LOCUS NZ_CP024199 20846 bp DNA circular CON 03-MAY-2024 DEFINITION Thalassospira marina strain CSC3H3 chromosome, complete genome. ACCESSION NZ_CP024199 VERSION NZ_CP024199.1 KEYWORDS RefSeq. SOURCE Thalassospira marina ORGANISM Thalassospira marina Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodospirillales; Thalassospiraceae; Thalassospira. REFERENCE 1 (bases 1 to 4530245) AUTHORS Dong,C., Liu,R. and Shao,Z. TITLE Biodiversity and function of Thalassospira species in the particle-attached aromatic-hydrocarbon-degrading consortia from the surface seawater of the China South Sea JOURNAL Unpublished REFERENCE 2 (bases 1 to 4530245) AUTHORS Dong,C., Liu,R. and Shao,Z. TITLE Direct Submission JOURNAL Submitted (25-OCT-2017) State Oceanic Administration, The Third Institute, Room 329, Daxue Road 184, Xiamen, Fujian 361005, P.R. China COMMENT ##Genome-Assembly-Data-START## Assembly Method :: SMRT Analysis v. 2.3 Genome Coverage :: 300 Sequencing Technology :: PacBio ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI RefSeq Annotation Name :: GCF_002844375.1-RS_2024_05_03 Annotation Date :: 05/03/2024 04:06:34 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline (PGAP) Annotation Method :: Best-placed reference protein set; GeneMarkS-2+ Annotation Software revision :: 6.7 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total) :: 4,760 CDSs (total) :: 4,670 Genes (coding) :: 4,656 CDSs (with protein) :: 4,656 Genes (RNA) :: 90 rRNAs :: 7, 6, 6 (5S, 16S, 23S) complete rRNAs :: 7, 6, 6 (5S, 16S, 23S) tRNAs :: 67 ncRNAs :: 4 Pseudo Genes (total) :: 14 CDSs (without protein) :: 14 Pseudo Genes (ambiguous residues) :: 0 of 14 Pseudo Genes (frameshifted) :: 2 of 14 Pseudo Genes (incomplete) :: 13 of 14 Pseudo Genes (internal stop) :: 4 of 14 Pseudo Genes (multiple problems) :: 4 of 14 ##Genome-Annotation-Data-END## ##antiSMASH-Data-START## Version :: 8.dev-cf2fc5ee(changed) Run date :: 2025-09-12 22:03:19 NOTE :: This is a single region extracted from a larger record! Orig. start :: 2014066 Orig. end :: 2034912 ##antiSMASH-Data-END## REFSEQ INFORMATION: The reference sequence is identical to CP024199.1. Bacteria and source DNA available from Dr. Zongze Shao; The Third Institute of State Oceanic Administration, Daxue Road 184#, Xiamen, Fujian, 3610015, China, Tel: 86-592-2195321. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ COMPLETENESS: full length. FEATURES Location/Qualifiers region 1..20846 /candidate_cluster_numbers="1" /contig_edge="False" /product="terpene" /region_number="3" /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)" /tool="antismash" cand_cluster 1..20846 /candidate_cluster_number="1" /contig_edge="False" /detection_rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)" /kind="single" /product="terpene" /protoclusters="1" /tool="antismash" protocluster 1..20846 /aStool="rule-based-clusters" /category="terpene" /contig_edge="False" /core_location="[10000:10846](+)" /cutoff="20000" /detection_rule="(PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)" /neighbourhood="10000" /product="terpene" /protocluster_number="1" /tool="antismash" proto_core 10001..10846 /aStool="rule-based-clusters" /tool="antismash" /cutoff="20000" /detection_rule="(PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)" /neighbourhood="10000" /product="terpene" /protocluster_number="1" gene 1026..1793 /gene="surE" /locus_tag="CSC3H3_RS09200" /old_locus_tag="CSC3H3_09195" CDS 1026..1793 /EC_number="3.1.3.6" /GO_function="GO:0008252 - nucleotidase activity [Evidence IEA]" /codon_start=1 /gene="surE" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007089629.1" /locus_tag="CSC3H3_RS09200" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CSC3H3_09195" /product="5'/3'-nucleotidase SurE" /protein_id="WP_101284648.1" /transl_table=11 /translation="MIDLHNARILICNDDGIDAPGIKLLESLARQFSDDVWVVAPSIEQ SGAGHSLTLRRPLRIHQRDERHFAVDGTPTDCILLALQVIMRETPPDIVFSGINRGGNL GEDVTYSGTVAAAMESALLNVPAIAFSQYFSQKMISWDIARAHLTNVVTSLVKEEWPRN VLINVNFPEKEREGGAKIRITRQGQRKIGDHVAERQDPHGEPYYWIGAIRSELPEDENA DLHVIEKGDISVTPIGLDFTDNVTLEKLTKAFQ" gene 1805..2443 /locus_tag="CSC3H3_RS09205" /old_locus_tag="CSC3H3_09200" CDS 1805..2443 /EC_number="2.1.1.77" /GO_function="GO:0004719 - protein-L-isoaspartate (D-aspartate) O-methyltransferase activity [Evidence IEA]" /GO_process="GO:0036211 - protein modification process [Evidence IEA]" /codon_start=1 /gene_functions="biosynthetic-additional (rule-based-clusters) PCMT" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_011384900.1" /locus_tag="CSC3H3_RS09205" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CSC3H3_09200" /product="protein-L-isoaspartate(D-aspartate) O-methyltransferase" /protein_id="WP_172963462.1" /sec_met_domain="PCMT (E-value: 7.5e-61, bitscore: 203.4, seeds: 9, tool: rule-based-clusters)" /transl_table=11 /translation="MARAPEIDALLETLRQDGIHDEAVLKALADIPRELFVAEPFASRA WENVALPISKGQTISQPYIVALMTQALQLNDRMKVLEVGTGSGYQAAILARLARRVYTL ERHKPLLREAEDRFRKLGLHTISTLHADGGLGWKAQAPFDRIIVTASAQDVPPVLVDQL AIGGIMVVPVGEVSHTQILLRVMRTPTGIDVTELMPVRFVPMLAGTEEF" gene 2514..3665 /locus_tag="CSC3H3_RS09210" /old_locus_tag="CSC3H3_09205" CDS 2514..3665 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_008890994.1" /locus_tag="CSC3H3_RS09210" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CSC3H3_09205" /product="peptidoglycan DD-metalloendopeptidase family protein" /protein_id="WP_245881352.1" /transl_table=11 /translation="MMKNYVKHVVHNVRTLLFVCGTTAALSGCLLNSKPVPVVYDEGTN YSLSAMPELRNGERVITVKRGDSVSLLAERYHADFRKFAARNSLSSPYVIYPGQRLVLP PWQAEYDSRPPETAVAQSSGNSRATKNVVIAGRAQPVEITSAPLDAPSSAASQPASAAP ASDLSPVTDITSSKSTVIAGANLPVPGIKPQDIAAQAERQFASGNSWRNTTGTKVASSS PTSTGGSAAVVPSSAASHPSRVKVDDIVPAGRQGFIWPVQGKVVLGYGAGPGGLFNDGI NIAAERGTAILATDNGVVTYVGNELRGFGNLILIKHADGYVSAYAHTENPLVARGDVVS RGQRISSVGATGAVNRPQLHFEIRQGRKSRDPVKYLPQAVASR" gene complement(3749..4612) /locus_tag="CSC3H3_RS09215" /old_locus_tag="CSC3H3_09210" CDS complement(3749..4612) /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_009539876.1" /locus_tag="CSC3H3_RS09215" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CSC3H3_09210" /product="ATP-binding protein" /protein_id="WP_101286174.1" /transl_table=11 /translation="MTDTLADLLPTLSRIADALERMAPPARQQNDLSVADAFVWHAETG YLQPVKQVNRVDIGLLQGIEQQKRILYNNTKRFADGLPANNALLWGARGTGKSSIVKAM HALINMETPSSLALVEIHREDIPTLPVLLKILQDSGRKTVLFCDDLSFDLQDESYKSLK AVLDGGIEGRPANVIFYATSNRRHLMARDMIENERSTAINPSEAVEEKVSLSDRFGLWL GFHNISQDVYFEIIAGYAKEYDLDIAEDELFARAKEWTVTRGSRSGRVAWQFIQEIAGE QGKHIA" misc_feature complement(4728..4743) /note="TFBS match to CRP, Cyclic AMP receptor protein, confidence: strong, score: 19.26" /tool="antismash" misc_feature 4896..4914 /note="TFBS match to DmdR1, Iron(II)-dependent repressor, confidence: weak, score: 24.44" /tool="antismash" gene 4914..5303 /gene="yajC" /locus_tag="CSC3H3_RS09220" /old_locus_tag="CSC3H3_09215" CDS 4914..5303 /GO_component="GO:0005886 - plasma membrane [Evidence IEA]" /GO_function="GO:0015450 - protein-transporting ATPase activity [Evidence IEA]" /GO_process="GO:0009306 - protein secretion [Evidence IEA]" /codon_start=1 /gene="yajC" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007089625.1" /locus_tag="CSC3H3_RS09220" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CSC3H3_09215" /product="preprotein translocase subunit YajC" /protein_id="WP_101265798.1" /transl_table=11 /translation="MLISPAFAQGAGGAAGGADMLTSFLPLILIFVVFYFLLIRPQQKK QKEHKAMLAAVRRGDKIVTAGGIIGTVAKVVNDDEVSVEIADGVKVKVARGMISNVLSR TEPAKGGSDAEDKADEKKTEEVKKD" gene 5451..7022 /gene="secD" /locus_tag="CSC3H3_RS09225" /old_locus_tag="CSC3H3_09220" CDS 5451..7022 /GO_component="GO:0031522 - cell envelope Sec protein transport complex [Evidence IEA]" /GO_function="GO:0015450 - protein-transporting ATPase activity [Evidence IEA]" /GO_process="GO:0043952 - protein transport by the Sec complex [Evidence IEA]" /codon_start=1 /gene="secD" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007089624.1" /locus_tag="CSC3H3_RS09225" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CSC3H3_09220" /product="protein translocase subunit SecD" /protein_id="WP_101265796.1" /transl_table=11 /translation="MLYFTKWKATLVLLVCALGVIYAAPNFLPKGTFPTESSWMPGKQI SLGLDLRGGSHMLLEVGVDSVIRERYDSLTENMRDALREQRIRYRGLRGSADGASVTIV SDDDVEKARSLMAKLDPLTVVGGEGNHIMLTYNEQTRREMVDRAISQSLEVVRRRVDEL GTTEPSIQRQGEDRIVVQVPGLDDPSRLRDILGRTAKMNFHLINETKTPQQAKATGIPP GAVIMPGADNASPGEPEEYLVDRRVVVSGDNLIDSQPTFNDGRPVVSFRFDAAGGARFG DVTSKNTGRRLAIVLDGKVISAPRINEPIMGGSGIITGQFGVQEANDLSLLLRAGALPA PMKILEERSVGPGLGQDSIDSGEIAAVLGMVFVVAFMAVSYGLFGIFANLALIINLVLI LAIMSVLQATLTLPGIAGIVLTVGMAVDANVLIFERIREERKIGRSIISSIDAGYRSAM STILDANITTLIAALVLFSFGSGPIKGFAVTLAIGIITSMFAAIWVTRLIVAFWVKHRR PTELVL" gene 7042..7980 /gene="secF" /locus_tag="CSC3H3_RS09230" /old_locus_tag="CSC3H3_09225" CDS 7042..7980 /GO_component="GO:0031522 - cell envelope Sec protein transport complex [Evidence IEA]" /GO_function="GO:0015450 - protein-transporting ATPase activity [Evidence IEA]" /GO_process="GO:0043952 - protein transport by the Sec complex [Evidence IEA]" /codon_start=1 /gene="secF" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_008890998.1" /locus_tag="CSC3H3_RS09230" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CSC3H3_09225" /product="protein translocase subunit SecF" /protein_id="WP_101265793.1" /transl_table=11 /translation="MKPLHLVPDDVNIPFLKIRKLFYIFSLSLVVLSGVLFFTKGLNFG IDFRGGILLEIKTDGPADIPGLRDNLGSLGLGDVAIQQFGEPDDVLIQLQRQDGDEQAQ MAALETVTKALGDGVSIRRSELVGPKVGDELKEAGLYSVVISLALIMIYIWFRFEWQFA VASVVALLHDVIITVGLFVISGIQFDLATLAAILTVAGYSINDTVVVFDRIREFMRKYR KMDLIELLDLSINTTLSRTVMTSLTTLLALIALFLFGGEAIRGFTFALIFGIVIGTYSS ICVASPLLVVLKLRRKIPEGEEANPEEANAL" gene 8121..8501 /locus_tag="CSC3H3_RS09235" /old_locus_tag="CSC3H3_09230" CDS 8121..8501 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007089621.1" /locus_tag="CSC3H3_RS09235" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CSC3H3_09230" /product="Mth938-like domain-containing protein" /protein_id="WP_245881353.1" /transl_table=11 /translation="MSMDITPMVASGRKIVSSYGDGLFRFGAEKAEGSVLLFPNEFMRW PHIDMTTVTEDSFADVIERAGQIDILLLGMGTRMQPVKTAWRNALRPHGIVIEPMDTGA ACRTFNVLLSEERRVAAALIAV" gene 8747..9955 /locus_tag="CSC3H3_RS09240" /old_locus_tag="CSC3H3_09235" CDS 8747..9955 /GO_function="GO:0008381 - mechanosensitive monoatomic ion channel activity [Evidence IEA]" /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_008891002.1" /locus_tag="CSC3H3_RS09240" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CSC3H3_09235" /product="mechanosensitive ion channel family protein" /protein_id="WP_101284649.1" /transl_table=11 /translation="MEEVTSGFEDLNDKVVAKFTDWADMVGGGDIGRAIVAIVILLFFI TIRKFLAHGVVGGIRRLADQHKHQTMAMILDALQTPIEVFVVLLGIYFSVEVMEFSDKT DAAIFNVLRVFVIATIFWAFYRVVEPLALGFNTFAGRFGAGLADDLRQFFIRCVRTLVV VLAGVALLEDWGIDVSAFLGGLGLAGMAVALAAKDSVANVFGGLTIFADKLFKRGDWIE TPHFEGVVEFVGLRATKVRTFSKAMVVMPNAEIVDSPVINWSRMTHRRIKMVIGVEYRT SADQIEKIIERLRNFLQQDEDVAQEDVPQMVHLADFGSSSINIDLYYFTNTTDWQEWRD IRNRHIIAFKRIIEEEGASFAFPSQSLYVESMPDMKMSRASANQDDQNKHQNQRQQTPE KMG" gene 10001..10846 /locus_tag="CSC3H3_RS09245" /old_locus_tag="CSC3H3_09240" CDS 10001..10846 /EC_number="2.5.1.-" /GO_function="GO:0004659 - prenyltransferase activity [Evidence IEA]" /GO_function="GO:0046872 - metal ion binding [Evidence IEA]" /GO_process="GO:0008299 - isoprenoid biosynthetic process [Evidence IEA]" /codon_start=1 /gene_functions="biosynthetic (rule-based-clusters) terpene: PT_phytoene_like" /gene_functions="biosynthetic (rule-based-clusters) terpene: phytoene_synt" /gene_kind="biosynthetic" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007089618.1" /locus_tag="CSC3H3_RS09245" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CSC3H3_09240" /product="phytoene/squalene synthase family protein" /protein_id="WP_101284650.1" /sec_met_domain="phytoene_synt (E-value: 1.8e-17, bitscore: 61.3, seeds: 8, tool: rule-based-clusters)" /sec_met_domain="PT_phytoene_like (E-value: 3.3e-16, bitscore: 57.2, seeds: 61, tool: rule-based-clusters)" /transl_table=11 /translation="MSDTPSLSYCADYVRRNDKDRFLCALFAPAEKREDLFTLYAFNQE ISKTREMVSEAMLGQIRLQWWRDALADIAKGEVRKHEVVEPLAGLIGQGRVSPATLEAM IDAREFDLFDNAPKDMAALENYIDATSGQLSEVAAGVLGAKSDDAARAARLAGNAYGLV GILRAMVFHGRAKRQYIPENVMEKHGVATGDIFEFRKTDAVKAMTHDLASRAKEKIREA RKMRQALPKLASPAALPIVLADNYLKKLKKAEYDPFSAEFGLARPASFRLTIRAVSGYW " gene 10955..11515 /locus_tag="CSC3H3_RS09250" /old_locus_tag="CSC3H3_09245" CDS 10955..11515 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_008891004.1" /locus_tag="CSC3H3_RS09250" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CSC3H3_09245" /product="hypothetical protein" /protein_id="WP_215907570.1" /transl_table=11 /translation="MKSIRNIALLVIIPVGVLITLWTFAKPAAATSIDPFLGVYHGTAI SEETHELKARDLDVTIKKTGNAFSIDWSTVIYKSDGREKASAISIDFFSTDRADIFGSA MRTGLFGKRVPNDPLKGQPFVWARIVGPTLTVHAMYILDDGGYEMQVYERTLDPHGNLD LVFKRFRNGKSIREITGELTRVK" gene complement(11628..12971) /gene="trmFO" /locus_tag="CSC3H3_RS09255" /old_locus_tag="CSC3H3_09250" CDS complement(11628..12971) /GO_function="GO:0050660 - flavin adenine dinucleotide binding [Evidence IEA]" /GO_process="GO:0008033 - tRNA processing [Evidence IEA]" /codon_start=1 /gene="trmFO" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007089616.1" /locus_tag="CSC3H3_RS09255" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CSC3H3_09250" /product="methylenetetrahydrofolate--tRNA-(uracil(54)- C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO" /protein_id="WP_101284651.1" /transl_table=11 /translation="METVTPVHIIGAGLAGSEAAWQLAEAGIPVIIHEMRPERPTDAHQ TDKCAELVCSNSFRSDDKEYNAVGLIHDEMRRANSLILRCADEHKVPAGAALAVDREGF SQAVTDALMNHPLISMERGEISGLPPAEWGNTIIATGPLTSGSLAAAILEQTGEQSLAF FDAIAPILYKESIDFDKAWFQSRYDKGDGKDYINCPMNKEQYNNFIDALLAGDKTEFKD WEKNTPYFDGCLPIEVMAERGRETLRHGPMKPVGLTNPHDPLVKSYAIVQLRQDNALGT LYNMVGFQTKLKYGAQVEVFKTIPGLENADFARLGGIHRNTFLNSPRLLDPTLRLKSRR DIRFAGQITGCEGYVESAAVGLMTGRFTAAEKQGRELPLPPAVTAMGALLGHITGGANE DSFQPMNVNFGLFPELDLKKRVKGRDRKKLYTDRAIPAFDEWLAEIGN" gene 13231..14055 /locus_tag="CSC3H3_RS09260" /old_locus_tag="CSC3H3_09255" CDS 13231..14055 /codon_start=1 /inference="COORDINATES: ab initio prediction:GeneMarkS-2+" /locus_tag="CSC3H3_RS09260" /note="Derived by automated computational analysis using gene prediction method: GeneMarkS-2+." /old_locus_tag="CSC3H3_09255" /product="hypothetical protein" /protein_id="WP_101284652.1" /transl_table=11 /translation="MTDPANTSSPNTPGTPNATGISPATSSAGPSNSISTPVAAVSGRA ADHPQTSGISGDSSATRPQNGTDASANPATPAGVTPVSPLNSFVAVLTGGGDVAKLSAA SLALVFSPLVLLAVLATFFTIDVLVSSWGGRLFGFSYDLVFFVGVFGALGATVSVMLYI SDTQNWQNLTPLDIFFRFLFRPFLGFVFAILALLIVRAGIVPDSLSQLQAIRDLNDLGT LKLSDESGQQVAIMLLIAFLAGFSERLVKAILQTVEGRIRALAIGSDSAKTT" gene complement(14095..16923) /gene="uvrA" /locus_tag="CSC3H3_RS09265" /old_locus_tag="CSC3H3_09260" CDS complement(14095..16923) /EC_number="3.1.25.-" /GO_function="GO:0003677 - DNA binding [Evidence IEA]" /GO_function="GO:0005524 - ATP binding [Evidence IEA]" /GO_function="GO:0016887 - ATP hydrolysis activity [Evidence IEA]" /GO_process="GO:0006289 - nucleotide-excision repair [Evidence IEA]" /codon_start=1 /gene="uvrA" /gene_functions="transport (smcogs) SMCOG1000: ABC transporter ATP-binding protein" /gene_kind="transport" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007089614.1" /locus_tag="CSC3H3_RS09265" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CSC3H3_09260" /product="excinuclease ABC subunit UvrA" /protein_id="WP_101284653.1" /transl_table=11 /translation="MLTKISVRGAKEHNLQNIDVELPRDSLVVITGLSGSGKSSLAFDT IYAEGQRRYVESLSAYARQFLEMMQKPDVEYIDGLSPAISIEQKTTSRNPRSTVGTVTE IYDYMRLLWARVGIPYSPATGLPIVSQTVSQMVDRVMEMEEGTRLYLLAPIVRGRKGEY KKELKDLRARGFQRVKIDGEMYDIDEAPDLNKKLKHDISVVVDRLVVKDGIQTRLADSF ETALELTDGIVFAEDARSGDSTLFSAKFACPVSGFTIEEIEPRLFSFNNPFGACPVCDG LGTQMEFDPELVVPDTSKTLDDGAIAPWASSTSKYYLQTLRAIAKHFGFKTNVAWDKLP EKAKHIILHGSGEEEVTVTYDDGSRSYKVTRPFEGVIPNMSRRWRETDSQWARDELAKY QTVSDCDACHGHRLKPEALAVKVDKCTISEITALSIGKAVEWFASLEPKLDEKQTEIAR RILREINDRLKFLSDVGLDYLSMSRTSGTLSGGESQRIRLASQIGSGLTGVLYVLDEPS IGLHQRDNDRLLETLKRLRDLGNTVLVVEHDEDAIRAADYVVDMGPGAGIHGGRIVAQG TPDEIQQNPESLTGKYLTGIEQIAVPAKRRDGNGKAITISGARANNLQNITVDFPLGRF VCVTGVSGGGKSSLVIETLYKSLAKRMHNARTQPGAHDSISGIEHIDKIIDIDQSPIGR TPRSNPVTYTGAFTPIREWFAQLPEAKTRGYKPGRFSFNVKGGRCEACQGDGVIKIEMH FLPDVYVTCDQCKGKRYNRETLEISFKGKSISDVLDMTVEEGADFFKAVPSIRDKMETL KQVGLSYVHLGQQATTLSGGEAQRVKLAKELSKRATGRTIYILDEPTTGLHFHDIRKLL EVLHTLVDQGNTVVVIEHNLDVIKTADWIIDIGPEGGDGGGELVSAGTPEDIMKNDRSY TGKYLKRHLRLS" gene complement(17121..18200) /gene="fbaA" /locus_tag="CSC3H3_RS09270" /old_locus_tag="CSC3H3_09265" CDS complement(17121..18200) /EC_number="4.1.2.13" /GO_function="GO:0004332 - fructose-bisphosphate aldolase activity [Evidence IEA]" /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]" /GO_process="GO:0005975 - carbohydrate metabolic process [Evidence IEA]" /GO_process="GO:0006096 - glycolytic process [Evidence IEA]" /codon_start=1 /gene="fbaA" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007089613.1" /locus_tag="CSC3H3_RS09270" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CSC3H3_09265" /product="class II fructose-bisphosphate aldolase" /protein_id="WP_101265781.1" /transl_table=11 /translation="MPSVKPGVVTGAAYRELVAACKQDGYALPAVNITTSSTLNAALEA AANAGSDIIIQLSNGGAQFFAGKGIKDANAARVIGAVSAARHTQLLAEYYGVAVVLHTD HANRKFVPWVDGMLSWGEKAYKETGKPLFSSHMLDLSEEPLEENLATCEEFLKRLSAVD MSLEIELGVTGGEEDGIGKELDTTQDDIDPRLYTRPEEVEEAYRRLKPLGHFSVAAAFG NVHGVYKPGNVKLRPEILLESQKYVADKHGLDGFPLDFVFHGGSGSEKEKIEEAVGYGV FKMNIDTDTQFAYATAVGKYVEENDRAFKYQVDPEDDKPYKKQYDPRVWVREAELSMAA RLEEAFKDLGATGKSIAKG" gene complement(18221..19294) /locus_tag="CSC3H3_RS09275" /old_locus_tag="CSC3H3_09270" CDS complement(18221..19294) /GO_function="GO:0016491 - oxidoreductase activity [Evidence IEA]" /GO_function="GO:0016651 - oxidoreductase activity, acting on NAD(P)H [Evidence IEA]" /codon_start=1 /gene_functions="biosynthetic-additional (rule-based-clusters) GFO_IDH_MocA" /gene_functions="biosynthetic-additional (rule-based-clusters) GFO_IDH_MocA_C3" /gene_functions="biosynthetic-additional (smcogs) SMCOG1079: oxidoreductase" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_020593771.1" /locus_tag="CSC3H3_RS09275" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CSC3H3_09270" /product="Gfo/Idh/MocA family oxidoreductase" /protein_id="WP_172963409.1" /sec_met_domain="GFO_IDH_MocA (E-value: 2.1e-25, bitscore: 87.6, seeds: 39, tool: rule-based-clusters)" /sec_met_domain="GFO_IDH_MocA_C3 (E-value: 1e-10, bitscore: 39.5, seeds: 4226, tool: rule-based-clusters)" /transl_table=11 /translation="MTSKPVRILVVGLGNMGMSHAKAYHALEGFEIVGLCTRSPIAPGT LPEGFESYPQFTDYEAALAQTGPDAVSINTYTETHANYAIAAFEAGAHVFIEKPLAATV VDAERVIESAQKHGRKLVIGYILRHHPSWEKFVELAKTLGKPLVMRMNLNQQSSGEFWE THKRLLNSVSPIVDCGVHYLDMMCLMTAAKPISVHAMGVRLSPDVAEDMYNYGQLQVRF DDGSIGWYEAGWGPMISETAFFVKDVIGPKGSVSITDVEEKGAGSADIDTHTKTNALKL HHAATDRHGKFSHSDEIIRTDDEPDHDGLCLREQQFFLKSITENLDLTQHMTDALGSLR IALAADQSVRTGNIIML" gene complement(19339..20472) /gene="nagA" /locus_tag="CSC3H3_RS09280" /old_locus_tag="CSC3H3_09275" CDS complement(19339..20472) /EC_number="3.5.1.25" /GO_function="GO:0008448 - N-acetylglucosamine-6-phosphate deacetylase activity [Evidence IEA]" /GO_process="GO:0006040 - amino sugar metabolic process [Evidence IEA]" /codon_start=1 /gene="nagA" /gene_functions="biosynthetic-additional (rule-based-clusters) Amidohydro_1" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007089611.1" /locus_tag="CSC3H3_RS09280" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="CSC3H3_09275" /product="N-acetylglucosamine-6-phosphate deacetylase" /protein_id="WP_215907571.1" /sec_met_domain="Amidohydro_1 (E-value: 2e-30, bitscore: 104.0, seeds: 57, tool: rule-based-clusters)" /transl_table=11 /translation="MTVLAIKNARLFDGEQFHDDACVIVENGKVSAVSPARIPDGADIF DARGNVLAPGFIDVQVNGGGGVLLNHTPSVDGIRQIMAGHRQFGTTAMLPTLITDTREK MLAAIEAVKNAIDEGVPGIVGIHLEGPYLNTERKGVHDANIIRPMEDDAIEVLSSLPNG RILVTMAPEKAKPGTIEKLCANGVLVCAGHTAGTYDDIQKAIGEGLRGFTHLFNAMSPL AHREPGVAGAAIADKNTWCGLIADGYHVHPAAMQVAVRAKQTGKIMLVTDAMPTVGAKT KRFVLGGEEIIAIDGRCALADGTLAGSDLDMISAVKNSVEMIGVDLGEALRMASLYPAA FLKLDDRKGKIMPGFDADMVLFDPQTFQVKHTWISGI" misc_feature complement(20686..20701) /note="TFBS match to ColR, Phenol-responsive regulator, confidence: weak, score: 21.14" /tool="antismash" ORIGIN 1 ggcaggcagc cctgaatgat cagcttgacc tgctgctgac atcctttccg aacatccttg 61 atgcatcggt gcccaagggc gcggacgaag atgacaatgt cgaaattcgt cgctggggca 121 ccccgggcga atttgacttt accccgggcg aacattttga cctgggcgaa aagctcggca 181 tgatggattt tgccacggcg gcaaagctgt cgggttcgcg ctttgttatt ctgcgcggtc 241 agcttgcgcg tctggaacgt gccctggcac agttcatgat tgacatgcat gtcaacgaac 301 atggctatga ggaaaccacc acgccgatgc tggtgaagga cgatgctatg tttggcactg 361 gccagttgcc gaaatttgcc gaagattcgt ttaaaacaac caatgacatg tggttgatcc 421 cgacatcgga agtcacactg accaaccagg ttgccggcga aatcgtcgat actgccagcc 481 tgccgttgcg ctataccgca ctgacccagt gtttccgttc cgaggcggga tcggccgggc 541 gtgatacgcg cggtatgatc cgccagcacc agttctcgaa ggtcgaaatg gtttccatcg 601 tcgatccgga acagtcggat gccgaactgg aacgcaaaac acagtgcgct gaagaagtgc 661 tgcagcgttt gggcctgcct taccgcacca ttttgctgtg ttcgggcgat accggttttt 721 cggccatgaa aacctatgac atcgaagtct ggttgccggg gcagggccgt taccgcgaaa 781 tttcgtcctg ctcgaacacg ggtgaatttc aggcacgccg catgaatgcc cgttgccgtg 841 cccagggcga caagaaaacc cgtttcgtcc ataccctgaa tggatcgggc cttgcggtcg 901 ggcgctgcct gattgcggtc atggaaaatt atcagcaggc agatggttcc attaaggttc 961 cggaagcatt ggtgccttat atgggcggta tgactgaaat ttccgtgacg aagtaaggtt 1021 tatccatgat cgatctgcat aatgcccgca ttctgatctg caatgatgac ggtatagatg 1081 cccctggcat caaattgctt gaaagtcttg cgcggcagtt ttcagatgat gtgtgggtgg 1141 ttgcaccgtc gatagaacaa agtggcgcag ggcattcgct taccctgcgc cgcccgttac 1201 gtatccatca gcgtgacgaa cgccattttg ctgttgatgg cacgccgacg gactgcatcc 1261 tgctggcctt gcaggttatc atgcgtgaaa caccacctga tattgtgttt tcaggcatca 1321 atcgcggcgg caatctgggc gaggatgtaa cctattccgg cacggtggcg gccgccatgg 1381 aatctgctct gctgaatgtg ccggccattg cattttcgca gtatttttcc cagaaaatga 1441 tcagttggga tattgccagg gcgcatttga ccaatgtggt tacttcactt gtgaaggaag 1501 aatggccgcg taacgttttg attaacgtta attttcctga aaaggaacgc gaaggtgggg 1561 cgaaaattcg cattacacgg caggggcagc gtaaaattgg tgatcacgtt gccgaacgtc 1621 aggacccgca cggcgaaccc tattactgga tcggtgccat tcggtcggaa ttgcccgaag 1681 atgaaaacgc cgatttacat gtgatcgaaa agggcgatat ttctgttacg ccaatcggcc 1741 ttgattttac cgataatgtg accctcgaaa aactgacaaa ggcatttcag tgagtacgcc 1801 tgccattgcc cgtgcaccgg aaatcgatgc cctgctggaa acactgcgtc aggacggcat 1861 tcacgacgaa gccgttctga aagcgctggc tgatattccg cgtgaattgt ttgttgccga 1921 gccatttgcc agccgcgcct gggaaaatgt cgcattgcca atcagcaagg ggcaaaccat 1981 ttcccagccc tatatcgtcg cgctgatgac acaggccctg cagcttaatg atcgcatgaa 2041 ggtgcttgag gtcggtacgg gatctggcta tcaggctgca atccttgccc gtctggcgcg 2101 ccgtgtttac acacttgagc ggcataaacc cctgctgcgc gaggcagaag accgttttcg 2161 caaactgggc ctgcacacca tttcaaccct tcatgccgat ggcgggctgg ggtggaaggc 2221 acaggcaccg tttgaccgta tcattgtgac ggcatcggca caggatgtgc caccggtact 2281 ggttgatcag cttgccattg gcgggatcat ggtggtgccg gtaggcgaag tttcccatac 2341 ccagattttg ctgcgtgtga tgcgcacccc gaccggcata gacgttaccg aactgatgcc 2401 ggtgcggttt gtgcccatgc tggctggcac cgaagaattc tgaaatcttg ccatctgtga 2461 aaattttcgc ctcgttaccg attattaacc ggtgatgcgg gaaaatcttc gacatgatga 2521 aaaattatgt caaacacgtc gttcataatg tccggacgtt gctgtttgtc tgcgggacaa 2581 cagcggcgct aagtgggtgt ttgcttaatt caaagccggt tccggtcgtt tatgacgaag 2641 gcacaaatta ttcgctttcg gccatgccag agctgcgcaa tggcgaacgc gtgatcaccg 2701 ttaaacgtgg tgacagtgtt tcgctgctgg ccgaacgcta tcacgccgat ttccgcaaat 2761 tcgccgccag aaacagcctt agctcgccgt atgtcatcta tcccggacaa cggctggttt 2821 taccgccctg gcaggccgaa tatgacagcc gcccgccaga aacggctgtg gcgcaatcat 2881 caggcaattc gcgagcaacc aaaaacgttg tcattgctgg ccgcgcgcaa ccggttgaaa 2941 taacctcggc accgcttgat gcgccttcat cggcagcatc acagcctgca tcggctgcac 3001 cggcatccga tcttagcccg gttactgaca tcaccagttc caaaagcacg gttattgccg 3061 gcgccaattt acctgttccg gggataaagc cacaggatat tgccgcacag gccgaacgcc 3121 agtttgcgtc gggaaatagc tggcgcaata ccaccggcac caaggttgcc agcagcagcc 3181 cgaccagcac gggtggtagc gctgctgttg ttccttccag tgcggcatcg cacccatcgc 3241 gggtcaaggt tgatgatatc gttcctgcgg gccggcaggg ctttatctgg ccggtacagg 3301 gcaaggttgt tctgggatat ggtgccgggc ccggtggcct gtttaatgat ggcatcaata 3361 ttgcggctga acgcggcact gccattctgg cgaccgacaa tggggttgtt acctatgtcg 3421 gcaatgaact gcgtggtttt ggcaacctga tcctgatcaa acatgccgat ggttatgtgt 3481 cggcctatgc gcacactgaa aacccgctgg tggcgcgcgg cgatgtggtt agccgagggc 3541 agcgcatttc ttcggtcggc gcgacgggtg cggtcaaccg accacagctt cattttgaaa 3601 tccgtcaggg tcgcaaatca cgcgatccgg taaaatactt gcctcaggca gttgcatccc 3661 gctgacccat taaaaaacgg cctgattgcg caatcaggcc gtttctgttt gggctgtcta 3721 tgagcattcg atgggcttgt atgcctgttc aagcgatatg tttgccctgt tcccctgcaa 3781 tttcctgaat gaactgccag gcgactcgtc cggaccggct gccacgcgtt acggtccatt 3841 ccttggcgcg cgcaaatagt tcgtcctcgg caatatccag gtcatattcc ttggcatatc 3901 cggcgatgat ctcgaaataa acatcctgcg aaatattgtg gaagcccagc cacagcccga 3961 accggtcgga aagcgatact ttttcttcaa ctgcttctga cgggttgatt gcagttgagc 4021 gttcgttttc gatcatgtcg cgcgccatca ggtggcgacg gttggatgtg gcatagaaaa 4081 tcacgttggc cgggcggcct tcaatcccgc catccagcac tgctttcaat gatttatagg 4141 attcgtcctg caggtcaaaa gacaggtcat cacagaacag aaccgtcttg cgcccgctat 4201 cttgcaggat tttcagcaaa acgggcaggg tgggaatatc ctcgcggtga atttccacca 4261 gcgccaggga agacggggtt tccatattga ttagcgcatg catcgccttg acgatcgagc 4321 ttttgcccgt accgcgcgcg ccccacagca gcgcattatt ggctggcagg ccatcggcaa 4381 agcgtttggt gttgttataa agaatacgct tttgctgttc gatcccttgc aaaaggccaa 4441 tatccacccg gttaacctgt ttgacgggct gcaaataccc ggtttcggca tgccagacaa 4501 aggcatcagc cacagaaagg tcgttctgct ggcgggcagg cggtgccatg cgttcaaggg 4561 catcggcaat gcgggacaat gttggcagca aatcggcaag ggtatcggtc atgggggcct 4621 cttggatgtt ttcatttttg gtggcagcag aattcactgc attttatggg cgaaccctag 4681 cacagcatgc ttcgcacgga atagtccgta taaaccggca ttgggactgt gaatctggtc 4741 acaaagtcgc gcaacacaag gtaattcaac cgccaatgcg catggatgtg tgatttataa 4801 ggcacgcaat ggcctgtttg aggcaaattt aagcgatctt tcttgcaacg gtgaacgaca 4861 ccgttatatt ccggcagttg attggttttt ctgttttagg ttaggagttc ctaatgttga 4921 tttcaccggc ttttgctcag ggtgctggtg gcgctgctgg cggagcagat atgctgacta 4981 gctttctgcc gctgatcctg atttttgtgg ttttctactt tttgctcatt cgtccgcagc 5041 agaagaaaca gaaagaacac aaagcaatgc tggctgcggt tcgccgcggc gacaaaatcg 5101 ttacggctgg tgggatcatc ggaaccgttg ccaaggtcgt caatgacgat gaagtttccg 5161 tcgagattgc tgatggcgtg aaagtcaaag tcgcacgggg catgatctcg aatgtcctgt 5221 cgcgcaccga accggccaag ggtggttcgg atgccgaaga caaggcagac gagaagaaaa 5281 ctgaagaagt caaaaaagac tgatttctgt caaagctaca ggcggaacct cccggttccg 5341 cctttctgca tattactggc ggtcggccgg gcaagtcctt ccttattatc ctgattgagc 5401 cctgagcccg atgtcgcaat gttccccaga catctctggt taaaggacac atgctttatt 5461 ttaccaaatg gaaagcaaca ctggttttgc tggtgtgtgc tttgggcgtg atatatgccg 5521 cgccaaactt ccttcccaag ggtacgttcc cgacagaatc aagctggatg cccggtaagc 5581 agatcagcct tggtcttgac ctgcgtggtg gctcgcacat gcttttggaa gtgggggtcg 5641 atagcgttat tcgagaacgt tacgactcac tgaccgaaaa catgcgagac gcccttcgcg 5701 agcagcgtat tcgctatcgc ggcctgcgcg gttcggccga tggtgcatct gtaaccatcg 5761 ttagcgatga tgatgtcgag aaagcccgtt ccctgatggc aaagctggac ccgctgacgg 5821 ttgtcggtgg tgaaggcaac cacatcatgc tgacttacaa cgaacagacg cgccgcgaaa 5881 tggtcgatcg ggccatcagc cagtcgctgg aagttgtccg tcgccgtgtg gacgaactgg 5941 gtacgacaga gccgtcgatc cagcgtcagg gggaagaccg catcgttgtt caggttcctg 6001 gtcttgatga tccgtcgcgc ctgcgtgaca ttcttggccg tacggcaaag atgaactttc 6061 atctgatcaa cgaaaccaaa accccgcagc aggccaaggc caccggcatt ccgcccggtg 6121 ccgtgatcat gcccggtgct gataacgcat cacccggcga accggaagaa tatctggttg 6181 atcgccgtgt cgtggtttcc ggtgacaacc tgatcgacag ccagccaacc ttcaatgatg 6241 gccgcccggt ggtttccttc cgctttgatg cggcaggcgg ggcacgtttt ggcgatgtca 6301 ccagcaaaaa taccggccgc cgtcttgcca tcgttcttga tggcaaggtg atttcggcac 6361 cgcgcatcaa tgaaccgatc atgggtggca gcggcatcat taccggccag tttggcgttc 6421 aggaagcaaa tgacctgtcg ctgctgctgc gtgccggtgc gttgcccgcg ccgatgaaaa 6481 ttcttgaaga acgttcggtt ggtccgggcc ttggtcagga ttccattgat tcgggcgaaa 6541 tcgctgctgt tcttggtatg gtctttgttg tggccttcat ggccgtcagc tatggcctgt 6601 ttggcatttt tgccaacctt gccctgatca tcaacctggt gctgatcctg gcgatcatgt 6661 cggttctaca ggcaaccctg acattgccgg gtattgccgg tatcgttctg acagtcggta 6721 tggcggttga cgccaacgtt cttatttttg aacgtatccg cgaagaacga aaaatcggcc 6781 gatcgatcat cagttcgatt gatgccggtt accgcagcgc catgtcgacc attcttgatg 6841 ccaacatcac gaccctgatt gcagcgctgg tgctgttcag ctttggctcc ggcccgatca 6901 agggctttgc cgtgacgctg gcaatcggga tcatcacgtc gatgttcgca gccatctggg 6961 tgacacgtct gatcgtggca ttctgggtca agcatcgtcg tccgactgaa cttgtcttgt 7021 gaggaaggga gcaattcgca catgaaaccg ttgcatcttg ttccggatga cgtgaacatc 7081 ccgttcctca aaatccggaa gctgttttac atcttctcgc tctcgctcgt ggttctgtcg 7141 ggcgtgctgt tcttcaccaa gggccttaat ttcggtatcg atttccgcgg cggtattctg 7201 ctcgaaatca aaaccgatgg tccggccgat attccgggat tgcgtgacaa tcttggcagc 7261 cttggcctgg gtgacgttgc cattcagcag ttcggtgaac ccgatgacgt gctgatccag 7321 ttgcagcgcc aggacggcga tgaacaggcc cagatggcgg cacttgaaac cgtgaccaag 7381 gcccttggtg atggtgttag tatccgccgc tccgagcttg tcggcccgaa ggttggtgac 7441 gaacttaagg aagcaggcct ttattcggtt gtgatctcgc ttgcgctgat catgatctat 7501 atctggttcc gctttgaatg gcagttcgct gtcgcctcgg tcgtggcact gttgcatgac 7561 gtgatcatta cggttggcct gtttgtcatt tccggcatcc agttcgacct ggcaacgctg 7621 gctgcgatcc tgacagtggc aggttattcc attaacgata ctgtggtcgt gtttgaccgt 7681 attcgcgaat tcatgcgcaa atatcgtaaa atggacctga tcgagctttt ggatctgtcg 7741 atcaacacca cgctgtcacg taccgtgatg acgtcgttga cgacgcttct tgcgctgatc 7801 gccctgttcc tgtttggcgg cgaagccatt cgtggtttca ccttcgccct tattttcggc 7861 atcgtgattg gtacatattc ctcgatctgt gtggcgtcgc cgctgctggt cgtgctgaag 7921 ctgcgccgca agatcccgga aggcgaagaa gccaaccccg aagaggccaa tgccctgtaa 7981 tagcggcagg cagtaatttc tgccggtact agacatttac aagaaggccg ggttcgtgcg 8041 aaaacgaccc ggcctttttt tatgcccgaa gcaggcgata gaacgtttct ggcatccagt 8101 caccaccaac gaggtttccc atgtcgatgg acatcacacc gatggtagca tcgggccgca 8161 aaattgtttc cagctatggg gatggcctgt ttcggttcgg ggcggaaaag gccgaaggct 8221 cggttttgct gtttcccaac gaattcatgc gctggcccca tatcgacatg acgaccgtga 8281 ccgaagactc atttgccgat gtcatcgaac gggcagggca gatcgatatt ttgttactcg 8341 gcatgggaac ccggatgcag ccggttaaaa ccgcatggcg taacgccctg cggccgcatg 8401 gtatcgtgat cgagcccatg gataccggtg ccgcttgccg taccttcaat gtgcttctgt 8461 ccgaagaacg ccgggttgct gctgccctga ttgcggtctg acggcccctt tttctatttt 8521 catacctgcc ggtatagcgg ctgttccgat ctgtttcgga acaatttgcc tcgccttgcg 8581 tgttgtatcc taagggatta acgcaaacag gcaggggata cctgcggccc aatcctttaa 8641 cccgtgcaga cgcgcgtgtt ttgatgcaat cgctagcagg ttgtgtctgt tcgtgtggtc 8701 ggtccattga ccacgataaa gggatttcag gccgtaaatc tggcatatgg aagaagttac 8761 ttcgggcttt gaagacttaa acgacaaggt tgtcgcaaaa ttcaccgatt gggccgatat 8821 ggttggcggc ggagatattg gccgcgccat tgttgccatc gttattttgc tgtttttcat 8881 caccattcgt aaatttctgg cgcatggtgt ggtcggtggt attcgccgcc tggccgatca 8941 gcataaacat cagacaatgg ccatgatcct cgatgcatta caaacaccga ttgaagtgtt 9001 tgttgtattg ttggggatct atttttctgt cgaagtcatg gagttttcgg acaagacgga 9061 tgccgcgatt ttcaatgttt tgcgggtttt tgttatcgcc accattttct gggcatttta 9121 ccgggtggtc gaaccactcg cccttggctt taataccttt gccgggcgtt ttggcgcagg 9181 cctggccgat gatttacgcc agttttttat ccgctgtgtg cgcacccttg ttgttgtgtt 9241 ggctggtgtt gccctgctcg aagattgggg cattgatgtc agtgcgtttt tgggtggctt 9301 gggcctggcc ggtatggcgg ttgcgctggc tgcgaaggat tcagttgcca atgtttttgg 9361 cggattgacg atttttgccg acaagctgtt taaacgcggt gactggatcg aaacaccgca 9421 ttttgaaggt gttgtcgaat ttgttggcct gcgggcaacc aaggtacgta ccttttccaa 9481 ggcaatggtc gtgatgccca atgccgaaat tgttgattca ccagttatca actggagccg 9541 catgacgcac aggcgcatca agatggtaat cggcgttgaa tatcgtacca gcgccgatca 9601 gattgaaaag atcatcgagc gattgcgtaa ttttctgcag caggatgaag acgtggccca 9661 ggaagatgtg ccgcaaatgg tacatttggc tgattttggt agcagttcga tcaatatcga 9721 tctgtattat ttcaccaaca ccaccgattg gcaggaatgg cgcgacattc gcaatcgaca 9781 tatcattgca tttaaacgga taatcgagga agaaggggca tcatttgcat tcccgtccca 9841 gtctttgtat gtggaaagca tgccggacat gaaaatgtcc cgtgcatcgg caaaccagga 9901 cgatcagaat aaacaccaaa accagcgaca acagacgcct gaaaaaatgg gatagtttcc 9961 catcgggcgc tgggcgtgac cggcggataa cttaatttcg atgagtgata caccaagcct 10021 ttcctactgt gccgattatg tacggcgaaa tgacaaggac cgttttttgt gcgcgctgtt 10081 tgcgccggcc gaaaagcgcg aagacctttt cacgctttac gcctttaatc aggaaatatc 10141 gaaaacccgc gaaatggtca gcgaagccat gctgggccag atccgcctgc aatggtggcg 10201 tgatgcgctg gctgatattg ccaagggcga ggttcgcaaa catgaggtcg tcgaaccgct 10261 ggcaggcttg attgggcagg ggcgtgtcag cccggcaacc ctggaagcca tgatagatgc 10321 ccgcgaattc gacctgtttg ataatgcgcc caaggatatg gctgcacttg aaaactatat 10381 tgatgcaaca tccggtcagt taagcgaggt tgcagctggc gttcttggtg caaaaagcga 10441 cgatgccgcc cgcgctgccc gacttgcggg taatgcctat ggtctggtgg ggatattgcg 10501 cgccatggtg tttcatggcc gggcaaagcg gcaatatatc cccgaaaatg tcatggaaaa 10561 gcatggtgtg gcgacaggag atatttttga atttcgcaaa accgatgctg taaaagccat 10621 gacacatgac cttgccagcc gggcaaagga aaaaatacgc gaagcacgca aaatgcgcca 10681 ggcattgccc aaattggcgt ctcctgcggc attgccgatc gttcttgccg ataactattt 10741 gaaaaaatta aaaaaggccg aatatgaccc gttttctgcg gagttcggcc tggcgcgacc 10801 tgccagtttt cgcctgacga tacgggcagt ttcgggatat tggtgatgca gtgtctttac 10861 aacgggcaaa aaggttataa atctatatac atcaaccggt tcctgacctg atttggctgg 10921 ttgtccggtg ttagaagccc gcggggtcca aaaaatgaaa agcatcagaa atatcgcact 10981 cttggtcatc atcccggtgg gtgttctgat cacgctatgg acctttgcaa agcccgcagc 11041 ggcaacctct attgatccgt tccttggtgt ttatcacggc acggccatat ccgaagaaac 11101 ccacgagcta aaggcccgtg accttgatgt aaccatcaag aaaaccggca atgccttttc 11161 gattgactgg tccaccgtga tttacaaatc cgatggccgg gaaaaggcat cggcgatcag 11221 cattgatttt ttcagcaccg accgggccga catttttggt agcgccatgc gtaccggcct 11281 gtttggtaaa cgcgttccca atgacccgct aaaggggcag ccctttgtct gggcgcgcat 11341 tgttgggcca acgctgacgg ttcacgccat gtatattctt gatgatggcg gctatgaaat 11401 gcaggtctat gaacgtacgc tggacccgca cggcaatctt gatctggtgt tcaaacgatt 11461 tcgcaacggc aaaagcattc gtgaaatcac cggcgagctg acccgggtta aataacgcaa 11521 ttccatcgcc aaattgccct cacgaaaaac gcccgcgcaa gctgcacggg cgttttgtct 11581 tgttgcatat tcagcactgg cttgggcaaa gacgatgcct tgatgcctta attgccaatc 11641 tcggccagcc actcatcaaa ggcgggaatg gcacggtcgg tatacagctt tttgcgatca 11701 cggcccttga cccgtttttt cagatcaagt tccgggaaca ggccgaaatt gacattcatc 11761 ggctggaagc tgtcttcgtt ggcaccgccg gtaatatggc ccagaagcgc acccattgcc 11821 gttactgccg ggggcagggg taattcgcgg ccctgttttt ccgctgcggt aaaacgaccg 11881 gtcatcaggc cgacagcagc actttcgaca tagccttcac acccggtgat ctgcccggca 11941 aaacggatgt cgcggcgtga tttaagccgc agcgtcgggt ccagcaagcg cggggaattg 12001 aggaaggtgt tgcggtgaat accacccagg cgggcgaaat cggcattttc aaggcccggg 12061 atggttttga aaacctcgac ctgggcgccg tatttcagtt tcgtctgaaa accgaccata 12121 ttgtaaaggg tgcccagtgc attgtcctgg cgcaactgca caatggcata ggatttcacc 12181 agcggatcat gcgggttggt caggccaacc ggtttcatcg ggccatggcg cagggtttca 12241 cgcccgcgtt cagccattac ttcaatgggc aggcagccgt caaaatacgg ggtgtttttt 12301 tcccaatcct tgaattcggt tttgtcaccg gccagcagcg catcaatgaa attgttgtac 12361 tgctctttgt tcatcgggca gttgatgtaa tccttgccat cgcccttgtc atagcgtgac 12421 tggaaccacg ccttatcaaa atcgatgctt tctttataca ggatcggcgc aatggcatcg 12481 aaaaatgcca gcgactgttc tccggtttgt tccagaatag ctgccgcaag cgaacccgat 12541 gtcagcgggc cggtggcgat aatggtattg ccccattccg cgggcggaag gccgctaatc 12601 tcgccgcgct ccatggaaat cagcgggtgg ttcatcagcg catctgtaac ggcctgcgaa 12661 aagccttcac ggtcaacggc caaggcggca ccagccggca ccttgtgttc atcagcacag 12721 cgcagaataa gcgaatttgc ccggcgcatt tcatcatgga tcaggccaac agcgttatat 12781 tccttgtcat cggaacggaa cgaattggaa cagaccagtt cggcgcattt gtcggtctgg 12841 tgggcatcgg tcgggcgttc cgggcgcatt tcatgaatga tcacgggaat gccggcttcg 12901 gccagctgcc aggcggcttc actgccagcc aagccagcac cgatgatatg aacaggtgtt 12961 accgtttcca agctgaacct ccgtcagaac aggttttggc atcaggccaa gtttggcccc 13021 acacatagcg gccccggcac ccaaatatca agtcaattgc agcaatttgc cggtatcgcc 13081 gtgcgatttc ggttgggttt gggcttcttc gtcacatttg gcagcaccat ccgtcaaacg 13141 ggtattgaaa agtggtccgg tgcctgtgtt ctcaaatggc ttgtttgctg cacttaaccg 13201 gcttcaatgg ctttaaccag gtaaaaacaa atgaccgatc cggcaaacac cagttccccc 13261 aatacccccg gtacccccaa tgcaacaggt atcagcccgg caacatcatc cgccgggccg 13321 tcaaacagta tttcgacgcc cgtcgccgcc gtctcagggc gggcggcaga tcacccccaa 13381 acatctggca tatccggcga tagcagtgcg acgcgcccgc aaaatggcac cgacgcatct 13441 gccaatcccg caacgccagc aggggttaca cccgtttcgc ctctcaacag ctttgtcgcg 13501 gttttgaccg ggggtggtga tgtggccaag ctttcggcgg caagcctggc tttggtcttt 13561 tcgccgctgg ttctgcttgc ggtgctggca acgttcttta ccatcgatgt gctggtcagt 13621 tcttggggcg ggcggttgtt tggtttcagc tatgatctgg tattttttgt cggcgtcttt 13681 ggcgcattgg gcgcaacggt cagcgtgatg ctgtatatca gcgataccca gaactggcag 13741 aacctgacac cgcttgatat tttcttccgc tttttatttc ggccgttttt ggggtttgtt 13801 tttgcgattc tggcattgct gattgtgcgt gccgggatcg ttcccgacag tctgagccaa 13861 cttcaggcca ttcgggatct caatgatctg gggacgctca agcttagtga tgaaagcggc 13921 cagcaagtcg cgatcatgct tttgattgca tttttggcag ggtttagcga acggctggtc 13981 aaggccattt tgcaaacagt cgaaggccgc atccgtgccc tggcgatcgg ttccgatagc 14041 gccaaaacaa cctgatgcgg cccaaatcca cttaccaagt ccgtttacac gggtttatga 14101 aaggcgcaaa tggcgcttca gatatttgcc ggtatagctg cggtcatttt tcatgatgtc 14161 ttcgggtgta ccggcactaa ccagttcacc gccgccatca ccaccttcag ggccgatatc 14221 aataatccag tcggcggttt tgatcacatc caggttatgt tcaatcacaa caaccgtatt 14281 tccctgatcc accagggtat gcagcacctc aagcaattta cgaatgtcgt ggaaatgaag 14341 gccggttgtc ggctcatcca gaatgtaaat tgttctgcct gtcgcgcgtt ttgacagttc 14401 cttggcaagt ttgacacgct gtgcctcacc gccagaaagg gtggtcgcct gttggcccag 14461 gtgaacgtag ctcagaccca cctgttttag tgtttccatc ttgtcacgga tgctgggaac 14521 ggctttgaaa aagtccgcac cttcctcaac cgtcatgtca agaacgtcag atatcgattt 14581 gcctttaaac gatatttcga gagtttctcg gttgtatcgt ttacctttgc actgatcaca 14641 tgtaacatag acatcgggca aaaagtgcat ttcaattttg ataacgccat caccctggca 14701 ggcttcgcac cggccaccct tgacgttgaa tgaaaaccgc cccggtttgt agccacgggt 14761 tttggcctct ggcaactggg cgaaccattc acgaatgggg gtaaaggccc cggtataggt 14821 aacaggattg gaacgcgggg tacggccaat cgggctttgg tcaatatcga taattttatc 14881 gatatgttcg atgccggaaa tgctgtcatg ggcgccgggc tgggtgcggg cattatgcat 14941 acgtttggcc agcgacttat atagcgtttc aatcaccagg ctggatttac cgccacctga 15001 aacaccggtt acgcaaacaa acctgcccag cggaaaatca accgtgatgt tttgcaggtt 15061 attggcccgc gcaccgctga tggtaatggc cttgccattg ccatcgcgcc gtttggccgg 15121 aaccgcaatc tgttcaatcc cggtcagata tttgcccgtc aggctttccg gattttgctg 15181 aatttcatct ggcgtgccct gggccacaat acggcccccg tgaataccgg cacccggtcc 15241 catatcaaca acgtaatcgg cagcacggat ggcgtcttca tcatgttcga caaccagcac 15301 cgtattaccc aaatcgcgca gacgtttcag ggtttccagc aggcggtcat tatcacgctg 15361 atgcaggccg atggagggtt cgtcaagcac atataaaacg cccgtcaggc ccgagccaat 15421 ttgcgatgcc aggcgaatac gctggctttc cccacccgaa agcgtgcccg atgtacgcga 15481 catggaaaga taatccagtc ccacatcgct caggaatttc aggcgatcat taatctcgcg 15541 cagaatacga cgggcaattt cggtctgttt ttcatccagt ttcggctcaa gggatgcaaa 15601 ccattcaacc gccttgccaa ttgaaagggc ggtgatttcg gatatggtgc atttatcaac 15661 cttgaccgcc agggcttccg gcttcaggcg atgcccatgg caggcgtcac aatcggaaac 15721 ggtctgatat tttgccagtt catcgcgggc ccactggctg tcggtttcgc gccagcgacg 15781 cgacatgttg ggaataaccc cttcaaaggg gcgtgttacc ttatagctgc gcgaaccgtc 15841 atcataggtg acagtcactt cttcctcgcc cgaaccatgc aaaataatgt gcttggcttt 15901 ttccggcagt ttgtcccacg ctacgttggt tttgaagcca aaatgcttgg caatggcgcg 15961 cagcgtttgc aggtaatatt tcgacgtgct gcttgcccat ggggcaattg caccgtcatc 16021 cagcgttttg ctggtatcgg gcaccaccag ttcggggtcg aattccattt gtgtgccaag 16081 gccatcacaa accgggcagg cgccaaacgg gttgttgaac gaaaacaggc gcggttcaat 16141 ttcttcgatg gtgaaacccg aaaccgggca ggcaaatttt gctgaaaaaa gcgtgctgtc 16201 accggaacgg gcatcttcgg caaatacgat accgtcggtc aattccagtg cagtttcaaa 16261 gctgtcggca agacgcgtct ggatgccgtc cttgactaca aggcggtcga cgacgacgga 16321 aatatcgtgt ttcagctttt tgtttaggtc gggggcttcg tcgatgtcat acatttcgcc 16381 atcgattttc acacgctgaa aaccacgtgc gcgaaggtcc ttcaattcct ttttatattc 16441 gcccttgcgg ccacgtacga tgggcgccag caggtaaagg cgtgtgcctt cttccatttc 16501 catcacgcgg tcaaccattt gcgatacagt ctggctgaca atgggaaggc cggttgccgg 16561 ggaatagggg atgcccacac gcgcccaaag aaggcgcata taatcgtaaa tctcggtaac 16621 agtaccaacg gtcgagcgcg gattgcgcga tgtggttttc tgttcaatcg aaatggcggg 16681 cgacagaccg tcgatatatt cgacatccgg cttttgcatc atttcaagga actggcgcgc 16741 ataggccgac aggctttcga cataacggcg ctgaccttcg gcatagatgg tatcaaatgc 16801 cagcgaactt ttgcccgaac ccgacaggcc ggtaatcacc acaagggaat cgcggggcag 16861 ttcgacatca atgttctgca gattgtgttc tttcgcaccg cgaaccgaaa tttttgtcag 16921 catgtccggc cttttgttcc tgaattgttc gggcaatgta tagaggagcc agtgattata 16981 cgcaagtgcg cccctgacag ttcctgtcag gatttgtcag caggaaaaat gacaaagcct 17041 ggaaaggtag tggaggggaa ttggcgcaaa agaaaacccg ccagaatgac ctggcgggtt 17101 ttgcaaaatc aggcagggga ttaacccttg gcaatcgact tgccggtggc acccagatcc 17161 ttgaaagctt cttcaaggcg ggctgccatg gaaagttcgg cttcacgaac ccagacgcgc 17221 gggtcgtact gctttttgta cggtttgtcg tcttcgggat cgacctggta tttgaaggca 17281 cggtcgtttt cttcgacata tttgccaacg gctgtcgcat aggcaaattg ggtgtcagta 17341 tcgatattca ttttgaaaac gccgtagcca acagcttcct cgattttctc tttttccgag 17401 ccggaaccac cgtggaaaac gaaatcaagc gggaagccat caaggccatg tttatcagca 17461 acgtatttct gcgattccag aaggatttcc gggcgcagtt taacattgcc cggcttgtaa 17521 acgccgtgca cgttgccaaa tgccgccgca accgagaaat gccccagcgg tttcaggcgg 17581 cgataggctt cttcgacttc ttccggacgg gtataaaggc gcgggtcgat atcgtcctga 17641 gtggtatcga gttccttgcc aataccgtct tcttcgccac cggtaacgcc cagttcgatt 17701 tccaggctca tatcgacggc ggaaagacgt ttcagaaatt cctcgcaggt tgccagattt 17761 tcttcgagcg gttcttccga aagatcaagc atatgcgagc tgaacaaagg cttgccggtt 17821 tccttgtaag ctttttcacc ccagctcagc ataccatcga cccacggaac aaatttccgg 17881 ttggcatgat cggtatgcag aactacggcg acgccataat attcagccag aagctgggta 17941 tggcgggcgg cggaaacagc gccaataacg cgagcagcat tggcatcctt gatgcccttg 18001 ccggcaaaga actgtgcacc accattcgaa agctgaatga tgatgtcaga accggcattg 18061 gcagcagctt caagggcagc gttcagcgtg ctcgacgtgg taatattaac tgcgggtaga 18121 gcgtaaccgt cctgcttgca ggcagcaacg agttcacgat aggcagcacc ggttacaaca 18181 ccgggtttaa cggaaggcat gtgggtactc ctcctggggg tcagagcatt ataatattcc 18241 ccgtgcgaac cgattgatcc gcagcaaggg cgatccgtaa ggagcccaat gcatccgtca 18301 tatgctgcgt aaggtcaaga ttttccgtaa tagactttag gaaaaactgc tgttcacgca 18361 gacacaggcc atcatgatcg ggctcgtcat cggttctgat gatttcgtcc gaatgtgaaa 18421 attttccgtg cctatctgtg gcagcatgat gtaattttaa ggcattcgtc ttggtatgtg 18481 tgtcgatatc ggccgatccg gcgccttttt cttctacatc tgtgattgat accgaccctt 18541 tgggtccgat gacatcctta acgaaaaatg ccgtttcgct gatcatgggc ccccagcccg 18601 cttcgtacca gccaatcgac ccatcatcaa accggacctg caattgcccg taattataca 18661 tgtcctctgc aacatcgggc gacagacgca cccccattgc atgaaccgaa atcggctttg 18721 ccgctgtcat caggcacatc atatcaaggt aatgcacacc acaatcaaca atcggcgaca 18781 cgctgtttaa cagccgttta tgcgtttccc aaaattcgcc gctgctttgc tggttcaagt 18841 tcattcgcat caccagcggc ttgccaagtg tctttgccag ttcgacaaac ttttcccacg 18901 acgggtgatg gcgcaggata tagccgatca ccagtttacg gccatgcttc tgcgcacttt 18961 caatgacgcg ttcggcatcg accacggtgg cggcaagcgg cttttcgata aagacatgcg 19021 cacctgcttc aaaggcggca atggcgtaat ttgcatgggt ttctgtatag gtgttgatcg 19081 aaaccgcatc aggcccggtt tgggcaaggg cggcttcgta atcggtaaat tgcgggtagg 19141 attcaaatcc ttctggcagg gtgcccggtg cgatgggtga acgggtacat agcccaacaa 19201 tctcaaaccc ttccagggca tggtatgcct tggcatggga catccccata ttgcccagtc 19261 cgacgacaag gatgcgtacg ggtttcgacg tcattgggat gtctcctttg atgtaaggtt 19321 tcagataagg tgtttatctt aaatacctga aatccaggtg tgttttacct gaaatgtttg 19381 gggatcaaac aggaccatat cggcatcaaa acccggcatg attttgcctt ttctgtcatc 19441 aagtttcaaa aatgccgccg gataaagcga ggccatgcgc agcgcttcgc ccagatcaac 19501 gccaatcatc tcaacgctgt ttttcactgc cgaaatcata tcaaggtccg acccggcaag 19561 ggtgccgtcg gcaagtgcac aacggccatc aatggcaata atttcttcgc cgcccagaac 19621 aaaacgtttg gttttagcac ccaccgttgg catggcgtcg gtcaccagca tgatcttgcc 19681 tgtctgtttt gcgcgaacgg caacctgcat ggcggccgga tgcacatggt aaccatcggc 19741 aatcaggccg caccatgtgt ttttgtcagc tatggcagca cctgcaacgc cgggttcccg 19801 gtgggcaagg gggctcatgg cgttaaacaa gtgggtaaag ccacgcaaac cttcgccaat 19861 tgccttttgg atatcgtcat aggtcccggc agtatgcccg gcacaaacca gtacgccatt 19921 ggcgcacagt ttttcaattg tgcccggttt tgctttttcc ggggccatgg ttaccaaaat 19981 ccgcccattg ggcaggctgg acagaacctc aatcgcgtca tcttccatcg ggcggatgat 20041 gttggcgtca tgcacgccct tgcgttccgt gttcagatac ggaccttcaa ggtggatacc 20101 aacaatgccc ggcacgcctt cgtcgatggc gtttttaacc gcctcgatgg cggccagcat 20161 tttttcgcgt gtgtcggtga taagggtggg cagcatggcc gttgtgccaa actggcgatg 20221 ccccgccata atctggcgaa tgccatcaac gctgggcgtg tgatttagta aaacaccgcc 20281 gccgccatta acctgcacgt cgataaaccc cggtgccagc acatttcccc gggcgtcaaa 20341 aatgtcagcg ccatccggga tgcgggccgg tgacaccgca ctgactttac cgttttcaac 20401 gatcacgcag gcatcatcgt gaaactgctc gccatcgaac agccgggcat ttttaatggc 20461 aagaacagtc atcaatgcgt ctccgttacc ttggaaagat gcggcggtgc atcggggtta 20521 cagccgcgca gcaacgcaac atgttcagcc agggtataaa atgtctggat catcgcaatt 20581 ggcgctagca gcggatggat gtcttcgaca accggcaaat ggcccttggt cgcggcatca 20641 ccggtttcgg cggcaaacag gttgtcggtc tttgcgcgca tttcagtcag aaaaccctca 20701 acgctatcgc gggtggcgtc atcctgtgaa aagaccagca agggaaattc ccgtgttacc 20761 agggccatgg ggccgtgttt gacttcggcg gcactaaagg cttcggcatg caggctgctg 20821 gtttccttga atttaagggc agcttc //