LOCUS       NZ_CP025018            11572 bp    DNA     linear   CON 12-FEB-2024
DEFINITION  Streptomyces sp. M56 chromosome.
ACCESSION   NZ_CP025018
VERSION     NZ_CP025018.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. M56
  ORGANISM  Streptomyces sp. M56
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 11742376)
  AUTHORS   Kim,K.K.Hyun., Klassen,J.L., Ruzzini,A.A.C., Beemelmanns,C.C. and
            Clardy,J.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-NOV-2017) Leibniz Institute for Natural Product
            Research and Infection Biology - Hans Knoll Institute,
            Adolf-Reichwein-Strasse 23, Jena 07745, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-AUG-2014
            Assembly Method                   :: HGAP v. AUG-2014
            Assembly Name                     :: M56v2
            Expected Final Version            :: yes
            Genome Coverage                   :: 94.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/12/2024 06:37:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,558
            CDSs (total)                      :: 9,473
            Genes (coding)                    :: 9,167
            CDSs (with protein)               :: 9,167
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 86 of 306
            Pseudo Genes (incomplete)         :: 261 of 306
            Pseudo Genes (internal stop)      :: 28 of 306
            Pseudo Genes (multiple problems)  :: 61 of 306
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:58:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3421521
            Orig. end                         :: 3433093
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP025018.1.
            Annotated using prokka 1.11 from http://www.vicbioinformatics.com.
            Bacteria and source DNA available from Christine Beemelmanns.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11572
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="20"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11572
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11572
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6572](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6572
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    336..349
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: medium, score: 20.04"
                     /tool="antismash"
     misc_feature    430..445
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.88"
                     /tool="antismash"
     misc_feature    1110..1125
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 21.93"
                     /tool="antismash"
     gene            1267..2526
                     /locus_tag="CFP59_RS13725"
                     /old_locus_tag="CFP59_02697"
     CDS             1267..2526
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055544.1"
                     /locus_tag="CFP59_RS13725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_02697"
                     /product="hypothetical protein"
                     /protein_id="WP_231981250.1"
                     /transl_table=11
                     /translation="MAAPIGALTAVAVVVGLLIALTSPGDAGGGTSDGNLPAGAASPKS
                     DRVPREPKKEDKYADWDGEVKVLGDGSTSYTGPQPHQPKPEKLKPGEKPPQFVVFSWDG
                     ALENDDHLFSRFRQVAKESDATMTFFLSGMYLLPKTKSDLYQPPQHKRGSAAISFPTDE
                     HIRETLEQLRGAWLEGNEIGSHFNGHFCGAKGGQDWSTQEWKSETEQAYSFVKRWKTNT
                     GFTDIEPLPFDYDVELVGGRAPCLEGQKTLIPAIKSLGWRYDASSPGDFQLWPSKIDGI
                     WNFPLQLVPYPESEKQVLSMDFNFLFNQSGDSTEGDPAKYAEWERLTRQGYLNGFERAY
                     NGSRAPLFIGNHFETWNGGIYMQAIEDVMRSVCRRDGVRCVSFKQLADWLDAQDPKVLE
                     KLRMLDPAESPDWSTYTKTR"
     gene            complement(2557..4536)
                     /locus_tag="CFP59_RS13730"
                     /old_locus_tag="CFP59_02698"
     CDS             complement(2557..4536)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055543.1"
                     /locus_tag="CFP59_RS13730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_02698"
                     /product="transglycosylase domain-containing protein"
                     /protein_id="WP_100805898.1"
                     /transl_table=11
                     /translation="MRLRRWAAARIRLDYPRTGRRGVGRWLPSWRQIALFFLFCVGSLT
                     GLLSFLYLQTDIPKNLNDFATQQDNVYYWADGTEMARTGPVNRQEVPLKKVPEQVQWAI
                     LAAENASFYSDRGVSPSGLMRAVTRMVTGGETQGGSTITQQYVKNAYLNQRQTFSRKLT
                     EMFMAIKLDDRMSKRDILERYLNTSWFGRGSYGIQRASYAYYGKDVSELNASEGALLAS
                     LLKGAGTFDPTLGAKNHKRAVDRWKWVLDRMVDIGRLSKSERARYTKFPEPKAPPKPAG
                     LTGQVGYLVETAKAYVSSHTEISDADFDLGGYQIHTTFEKPKVNALAKAVEQTRDKLDP
                     ERRPEDRHVRVGAASVAPGGAIRALYGGPGYLEQGFNDANASNVPAGTSFTPFVYAAAL
                     RDGVLLQRNGARTPVTPSTLYDGNNKIAVHTPEGPYWDRGGKIVRATNDGDASYGQVSL
                     RQAIVQSINTPMIQLGMDVGLDRVRRVSIDAGLLPSSFGARVPAFSLGTATPSSIRMAS
                     AYGTFAAEGTHHAPYSVSGLTHGGQKVELRKDRARRAFSAEVAAQVDDALRGAVQSPDG
                     SARGAAMADAEVAGKGGTAQDGTSAWFVGYTKELSTAVSLSRLDPKSQELMPLEGMGGG
                     GADTSGDTYPLDIWKSYMAGRTAE"
     misc_feature    complement(4725..4739)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.81"
                     /tool="antismash"
     misc_feature    complement(4726..4739)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.6"
                     /tool="antismash"
     misc_feature    complement(4727..4747)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 20.73"
                     /tool="antismash"
     gene            5001..6572
                     /locus_tag="CFP59_RS13735"
                     /old_locus_tag="CFP59_02699"
     CDS             5001..6572
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010983658.1"
                     /locus_tag="CFP59_RS13735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_02699"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_100805899.1"
                     /sec_met_domain="DUF692 (E-value: 8.2e-93, bitscore: 309.6,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVRLGTGIGWRPEIADDIARLPGIDWVEVVAENICPDHVPDALRL
                     LRERGTTVVPHGVSLGLGGAERPEPGRLRALAERAEALGSPLVTEHIAFVRAGGPLTAS
                     PSIEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLAVENIAALISWPGEEMTEGQFLA
                     ELVERTGVRLLIDVANLHTNHVNRGEDPAKALDELPTSAIAYVHVAGGVEKDGVWHDSH
                     AHPVTQPVLDVLAELCARTAPPGVLLERDEDFPAAGELAGELAAIRAVTAAGAAGAAEA
                     GMATAGTAAGSAKPVTAAGAAEAATAARAAEAATAARTGDEAPAPVRASAAADEADKAD
                     EADKARTGADVPAADAPAADAPAADAPAPETPLPDAPLPEAPVPDAVRERLALAQTALL
                     SALVGGTPPPEGFDRRRLRVQSAALTAKRASVIAKVAPELPEILGTGYRPAFARYAEGR
                     PMTGGYRRDALSFAEHLLADGRPDDSAARRRLTLWWQERSGPAPLPSHPAARFARRVRL
                     ALSGGR"
     gene            6578..7588
                     /locus_tag="CFP59_RS13740"
                     /old_locus_tag="CFP59_02700"
     CDS             6578..7588
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009714418.1"
                     /locus_tag="CFP59_RS13740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_02700"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_100809163.1"
                     /transl_table=11
                     /translation="MGTIASLVYAVVTVSSAILITGTVRARRRRVSGPVHAQAYDLLEA
                     AFLAGGPGRVADTVISTMYADGRLAIGGPGVVSVRQPVARGPVEEELLRLSANSPHGAL
                     TWLRRELMRSAAVQEIGDRLAGRGLMVRPDALRFWRRMARTHITLCAVGAFLAIMISIG
                     PVTYDGPPLIFMIAPALFIGFLIGRICAGAGKRRLTAAGKRALVAYRRESGVSGRHARA
                     RSIGRPATVSVAVVVALTGAATLTDDPQLRAQLLEAQKVPSGSGSPGYSGSSGDSGFSA
                     SDGGGSWCGGDGGGGGSSCGGSSCGGSSGGGCGGGSGCGGGGGCGGGGGGGCGGG"
     gene            8452..9258
                     /locus_tag="CFP59_RS13745"
                     /old_locus_tag="CFP59_02701"
     CDS             8452..9258
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055540.1"
                     /locus_tag="CFP59_RS13745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_02701"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_100805900.1"
                     /transl_table=11
                     /translation="MLWVLFLLVAWGAAIVSCTRLCLAAVAAAQPMEPDPAADGKGLSL
                     YEAAFLSGGPRRVGELALVTMCRERRLLLAHTGWVTVVDPEGRDELERSVITAIGPEGQ
                     ALVRPVRAAFATAAPVRALGDRLVAAGLAVPASARTNVAVAVRQVRAACALVLAMAVAA
                     LLLVPPAAGRGPVASWFMLPLLLTVSCLLIARVEAHPYSRWASPAGQRLLGRIDVPPRR
                     TVAQRSGGGPDGGADDADVLTALAVHGTVALSDRALRAALKTHWGH"
     gene            9344..11185
                     /locus_tag="CFP59_RS13750"
                     /old_locus_tag="CFP59_02702"
     CDS             9344..11185
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (170-557). E-value:
                     1.5e-20. Score: 65.8. Matches aSDomain:
                     nrpspksdomains_CFP59_RS13750_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055539.1"
                     /locus_tag="CFP59_RS13750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_02702"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_231981251.1"
                     /sec_met_domain="PF00561 (E-value: 2e-23, bitscore: 82.4,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAAALYGILGPLSLSLTMSALLATPATGRPLTAPTTPAATAATP
                     STRAATPPAGAAGAPLTAAPAARSAAPADPVAATAPVWTTWSGSSAAAAAPPADPAAAR
                     GVALAGRRAAERGVTFRRCPRAESLAAPITCGKVSVPLDYARPRGKRVSLTVSRVRASG
                     PKARRQGALIYNPGGPGASGMAFPEYAAEKSFRRLAAVYDFVGYAPRGVGRSAPLSCQR
                     PSAYAKAPTSSPPHPTAAFKRRLVARARAYVRGCVRRSGRALAHYTTLNNARDLEVLRA
                     ALGERRLTFVGASYGTYYGAVYATLFPGRVRRMVFDSVVNPAPWQIWYRDNLDQSLAFE
                     RRWRDWRRWVARHHRVYHLGATADRVLASYEKARRRLARKPAGGVVGTAQLQSAFLRTG
                     YNDAYWDPAARALAAYLLGDSKPLIALAAPDRAQAADDENGNAVYTAVECNDAPWPREW
                     ATWDRDNTALARRAPFETWDNVAMNLPCAFWPVKPGRPLDVGNLEAVGPRTGRVALPPV
                     LLLSAERDAATPYAGAKELWHRLPGSSLVTERKAGTHGLWGGPNGCVNRHVDTYLLTGR
                     TPGRSAYCAPRPAPEPQPEPAPLPDSGKQPAALPGTP"
     aSDomain        9854..11014
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CFP59_RS13750_Abhydrolase_1.1"
                     /evalue="1.50E-20"
                     /label="CFP59_RS13750_Abhydrolase_1.1"
                     /locus_tag="CFP59_RS13750"
                     /protein_end="557"
                     /protein_start="170"
                     /score="65.8"
                     /tool="antismash"
                     /translation="ALIYNPGGPGASGMAFPEYAAEKSFRRLAAVYDFVGYAPRGVGRS
                     APLSCQRPSAYAKAPTSSPPHPTAAFKRRLVARARAYVRGCVRRSGRALAHYTTLNNAR
                     DLEVLRAALGERRLTFVGASYGTYYGAVYATLFPGRVRRMVFDSVVNPAPWQIWYRDNL
                     DQSLAFERRWRDWRRWVARHHRVYHLGATADRVLASYEKARRRLARKPAGGVVGTAQLQ
                     SAFLRTGYNDAYWDPAARALAAYLLGDSKPLIALAAPDRAQAADDENGNAVYTAVECND
                     APWPREWATWDRDNTALARRAPFETWDNVAMNLPCAFWPVKPGRPLDVGNLEAVGPRTG
                     RVALPPVLLLSAERDAATPYAGAKELWHRLPGSSLVTERKAGTHGLW"
ORIGIN
        1 gcgggctgct gggagccgtc tgggcggcgg ccatggcctg gatctggtcg ctggcgctgg
       61 tcgacggctg gcagcggggg gtggcggaac ggctgacggc ccgccatgaa tatctgcgcg
      121 gcgtcgatcg gttccacgac atcggcgcgg ccctgcgcgg cttcaccggc catatcctgc
      181 tcactcagcc cgaccactgg cccgcccata tcgcgggcca tccgccgggc gccgtgctca
      241 ccttcgtggg cctggaccgg ctgggcctgg gcggcggcgg ttgggccggg gcattctgcg
      301 tcaccgtggg cggctcggcg gcggccgcgg tgctggtcac gctgcgcgcc ctggggcggg
      361 agcgggccgc gcgcgcggcg gccccgttcc tggtcctggc gccgggggcg gtgtgggtgg
      421 gcgcctcggc ggacggctat ttcacggccg tcgcggcgtg ggggctcgcg ctgctggcgc
      481 tcgcggcgac gcgtacggtc cgggcgcccg gggcggtcgc gttcggcgcc ggactgctgc
      541 tggggctgac ggtgtatctc tcctacgggc tgacgctgct ggccctgccc gccgccgtcg
      601 tgctgctgct cgcccgcacc gcgcggccgc tgcccttcgc gctgaccggg gtggcggtgg
      661 tggccggtgc gttcaccctg gtcggcttcc agtggtggga ggggtactcc ctgctggtcg
      721 agcgctacta ccagggcgtg gccgccgacc gcccgtacgg gtactgggtc tgggccaatc
      781 cggccgtcgc gacggttgcc gccggtccgg cctgtgtcgc gggtgtgcgg cgcatgctgt
      841 cggcggtgcc ggatgccgtc cggcgactgc ggccgggcgg accgggcgcc ccgggcggcg
      901 tcccggacgg gctcgtcgtg ctggccttcg ccgcgctgct cgcgctcgtc gtggccgatc
      961 tgtcagggat gagcaaggcc gagacggagc ggatctggct tcccttcatc ctctggctgc
     1021 tgcccaccac cgcgctgctg cccgccgttg accgccaccg atggcttctg gcgcaggcgt
     1081 gtctcgcact gctcgtcaac cacctgttgt tgaccggttg gtgaggagac tgatgtgaag
     1141 agcacgtgat acttctgtga tactgaggga gacgtacatc tgtactgttg ccagggggcg
     1201 ggacccgtga aggcgctgaa gatcgtgaaa gcgttgcagg ctttaagcgc gaggaacgcc
     1261 tatggcgtgg ccgcgcccat aggcgccctg acggccgtgg ccgtcgtggt ggggctgctg
     1321 atagccctga cctcgccggg ggacgcgggc ggcggcacgt cggacgggaa cctccccgcc
     1381 ggtgccgcgt cccccaagtc cgaccgggtc cccagagagc ccaagaagga agacaagtac
     1441 gccgattggg acggcgaggt gaaggtgctc ggggacggct ccacgtccta caccggaccg
     1501 cagccgcacc agcccaagcc cgagaagctc aaacccggtg agaaaccgcc gcagttcgtg
     1561 gtcttctcct gggacggcgc gctggagaac gacgaccacc tcttctcccg cttccgccag
     1621 gtcgccaagg agagcgacgc cacgatgacg ttcttcctca gcggtatgta tctgctgccc
     1681 aagacgaaga gcgacctcta tcagccgccc cagcacaagc ggggctcggc cgccatctcc
     1741 ttccccaccg acgagcacat cagggagacc ctggagcagc tgcgcggtgc ctggctggag
     1801 ggcaacgaga tcggctccca cttcaacgga cacttctgcg gcgccaaggg cgggcaggac
     1861 tggtccaccc aggagtggaa gagcgaaacg gagcaggcgt actcctttgt gaagcggtgg
     1921 aagaccaaca ccggcttcac cgacatcgaa ccgctgccgt tcgactacga cgtggagctg
     1981 gtgggcgggc gcgcaccgtg cctggagggc cagaagacgc tgatcccggc gatcaagtcc
     2041 ctcggctggc gctatgacgc gagctcaccc ggcgacttcc agctctggcc gtccaagatc
     2101 gacggcatct ggaacttccc gctccagctg gtgccgtacc ccgagagcga gaagcaggtc
     2161 ctctcgatgg acttcaactt cctcttcaac cagtccggtg acagcaccga gggcgatccg
     2221 gcgaagtacg ccgagtggga gcggctgacc cgccagggct atctgaacgg cttcgagcgc
     2281 gcctacaacg gcagccgggc gccgctgttc atcggcaatc acttcgagac ctggaacggc
     2341 ggcatctata tgcaggccat cgaggacgtg atgaggagcg tctgccgccg cgacggagtg
     2401 cgatgcgtgt cgttcaagca gctcgccgac tggctggacg cccaggaccc gaaggtcctg
     2461 gagaagctgc ggatgctgga cccggcggag tcgcccgact ggtcgacgta caccaagacc
     2521 aggtaagcgg tcccgttgcc gggcttccgc cgtcgctcat tccgccgtac ggcccgccat
     2581 atagctcttc cagatgtcca gggggtaggt gtcacccgaa gtgtcggcgc ctcctccgcc
     2641 catcccctcg agcggcatca gctcctgcga cttggggtcc agccgggaca gcgagaccgc
     2701 cgtcgagagc tccttggtgt agccgacgaa ccaggcggag gtgccgtcct gcgccgtgcc
     2761 gcccttaccg gccacctcgg catcggccat ggccgccccg cgcgccgagc cgtccgggct
     2821 ctggaccgcg ccgcgcagcg cgtcgtccac ctgtgccgcg acctccgcgc tgaaggcccg
     2881 tctggcgcgg tccttacgga gctcgacctt ctgcccgccg tgggtgagcc cgctcaccga
     2941 gtacggggcg tggtgcgtgc cctcggcggc gaacgtcccg taggcgctgg ccatacggat
     3001 cgagctgggc gtcgcggtgc cgagcgagaa cgcgggcacc cgcgcgccga agctgctggg
     3061 cagcagcccc gcgtcgatgg acacccgccg gacccggtcc aggccgacgt ccatgcccag
     3121 ctggatcatc ggggtgttga tggactgcac gatcgcctgg cgcagcgaga cctgcccata
     3181 ggaggcgtcg ccgtcgttgg tggcccgcac gatcttgccg ccgcggtccc agtacggccc
     3241 ctcgggtgta tgcaccgcga tcttgttgtt gccgtcgtag agcgtcgagg gcgtcacggg
     3301 cgtccgtgcc ccgttgcgct ggagcagtac gccgtcgcgc agcgcggcgg cgtagacgaa
     3361 gggggtgaag gaggtgccgg ccgggacgtt ggaggcgttc gcgtcgttga atccctgctc
     3421 gagatagccg gggccgccgt acagcgcgcg tatcgcgccg ccgggggcca ccgaggcggc
     3481 gcccacccgc acatgccggt cctcggggcg ccgctcggga tcgagcttgt cccgggtctg
     3541 ctccaccgcc ttggcgagcg cgttgacctt gggcttctcg aaggtcgtgt ggatctgata
     3601 gccgcccagg tcgaaatcgg cgtcggatat ctcggtatgg gagctcacat acgccttggc
     3661 cgtctccacc agatagccga cctgaccggt gagcccggcc ggcttggggg gcgccttggg
     3721 ctcggggaac ttcgtatagc gtgcccgctc ggacttcgac agccttccga tgtcgaccat
     3781 ccggtccagc acccacttcc agcggtcgac ggcccgcttg tggttcttgg cgccgagcgt
     3841 cggatcgaat gtcccggcgc ccttgagcag cgaggcgagc aacgcgccct cgctcgcgtt
     3901 gagttccgag acgtccttgc cgtagtacgc gtacgaggcc cgctgaatgc cgtagctgcc
     3961 gcgcccgaac cagctggtgt tcagatagcg ctcgaggatg tcccgcttgc tcatccggtc
     4021 gtccagcttg atggccatga acatctcggt gagcttgcgg gagaaggtct gacgctggtt
     4081 cagataggcg ttcttcacat actgctgggt gatggtggag ccgccctggg tctcaccgcc
     4141 cgtcaccatc cgtgtcaccg cgcgcatcag tccgctcggg gacaccccgc ggtcggagta
     4201 gaagctggcg ttctctgcgg cgaggatcgc ccactggacc tgctccggca ccttcttcag
     4261 gggcacctcc tgccggttga ccggaccggt gcgggccatc tcggtgccgt ccgcccagta
     4321 gtagacgttg tcctgctggg tggcgaagtc gttgaggttc ttggggatgt cggtctggag
     4381 atacagaaag ctcagcagac cggtcagact gccgacgcag aacaggaaga acagggcgat
     4441 ctgacgccac gacggcagcc accgccccac cccacggcgc cccgtccggg ggtagtccag
     4501 ccggatccgc gccgcggccc agcggcgcag tcgcatcctc cggctcttcg aggacggctt
     4561 gcggcggtgc ttacggtgct tccccatggc gttgtcgctg cccggcacgt gtttccccca
     4621 cgctcgctcc gcgcgtgggc gcggcacaaa ggtgtgatct tccatccccc cagaccagac
     4681 cgatcctatt ttcccctctg aacatctgtc gccacagggc cccaaatttt tgtaacagtg
     4741 accatattca gcgacacgag ccacccggtg accattcgca ccgcctgtgc ggtggccgtg
     4801 gcatggccgc ctgtgcggtg agcgtgcgca cggccgtcgg cgaccgtccc gggggcgtac
     4861 accccctccg tgacaccccg aagctcaaat gaacctctga accgcgaggc ccggggattc
     4921 gtccttcctc tacggggtca tcaccccggc acgcgagaac acaccgcggg ggacgcggta
     4981 cggaacgagg agggggagcc atggtgcgcc tcggtaccgg aatcggctgg cgaccggaga
     5041 tcgccgatga catcgcgcga ctgcccggca tcgactgggt ggaggtggtg gcggagaaca
     5101 tctgccccga ccatgtgccg gacgcgctcc ggctgctgcg cgagcgcggg accacggtgg
     5161 tcccgcatgg ggtgtcgctc ggcctcgggg gagccgagcg gcccgaaccc ggccgtctgc
     5221 gcgcgctggc cgagcgggcc gaggcgctgg gctcgccgct ggtcaccgag cacatcgcgt
     5281 tcgtcagggc cgggggaccg ctgaccgcgt caccgtccat cgaggccggg catctgctgc
     5341 cggtgccgcg cacgcgtgac gcgctggacg tgctgtgcga gaacgtgcgc atcgcgcagg
     5401 acgcgctgcc ggtgccgctc gcggtggaga acatcgcggc gctgatctcc tggcccggcg
     5461 aggagatgac cgagggtcag ttcctggccg agctcgtgga gcgcacgggg gtgcggctgc
     5521 tcatcgacgt cgccaatctg cacaccaacc acgtcaaccg gggcgaggac ccggccaagg
     5581 cgctggacga gctgcccacg tcggcgatcg cctatgtcca tgtggcgggc ggggtggaga
     5641 aggacggcgt gtggcacgac agccacgccc atccggtgac ccagccggta ctcgacgtcc
     5701 tcgccgaact gtgcgcccgt acggcaccgc ccggcgtact gctggagcgc gacgaggact
     5761 tcccggcggc cggggagctg gcgggcgagc tggccgcgat ccgcgcggtg acggcggcgg
     5821 gggcggcggg ggccgcggag gcggggatgg cgacggcggg gacagcggcg gggagcgcga
     5881 agccggtcac ggcggcgggg gcagcggaag cggccacggc ggcgagggcc gcggaagcgg
     5941 ccacggcggc gcggaccggg gatgaggccc ccgccccggt gcgggcctcc gccgcggccg
     6001 acgaggccga caaggccgac gaggccgaca aggcccgcac cggggccgac gtccccgcgg
     6061 ccgacgcccc ggcggccgac gcccctgcgg ccgacgcccc cgcgcccgag acaccgctgc
     6121 ccgacgctcc gctgcccgag gcaccggtgc cggacgccgt ccgtgagcgg ctggcgctcg
     6181 cccagaccgc gctgctgtcc gcgctggtcg ggggcacccc gccgcccgag ggcttcgacc
     6241 gtcggcgact gcgggtgcag agcgcggcgc tgacggccaa gcgggcgtcc gtgatcgcga
     6301 aggtggcccc ggagctgccg gagatcctgg gcaccggcta ccgcccggcg ttcgcgcgat
     6361 acgccgaggg ccgcccgatg accggcggct accgccgcga cgcgctgtcg ttcgccgagc
     6421 atctgctggc ggacggccgg cccgacgact cggccgcccg gcgccggctg acgctgtggt
     6481 ggcaggagcg gtccgggccc gcgcccctgc cgtcccatcc ggcggcgcgg ttcgcccgcc
     6541 gggtccggct cgcgctgagc ggggggcggt gaccggcatg ggcacgatcg cgagcctggt
     6601 ctacgcggtg gtcaccgtgt cgtcggcgat actcatcacc ggtacggtcc gggcccggcg
     6661 caggagggtc tcggggccgg tacacgccca ggcgtacgac ctgctggagg ccgcgttcct
     6721 ggcgggcgga ccgggccggg tggcggacac cgtgatcagc acgatgtacg cggacggacg
     6781 gctggcgatc ggcggccccg gcgtggtctc ggtgcggcaa ccggtcgcgc gcggccccgt
     6841 cgaggaggag ttgctgcggc tgtccgccaa ctccccccac ggcgccctga cctggctgcg
     6901 ccgggaactg atgcgcagcg cggcggtgca ggagatcggc gaccggctgg cggggcgcgg
     6961 gctgatggta cggccggacg ccctgaggtt ctggcggcgc atggcgcgga cgcacatcac
     7021 gctctgcgcg gtgggagcct tcctggccat catgatcagc atcggacccg tcacctacga
     7081 cgggcctccg ctgatcttca tgatcgcccc cgcgctcttc atcggcttcc tcatcggcag
     7141 aatctgcgcc ggtgcgggca agcgcaggct gaccgccgcc gggaagcggg cgctggtggc
     7201 gtaccggagg gagtcggggg tcagcggacg ccatgcccgc gcccggtcca tcggccgtcc
     7261 ggcgacggtc tccgtggcgg tcgtcgtcgc gctgaccggg gcggccaccc tgacggacga
     7321 tccgcagctg cgggcccagc tgctggaggc gcagaaggta ccgagcggct cgggatcgcc
     7381 cggctactcg gggtcttcgg gcgattcggg gttctcggct tcggacggcg gcggatcgtg
     7441 gtgcggcggg gacggcggag gcgggggctc ctcgtgcggc ggctcgtcct gcggagggag
     7501 cagcggcgga ggctgcggcg gcggttcggg ctgcggaggg ggcggcggct gcggcggagg
     7561 tgggggcggc ggctgcggag gcggctgagc cgccacacaa gtggccgcac tgcggccagg
     7621 aggcatggcc gagcccgccg ggggcaggcg ggacgccggg ggcaagcgcg acgccggggg
     7681 caagcgcgac gccggggccg ggcgcgacgc cggaggcagg cgggacgccg gggggcgcgc
     7741 gacgccggac gcgggcgcga tgccgggggc aggcgcgacg ccggacgcgg gcgcgacgcc
     7801 ggacgcgggc gcgacgccgg acgcgggcgc gacgccgggg gcaggcgcga cgccggacgc
     7861 gggcgagggg ccggacgcag gcggccgacc gagcagcggc gggtcgagcc gcccagcccc
     7921 gggtgccgat ggcgaccggc aagaggtcct cgccgatccc agaccgcccg cccaccagca
     7981 tcccgcgccg cagctgtacg gatcgcccac gaccccacct cggtcccgag cagagttacg
     8041 gggtgtccgg cggaccatgc ggcggggccg catatcgatg ttttcccctc cccgcccctt
     8101 cccgataccg ggggctccgc ccccggaccc cggggtcaag gacggagacc cgccacgcgg
     8161 cggagccgca taccgatgcc gcgggcagga gcggaggccc tgccccgggg cccggggcag
     8221 ggccccgacc cggggcctgg ggcggagccc cggtttcggg ggctggggcg gagcccctat
     8281 acggggtctg gggcggagcc ccagttttgg gaaggggcgg ggaggggaac agtccgccgt
     8341 aggcggcaca atccgtcgcg ggtctgcgcg gtgcgcgcgc cgcattcgcg cgagtcgttc
     8401 atatcgtgaa aaccgtggcg cggtggcggc cgggtcgagt agaactcggc catgttgtgg
     8461 gtcctcttcc tgcttgtcgc gtggggcgcg gcgatcgtca gttgcacgcg tctgtgcctg
     8521 gcggcggtcg ccgccgctca gccgatggag cctgatccgg cggcggacgg gaagggcctg
     8581 agcctgtacg aggcggcgtt tttgtccggc ggcccgcggc gggtggggga gctggccctt
     8641 gtgacgatgt gccgcgagcg ccggctgctg ctcgcccaca ccggctgggt caccgtggtg
     8701 gacccggagg ggcgggacga gctggagcgg tccgtgatca ccgcgatagg gccggagggc
     8761 caggccctcg tgcggccggt gcgggccgcg ttcgccaccg cagccccggt gcgggcgctg
     8821 ggcgaccggc tggtggcggc agggctcgcg gtgcccgcct cggcccgtac gaatgtggcg
     8881 gtcgcggtgc ggcaggtacg ggcggcgtgc gcgctggtcc tggcgatggc ggtcgccgcg
     8941 ctgctgctgg tgccgcccgc ggcggggcgc ggtccggtgg cctcgtggtt catgctgccg
     9001 ctgctgctga cggtgagctg tctgctgatc gcccgggtcg aggcgcatcc gtactcacgg
     9061 tgggcctcgc ccgccggtca gcggctgctg ggccggatcg acgtgcctcc gcggcggacg
     9121 gtcgcccagc gttccggcgg cggcccggac ggcggtgccg acgacgccga tgtgctcacc
     9181 gcgctcgcgg tgcacggcac cgtcgcgctc tccgaccggg cgctgcgcgc ggcgctgaag
     9241 acgcattggg gtcattgacc ccgacaccgc ggcccggtgc tttacatggt catccgccgc
     9301 gccaaatatc cctttcatcg cgcgacgcac gaagggatgg ccagtgagag cagcagcgct
     9361 gtacggcatc ctcggaccgc tgtccctgtc gctgaccatg agcgctctgc tcgcgacgcc
     9421 cgccaccggc cgcccgctca ccgcgcccac cacccccgcc gccacggctg ccaccccgtc
     9481 caccagggcc gccacgcccc cggccggtgc ggcgggcgcg ccactcacgg cggcccccgc
     9541 ggcccgttcg gcggcccccg ccgaccccgt cgccgccacc gcgcccgtct ggaccacctg
     9601 gtccgggtcg tccgccgcgg ccgccgcccc gcccgctgac ccggccgcgg cccgcggggt
     9661 ggcgctcgcc ggccggcgcg cggcggagcg cggtgtcacc ttccgccgct gtccccgcgc
     9721 cgagtcgctg gccgcgccca tcacctgcgg aaaggtctcc gtaccgctcg actacgcccg
     9781 tccgcgcggc aagcgcgtct ccctcaccgt cagccgggtc cgggcgagcg gccccaaggc
     9841 gcggcgtcag ggcgccctga tctacaaccc gggcggaccg ggcgcgtccg gtatggcgtt
     9901 ccccgagtac gcggccgaga agtccttccg ccgcttggcc gccgtctacg acttcgtcgg
     9961 ctacgcccca cggggcgtgg gccgctccgc cccgctctcc tgccagcggc cctcggccta
    10021 cgccaaggcg ccgaccagtt ccccgccgca ccccaccgcc gccttcaaac ggcggctggt
    10081 ggcgcgggcg cgcgcctatg tgcggggctg tgtgcgccgc tcgggccgcg cgctcgcgca
    10141 ctacacgacg ctgaacaacg cccgcgatct ggaggtgctg cgcgcggcgc tgggcgagcg
    10201 gcggctgacc ttcgtgggcg cctcgtacgg cacgtactac ggggccgtgt acgccacgct
    10261 cttcccgggg cgggtgcgcc ggatggtctt cgacagcgtg gtcaacccgg ccccctggca
    10321 gatctggtac cgcgacaacc tcgaccagtc gctggccttc gagcggcgct ggcgggactg
    10381 gcgccgctgg gtcgcccggc accaccgcgt ctaccacctg ggcgccaccg cggaccgggt
    10441 gctcgcctcg tacgagaagg cgcggcggcg gctggcgcgc aagccggcgg gcggggtcgt
    10501 cggcaccgcc cagctccaga gcgcgttcct caggaccggc tacaacgacg cctactggga
    10561 tccggccgcc cgggcgctgg ccgcctatct gctcggtgac tccaagccgc tgatcgcgct
    10621 cgccgcgccc gatcgggcgc aggcggccga cgacgagaac ggcaacgccg tctacaccgc
    10681 cgtggagtgc aacgacgcgc cctggccgcg cgagtgggcc acctgggacc gcgacaacac
    10741 cgccctcgcc cgccgcgcgc ccttcgagac ctgggacaac gtggcgatga acctgccgtg
    10801 cgccttctgg ccggtgaagc cgggccgccc cctcgacgtg gggaacctgg aggccgtggg
    10861 cccccggacg gggcgggtcg cgctgccgcc ggtgctgctg ctgtcagcgg agcgggacgc
    10921 ggccaccccg tacgcggggg cgaaggagct gtggcaccgg ctgcccggct cctcgctggt
    10981 gaccgagcgg aaggcgggga cccacggcct gtggggcggt ccgaacggct gcgtcaaccg
    11041 ccatgtggac acctatctgc tgaccggtag gacccccggc cggtccgcct actgcgcccc
    11101 gcgcccggcg cccgaaccac agccggaacc ggccccgctg cccgattcgg gcaagcagcc
    11161 ggccgcgctc ccgggcacgc cgtaagaggg cgccgaagcc cgtgcgggac ggccgccgga
    11221 ccgtcccgcg gcggccttac ggccacaccg gcggccgtaa ggtcacccgg cggctggcaa
    11281 ggatcccggc gcccgccgta agccgtcccg gcccgccgta aaggcgtccc cggctgccgt
    11341 aagggcgtca ggctcaggcc agaccggcga ccagctcgga gaccgccttg cgccgcccgg
    11401 tgaagaacgg cacctcctcg cgcacatggc ggcgcgcctc ggagccgcgc agatggcgca
    11461 tcaggtcgac gatccggtgc agttcgtccg cctcgaaggc caggatccac tcgtagtcgc
    11521 cgagcgagaa ggacgcgacc gtgttggcgc gcacatcggg gtagccgcgc gc
//
