LOCUS       NZ_PIZP01000014        12415 bp    DNA     linear   CON 30-MAR-2024
DEFINITION  Staphylococcus shinii strain INIFAP 005-08 NODE_15, whole genome
            shotgun sequence.
ACCESSION   NZ_PIZP01000014
VERSION     NZ_PIZP01000014.1
KEYWORDS    WGS; RefSeq.
SOURCE      Staphylococcus shinii
  ORGANISM  Staphylococcus shinii
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 28752)
  AUTHORS   Lopez,G. and Munoz,A.
  TITLE     Phylogenomics unveils the most efficient molecular markers for the
            species phylogeny - the case of the genus Staphylococcus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 28752)
  AUTHORS   Grana-Miraglia,L., Miranda-Mirand,E., Arreguin,C., Cossio-Bayugar,R.
            and Castillo-Ramirez,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-DEC-2017) CCG, UNAM, Av. Universidad, Cuernavaca,
            Morelos 62210, Mexico
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-FEB-2017
            Assembly Method                   :: Spades v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 337.1969x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002836835.1-RS_2024_03_29
            Annotation Date                   :: 03/29/2024 11:05:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,976
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 76
            rRNAs                             :: 7, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 22 of 41
            Pseudo Genes (incomplete)         :: 25 of 41
            Pseudo Genes (internal stop)      :: 13 of 41
            Pseudo Genes (multiple problems)  :: 13 of 41
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:11:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3255
            Orig. end                         :: 15670
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            PIZP01000014.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..12415
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..12415
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12415
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:7415]"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..7415
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            357..560
                     /locus_tag="CW747_RS15145"
     CDS             357..560
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CW747_RS15145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_232785956.1"
                     /transl_table=11
                     /translation="MKKKWMWLIEIVSVIGLVVVASVLKQVNSSSSKKDGYDTYTVEKE
                     NPLNLEGKASPNSIKTYNNNGL"
     gene            complement(669..791)
                     /locus_tag="CW747_RS15195"
     CDS             complement(669..791)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CW747_RS15195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_269801689.1"
                     /transl_table=11
                     /translation="MDDSESFPPLGSDSTGFEAEVSSHGPDIPAPALDLLNLSS"
     gene            802..1482
                     /locus_tag="CW747_RS14315"
                     /old_locus_tag="CW747_14310"
     CDS             802..1482
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017723558.1"
                     /locus_tag="CW747_RS14315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CW747_14310"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_101051432.1"
                     /transl_table=11
                     /translation="MIDDIDINRHFKNGDEQNHILKDINININEGEFIAIMGPSGSGKS
                     TLINILGFIDRVYEGDYLFNRKNYQESSDNQLADIRNKTVGFVFQNFKLIQNNTILENV
                     SIPIVYAGLGTQTRKSRVTQTLHDVGLFDKENLVPNKLSGGQQQRVAIARAIINQPKFI
                     IADEPTGALDSKTSKDIIELFIKLNEEHNTTMILVTHDRKVADQADRIIHILDGRVQRE
                     EVVG"
     gene            1479..2653
                     /locus_tag="CW747_RS14320"
                     /old_locus_tag="CW747_14315"
                     /pseudo=""
     CDS             1479..2653
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002451534.1"
                     /locus_tag="CW747_RS14320"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="CW747_14315"
                     /product="ABC transporter permease"
                     /pseudo=""
                     /transl_table=11
                     /translation="MKNLSNIIAISFKSILKISDVIFLR"
     gene            complement(2736..3365)
                     /locus_tag="CW747_RS14325"
                     /old_locus_tag="CW747_14320"
     CDS             complement(2736..3365)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024741.4"
                     /locus_tag="CW747_RS14325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CW747_14320"
                     /product="ABC-2 transporter permease"
                     /protein_id="WP_157822580.1"
                     /transl_table=11
                     /translation="MPGLLLSNLYATKTSIIAYSIIGIIGCIIFSFANPLFSGLLLIIS
                     LITPTIDNLKDEKSSNWVKYVSTLPISRDTYIKSQFVFYFLLSLLGSLLSIVIVFIITQ
                     NIISSINSGVLGLSLSFQYSLIYPLTFKIGENKSNMIQIISSLSVGILTFLFFYIIIII
                     TPVSSVSVLENLNHQIPFPFVFLFFSVCIIIISYFISVKILKYKDF"
     gene            complement(3347..4192)
                     /locus_tag="CW747_RS14330"
                     /old_locus_tag="CW747_14325"
     CDS             complement(3347..4192)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /locus_tag="CW747_RS14330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CW747_14325"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_101051414.1"
                     /transl_table=11
                     /translation="MTILKVQNLFKTYKKSNFDLTNITFEIQENQSIGLIGNNGSGKSS
                     LINILSGNSFKDSGIINFLDSINTKDKSQNEYLGVVFDDISLPKKINLKELNNIFKKVY
                     TTWNSQRFFELSNKLNLPLSQPISTFSKGMEMKTSIIVALSHHTRLLILDESTSGLDAT
                     SKKEINSILKEYVKKGNSILMTSHISNEIEELTTNLIFMKNGQIIFKEKTSTLLENYSI
                     LVMNEVEWQTYNKKNVEFFEKREDSIKILEKNEFSTEKLSIDTVLPLIMRGEILCQDYY
                     "
     gene            complement(4210..4866)
                     /locus_tag="CW747_RS14335"
                     /old_locus_tag="CW747_14330"
     CDS             complement(4210..4866)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /locus_tag="CW747_RS14335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CW747_14330"
                     /product="DsbA family protein"
                     /protein_id="WP_101051415.1"
                     /transl_table=11
                     /translation="MKKNLKIIIILSGILIVFLLTISFYFINKISFENVNGSKLEGDVI
                     QNPKHFSNSANHTIVAFGDFNCPYCKKYEENGYTKLKNNLIDSKKVNYYFVNAAFLGES
                     SIRASRAAHAVNIYAPKEYWKFHHLMYETQSSNKKNSLSNKIIDKNLSNLNLSKSILKK
                     IKKDYKTRKSTSWKLTERDQELTKKYNVKEVPTIYIDGKFVEDPYSFKSIEKELK"
     gene            5001..7151
                     /locus_tag="CW747_RS14340"
                     /old_locus_tag="CW747_14335"
     CDS             5001..7151
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002440725.1"
                     /locus_tag="CW747_RS14340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CW747_14335"
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="WP_101051416.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 4.4e-44, bitscore:
                     140.9, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 6.7e-186, bitscore:
                     611.0, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVKFIQQQDEKDCGPVCLAMISQYYGKRVSISKLRELSGTDTSG
                     TNMYGMIQAAKTIGLNMQAGKVPDLRELEQAPFPIIAHIQNKQGYLHYVVIEKMDKENV
                     FLVDPAIGRSKMKKKVFENYWTNIILIAKTNDNFTKNDDYPSIKPLFKNILKNNKFYII
                     IILTASVLINAIGFGGTYYFKILVDHILPTNILEHLNYLSLALLILYVTYSFSSYLRYH
                     LILELSLRIDFSLMKQYFAHVLKLPVHFFDTRKPGEILSRFLDISKIREAFSSVTVTLV
                     VDSLMVILGSILLYIQSPFLFYISLLFIPIYFILGFIFKGPFEQYNRKVMEENAFLSSY
                     LIETFSGASTIKNYNAEDEFYENGNKRFVQFLKQVRSLGVFKNIQLTLNDFFKLLANLT
                     ILWIGSMLVMEDQLTLGGLLTFNALLTYFLDPIERLINVQPIIQSSIVAARRIVEVTDL
                     KTENNKNDFYNTSNYSLEKIQTQNIYYRYNYDDYILKNINITINKGDLIGIVGESGSGK
                     STLAKLLCNYYSTKEGEILLNGEKIHKYSLNKLRSKIGYIDQNTFAFSKSIIENLTIGI
                     NKQVSIEKIVYYCKKVELHDFIMSLPNNYNTVLEDKGENLSGGQLQRLSIARALIKEPD
                     VYILDEATSALDSLNENKIMSYFRDLSNNKIVIIISHKLSNIRHSKTIYSLQNGCIVEN
                     GNHEQLMKNHSLYYQMWKNQNF"
     gene            complement(7242..7415)
                     /locus_tag="CW747_RS14345"
                     /old_locus_tag="CW747_14340"
     CDS             complement(7242..7415)
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF021923.4"
                     /locus_tag="CW747_RS14345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CW747_14340"
                     /product="Blp family class II bacteriocin"
                     /protein_id="WP_101051417.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 5.6e-12,
                     bitscore: 37.3, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFEALNEKELKSISGGLSKGAKCNIGTLGMAAGAFAAGGGPLGY
                     AAGGAVGMATFC"
     misc_feature    8030..8046
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: medium, score: 21.28"
                     /tool="antismash"
     gene            8249..8428
                     /locus_tag="CW747_RS14355"
                     /old_locus_tag="CW747_14350"
     CDS             8249..8428
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447495.1"
                     /locus_tag="CW747_RS14355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CW747_14350"
                     /product="hypothetical protein"
                     /protein_id="WP_101051419.1"
                     /transl_table=11
                     /translation="MKQKLTVFNIIAIIGIIIFIVTLFSSITLATKFIVFSMIFVFLVI
                     NAVIYYVLTRQTQV"
     gene            8540..8830
                     /locus_tag="CW747_RS14360"
                     /old_locus_tag="CW747_14355"
     CDS             8540..8830
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001044921.1"
                     /locus_tag="CW747_RS14360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CW747_14355"
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="WP_232785957.1"
                     /transl_table=11
                     /translation="MESNENKYVVIDVRNAPKEVKKDKIKSSIEIPAKDFKNQLSDLSK
                     EKIYVVYDWTGGTTLGKGELLELYKNNYEAYELAGALEGWKGMKLPLENIV"
     gene            9058..9609
                     /locus_tag="CW747_RS14365"
                     /old_locus_tag="CW747_14360"
     CDS             9058..9609
                     /GO_function="GO:0000150 - DNA strand exchange activity
                     [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467452.1"
                     /locus_tag="CW747_RS14365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CW747_14360"
                     /product="recombinase family protein"
                     /protein_id="WP_002508689.1"
                     /transl_table=11
                     /translation="MAKIGYARVSTQDQSLDGQIDTLEEYGCERIFSEKASGRKTNRTE
                     LDKCLDYLREGDILVIYKLDRLGRTTKQLIELSQWLDENSIDLHIIDMNVSTKDAMGKM
                     FFTMMSAFAELEANLLSERTKKGLEAARARGRKGGRPSLPDHKKREIKFLYDEQKLTGE
                     EIAKQTGVSRSTVYRIIKMK"
     gene            complement(9697..10734)
                     /locus_tag="CW747_RS14370"
                     /old_locus_tag="CW747_14365"
     CDS             complement(9697..10734)
                     /NRPS_PKS="Domain: PKS_ER (12-341). E-value: 2.1e-10.
                     Score: 32.1. Matches aSDomain:
                     nrpspksdomains_CW747_RS14370_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1040: alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019236158.1"
                     /locus_tag="CW747_RS14370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CW747_14365"
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="WP_002508690.1"
                     /sec_met_domain="ADH_N (E-value: 9.6e-26, bitscore: 81.2,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 2e-28, bitscore:
                     90.4, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKAAVWYGQKDVRVEDRKSKELQDNEVKVKVSWAGICGTDLHEYL
                     EGPIFISTDKPDPFLGQKAPVTLGHEFAGVVDDIGSKVTKFNKGDRVVINPTVSNREKE
                     ENIDLYDGYSFIGLGSDGGFAEFTNAPEENVYKLPDNVSDKEGALVEPTAVAVQAIKEG
                     EVLFGDTVAIFGAGPIGLLTTIAAKAAGASKIFVFDLSEERLNKAKSVGATHTINSGES
                     NPSDIISKYTDNGVDVAFEVAGVAPTLKSAIDVTKARGIVVIVSIFGHPIEWNPMQLTN
                     TGVKLTSTIAYTPTTFQQTIDLINEGNLKVKDVVTDEIELNDIVESGFEQLVNDKSQAK
                     ILVKL"
     aSDomain        complement(9712..10698)
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CW747_RS14370_PKS_ER.1"
                     /evalue="2.10E-10"
                     /label="CW747_RS14370_PKS_ER.1"
                     /locus_tag="CW747_RS14370"
                     /protein_end="341"
                     /protein_start="12"
                     /score="32.1"
                     /tool="antismash"
                     /translation="RVEDRKSKELQDNEVKVKVSWAGICGTDLHEYLEGPIFISTDKPD
                     PFLGQKAPVTLGHEFAGVVDDIGSKVTKFNKGDRVVINPTVSNREKEENIDLYDGYSFI
                     GLGSDGGFAEFTNAPEENVYKLPDNVSDKEGALVEPTAVAVQAIKEGEVLFGDTVAIFG
                     AGPIGLLTTIAAKAAGASKIFVFDLSEERLNKAKSVGATHTINSGESNPSDIISKYTDN
                     GVDVAFEVAGVAPTLKSAIDVTKARGIVVIVSIFGHPIEWNPMQLTNTGVKLTSTIAYT
                     PTTFQQTIDLINEGNLKVKDVVTDEIELNDIVESGFEQLVNDKSQAKI"
     gene            10914..11513
                     /locus_tag="CW747_RS14375"
                     /old_locus_tag="CW747_14370"
     CDS             10914..11513
                     /GO_function="GO:0000150 - DNA strand exchange activity
                     [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019169163.1"
                     /locus_tag="CW747_RS14375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CW747_14370"
                     /product="recombinase family protein"
                     /protein_id="WP_029379013.1"
                     /transl_table=11
                     /translation="MIVGYARVSTIDQNLDRQIENLQSFGAEKIFTEKQSGKSIENRPV
                     FQEALNFVRMGDRFVVESIDRLGRDYDEIIETVNYLKEKDVQLMITSLPMMNEVIGNPL
                     LDKFMKDLIIQILAMISEQERNESKRRQAQGIQVAKEKGIYKGRPALYSSNAKDPQKRL
                     VYYRVVELLEQGKSISTIAKEVGITRQTIYRIKNSR"
     gene            11574..11888
                     /gene="arsR"
                     /locus_tag="CW747_RS14380"
                     /old_locus_tag="CW747_14375"
     CDS             11574..11888
                     /codon_start=1
                     /gene="arsR"
                     /gene_functions="regulatory (smcogs) SMCOG1058: ArsR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_142257676.1"
                     /locus_tag="CW747_RS14380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CW747_14375"
                     /product="As(III)-sensing metalloregulatory transcriptional
                     repressor ArsR"
                     /protein_id="WP_029379012.1"
                     /transl_table=11
                     /translation="MSYKELSTILKVLSDPSRLEILDLLSCGELCACDLLEHFQFSQPT
                     LSHHMKSLVDNELVTTRKNGNKHMYQLNHEFLDYINQNLDIINTSDQRCACKNMKSGEC
                     "
ORIGIN
        1 ataacagctg ataatgtcat tactataatt gcgaggatta actttaatcc ccatttaggt
       61 ttttcacgta aatttgaaaa gtggtgaact aagggtaatt tagattcttc catttaattc
      121 tttcctccta tatgtatgat tcattaaagc atatcattcg tcatattgtt tttaaatatt
      181 tttattattt tcaatgtaaa ttgaaaaaca tttataaatt caatttgttg tatatgtgag
      241 agattgtact aagtatttct atattagcct gaaatataat actattttta ttatattttt
      301 aattgtgtag ctatgttagt cttggaaagt gaataaatat aaggagtgtt tatcaattga
      361 agaaaaaatg gatgtggctt atagagatag tatcggtaat tggattagtt gtcgtggcat
      421 cggttttaaa gcaagtcaat agtagttcgt cgaagaaaga tggttatgat acatatacag
      481 tggagaaaga aaatccactc aatttagagg gaaaagcatc acctaattca attaaaacat
      541 ataacaacaa cggtctctga atttgattta gataaaatca aagaaggaag caaagtagat
      601 gtcaaagtca caagtaacgg taagacaggt gaaggagaaa ttaaaaaagt ctctgaatta
      661 cctaaaagct atgaagataa gttaagtaaa tctagcgcag gtgctggtat atcaggtcca
      721 tgtgaagaaa cttcagcttc aaaccctgtt gaatccgatc cgagcggtgg aaatgattca
      781 gaatcatcca aatataccgt agtgattgac gatatagata tcaatcgaca tttcaaaaat
      841 ggggatgagc aaaaccatat tttgaaagac attaatatca acatcaatga aggtgaattc
      901 attgcgatta tgggtccttc aggttctggt aaaagtacgt taattaatat tttaggattt
      961 attgatcgcg tttatgaagg tgattacctt tttaatcgta aaaattatca agaaagttca
     1021 gataaccaac tggcagatat cagaaataaa acagttggtt ttgtctttca aaactttaaa
     1081 ttaattcaaa ataatacaat tttagaaaat gttagtattc caatcgttta tgcagggtta
     1141 ggcactcaaa cgcgtaaatc acgtgtaaca cagacgttac atgatgtagg tttgtttgat
     1201 aaagaaaatt tagtgcccaa caaattatcg ggtgggcaac aacaacgtgt tgccatcgca
     1261 cgtgccatta tcaatcaacc caagtttatt attgctgatg agcctacggg ggcacttgat
     1321 tcgaaaacat ctaaagatat tattgaatta tttataaaat tgaatgaaga acacaatacg
     1381 acgatgatat tagtcacgca tgatcgcaaa gttgcagatc aagcagatcg tattattcat
     1441 attcttgatg gccgagttca acgagaagag gtggttggat gaagaattta tctaatatca
     1501 tcgccatttc attcaaatct attttaaaaa taagcgacgt aatattttta cgatgattgg
     1561 tattattatt ggtattgcag ctgttattac aattatgtct ttaggaaacg gctttaaaaa
     1621 aacggcttct gaagaatttg aagatacagg tgctggaaaa aattctgcaa atattaattt
     1681 catgagtaat gatatggaag gtgtgaaaac gaaccctttc aaccaacaag acttgagtat
     1741 tataatacaa gttgacggcg tggcagatgc cgagattaaa gaaacagatg accaaggttt
     1801 ttctgcgaaa atgactaatg cgcagaaaaa agtcgatatc agtatagtga aaaaaggagc
     1861 tatggatagt catgaaaagg gacaaggctt tgatgaagat tataatgaat tacgaaagaa
     1921 agtcgtaata gttgatgata aaattgccaa agacgttttt aataaaaatg ccattggtaa
     1981 aacattatat attgatggtc aaggttttga agtcgtgggc attgccaaag acgtgatgaa
     2041 tccatcagct gtgaatatgc ctttaaaaac atttgagaat tatatgagtg gtttaagtca
     2101 agattttccg tcattaaaac tttcactgga agatggagct aataaaaaag atacagcaaa
     2161 taaggtcgta gataaattaa ataagaaagg ttctggaatc agtgatggta gctatattta
     2221 caatgacact gaagaaatga tgaaaggcat caatcaagtc tttgatgcga ttacttactt
     2281 tgtggcagca gtcgcaggta tatcattgtt tattgccggc attggtgtaa tgaatgtaat
     2341 gtatatttct gtttcagaac gtacagagga aattgcgatt cgacgtgcat ttggtgcgaa
     2401 gtcacgagat atcgaattac aattcttagt ggaaagcgtc gtgctttgtg tgattggtgg
     2461 tatcattggt ctgataatag gtatcttaat agcgtcaggc gtcgatgcgt taacgccaga
     2521 ctacattaag agtgttgtca gtctaggttc tgtgttaacg gcagttggtg tatctatgtt
     2581 aatcggcatc gtattcggtt ggatcccagc cagatccgca gctaagaaag aattgattga
     2641 tattataaaa tagcgtcata tatacatgtg aatataccat taaaaaataa aagaaatcaa
     2701 aacaacttct gtatatttac agaggttgtt ttgatttaaa aatctttata tttcagtatt
     2761 ttcacagaaa taaagtagga aataataata atacaaacac taaaaaataa aaatacaaag
     2821 gggaaaggaa tttgatgatt taaattttct aatacagata ctgatgaaac aggagtaatt
     2881 atgataatta tatagaaaaa taaaaaagtc aaaattccta cactaagtga ggagataatc
     2941 tgtatcatat tagatttatt ttcccctatt ttaaaggtta aaggatatat aagagagtat
     3001 tggaaactta aacttaaacc tagcactcca gaatttatag atgaaattat attttgagta
     3061 ataataaata ctattactat agacaaaagg ctacccaaca gagataacaa aaaataaaat
     3121 acaaattgtg attttatgta agtatcacga gaaatcggaa gtgttgatac atatttcacc
     3181 caattagaac tcttttcatc ttttaaattg tcaatagttg gggttataag agatattatt
     3241 agtaaaagac ctgagaataa tgggttagca aatgaaaaaa ttatacaacc aattattcct
     3301 attatagaat aagcgataat tgatgttttt gtagcatata aattacttag taataatcct
     3361 ggcacaatat ttcacctctc ataattaatg gtaaaactgt atcaatactt aatttttcag
     3421 tggagaactc atttttttct aaaattttta tagagtcttc tcttttttca aaaaattcca
     3481 catttttttt gttgtaagtt tgccattcga cttcattcat aactaaaata gagtaatttt
     3541 ctaatagagt tgatgttttt tctttgaaaa tgatttgccc atttttcata aatataagat
     3601 ttgttgtgag ttcttcgatt tcatttgaaa tatgtgaagt cattagaata ctatttcctt
     3661 tttttacata ctcttttaat atagagttta tttctttttt acttgtcgcg tctaaccctg
     3721 aggtagattc atctaaaatt aataaacgag tatgatggga taatgccaca attatcgaag
     3781 ttttcatttc cattcccttg gaaaatgtac ttattggctg agaaagtggt agattgagtt
     3841 tattagatag ttcaaagaac ctttgagaat tccaagtagt atagactttt ttgaaaatat
     3901 tgtttaattc ttttaaattt atttttttag gtaaacttat atcatcaaaa acaactccta
     3961 aatattcgtt ttgagattta tctttagtat ttatagagtc taaaaaatta ataattccac
     4021 tatctttaaa agaattaccc gacaatatat ttataagtga actttttcct gaaccattat
     4081 ttccaattag accaatactt tgattttctt gaatttcaaa agtaatgtta gtaagatcaa
     4141 agtttgattt tttgtacgtt ttaaataaat tttgcacctt taatattgtc atgctattaa
     4201 cctcaaatcc tattttaatt ctttttctat tgatttaaat gaatagggat cttctacaaa
     4261 tttcccatca atatatatag ttggaacttc ttttacatta tattttttgg ttaattcttg
     4321 atccctctca gttaacttcc atgaagtact ttttctagtt ttataatctt ttttaatttt
     4381 ttttaaaata gattttgata aatttaagtt acttaaattt ttatctatta ttttgttgct
     4441 cagactattc tttttattac ttgattgagt ctcgtacatt aaatggtgga acttccaata
     4501 ttctttaggg gcgtaaatat ttacagcatg tgcagctctg gaggcacgta tagagctttc
     4561 tcctaaaaaa gcagcattca caaaataata attaaccttt ttggaatcta ttaaattatt
     4621 ttttaattta gtatatccat tttcttcata tttcttgcag taaggacaat taaaatctcc
     4681 aaaagcaact atagtatgat tagcactatt actaaaatgt ttaggatttt gtataacatc
     4741 gccttccaat tttgatccat taacattttc aaatgagatt ttattaataa agtaaaaaga
     4801 tattgtgagt aaaaatacta ttaaaatacc gctaagtatt attatgattt ttagattttt
     4861 tttcatgatt acactctcca attaaatatt agaatatcat taaatccata caatataaat
     4921 aatgaattta gattgtatgg ataataataa cttttacgtg ttaaaatcaa aatgaaatta
     4981 ataagaaagg atctaaaata atgaaagtta aatttataca acaacaagat gaaaaagatt
     5041 gcgggccagt ttgtttggca atgatttctc aatattatgg caaaagagtc tctatatcta
     5101 agttaagaga attgtctgga acagatacta gtggaactaa tatgtatgga atgattcaag
     5161 ctgcaaaaac aatagggtta aatatgcaag cggggaaagt cccagactta agagagcttg
     5221 aacaagcacc atttcccatt atagctcaca tacaaaataa acaaggttat ttgcattatg
     5281 ttgttattga aaaaatggac aaggaaaatg tttttttagt tgatcctgca attggtagaa
     5341 gtaaaatgaa aaaaaaagtt tttgaaaatt attggactaa tataatttta atagctaaaa
     5401 ctaacgataa ttttacaaaa aacgatgatt acccaagtat aaaaccatta tttaaaaata
     5461 ttttaaaaaa caacaaattt tatataataa ttattttgac agcatctgta cttatcaatg
     5521 caataggatt tggaggcacc tattacttta aaatattagt agatcatatt cttcctacta
     5581 atattttaga acatttaaat tatctttcat tagcactttt aatactatac gttacatatt
     5641 ccttttcatc atatctaaga tatcacctaa ttttagaatt aagtttaaga attgattttt
     5701 cacttatgaa gcaatatttt gctcatgttc tcaaattacc tgtccacttt tttgatacta
     5761 gaaaacctgg tgaaattttg agtagatttt tagatatttc taaaattaga gaagcctttt
     5821 catctgtaac agtaacttta gtagtagatt cattgatggt aatcttaggt agtattttat
     5881 tgtatataca aagtcctttc ttattttata tatcactttt atttatacct atttacttta
     5941 tcttagggtt catatttaag gggccttttg aacaatataa tagaaaagta atggaagaaa
     6001 atgctttttt gagttcttat ttaatagaaa cattttctgg agcatctact ataaaaaatt
     6061 ataatgctga agatgagttt tatgaaaatg gaaataaaag atttgtgcaa tttctaaaac
     6121 aagtcaggag tttaggagta tttaaaaata tacaattaac tctcaatgat ttttttaaat
     6181 tattagctaa tctcacaatt ttatggatag gatctatgct tgtcatggaa gatcaattaa
     6241 cactaggagg gctactaaca tttaatgctt tgctcacata ttttttagat ccgatagaaa
     6301 gattaataaa tgtacagcct attatacaat catcgattgt tgctgctcga agaatcgtag
     6361 aagttactga tttgaaaact gagaataata aaaatgattt ctataataca agtaactatt
     6421 cactagaaaa aatacaaact caaaatattt attatagata taattatgac gactatattt
     6481 taaaaaatat aaatatcact attaataaag gggatctcat aggtatagta ggagaaagtg
     6541 gttcaggtaa atctacatta gctaagttat tatgcaatta ctattcaaca aaagaaggag
     6601 aaattctttt aaatggagaa aaaattcata agtattcatt aaataaattg agaagtaaaa
     6661 ttggttatat tgatcaaaat acttttgcgt ttagcaaatc aataattgaa aatctgacaa
     6721 taggtattaa caaacaagta agtatagaga aaattgttta ttattgtaaa aaggtagaat
     6781 tacacgactt tattatgagt ttgccgaata attataatac tgtattagaa gataaaggag
     6841 aaaacttatc tggaggacaa cttcagaggt tatcaattgc aagagctttg attaaagaac
     6901 cagatgtcta tattttagat gaagcaacta gtgctttaga ttcgttaaat gaaaataaaa
     6961 taatgagcta ctttagagat ttatctaata ataaaattgt cataataatt tctcataaat
     7021 tgagtaatat aagacattcg aaaacaatat attcattaca aaatggatgt atcgttgaaa
     7081 atggaaatca tgaacaacta atgaaaaatc attctttata ttatcaaatg tggaaaaatc
     7141 aaaattttta aaatcggaga tgttaataat aaaattgtca cgaattagag tctgggattt
     7201 atgtcccaaa ctcaatttaa agttttaaaa atactctttg tttagcagaa tgttgccatt
     7261 ccgacagctc cgcctgcagc gtatcctaaa ggtccaccgc ctgcagcaaa agcgccggct
     7321 gccattccta aagtacctat gttgcattta gcacctttgc ttaatccgcc tgaaatactt
     7381 tttaattctt tttcatttag tgcttcgaac ttcataaatc cacccactta atcatttttt
     7441 tattcctgaa aaaaattaat ttttttcagg aataagttat actcacaata ttataagttg
     7501 atataaaagt caaaccaata tatattgtat aacaaatgat acttgataaa gtgttcattg
     7561 attaatcttt ctatgtaatt taatattata ggaagctata aatattatgt agaggtgaga
     7621 atttgagatt tagtgaattg gttttatatc aaaaaattgc atttttaata ggaatgttag
     7681 gtgctctgtt attagcattt tgtcttattt caaattattt taatttaatt caaatcgcta
     7741 ttgatgctag cttaattaaa atattacgtt tagtaattgt attttcttta acagtattct
     7801 tttatagaat aaataagata tttactgtat ttatgtttat aatcctaatt aatgtatttt
     7861 tcttataact taagtgaaac atatagatta ctatttgaat ttgaaacgat taaacgactc
     7921 cttattcgat aaggagtcgt ttttatataa cttctgataa tcattatttt atttaaacta
     7981 ataaaaggaa gaatattatg taaacaggtc cactgaaatg tccaagctgt gatgaaaatt
     8041 tagggagcgt aagcaaagat agaatagact acatagttac aaaaaaataa tatagtttaa
     8101 gaatcttcat ttatggagca gcttcaatta atttattggg tggggttact tttagctatt
     8161 taatttcaac gaatcattag ttatgctagt aaagtaacaa tactgtatta aatacttatc
     8221 gagatcttag tcagaatgga ggattataat gaaacaaaaa ttgacagtat tcaatataat
     8281 agcaattata ggaattataa tatttatcgt tacattgttt tcaagtatta cactagcaac
     8341 taaatttatt gtgtttagta tgattttcgt ttttctagta attaatgctg taatatatta
     8401 tgtgctgaca aggcagactc aagtataata cattttcata catttatata aaatcaaatc
     8461 atgcttcttt attaaaaatc attattattg cgaaacattt ttaagtcttt atattagtca
     8521 ccatgaagta cttagagata tggaaagtaa tgaaaataaa tatgtagtaa ttgatgttag
     8581 aaacgcacct aaggaagtga aaaaggacaa aataaagagt tctattgaaa ttcctgctaa
     8641 agattttaag aatcaattga gtgatttatc taaagaaaaa atatatgtag tttacgattg
     8701 gactggagga actactttag gaaaaggaga attgttagag ttatacaaaa ataattatga
     8761 agcttatgaa ttagctggag cgttagaagg ttggaaaggt atgaaattac ctttagaaaa
     8821 cattgtatag tcattaaaac ctttcttttt taaccttcgt ttttgacaca aaacctctta
     8881 actataaatt aggaggtttt tcttggatat ttaagtctat aaacttgtgc tataatcaat
     8941 gtatcataaa tatcaaaaat catgagtttt tagacgatga gagacgcgtt tatttgcatc
     9001 gagaattaga tttcgttaat aagtacccat aaaacgatta aaagaggtgg tattcaaatg
     9061 gctaaaattg gttatgcacg tgtatcaaca caagatcaaa gtcttgatgg acagattgat
     9121 acacttgagg aatatggttg tgaacgtatc tttagcgaga aagcgagtgg ccgtaaaacg
     9181 aatagaacag aacttgataa atgcttagat tatttacgtg agggagatat tctagtgatt
     9241 tataaactag atcgtcttgg acgtacaacg aaacaattaa ttgagttatc acaatggtta
     9301 gatgaaaaca gcattgactt acatattata gatatgaacg tatcaacgaa agatgcgatg
     9361 ggcaagatgt tctttaccat gatgagtgct tttgcggaac ttgaagctaa cctactaagt
     9421 gaacgtacga aaaaaggact agaagctgcg agagcaagag gacgaaaagg tggacgccct
     9481 tcattgcccg accataagaa gagagagatt aaattcttat acgatgaaca aaaacttact
     9541 ggagaagaaa ttgctaaaca aacaggagtg agtcgttcta ctgtatatag aataataaaa
     9601 atgaagtagt aaaaacaaat aatcacagga agaaaactaa tgaaaggaga atctctaatt
     9661 tgaagattct cctttcatta gtttttataa cctatattat aatttaacga gaatttttgc
     9721 ttgtgattta tcgtttacaa gttgttcaaa tcctgactct acaatatcat ttaactcaat
     9781 ttcatcagta acgacgtctt ttacttttaa attaccttcg ttaattaagt ctattgtttg
     9841 ttgaaatgta gtaggtgtat aagcaatcgt cgaagtaagt tttacacctg tattcgttaa
     9901 ttgcattgga ttccattcaa taggatggcc gaatatagaa actataacaa caatacctct
     9961 tgctttagta acatctattg cagatttaag tgttggcgct acaccagcta cctcaaatgc
    10021 tacatcaacg ccattatcag tgtatttact aataatatca cttgggtttg attcaccaga
    10081 atttatcgta tgagtagcac ctactgattt agctttatta agtcgttctt ccgataaatc
    10141 gaaaacaaat attttgctag caccagctgc tttggctgct atagttgtta ataaaccgat
    10201 tggccctgca ccaaaaatag caacagtatc gccaaataga acttctcctt ctttaattgc
    10261 ttgaactgca acagccgtcg gttctacaag ggcgccttct ttatcagaaa cattatctgg
    10321 taatttataa acattctctt ctggggcgtt tgtaaattct gcaaatccac catcagaacc
    10381 taagcctata aatgaataac catcataaag atcaatattt tcttcttttt cacgatttga
    10441 aactgtagga ttaatgacaa cacgatcgcc tttattaaat tttgtaacct tagaaccaat
    10501 atcatctact acacctgcaa attcgtggcc taatgtaacg ggcgcttttt ggccaaggaa
    10561 cgggtcaggt ttgtctgttg aaataaatat agggccttct aagtattcat gtaaatctgt
    10621 accacaaatg ccagcccaag acactttaac tttaacctca ttatcttgta actctttaga
    10681 ttttcgatct tcaactcgta catccttttg accataccaa actgctgctt tcattgataa
    10741 aacctccttg ttttagttaa tttaatagta tgccttttaa aagttacttt caaataaact
    10801 aaccttttaa tatagtgttt ctaaattatt atatgattag agtgtaccct aatcgaacaa
    10861 taaaaaaccc ttgtaaaatg ctgtttctgt ttgtatgaga aagggtgtta aaaatgatag
    10921 ttggatatgc aagagtttcg accattgatc aaaatttaga taggcaaatt gaaaatttgc
    10981 aatcttttgg tgctgagaaa atttttacag aaaaacaatc aggtaaatct atagaaaaca
    11041 gacccgtctt tcaagaagct cttaattttg tgagaatggg agatcgattc gttgtggaat
    11101 ctattgatcg tttgggacgt gattatgatg aaattattga aactgtgaat tacttaaaag
    11161 aaaaagacgt tcagttaatg attacaagtt tacctatgat gaatgaagtg attggaaatc
    11221 cacttctaga taaattcatg aaagacttaa ttatccaaat tttagcaatg atttcagaac
    11281 aagaaagaaa tgaaagtaaa cgtagacaag ctcaaggtat tcaagtcgca aaagaaaaag
    11341 gtatatataa aggaagacct gctctgtatt cttccaatgc taaagatcca caaaaacgtt
    11401 tggtttatta ccgagttgtt gaattacttg aacaaggtaa atctataagt actatagcta
    11461 aagaagtagg tattacacgc caaactatat atagaattaa aaacagtaga taaaagagaa
    11521 tagacgcaaa tctatataga tgttaatcta ttaactgtct aggaggtaca ttcatgtctt
    11581 ataaagaact atcaacaata ttaaaagttt tatcagatcc aagtaggttg gaaatactgg
    11641 atttactatc ttgtggggag ttatgtgctt gtgacttgct agagcatttt caattttcac
    11701 aacctacgtt aagccatcat atgaagtcgt tagtagataa cgagttagtt actacacgaa
    11761 aaaatggtaa taaacatatg tatcaactta atcatgaatt tttagattat attaatcaaa
    11821 acttagatat cattaataca tctgaccaac gctgtgcttg taaaaatatg aagtcaggtg
    11881 agtgttaatg acaattttag caattgtgat ttttctttta actttaatct ttgtaatatg
    11941 gcaaccgaaa ggtttagata ttggtattac agctttaatt ggagctgtcg ttgctatcat
    12001 tacgggagtc gtaagttttt ccgatgtatt agaagtaaca ggtattgttt ggaatgctac
    12061 cttaacattt gtagctgtta ttcttatttc attaatatta gatgaaattg gattttttga
    12121 atggtctgcg atacatatgg tcaaggcttc aaacggtaat ggattaaaaa tgtttgtttt
    12181 tatcatgtta cttggggcaa ttgtagcagc atttttcgca aatgatggtg cagctttaat
    12241 cttaacgcct attgtattag caatggtaag aaatctgggt tttaataaaa aagtgatttt
    12301 tccttttatt attgctagtg gttttattgc tgatactaca tcactacctt taattgtaag
    12361 taacttagtt aatatcgttt ctgcagatta cttcgatatt ggatttattg aatat
//
