ggatcaaagg
     6481 gaaaatgctc gcgcccgttg cccttatccg gcttgtctcc gaacgggagg attgccctct
     6541 ttacagccgg gtgccgacag aaacgcgatg cggcggcgca tgccgatgcg ttgcgcaaac
     6601 tgcatatctt ccatgtggac attgcactgc acaaatcgat ttaagcgtcc tatattcacc
     6661 tcatggaatt cgaagcagcg cactcctcct cccagcgccg ctttgatagg attggcagca
     6721 ctcctcctcc cggttgccaa tcgcctttgt gacgcccgcc ggtcctcccc cggcgggcgt
     6781 tctcttcctc tcccgcactg cactcttcaa gtgatccacc tgcctactat tttggcacat
     6841 gttcactttc cggctcctta gctatgcact gttcgcatag gtgccattcg gagagacccc
     6901 taccaagcta cgggtatgct gcctatattc cactcatcga tcgggcagcg cactcctcct
     6961 cccagcgcac ccgatacgga ttggcagcac tcctcctccc agttgccaat cagaacaagc
     7021 gacgcctgct ggacctcctc cccagcgggc gttgtttatt tctgctttct tattccatta
     7081 cagctacacc gcgcatctgc gctaaacgcg gcgcctctca gaagcgcgca aaagctcgct
     7141 gcaaaaattt ggaaggcgga ccggcaccaa aaggttaagt cggaattaat atcggacaaa
     7201 gctttgcaag acatgcatgg gtgcgatgca gcattatctc tgtaattttg ggtcaaatcg
     7261 attatattcc gggtcatcag atgatggcca gcaagagaga tagagcgctg gcgaacgacc
     7321 tagaaaggaa taggatcatg aacctcgcac gttctttcaa caactggcgc aagtatcgtc
     7381 agacctgcaa cgaactcggc cgcatgagcg atcgggaact gactgacctc ggcatcggcc
     7441 gggcagacat tccctacgtt gcgcgtcagg caatcaagta agcagaatag cttttcgcca
     7501 gatcttggcc tttcaaacgc cttccggctc tgccggcggg cgtttttctt tatctgctgc
     7561 tttttggtct catgcagtga ccccgggagt ttgaatctca agcgatggcg cattttcagc
     7621 cgaggctgta ttcatgctca tcaaatgatc gatgcactgc acaaatgcat agcagatgca
     7681 tctactttgc actccatctc ccaattagac aggcgtataa gaacctcaac gacgcagaca
     7741 atcactgtcg cgagcgatct tcttgaggaa acgaccatga acccgattcg cattgcaaag
     7801 agctggatca actaccgccg caccgttgcc gaactcggca gcctttcgaa ccacgccctg
     7861 aacgatatcg gcatcactcg ctacgatatc cgcaacatcg cggcccgctc cttccgttaa
     7921 ctggacgagc attgcacagc ttgaaaacgg cgccctcggg cgccgttttc gtttcttgga
     7981 agcgaagatc ggctgtggta ggaagccgcg catgaacaaa ctcacatcct cctattcccc
     8041 cgtccacgtc attggcggcg gccttgccgg atcggaagcc gcctggcaga tcgcggaagc
     8101 gggcgtcccg gtcatcctgc atgagatgcg cggcgtgcgc ggcacggacg ctcacaagac
     8161 cgacagcctt gccgaactcg tctgctccaa ttctttccgt tccgacgatg cgaccagcaa
     8221 tgccgtcggc gttctccatg cggagatgcg cctcgcgggg tcgattatca tgcgatgcgc
     8281 cgatctcaat caggttcctg ccggcggcgc gctggccgtt gatcgcgaag gcttcgccga
     8341 ggctgtcagc gcggcgatcg cggcccatcc gctgatcacc gtcctgcggg aggaaatccg
     8401 cggcctgccg ccgaaggaat gggatctcgc catcattgcg accggtccgc ttacggcacc
     8461 ggatctcgcc gacgcgatcc gggcggaaac cggcgctgac gccctcgcct ttttcgacgc
     8521 catcgcgccg atcgtccatg cggatacgat agacatggat atctgctggc atcagtcgcg
     8581 ctacgacaag gtaggccccg gcggcacggg caaggattac atcaactgtc cgctgacgca
     8641 ggaggaatac aacgccttcg tcgatgcgct catcgccggc gacaagaccg gcttcaagga
     8701 atgggaaggc acaccctatt tcgacgggtg ccttccaatc gaagtgatgg cagaacgcgg
     8761 acgtgagacc ctgcgtcacg ggccaatgaa gccgatgggg cttaccaacg cccataatcc
     8821 gtcggtcaag ccctatgcgg tcgtacagct tcggcaggac aatgcgctcg gcacgctcta
     8881 caacatggtc ggctttcaaa cgaaactgaa atatggcgcg caaggagagg tcttccggat
     8941 gatcccgggg ctcgagaagg cagagtttgc aagactcggc ggtctgcacc gcaataccta
     9001 tatcaattcg ccgaccctgc tcgaccagtc gctgaccctc aaatcgcgtg cgggactgcg
     9061 cttcgccggc cagatcaccg gctgcgaggg ctatgtagaa agcgcgagta tcggacttct
     9121 ggccggccgc ttcgccgcgg cggaacgcaa gggcgcctct cccgccattc cgccagtgac
     9181 gaccgccttc ggcgctctcc tcaatcacat caccggaggg cacatcgtct ccgacgatga
     9241 accgggcaag cgctccttcc agccgatgaa tgtcaatttc gggctgtttc cgccgctcga
     9301 tcccggggcc ctcacaaggc cggagggggc gaagcgcttt cgcggcaagg aaaaggcaag
     9361 cgccaagaag caggcgatgg cctcacgagc gctgagcgac tgtgcaacct ggctcgacaa
     9421 aaatctattg atcggcgggg cagcgacccg ttgacgtcaa cggtcaatcc ggtccaggcg
     9481 ccgcgttgcc cgacgcttcc ttctcgagtt tcgtcctgaa actgccgagc agccatagcg
     9541 agacctcggc cgcctcggcc gcggtccgaa attcgaaagc attgtcgaga taggcgaaat
     9601 ccgggaagtg gacgatcaac ccgcgcccac tgtctgatgt attgacccgc accttgtccg
     9661 gttgaacgac atgacagaca taatgcgtgc catgcgactg tatgaagccc gccttaccgt
     9721 catggagacg caggaagatc gctgttccgt caacgcttga atgcagcgcc cggatggcct
     9781 cgtcgggaaa tgcccggccg aattccacga tcgccgaacc cgcgtccgga ttgtctgcat
     9841 cgcgctcggc gcgtgcgttc atcctgatat aaaacacgca gacggcggca atcgcagccg
     9901 ggaacagtaa ccaaagcatc gcgtgggtcc cttgagagcg acgggaatcc gatagaacaa
     9961 tgggagactt gcgccaggat gacggccgaa cggctatccg ctaaaatttc cgacgccggc
    10021 tcgcccgcca catccgccat tgccgctgcc attgcggtgc ctgcagtggc tgacgaagga
    10081 ttcgcgctcc ggcggcctcg gcacggtcga acacaggttc ggccagggcg acgggtatga
    10141 aggcggagaa attccgggcc gaaatcgaac tggcactctt gcgcgccctg gcaagatgat
    10201 cgcggccgag accgcaaaat gcgcggatgg cgttttcgac ggcgccctcg tcgttcaccg
    10261 caaggagagc ctccctgtcg agccccgttg cgctcaaaat ctcagcaggc aaatagacct
    10321 ggccacgccc gacatgaaca ggcagaagaa gcaggagacc ggcgatcgtc tgagcgacac
    10381 cggcatgacc ggccgcgtct gcggattccg ccgcgttgac cgggtcgagg atcagcgaag
    10441 agagctggat gagcgccgat gcggtttcac cggcatagcc ttcgagcgcc gccctgtcct
    10501 gcatcggatc ctcgtaaaga tcgaaaatgc gtgcgtcgat catgtcctgc agaacgcgaa
    10561 caggcagctg atgctcgcga acgcatcgca ggagcgcttc ggctaaagga tgccccccgc
    10621 cgtccgactt ttcgttttcg agaatgtcgc gccaccactg cagccggatc tcaccgggta
    10681 gcggctcctt gaccatgtca cggacgcggg cgatctcggc gtagaaggca taaagtgccg
    10741 cgagcgaatg ccgcttctct accggagaaa gcagacaggc caggtaacga tcgcggtcgg
    10801 tgtcccgcag ggtcgcgaga atttggtcat cggactgttg cacgccgagt ttccttggaa
    10861 cgcctcacac cgcgatcaat gccgcggcga cggcgcgcga ttccgccagc atcacgttgt
    10921 aggttcgcac cgccgcaccc gtgctcatcg gatcggacga gatgccggcg gccctaagcg
    10981 ccgctttcag ctcgaccggc aacgggcgga tttccaggcc ggttccgacg agcagcacct
    11041 cgattccggg cgcctcatcg aaaacacgct ggaacttcgc gaccgcaagc agatcgcctt
    11101 cggccacgcc ccaggcgtag ataccggaag gcagcatcag aacggagccg cgatgcgaca
    11161 tgtcggcgaa gcggaacccg ccattgccgt aagcgtcgac aggcgcccgt cccggaaagt
    11221 gcgcatcgcg catttcgatc ccttttgcca tgatcagacg gcaccctttg cttctgtctt
    11281 cgacggcgaa tccggattgt tcgggtcctc cgtgccacca agccgactgt ttcggcggtt
    11341 gaagagaatg agcagcggtc cggcgacata gatcgaagag actgtgccca caagtacgcc
    11401 gaatatgagc gtcgcactga aggtctgaac aagaacgctg cccccgaaca gatagagcgc
    11461 gacgagagca agcatcgtcg tcaaggacgt cagaacggtt cgcgagaggg tctgattgat
    11521 cgacgtgtcg atcaggactg aaagcggcat ccttcgatag cgcaccagat tttcccgaac
    11581 tcgatcataa atcaccatgg tatcgctcag cgaatagcac acgatcgtca gcagcgccgc
    11641 aatgctggcg agattgaatt caatgcctgt cagcccaagg aatccgaggg tcagcagcac
    11701 atcgtgcaag gtcgcgacaa cggctcctaa cgcaaatcgc cggccgaaac gaagccagac
    11761 ataaagcaga acgctcagca ccgcagcagc aagagcaagc gatcctgccc gcgtcagctc
    11821 cccggaaacg ccgggaccga ccaccgcgac acgccggaag tcatactgat cctcgagctc
    11881 tgcgcgaacg agaccaactg ccgtctgctc ggcattgtcg cccgcttcct gcgaggggat
    11941 acggatgaga acgtctcgcg ccgaaccaag ttcctcgatt tgaacctctc cgaggttcag
    12001 ttcgtcgagg cgggcacgga tgtcggcacg gtcagccacc ccggttcgcg cccgcagctc
    12061 gataacggag ccacctctga aatctgctcc catgttgagc ccgaggctcg tcaggaggac
    12121 cagcgaagca agcgacaaag ctgtcatcaa aatgaaaacc ggattacgga tcgccatgaa
    12181 acggaagtct gcccgactga aaaatcccgt ccgaatgccc cgtggcagcc ggtgcggccg
    12241 acgacggcga taccagccgc ggataagccg gcgcgtcagg ctgtgcgcgg atagaatggt
    12301 ggtaaggctg ccgacggcaa gcgtgaccgc aaacccgctg aaggcgccgg agccgagaga
    12361 gagcaggata acgcccgcga tgaatatcgt aaggttcgag tcgacgatgc ttctcaagac
    12421 gcgttcgaag ccatcgtcca gcgcccgtcg caacggcttg tcactgctgc gtgcctcttc
    12481 cctgagacgc tcgtagatga gaacgttcga gtcgaccgca attccgatcg tgaggataat
    12541 cccggcaatt ccgggcaagg tcagcgtggc gccggtgacg gacaataccg cgattatcag
    12601 tacgacgttg acgatgacgg cgacgactgc gacgaggccg aagaagccgt aaaagccgat
    12661 catacaggcc gcgaccgcaa gggcggcgat caggccggcc gtgaggccgt ttgcaactgc
    12721 gtcggccccg tcctccgggc cgacagtacg ttcctcgatg acggtcagcg aaacgggaag
    12781 cggaccggca cggatcaaag cggcgagatc gttggcacca tcttccgaga gatttccgga
    12841 aatccggacg gcatcaccag agacggcgct gtccagaacg gaggtcgaga tgacctgccc
    12901 gtcgagtacg atcgcaaact gcttccttgg atccgactgc aggatcggag caatccgctc
    12961 ggttgcgccc gccgcgatcc ttacatctac gacaggttcg gccgttgcgg ggtcgaagcc
    13021 cggctgtgcc tcgacgaagt ccggtgctgc agcgaaggct cgtcgctcca cgaggtaggg
    13081 gatgggtgga tcgtcgagcg aatagaggac ctcggaagct gccggcggcc tggtgtcgac
    13141 ggcctgctgt gccggcatcg acaggtccag ccacctcaga gcgacgtcgc cccgctgggc
    13201 aagaagatct ttgagccttt gcggctcgtc caaaccgggc acctgcacgc tgaggcggtc
    13261 attttcccga cgctctatcg atggtgcaac tgcgatgacc tcgccgacgc gccggcgcac
    13321 gacctcgacg gaacgggcaa ccgcggcgga gaccctgaag tcgatgcctt cgtcggtcag
    13381 gcgaagccga agcgtgtcgt tggtcgcgct ttcttcgtag accaattcct ggatggactt
    13441 cccaaagagg ccgtcctgct gaacgggagc ggacaaagcc ttcaacgcct cacgcgcggc
    13501 agtcgccttc gttccgtcgc ggagacgaaa ctgcaggctc cgcccgctac ctgacagacc
    13561 cgtatagtgg atatccgcct gcctcaacgc atcggcgatg gcatcgatcg ttgaatgcag
    13621 gcgctccgca acgatgtctt cgcggttgac ctgcaggaca atatgagagc cgccactgag
    13681 atcgaggccg agcggtacct gccggtgcgg cagccatgtc ggccaatcgg caagctgctc
    13741 cctggaaaga agatttggca gcgcacaggc aaagcccgcc aggacgacca gccaaatcag
    13801 aatgttcttg aacggcgaaa acctgggcat acgtcgaaat gctccatccg ccggtctcag
    13861 ccggtcgcga cccctgccct aatcaggtga ttttggcctc agacagatcc aaaatcaaac
    13921 cgtgatccat gccgatagca agcgcggatg cgggcgaaaa atccgcgcac gcttctcagt
    13981 cccgcgacgg gctattcctt gacgggctcg cccttcacgc gaacttcgga aacgccgctg
    14041 cggacgacgc ggaccttagt gccctcggcg atttccacct cgagttcggt atcgtcgacg
    14101 accttggtca ccttgccgac gataccaccg ccggtgacga cctgatcgcc gcgccggatg
    14161 ttcttcagca gttcttcgcg gcgcttcatc tgtgcacgct gcggccggat gatcaggaaa
    14221 tacatgacca cgaaaatcag caggaatggc aggatggaca tcagaatatc ggcgccgccg
    14281 cccccagggg ccgccgtctg cgcgaaagct tcggtaataa acatcgatca ctccttgagt
    14341 tcaattgcgc gcttgccccc gcggcaaacc tgcaggaata taggaaagcc gtgccgcaac
    14401 acaaatcacc tgcactcttc cccgtttctc ctgcctctgg cacaaattcc gcagcaggag
    14461 aaccccttgg ttgcaggctg ccggtctttt ccagcgtaaa ccgccgtgct accgagaaaa
    14521 acgctgccgg gcagcctcaa tggattcgac cggaagacga atgtcgcggc gtttcaagaa
    14581 tttgcctaag cctggcgctt gcccgtcatc gcgcgggtca actgccgatt ttgaagttca
    14641 gcctgccgga gaagtatgaa atgcccgaaa gcaaaatcga cgtcctgctc gacgaaatac
    14701 acaggctttc ggcggcgctg gagcgtatcg ccggaccggc atatgccgtc aacgactgga
    14761 atgaggccga ctgcttcgtc tgggctccgg cgacgcgcca tctccagccc gtaccgaagc
    14821 cgaaccgtat cgatctcgca ctcatttccg gcgtcgacca cgtacgcgac attctcttcg
    14881 acaataccct ccgcttcgcc caaggctatc ccgccaacaa cgtgctcttg tggggcgcgc
    14941 gcggcatggg caaatcatcc ctggtcaagg cagtccatgc aaaggtcgca catgacgcgg
    15001 acagcgcgct caaactcgtc gaagtgcacc gggaggacat agccactctg cccgtgctca
    15061 tggaaatcct caaggcggcg cctgtgccgg tgatcgtatt ctgcgacgat ctctcattcg
    15121 accatgacga tacgtcttac aagtcgctga aggcagtcct cgacggcggc gtcgaaggac
    15181 gtccggcaaa tgtcctgctc tatgcgacat ccaatcgcag acacctgcta ccccgcaaca
    15241 tgatggaaaa cgagcaatcc accgccatca acccctcgga ggccgtcgaa gaaaaggtgt
    15301 cgctgtccga ccgcttcggt ctttggctgg gtttctacaa atgcagccag gacgattatc
    15361 tggcgatggt ggacggatat gcccaatact tcaaattgcc ggtcgagccc gaaacgctgc
    15421 atgccgaagc tcttgaatgg gcgacgacac ggggatcgag gtccggccgc gtcgcctggc
    15481 aattcatcca ggatctggcc ggccgcctgc gcaggcagct cgacacgccg gcctgataca
    15541 cgcggggatg cgggcagaga accacgcccg cctttcctca ccccgcgcta gactaagcta
    15601 cccttcatgg attgacggcc cggatgccaa tggcttcgaa cggaccctca ttgcgcgtcg
    15661 cgatggcagc cgaaccgttc gcccgcgcaa tggcctcaat tgcaccatcc ggaaaagtca
    15721 cctgaacgcc tctccgctgt gcgtccgcca ctgcattttg ctagcggctt aggtcacttg
    15781 acttcgatca gatcgatgca gcggtagctg ccaccaaggc tgccgtagca aggctgcaac
    15841 gatcgaaaac gccgcgcggc ctgtcagccg cgcggcgttt tcgatcgtct cgtgtttgct
    15901 cttaagtccc gtacggttcg ggatacgcgc ctgctatacc tattcgagat aggtcaccgg
    15961 gttgaccggg gtcgcattct tgcgcacctc gaagtgcacc tgcggccgcg atgccctgcc
    16021 cgtcatgcct gatgatgcga gcgtctgccc gcgctggacc ttctgaccgc gttgcacctt
    16081 cagttcggca gcattgccgt agaccgttac ggtaccgtca tcgtggcgca ccagaacggc
    16141 attgccgagc tccttgaggc tgctgcccga gtagatcacg acgccgttct cagccgcctt
    16201 gataggcgta ccctccggaa ccgatatgtt gataccgtca ttccggttgc catcgacatt
    16261 ggcgccatag gcagcgacga ccgcgccacg gaccggccag cggtatttcc cgatgccggt
    16321 cgacttcggg agatcttcgt tgacatccga cttgatggcg acttcggaca cgctctcctt
    16381 ggcggcagcc gccttggctt cggccggctt ggactgctcc gttgcggcaa ccttggcttc
    16441 gttcttcttg ggcgagacag aagccgtgac gacagcgtcg gtcgctggcg tattggccga
    16501 agcgctgggg atattgagtt tttgtccgat gcggatggct tccgtcgtaa gcccgtttgc
    16561 ctgcttcaga gcagcgaccg agacaccgtt cgccttggcg atccggttca gcgagtcgcc
    16621 cggcttgacc gcataaccgc cagtgccgcc gccctcgcca gcgctgttga gcttgcctgc
    16681 ggccacatcg ctgcggcttt cgttcttttc gcgcgattgg gactgaccgg ggaggattgc
    16741 gacctcgcgc tgatcagtcg gcagcggcga cgggcgcctc ttaccacctt ccacgtcggc
    16801 gatcgacccc gatgcagccg ccttcgcggc gctgcccgcg gcgccaaacg tcggaatgac
    16861 caggcgctgg cccggctcta cctggcttga cgatttcaat ccgttcgccc tcaggatctc
    16921 cttctcgggg acgccgaacc gtctggcgag aacggtcacc gtgtcgccct gacggaccaa
    16981 gatcgtcggc gcgttggagg cactccaccc ccccttgctc ggaatatccc tggcgccccc
    17041 cgactgcggc tgcgacctcg gaagggcggc ggcctgcgag tcgagagaag cggtccgaac
    17101 ctgctgctgc ccggatgtgg cggacggctc gtggaggctc gtacgctgga cgaccatagg
    17161 cgtggatgcg acacgagcac tggagaccgg cgccgctgcc gcattgtagc cgccgccagc
    17221 gggataggat tgaccgaccg acgcgctgtt tccataggag ggcgctgcat cgccacttgc
    17281 gacatcgcct ctcggcatcg tactcgaacc ctgcgggatc gagcctgtca ttatgtcgtc
    17341 agaccgcgag aagagcccgc cgaagcggct ggcgtcagaa ctgcaaccgg tcgctacacc
    17401 cgcaagcaat atagccgcgc agagacggat catagacttg cctgcctggg gagattcaaa
    17461 tttacgcatg acgctttacc cactccgaac gcaactcgct gtagaatgat taaagcgcat
    17521 tagagttacc gcccggttaa gggcggcacg attttggcga aatgctaacc atagggcgtc
    17581 caaacaggcg ctttccagcc agatgcagca cgcaggacgc gaagatgaaa cgctggagcg
    17641 ggatgagaaa aatatcgcgg cctttccgcc cgcatccccc attggaacca atcacgttcg
    17701 tgctctagat cagctcggaa tacaaccgtt ctatttcggt catcagcctg ggctccggcg
    17761 cctcgtgctc atagctgatg ttggatgcat cccaagcgaa atacgcgcgt tcggccggcg
    17821 gcggcgcggt tgccggcgag agggcagtct tattctcttc gaggaaaacg ctgaccaggc
    17881 cttcgaagtt ggcgccgata tcgaggccgc tttccacatc atattcaagc gagcgcaaac
    17941 ccgcgaccgc cgcctcgcgc gttccgaaat agcgcaggct attctggtcc tccggccgaa
    18001 ggttgtcata gtagtcgatg aagcccctat aataggccct gtaatcagcg gtttgggcga
    18061 acttgccgaa cgcctgatgt tcgcgcgccg caagctgcgg cgaaagatgg ttgttccact
    18121 cgtcttttgt gagaggcggc aatttctcga gcgttctctt ggtccggccg aggtaaagca
    18181 gcgcatcagc ggaaagcgaa atccggggcg ttggatcgag aaagcttgcc ggcggaagct
    18241 gcgccccgct ctcgctcttg cttgcggcac cggtctggtc ggccggccgc ccggcgagat
    18301 tgcccaagcc gtctccagcc gcagcggcag cgttcaatct gttcccgacc tgcatatgcc
    18361 gtctccgccg ttcgcatgac gcgcgatgcg gaaggaatgc ccacacgcga catgatcatg
    18421 ctgtccggcc taggactgca agcttgcgcg gagctggagc gagttaagat tctattaaga
    18481 tcgtatggtg cgcttgttct gaacacgccg agccgctcta aaggaacgac gctacctggg
    18541 ggacgatcgg aaggtaaggc gcatcgaaca gatcttcccg gtcgaaacgg ctgccggtgc
    18601 ggttgacgcg aacgatccgg caatgcgttt cgctcatcat gatcggcaca agcagggtac
    18661 caccggaaac gaggtgatca gagaacatcc gcggaaggct gttgaacgca gcggtaatga
    18721 ggatgcggtc gaacgtgccc tcgcccggaa cgcctgcgct accatccgcc tgccggacga
    18781 cgacgttgcg gatgcccgcc ttctccaggt tcttttgcgc cgatgcgacc agcgtctgat
    18841 atcggtcgat cgtcagcacg cgctcggcga tccgccccat aacggccgcc gtaaagccgc
    18901 tgccggttcc gacctcgagt atacgctggc cggacttcag gttgagacag tgcaaaagcc
    18961 ggacggccat gtcgcagcct tccatgaagg aaccgcaatc gagcgggatc agccgcttgg
    19021 agtagacttc gtcctgatat tgcggcggag cgaaaagcag ccggggcgtc tgctccacgg
    19081 ccttcagaag ctcatggttg acgatgccgc ccgagcgaag ccgcagcacc atcgctgcaa
    19141 acccctcctg ctgggaaacc cgtgcgttca agcgaccgtc cctttccgca atgcgcgcgc
    19201 tataagatcc tgaacggcat gatcggtcat atctagttta agcggggtta ccgatatctt
    19261 gttatcccgc aacgcccgaa tgtcggtgcc ggaacggaaa tcgccggcac gctcgccgaa
    19321 acgcagccag aaatagggaa agccgcgtcc atccgtccgc tcgtcgatgc tgagaccgaa
    19381 atcgagcttg ccttgcgatg tgacctcaac tcccgcaacg gaatcgacgg cgcaattcgg
    19441 gaagttcaaa ttgacaagcg ttccatccgg caggtcgaca tccatcaatg tgcggatgag
    19501 agccggcgcg tgcgtctccg ccacatccca gggaacctcc cggccgacgg cgtagtgata
    19561 tgcctggctg agggccatcg agcgaatccc ctgcaacgtg ccttcgatcg cgcccgccac
    19621 ggttccggaa taggttacgt cgtctgcaag gttggcgccg atattgacgc cggaaagaac
    19681 gagatcgggc tttcggtcga ggatcttctt caccgccatg atcacgcaat ccgtcggtgt
    19741 gccccggagc gcaaaccgcc gttctgaaac cgggcgcagg cgcaagggct ccgaaagcgt
    19801 cagcgaatgg gcaagcccgc tctggtccac ttccggcgcg acgacccaaa catcgtcggt
    19861 tatcgttcga gcgatccgtt cgagcacgga aagaccttcc gcgtgaatgc catcgtcgtt
    19921 cgtcagcagg atgcgcattg ccggtcttct tccatttatg gtgccggagc acttcccgga
    19981 aaaccgtcta acgggttctc tgctccggca ggtctcgatt tcacaaagct gggcctgccc
    20041 taagcagtct tctcgatccg tcgcaggccg cccatgtagg ctatgagggc gtctggcacg
    20101 gtgatagagc cgtcctcatt caggtaattc tcgatgacgg cgatcagcgc ccggccgagg
    20161 gcgacgccgg aaccgttcag cgtgtgggcg aacttcgtcc ccttttcttc ttttccgcgg
    20221 taacgcgcgt tcattcggcg gccttgaaaa tcgccgcaga ccgagcacga agaaatctcg
    20281 cgataggcgt cctgtcccgg cagccagacc tcgagatcgt acgtcttgcg agcgctaaat
    20341 cccatgtcgc cggtgcaaag cgtcatcaca cggtaatgca ggccgagacg cttaagtacc
    20401 tcctcggcac aagcagtcat ccgctcgtgt tcgtcgagcg cgctctcggc atccgtgatc
    20461 gacaccagtt cgactttatt gaactgatgc tggcgcaaca tacctcgggt gtcacggccg
    20521 gcggagccgg cctcggagcg gaagcatggg gtcaaggccg taaagcgcag cggcagcttt
    20581 tcgccgtcga ggatctgttc ccggaccatg ttcgtcagcg ggacttcagc cgtcgggatc
    20641 aaccagcgtc catcagtcgt gcggaagaga tcatcagtga atttcggcag ctggccggtg
    20701 ccatacatcg catcgtcgcg gacgagcaac ggaggctgaa cctcgatgta cccatgttcc
    20761 cctgtgtgta ggtcgagcat gaactggccg agcgcgcgtt ccaggcgcgc gagctgacct
    20821 ttgaggatcg tgaagcgcga ccc
//
