LOCUS       NZ_CP024199            10948 bp    DNA     circular CON 03-MAY-2024
DEFINITION  Thalassospira marina strain CSC3H3 chromosome, complete genome.
ACCESSION   NZ_CP024199
VERSION     NZ_CP024199.1
KEYWORDS    RefSeq.
SOURCE      Thalassospira marina
  ORGANISM  Thalassospira marina
            Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodospirillales;
            Thalassospiraceae; Thalassospira.
REFERENCE   1  (bases 1 to 4530245)
  AUTHORS   Dong,C., Liu,R. and Shao,Z.
  TITLE     Biodiversity and function of Thalassospira species in the
            particle-attached aromatic-hydrocarbon-degrading consortia from the
            surface seawater of the China South Sea
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4530245)
  AUTHORS   Dong,C., Liu,R. and Shao,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2017) State Oceanic Administration, The Third
            Institute, Room 329, Daxue Road 184, Xiamen, Fujian 361005, P.R.
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT Analysis v. 2.3
            Genome Coverage                   :: 300
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002844375.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 04:06:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,760
            CDSs (total)                      :: 4,670
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:03:19
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2771782
            Orig. end                         :: 2782730
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP024199.1.
            Bacteria and source DNA available from Dr. Zongze Shao; The Third
            Institute of State Oceanic Administration, Daxue Road 184#, Xiamen,
            Fujian, 3610015, China, Tel: 86-592-2195321.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10948
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="5"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10948
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10948
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5948](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5948)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(1230..1243)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: medium, score: 21.93"
                     /tool="antismash"
     misc_feature    complement(1288..1302)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 19.51"
                     /tool="antismash"
     misc_feature    complement(1415..1428)
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: strong, score:
                     18.25"
                     /tool="antismash"
     gene            complement(1496..2449)
                     /locus_tag="CSC3H3_RS12635"
                     /old_locus_tag="CSC3H3_12630"
     CDS             complement(1496..2449)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1205: ABC
                     transporter, carbohydrate uptake"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092502.1"
                     /locus_tag="CSC3H3_RS12635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CSC3H3_12630"
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="WP_101285044.1"
                     /transl_table=11
                     /translation="MKGLKKLAIAGATMCIGMGLSLAAQAEMIIGFSQIGSESGWRTSE
                     TASIKAEAEKRGIDLKFSDAQQKQENQIKAVRSFIAQGVDGILLAPVVETGWDQVLKEA
                     KDAGIPVVLVDRGVAADKSLYLTKVASDFVQEGALAASWLAAKTNGVCDLVELQGTVGS
                     SAANDRKAGFQSIVDNFPNMKILRSQSGDFTRSGGKEVMESFLKAENGGKNICAVYAHN
                     DDMALGAVQAIKEAGLEPGKDILIVSIDGVPDIFKAMADGDTNASIELNPHLGAPAFDA
                     IKASMDGKDVPKWIKANGPLYLPDTAAEEYKKRSGS"
     misc_feature    2562..2575
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 19.81"
                     /tool="antismash"
     misc_feature    complement(2562..2576)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 19.87"
                     /tool="antismash"
     gene            2599..3606
                     /locus_tag="CSC3H3_RS12640"
                     /old_locus_tag="CSC3H3_12635"
     CDS             2599..3606
                     /EC_number="5.1.3.-"
                     /GO_function="GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA]"
                     /GO_function="GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /locus_tag="CSC3H3_RS12640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CSC3H3_12635"
                     /product="aldose epimerase family protein"
                     /protein_id="WP_101285045.1"
                     /transl_table=11
                     /translation="MTAQHAAQTITTLENQKIRARFCNQGARIMYLGLKNEGNDPVNIA
                     LGFSYIGDYARQDSYIGATCGRYANRIAKARYDNHHQNTVQLAANEGENLLHGGPEGFD
                     RRIWMVMEETASSVVYALSSPDGDQGFPGNLHCTVRYELAGNSLRCEISATCDAPTIVN
                     MTNHSYWNLSGRFDGSAADHELQIHADQYLPVDNGLIPLPGRAEVTGTTFDFRTPRAIA
                     SPEELQAGKIIEYDHNFCLSGRRGTLRKIASLFHAPTNRRLTLSSTEAGLQFYPARHFT
                     PDMTSQDGKSLHPQCAIALEPQTFPDSPNRDDFPSPFLNPGDTYSHVMVWQIEG"
     gene            complement(3581..4153)
                     /locus_tag="CSC3H3_RS12645"
                     /old_locus_tag="CSC3H3_12640"
     CDS             complement(3581..4153)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092468.1"
                     /locus_tag="CSC3H3_RS12645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CSC3H3_12640"
                     /product="DoxX family protein"
                     /protein_id="WP_101285046.1"
                     /transl_table=11
                     /translation="MQITARLSTQITRWGDIFRSHWDVWLGGLGARLVFAAVLWPYYLN
                     SAMTKPGMGPLGIFMPGPGAFAQIIPAVAEAHAYNPAAIAFFPWHLIVIAGTLAEFTLP
                     ILIVLGVFTRLSALGMIGFIIVQSLVDIYAHGAMSGALFDGDPTSVLDQRLLWIFPLVV
                     LLATGGGRFAIDRLLKQFVSNLRSAKP"
     gene            complement(4156..5004)
                     /locus_tag="CSC3H3_RS12650"
                     /old_locus_tag="CSC3H3_12645"
     CDS             complement(4156..5004)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /locus_tag="CSC3H3_RS12650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CSC3H3_12645"
                     /product="DNA-binding domain-containing protein"
                     /protein_id="WP_101285047.1"
                     /transl_table=11
                     /translation="MSNQNAARAGVLSHATTTMPDAEGDFHHAFSLALMDHDPTAHPAG
                     LNDRAARRFAIYRNNVHRGLQEALAAAYPVIKSLVGDGFFYGLAGEYMRTENHRLPSLA
                     LYGAGFPGFLAEHAVAAKLPYLPDIARLERARLEVLHAADAPAINAASLVGYEDRLAML
                     CFAVHPAVKLVVSQFPIHAIWQANQPERQQGGAVHNSGPVQIANRAEAVLISRPHYTLK
                     QACLGGAGVEFARQLLAGQSVGAAYETALAQDANFDVTPVFAQLLASGMFGAFTLGEDG
                     L"
     gene            complement(5001..5948)
                     /locus_tag="CSC3H3_RS12655"
                     /old_locus_tag="CSC3H3_12650"
     CDS             complement(5001..5948)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891959.1"
                     /locus_tag="CSC3H3_RS12655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CSC3H3_12650"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_101285048.1"
                     /sec_met_domain="DUF692 (E-value: 5.7e-89, bitscore: 295.8,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAPQTNPNHQLSPNHPQNRIFKNVPPSAGLPRRAGVGFKAEHAS
                     DVFENADPVSWFEVHPENYMVAGGPRLKMLSQLREKFDISLHGVGLSLGGWEPLDKAHL
                     AALRGLVDRFEPAQVSEHIAWSGHDGKYLADLLPTPLTTASLRHLVDAIDQVQNAIGQR
                     ILIENPTSYLALPQNSMPETDFIIQAARQSGCGLLIDVNNIFISAHNLGFDGRDYVDVL
                     PADLIGEIHLAGHEQDADTSDNVLIDTHSRPVADPVWALYRRLVERIGATPTLIEWDND
                     VPDWQTLAREAKQANAILDGLGDRADILAQKVAS"
     gene            complement(6020..6316)
                     /locus_tag="CSC3H3_RS12660"
                     /old_locus_tag="CSC3H3_12655"
     CDS             complement(6020..6316)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092465.1"
                     /locus_tag="CSC3H3_RS12660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CSC3H3_12655"
                     /product="DUF2282 domain-containing protein"
                     /protein_id="WP_101264637.1"
                     /transl_table=11
                     /translation="MKNALVLAAALSAGLTMAAADASAAGDQEKCFGVSMAGQNECAAG
                     PGTTCAGTSKVDYQGNSWKLVPAGTCEEMTFTTADGRMVHGSLTKLDRDLPEM"
     gene            6872..7918
                     /locus_tag="CSC3H3_RS12665"
                     /old_locus_tag="CSC3H3_12660"
     CDS             6872..7918
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1039: aldo/keto reductase family oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /locus_tag="CSC3H3_RS12665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CSC3H3_12660"
                     /product="aldo/keto reductase"
                     /protein_id="WP_101285049.1"
                     /transl_table=11
                     /translation="MRYHLLGKHTGLRISELALGTGAFGKGWGYGTDLEESGRILGRYL
                     DAGGNFIDTADSYQFGDSEEWLGKLITSNRNDIVLASKYTMGASAQGTLAGSGNSRKNM
                     VMSLEASLKRLNTDHLDIFWVHFSDNMTPGEEILRGFEDLARAGKILYAGLSDFPAWRV
                     SRMATLAELRNTLPIAAIQVEHSLVQRSTEHELLPMADALGLATLAWSPLGGGMLTGKY
                     RNGEKGRAEGLGGRVFQPENSAQRTAILDTVLAIASEKGATPGQVAIAWVAAKGSLPII
                     GPRTLAQLDDNLGAAELVLSIDEIARLDDASAIAPIFPHSMINSEPLRNLATGGKLSQV
                     HMPTQTVA"
     gene            7978..9285
                     /locus_tag="CSC3H3_RS12670"
                     /old_locus_tag="CSC3H3_12665"
     CDS             7978..9285
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1137: Major
                     facilitator superfamily MFS 1"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433919.1"
                     /locus_tag="CSC3H3_RS12670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CSC3H3_12665"
                     /product="MFS transporter"
                     /protein_id="WP_101285050.1"
                     /transl_table=11
                     /translation="MLAHFLSHRLDRRNIHYGWVMAAVTFLTMLGTAGAVGAPGVFIVP
                     LEKQFGWDAAEISSALAIRFALFGMIGPFAAAFMNRFGVRKVVMASLLIVLCGLLSSLA
                     MTQLWQLVLLWGVVVGLGTGLTAIVLGATVATRWFHARRGLVIGLLTASSATGQLVFLP
                     ILARVTDQYGWQAALALICSFLAFVAFCVWLLMHNHPADVNLPAYGADDVTPLPSRNSS
                     LGQLMLSPITILREAARTRAFWILFISFFICGASTNGLIQTHFIALCGDYSIAAVAAAS
                     MLAMMGIFDFFGTIGSGWLSDRFDSRKLLFTYYGLRGLSLVYLPFSDFTLYSLSIFAIF
                     YGLDWIATVPPTVKLVAQNFGGIKTNVVFGWIFAGHQLGAASAAFGGGLARTTYNSYLP
                     AFFTAGILCIAAALMILLMKEAASETTPPKQATANS"
     gene            9521..10126
                     /locus_tag="CSC3H3_RS12675"
                     /old_locus_tag="CSC3H3_12670"
     CDS             9521..10126
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CSC3H3_RS12675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="CSC3H3_12670"
                     /product="hypothetical protein"
                     /protein_id="WP_215907498.1"
                     /transl_table=11
                     /translation="MKKIVLPAVLLLTTPCSIHPLWAAEDLTPASAFQNIPDDHQRSLK
                     LFEEMAKVLTSPRCLNCHPVKGGPTQGDDMHPHNPPMVRGDGDFGPPGMHCSTCHGSQN
                     VDFAGISGSIPGHEPWQLAPVSMGWQGLSIGEICRSIKDPNHNGDRTLEELITHHSEDG
                     LVGWAWHPGAGRTPAPGTQVQFGELTRAWVETGAACPD"
     misc_feature    complement(9522..9536)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 19.96"
                     /tool="antismash"
     gene            10174..10656
                     /locus_tag="CSC3H3_RS12680"
                     /old_locus_tag="CSC3H3_12675"
     CDS             10174..10656
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /GO_function="GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942855.1"
                     /locus_tag="CSC3H3_RS12680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CSC3H3_12675"
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="WP_101286227.1"
                     /transl_table=11
                     /translation="MYELNINKQTYQIEADADTPLLWVLRDILGLTDTKFGCGAGLCGA
                     CTVHIDGMAQRACLTPISEVTDAEILTLAGILETETGKKLQQAWNDLDVVQCGYCQPGQ
                     IMSAAALLEETGKPSDQDISNSMAGNICRCGTYDRIRQAIHHAASIELPHTMEPAK"
ORIGIN
        1 cggaggatgc caccagcaaa gccagcccct cgtcacacag atcccggatc aacttgatga
       61 tatcggcatg ggcgccgaca tcgatgccac gtgtcggttc atccaaaatc agaaaaaccg
      121 gttttgaaac cagccagcgg gccagaataa ccttttgctg gttaccaccc gaaagctggc
      181 caacgggttt ttcccggtcc ggggtggcga taccaagggc cgcgatcatg tcatcggcga
      241 atttatcctg atcggcgcgg ggaatggggc gtagccagcc acgccgcccc tgcagggcca
      301 gggcgatatt ttcgcgaaca ccaagttcgg gcacgatgcc atcctgtttg cggtcttccg
      361 ggcacaggcc gaaccctgca cgaatggcct ggcgcgggga ccttaacaaa accggtttgt
      421 catcaagaaa taccagcccg ctatcggcgc gatgcgcacc aaataccagt tcggcaagtt
      481 ccgtccgccc tgatcccagc aaaccggcaa ggccgacaat ttcgccacca gaaagttcca
      541 gtgaaacagg tttcaacacc cgcttcttgc ccagattttt aatttgaatg cgattgccgc
      601 gatccttggg cgtgtccgac gcatgatggc ggtgcgaact gatttccgcc agttcccggc
      661 ccagcatatg attgatcaga tcgatttggg ggagttgcgc ggtttcaaag gtgccaacaa
      721 ggcggccatt gcgcaaaacc gtgatgcggt ctgtgacctc atagacctga tcgagaaaat
      781 gggtgacaaa aacaatgccc aggcccttgg ccttaagcgc gcgcataatg ccaaacagcg
      841 tcctgatctc gtccgcatcc agcgacgcgg tgggttcatc caggatcaga acccgcgcat
      901 caagggcaac ggcacgcgca attgccacca attgctggat cgccaccgaa taggaaccaa
      961 gtgcgcggtt gatatcaata tccagcccca gtgtttttag cgcctggcgc gcatcttcag
     1021 cagttttttt ccatgaaatc aggccaaact tgcgggtctg gcgttccagc atcaggtttt
     1081 cggcgaccga aagggtcggc accagattga cttcctgaaa aaccgtggaa atgcccagtt
     1141 gctgcgcctc gccgggatgg cccggatgaa tttcgcgccc atcaagggta acggtgccag
     1201 catcgcgcgg atgcaccccg gttatcacct tgatcagggt cgacttgccc gcgccatttt
     1261 ccccgagaag agcgtgaatt tcgccggaat ttagcgaaaa atcaacgtcc tgcagggctt
     1321 tgacgccggg aaagaacttc cccacaccgc gcgcagacag cacggtttgt gcccgatctg
     1381 ccatatgatc ctccctgatc tgtcaggctg ttgcgcaaaa ggcgtgcccc cgtttttcaa
     1441 aggacacgcc attacgccag ccatcaggcc gggtgttttt cacccggcac gattgtcagc
     1501 taccgctgcg ttttttgtat tcttcggccg ccgtatcagg caggtaaagc ggaccattcg
     1561 ccttgatcca tttcggcaca tccttgccat ccatcgaagc cttgatggca tcaaatgccg
     1621 gtgcacccag atgcgggttc agttcgatcg aggcattggt gtcgccatcg gccatggctt
     1681 tgaaaatgtc gggaacacca tcgatcgata cgatcaggat atccttgccc ggttccagcc
     1741 cggcttcctt gatcgcctgg acagcaccca gcgccatatc gtcattatgg gcgtaaacgg
     1801 cacaaatgtt tttgccgcca ttttccgcct tcaggaagct ttccatcact tccttgccac
     1861 ccgaacgggt gaagtcaccc gattgcgagc gcaggatttt catgttgggg aaattatcga
     1921 caatcgactg aaaacccgcc ttgcggtcat tggcagccga tgacccgacc gtaccctgca
     1981 attcaaccag gtcacaaacg ccattggttt tcgctgccag ccaggatgcg gccagcgcac
     2041 cttcctgcac gaaatcagat gccaccttgg tcagatacag cgatttatcg gcagcaaccc
     2101 cacggtcaac cagcaccacg gggataccgg catccttggc ttctttcaaa acctggtccc
     2161 aaccggtttc aacaaccggt gccagcaaaa tgccatccac gccctgcgca ataaaggaac
     2221 gcaccgcctt gatctggttt tcctgttttt gctgcgcatc ggaaaatttc agatcaatcc
     2281 cacgcttttc ggcctcggcc ttgatcgatg cggtttccga tgtccgccag ccgctttccg
     2341 acccgatctg ggaaaacccg ataatcattt ctgcctgtgc ggcaagcgac aatcccatac
     2401 cgatgcacat ggttgcaccg gcaatagcca gctttttaag ccctttcatt atttcctccc
     2461 tgaattggcc ttctcgacaa aagccatata gtattacaat atgattgtta ccaagacaac
     2521 gacatcagca accaccaatt gaaaagcgca gttttctgcg aatttttcag gaaaaagaaa
     2581 cgtcagggaa cagccggaat gacagcgcaa cacgcggccc aaaccatcac aaccctagaa
     2641 aaccaaaaga ttcgcgcacg gttttgcaat cagggcgcgc gaatcatgta tttaggcctg
     2701 aaaaatgaag gtaatgatcc cgtcaatatt gcattgggat tttcctatat cggcgactat
     2761 gcccgacagg acagctatat cggcgcaacc tgcgggcgat atgccaaccg cattgccaag
     2821 gcgcgctatg acaaccatca tcaaaacact gtgcagctgg ctgccaatga aggcgaaaac
     2881 ctgctgcatg gcgggccgga agggtttgat cgccggatat ggatggtgat ggaagaaacc
     2941 gccagcagcg tggtttatgc cctgtcatcg cctgatggcg accagggttt tcccggtaac
     3001 ctgcattgca cggtacgata tgaacttgct ggcaacagcc tgcgatgtga aatttcggcc
     3061 acctgcgatg cgccgaccat cgtgaatatg accaatcaca gttactggaa tctttcgggt
     3121 cggtttgatg gcagtgccgc cgatcacgaa ttgcaaatcc atgccgacca atacctgccc
     3181 gtcgataatg gccttattcc cctgcccggc cgggcagagg taaccggcac aacatttgat
     3241 tttcgcaccc cgcgcgccat tgcctcgccc gaggaattac aggcgggtaa aatcatcgaa
     3301 tatgaccata atttctgcct gagcgggcgg cgcggtactt tgcgcaaaat tgccagcctg
     3361 tttcatgccc ccaccaaccg tcggctgacc ctttcaagca ccgaggcagg gttacagttt
     3421 tacccggcgc gccattttac gcccgatatg acatcgcagg atggcaaatc gctgcatccg
     3481 caatgcgcca tcgcgctgga gccacaaaca tttccggaca gccccaatcg cgatgatttt
     3541 ccatcaccgt ttttaaatcc cggcgatacc tattcgcatg tcatggtttg gcagatcgaa
     3601 ggttagaaac aaactgcttt aacaggcggt caatggcaaa acgccccccg cctgttgcca
     3661 gcaacaccac cagcgggaaa atccacaata accgctggtc cagcacactg gtcgggtcgc
     3721 catcaaaaag cgcgcccgac atcgccccat gggcataaat atcgaccagc gactgaacaa
     3781 taataaaccc gatcatgccc agtgccgaaa ggcgggtaaa cacacccagc acaatcagga
     3841 ttggcagggt aaattcggcc aatgttcctg caatcacaat caaatgccag gggaaaaagg
     3901 caatcgccgc cgggttatag gcgtgtgctt cggccaccgc cggaatgatc tgggcaaagg
     3961 cccccggccc tggcatgaag atgcccaatg gccccatgcc aggtttggtc atggcgctgt
     4021 tcagataata gggccacaag accgcagcaa aaaccagacg cgcgcccaac ccgcccaacc
     4081 atacatccca atggctgcga aatatatcgc cccatcgggt gatttgggtg ctcagccttg
     4141 ccgttatctg catgttcaaa gcccgtcttc ccccagggta aatgcaccaa acatccccga
     4201 tgccagcaat tgggcaaaaa ccggggtgac atcaaaattg gcatcctgtg ccagtgcggt
     4261 ttcataggct gcaccaacgc tttgccctgc cagcaattgg cgggcaaatt cgaccccggc
     4321 gcctccaagg caggcctgtt ttagggtgta gtgcgggcgg gagatcaaaa ccgcttcggc
     4381 ccggttggca atttgcaccg gcccgctgtt atgtaccgcc ccgccctgct ggcgttccgg
     4441 ctggttggcc tgccagatgg catgaatggg aaactgcgat accaccaatt tcactgccgg
     4501 gtgaacggca aagcacaaca ttgccagcct gtcttcatac ccgacaaggg acgcggcatt
     4561 tatcgccggg gcatccgccg catgaagcac ttccagccgg gcgcgttcaa gacgggcgat
     4621 atcgggcaga tagggcaatt ttgccgccac cgcatgttcc gccaggaaac cgggaaagcc
     4681 cgcaccataa agcgcaagcg atggcaggcg gtgattttcg gtgcgcatat attcgcccgc
     4741 cagcccgtaa aaaaacccat cgcccaccag tgattttatt accggatagg ctgctgccaa
     4801 ggcttcctgc aggccacgat gaacattgtt gcgataaatg gcaaaccggc gcgcagccct
     4861 gtcattcaac ccggcgggat gcgccgttgg gtcgtgatcc atcagggcaa ggctaaacgc
     4921 atgatgaaag tcgccttcag catcaggcat ggtggtggtg gcatgcgata aaacaccggc
     4981 gcgtgccgcg ttctgattgc tcatgacgcc accttttgcg ccaaaatatc ggccctgtca
     5041 ccaaggccat ccagaatggc gttggcctgc ttcgcctcgc gggcaagggt ttgccaatcg
     5101 ggcacgtcat tgtcccattc gatcagggtt ggggtggcac caatacgttc caccagccgc
     5161 cgatacagcg cccaaaccgg gtctgccacc gggcgggaat gggtatcaat cagcacgtta
     5221 tcactggtat cggcatcctg ttcatgcccg gccaggtgga tttcgccgat caaatcggca
     5281 ggcagcacat cgacataatc ccggccatca aaacccagat tatgggcact gatgaaaatg
     5341 ttgttcacat caatcagcaa cccgcagccc gactggcgtg ccgcctgaat gataaagtcg
     5401 gtttcgggca tcgaattttg cggcagtgcc agatacgaag taggattttc gatcaggatg
     5461 cgctggccaa tggcgttttg cacctggtca atcgcatcga caagatggcg caacgacgct
     5521 gttgttaatg gtgtcggcag cagatcggcc aggtatttac catcatggcc cgaccaggcg
     5581 atatgttcgg aaacctgtgc cggttcaaac cggtcaacaa gtccgcgcaa ggcggcaagg
     5641 tgggctttgt ccaacggttc ccacccgcca agcgacaggc caaccccatg cagggaaatg
     5701 tcgaatttct cgcgcaattg cgacagcatt ttaaggcgcg ggccacccgc caccatgtag
     5761 ttttccgggt gaacttcaaa ccacgatacc ggatcggcat tttcaaaaac gtcgctggca
     5821 tgttcggcct taaagccaac accggcgcgc cgtggcaaac cggccgatgg cgggacattt
     5881 ttgaaaatac ggttctgcgg atggtttggc gaaagctggt ggttcgggtt tgtctgtggt
     5941 gccgtcatga tatgactccc ctgcccatga taaaagaaaa gcccggtgcg ccagatcgct
     6001 ggcacaccgg gaaaatggct tacatttccg gcagatcgcg gtcgagcttg gtcaggctgc
     6061 catgaaccat gcggccatcg gcagtggtga aggtcatttc ttcacaggtg ccagccggaa
     6121 ccagcttcca ggaattaccc tgataatcga ccttggaagt accggcacag gtcgtgcccg
     6181 gaccagcggc gcattcgttc tggccagcca tggaaacacc aaagcatttt tcctggtcac
     6241 ccgctgccga tgcgtcagcg gcggccatcg tcaatccggc agaaagtgcg gctgccaata
     6301 caagtgcgtt tttcatttgc ttttcccttt ttaaaaattc ccgacgttac atgtcacaag
     6361 cggtctttcc aattcgctgt gacaacccgg tgatagcaac cgcaaggcac cccgtcatcg
     6421 caacaactgc acacagcgca tcaacacggt gtgaggataa atccgtttaa attcacacca
     6481 ttgtgagaga aaccagcacc aaacaagcca gcctgttttc cagtcacgat aaaaacccag
     6541 cccgattcac gaaacgcaac atcaacccaa ggttacgaat ggtgcattac cagcacgcat
     6601 ggctgcatcg cctttttgcc gggcatgtcg tggttcggca gttttggaaa agcgcggaaa
     6661 atattggaat aaccgtttta aaaccatcac ttccggccgg ctgcatggtc ggttttgggc
     6721 atgctttttt ccggttcatt tccctttctt aattcgcgtc aaacgcgcca gcgcaaattc
     6781 cgcttgaccg ttaaattaca atcatcattt aatcacgtta aattatgaac atcatttatt
     6841 ggcatttaaa cgccatctga aaggatcacg aatgcgttat cacctgttag gcaaacatac
     6901 cggtttgcgg atttcggaac tggcattggg cactggtgca tttggcaaag gctggggata
     6961 tggcaccgat ctggaagaat caggccggat tttgggccga tatctggatg ccgggggcaa
     7021 ttttatcgat acggccgact cttatcagtt tggtgattcg gaagaatggc tggggaaact
     7081 gatcacatcg aaccgcaatg acatcgtgct ggccagcaaa tacacgatgg gcgcaagtgc
     7141 ccagggcaca ttggccggta gcggcaatag ccgcaaaaac atggtgatgt cgctggaagc
     7201 cagcctgaag cgccttaata ccgaccatct tgatattttc tgggttcatt tcagcgataa
     7261 tatgacaccg ggcgaagaaa ttctgcgcgg gttcgaggac ctcgcccggg ccggtaaaat
     7321 tctttatgcc gggctttcgg attttccggc atggcgggta tcgcgcatgg cgacccttgc
     7381 cgaactgcgc aacacattac ccattgccgc cattcaggtt gaacacagcc tggttcaacg
     7441 tagcaccgaa catgaattgc tgcccatggc cgatgccctt ggtttggcaa cgctggcatg
     7501 gtccccgctg ggcggtggca tgttaacggg caaatatcgc aatggtgaaa aaggccgggc
     7561 cgaaggcctt ggcgggcgtg tatttcagcc tgaaaacagt gcgcagcgca ccgcaatcct
     7621 tgatacggta ttggcaattg ccagtgaaaa aggtgcaaca ccgggccagg ttgccattgc
     7681 ctgggttgcc gccaaaggct cccttcccat tatcggtccg cgcacgctgg cccagcttga
     7741 cgataatctg ggggcggctg aactggtgct aagcatcgat gaaattgccc gcctggatga
     7801 cgcaagcgcg attgccccga ttttcccgca cagcatgatc aatagtgaac cgctgcgtaa
     7861 tcttgcgacc ggcggaaaac tgtcacaggt gcacatgccg acccaaaccg ttgcctgacc
     7921 acccctttcc ctttaccggg ccgggccacc ttgatacccg gccagcagga atttgacatg
     7981 cttgcacatt ttttatcgca ccgtctggac cggcggaaca tccattatgg ctgggtcatg
     8041 gccgccgtaa cgtttttgac catgctgggc accgccgggg ccgtgggcgc gccgggggtg
     8101 tttatcgtcc cgcttgaaaa acagtttggc tgggatgctg ccgaaatttc atcggcactt
     8161 gccattcgtt ttgccctgtt tggcatgatc ggcccctttg cagctgcctt catgaaccgc
     8221 tttggtgtgc gcaaggtagt gatggcatcg ctgctgattg tgctgtgtgg cctgttatcg
     8281 tcactcgcga tgacgcaact ttggcaattg gtgctgttat ggggtgttgt tgtgggcctt
     8341 ggcaccgggc ttacggccat tgttttgggg gcaacggttg ccacgcgctg gttccatgcc
     8401 cggcgcgggc tggtgattgg gttgcttacg gccagttcag ccactggcca attggtgttt
     8461 ctgcccattc ttgcccgtgt gacagaccag tatggctggc aggcggcatt ggcgctgatc
     8521 tgttcattcc tggcatttgt tgccttttgt gtctggcttt tgatgcataa tcacccggca
     8581 gatgtgaacc tgcctgctta cggggcggat gacgtaacac cactgcccag ccgaaacagc
     8641 agtctgggcc aattgatgct ttcgcccatc accatcctgc gcgaggcagc acgaacacgt
     8701 gccttctgga ttttgttcat cagctttttt atctgtggtg ccagcaccaa cggcctgatc
     8761 cagacccatt ttatcgcact ttgcggcgat tacagcattg ccgccgtggc cgctgccagc
     8821 atgctcgcca tgatggggat tttcgatttt ttcggcacca tcgggtcggg ctggctatca
     8881 gaccggtttg acagccggaa acttctgttc acctattatg gcctgcgtgg gctgtcgctg
     8941 gtttacctgc cgttttcgga cttcacgctt tatagcctgt cgatctttgc aattttttat
     9001 ggccttgact ggattgccac cgtgccaccc acggtaaagc tggtggcgca aaattttggc
     9061 ggcatcaaaa ccaatgtggt tttcggctgg atctttgccg gccaccagtt aggtgcggcc
     9121 agtgcggcct ttggcggtgg tttggcccgc acaacatata acagctacct gcccgccttt
     9181 ttcaccgccg gaatcctgtg cattgcagcg gcccttatga tcctgctgat gaaagaagcc
     9241 gccagcgaaa ccacgccccc aaaacaggca accgcaaaca gctaaaaccc ggcacctgac
     9301 gaaaaccggc acaacctgac aaaggcccga tcatcactga tcgggccttt gtgcgtttca
     9361 gggtacgccc atgcaccctg cctgttcagc aaaattgaac actgtcttta attatcacca
     9421 ccttctgcca accgatcttt tttcaaatac tgatcaaaac gcgcaacgcc cctgttgagg
     9481 ctttctgcag cgcaacactg ccagaaccga gatcagattt atgaaaaaga ttgtcctgcc
     9541 tgcggtcctg cttttgacaa cgccatgttc tattcacccg ctttgggcgg cagaagattt
     9601 aacgccagca tctgcctttc aaaacatccc ggatgaccat caacgaagcc tgaaattgtt
     9661 cgaagaaatg gcaaaggtgc tgaccagccc gcgctgcctg aattgccatc ccgtaaaagg
     9721 cgggccaacg cagggcgacg atatgcatcc gcataatcca cccatggtgc gcggtgatgg
     9781 cgattttggc ccacctggca tgcattgttc aacatgccat ggcagccaga atgtcgattt
     9841 cgccggaata tcaggctcca tccccgggca tgaaccctgg cagctcgccc cggtatcaat
     9901 gggctggcag ggattatcca tcggcgaaat ctgccgatct atcaaagacc ccaaccataa
     9961 tggtgatcgc accctggaag aattgatcac ccatcattcg gaagacggac tggttggatg
    10021 ggcttggcat ccgggggccg ggcgcacacc ggcacccggc acgcaggtgc aatttggcga
    10081 actgacacgt gcgtgggtcg aaactggtgc tgcatgcccg gactgatcgc cacctctccc
    10141 cacttgcatc cacacgggaa tacgaacgcc atgatttacg agcttaacat taataagcag
    10201 acctatcaga tagaggccga cgcagacacg ccgcttttat gggtgttacg cgatatcctt
    10261 ggcctgacag atacaaaatt tggctgcggc gcaggtttgt gcggtgcctg caccgttcat
    10321 attgatggca tggcgcagcg ggcctgcctt accccgatat cagaggtaac agatgccgaa
    10381 attttgaccc ttgcgggtat tttggaaacc gaaacaggca agaagctcca gcaagcatgg
    10441 aacgaccttg atgtcgtcca atgcgggtat tgccagccag gtcaaatcat gtcggcggcc
    10501 gcacttctag aagaaacggg caaaccctct gatcaggata tcagcaattc gatggcagga
    10561 aatatatgca gatgcggcac ttatgaccgt attcgccagg ccattcatca tgccgcaagc
    10621 atagagctgc cacacacgat ggaacccgca aaatgacaaa gccccaaaca tcacctgttc
    10681 gtatttcacg acgcttgttt ctgtttggcg gcgccgctgt gggggcagga attgcgattg
    10741 gcatgaatat cccgcttgcg atggcacagg ctgagacctc caaaaccggg tcgtttgagc
    10801 ccaatgcgtt tcttcgtata gaggcagacg gcgatatcat cattgtgatg ccctatgttg
    10861 aaatgggcca gggcatttat acgaccgttg cgatgctgat cgccgaggaa cttgatatcg
    10921 gcctggaaca catcagggtt gaacatgc
//
