LOCUS       NZ_CP024199            10402 bp    DNA     circular CON 03-MAY-2024
DEFINITION  Thalassospira marina strain CSC3H3 chromosome, complete genome.
ACCESSION   NZ_CP024199
VERSION     NZ_CP024199.1
KEYWORDS    RefSeq.
SOURCE      Thalassospira marina
  ORGANISM  Thalassospira marina
            Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodospirillales;
            Thalassospiraceae; Thalassospira.
REFERENCE   1  (bases 1 to 4530245)
  AUTHORS   Dong,C., Liu,R. and Shao,Z.
  TITLE     Biodiversity and function of Thalassospira species in the
            particle-attached aromatic-hydrocarbon-degrading consortia from the
            surface seawater of the China South Sea
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4530245)
  AUTHORS   Dong,C., Liu,R. and Shao,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2017) State Oceanic Administration, The Third
            Institute, Room 329, Daxue Road 184, Xiamen, Fujian 361005, P.R.
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT Analysis v. 2.3
            Genome Coverage                   :: 300
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002844375.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 04:06:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,760
            CDSs (total)                      :: 4,670
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:03:19
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2096616
            Orig. end                         :: 2107018
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP024199.1.
            Bacteria and source DNA available from Dr. Zongze Shao; The Third
            Institute of State Oceanic Administration, Daxue Road 184#, Xiamen,
            Fujian, 3610015, China, Tel: 86-592-2195321.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10402
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="4"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10402
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10402
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5402](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5402
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(290..306)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: medium, score: 21.64"
                     /tool="antismash"
     gene            complement(2166..2663)
                     /locus_tag="CSC3H3_RS09565"
                     /old_locus_tag="CSC3H3_09560"
     CDS             complement(2166..2663)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1135: MarR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891073.1"
                     /locus_tag="CSC3H3_RS09565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CSC3H3_09560"
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_101269807.1"
                     /transl_table=11
                     /translation="MDKLHDALISIRRILRASDIHAKRLGKLAGLKTSQLLVLQSLDDS
                     GEMTVGEIAAKVNLAQASTTALVDKLQSMGLVQRARGHSDKRKVYVRLTDAAHDVLARA
                     PMALHDRFSEKFNDLEEWEQSFLVAALQRVSTMMDAGDIDVAPLLDHEVIVEKPADGGR
                     NA"
     misc_feature    complement(2849..2863)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 19.79"
                     /tool="antismash"
     gene            2977..3525
                     /gene="ectA"
                     /locus_tag="CSC3H3_RS09570"
                     /old_locus_tag="CSC3H3_09565"
     CDS             2977..3525
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089564.1"
                     /locus_tag="CSC3H3_RS09570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CSC3H3_09565"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_101269805.1"
                     /transl_table=11
                     /translation="MKVATSDGKLNRKANLVIRKPKSTDGAAVNELIADCKPLDENSVY
                     CNLLQCSHFAPTCALAELDGEVVGFVSGYLVPETPERLFIWQVAVSPKARGLKLGKRMI
                     LDILDRPVCHDVTELHTTITTSNAPSQGVFRSVARELETDVNRKVLFEREKHFDGAAAS
                     EILWQIGPFERVDVERVAA"
     misc_feature    complement(3545..3560)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 20.94"
                     /tool="antismash"
     gene            3641..4927
                     /gene="ectB"
                     /locus_tag="CSC3H3_RS09575"
                     /old_locus_tag="CSC3H3_09570"
     CDS             3641..4927
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (22-358). E-value: 2e-80.
                     Score: 262.3. Matches aSDomain:
                     nrpspksdomains_CSC3H3_RS09575_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089563.1"
                     /locus_tag="CSC3H3_RS09575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CSC3H3_09570"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_101284688.1"
                     /sec_met_domain="Aminotran_3 (E-value: 2.1e-76, bitscore:
                     255.1, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVFDRLESEVQSYARSFPVTFKKAEGAWLTDTDGKRYLDFLAGA
                     GSLNYGHNNPILQERLVDYIKANGITHGLDMHSCAKEEFLNAMESKIFKPRNMEYKIQF
                     TGPTGTNAVEAALKLARRVKGRENIISFTNGFHGVTLGSLAITGNEHHRGAAGVSLDNA
                     VSVPFDGYMGDGVDTTDYLDRVLSDNSSGVALPAAIIVETVQGEGGLNAADFDWLKKVE
                     ALCRKHDILLIVDDIQAGCGRTGTFFSFEPAGIKPDMVTLSKSLSGYGLPFAVVLIKPE
                     YDVWHPGEHNGTFRGNNHAFVTATAALEHFWSDDAFAKSVQEKAEFLGKRLEEIATRYG
                     DNGSIYRKGRGLMQGICFESGEIADKVTTECFGRNLIIETSGPEDEVVKCLVPLIISME
                     DLAYGLDILEVSTAKIMGKNVVTAKAAAE"
     aSDomain        3707..4714
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CSC3H3_RS09575_Aminotran_3.1"
                     /evalue="2.00E-80"
                     /label="CSC3H3_RS09575_Aminotran_3.1"
                     /locus_tag="CSC3H3_RS09575"
                     /protein_end="358"
                     /protein_start="22"
                     /score="262.3"
                     /tool="antismash"
                     /translation="KKAEGAWLTDTDGKRYLDFLAGAGSLNYGHNNPILQERLVDYIKA
                     NGITHGLDMHSCAKEEFLNAMESKIFKPRNMEYKIQFTGPTGTNAVEAALKLARRVKGR
                     ENIISFTNGFHGVTLGSLAITGNEHHRGAAGVSLDNAVSVPFDGYMGDGVDTTDYLDRV
                     LSDNSSGVALPAAIIVETVQGEGGLNAADFDWLKKVEALCRKHDILLIVDDIQAGCGRT
                     GTFFSFEPAGIKPDMVTLSKSLSGYGLPFAVVLIKPEYDVWHPGEHNGTFRGNNHAFVT
                     ATAALEHFWSDDAFAKSVQEKAEFLGKRLEEIATRYGDNGSIYRKGRGLMQGICF"
     gene            5001..5402
                     /locus_tag="CSC3H3_RS09580"
                     /old_locus_tag="CSC3H3_09575"
     CDS             5001..5402
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891076.1"
                     /locus_tag="CSC3H3_RS09580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CSC3H3_09575"
                     /product="ectoine synthase"
                     /protein_id="WP_101269801.1"
                     /sec_met_domain="ectoine_synt (E-value: 6.8e-47, bitscore:
                     156.5, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRTLKECEESGRRVVADNWESTRLSLADDGMGFSFHITTIYAG
                     TETEICYANHFETVYCISGNGEVETIEDGKKYKIVPGTVYILNKHDRHYLRGGTEDMVV
                     ACAFNPPLNGRETHDENGVYPLDGEALAS"
     misc_feature    complement(5425..5435)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
     gene            5496..7001
                     /locus_tag="CSC3H3_RS09585"
                     /old_locus_tag="CSC3H3_09580"
     CDS             5496..7001
                     /EC_number="2.7.2.4"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891077.1"
                     /locus_tag="CSC3H3_RS09585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CSC3H3_09580"
                     /product="aspartate kinase"
                     /protein_id="WP_101284689.1"
                     /transl_table=11
                     /translation="MVSARKQADAGHSVHKIGGTSMTQVQAVMDNILVGDRDESDLYNR
                     IFVVSAYGGFTDLLLENKKTGEAGVYQLYAGSETDWAWGDALNKVADRMCALNEEIFQD
                     QNDLKLANSFVQERVEGTRSCLMDLHRLCSFGHFKLEKHLLTVREMLSAIGEAHSAHNT
                     MLLLRGKGVNAVFADLSGWREPEASTLDERINSVFENIDVKSQMPIVTGYAQCSEGLMS
                     IYGRGYSEVTFSRIACLTGAHEAIIHKEFHLSSADPRIVGEDAVRPIGRTSYDVADQLS
                     NMGMEAIHPRAAKGLRQNGIPLRIANTFEPEHPGTVIDDDFHPDESRVEIVTGIGTSFE
                     IEVFNQDMVGVPGAEEEVLSVLKRYKVPTVTKNLNANTITHYVSAPLKQIRRCVEEIES
                     RQPEASVKTRKVAIVSVIGANISQPGLFARAVSILDDAGVELVASHSPSRRVDLQFVVA
                     EGDFNKAIIALHRGLVEGKKSATATKTKNSKDDGDNVGELAAA"
     misc_feature    5537..5551
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: weak, score: 20.86"
                     /tool="antismash"
     gene            7166..8008
                     /gene="mazG"
                     /locus_tag="CSC3H3_RS09590"
                     /old_locus_tag="CSC3H3_09585"
     CDS             7166..8008
                     /EC_number="3.6.1.9"
                     /codon_start=1
                     /gene="mazG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631833.1"
                     /locus_tag="CSC3H3_RS09590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CSC3H3_09585"
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="WP_101284690.1"
                     /transl_table=11
                     /translation="MSATSNRPAIDQLLQVMKQLRDPENGCPWDIEQDFSTIAPYTIEE
                     AYEVADAIANNDMVELREELGDLLLQVVFHSQMAEEQKLFSFNDVAQSINDKMIARHPH
                     VFGEIDAKTADHVNQNWEKLKAAERQRKADRKGNETPSALDGVASALPALMRAEKLTKR
                     AARVGFDWPDIEQVFDKLSEEIGELRAELTENPVQDRLEDELGDMLFVMANLARKMQVD
                     PEVALRRANHKFTRRFQFIEKELAGRGKTPGQSSLEEMDALWNAAKVEERKTTTKPQE"
     gene            8078..8815
                     /locus_tag="CSC3H3_RS09595"
                     /old_locus_tag="CSC3H3_09590"
     CDS             8078..8815
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /locus_tag="CSC3H3_RS09595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CSC3H3_09590"
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="WP_101284691.1"
                     /transl_table=11
                     /translation="MTASYVAIVLLGAFAGGFVNGLAGFGTGLVALGIWLHVIDPLYGA
                     PLVAICSVAGQLQTLPAIWHAVNIRRLLPMLVGGVLGIPVGILLLGHMAPDLFRLSLGL
                     FLIAFAIFMMFNRSLPAMRWGSKKTEGMVGFCGGILGGLAGLSGPLPTIWVTLKRWEKD
                     ERRSVFQMFNLTILACVAISHLAIDGITVAFSQLVLIALPGTFVGAFAGIRLYRRLNDH
                     RYNLLVLIMLAISGITLVVSEIG"
     gene            complement(8824..9657)
                     /locus_tag="CSC3H3_RS09600"
                     /old_locus_tag="CSC3H3_09595"
     CDS             complement(8824..9657)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1207: inositol monophosphatase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891080.1"
                     /locus_tag="CSC3H3_RS09600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CSC3H3_09595"
                     /product="inositol monophosphatase"
                     /protein_id="WP_245881358.1"
                     /transl_table=11
                     /translation="MTPDIDKVTTIIREVAAEEIAPRFGQLDAGDIDIKTHAQDLVTIA
                     DTEAERILTQRLTALLPGSVALGEEAAHHDAGLQGRVFADNAPIWIIDPVDGTKNFAHQ
                     RQPFRSMLALYRDGVTIAAWIVDPITGDVTVAEKGSGTFHNGKQVTVRSDVTSAASARG
                     FLISYARDRLVKAHGHHNFFEKLSHYSCCGAEYEDIAQGNYDFCAYRTLKPWDHAPGTL
                     LVSEAGGIARYISIDGVFRDYQANGDGPALICASSAELWDEVNTLLLPVFGKKAS"
ORIGIN
        1 agttctgaca tttgttcgac aattgatttc agttctttca tgcgatgtga cgtgccattc
       61 atcgccgccg aaatttcatt ggcgaccgag ctttgttctt ccgtcgcaac agcaatggat
      121 tgcaccgtat cctgaaccgt gacgacctcg gtcacgatgc cgcgtacggc ctcggctgat
      181 tctgaaattt cttcaagcat cgcattaata cgctggctta tatcactggt tgctttttga
      241 gtttgatttg ccagaacctt gacctcgttt gccacaacgg caaaaccttt gcctgcttca
      301 ccggcacgtg cggcctcgat tgtggcatta agggccagaa ggtttgtctg ttcggcaatg
      361 tcagaaataa gccgcacaac atcgcgaatg gccgcagatg tcgtattaag ctgttcaacc
      421 ttgttggcgg cttcattggc actgctggcg gcagatgtgg atttttcggc aactgcgccg
      481 actttatcgg cgacttcggc aatagcatgg tcaagccggc tggcattttc ggcggccacc
      541 cgcacatcct cgtcggttga ccctaccccg ctgctaaccg tcaagccctg ctgggcggcg
      601 ttttccgatg tctggataac gcttttggcc tgggaagcaa gctgctcagc agtgcggtta
      661 agcttttcac ttaatgcacc aacttcggtt tcaaactggt cagcaacctt tagcaatatc
      721 tggcgttgct ggcgttcaac atcacgtttg aaagcttcct gttcggcctt ataacgttca
      781 ttttcaacag cgtagccgca aaacttggca acacagcgag acaatcgggc gatttcatcg
      841 ctgccagtgc gcgccttgac cttcacatta acctgaccca tggcaagatg atcaagggct
      901 tcgaccattt cctcaaacgg tttggcaaga cgaaatgtgg taaaccacca gccaatggtc
      961 agcgcaataa ccgccagaat ggttatggtt aaagaagcga tgaattcttc gagcacgcga
     1021 ctttgatagg atttgatatc ctgatcaatc agggcggcca tggcggcccc tgcccttaaa
     1081 gccagatcaa tattggaact ggcggctgca aaccagtccg acgatgacat gggataggcg
     1141 ttacccgcaa ccccggcagc catgatcttt tcacgcatgg ggttgaaatc atcggtgtaa
     1201 ccttttagcg ctgttgagac cacagcattg atatcggacg gtaaaccttt acgaagaaaa
     1261 gaaatttctg cctgtgtagc ctcgatcgta ccgtgaaatt cgccaaggtt ggatatctgc
     1321 tcgcctgata attttccgcc accaccaata atacccgcaa aataaccgcg ttcccgaccg
     1381 gcgaattcag cccatttcca aagccggtca cgaagctgta ccgactggat gatttcacgt
     1441 ggcagatccg atagcaggtt caattctgcc tcgttacgca aaacggccga tgccatgatc
     1501 aaatcggtcg tcgccccaaa ccagcgcgca gcaagttcac cattggcggg caaggtattg
     1561 gttgcaaaca ttttatcggc gtcggcgcgc aaatattcga caatgcccat cgccttgtca
     1621 tatcgcgtca ggtcaaggcc gtcaccaggc agcaatgcaa gggcggattg cgcgctgtca
     1681 ttacccaatt tacgtttttc ctggatggct ttaactgcat cagccgtggc cttgctttta
     1741 gccccgataa tgccggccgt catgccgcgt tcaacggccc aggacccggc cgcgcgaata
     1801 aaatcgtctg cggcctggtt aatcgtcgct gcacgttctg tttcctgata gatcgcccag
     1861 cttgtagctg catcccacag agacttggca atcagcccgg cccccaacac accaacaacg
     1921 cacaaaatct ttccacgcag ggataattcc atcacgtgat cccccaaaga tcggcctgaa
     1981 ttaatggtgg ttacaattca ccattaattc aatctttggt gtcttttcgc aattacaata
     2041 ggcaaacatt agatgaggtg aatttttatg caccccccct aacctgctgc ggttgcgcca
     2101 taaatcatac ggaaaatgct gacgcagccc ataacaacgc cacgcgtgat tgaaatacca
     2161 aacggtcagg cgttacgacc gccatcagcc ggtttttcaa caatcacttc atgatcaagc
     2221 aatggtgcaa catcgatatc gccggcatcc atcatggttg aaacacgctg aagggcggca
     2281 acaaggaaac tttgttccca ttcctcaagg tcgttgaact tttcggaaaa ccggtcatgc
     2341 agggccatgg gcgcgcgggc aagaacatcg tgtgccgcat ccgtcagccg gacatagact
     2401 ttgcgcttgt ccgaatggcc gcgtgcccgc tgcaccagcc ccatggactg caatttatcg
     2461 acaagcgccg tggttgatgc ctgggccaga tttaccttgg cggcaatttc acccaccgtc
     2521 atttcaccgg aatcatcaag ggactgcaac accagtaatt gcgatgtttt cagaccggca
     2581 agtttaccca ggcgcttggc gtgaatgtcg ctggcgcgaa ggatgcggcg aattgaaatc
     2641 agggcgtcgt gcaacttgtc catatgcgcg tccgaaaaag acgttgtacc tgttttactt
     2701 atacggccca aggcactgaa acacaacgga atttggcaaa acccatcgga aatacatgag
     2761 aatttatatc gaatactata cttttatgac agtcgaaaat tttgaaattc aatttgttcc
     2821 aggatgcgga ataaatatat ttatcgttaa ttttcaatat attgatcaat aacagcgcag
     2881 aaattcgcag gaggctgttg attaaaatat atcgataatc tatgtttctt ctcattcatc
     2941 tagatcgatt gacgaaacaa ttaggcagat agaacgatga aagttgccac aagcgacggc
     3001 aagttaaacc gcaaagccaa cctggtaatt cgtaaaccaa agtccacaga cggggccgcg
     3061 gtgaacgaat tgattgcaga ttgcaagccg cttgatgaga actctgttta ctgcaacctg
     3121 cttcaatgct cgcattttgc accgacctgc gcccttgctg aactcgatgg tgaagttgtc
     3181 gggtttgttt caggctatct tgtgcctgaa acgccggaac gccttttcat ctggcaggtg
     3241 gctgtatcgc ccaaggcgcg cggcctgaaa cttggcaagc ggatgatcct cgatattctt
     3301 gatcgtccgg tgtgccatga tgtgaccgag cttcacacga cgattactac gtccaatgcc
     3361 ccgtcgcagg gtgtgttccg ctctgttgcg cgcgagctgg aaaccgatgt taaccgcaag
     3421 gttctgtttg aacgtgaaaa gcattttgat ggcgcagcag ccagcgaaat cctctggcag
     3481 atcggcccgt ttgaacgtgt cgatgttgaa cgcgttgccg cctgaatttc accttcaatg
     3541 acctgtgaaa aaacgccgaa attccggaac aaaaccggac cttcggcgtt tgatttgaca
     3601 gacggaattt ttattttctc cccaaatgaa agagagaatt atgactgtat ttgatcgcct
     3661 tgaatcggaa gttcagagct atgcccgctc cttccctgtg accttcaaaa aagcagaagg
     3721 cgcctggctg accgatacgg atggaaaacg ctatctggac tttctggccg gtgccggcag
     3781 cctgaattat ggccataaca acccgatcct gcaggaacgc ctggttgact atatcaaggc
     3841 aaacgggatc acgcatggcc tggacatgca tagctgcgcc aaggaagaat tcctgaacgc
     3901 gatggagagc aaaatcttca agccgcgtaa tatggaatat aaaatccagt ttaccggccc
     3961 gaccggcacc aatgcggttg aagctgccct taagctggcg cgccgtgtga aaggccgtga
     4021 aaacatcatt tccttcacca atggcttcca tggtgtaacc ttgggttcgc ttgcgattac
     4081 cgggaatgaa catcatcgtg gcgcagctgg cgtatcgctt gataatgccg tttccgttcc
     4141 gtttgacggg tatatgggcg atggcgttga taccaccgac tatcttgatc gcgtgctaag
     4201 cgacaattca agtggtgttg ccctacccgc cgccatcatt gtggaaaccg tccagggtga
     4261 aggtggcctg aatgccgccg attttgactg gctgaaaaag gtcgaggccc tgtgccgcaa
     4321 gcatgacatc cttctgattg ttgatgacat tcaggcaggt tgtggtcgta ccggtacgtt
     4381 cttcagcttt gagcctgccg ggatcaagcc ggatatggtg acgctgtcaa aatcgctgtc
     4441 gggttatggc ctgccgtttg ctgttgtgct gatcaagccg gaatatgacg tttggcatcc
     4501 gggtgaacat aacggcacct tccgcggtaa caaccatgcc tttgttacgg caaccgcagc
     4561 gctggaacat ttctggtcag atgatgcctt tgccaaaagc gtccaggaaa aggccgaatt
     4621 ccttggcaag cgccttgagg aaatcgccac ccgctatggc gacaatggca gcatctatcg
     4681 caagggccgt ggcctgatgc agggtatctg ctttgaaagc ggtgaaatcg ctgacaaagt
     4741 gacgaccgaa tgctttggcc gcaacctgat cattgaaacc agtggtccgg aagatgaagt
     4801 tgtcaaatgc ctggttccgt tgatcatttc gatggaagat ctggcttatg gtcttgatat
     4861 tcttgaagtg tctaccgcca agattatggg caaaaacgtc gtcacggcca aggctgccgc
     4921 tgaataatat acataagggg tgacggtgcc tgccgtcacc cgcgccaccc tattttatta
     4981 aacagatcaa agagttggaa atgattgttc gtaccctgaa agaatgtgaa gaatccggtc
     5041 gtcgcgttgt tgccgataac tgggaaagca cccgcttgtc gctggccgat gatggcatgg
     5101 gtttttcatt tcacatcacc accatctatg ccggtacgga aaccgaaatc tgctatgcga
     5161 accattttga aacggtttac tgcatttcgg gcaatggtga agttgaaacc attgaagatg
     5221 gcaagaaata caagatcgta ccgggtacgg tttacattct gaacaagcat gaccgccact
     5281 atctgcgggg cggcacggaa gacatggttg ttgcctgcgc attcaacccg ccgcttaacg
     5341 ggcgtgaaac gcacgacgaa aacggcgttt atccgctcga tggcgaagca cttgccagct
     5401 aatggccttt tggatggggc aggaatgaca ttgcatccct gccccatccc gcacgacgag
     5461 aaactttaaa ggaaacggcc cccaaaggag gcaccatggt ttcagcccgt aaacaagcgg
     5521 atgccggaca tagcgtccac aagatcggtg gaacgtccat gacgcaggtt caggctgtga
     5581 tggataatat tctggttggt gatcgcgacg agagcgatct ttataaccgg attttcgttg
     5641 tttcggccta tggcggattt accgacctgc tgcttgaaaa caagaaaacc ggcgaggccg
     5701 gcgtttatca actttatgcc ggttcggaaa ccgactgggc atggggtgat gcgcttaaca
     5761 aggtcgccga tcgaatgtgc gccctgaatg aagaaatctt ccaggatcag aatgacctga
     5821 aactggcaaa ttcctttgtc caggaacgcg ttgaaggcac gcgtagctgc ctgatggatt
     5881 tgcatcgcct atgcagcttt ggccatttca agctggaaaa gcatttgctg accgtgcgtg
     5941 aaatgctaag cgccatcggc gaagcccata gcgcgcacaa caccatgctt ctgttgcgtg
     6001 gcaaaggcgt gaatgcggta tttgccgacc tgtctggttg gcgcgaaccc gaagcatcga
     6061 cacttgatga acgcatcaac agcgtttttg aaaatatcga tgtcaaatcg caaatgccga
     6121 tcgtgaccgg gtatgcccag tgcagcgaag gcttgatgag catttatggc cgcggctata
     6181 gcgaagtgac cttttcgcgt attgcctgcc tgacgggggc gcatgaagcc atcattcaca
     6241 aggaattcca cctgagcagt gccgacccgc gcattgttgg tgaagatgcc gtgcgcccga
     6301 ttggccgtac cagctatgac gttgctgatc agctttcaaa tatgggtatg gaggccattc
     6361 atccgcgtgc tgccaaaggc ttgcgccaga atggtattcc gctgcgcatt gccaacacgt
     6421 ttgagccgga acatccgggt accgtgattg acgatgactt tcatcctgat gaaagccgcg
     6481 tcgagatcgt taccggtatt ggcacgtcgt tcgagatcga ggtgttcaac caggatatgg
     6541 ttggtgtacc gggtgccgaa gaagaagtgc tgagcgttct gaaacgttac aaggttccga
     6601 ccgttaccaa gaacctgaat gcgaatacca ttacgcatta tgtttcggca ccgcttaagc
     6661 agatccgtcg ctgcgtggaa gaaattgaga gccgccagcc ggaggcatcg gttaaaaccc
     6721 gcaaggtagc gattgtatcg gtcattggtg caaatatcag ccagccgggc ctgtttgccc
     6781 gtgcggtcag tattcttgat gatgctggtg ttgaactggt tgccagccat tcgcccagcc
     6841 gccgtgttga tttgcagttt gttgttgccg aaggcgactt caacaaggcg atcatcgccc
     6901 tgcatcgcgg cctggtggaa ggcaaaaaat cagccaccgc caccaagacc aaaaacagca
     6961 aggatgatgg cgacaatgtt ggtgaactgg ctgcggcctg acgccaacac cctgttcaca
     7021 tcatgctgaa tttaaaggcc cgcagatgat tttgcgggcc ttttttgtat ctggcctttg
     7081 tgtgacttga agtcagtcaa agatcacggt accagcaata accccgttct tttgcatcat
     7141 ccccgaacca agcggagccc agcccatgtc agccaccagc aaccgccctg ccattgacca
     7201 gttattgcag gtcatgaagc aattgcgtga tcccgaaaat ggctgcccgt gggacattga
     7261 acaggatttt tccaccatcg ccccctacac catcgaagaa gcctatgaag tggcggatgc
     7321 gattgccaat aacgatatgg tggagttgcg tgaagaactg ggtgatttgc tgttgcaggt
     7381 ggtttttcat tcgcaaatgg ctgaagaaca aaaactgttt tcgttcaacg atgttgccca
     7441 aagcatcaat gataaaatga ttgcccggca cccgcatgtg tttggcgaaa ttgatgccaa
     7501 aaccgccgat catgttaatc agaactggga aaagctgaaa gccgccgaac gccagcgcaa
     7561 ggcggaccgc aaaggcaatg aaacgccaag cgcgcttgat ggtgtggcat ccgccctgcc
     7621 agcgctgatg cgggccgaaa aactgacgaa gcgtgcggca cgtgttggat ttgactggcc
     7681 cgatattgaa caggtgtttg acaagctgag tgaagagatc ggtgaattgc gcgccgagct
     7741 gacagaaaac ccggtgcagg accgcctgga ggatgaattg ggcgacatgc tgtttgtgat
     7801 ggcaaatctt gcccgcaaaa tgcaggtgga cccggaagtt gccctgcgcc gtgcgaacca
     7861 caaattcacc cggcgctttc aattcatcga aaaggagttg gccgggcgcg gcaaaacacc
     7921 ggggcagtcc agccttgagg aaatggatgc actttggaat gcagccaagg tcgaagaacg
     7981 aaaaacaact accaaaccgc aggaataatt ttcacattcc actttcaaca aataccacaa
     8041 tgcattgttt tatttaaatg atgaggcctt tttataaatg acagccagtt acgtcgccat
     8101 tgtcctgctg ggggcgttcg ctggtgggtt tgtaaatggc ttggccgggt ttggtacggg
     8161 ccttgtggca ctgggcatct ggcttcatgt gattgatccg ctttatggcg caccactggt
     8221 ggcgatttgt tctgttgccg ggcagttgca aacattgccg gcaatctggc atgcggtgaa
     8281 tatccgccgg ttattgccaa tgctggtggg cggggttttg ggtattccag ttggtatcct
     8341 gcttttgggg catatggcgc cggatctgtt tcgcctgtcc ctggggctgt ttttgatcgc
     8401 ctttgccatt ttcatgatgt tcaaccggtc actaccggcg atgcgttggg gcagcaaaaa
     8461 gaccgaaggc atggtggggt tttgtggcgg gatattgggt ggtctggcgg ggctttccgg
     8521 gccgctgcca accatatggg taaccctcaa acgctgggaa aaggatgaac ggcgcagcgt
     8581 ttttcagatg ttcaatttga ccattctggc ctgtgttgcc atttcacatc tagcaattga
     8641 cgggataaca gttgcgtttt cccaattggt gttaatcgcc ctgcccggca catttgttgg
     8701 tgcctttgcg ggcatccggc tttatcgacg gttaaacgac catcgttata atctgctggt
     8761 tctgattatg ctggcgatat ccgggataac actggtggtt tcggaaatcg gctaacatat
     8821 agcttagctg gctttctttc caaacacggg caaaagcaac gtattcacct catcccacag
     8881 ctcggccgag gatgcgcaga tcaatgccgg gccatcacca tttgcctgat aatcacgaaa
     8941 tacgccatca atcgaaatat aacgggcaat accgccagcc tcgctgacca ataaggtgcc
     9001 aggggcatga tcccacggtt taagggtcct ataggcgcaa aaatcgtaat taccctgggc
     9061 gatatcctca tattcggcgc cacagcagga atagtgcgat agtttttcaa aaaaattatg
     9121 atgcccgtgc gcctttacca gccggtcgcg ggcataggaa ataaggaagc cacgggctga
     9181 tgcagcagac gtaacatcac tgcgaaccgt gacctgtttt ccattatgga aggtgccact
     9241 gcccttttcc gcaacggtga catcacccgt gatcgggtcc acgatccagg cggcgatggt
     9301 aacgccatcg cgataaagag ccagcatgga tcgaaacggt tggcgctgat gggcaaagtt
     9361 tttcgtgcca tccaccgggt caatgatcca gattggggca ttgtcagcaa aaacacggcc
     9421 ctgcaaacca gcatcatggt gggcggcttc ctcgcccagc gcgacactgc ccggaagcag
     9481 tgccgttaat cgctgggtca ggatgcgttc tgcctcggta tcggcaatgg ttaccagatc
     9541 ctgggcatgg gttttgatgt cgatatcacc ggcatcaagc tgtccgaatc gtggcgcaat
     9601 ttcctcggcg gcgacttcac ggataatggt tgttaccttg tctatatccg gtgtcatcac
     9661 atcggttttc ctgcgttgtt ttcgggttag agggaataaa gttccagtcc gtttttctgg
     9721 caccagtcct cgaaccgacc gagacgtgtt tcatcaaggc ggatttcaat actgataccg
     9781 tcattgcctt caatgcggtt caaaacctca ccctgctgat aaagcccggc cagggcctta
     9841 ccattggcat aaggtaccag aacctgaaag accggcattt cagccgtcag acggttttca
     9901 accagacgca acagattatc gcagttttcg cctgtcaggg ccgaaacgac gatggtgttg
     9961 tcctggcggc tgctttgttc aagcacggca aggcggtctt cttcatcaag aagatcggcc
    10021 ttgttcatgg cttcaaccag ttcagccccg tcaatcacct gctgcagccc cagttccttg
    10081 agaacttcaa gcacgtcctg cttttgcgca tcggtttcag gtgatgaagc atcacgcaca
    10141 tgcacaatca gatcggcttc cagcacttct tccagggttg cacggaaggc ggcaacaagg
    10201 tcgtgcggca gatcggaaac gaaacccacc gtgtcagaca ggatcacctt gcggccgctg
    10261 ggaagttcca gcccgcgcat ggtggggtcc agtgtggcaa acagcatgtc ttcggcaaaa
    10321 acctcggact gggtcagccg gttaaacagc gttgatttcc cggcattggt ataaccaacc
    10381 agggcgacaa tcggataagg aa
//
