LOCUS       NZ_CP021011            10852 bp    DNA     circular CON 17-JAN-2024
DEFINITION  Bacillus velezensis strain GFP-2 chromosome, complete genome.
ACCESSION   NZ_CP021011
VERSION     NZ_CP021011.1
KEYWORDS    GSC:MIGS:2.1; RefSeq.
SOURCE      Bacillus velezensis
  ORGANISM  Bacillus velezensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus amyloliquefaciens group.
REFERENCE   1  (bases 1 to 3975220)
  AUTHORS   Sun,C.
  TITLE     Complete genome sequence of Bacillus sp. GFP-2, an antibacterium
            strain isolated from shark gut
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3975220)
  AUTHORS   Sun,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-APR-2017) College of Life Science, Zhejiang
            University, 866 YuHangTang Road, HangZhou 310058, China
COMMENT     ##MIGS-Data-START##
            alt_elev                          :: not reported
            assembly                          :: not reported
            collection_date                   :: 01-Oct-2016
            country                           :: China
            depth                             :: Not reported
            environment                       :: Shark intestine
            investigation_type                :: bacteria_archaea
            isol_growth_condt                 :: 10-40degC; 1% NaCl; ph 7
            lat_lon                           :: not reported
            project_name :: Genome sequence of Bacillus sp. GFP-2
            sequencing_meth                   :: A pacbio RS II platform
            num_replicons                     :: 1
            Estimated size                    :: "3,975,220 bp"
            ref_biomaterial                   :: GFP-2 = CGMCC 13337
            trophic_level                     :: Chemoorganotroph
            rel_to_oxygen                     :: Aerobic
            Library reads sequenced :: The large smrtbell gdna protocol (Pacific
            Biosciences, USA)
            Assembly method :: De novo assembled using the PacBio hierarchical
            genome assembly process (HGAP)/Quiver software
            Estimated error rate :: 99.99 % (average reference consensus)
            Method of calculation :: Estimation from HGAP Assembly quality
            scores
            Assembly name :: HGAP Assembly version 2 (Pacific Biosciences, USA)
            finishing_strategy                :: not reported
            biotic_relationship               :: free-living
            ##MIGS-Data-END##
            ##Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.0
            Coverage                          :: 247.76
            Sequencing Technology             :: PacBio
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/17/2024 08:46:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,936
            CDSs (total)                      :: 3,818
            Genes (coding)                    :: 3,738
            CDSs (with protein)               :: 3,738
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 9, 9 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 9, 9 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 52 of 80
            Pseudo Genes (incomplete)         :: 49 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 31 of 80
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:25:35
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2321883
            Orig. end                         :: 2332735
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP021011.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10852
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="9"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10852
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10852
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5852](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5852
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            41..994
                     /locus_tag="B6257_RS11960"
                     /old_locus_tag="B6257_11895"
     CDS             41..994
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1011: transport
                     system permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016937258.1"
                     /locus_tag="B6257_RS11960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6257_11895"
                     /product="ABC transporter permease"
                     /protein_id="WP_003156332.1"
                     /transl_table=11
                     /translation="MKLRYLLLLLAVLACTSIFIGVEDLSPLDIFHLNKEQAGTLFDSR
                     LPRLVSIIIAGMSLSICGLIMQQISRNKFVSPTTAGTMDWARLGILISLMLFTSASPLV
                     KMSIAFIFALAGNFLFMKILERIKFNDAIFIPLVGLMLGNIVSSIATFIAYKYDLIQNL
                     SSWLQGDFSLVVKGRYELLYLSIPLVIIAYVYADKFTVAGMGESFSVNLGLKYKRVVNI
                     GLIIVSLIASLVILTVGMLPFLGLIIPNMVSIYKGDNLRSSLPHTALLGAVFVLFCDIL
                     GRVIIFPYEISIGLMVGVIGSGIFLYMLLRRKAYES"
     gene            984..1931
                     /locus_tag="B6257_RS11965"
                     /old_locus_tag="B6257_11900"
     CDS             984..1931
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1011: transport
                     system permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388263.1"
                     /locus_tag="B6257_RS11965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6257_11900"
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="WP_007410274.1"
                     /transl_table=11
                     /translation="MSHNKKITLLAALAMICIGFFLFYGLGNWEYSLPRRCFKVLAMML
                     TGGAIAFSTVIFQTITNNRILTPGILGLDSLYMLIQTVIVFLFGSMNIVMMNKNLNFVI
                     SVGLMVLLSLVLYQLMFKGEGRNVFFLLLVGVVFGTLFNSLSSFMQMLIDPNEFQVVQD
                     KMFASFNNINTDLLGFAGAVFLLTGIYVWRFTRFFDVLSLGREHAVNLGIDYDKLVKQM
                     LIVVAILVSVSTALVGPIMFLGLLVVNLAREFLKTYRHTYLIAGSVLISIVALVGGQFV
                     VEKVFTFSTTLSVIINFAGGIYFIYLLLKENKSW"
     gene            1925..2683
                     /locus_tag="B6257_RS11970"
                     /old_locus_tag="B6257_11905"
     CDS             1925..2683
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388264.1"
                     /locus_tag="B6257_RS11970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6257_11905"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_071392129.1"
                     /transl_table=11
                     /translation="MVEVKNVSKQYGGKVVLEKTSLSIQKGRLTSFIGPNGAGKSTLLS
                     IMSRLIKTDSGEITVDGQEIGSCKSNDLAKKISILKQTNQINIRLTIKDLVSFGRFPYS
                     QGKLTDEDWVHINQALSYMKLDDLKDKYLDQLSGGQCQRAFIAMIIAQDTDYIFLDEPL
                     NNLDMKHSVEMMKLLKRLVEDFGKTVVVVIHDINFASVYSDHIVALKNGRIVKEGPAEE
                     IIETSVLEDIYDMTIPIQEIDSQRIGVYFS"
     gene            2705..3649
                     /locus_tag="B6257_RS11975"
                     /old_locus_tag="B6257_11910"
     CDS             2705..3649
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1033: iron
                     compound ABC transporter, periplasmic"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234484.1"
                     /locus_tag="B6257_RS11975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6257_11910"
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="WP_003156329.1"
                     /transl_table=11
                     /translation="MKKFALLFIALVTIFVVSACGNGDKTSGKSKETITIKDDLNKEGV
                     KVPKNPKKVVVFNFGMLDTMDELGLQDHVAGLPKQNLPKYLSSYNSDKYANTGGLKEPD
                     FEKVADIDPDLIIIAHRQSDSYKEFSKIAPTIYLDDDYTNYVDRFKQNTEVIGKIFNKE
                     NEVKDKLAKIDKSIADLKKKTSSTDKNGLVVMANDGKISAFGSKSRYGFIHDVFGVKPA
                     DKNIEASLHGQSISYEYIAKTNPDYMFVVDRGTAIGTKSSTKQVVENDYVKSVKAVKNN
                     HVVYLDSATWYLSGGGLESMAQMVKEVKDGIEK"
     misc_feature    4177..4191
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 22.4"
                     /tool="antismash"
     misc_feature    4500..4514
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: weak, score: 16.7"
                     /tool="antismash"
     misc_feature    4501..4515
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 17.93"
                     /tool="antismash"
     misc_feature    complement(4857..4870)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.23"
                     /tool="antismash"
     gene            5001..5852
                     /locus_tag="B6257_RS11980"
                     /old_locus_tag="B6257_11915"
     CDS             5001..5852
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF016970.4"
                     /locus_tag="B6257_RS11980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6257_11915"
                     /product="DUF692 family multinuclear iron-containing
                     protein"
                     /protein_id="WP_101562699.1"
                     /sec_met_domain="DUF692 (E-value: 3.5e-28, bitscore: 96.3,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNYPHLGVGMLVHNSEVYMDYLEKLGDVSEVYELLLIQYLNEPDE
                     LNKMVDRLGKPIIAHTTRFSFGTDTPMDSKLLNSMNDMMLRCKAKWCGDHLCFTGVPGL
                     AAGGLLPPIGNEESIDIFVRNILEAKKVINTPLLIENVVEYFQAGTITHAEILKEVVER
                     ADIGIVLSMENISSSKMYSDCDYYEFIDTIPSDRIVQIHCTIGNSQQQKEMKSDIFGTV
                     YDKQLMQYKALEYLSKKSGVQPKAFIWELETETDSHPEVEELRERMNWAKSLFFKNKEV
                     VS"
     gene            5852..6649
                     /locus_tag="B6257_RS11985"
                     /old_locus_tag="B6257_11920"
     CDS             5852..6649
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B6257_RS11985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="B6257_11920"
                     /product="hypothetical protein"
                     /protein_id="WP_101562700.1"
                     /transl_table=11
                     /translation="MSTLQLQTFLTCYIRNNSLRERMKSQSTENILYEYDLSKEELKLI
                     ETLDFTQLNRTADHLEEERKGKRCADFSEFTSVLQQANLFDSFFSSFTQSYTDGFLTSE
                     IEAERFLEHAHKYISDNSLPDILADLATYSYQVFRISQVEIQDVYNKNKLNWNKPLFLK
                     RPFSKKTFSYNLMEIIDNENIKKIEDIETIQKKETTYLFQKDYSNLISSNIFEVDDSVF
                     FRLVEQNLTGYEILNRANSINEKSKWRDLISELYDLDIVGTTD"
     gene            6906..8702
                     /locus_tag="B6257_RS11990"
                     /old_locus_tag="B6257_11925"
     CDS             6906..8702
                     /EC_number="3.4.-.-"
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR02290.1"
                     /locus_tag="B6257_RS11990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6257_11925"
                     /product="M3 family oligoendopeptidase"
                     /protein_id="WP_101562701.1"
                     /transl_table=11
                     /translation="MTNQLNKYSLTWDLDAFFLGGSLSEDFKIYMNHIKTDIKKFELLV
                     SSSELKESRDLTIWRNILDIWQDLSCRLGEAEAYISCLIAQDMRDKEARNLKMNFSVIE
                     SAFNAALEKLDQLFLEMKKSDWEKMMNDDYFKQVSFPLKERREKIKELSSLDKENLIKE
                     LAIDGYHAWGDLYSNIISKITIPYTINGSSKLLSVGQAMNILDSSDRSIRQQIFHLLND
                     IWEREEDLIGSILNHLCGFRLNVYNQRGWSNPVKESLINNRISEETLLTMWDVINKNKK
                     GLIQFLERKAKIIGVDKLAWYDVFAPLGKTKSQMSYEDAVDTIVKKLGEFDMDLSVFAE
                     KVFQEKWIEAEDRPGKRSGAFCIYFSDSAQSRIFTTFSGSSSIVSVLAHEIGHAYHQYT
                     MRDLPKLVQNCGLCLSETASMLTELIVTDSTICETVSKEQKILLLEEKIRQAVSCLLGI
                     QARFLFETRLNNERKKGIVSAKRLKELTVQAQQDAFSDCLSEYHPWLWASRLHYYITEV
                     PFYNYPYTFGYLFSIGIYKRYSENKINFSNQFIELLKSTGSMTAEELALKYLGVDLTGP
                     EFWQDAIELIKDDINNFLELTE"
     gene            8751..10004
                     /locus_tag="B6257_RS11995"
                     /old_locus_tag="B6257_11930"
     CDS             8751..10004
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /locus_tag="B6257_RS11995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6257_11930"
                     /product="MFS transporter"
                     /protein_id="WP_101562702.1"
                     /transl_table=11
                     /translation="MDEKGLFKKNLNFRYIWFAQSGSTLGDWFNQVAITQTVLMLTNSP
                     AAVGMLLLCRSLPVVFLGPILSPLVDKWPKKVIMIATDILRALLVLSFIGAIVYNNVYV
                     LYINSFLFGVISVLFNPTKQALLPLIVNRKDLPEANAFMSSTDAIVNIIGAVLGGIVST
                     VFSPITCFLVNSLSFLWSAYYVFKIKYQENKKSDIQNQPYIQLLKEGLLESNQNILIRY
                     VILIGLTWGLVGGGYYILIPFLGNNIYHLGAVGIGALYAIDGLGILLGTYIVKKIIKNN
                     FKRALFSFGISYSVQSMLFVLLTHSTVFYLGSLFLFLMRVCGGVIIPLSNYFVQMGTTD
                     EIRGRVFSLYNSTYMGVMQISYFISGYAYEKYSVPIVGAIGGMVSMLCGLIWLLQVMKG
                     KFEGYRENNRTSQKFIDY"
     gene            10036..10716
                     /locus_tag="B6257_RS12000"
                     /old_locus_tag="B6257_11935"
     CDS             10036..10716
                     /EC_number="2.1.-.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /locus_tag="B6257_RS12000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6257_11935"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_101562703.1"
                     /transl_table=11
                     /translation="MKPDYVDNLEEMEKFFDEKAKDWDEYAKHDPYNHYFKAISSHVSE
                     TKEKIRILELGCGTGLTLHSILDKAPNAIITCIDLSENMLEVLSKKFSEYSSQIQIRHG
                     SFFEFPFGKEQFNYIISSQTFHHFTPNTKKALYEKIYLSLERGGKYIEGDFLVSIEEEK
                     ESLNEYYKYVAPLEEKEEGKYHFNIPLALETQKQLLSDSGFKWENDIYYEDETELAVYT
                     GLKE"
ORIGIN
        1 caatcagtca taccgtttgt gatgggtgca gggaggcagc atgaagctac gttacttact
       61 tttgctactg gcggttttgg cgtgtacttc tatatttatc ggtgtagaag atttgtcgcc
      121 gctggatatt tttcatttaa ataaagaaca ggcaggtaca ttgtttgaca gccggctgcc
      181 gcgtttggtg agcattatca tcgccggcat gagcctgagc atctgcggat taatcatgca
      241 gcagatcagc cggaataaat ttgtatcgcc gacgactgcg ggaacgatgg actgggcaag
      301 gctcggaatt ttgatttcat taatgctttt tacgtcagcg agtccgcttg tgaaaatgtc
      361 tatcgctttt attttcgcgt tggcgggaaa tttccttttc atgaaaatcc tcgagagaat
      421 caagttcaat gatgcgattt ttattcctct cgtcggttta atgcttggga atatcgtcag
      481 ctcaatcgcc acgtttatcg catataaata cgacctgatc caaaacctgt catcttggct
      541 gcagggagat ttctcactgg ttgtcaaagg caggtatgag cttttatatc tgagtatccc
      601 gctggtgatc atcgcttatg tctatgcgga taaatttacg gtggcgggaa tgggagagag
      661 cttttccgtc aatctcggcc tgaaatacaa acgcgtcgtc aatatcggtc tgattatcgt
      721 gtctctcatc gcttcactgg tgatcctgac cgtcgggatg ctgccgtttc tcggtttgat
      781 cattcccaat atggtgtcga tttacaaagg ggacaacctg aggagcagcc tgcctcacac
      841 cgctctcttg ggcgctgttt ttgtgctgtt ttgcgacatt ttgggaaggg tgatcatctt
      901 cccgtacgaa atttccatcg gcctgatggt cggtgttatc ggcagcggca tcttcctgta
      961 tatgctgtta aggagaaaag catatgagtc ataataaaaa aattacgctg ctggcggcgc
     1021 tcgccatgat atgtatcgga ttctttctgt tctacggact gggcaattgg gaatactccc
     1081 tgccgagacg atgcttcaaa gtgcttgcca tgatgctcac cggaggagcg atcgcgtttt
     1141 caacggtcat cttccagacg attacaaata accgcatact gacaccgggc attttaggcc
     1201 ttgattccct gtatatgctc attcagaccg tcatcgtgtt cctgttcggt tcaatgaata
     1261 tcgtcatgat gaacaaaaat cttaatttcg tcatctccgt cgggctgatg gtgctcttgt
     1321 cactcgtttt gtatcagctc atgtttaaag gcgaggggcg gaacgtgttt ttccttctgc
     1381 ttgtcggcgt cgtgttcggc acgctgttca acagtctgtc atcctttatg cagatgctga
     1441 ttgatccgaa tgagttccag gttgttcagg acaaaatgtt tgccagcttt aacaatatta
     1501 acaccgatct tttaggattt gccggtgctg tctttcttct gacaggcatt tatgtctggc
     1561 ggttcacgcg gttttttgac gtgctgtcgc tcggcaggga gcacgccgtc aaccttggga
     1621 ttgattacga taagcttgtg aaacagatgc tcatcgtcgt cgccattctt gtgtctgtct
     1681 ctacagctct tgtcgggccg attatgtttt taggactgct tgtcgtcaat ctggcgagag
     1741 agtttttaaa aacctacagg cacacgtatt taatcgcggg ttccgtcctg atcagcatcg
     1801 tcgcgctcgt cgggggccag ttcgtcgtgg agaaagtgtt cacgttttcc acgacgctga
     1861 gcgtcatcat taattttgcc ggcgggattt attttatcta tctgctgtta aaggagaata
     1921 aatcatggta gaggtcaaaa atgtaagcaa acaatacgga gggaaagtcg tccttgagaa
     1981 aacgtctcta tccatccaaa agggcaggct cacctcgttt atcggtccga acggcgcggg
     2041 aaaaagcacg ctgctgtcta tcatgagccg gctgattaag acggattccg gagaaatcac
     2101 ggtcgacggg caagagatcg ggtcctgtaa aagcaacgat ctggcgaaaa aaatcagcat
     2161 cctgaagcag acgaaccaaa tcaatatcag actgacaatt aaagacctgg tcagcttcgg
     2221 cagatttccg tattcccagg ggaaactgac ggatgaagat tgggtgcata tcaatcaggc
     2281 cctcagttat atgaagctgg atgacttaaa ggataaatac ctcgatcagc tcagcggagg
     2341 ccagtgtcag agggcgttta tcgcgatgat catcgctcag gatacggact atatttttct
     2401 cgatgagccg ctgaacaatt tagatatgaa gcattccgtt gaaatgatga agctgttgaa
     2461 gcggcttgtg gaagacttcg gaaaaacggt cgtcgtagtg attcacgata tcaacttcgc
     2521 atccgtctat tcagaccata ttgtcgcact gaaaaacggc cggatcgtca aagaagggcc
     2581 ggcggaggag atcattgaaa cctccgtgct tgaagatatt tatgatatga ccatcccgat
     2641 tcaagagatt gacagtcagc ggatcggtgt gtacttttct taaaaaccag atgaggtgaa
     2701 agtcatgaaa aaattcgcat tgctattcat tgcgctagtt acgatttttg ttgtttctgc
     2761 atgcggaaac ggggacaaaa catcaggcaa atcaaaagaa acaatcacaa tcaaagatga
     2821 tctgaacaaa gaaggagtca aagtgccgaa gaatccgaaa aaagtcgtcg tattcaactt
     2881 cggaatgctg gatacgatgg atgaactcgg tttacaggat cacgtggcgg gacttccgaa
     2941 acaaaacctt ccaaaatatt tatcttcata taatagtgat aaatacgcca ataccggcgg
     3001 tttaaaagaa ccggattttg aaaaagtcgc ggatattgat ccggatttaa tcattatcgc
     3061 ccacagacaa tctgattctt ataaagagtt ctcgaaaatc gcgccgacga tttatcttga
     3121 tgatgactat acaaactacg ttgacagatt caagcaaaac accgaagtga tcggcaaaat
     3181 ctttaacaaa gaaaatgaag tgaaagataa gcttgcgaaa attgacaaat ccattgccga
     3241 tctgaagaaa aagacaagca gcacggataa aaacggatta gtggtgatgg cgaacgacgg
     3301 caaaatcagc gcgttcggat ctaaatcaag atacggcttc attcatgatg tgttcggcgt
     3361 caaaccggct gacaaaaaca tcgaagcctc actgcacgga caaagcattt cctatgaata
     3421 tatcgccaaa acaaaccctg attacatgtt tgtcgttgac cgcggaacag cgatcggcac
     3481 aaaatcttca acaaaacaag ttgttgaaaa tgattatgta aaatcggtga aagccgtgaa
     3541 aaacaaccat gttgtctacc ttgattcagc tacatggtat ttatcaggag gcggcctgga
     3601 atccatggcg caaatggtga aagaagtaaa agacggaatc gagaagtaaa ttaaattttt
     3661 atctttttaa aaggcttgag gctgtcggac tgccctgatc tgagagcatc tttaaacagc
     3721 cagctgccga cagaagatcg gcgactggct gttttgttgt agaattaata ttcaatagtt
     3781 actctgcaat gactgtcgta atgttgagat agaatattca aacttactga tttactggtt
     3841 aatgaaatta tataaaagaa tcagttagag gctattggaa tgctaacttc gactgttttc
     3901 tgatactctt gagttggaat ttttacatgt gtcaggaatt aaccttccta cagaataggc
     3961 agcgaccgtt aaaataagga gtggtttcac agttagttca atctttatga aaagaaacct
     4021 gtcttgttac agtcctaatc agcaggaatt taaataatat cgtaggtatc tcattcctct
     4081 gtctttgtga acactacaaa aagtcttaat taaggggcaa cattgaaact cacgtgttta
     4141 gctcaatgtt cctttttttg gctgcctgtt tgttggtttt ttctgataat catgcttaat
     4201 caagagatac caagatcccg atacatgttg ttggcatcaa ttcagttcct cttactaatt
     4261 ctgcgactgt agacggtact gttccctctc cccatacttc atactttttt agaacttcag
     4321 tttaaccttt ccgaagttat ttcagatttc agttcctttg tttgtaaatt atccaaaagg
     4381 tatactagct tatccatctg ataagagtgg ataggagccc acggatgctt aattatttca
     4441 tccatgtttt gaaattttca tgcttgaatt ttaatttgat ttttaatctt tttaacaggc
     4501 gtgctgatcg gccattcttg ttgaatttct ttaaagcatc attgatcatt cttatttcat
     4561 gaaaaatgct accaaaattg ttttaaattc tccgttttca actagacggc attttatttg
     4621 aggaaagctt caggtgcata atagagaaga tttttcttag ttatggaaaa aataaatgtt
     4681 attaaaggct aaaggagaga aaagagaaag atcagacttt ttaagttcaa gaataattat
     4741 taatagtcct gttaaaagga taagacgaga ccttcagaag aaagcattaa attaaacata
     4801 aaaggttttt ttctctaact aactctgcat atacaaaaaa atgtagaata atgtagtttt
     4861 ttgtaaaaat atgaaaaaaa atgtagtaat aaaaatgttt aaaagtgtta aaatgtgtta
     4921 tataatccaa ctaaagggtt ttaatggatt tttaattttg tgaaatttat ttacaggaag
     4981 tattgggagg cctgtttaga atgaattacc cgcatttagg agtaggtatg cttgtccata
     5041 attctgaagt ttacatggat tatcttgaaa aattggggga tgtatccgaa gtctatgaat
     5101 tgttacttat tcaatactta aatgaaccag atgagttaaa caaaatggtt gatcgactag
     5161 ggaagcctat tattgctcat actacaaggt tttctttcgg aacagataca cctatggata
     5221 gtaagctgtt aaatagtatg aatgatatga tgctcagatg taaagcaaaa tggtgcgggg
     5281 atcacttatg ttttacaggt gttccaggct tggcagctgg tggtttactt cctcctattg
     5341 ggaatgagga gtctattgat atctttgttc gtaatattct tgaagctaaa aaggtaatca
     5401 atactccgct cctgattgag aatgttgtag agtattttca ggcaggaaca ataacacatg
     5461 cggagatcct gaaagaagta gttgagaggg cggatattgg aatagtgctc agtatggaga
     5521 atatcagcag ttcaaaaatg tattccgatt gtgattatta cgagtttatt gacactattc
     5581 cttctgaccg gattgttcaa attcactgta ccataggtaa cagtcagcaa caaaaagaga
     5641 tgaaatctga tatatttggc acggtttatg ataaacagtt gatgcagtat aaggctttgg
     5701 agtatctatc caaaaaaagc ggtgtacaac caaaagcgtt tatatgggag ctagaaactg
     5761 aaactgattc tcatccagaa gttgaagaat tgcgtgaacg gatgaattgg gctaaatcat
     5821 tgtttttcaa gaacaaagag gtggtctcgt aatgagtaca ctacaattgc aaacctttct
     5881 aacttgttat atcaggaata attctttacg ggaaagaatg aaatcacaat caactgagaa
     5941 tattctttat gaatatgatc tatcaaaaga agagttaaaa ttaatagaga ccctagactt
     6001 tactcaactt aatcggactg ctgatcattt ggaagaggaa cgaaaaggaa agagatgtgc
     6061 tgatttttcg gagtttactt cagtcttgca gcaagcaaat ctatttgatt cattcttcag
     6121 ctcatttacg cagtcttata ccgatgggtt tctcacatca gaaatagagg cagaacgctt
     6181 tttagagcat gctcataagt atattagtga taatagttta cccgatattc ttgcagatct
     6241 tgccacatat tcatatcaag tatttagaat atctcaagtt gagatacagg atgtatataa
     6301 taaaaataaa ctcaactgga ataagcctct ctttcttaaa cgaccattta gtaaaaaaac
     6361 tttttcttat aacttaatgg aaattattga taacgaaaat attaaaaaaa tagaagatat
     6421 agaaactatt caaaaaaaag agacaacata cttatttcaa aaagattact caaatttgat
     6481 tagttcaaat attttcgaag tagatgattc tgttttcttc agactagtgg agcagaactt
     6541 aactggttac gaaattctga atagggcaaa ttccattaat gagaagagta aatggagaga
     6601 tttaatttct gagttatatg atttggacat agtcggaact acggattgaa agtattactt
     6661 gaaagggggt gaaatcatga aaaaagtaca agtgcgtaaa aatgacggaa aagtcgaaaa
     6721 aaacagcaat aatggtaagc aataatctgt aataacaaag aaatatgcgg gggctgtccc
     6781 ccgcatattt tgtttagaat aacattatga aaaaaataat ggtatatttc aagagtttta
     6841 aggtatttta ctgcttattt tagtagttaa tcgtgggcca caaaggtaag tgaaagggta
     6901 gtgctatgac taatcaatta aataagtata gcttaacatg ggatttggat gctttcttct
     6961 taggaggaag cttatctgag gattttaaaa tctatatgaa tcatattaaa actgatataa
     7021 agaaatttga acttctagta agttcttctg aactaaaaga aagtagagat ctcacaattt
     7081 ggagaaatat tcttgatatc tggcaagatt tatcatgtcg tttaggagaa gcagaggctt
     7141 atattagttg tttgattgct caggatatga gagataaaga agcccgaaat ctaaaaatga
     7201 atttttcagt aattgaatca gctttcaatg cagcactaga aaagctagat caattatttc
     7261 tggaaatgaa aaagtctgat tgggaaaaaa tgatgaatga tgattatttt aagcaagttt
     7321 cttttccttt gaaggagaga agagaaaaaa taaaagagct ttcatcttta gataaagaga
     7381 atctaatcaa ggaactggct attgacggct atcatgcttg gggagatttg tatagcaata
     7441 taattagtaa aataacaata ccttatacga taaacggaag cagcaagctt ctatctgttg
     7501 gacaggcaat gaatatatta gacagctctg atcgatccat tcgccaacaa atatttcatt
     7561 tattaaatga tatttgggag agagaggagg atcttatagg atctattcta aatcatttat
     7621 gtggattccg tcttaatgta tataatcagc ggggatggtc aaatcctgtt aaagaatcat
     7681 tgattaataa tagaatttct gaggaaaccc tattaacaat gtgggatgta attaacaaaa
     7741 ataaaaaagg tctaattcaa tttttagaac gcaaggcgaa aattatagga gtggacaagt
     7801 tggcttggta tgatgtattt gcaccacttg gtaaaaccaa aagccaaatg agttatgaag
     7861 atgctgtaga caccatagtt aaaaaattag gggaattcga catggattta tcagtttttg
     7921 cggaaaaagt gtttcaagaa aaatggatag aggctgagga tcgtcccgga aaacgatctg
     7981 gagcattttg tatatatttt tctgacagtg ctcaatcacg tattttcacg acgttttctg
     8041 gtagttcatc gattgttagt gtgcttgccc atgaaatagg tcatgcatac caccaatata
     8101 ctatgcgtga cttacctaaa ttggtgcaaa actgtggatt atgcttatca gaaacagctt
     8161 caatgctgac tgaacttatt gttacagact ccacaatttg tgaaactgtg agtaaggaac
     8221 aaaaaatctt acttttagaa gaaaaaataa ggcaggcagt atcttgtttg cttggtatac
     8281 aagctcgctt tctttttgag acaagactta ataacgagcg gaaaaaaggg atagtcagtg
     8341 caaaacggct caaagagcta actgttcagg cacagcagga tgcctttagt gattgtctct
     8401 ctgaatacca cccttggctg tgggcttcta gacttcatta ttatattaca gaagtgcctt
     8461 tttataatta cccatataca tttggatatt tattcagtat tgggatttat aagagatatt
     8521 cagagaataa aataaatttt tcgaatcaat ttattgaatt gttgaagagt acaggaagta
     8581 tgactgcgga agagttggct ttaaaatatt taggcgttga tctaacaggc cctgagtttt
     8641 ggcaggatgc aatcgaatta atcaaggatg atatcaataa ctttctagaa ttaacagaat
     8701 agaaaaacaa tgtaataatt tcgaggttaa cgttaaaggt gtggtaataa atggatgaaa
     8761 aaggcttgtt taaaaagaat ttaaatttcc gatatatttg gtttgcgcaa tcgggcagca
     8821 ctttaggtga ctggttcaat caggtagcta tcacacaaac agtgctaatg ttaactaatt
     8881 ctcccgcagc agtaggtatg ttattgcttt gtcgttcttt accagttgtt tttttaggac
     8941 ctattttaag tccgctcgtt gacaaatggc caaaaaaagt aatcatgatt gctacggata
     9001 tcctcagagc tttgcttgtt ttaagtttta taggggctat tgtctataat aacgtatatg
     9061 tgttatatat taattcattt ttgtttggtg taattagtgt cttatttaat ccgacaaaac
     9121 aagcgttgtt gccactaatt gttaacagaa aggatttgcc agaagcaaat gcatttatgt
     9181 ctagtacaga tgcaatagtt aatatcattg gggctgtttt aggtggtatt gtatccactg
     9241 tatttagtcc aataacgtgt tttctggtta attccttgtc atttctttgg tcggcttatt
     9301 acgtttttaa aataaaatat caagaaaata agaaatctga tattcaaaat cagccctata
     9361 tacaactttt aaaagaagga ttattggaaa gcaaccagaa catcttgatc cgttatgtaa
     9421 tcttaattgg attaacatgg ggattggttg gtggaggata ttatattcta attccttttt
     9481 taggtaacaa catttaccat ttaggtgctg taggtatagg cgctttatat gctatagacg
     9541 gactgggtat tttgttaggt acttatattg taaagaaaat aattaaaaat aattttaaac
     9601 gtgcactatt ttcttttgga atatcttact cagttcaatc catgctgttt gtgcttttaa
     9661 cacactccac agtgttctac ttaggaagtc tatttttatt tttaatgaga gtatgtgggg
     9721 gggtcattat tcctctaagt aattattttg tgcaaatggg gacaaccgat gagatcaggg
     9781 gaagggtctt ttcattatat aattcaacct atatgggtgt aatgcaaatt tcatacttta
     9841 ttagtggtta tgcttatgag aaatatagtg tgcctattgt cggagcaata ggaggcatgg
     9901 tatccatgtt atgcggattg atatggttat tacaagtaat gaaggggaaa tttgaaggat
     9961 accgggaaaa taataggact agccaaaaat ttattgatta ttaaattaac tcgaactaat
    10021 ttaggagggg taaatttgaa acctgattat gttgataatc tcgaggaaat ggaaaagttc
    10081 tttgatgaaa aagcgaaaga ttgggatgaa tacgcgaaac acgatcctta taatcattat
    10141 tttaaagcta tttcttctca tgtaagtgaa acaaaagaaa agattagaat attagaactt
    10201 ggttgcggta ccgggttaac attacactcc attttagata aagccccaaa tgcaataata
    10261 acttgtattg atttatcaga gaatatgctg gaggtgctga gtaaaaaatt ttccgagtat
    10321 agctcacaaa ttcaaataag acatggatcg ttttttgaat tcccatttgg taaagaacaa
    10381 tttaactata taatttcttc tcaaacattt caccacttta cacctaacac aaaaaaagca
    10441 ttatatgaaa aaatctactt atctttagaa aggggcggaa agtatattga aggggacttt
    10501 ttagtatcaa tagaagagga gaaagagtca cttaacgagt actataagta tgtagcacct
    10561 cttgaagaaa aggaagaggg aaaatatcat ttcaacatac cgttagcctt agagactcaa
    10621 aagcaattac tatcggattc cggatttaaa tgggaaaacg atatatatta tgaagacgaa
    10681 actgaattag ctgtatatac gggattaaag gaatagtata aatgtcatag acataaaata
    10741 aagaggtgaa atttatgtgt gggatttttg gttttttaaa taactttcat ggtgataaac
    10801 aagatctaat tacaaaacag cataatctta ttaagcatcg aggtccggac ga
//
