LOCUS       NZ_CP025018            10936 bp    DNA     linear   CON 12-FEB-2024
DEFINITION  Streptomyces sp. M56 chromosome.
ACCESSION   NZ_CP025018
VERSION     NZ_CP025018.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. M56
  ORGANISM  Streptomyces sp. M56
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 11742376)
  AUTHORS   Kim,K.K.Hyun., Klassen,J.L., Ruzzini,A.A.C., Beemelmanns,C.C. and
            Clardy,J.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-NOV-2017) Leibniz Institute for Natural Product
            Research and Infection Biology - Hans Knoll Institute,
            Adolf-Reichwein-Strasse 23, Jena 07745, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-AUG-2014
            Assembly Method                   :: HGAP v. AUG-2014
            Assembly Name                     :: M56v2
            Expected Final Version            :: yes
            Genome Coverage                   :: 94.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/12/2024 06:37:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,558
            CDSs (total)                      :: 9,473
            Genes (coding)                    :: 9,167
            CDSs (with protein)               :: 9,167
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 86 of 306
            Pseudo Genes (incomplete)         :: 261 of 306
            Pseudo Genes (internal stop)      :: 28 of 306
            Pseudo Genes (multiple problems)  :: 61 of 306
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:58:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2319041
            Orig. end                         :: 2329977
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP025018.1.
            Annotated using prokka 1.11 from http://www.vicbioinformatics.com.
            Bacteria and source DNA available from Christine Beemelmanns.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10936
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="15"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10936
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10936
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5936](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5936)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            685..2199
                     /locus_tag="CFP59_RS08795"
                     /old_locus_tag="CFP59_01735"
     CDS             685..2199
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056770.1"
                     /locus_tag="CFP59_RS08795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_01735"
                     /product="NADH-quinone oxidoreductase subunit NuoF family
                     protein"
                     /protein_id="WP_069867543.1"
                     /transl_table=11
                     /translation="MITTKPRLACVDPPRLLAGLDEVYRLDRVGHLTVHGTMPALRADE
                     LVALAENIDLRGRGGAGFPFAKKVQAVVESAGRREGRCAVVVNGSEGEPSCLKDTALLL
                     HTPHLVIDGAVLAAEAIGAEEVAIAVHRPDVEESLAAAIAERGPSGVPVRVSRTPDRFV
                     AGEGGAVINGISGAPAIPNGRKIRTSDSGLSGLPTLLSNTETFAQLAVAARMGALPYRS
                     VGLPTEPGTTLLTVGGKYVMETPTGVPLTYVLELCGLTAGQAVLVGGYHGKWLDPRSIN
                     AATISRESMKKYGARLGAGAVLPIPENTCPLGETARVARWLAAETAGQCGPCFIGLPAI
                     ADAIGQVERGGGQAAIDNVRAYVNSVKKRGACSHPDGTAGFVTTALEAFPEEFEAHALG
                     AGCGRPTMGVLPLPEDALPLALPPAPLNPSARDDRSQRGPMEKLLVDWSLCQGHGLCAD
                     ILPPEVLTLGIDGYPASASMEVPRQLRAQAVRAVRRCPALALRIEE"
     gene            2410..3384
                     /locus_tag="CFP59_RS08800"
                     /old_locus_tag="CFP59_01736"
     CDS             2410..3384
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056769.1"
                     /locus_tag="CFP59_RS08800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_01736"
                     /product="SCO0930 family lipoprotein"
                     /protein_id="WP_100805457.1"
                     /transl_table=11
                     /translation="MEKRRHIAFAGVSVAMVLLTAACGSSKDNTAGSANVQPAGGSQTV
                     GAGASASAGAVAGAGGGYEAGGDTGAAAGGAAERTGPAKQLAVKEDAKLGKFITDGKGW
                     TLYRFDEDTPKPAKSNCNDDCATKWPAVPADDATATTGIDSAKLGSVTRSDGTKQLTLA
                     GWPVYRFAGDDKPGDTKGQGVGGTWNALAPDGKPAGKTAATDDSLQLMVNNNAELGKII
                     VDGDGMTVYRFNKDSAWPMKTGCLGACLDTWKPVKAVDTTKVAGVDAAKVTSFKRPDGT
                     KQAAFDCWLLYTFTGDKKPGDINGQGVKGTWFAVTDKGKKAGQ"
     misc_feature    complement(2423..2433)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 17.67"
                     /tool="antismash"
     gene            3476..3991
                     /locus_tag="CFP59_RS08805"
                     /old_locus_tag="CFP59_01737"
     CDS             3476..3991
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056768.1"
                     /locus_tag="CFP59_RS08805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_01737"
                     /product="hypothetical protein"
                     /protein_id="WP_100805458.1"
                     /transl_table=11
                     /translation="MSLALRATAVCAGLLFLAGCGGNDQAHGARHEQKKISPATKEFAE
                     PGTPAPLTKLADAIGCKATVITEADELRQGACGSGAKRFTMVTFATDKGQHDWLTTSKD
                     YGGMYLVGKRWSVTGLSMNSLEPLREKIGGSLEKGMSHGASHGGKKQEDGMEGMDGSAS
                     HGGHDGKK"
     gene            complement(4035..4712)
                     /locus_tag="CFP59_RS08810"
                     /old_locus_tag="CFP59_01738"
     CDS             complement(4035..4712)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056767.1"
                     /locus_tag="CFP59_RS08810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_01738"
                     /product="HAD family phosphatase"
                     /protein_id="WP_069867278.1"
                     /sec_met_domain="HAD_2 (E-value: 8.9e-22, bitscore: 77.1,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAEKFLGWTPAAIVFDCDGTLMDSERHWEEAREVVLRSHGTAPD
                     EEFARRTKGLHYTECGRMLAEFAGRPELAEEMTEQLLDAFRRLVADDPVTMPGAPQLVA
                     KAATFAPLAVASNCPRDVVEDGLAKAGLLRYFGHVLVPEGEVRPKPYPDVYLEAARLCG
                     AAPADALAVEDSHCGFLSASRAGLRVLGVGPRKPVDESPPVDVWVSTLADPDLVKWADS
                     RAA"
     misc_feature    4860..4880
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.82"
                     /tool="antismash"
     gene            complement(5001..5936)
                     /locus_tag="CFP59_RS08815"
                     /old_locus_tag="CFP59_01739"
     CDS             complement(5001..5936)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056766.1"
                     /locus_tag="CFP59_RS08815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_01739"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_069867279.1"
                     /sec_met_domain="AfsA (E-value: 8.3e-19, bitscore: 66.5,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVIVREAHGLSEPSGPAGRPLSWSRTVAREAVHRVSVAEVLLTDV
                     RTHGDNRFEAAAQWPRSHPTFPHGGDGRHHPLMIAETLRELGIYIPTRYYAVPAGAHFL
                     IRDISYRLDPETEPRAPYGASDITCRAAVTDIRTSPCGRFVTGMRLDVVLSAGGKLFAW
                     AGGTARFLDGTTYAETRAAAHGHTARRRLRAAVRPSPAQLAVPAARDVLVVRDGGVVYL
                     DPADPRHPFFFDHWSDHMPGLVLLEGARQAAALATGGTLIRPVVCRMKAIRFTESYPPA
                     VVECTSHGRTCVFRLRQAGICTAVGVLQYL"
     misc_feature    complement(5935..5948)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 15.6"
                     /tool="antismash"
     misc_feature    5936..5949
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 15.9"
                     /tool="antismash"
     misc_feature    complement(5938..5951)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.73"
                     /tool="antismash"
     misc_feature    complement(5941..5954)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 14.63"
                     /tool="antismash"
     misc_feature    5947..5956
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.18"
                     /tool="antismash"
     misc_feature    complement(6018..6034)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 17.49"
                     /tool="antismash"
     gene            6045..6686
                     /locus_tag="CFP59_RS08820"
                     /old_locus_tag="CFP59_01740"
     CDS             6045..6686
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056765.1"
                     /locus_tag="CFP59_RS08820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_01740"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_069867280.1"
                     /transl_table=11
                     /translation="MQERAEQTRRSLLEAAAFLFDERGYAGTSISDITSRSGHTSGAIY
                     FHYTSKERLALAVVEEHFASWPPLIERYTALDSPPLEQLVRLSFAVARAFRDDIVVRAG
                     ARLWAERTLIEAPMPPPFVGWIDAVGQMMEKAGAEGDLAPHVDPLPAARTVVCAFFGLH
                     TVSEALDGRRLVEDRLADLWTLLLPSLQARPGDPAGLLALARADSPPAPE"
     gene            complement(6846..7529)
                     /locus_tag="CFP59_RS08825"
                     /old_locus_tag="CFP59_01741"
     CDS             complement(6846..7529)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (7-163). E-value: 1.3e-10. Score:
                     33.4. Matches aSDomain:
                     nrpspksdomains_CFP59_RS08825_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056764.1"
                     /locus_tag="CFP59_RS08825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_01741"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_093700783.1"
                     /transl_table=11
                     /translation="MAEPRHAVVTGVSSGIGAAIATRLLADGWRITGLSRTAPAHTVAG
                     SHWIPADLSRPEILPELLAPVSGVDAIVHAAGVQRSARLGELEPEDGALMWRIHVQAAG
                     VLVDTLVDRIADGGRVVLVGSRTMTGVPGKSQYAATKAALPALARSWAAELAPRAVTVN
                     VVAPGPTDTPMLRDPGRSRTPPVLPPLGRLVRPEEVAGLTSFLLGPEGGAVTGQTLVMC
                     AGASL"
     aSDomain        complement(7041..7508)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CFP59_RS08825_PKS_KR.1"
                     /evalue="1.30E-10"
                     /label="CFP59_RS08825_PKS_KR.1"
                     /locus_tag="CFP59_RS08825"
                     /protein_end="163"
                     /protein_start="7"
                     /score="33.4"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VVTGVSSGIGAAIATRLLADGWRITGLSRTAPAHTVAGSHWIPAD
                     LSRPEILPELLAPVSGVDAIVHAAGVQRSARLGELEPEDGALMWRIHVQAAGVLVDTLV
                     DRIADGGRVVLVGSRTMTGVPGKSQYAATKAALPALARSWAAELAPRAVTVN"
     gene            complement(7522..8727)
                     /locus_tag="CFP59_RS08830"
                     /old_locus_tag="CFP59_01742"
     CDS             complement(7522..8727)
                     /EC_number="3.2.1.18"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009712848.1"
                     /locus_tag="CFP59_RS08830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_01742"
                     /product="exo-alpha-sialidase"
                     /protein_id="WP_100805459.1"
                     /transl_table=11
                     /translation="MTADHDTALPPTDGRLRVRPGDPACQEAFLPAPAVQNHAANLTVL
                     PGGDLGCVWFGGTQEGVPDISVWFSRLAPGSDTWTEPVRLSGDATRSEQNPLLFPTPSG
                     ELWLLYTAQRAGDQDTAEVRLRVSADAGATWDAPRTLFPATEAGGVFIRQPVAVLDSGR
                     WLLPVFHCVATPGAKWAGDHDTSAVMISDDQGRTWRERSVPGSTGCVHMNVHQLPDTTL
                     LALFRSRWADAVYRSHSTDGGETWSEPVRTELPNNNSSVQYVPLADGRLALVYNHSSRA
                     DATARRVSLYDEIDDAGHTARAPAPATARDASAPGAFWGAPRAPMTLALSSDNGLTWPV
                     RRDLDTGDGHCLTNNSRDRLNRELSYPTIRQGPDGTLHIAYTYHRQAIKYVRVAPDWAA
                     DG"
     gene            complement(8977..9996)
                     /locus_tag="CFP59_RS08835"
                     /old_locus_tag="CFP59_01743"
     CDS             complement(8977..9996)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1039: aldo/keto reductase family oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056762.1"
                     /locus_tag="CFP59_RS08835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_01743"
                     /product="aldo/keto reductase"
                     /protein_id="WP_079259853.1"
                     /transl_table=11
                     /translation="MNAHAQTHTTAHASPGGVALPGTPVPLSRLVLGTMTFGDTVDRAG
                     AAAMLDTALDAGVTGVDTANAYAGGTTETILAELLPGRRDRIVLATKAGMPHPDAGDHS
                     PLSARGMRAALEGSLRRLGTDHVDLFYLHQPDRSTPLEETLGTVAEFLAEGKIRALGVS
                     NYAAWQIAEITHTAERVGAPRPVVAQQLYNLLARRIEEEYREYAATSGLRTMVYNPLGG
                     GLLTGRHTFQERPESGRFGDSRVAAMYRERYWDAGLFEAVRDLGRIADEASIPLTDLSL
                     RWLLFRDGVDALLLGGSRIEHLRSNLDAATAGPLPDDVVAACDEVGARLRGPMPAYNR"
     misc_feature    10468..10484
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 19.95"
                     /tool="antismash"
ORIGIN
        1 gttcctcgac ttcggcgcgg gtgtgctcgc actggtctgt ctcacctcgg cggtgctgtg
       61 ggggctcgcc gcgaccgacc agatgatcct gcacaccagg cagcggctga tggcccaggc
      121 gatccaccgg gcggccgcgg tcgccgggtt gctgttcctg gccgttcaca tctgggtcaa
      181 ggtcgcggag agccacacca acgccgcctc cgccgtacta ccgttcgcgg acgcaaatca
      241 gccggtactc atcgggctcg gcaccatcgc cggctatctc ttcgtgatcg ccgcggtgac
      301 cggcgccgtg cgcagctcgt tcgcctcggc gggcaactcc cgatggcggt accgctggtg
      361 gcgggcgctg catgtgggcg cctaccccgc gtggtgcgcc gccctgatcc acggtctgaa
      421 agcgggccgc gccgccaaga tctgggccac ctacggctac atcgcggcgc tcgccggtgt
      481 ggccatcgcg ctgtggctgc gtctggtcca gcggcggcgc caggacatcg accgggcgga
      541 cggcatcgag gtcaggaacc cgcccgcccg cagcatgcag gagggccgga cccggcgggc
      601 cgatcccacc gtgccgcgcc agcgtgcggc cgagcccggc ccgcgtgaca gtcagccgct
      661 cggtgcctct cgcgggtggg accggtgatc acgaccaaac ccagactcgc ctgtgtcgat
      721 ccgccccggc tgctggccgg gctcgacgag gtgtaccggc tcgaccgcgt cggccatctg
      781 accgtccatg gcaccatgcc ggccctccgg gcggacgagc tcgtggcgct cgcggagaac
      841 atcgatctgc gcgggcgcgg tggcgccggt ttccccttcg ccaagaaggt ccaggccgtg
      901 gtggagtcgg ccggccggcg cgagggccgc tgtgccgtgg tggtcaacgg cagcgagggc
      961 gagcccagtt gtctcaagga caccgcgctg ctgctgcaca ccccgcatct ggtgatcgac
     1021 ggcgccgttc tggccgccga ggcgatcggc gccgaggagg tcgccatcgc ggtgcaccgc
     1081 ccggatgtcg aggagtccct ggccgccgcg atcgccgagc gcggccccag cggggtcccg
     1141 gtgcgggtga gccgcacccc ggaccggttc gtcgccggtg agggcggcgc ggtcatcaac
     1201 ggcatcagcg gcgccccggc catccccaac ggccggaaga tccggaccag tgacagcggg
     1261 ctcagcgggc tgcccacgct gctgtccaac accgagacct tcgcccagct cgcggtggcc
     1321 gcgcggatgg gcgccctgcc gtaccgctcg gtggggctgc ccaccgaacc gggcaccacg
     1381 ctgctgacgg tcggtggaaa gtacgtgatg gagacgccca ccggggtgcc gctgacctac
     1441 gtccttgagc tgtgcggcct caccgccggc caggcggtgc tggtcggcgg ctaccacggc
     1501 aagtggctgg accccaggag catcaacgcg gccacgatct cccgcgagtc gatgaagaag
     1561 tacggcgccc ggctgggcgc cggggcggtg ctgcccatcc ccgagaacac ctgcccgctc
     1621 ggcgagaccg cgcgggtggc gcgctggctg gccgccgaga cggccggtca gtgcgggccg
     1681 tgcttcatcg ggctgccggc gatcgcggac gccatcggcc aggtggagcg cggcggcggc
     1741 caggccgcga tcgacaatgt gcgcgcctac gtcaactcgg tgaagaagcg cggggcgtgc
     1801 agccatcccg acggcaccgc cggtttcgtg accaccgcgc tggaggcgtt ccccgaggag
     1861 ttcgaggcac atgcgctcgg cgccgggtgc ggacggccca cgatgggtgt gctgccgctg
     1921 ccggaggacg cgctgccact ggccctgccg cccgccccgc tgaacccctc ggcgcgcgac
     1981 gaccgctccc agcgcgggcc gatggagaag ctcctggtgg actggtcgct gtgccagggc
     2041 catgggctgt gcgcggacat cctgccgccc gaggtgctga cgctcgggat cgacggctat
     2101 ccggcctcgg cctcgatgga agtgccccgg cagctgcggg cgcaggcggt ccgcgcggtc
     2161 cgccgctgcc cggccctggc gctgcgcatc gaggagtagc cggaggcgga tccatctggc
     2221 accaccgcac caccgacacc gcggcggccg tgagaaacgg ccgccgactg ctgtgcgctc
     2281 agcgatccgg agttttttgc ctccggtgac aaattcgcag gccgctgaac gccggtccgc
     2341 atgggctccg tatgcacatg acgtcggcgc aatcaccgcc ggaccgatcg agattgcaga
     2401 ggaatggtca tggagaagcg gcgtcacatc gcattcgccg gggtgtcggt agcgatggtt
     2461 ctgctgacgg cggcgtgcgg cagcagcaag gacaacaccg cgggcagcgc gaatgtgcag
     2521 cccgcgggcg gcagccagac cgtgggtgcg ggcgcctccg cctcggcggg cgcggtggcc
     2581 ggcgccggcg gcgggtacga ggcgggcggc gacacgggcg cggccgcggg cggcgcggcc
     2641 gagcggaccg gtccggcgaa gcagctggcg gtcaaggagg acgccaagct gggcaagttc
     2701 atcaccgacg gcaagggctg gacgctctac cggttcgacg aggacacccc caagccggcc
     2761 aagtccaact gcaacgacga ctgcgccacc aagtggcccg cggtccccgc ggacgacgcc
     2821 acggccacca ccggtatcga ctccgccaag ctgggctcgg tcacccgctc cgacggcacc
     2881 aagcagctga cgcttgcggg ctggccggtc taccgcttcg cgggcgacga caagccgggt
     2941 gacaccaagg gccagggcgt gggcggcacc tggaacgcgc tggcgccgga cggaaagccg
     3001 gccggaaaga ccgcggccac ggacgacagc ctccagctga tggtcaacaa caacgccgag
     3061 ctcggcaaga tcatcgtgga tggcgacggt atgaccgtct accggttcaa caaggacagc
     3121 gcctggccga tgaagaccgg ctgcctcggt gcctgcctgg acacctggaa gcccgtcaag
     3181 gccgtggaca ccaccaaggt cgccggggtc gacgcggcga aggtcacctc cttcaagcgc
     3241 cccgacggca ccaagcaggc cgcgttcgac tgctggctgc tgtacacctt caccggggac
     3301 aagaagcccg gcgacatcaa cggccagggc gtgaagggca cctggttcgc ggtcaccgac
     3361 aagggcaaga aggcgggcca gtagggcgga caccatactg acggtccacc gcggcgggtg
     3421 gtccgaggcc atccgggccc ggaccgcccc gcctgacgtt ctacgggagg ttgatgtgtc
     3481 actcgcactc cgtgccaccg cggtctgcgc cggtttgctg ttcctggccg gctgcggtgg
     3541 aaacgaccag gcacacggcg ccagacatga gcagaagaag atatcccccg ccaccaagga
     3601 gttcgccgag cccggcacgc ccgcgccact gacgaagctc gccgacgcca tcggctgcaa
     3661 ggccacggtc atcaccgagg cggacgagct gcgccagggc gcctgcgggt ccggtgcgaa
     3721 gcggttcacg atggtcacct tcgccaccga caagggccag cacgactggc tgacgacgtc
     3781 gaaggactac ggggggatgt acctggtcgg caagcgctgg tcggtcaccg gcctgtccat
     3841 gaactcgctg gagcccctgc gcgagaagat cggcggatcc ctcgagaagg ggatgtccca
     3901 cggcgcctcc cacggcggga agaagcagga ggacggaatg gagggcatgg acggctcggc
     3961 gagccatggc ggccacgacg ggaagaagtg acgcgcggcg cccgccgacc aggcggtgtg
     4021 cgcgggtcag cccctcaggc cgcccgggag tccgcccact tgaccaggtc cgggtcggcg
     4081 agggtggaga cccacacatc gaccggcggg gactcgtcca ccggcttgcg cggcccgacg
     4141 ccgagcaccc tcagcccggc gcgcgaggcg gacagaaacc cacagtggga gtcctccacg
     4201 gcgagggcgt ccgcgggggc cgcaccgcac agccgggcgg cctccagata gacatcggga
     4261 tacggtttgg gccgcacctc cccctcgggg accagcacat ggccgaagta gcgcagcagc
     4321 ccggccttgg cgagcccgtc ctcgacgacg tcccgcgggc agttgctggc cacggccagc
     4381 ggcgcgaagg tcgcggcctt cgcgaccagt tggggcgccc ccggcatggt gaccggatca
     4441 tcggccacca gccggcggaa cgcgtccagg agttgttcgg tcatttcttc ggcgagttcg
     4501 gggcgcccgg cgaattcggc cagcatgcgg ccgcattcgg tgtagtggag ccccttcgtg
     4561 cgccgggcga actcctcgtc gggggcggtt ccatggcttc tgagcaccac ttcacgcgcc
     4621 tcctcccagt gtcgttcgga atccatcagg gtgccgtcgc agtcgaagac gattgcggct
     4681 ggggtccatc cgagaaactt ttcggctgtc atctgtctgc cgctccatgg gaggtggagg
     4741 aatcggcgca gggtgtgcct gtacctgcgg gggccgcgca gtggaaatgt cctggcggct
     4801 cagaagggcg ctgggcgccg accgctgttg tcgtacaggc cgcgagaatc gcctgcccga
     4861 aacataacga tcactacgtt agtttgatga cccgcagtaa gcaaggggtc accgagtgtc
     4921 ggcgttttgt tcgtcgttca gtccgttcgt cgccaaagag ttcgtcgcac agtctgttcg
     4981 tcgcacggcg cgttcgtcgc tcagaggtac tggagcacgc cgaccgccgt acagataccg
     5041 gcctggcgca gccggaagac gcacgttctg ccgtgggagg tgcactcgac cacggccggc
     5101 ggatacgact cggtgaagcg gatcgccttc atccggcaga cgacgggccg gatcagcgtc
     5161 ccgccggtcg ccagcgccgc cgcctggcgg gcgccctcca gcagcaccag ccccggcata
     5221 tggtcgctcc agtggtcgaa gaagaacgga tggcgggggt cggcggggtc gagatagacc
     5281 actcccccgt cccgcaccac caggacgtcc cgggccgcgg gaacggccag ttgcgcaggt
     5341 gagggccgta ccgcggcgcg cagccgacgg cgcgcggtgt gtccgtgggc ggcggcccgg
     5401 gtctccgcgt aggtcgtgcc gtcgaggaag cgcgcggtgc ccccggccca ggcgaagagc
     5461 ttgccgcccg ccgacagcac gacatccagc cgcatcccgg tgacgaaacg cccgcagggg
     5521 ctggtgcgga tgtcggtgac ggcggcccgg caggtgatgt cggaggcgcc gtacggcgcc
     5581 cggggctcgg tctcggggtc cagccggtag gagatgtccc ggatcaggaa atgcgccccc
     5641 gcgggcaccg cgtagtaccg cgtggggatg tagatcccca gttcgcgcag ggtttccgcg
     5701 atcatcagcg gatggtgacg gccgtccccg ccgtgcggga aggtgggatg ggaccgcggc
     5761 cactgggccg cggcctcgaa tcgattgtca ccgtgcgtac gcacatcggt gagcagcacc
     5821 tcggccaccg agacccggtg caccgcctcc cgcgccaccg tgcgggacca gctgagcggc
     5881 cgcccggcgg gaccggacgg ctccgaaagg ccatgagcct cacgaacgat caccataaaa
     5941 tactaataat gtttgacctg tctcgcctca tcaccatcac tggccgaaag tccgctaagt
     6001 tctccccgtc acatccgatg tgctggtgta aggacggcga acacgtgcaa gaacgggccg
     6061 aacaaacacg ccgatccctt ctggaggccg ccgcattcct tttcgacgaa cggggatacg
     6121 ccggaacgag catcagcgac atcacgtcca ggtccggcca caccagcggc gcgatctatt
     6181 tccattacac gagcaaggaa aggctcgcgc tggccgtggt ggaggagcac ttcgcctcct
     6241 ggcccccgct gatcgagcgc tacaccgccc tcgactcgcc tccgctggag caactggtgc
     6301 ggctcagctt cgcggtggcc cgcgccttcc gcgatgacat cgtggtccgc gccggagccc
     6361 ggctgtgggc cgagcgcacc ctgatcgagg cgcccatgcc accgccgttc gtggggtgga
     6421 tcgacgccgt gggccagatg atggagaagg cgggggccga gggcgatctg gccccgcatg
     6481 tcgatccgct gcccgccgcc cggaccgtgg tctgcgcctt cttcgggctg cacaccgtct
     6541 ccgaggccct ggacgggcgg cggctggtcg aggaccgcct ggccgatctg tggaccctgc
     6601 tgctgccctc gctccaggcc cgacccgggg atccggcggg gctgctggcg ctggcccgcg
     6661 ccgactcgcc gcccgcgccg gaataaggcg cccgcgccga cccccgccca cgtcagagtg
     6721 aagcaccccc ggcggctccc cggcccggtc agagcgaggc gcccgcggcc gcgtccacgc
     6781 ccggccccct gcggtcactc ccccgtcccg tcagagcgag gctcctgcgg ccacccccgg
     6841 tcccgtcaga gcgaggcacc cgcgcacatc accagcgtct ggccggtgac cgcgccgccc
     6901 tccgggccca gcaggaacga cgtcagtccc gccacctcct ccgggcggac cagccggccc
     6961 agcggtggca gcaccggcgg ggtgcggcta cggccggggt cgcgcagcat gggggtgtcc
     7021 gtcggccccg gcgccaccac gttcaccgtg accgcgcggg gcgcgagctc ggccgcccag
     7081 gaccgggcca gggcgggcag cgccgccttg gtggcggcgt actggctctt gcccgggacg
     7141 ccggtcatcg tccggctgcc caccagcacc acccggccgc cgtccgcgat ccggtccacc
     7201 agcgtgtcca ccaggacgcc cgccgcctgc acatggatcc gccacatcag cgccccgtcc
     7261 tcgggctcga gctcccccag ccgcgccgag cgctgaacgc ccgccgcgtg cacgatcgcg
     7321 tccaccccgg acaccggggc gagcagctcg gggaggatct ccggccggga cagatcggcc
     7381 gggatccagt gcgaccccgc gaccgtgtgc gcgggggccg tacggctcag cccggtgatc
     7441 cgccacccat cggccagcag ccgggtggcg atggcggcgc cgatacccga gctgacaccg
     7501 gtgaccacgg cgtggcgggg ctcagccatc ggccgcccaa tccggtgcga cgcggacgta
     7561 tttgatggcc tggcggtggt aggtgtacgc gatgtgcagg gtgccgtcgg ggccctgccg
     7621 gatggtgggg taggacagct ctcggttgag ccggtcgcgt gagttgttgg tcaggcagtg
     7681 gccgtcgccg gtgtccagat cgcgccgtac gggccaggtc aggccgttgt cggaggacag
     7741 cgcgagggtc atcggggcgc gcggtgcgcc ccagaaggcg ccgggtgccg aggcgtcccg
     7801 cgcggtggcc ggggcggggg cccgagcggt gtgccccgca tcgtctatct cgtcgtagag
     7861 ggagacgcgg cgggccgtgg cgtccgcccg gctgctgtgg ttgtagacca gtgcgagccg
     7921 gccgtcggcc agcgggacgt actgcacgga ggagttgttg ttgggcagtt cggtgcggac
     7981 cggctcgctc caggtctccc cgccgtcggt gctgtgcgag cggtacaccg cgtccgccca
     8041 gcggctgcgg aagagggcca gcagggtggt gtccggcagt tggtggacgt tcatgtgcac
     8101 acagccggtc gagcccggca cggaccgctc acgccaggtc cgcccctggt cgtcgctgat
     8161 catcaccgcg ctggtgtcat ggtctccggc ccatttggcc ccgggggtgg ccacacagtg
     8221 gaacaccggc agcagccacc gcccggagtc cagaacggcc accggctgac ggatgaacac
     8281 cccacccgcc tcggtggccg ggaagagcgt acgcggcgcg tcccaggtgg cgcccgcgtc
     8341 ggcggacacc cgcagccgca cctccgccgt gtcctggtcg cccgcccgct gcgcggtgta
     8401 gagcaaccac agctcaccgg acggggtggg gaacagcaac gggttctgct ccgagcgggt
     8461 ggcgtcgccg gagagccgga ccggctccgt ccaggtatcg gagcccgggg cgagccggga
     8521 gaaccacacg gagatgtccg gtacgccctc ctgggtgccg ccgaaccaca cacagccgag
     8581 atcccctccg ggcagcacgg tgagattggc ggcatggttc tgcacggcgg gcgcgggcag
     8641 aaacgcctcc tggcacgccg ggtcaccggg gcgcacccgg agccgtccgt cggtcggcgg
     8701 cagagcggtg tcatggtcgg cggtcatggt cgccgggctc ctcaagattg cggtgatacg
     8761 ggaatgtgta cggccgggcg agggccctcc cacggcaaca ccctgcggcc acggcgcacg
     8821 attcggaggc gaagcgaggc gccgcggccg caccgcgcgg ggcggcacga cgcggggcgg
     8881 ggcgctgccg tccgggagcc ggggcggagg ggcgttcggg gtccaggggc ggagcccctt
     8941 ggttcgggga ggggcggggt ggggaaaacc acgggcctac cggttgtacg cgggcatcgg
     9001 cccacgcaac cgcgccccca cctcgtcaca cgccgccacc acatcatccg gaagcggccc
     9061 cgccgtggcc gcgtccaggt tggaccggag gtgctcgatc cgggagccgc ccagcaggag
     9121 ggcgtccact ccgtcgcgga acagcagcca gcgcagcgag aggtccgtca gcgggatgga
     9181 cgcctcgtcc gcgatccggc ccaggtcgcg tacggcctcg aagagccccg cgtcccagta
     9241 gcgctcccgg tacatggcgg ccacccggga gtcgccgaag cggcccgact ccggccgctc
     9301 ctgaaacgta tgacgccccg tcagcagccc gccgccgagc gggttgtaga ccatggtgcg
     9361 cagtccactg gtggccgcgt actcccggta ttcctcctcg atccgccgcg ccagcaggtt
     9421 gtagagctgc tgggccacca ccgggcgcgg ggcgccgacc cgctcggcgg tgtgggtgat
     9481 ctcggcgatc tgccaggcgg cgtagttgga gacgccgagc gcgcggatct tgccctcggc
     9541 gaggaactcg gcgaccgtgc ccagcgtctc ctccagcggt gtggagcggt cgggctggtg
     9601 gagatagaac aggtcgacat ggtcggtgcc cagacggcgc aggctgcctt cgagggccgc
     9661 gcgcattccg cgcgcggaca gcggtgagtg gtcaccggcg tccgggtgcg gcataccggc
     9721 cttggtggcg agcacgatcc ggtcgcggcg gccgggcagc agctcggcca ggatcgtctc
     9781 ggtggtgccg cccgcgtagg cgttggcggt gtccacgccc gtcaccccgg cgtccagcgc
     9841 ggtgtccagc atcgcggcgg cgcccgcgcg gtccaccgtg tcgccgaagg tcatggtgcc
     9901 aagcaccagc cgcgagagcg gcaccggggt tccggggagg gcgaccccgc cgggcgacgc
     9961 gtgggcggtg gtgtgggtct gcgcatgcgc gttcactgcg ttgctcctgg ttcctgtggt
    10021 tcccgtggtt cccgtggttc ccgtgggtct tgtggttccc atagttcccg ttcccgtggt
    10081 tcctggtcgg tcgcgatcgt ggacacggga gggccggaac gtagggggca cgggtcagcg
    10141 ttgacctctc ggcagttccg aggcgagcga ccggttcgct cgactcgctc cactcgctcg
    10201 acccgtgcgg cgcccggatg acggccgtac gggtcaaagt gtcgcgacgg tcaccgtgac
    10261 atgactgcgc gctttacgca atagctctcg cgcacttcgc gcaaagtatg tcatcatcgg
    10321 gaccacggag acgatgtcgc cagcagcgcg tcgctccgtc ggtcatggtc attcgaggag
    10381 gtaccggtgg acatggcggc cagccggagg ctgttggcca tcgcggatga cctgtcaggc
    10441 gccgcggaag tggcggcggc gcttcattcg cgcacaacac gcagtcgggt gctgctgata
    10501 ggcaggcccg gtgcggtgaa cggcggggtg gcgagggcgc tacgcccccg ccaccccggc
    10561 gaggcgaccg tcctggacct cgactcccgc tatcgcccgg ccaccgaggc ggccgaggcg
    10621 gtccgcgagg cgctgcggct gtcctcgccg gacgagaacg ctccagatgc cctggacacc
    10681 ctggtactga agaagatcga ctcgctgctg cggggcaatg tcgccgccga gatcgccgca
    10741 ctcgccgagg acggggccgg tgtggtgctc gcccccgcgc tgccgatcgc cgggcgcgtg
    10801 gtgcgctcgg gcgtcgtcca cctcggcgga gtggcgctcc acgagagcga cgcatggcgc
    10861 gccgaaaccc tgcccccgcc cgcctcggtg gcccacgcgc tcggcggcct gccgaccgcg
    10921 ctggtccccc tgacga
//
