LOCUS       NZ_CP025018            10405 bp    DNA     linear   CON 12-FEB-2024
DEFINITION  Streptomyces sp. M56 chromosome.
ACCESSION   NZ_CP025018
VERSION     NZ_CP025018.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. M56
  ORGANISM  Streptomyces sp. M56
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 11742376)
  AUTHORS   Kim,K.K.Hyun., Klassen,J.L., Ruzzini,A.A.C., Beemelmanns,C.C. and
            Clardy,J.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-NOV-2017) Leibniz Institute for Natural Product
            Research and Infection Biology - Hans Knoll Institute,
            Adolf-Reichwein-Strasse 23, Jena 07745, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-AUG-2014
            Assembly Method                   :: HGAP v. AUG-2014
            Assembly Name                     :: M56v2
            Expected Final Version            :: yes
            Genome Coverage                   :: 94.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/12/2024 06:37:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,558
            CDSs (total)                      :: 9,473
            Genes (coding)                    :: 9,167
            CDSs (with protein)               :: 9,167
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 86 of 306
            Pseudo Genes (incomplete)         :: 261 of 306
            Pseudo Genes (internal stop)      :: 28 of 306
            Pseudo Genes (multiple problems)  :: 61 of 306
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:58:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 8288586
            Orig. end                         :: 8298991
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP025018.1.
            Annotated using prokka 1.11 from http://www.vicbioinformatics.com.
            Bacteria and source DNA available from Christine Beemelmanns.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="32"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5405)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(221..991)
                     /locus_tag="CFP59_RS34425"
                     /old_locus_tag="CFP59_06813"
     CDS             complement(221..991)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1195: IclR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381154.1"
                     /locus_tag="CFP59_RS34425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_06813"
                     /product="IclR family transcriptional regulator"
                     /protein_id="WP_069869920.1"
                     /transl_table=11
                     /translation="MSAAETGGAQVKSAVRTVELLEFFAGRPGMHSLAAVQEAVGYPKS
                     SLYMLLRTLVELGWVETDATGTRYGIGVRALLVGTSYIDGDEVVAAARPTLDRLSDDTT
                     ETIHLARLDGTNIVYLATRQSQHYLRPFTRVGRRLPAHSTSLGKALLATYSDEQVRKLL
                     PETLSQLTEHTITDREKLIEELHLVREQGYAVDREENTLGLRCFGIAIPYRTPSRDAIS
                     CSVPVARLTPAHEQMIKDALFDARDRLTLATRRL"
     gene            1079..2662
                     /locus_tag="CFP59_RS34430"
                     /old_locus_tag="CFP59_06814"
     CDS             1079..2662
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014059810.1"
                     /locus_tag="CFP59_RS34430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_06814"
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="WP_100807593.1"
                     /sec_met_domain="Aldedh (E-value: 2e-42, bitscore: 144.4,
                     seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MCETQAAVTPQQEESAVSAPVWSVDPRTGKQREQVAVEATAEEVD
                     RVVRAAHAARGSLADRAVRAALLRTAADLLDEARDQLVEAADAETALGPARLTGELART
                     TYQLRSFATIVDEGGFLDVRIDHADATQTPPWPDLRRYKLPLGVVAVYAASNFPLAFSV
                     PGGDTASALAAGCPVIVKAHPDHPATSEVAASALRRAAERVGVPAEVIAVVHGFDAGIE
                     LVRHPLIAAAGFTGSVRGGRALHDAAASRPVPIPFHGELGSLNPVVVTEAAAADRGDQI
                     GAGLAGSMTLGVGQFCTKPGFVLAPAGPAGDGLVKSLTEAVSNTEAGVLLDHRMRDNFV
                     AGVQERAELADVEAPVTPGAGGEHTVSAGFLTVPAGRLAESGPHDALLEECFGPVTVVA
                     RYESDEEISAVLGRLPGNLTATVHLSAAEAEGTEGRAGELIAELTPLAGRVLVNGWPTG
                     VAVAPAQHHGGPYPATTSTSTSVGGTAIERWLRPVAYQDTPAALLPPELRDENPLELPR
                     RINGVREQRG"
     gene            2928..3974
                     /locus_tag="CFP59_RS34435"
                     /old_locus_tag="CFP59_06815"
     CDS             2928..3974
                     /NRPS_PKS="Domain: Aminotran_5 (14-342). E-value: 2.9e-25.
                     Score: 80.9. Matches aSDomain:
                     nrpspksdomains_CFP59_RS34435_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014180697.1"
                     /locus_tag="CFP59_RS34435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_06815"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_100807594.1"
                     /sec_met_domain="Aminotran_5 (E-value: 9.3e-17, bitscore:
                     60.1, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MESLAGAEFAPHTTYLTTASSGLIPARSVAAVQTVLDGSATGQPI
                     VDVAFEAVEAARAAYARLVGVPARRVAAGSSVAVYAGMIATSLPPGAEVLVAEGDFSSL
                     VNPFAVRGDLKLRIVPLEEIAEAVRPGTALVAVSAVQSADGRIADLDAIREAAGAHGAR
                     TLVDVSQAAGWFPVNAAEADYTVCVAYKWLMCPRGAAFLTVPEDLGGLTPVFAGWVAGE
                     KPWDSCYGPVEELAHSARRFDESPSVYSYVAARHSLALIEELGTEAIAAHDRALADRFR
                     DGVIGLGHRPIAAPGSAIVSVPGLGDAAPRLAEAEVHVSARAGNLRASFHLYNTEADVD
                     RLLEKLSG"
     aSDomain        2970..3953
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CFP59_RS34435_Aminotran_5.1"
                     /evalue="2.90E-25"
                     /label="CFP59_RS34435_Aminotran_5.1"
                     /locus_tag="CFP59_RS34435"
                     /protein_end="342"
                     /protein_start="14"
                     /score="80.9"
                     /tool="antismash"
                     /translation="YLTTASSGLIPARSVAAVQTVLDGSATGQPIVDVAFEAVEAARAA
                     YARLVGVPARRVAAGSSVAVYAGMIATSLPPGAEVLVAEGDFSSLVNPFAVRGDLKLRI
                     VPLEEIAEAVRPGTALVAVSAVQSADGRIADLDAIREAAGAHGARTLVDVSQAAGWFPV
                     NAAEADYTVCVAYKWLMCPRGAAFLTVPEDLGGLTPVFAGWVAGEKPWDSCYGPVEELA
                     HSARRFDESPSVYSYVAARHSLALIEELGTEAIAAHDRALADRFRDGVIGLGHRPIAAP
                     GSAIVSVPGLGDAAPRLAEAEVHVSARAGNLRASFHLYNTEADVDRL"
     gene            complement(4109..4996)
                     /gene="thpD"
                     /locus_tag="CFP59_RS34440"
                     /old_locus_tag="CFP59_06816"
     CDS             complement(4109..4996)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009718912.1"
                     /locus_tag="CFP59_RS34440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_06816"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_069869917.1"
                     /sec_met_domain="PhyH (E-value: 1.7e-39, bitscore: 135.3,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAVTDLYPTRGAVEVATPRVDPVVWSAPGAPGPLQPSELSDFDR
                     DGFLAIDELITPDEVAEYRAELDRMIADPDVRADERSIIEPTSQDVRSVFEVHRISEVF
                     AKLVRDPRVVGRARHILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPNMRT
                     VSVSVALTENFAHNGSLMIMPGSHRTFLGCAGATPKDNYKKSLQMQDAGTPSDEALSGF
                     ADKHGIRQFTGKPGSAVWFDCNCMHGSGDNITPFSRSNVFIVFNSVENTAVEPFAAPVP
                     RPDFIGARDFTPVR"
     gene            complement(5001..5405)
                     /locus_tag="CFP59_RS34445"
                     /old_locus_tag="CFP59_06817"
     CDS             complement(5001..5405)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009718913.1"
                     /locus_tag="CFP59_RS34445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_06817"
                     /product="ectoine synthase"
                     /protein_id="WP_069869916.1"
                     /sec_met_domain="ectoine_synt (E-value: 3.8e-53, bitscore:
                     177.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEDTERHVRSKSGTWESKRIVLAKEGVGFSLHETILY
                     AGTETSMWYANHIEAVLCVEGEAELTNDETGEKHWITPGTMYLLDGHEKHTMRPKTDFR
                     CVCVFNPPITGREDHDENGVYPLITETEEA"
     gene            complement(5446..6708)
                     /gene="ectB"
                     /locus_tag="CFP59_RS34450"
                     /old_locus_tag="CFP59_06818"
     CDS             complement(5446..6708)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-363). E-value: 2.7e-72.
                     Score: 235.6. Matches aSDomain:
                     nrpspksdomains_CFP59_RS34450_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="CFP59_RS34450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_06818"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_093700080.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.4e-75, bitscore:
                     253.6, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFEALESEVRSYCRGWPAVFDRAQGSHMYDEDGHTY
                     LDFFAGAGSLNYGHNNPVLKRALIDYIERDGVTHGLDMSTTAKRAFLESFQNHILRPRD
                     LPYKVMFPGPTGTNAVEAALKLARKVKGREAIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGTIPDFLWFERLLEDQGSGLNRPAAVIVETVQGEGGINVARP
                     EWLRALAELCERQDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTVSKSISGYGMPMS
                     LALFKPELDIWEPGEHNGTFRGNNPAFVTAAATLDTYWADGQMEKQTLARGEQVELALR
                     AICEEQSGTQFRGRGLVWGLEFDDKPRATAVCRRAFELGLLLETSGPESEVVKLLPALT
                     ISPDDLDEGLRTLARAVRETA"
     aSDomain        complement(5620..6624)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CFP59_RS34450_Aminotran_3.1"
                     /evalue="2.70E-72"
                     /label="CFP59_RS34450_Aminotran_3.1"
                     /locus_tag="CFP59_RS34450"
                     /protein_end="363"
                     /protein_start="28"
                     /score="235.6"
                     /tool="antismash"
                     /translation="FDRAQGSHMYDEDGHTYLDFFAGAGSLNYGHNNPVLKRALIDYIE
                     RDGVTHGLDMSTTAKRAFLESFQNHILRPRDLPYKVMFPGPTGTNAVEAALKLARKVKG
                     REAIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGTIPDFLWFER
                     LLEDQGSGLNRPAAVIVETVQGEGGINVARPEWLRALAELCERQDMLLIVDDIQMGCGR
                     TGAFFSFEEAGITPDIVTVSKSISGYGMPMSLALFKPELDIWEPGEHNGTFRGNNPAFV
                     TAAATLDTYWADGQMEKQTLARGEQVELALRAICEEQSGTQFRGRGLVWGLEFD"
     gene            complement(6779..7339)
                     /gene="ectA"
                     /locus_tag="CFP59_RS34455"
                     /old_locus_tag="CFP59_06819"
     CDS             complement(6779..7339)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014059805.1"
                     /locus_tag="CFP59_RS34455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_06819"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_100807595.1"
                     /transl_table=11
                     /translation="MTAAQADLVRSELDLPEDLTLDTPRVEDGAAIWRIARDSKALDLN
                     SSYSYLLWCRDFAATSVVARDADGGPVGFITGYVRPERPETLVVWQVAVDHAQRGRGLA
                     ATLLDGLVKRVAATGVRGIETTITPDNTASNRLFTSFAERHQAPLEREVLFHGGLFPDG
                     AHEPEVLYRIGPLGSLGPSTDRT"
     misc_feature    complement(7364..7376)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 18.12"
                     /tool="antismash"
     misc_feature    7497..7512
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.52"
                     /tool="antismash"
     misc_feature    complement(7561..7576)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 21.4"
                     /tool="antismash"
     misc_feature    complement(7572..7585)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.61"
                     /tool="antismash"
     gene            complement(7660..8472)
                     /locus_tag="CFP59_RS34460"
                     /old_locus_tag="CFP59_06820"
     CDS             complement(7660..8472)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014059804.1"
                     /locus_tag="CFP59_RS34460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_06820"
                     /product="hypothetical protein"
                     /protein_id="WP_100807596.1"
                     /transl_table=11
                     /translation="MIEHRERAEGRDVVAELRAAAASHEPDRSRMLARVTAGIAADDRR
                     RNRAPRMGPRRSRMVGPVAGLAAAVAATGLVVLATDGAERPQTVRTSSEPVVPPAGAGA
                     DTRPGEEPGAGSQSEAERHTPSAYPTAAGPNHLAEPTVRSKGAINAHSNPYWAQSDITL
                     TIGRPLTSLTVELRVAETGGVRSTGSWSSLPTSDMTAATHIEDGALVYRWTLRKGATVP
                     AGRFVFAGQYNHAEGERDNGRDAYSASGNGDSGAFAVRGDFPRFTVGD"
     gene            complement(8472..9059)
                     /locus_tag="CFP59_RS34465"
                     /old_locus_tag="CFP59_06821"
     CDS             complement(8472..9059)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009718917.1"
                     /locus_tag="CFP59_RS34465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_06821"
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="WP_231981821.1"
                     /transl_table=11
                     /translation="MARLAHLLLGSSDGADDLAADALVAVWHQWDRVRAADHPAAYARG
                     VVANLARSRIRSLVRERRRVALFSSQRSDHVDDPDVSAVVDVQAALVRLPFRKRACVVL
                     RHAFDLSERETARTLGISVGTVKSQTSRGIAELERLLGPPDGEGELPGATDAGRTVRTL
                     RSGNTGPAGTGPTGPARHSGQMGHAGHAGGGS"
     misc_feature    9517..9530
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.11"
                     /tool="antismash"
ORIGIN
        1 cggccttgag gccgatgtag gggagacagg cgaggatcgc gacggcccgc aggatccgtg
       61 cggtcaggtc accgggcagg cgggcgggtg cggagcgcgt ccgggggccg gggagggtgt
      121 gggtcatggt caccagcctc ccggcgcggt gggcgcggcg cctcaccccg cccgacgatc
      181 cccctccgcc gcatggcgga ggcggccgtc ctcccgtggg tcagaggcgg cgcgtcgcga
      241 gggtgagccg gtcccgggcg tcgaacaggg cgtccttgat catctgctca tgggcggggg
      301 tgagccgggc caccggcacc gagcagctga tggcgtcgcg cgagggggtg cggtacggga
      361 tggcgatgcc gaagcagcgc aggcccaggg tgttctcctc gcggtccacc gcgtacccct
      421 gctcccggac gaggtgcagc tcctcgatca gcttctcgcg gtcggtgatg gtgtgctcgg
      481 tgagctggct cagcgtctcc ggcagcagct tgcgcacctg ctcgtcggag tacgtggcca
      541 gcagcgcctt gcccagcgag gtggagtggg cgggcagccg gcgcccgacg cgggtgaagg
      601 ggcgcagata gtgctgcgac tgccgggtgg cgagatagac gatattggtg ccgtcgagcc
      661 gggcgaggtg gatggtctcg gtggtgtcgt ccgagagccg gtccagggtg gggcgggccg
      721 cggccacgac ctcgtcaccg tcgatgtagg aggtgccgac gagcagggcg cgcaccccga
      781 tgccgtagcg ggtgccggtg gcgtcggtct ccacccagcc gagctccacc agggtgcgca
      841 gcagcatgta gaggctcgac ttgggatagc ccacggcctc ctgcaccgcg gcgaggctgt
      901 gcataccggg ccgcccggcg aagaactcca gcagttcgac ggtgcggacc gccgacttga
      961 cctgggcccc acccgtctcg gcagctgaca tcgccttgac ccctctgttc gaccagccat
     1021 agagtcccag ggggaaattc atcacccggg acagcgttca gcatatcgaa caccgtcgat
     1081 gtgtgagacg caagcagcgg taacaccaca gcaggaggaa tccgcggtgt cagcaccagt
     1141 gtggagcgtc gacccccgaa ccggaaagca gcgcgagcag gtcgcggtcg aagccacggc
     1201 ggaggaggtc gaccgcgtgg tgcgcgccgc ccatgccgcc cgcggttccc tggccgaccg
     1261 cgcggtgcgc gccgccctgc tgcgcacggc cgccgacctg ctcgacgagg cccgtgacca
     1321 gctggtcgag gccgccgacg cggagaccgc gctcggcccc gcccggctca cgggcgaact
     1381 ggcccgcacc acctaccagc tgcggtcctt cgcgaccatc gtggacgagg gcggcttcct
     1441 cgacgtccgc atcgaccacg ccgacgccac ccagaccccg ccctggcccg atctgcgccg
     1501 ctacaagctg ccgctcggcg tcgtcgccgt ctacgccgcc agcaacttcc cgctcgcctt
     1561 ctccgtgccc ggcggcgaca ccgcgagcgc cctcgccgcg ggctgcccgg tcatcgtcaa
     1621 ggcccaccct gaccaccccg ccacctccga ggtggccgcc tccgcgctgc gccgcgccgc
     1681 cgagcgggtc ggggtgcccg ccgaggtgat cgcggtggtg cacggcttcg acgcgggcat
     1741 cgagctggtc aggcatccgc tgatcgccgc cgccggtttc accggatcgg tccgcggcgg
     1801 ccgcgccctg cacgacgcgg cggcctcccg gccggtgccg atccccttcc acggcgagct
     1861 cggcagcctc aacccggtgg tggtcaccga ggccgcggcg gccgatcgcg gcgaccagat
     1921 cggcgcgggg ctcgcgggct ccatgacgct gggtgtcggc cagttctgca ccaagcccgg
     1981 cttcgtcctg gccccggccg gcccggcggg cgacggcctg gtgaagtccc tcaccgaggc
     2041 ggtcagcaac accgaggccg gagtcctgct cgaccaccga atgcgggaca acttcgtcgc
     2101 cggggtccag gagcgcgccg agctggcgga cgtggaggcc ccggtcaccc ccggcgccgg
     2161 cggtgagcac acggtcagcg cgggcttcct gaccgtcccg gcggggcggc tggccgaaag
     2221 cggcccgcac gacgcgctgc tggaggagtg cttcggcccg gtgaccgtcg tggcgcgcta
     2281 cgagagcgac gaggagatca gcgcggtgct cggtcggctc ccgggcaacc tcaccgccac
     2341 cgtccacctc tccgctgccg aggccgaggg caccgagggc cgggccggtg agctgatcgc
     2401 cgagctcacc ccgctggctg gccgggtcct ggtcaacggc tggccgaccg gtgtcgcggt
     2461 cgcccccgcc cagcaccacg gggggcccta cccggctacc acctccacct ccacctcggt
     2521 cggcggcacc gccatcgagc gctggctgcg gccggtcgcc taccaggaca ccccggccgc
     2581 cctgctgccg ccggagctgc gcgacgagaa cccgctggag ctgccgcggc ggatcaacgg
     2641 cgtccgggag cagcgcggct gacgcccgga acgacggcac ggccgccgga cccgcggacc
     2701 tgacccgcgt gtccggcggc cgtttcggca tgcccgcggc cgcgtacgtc gtacggggcc
     2761 tgaggcggtc ggcctgatgc ggccgatccg cggggtgtgt tcacccgggt gccgtgccac
     2821 gtggaggaat cgccgcaggt cagcggggat gcataaggat cgcttgaagg catcccacaa
     2881 gaaatcgcga tggacgccgg ggtgagcgcg ttccatagtg gttggacatg gagagcctgg
     2941 cgggcgccga attcgcgcca cacacgacgt atctcaccac cgcgtccagc ggtctgatcc
     3001 ccgcacgctc cgtagccgcc gtgcagacgg tcctcgacgg ctcggccacc ggacagccca
     3061 tcgtcgacgt ggccttcgag gcggtcgagg cggcccgtgc cgcctacgcc cggctggtcg
     3121 gcgtaccggc ccggcgggtc gcggcgggca gctccgtcgc cgtgtacgcg gggatgatcg
     3181 ccacctcgct gccgcccggc gccgaagtcc tggtggccga gggcgacttc agctccctgg
     3241 tcaacccctt cgccgtccgc ggcgacctca agctgcgcat cgtcccgctg gaggagatcg
     3301 cggaggcggt acggcccggt acggcgctgg tggccgtgag cgcggtccag tcggcggacg
     3361 ggcggatcgc cgatctcgac gccatccggg aggcggcagg ggcccacggg gcccgcaccc
     3421 tggtggacgt cagccaggcg gccggctggt tcccggtgaa cgccgccgag gccgactaca
     3481 ccgtctgcgt ggcctacaag tggctgatgt gcccgcgcgg cgccgccttc ctgaccgtgc
     3541 cggaggacct cggcggactc acccccgtct tcgcgggctg ggtcgcgggg gagaagccct
     3601 gggacagctg ctacggcccg gtggaggaac tcgcccactc cgcccgccgc ttcgacgaga
     3661 gcccgagcgt ctactcctac gtggcggccc ggcactccct ggcgctgatc gaggagctgg
     3721 gcaccgaggc catcgccgcc catgaccgcg cgctcgccga ccgcttccgg gacggggtca
     3781 tcggactcgg ccaccggccc atcgccgccc ccggctcggc catcgtctcc gtacccggcc
     3841 tgggcgacgc cgcgccccgg ctggcggagg cggaggtcca tgtctcggcg cgggccggta
     3901 acctccgcgc ctccttccac ctctacaaca ccgaggccga tgtggaccgg ctgctggaga
     3961 agctgtccgg ctgagtcggc tccccgcggc caaagcctga cgcgccaagc aggcggatgg
     4021 tgcaaaggga cagggcaaaa atctgaggga agccggggca ctccggcttc cctcagatca
     4081 cggacgtcac atcagcgcat ggcggttgtc accgtacggg cgtgaagtcc ctcgcaccga
     4141 tgaagtccgg ccgggggacc ggcgcggcga acggctcgac cgcggtgttc tccacgctgt
     4201 tgaacacgat gaacacattg ctgcgggaga acggcgtgat gttgtcgccg gacccgtgca
     4261 tgcagttgca gtcgaaccag accgccgagc cgggcttgcc ggtgaactgt ctgatgccgt
     4321 gcttgtcggc gaagccgctg agcgcctcgt cggacggggt gcccgcgtcc tgcatctgaa
     4381 gcgacttctt gtagttgtcc ttcggcgtcg cgcccgcaca gcccaggaag gtgcggtgcg
     4441 agcccggcat gatcatcaag ctgccgttgt gcgcgaagtt ctcggtgagc gcgaccgaga
     4501 ccgacacggt ccgcatgttc ggcagcccgt cctcggcgtg ccaggtctcg aagtccgagt
     4561 gccagtagaa gcccgaggcg ccaaagcccg gcttgacatt gatccggctc tggtggacgt
     4621 agacgtccga gccgaggatg tgccgagcgc ggcccaccac acgcgggtcg cgcaccagtt
     4681 tggcgaagac ctcgctgatc cggtgcacct cgaacaccga ccgtacgtcc tgcgacgtcg
     4741 gctcgatgat cgagcgttcg tcggcgcgca cgtccggatc ggcgatcatc cggtccaatt
     4801 cggcgcggta ttccgccacc tcatccggag tgatcagctc atcgatcgcc aggaagccgt
     4861 cgcgatcgaa atcgctcagc tcggacggtt gcagcggccc tggggctccg ggcgcggacc
     4921 acaccacggg gtccacgcgc ggtgtcgcca cctccacagc cccacgggtc gggtacaggt
     4981 cagtgaccgc ggtcatcggt tcatgcctcc tcggtctcgg tgatcagcgg gtatacgccg
     5041 ttctcatcat ggtcctcccg tccggtgatc ggcgggttga atacacagac gcaccggaaa
     5101 tccgttttgg ggcgcatcgt gtgcttctca tgtccgtcga gcagatacat ggtgccgggc
     5161 gtgatccagt gcttctcacc ggtctcgtcg ttggtgagct cggcctcgcc ctcgacgcac
     5221 agcaccgcct cgatgtggtt ggcgtaccac atcgacgtct cggtgcccgc gtagaggatg
     5281 gtctcgtgca acgagaaccc gacgccctcc ttggcgagca cgatgcgctt gctctcccag
     5341 gtgccggact tggacctcac atgcctctcg gtgtcctcga tgtccttgaa ggatcggacg
     5401 atcacggtga gttcttgcct ttctcttctc tgcggtgcgg gaggatcatg cggtctcgcg
     5461 gaccgcgcgg gcgagggtcc gcagcccctc gtccaggtca tcgggggaga tcgtcagcgc
     5521 gggcagcagc ttgacgacct cgctctcggg gccggacgtc tccagcagca gccccagctc
     5581 gaaggcgcgc cggcagaccg ccgtggcgcg cggcttgtcg tcgaactcca gcccccacac
     5641 caggccacgg ccgcggaact gggtgccgga ctgctcctcg cagatggcgc gcagggccag
     5701 ctcgacctgc tcgccgcggg ccagggtctg cttctccatc tggccgtcgg cccagtaggt
     5761 gtccagggtc gcggcggcgg tcacgaacgc ggggttgttg ccgcggaagg tgccgttgtg
     5821 ctcaccgggc tcccagatgt ccagctcggg cttgaagagg gcgagcgaca tcggcatgcc
     5881 gtagccgctg atggacttgg agacggtcac gatgtccggg gtgatgcctg cctcctcgaa
     5941 ggagaagaag gcgcccgtgc ggccgcagcc catctggatg tcgtcgacga tcagcagcat
     6001 gtcctggcgc tcgcacagct cggccagggc gcgcagccac tcgggccgcg ccacgttgat
     6061 gccgccctcg ccctgtacgg tctcgacgat cacggcggcg ggccggttga gaccggagcc
     6121 ctggtcctcc agcagccgct cgaaccacag gaagtccggg atcgtgccgt cgaagtagtt
     6181 gtcgaacggc atcggggtgc cgtggaccag cgggatgccc gcaccggcgc gcttgaaggc
     6241 gttgccggtc acggccagtg agcccagcga catgccgtgg aaggcgttgg tgaaggagac
     6301 gatggcctcg cggcccttga ccttacgggc cagcttcagg gcggcctcga cggcgttggt
     6361 gccggtcggg cccgggaaca tgaccttgta gggcagatcg cgcgggcgca ggatgtggtt
     6421 ctggaacgat tcgaggaacg cgcgcttggc cgtggtggac atgtccaggc cgtgcgtgac
     6481 gccgtcacgc tcgatgtagt cgatcagcgc ccgtttcagc accgggttgt tgtggccgta
     6541 gttgagcgac ccggctccgg cgaagaagtc gaggtacgtg tggccgtcct cgtcgtacat
     6601 gtggctgccc tgcgcgcggt cgaagacggc gggccagcca cggcagtagc tgcgcacctc
     6661 cgactccagg gcctcgaaga cgctcagggc gggcggggtg atggtcacag cgttctcctg
     6721 ggagaggggt gtgagggggg tgaggagggt gagggttcga gggggatcga ggtgggggtc
     6781 aggtccggtc ggtgctcggc ccgagggagc cgagcggacc gatgcggtag agcacctcgg
     6841 gctcgtgcgc accgtcgggg aacagtccgc cgtggaagag cacctcacgc tcgagcggcg
     6901 cttgatgccg ctcggcgaac gaggtgaaca gccggttcga ggcggtgttg tccggggtga
     6961 tcgtcgtctc gatgccgcgg acgcccgtcg cggcgacccg cttgaccagc ccgtccagca
     7021 gggtggcggc cagtccgcgg ccacgctggg cgtggtcgac ggcgacctgc cagacgacca
     7081 gggtctcggg gcgctcgggg cggacgtagc cggtgatgaa gccgaccggt ccgccgtcgg
     7141 cgtcgcgcgc cactacggat gtggcggcga agtcgcggca ccacaggagg tagctgtagg
     7201 aggagttgag gtccagggct ttggagtcgc gtgcgattcg ccagatcgcg gctccgtcct
     7261 cgacgcgcgg ggtgtcgagg gtcaggtcct ccggcaaatc caattcgcta cggacaaggt
     7321 ctgcttgtgc ggcggtcatg caaattcaat ttacccagca gaatcggaaa atgcatcgcg
     7381 gtggggggtt acgcgaaagg gggtggtcgt gttatcacgc gggagcgcgc gtgcgcgcgg
     7441 ccgcacgccg aaagaggctg atttgtgggg gagataccgg ggcaaagtga atctcatgtg
     7501 tggtccgtca catgtgcgta atcgcccggt gactgtcttg aattacggga tcaggatccc
     7561 gtgaaaaaat tttgtgttta ggaatcacaa aagcgggcag acgaatgtgg aagcagtagc
     7621 ggaaaaacgc ggagaatttc tggccaagct gccatgaatt caatctccca cggtgaaccg
     7681 ggggaagtcg ccccgcaccg cgaaggcccc ggagtcaccg ttgcccgagg ccgagtaggc
     7741 gtcccggccg ttgtcccgct cgccctcggc gtggttgtac tgccccgcga agacgaaccg
     7801 gcccgcgggt acggtcgcac ccttccggag cgtccagcgg tagaccagcg caccgtcctc
     7861 gatatgcgtg gcggcggtca tatcggaggt cggcagcgag ctccaggagc cggtggaccg
     7921 cacacccccg gtctccgcga cccgcagctc caccgtcagc gaggtcagcg gcctgccgat
     7981 ggtgagcgtg atgtcgctct gcgcccagta agggttggaa tgggcgttga tggcgccctt
     8041 ggaccgtacg gtcggctcgg cgaggtggtt tggaccggcc gcggtgggat aggcgctcgg
     8101 cgtgtgccgc tcagcttcgc tctgcgaccc ggcgcccggc tcctccccgg gccgtgtgtc
     8161 cgcgcccgcg cccgccgggg gcacgacggg ttcgctcgag gtgcgcaccg tctgcggccg
     8221 ctcggcaccg tccgtggcca gcaccacgag gcccgtcgcg gcgaccgccg ccgccagccc
     8281 ggcgaccggg cccaccatcc gggaccggcg cgggcccatc cgcggggccc ggttccggcg
     8341 tcggtcatcg gccgcgatgc ccgccgtgac cctcgccagc atcctgctcc ggtccggttc
     8401 atgggaggcg gcggccgcgc gcagctcggc gacgacatcg cggccctcgg cgcgctcgcg
     8461 atgctcgatc atcaactgcc ccctcctgcg tgcccggcgt ggcccatctg acccgagtga
     8521 cgagcggggc ccgtgggacc tgtgcccgcg ggacctgtgt tcccggagcg gagcgtccgt
     8581 accgtccgcc ccgcgtccgt cgccccgggc agctcgccct cgccgtccgg agggccgagc
     8641 agccgctcca gctcggcgat tccgcgggag gtctgactct tgaccgtgcc caccgaaatg
     8701 cccagcgtac gggccgtctc ccgctcggag aggtcgaacg cgtgccgcag caccacacat
     8761 gcccgcttgc ggaacggcag ccggaccagc gcggcctgca catccaccac ggcggacaca
     8821 tcggggtcgt ccacatggtc ggagcgctgc gaggagaaga gcgccacccg gcgccgctcg
     8881 cgcaccaggc tgcggatccg ggagcgggcc agattggcga ccacgccccg ggcgtacgcg
     8941 gccggatggt cggcggcccg cacccggtcc cactggtgcc agaccgccac cagcgcgtcg
     9001 gccgccaggt cgtccgcccc gtcggagctg ccgagcagca gatgggcgag gcgggccagc
     9061 tcggtgtggt gccgctcgaa gaagtcccgg aactcggctt gtgggtcggg tgggccgggt
     9121 gggtcggggt gcggggcggc gctctgtaag tcggcgtgcg gtgcggccgg tgggtcgggg
     9181 tgcccagtgg ccggggagcc ggccggtaag tcggggtggg cgtcggccga taagccgtcg
     9241 gggccctccg gcgcgtcggg cgcctccggt gctgcggccc gtggtgggtc cgtcgtgtgc
     9301 gtcgtgtcca tcggtacggc tcgcgtggtg ctcgcggcgg ccgcgtcatc ggtcggagcg
     9361 ctcacgagga cctcctcact gccgctcctc cggtgggttg cgtgagcgta gcagcgctgc
     9421 gcaagttgtt cgagtggcgc cgttccggct cctgggaatt ggccggtcaa cgccctgccc
     9481 cggtctcgta cagtgcgttg catgagggat agcgctgtgc tccatatcaa gggccgggtc
     9541 ctggtcggac cggacaccgg cgacgctgcc ggggacatcc gggacgagct gtgggtggtg
     9601 gacggccgga tcacgttcga gcggccggcc ggggccaggg acattcggct gatcgagggc
     9661 tgggtgctgc ccggactcgt cgacgcccac tgccatgtcg gcctcgacgc ccatggcgcg
     9721 gtggacgact ccaccagcga gaaacaggcg ctgaccgacc gtgacgccgg ggcgctgctg
     9781 ctgcgggacg cgggatcgcc ggccgacacc cgctggatcg acgaccgcga ggatctgccc
     9841 cgcatcatcc gggccggccg ccatatcgcc cgtaccaggc gctacatccg caactacgcg
     9901 tatgagatcg agcccgagga cctggtcgcc tatgtggccg cggaggcgcg gcgcggcgac
     9961 ggctgggtca agctggtcgg cgactggatc gaccgggaga ccggtgacct ggggtcctgc
    10021 tggccgcgcg gcgcggtcga ggcggccatc gcggaggcgc accggctggg cgcgcgggtc
    10081 acggcgcact gtttcgccga ggagagcctg gccccgctgg tcgaggcggg gatcgactgc
    10141 atcgagcacg ccaccgggct caccgaggag accatcccgc tgttcgccga gcggggcgtg
    10201 gcgatcgtcc cgaccctggt caacatcgcc accttcccgc ggctggcgct gggcggcgag
    10261 gcgaggttcc cgcgctgggc cgaccatatg cggcggctgc acgagcgccg gtacgagacg
    10321 gtgcgggcgg cctacgacgc gggcatcccg atctacaccg gcaccgacgc gggcggcggc
    10381 ctggcccatg ggctggtcgg gcagg
//
