LOCUS       NZ_CP025018            20759 bp    DNA     linear   CON 12-FEB-2024
DEFINITION  Streptomyces sp. M56 chromosome.
ACCESSION   NZ_CP025018
VERSION     NZ_CP025018.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. M56
  ORGANISM  Streptomyces sp. M56
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 11742376)
  AUTHORS   Kim,K.K.Hyun., Klassen,J.L., Ruzzini,A.A.C., Beemelmanns,C.C. and
            Clardy,J.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-NOV-2017) Leibniz Institute for Natural Product
            Research and Infection Biology - Hans Knoll Institute,
            Adolf-Reichwein-Strasse 23, Jena 07745, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-AUG-2014
            Assembly Method                   :: HGAP v. AUG-2014
            Assembly Name                     :: M56v2
            Expected Final Version            :: yes
            Genome Coverage                   :: 94.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/12/2024 06:37:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,558
            CDSs (total)                      :: 9,473
            Genes (coding)                    :: 9,167
            CDSs (with protein)               :: 9,167
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 86 of 306
            Pseudo Genes (incomplete)         :: 261 of 306
            Pseudo Genes (internal stop)      :: 28 of 306
            Pseudo Genes (multiple problems)  :: 61 of 306
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:58:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2287394
            Orig. end                         :: 2308153
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP025018.1.
            Annotated using prokka 1.11 from http://www.vicbioinformatics.com.
            Bacteria and source DNA available from Christine Beemelmanns.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..20759
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hserlactone"
                     /region_number="14"
                     /rules="Autoind_synth"
                     /tool="antismash"
     cand_cluster    1..20759
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="Autoind_synth"
                     /kind="single"
                     /product="hserlactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20759
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[10000:10759](+)"
                     /cutoff="20000"
                     /detection_rule="Autoind_synth"
                     /neighbourhood="10000"
                     /product="hserlactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10759
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="Autoind_synth"
                     /neighbourhood="10000"
                     /product="hserlactone"
                     /protocluster_number="1"
     misc_feature    581..596
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 21.15"
                     /tool="antismash"
     misc_feature    985..999
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.35"
                     /tool="antismash"
     gene            1441..6081
                     /locus_tag="CFP59_RS08675"
                     /old_locus_tag="CFP59_01711"
     CDS             1441..6081
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014181661.1"
                     /locus_tag="CFP59_RS08675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_01711"
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="WP_100809092.1"
                     /transl_table=11
                     /translation="MLRRLRRRLPLPRFRHPLRFSRLFSRLSRFPRLAALALALALTVP
                     TAPAAAADPKDPPADVYKLLEDPEVTSVGQEPAHARLTPYADTAEALGGGSRSRWTASL
                     DGTWKLHMSDRPEEVPKDFFTEGYDTSEDGWRSVSVPHTWQTDGLDHPMFRNIPTEMYP
                     DDPPKVPHDVNPTGAYVKTFELPESWEKRRTFLRFEGVTSGYLVWVNGSYAGYDQGGYT
                     PAEFDISDRLHPGRNTVAVQVHRWGSGSHLEDYDQWRFSGIFRSVGLYSTPATYLRDIT
                     VKTDLDARYRDARLTAEVDIAAKGPKTPDRAPDGRKVLGTLYDQRGHKVTTMSGTVDGD
                     SGSTVLTAHVANPAKWTDETPHLYTLVVRLTDADGTVTHTTAQPVGFREIEIKDRQLLV
                     NGERILVKGVNRSETDPDTGRHATRERTASDVALMKRLNINSVRTSHYPSDPYLYELAD
                     ARGLWIDDEVDIETHHHDACPDNCLAERPEWQAAFLDRLMAMYERDKNHPSVLMWDTGN
                     EAGLGKAHYAMADFLDREDPARPVYHQPNSPDGDAPFADVWGPRYPSPSGLEEKAKATT
                     KPIIMGEYAHAMGNSLGNFREFWDVVRKYPQVQGGYIWDWAEQNITQPLLTTPDTSGND
                     ILSYVSGKPGLVAGHRGRALELSGLDDFVEVYRDPKLDAVSDALTLDAWVKPADWTGSF
                     TMIAKGDHSYALKMRDKDTLEFFVHGGGDWHTVAADVPDGWYGSWHRVSGTFDGRTLRL
                     LIDGKQTASADWTGSVDYSAQPVNIGRNPETEQENIGTRMAHGTVDQVRVYHKALSPDQ
                     LAADPSTDAVLALDFDRLDRKGEFLSYGAGTGGVDGVVSSDRTVQPEARAMAAVHAPIR
                     ISGDEARTGRVEVRNERSFTGTDDLRLRWRVTEGARTLTQGSRTLGLGPGQHTTLQLPK
                     PPANPRDADRQLTVEAVWASDTAWAKAGERVAVEQFDIGGRQLAGTVPARAPGKVRATT
                     SGGRLTVTGEGFSYGFDRTSGELVSMKAGGRELLGSGPELDAWRAPVSNEIGSEEGPWR
                     DAGLDRLRTEPGKVTVAERGGEVVVTVPSSAAAPGVRDSSFAQTLRYTVSGTGELRLDH
                     RVEARGAARAVPYLPRVGLSLRVPDRYDRFTWYGRGPQENYNDREDGAPVGVYSTDVDE
                     QFAGYTKPQDYGNHEDVRWASLSDGSSGLLVSGDFSAGVTPYTGIDRAAYPFALREDPG
                     GNNTLHLDHAVSGVSETFHTVLPEYQVRPAKEYAYTLRLRPLTGAEARTGTPRGLVVCA
                     PEAKLTPADTTVPAGGSASAELTVTNPCDTPLREVTAAFGLPEGWTATPGTLDLGDLAA
                     GRTATVQTELTRGEGTPNGPRPAVAEVRATSTGGARVSGSASAEIDGTPPPPRGEVSVS
                     TLDFLTADNGWGPVERDRSNGEDAPGDGKALTIDGTTYERGLGTHADSTVEVFLGGNCS
                     TFTATTGLDDEVGADGSVVFEVYADGERVYRGETAHGSDGAVPVEARIEGARKLRLRVT
                     DGGDGNAHDHADWAAATVRCGTGGTG"
     gene            complement(6130..6633)
                     /locus_tag="CFP59_RS08680"
                     /old_locus_tag="CFP59_01712"
     CDS             complement(6130..6633)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018539947.1"
                     /locus_tag="CFP59_RS08680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_01712"
                     /product="NUDIX domain-containing protein"
                     /protein_id="WP_069867259.1"
                     /transl_table=11
                     /translation="MTHKHSAGLLLYRGAGPAGPAVEVLLAHMGGPFWARKDEGAWTVP
                     KGEYEEDETPEAAARREFQEELGVPPPGGEPIPLGSVTQTGGKVVIVWALRGELDPAEI
                     TPGTFAMEWPKGSGRMREFPEIDRVAWFGLDEARRKIVTRQRIFLDRLEELLQEAAGAG
                     PGGA"
     gene            complement(6707..7477)
                     /locus_tag="CFP59_RS08685"
                     /old_locus_tag="CFP59_01713"
     CDS             complement(6707..7477)
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056794.1"
                     /locus_tag="CFP59_RS08685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_01713"
                     /product="methyltransferase domain-containing protein"
                     /protein_id="WP_100805442.1"
                     /transl_table=11
                     /translation="MAAAPHIELDSRSPEAVDGRQIRAVTFQDSRLEYVRGTLKHVDLP
                     APGSRAVVVGSGRGLLARGLAGLGFEVVAVDPSAAATEMAREADGGEHPGVDYRTAPAE
                     RLGLADGAFDLAYYADTFEITSELDLVLAEAARVLRPGGVLIYDTVNRTFLSRLIYLGA
                     FQRVPMTRIMPVGRYTAARLRTPAELTAALERQGLRNEDICYFKPKDPRSLVKATMARR
                     RGRISDEQIPPIVDFVLAPDARPLVTYLGYARKG"
     gene            7614..8117
                     /locus_tag="CFP59_RS08690"
                     /old_locus_tag="CFP59_01714"
     CDS             7614..8117
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1167:
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056793.1"
                     /locus_tag="CFP59_RS08690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_01714"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_100805443.1"
                     /transl_table=11
                     /translation="MTRTPLADVACSIARATDLFGDAWTALIMRDVLTGVTRFDDLAHD
                     LGISRKVLAARLTRLVEEGVLIRERYQERPPREHYRATAKGEELYPVLLALMEWGDRWY
                     AGSAGPPARIRHLGCGRDTTPVSACAHCGEPLTVGNTTQLPGPGGRTGPGTRVLGPLLA
                     AREG"
     gene            8382..8888
                     /locus_tag="CFP59_RS08695"
                     /old_locus_tag="CFP59_01715"
     CDS             8382..8888
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056792.1"
                     /locus_tag="CFP59_RS08695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_01715"
                     /product="DUF2267 domain-containing protein"
                     /protein_id="WP_100805444.1"
                     /transl_table=11
                     /translation="MRYEELTGLVQDRAQLPDRQSAERAIRATLETLAERIPGGLADHM
                     AAQLPHEAGEPLRRVAAAHEGTPEERAYRRDHGERFGLTGFAGRIAWRTERTEEEALRE
                     AAAFFEVLDSAMDPELMEKLYGVLPNDIRGLLPEARAEQDARAERDTRTERGSRTERGS
                     QAERG"
     gene            9078..9788
                     /locus_tag="CFP59_RS08700"
                     /old_locus_tag="CFP59_01716"
     CDS             9078..9788
                     /EC_number="3.5.1.88"
                     /GO_function="GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0006412 - translation [Evidence IEA]"
                     /GO_process="GO:0018206 - peptidyl-methionine modification
                     [Evidence IEA]"
                     /GO_process="GO:0031365 - N-terminal protein amino acid
                     modification [Evidence IEA]"
                     /GO_process="GO:0043686 - co-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1275: peptide deformylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056789.1"
                     /locus_tag="CFP59_RS08700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_01716"
                     /product="peptide deformylase"
                     /protein_id="WP_231981181.1"
                     /transl_table=11
                     /translation="MPTTSLSELVDELLSGIRPLPIVSAGVPVLRRPALPYDGQLAPDQ
                     LDRLLAAMRETMHSAPGVGLAAPQIGVPLRLAVIEDPAEVTAEVREARGRVPQPYRVLV
                     NPVYEPIGDGRAAFFEGCLSIPGWAAVVSRPDRIRLRGQDETGRELDEEFTGWPARIVQ
                     HETDHLGGVLYLDLAETRSLSTAQAVSDYWSQPTPATAAHALGFPLRSATGAAGAADAA
                     DAADDSGDTGATAP"
     gene            10001..10759
                     /locus_tag="CFP59_RS08705"
                     /old_locus_tag="CFP59_01717"
     CDS             10001..10759
                     /EC_number="2.3.1.-"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hserlactone: Autoind_synth"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056788.1"
                     /locus_tag="CFP59_RS08705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_01717"
                     /product="GNAT family N-acyltransferase"
                     /protein_id="WP_069867263.1"
                     /sec_met_domain="Autoind_synth (E-value: 0.0001, bitscore:
                     21.0, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSVLTTSSTTAATSSYVTSIAHTPEQIHAAQRLRYQVFGEERGGR
                     LDAAVDGRDVDEFDEVMDHLIVTDTATGTVVGTYRLLPPGRSERLYSDTEFDLRGLPAE
                     VRSSMVEAGRACVHADHRSGAVINLMWAGLARYVLLSGHRYLAGCASVPLADGEQAAAG
                     AWLLGTTKHAAPPEMRVHPHDPWIPSRPLDGEPSYAELPPLLRGYLRVGAWMCGSPQHD
                     RAFNDADFFVLLDTERMNDRYRRYFLGESQ"
     gene            10756..11613
                     /locus_tag="CFP59_RS08710"
                     /old_locus_tag="CFP59_01718"
     CDS             10756..11613
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1141: acyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056787.1"
                     /locus_tag="CFP59_RS08710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_01718"
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="WP_100805445.1"
                     /transl_table=11
                     /translation="MTVPAAMSLPAHAWAAWSPCTTGCVAHAAGRVPATRAARRAAVFG
                     RSVMGALLSGQKIAEPERLRARAAALLGSLGIRLEVTGDTGLSAPGGTGTLVVANHISW
                     LDVIALLAIEPVVMLAKREIADWPLIGPMVTRAGTMYIDRGSLRELPETVREMAARLRD
                     GQSVMAFPQGITWCAGTGGSFRRATFQAALDAGAPVRPVTLDYVQHGAPTTVAAFVGED
                     DFGASLRRVLRADGLTVRVDLHRPLRPMRAVDDRRRVAAQAQATVCGARLPLHHEVAAL
                     ELAR"
     gene            complement(11918..13225)
                     /gene="paaK"
                     /locus_tag="CFP59_RS08715"
                     /old_locus_tag="CFP59_01719"
     CDS             complement(11918..13225)
                     /EC_number="6.2.1.30"
                     /GO_function="GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA]"
                     /GO_process="GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: AMP-binding (87-338). E-value: 4.7e-21.
                     Score: 66.7. Matches aSDomain:
                     nrpspksdomains_CFP59_RS08715_AMP-binding.1"
                     /codon_start=1
                     /gene="paaK"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056786.1"
                     /locus_tag="CFP59_RS08715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_01719"
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="WP_100805446.1"
                     /sec_met_domain="AMP-binding (E-value: 1.3e-25, bitscore:
                     88.9, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAGSPSAAPLDDAERMSPEELRALQLTRLRATLRHAYDHVELYRK
                     KFREAGVGPEDCRTLEDLARFPFTTKADLRDSYPFGMFAVEQSEVRRIHASSGTTGRPT
                     LVGYTEHDLAVWAEVIARSIRAAGGRPGHKVHVSYGYGLFTGGLGAHYGAERLGCTVIP
                     ASGGMTARQVRLIQDLRPEIIMITPSYLLTLLDEFERQGVDPRSTSLQVGIFGAEPWTE
                     EMRREIEERMGIHAVDIYGLSEVIGPGVAQECVETKDGLHVWEDHFYPEIVDPLTDAVL
                     PGGEEGELTFTTLTKEALPVIRYRTRDLTRLLPGTARPAFRRIQKITGRCDDMLIVRGV
                     NVFPSQIEEIVLRVPAVAPHFQVELTRRGRMDHMAVRIEARPGAEPEQRAAAAAAIVRG
                     VKDGVGLTIEVEVVDPETLERSVGKIRQVKDLRTEG"
     CDS_motif       complement(12182..12244)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CFP59_RS08715_0001"
                     /evalue="3.00E-04"
                     /label="NRPS-A_a8"
                     /locus_tag="CFP59_RS08715"
                     /protein_end="348"
                     /protein_start="327"
                     /score="13.6"
                     /tool="antismash"
                     /translation="TGRCDDMLIVRGVNVFPSQIE"
     aSDomain        complement(12212..12964)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CFP59_RS08715_AMP-binding.1"
                     /evalue="4.70E-21"
                     /label="CFP59_RS08715_AMP-binding.1"
                     /locus_tag="CFP59_RS08715"
                     /protein_end="338"
                     /protein_start="87"
                     /score="66.7"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="SEVRRIHASSGTTGRPTLVGYTEHDLAVWAEVIARSIRAAGGRPG
                     HKVHVSYGYGLFTGGLGAHYGAERLGCTVIPASGGMTARQVRLIQDLRPEIIMITPSYL
                     LTLLDEFERQGVDPRSTSLQVGIFGAEPWTEEMRREIEERMGIHAVDIYGLSEVIGPGV
                     AQECVETKDGLHVWEDHFYPEIVDPLTDAVLPGGEEGELTFTTLTKEALPVIRYRTRDL
                     TRLLPGTARPAFRRIQKITGRCDDMLIVR"
     gene            13453..13980
                     /locus_tag="CFP59_RS08720"
                     /old_locus_tag="CFP59_01720"
     CDS             13453..13980
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CFP59_RS08720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="CFP59_01720"
                     /product="hypothetical protein"
                     /protein_id="WP_100805447.1"
                     /transl_table=11
                     /translation="MYDAADADVPHPVRIADRAPRRRGPRTVAVAAAVGALLALAGCGG
                     GGGGGGKADAKAETGGDATRPSASPAPKAARVEQLASAAGCKPEFTTKVDDYRQAVCKN
                     SKGKFVFLDFVTAKGQRDWLETAQMYGGVYLVGNRWVLSSSPRKNMERLRDEFGGTIEG
                     GTSYGSASGTPQ"
     gene            complement(14248..15024)
                     /locus_tag="CFP59_RS08725"
                     /old_locus_tag="CFP59_01721"
     CDS             complement(14248..15024)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056784.1"
                     /locus_tag="CFP59_RS08725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_01721"
                     /product="TIM barrel protein"
                     /protein_id="WP_100805448.1"
                     /transl_table=11
                     /translation="MTRLGICSVTLRRLDPDRVIAAVAGAGLECVEWGGDVHVPPGDED
                     TARRVRDATVDAGLAVASYGSYYRAGHGDPEEFDAVLRSAVALGAPRVRIWAGATGTRE
                     TSPAGRRAVVEDTRRAAALAADAGVELAYEFHRNTLTDGADHTLRLLAEVDRAEVATYW
                     QPPVDMPDAEALKGLEALGDRIAAVHAFSWWPGTTRLPLTARAPLWRGAFERLRAHRAT
                     GSGGGPPLDVLLEFVPGDDERLLPREAATLRALADV"
     gene            complement(15021..16976)
                     /locus_tag="CFP59_RS08730"
                     /old_locus_tag="CFP59_01722"
     CDS             complement(15021..16976)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056783.1"
                     /locus_tag="CFP59_RS08730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_01722"
                     /product="DUF2264 domain-containing protein"
                     /protein_id="WP_100805449.1"
                     /transl_table=11
                     /translation="MPELPFLLPADDRALSPHTGYTRAHWEAAADGMLHAALRYATPGQ
                     ALIDLPGPPSKSGVRSDGLEGFARTFLLAAFRAAGASGQDQHGILERYTAGIARGTLTP
                     GRGDAESWPVIGHHGAHGQPMVESASVALGLRLTAPWTWDALPPDAQDRTEEWLRGALR
                     HQPAPNNWYLFPLTVAGFLESAGRGDAETARAIERGLALLEGWYQGQGWYSDGDGRSFD
                     HYNGWALHMYPLLHAHLAADGALLDRLGPRLRTFLESFSLLFDADGAPIHQGRSLTYRF
                     AAGASVALGALTGHTPLAPGATRRVLSGALRHFLDRGALTEDGVLSLGWYGPHAATLQR
                     YSGPGSPYWASKGFLALLLPPGHPVWTEPEEAAPAEGPDRALALPATGLLIQTTGRDGL
                     VRLHNHGSYKLRPWEAEYAPADPLYARLAYSTRTGPTSADNAPDNHIALEVRGVRSDRL
                     RIHPLAAGPDWLASSHTPAFPDGGPKVPALRIDSLTLVRGRLEVRVHRTVGVPAGTRIH
                     HSGWAVALPPGTPAETERGAERGAEVRLDGGAVTGQLLGLHGWETATAVRAPQGTAYGP
                     WALVPELTGTVGENPSGTLFVALASLTGERDPAPLADLATAEVHGLAVTVRLPDGADVV
                     VDFGTGDAPTVTEVSA"
     gene            complement(17192..18235)
                     /locus_tag="CFP59_RS08735"
                     /old_locus_tag="CFP59_01723"
     CDS             complement(17192..18235)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2-Hacid_dh_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1072: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056782.1"
                     /locus_tag="CFP59_RS08735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_01723"
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="WP_100805450.1"
                     /sec_met_domain="2-Hacid_dh_C (E-value: 5e-46, bitscore:
                     155.4, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAPQISARTRTRTRPRAVLAMAERSARQVLGSVSPDRLTELLDL
                     DPALVLDDFTTPAAHRALAEAELLVTGWGCPPLDRRALDAAPRLRAVVHTAGTVRHHIT
                     DACWERGISVSSAAAANALPVAEYTVAMILLANKRVLDIARDYRAERRAIDWNERYPDA
                     GNYRRTVGILSASAIGRRVIELLRPYDLDVLLYDPYVTAREAEELGTRRVPLRELFARS
                     DVISVHTPLLPATEGLVGRELLAAMPDGATLINTARGAVVDQAALTEEVVAGRIRAVLD
                     VTVPEVLPADSPLYDCDNALITPHIAGSKSGELRRLADLAIGEIENYVTGRAFAHPVRP
                     EILDRSA"
     gene            complement(18232..19146)
                     /locus_tag="CFP59_RS08740"
                     /old_locus_tag="CFP59_01724"
     CDS             complement(18232..19146)
                     /GO_process="GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1069: putative ABC
                     transporter permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014181530.1"
                     /locus_tag="CFP59_RS08740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_01724"
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="WP_161370488.1"
                     /transl_table=11
                     /translation="MTTTTPTSPVGASRGPSSRPLGKPFGGSRLTSRGVANAVVLIAAL
                     YTMLPALWLLLASTKNADALFGSDIFSLGDFSFARNLSDLFSMDGGLYGEWYVNSLLYA
                     VVGALTGSLISVAAGYAFDKYEFRHKEKFFGLVLTGVMVPPTVLALPLYLVASEIGMVN
                     TFWSVFVPVLFNPFGVYLARLLSSGYVPNEVLEASRVDGAGELQTYVRVSLRMLGPGFV
                     TVFLFQLTAIWNNFFLPMVMLSDQHLYPLSLGLYTWNSAATVSPEYYPLVVIGSLLAVV
                     PLIVAFVLLQRYWKSGLTAGSVK"
     gene            complement(19146..20105)
                     /locus_tag="CFP59_RS08745"
                     /old_locus_tag="CFP59_01725"
     CDS             complement(19146..20105)
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1067: putative ABC
                     transporter permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009712831.1"
                     /locus_tag="CFP59_RS08745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_01725"
                     /product="sugar ABC transporter permease"
                     /protein_id="WP_100805451.1"
                     /transl_table=11
                     /translation="MSTVTSSKGTAPPSAAAADPTRRTGRRQQRIAAAVLLTPFTALLV
                     AVFLIPVGYAVYLSFFSEDHEGLGFGGGRTVFTGLRSYLSVLEDPSFLSGFGTIALYCL
                     IFVPLVVISALALGLLLDSGLVRLRRTAQMLLYLPHAVPGIIAAVIWLYLYTPGLSPVI
                     KLFARADITVDFLGLHTVLPSIVNIALWSGLGYNMVIFYAALQALPREVIEAATIDGAG
                     GIRTALQVKVPIIRGSVVMVCMFSLIGALQLFTEPMLMNQATPMVNSRFTPNMYIYDAA
                     FRRNNYGLASAASVILLIVTCVLSYAVTRWSGRRERRA"
     misc_feature    complement(20091..20106)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.38"
                     /tool="antismash"
     misc_feature    complement(20304..20319)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.85"
                     /tool="antismash"
     misc_feature    complement(20513..20527)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.69"
                     /tool="antismash"
ORIGIN
        1 cgacccgcag cagccgttcg gagccctccg gatgctggac gaagcgcagg gtggactgga
       61 gcgtcatccc ggcggcgaag agccgctgga tggccaggtc gtgcagatcg cgggcgatcc
      121 ggtcgcggtc ctcgagcagg gcgagctgtt cggcgtcgcg ccgccgctcg gccagtttca
      181 tcgcgatcgc cgcctgaccg gcgaacccca gcagggtgtc ggtctcgacc tgggtgaagg
      241 cgtggctgtc cgccacacgg gccagcgaga gcaccccgcc cactccgtcg tgcgtccgca
      301 tcggcaccgc gacggccggg ccgagcccct cccagcgctg cgggccgacc gtgatccgcg
      361 ggtcgttctg gacatcggtg ctggtgaccg gctcggaggc ggtgaaggcc gccccgccga
      421 aggtgccccg gcggggcagt acaagaccgc ggtgggcctc ggcgtcggtc cccgacgcca
      481 gcgcgacctg gagttcgtcg gtgtcggcca ccggcagcat cagcactccg agatccgcgg
      541 agaggatccg ccgcgcgtgc tcgacgatca gctccaggac cctggtctcg tccactccgg
      601 acagcaggct gtgggtgacc tcggcgttcg cctccagcca gcgctggcgg tgccgggcct
      661 cctcgtacag ccgggcgttc tcgatggcca ccccggcggc cacggccagg gtggacagca
      721 ccgtctcgtc ctcgccgtcg aactcctcac cgccgcgctt ctcggtgagg tagaggttgc
      781 cgaagacctc gtcgcgcacc cggatcggca ccccgaggaa ggagcgcatc ggcggatggt
      841 tcggcggaaa gccgtaggag gcgggatgtt ccgagagctc acccagccgc agtggcttgg
      901 ggtggcgaat cagctcgccc aggagaccgt ggcccgtcgg cagccgcccg atcgcctcgg
      961 cccgctcctt gtcgacgccc accgggagga attcggccag ccgctgatcc tcgccgatca
     1021 cccccagcgc cccgtattcg gcgtccacca gcaccacggc ggcctcgacg atcctgcgca
     1081 gcacatgcga gagatcgagc tcccggccga ccgtgaggac cgcttccagc agactgtgga
     1141 cccggtcctg ggtgccgcgc accccgtcga tccgcacctg caactcttcc agcagctcat
     1201 ccagccgcag ccggggcgga tactccgtac tcctggcgct ctccataccc acggtgcctc
     1261 cgtcggcctc accgcgcgct cggcacggca ttccgcacgg ccctcactct cttcacggta
     1321 gccgcgccgg gccgagggtg agtgccgcgc gacatcttgg cgcgaggggg cgcgttggct
     1381 aatgtgcgga cctgttcgct ctggaacgtt tcaacacccc atagaagggt ggaccaccag
     1441 atgctccgac gcctccgccg tcgtctacct ctcccccgtt tccgccaccc cctccgcttc
     1501 tcccgccttt tctcccgcct ttcgcgcttc ccccggctcg ccgccctcgc cctggccctc
     1561 gcgctgaccg tccccaccgc cccggcggca gcggcggatc ccaaggatcc gcccgcggat
     1621 gtgtacaagc tgctggagga ccccgaggtc acctcggtcg ggcaggagcc cgcccacgcc
     1681 cggctgaccc cgtacgccga caccgccgaa gccctcggcg gcggctccag gagccgctgg
     1741 accgcctcgc tcgacggcac ctggaagctc catatgtccg accgccccga ggaggtgccc
     1801 aaggacttct tcaccgaggg ctatgacacc tccgaggacg gctggcgcag cgtgagcgtg
     1861 ccgcacacct ggcagaccga cggtctcgac catccgatgt tccggaacat ccccaccgag
     1921 atgtatccgg acgatccccc gaaggtcccg cacgacgtca atccgaccgg cgcctacgtc
     1981 aagaccttcg agctgccgga gagctgggag aagcggcgga cattcctccg cttcgagggc
     2041 gtcaccagcg gctatctggt gtgggtgaac ggctcctacg cgggctatga ccagggcggc
     2101 tacacccccg ccgagttcga catcagcgac cggctgcacc cggggcgcaa caccgtcgcg
     2161 gtgcaggtcc accgctgggg ttcggggtcg catctggagg actacgacca gtggcggttc
     2221 tcgggcatct tccgctcggt cgggctgtac tccaccccgg ccacctatct gcgggacatc
     2281 acggtcaaga ccgatctcga cgcccggtac cgcgacgccc ggctgaccgc cgaggtcgac
     2341 atcgccgcga aggggccgaa gacaccggac cgcgccccgg acgggcgcaa ggtcctcgga
     2401 accctgtacg accagcgcgg gcacaaggtg acaacgatgt cggggacggt ggacggcgac
     2461 agcgggtcca ccgttctcac cgcccacgtg gccaacccgg ccaaatggac cgatgagacg
     2521 ccccacctct acaccctcgt cgtccggctc accgacgccg acggcacggt cacccacacc
     2581 acggcccagc cggtgggctt ccgcgagatc gagatcaagg acagacagct cctcgtcaac
     2641 ggcgagcgca tcctcgtcaa gggcgtcaac cgctccgaga ccgaccccga caccggccgc
     2701 cacgccaccc gtgagcgcac cgcctcggat gtcgccctga tgaagcggct caacatcaac
     2761 tccgtgcgca cctcgcacta cccctcggat ccgtacctct acgaactggc cgacgcacgc
     2821 ggtctgtgga tcgacgacga ggtggacatc gagacccatc accacgacgc ctgcccggac
     2881 aactgcctgg ccgaacggcc cgagtggcag gcggcgttcc tggaccggct catggcgatg
     2941 tacgaacggg acaagaacca ccccagcgtg ctgatgtggg acaccggcaa cgaggccgga
     3001 ctgggcaagg cccactacgc gatggccgac ttcctggacc gcgaggatcc ggcccggccg
     3061 gtctaccacc agcccaacag cccggacggc gacgccccgt tcgccgatgt ctggggcccg
     3121 cgctacccct ccccctcggg cctggaggag aaggcgaagg ccaccaccaa gccgatcatc
     3181 atgggcgagt acgcccacgc catgggcaac tcgctcggca acttccggga gttctgggac
     3241 gtcgtgcgga agtaccccca ggtccagggc ggctacatct gggactgggc ggagcagaac
     3301 atcacccagc cgctgctcac cacccccgac acctccggca acgacatcct ctcctatgtc
     3361 tcgggaaagc ccggtctggt ggccggccac cggggcaggg cgctggagct gtccggtctt
     3421 gacgacttcg tggaggtcta ccgcgatccg aagctggacg cggtgtcgga cgcgctcacc
     3481 ctggacgcct gggtgaaacc ggccgactgg accggctcct tcacgatgat cgccaagggc
     3541 gaccacagct atgccctgaa gatgcgcgac aaggacaccc tggagttctt cgtccacggg
     3601 ggcggggact ggcacaccgt cgcggccgat gtccccgacg gctggtacgg ctcgtggcat
     3661 cgcgtctcgg gcaccttcga cggccgtacg ctccggctgc tcatcgacgg gaaacagacc
     3721 gcgtcggccg actggaccgg cagcgtggac tactccgcgc agccggtgaa catcggccgc
     3781 aacccggaga cggagcagga gaacatcggc acccggatgg cacacggcac cgtcgaccag
     3841 gtccgcgtct accacaaggc gctgagcccc gatcagctcg ccgccgaccc cagcacggac
     3901 gcggtgctcg cgctcgactt cgaccggctc gaccgcaagg gggagttcct gtcctatggc
     3961 gccgggaccg gcggggtcga cggcgtggtc tcctccgacc gcaccgtcca gcccgaggcc
     4021 cgcgccatgg cggccgtcca cgccccgatc cggatctcgg gcgacgaggc ccgcacgggc
     4081 cgggtcgagg tgcgcaacga gcggtccttc accggcaccg acgacctccg gctgcgctgg
     4141 cgcgtcaccg agggcgcccg caccctgacc cagggcagcc gcacgctcgg cctgggcccc
     4201 ggtcagcaca ccaccctcca gctgccgaag ccgcccgcca acccccggga cgccgaccgg
     4261 cagctgaccg tggaggcggt gtgggcgtcc gacacggcct gggcgaaggc gggggaacgg
     4321 gtggcggtgg agcagttcga catcggcggc cggcagctcg cgggcacggt gccggcgcgg
     4381 gcaccgggga aggtgcgggc caccacgtcc ggcggccgcc tcaccgtcac cggcgagggc
     4441 ttctcgtacg gcttcgacag gaccagtggc gaactggtct ccatgaaggc cggtggccgg
     4501 gagctgctcg gctccggccc cgagctggac gcctggcgcg ccccggtcag caatgagatc
     4561 ggctccgagg aggggccctg gcgcgatgcc ggcctggacc ggctgcgcac cgaaccgggc
     4621 aaggtcacgg tggcggagcg gggcggcgag gtcgtcgtga ccgtcccctc atcggccgcc
     4681 gcgcccggtg tgcgggactc ctcgttcgcc cagaccctgc ggtacaccgt cagcggcacc
     4741 ggggagctgc gcctcgacca ccgcgtagag gcccggggcg cggcgcgcgc ggtgccctat
     4801 ctgccgcgcg tcgggctctc gctccgggtg cccgaccgct acgaccggtt cacctggtac
     4861 gggcgcgggc cgcaggagaa ctacaacgac cgcgaggacg gtgccccggt gggggtgtac
     4921 tccacggacg tggacgagca gttcgccgga tacaccaagc cgcaggacta cgggaaccac
     4981 gaggacgtcc gctgggcctc gctgtccgac gggagtagcg ggctcctggt gtccggggac
     5041 ttctcggcgg gcgtgacccc gtacaccggg atcgaccgcg ccgcctaccc gttcgccctc
     5101 cgcgaggatc cgggaggcaa caacaccctg cacctggacc acgcggtgag cggggtgagc
     5161 gagacgttcc acaccgtgct gcccgagtat caggtgcggc ccgccaagga gtacgcctac
     5221 accctgcggc tgcgcccgct gaccggcgcc gaggcccgca ccggcacccc gcgcggcctg
     5281 gtggtctgcg cccccgaggc gaaactcacc ccagccgaca ccacggtgcc ggcgggcggc
     5341 tccgcctcgg ccgagctgac cgtcaccaac ccctgcgaca ccccgctgcg cgaggtcacc
     5401 gccgccttcg gtctgcccga ggggtggacc gccacgcccg gcaccctcga cctgggcgat
     5461 ctcgcggcgg gccggaccgc caccgtacag accgagctca cccgtggtga gggcaccccg
     5521 aacggcccgc ggcccgccgt ggccgaggtc cgcgcgacct cgaccggtgg cgcgcgggtg
     5581 agcggctcgg cgtcggccga gatcgacggc acgccgcccc cgccacgcgg tgaagtgtcc
     5641 gtgtccacgc tcgacttcct caccgcggac aacggctggg gcccggtcga acgcgaccgc
     5701 agcaacggcg aggacgcccc gggcgacggc aaggcgctca ccatcgacgg cacgacctac
     5761 gagcggggcc tcggcacaca cgccgattcg acggtcgagg tcttcctggg cggcaactgc
     5821 tcgacgttca ccgcgaccac gggcctggac gacgaggtgg gcgccgacgg cagcgtcgtc
     5881 ttcgaggtct acgccgacgg cgagcgggtg taccggggcg aaacggcaca cggctcggac
     5941 ggggccgtac cggtggaggc gcgcatcgag ggcgcgcgga agctgcggct gcgggtcacc
     6001 gacggcggcg acggcaacgc gcatgaccac gccgactggg cggcggccac ggtgcgctgc
     6061 ggaacgggag gtacggggtg agctgacggc ggcggggggt gagctgacgg cggcgggcgc
     6121 gggccacggt catgcgccgc cggggcccgc gcccgccgcc tcctgaagca gctcttcgag
     6181 ccggtccagg aagatccgct gcctcgtcac gatcttccgg cgggcctcgt ccagaccgaa
     6241 ccaggcgacc cggtcgatct cggggaactc ccgcatccgg cccgagccct tcggccactc
     6301 catggcgaac gtaccgggag tgatctccgc cggatccagc tcgcccctga gcgcccagac
     6361 gatgaccacc ttgcccccgg tctgtgtgac ggagccgagc ggaatcggct caccgccggg
     6421 cgggggcact cccagctcct cctggaactc ccggcgcgcg gcggcctccg gggtctcgtc
     6481 ctcctcgtac tcccccttgg gcaccgtcca ggcgccctcg tccttcctgg cccagaacgg
     6541 gccgcccata tgggcgagca gcacctcgac ggcgggaccg gccgggcccg caccgcggta
     6601 cagcaggagt ccggcgctgt gcttgtgcgt cacagcgccc agtgtgcgcc ccgggccgcc
     6661 ggggcggggt gccgggcacg ccggggcggg gtgccgggca ccccgctcag cccttgcggg
     6721 cgtagccgag ataggtgacc agcggccggg cgtccggggc caggacgaag tcgacgatcg
     6781 gcgggatctg ctcatcgctg atccgccccc ggcggcgcgc catggtggcc ttgaccaggc
     6841 tgcgcggatc cttgggcttg aagtagcaga tgtcctcgtt gcgcagcccc tgccgctcca
     6901 gggcggcggt cagctcggcg ggggtgcgca gccgggccgc ggtgtagcgg ccgaccggca
     6961 tgatccgggt catcgggacg cgctggaagg cgccgaggta gatcagccgg gagagaaagg
     7021 tgcggttgac ggtgtcgtag atcagcacac cgccgggccg cagcacccgg gcggcctccg
     7081 cgagcaccag gtcgagctcc gaggtgatct cgaaggtgtc cgcgtagtag gcgaggtcga
     7141 acgcgccgtc ggcgaggccg agtcgctcgg cgggcgcggt gcggtagtcg accccggggt
     7201 gctcgccccc gtccgcctcg cgcgccatct cggtcgcggc ggcggaggga tcgacggcca
     7261 cgacctcgaa gccgagaccg gccagccctc gggccagcag cccgcggccg ctgcccacga
     7321 ccacggcgcg gcttccgggc gcggggaggt ccacgtgctt gagcgtgccg cggacgtact
     7381 ccaggcggct gtcctggaag gtgacggccc tgatctggcg gccgtcgacg gcctcgggcg
     7441 accgtgagtc cagttcgatg tgcggggcgg cggccatcgt cttctccctc acgggtgcgc
     7501 ggattcgcgg attcaccggc gtgcccgggg tggtccggac gggctcgcct cgggttcccc
     7561 gaggaaacct aacatcggtt agttccctcg gggaactcgg gtagggtcgt gtcatgaccc
     7621 gtactccgct cgccgatgtg gcctgttcga tcgcccgtgc caccgatctg ttcggcgacg
     7681 cctggacggc gctgatcatg cgggatgtgc tcaccggggt cacccgcttc gacgatctcg
     7741 cccatgacct gggcatatcg cgcaaggtcc tggccgcccg gctgacccgg ctggtcgagg
     7801 agggggtgct gatccgggag cgataccagg agcggccgcc gcgtgagcac tatcgcgcca
     7861 ccgcgaaggg cgaggagctc tatccggtgc tgctcgcgct gatggagtgg ggcgaccgct
     7921 ggtacgccgg ctccgccgga cccccggccc ggatccgcca cctcggctgc ggacgggaca
     7981 ccaccccggt ctccgcgtgc gcccactgcg gggagcccct gacggtcggc aacaccaccc
     8041 agctcccggg ccccggcggg cgcaccggac ccggcacccg ggtgctgggc ccgctgctcg
     8101 ccgcgcgcga gggctgaggg cttcgccgag ccgaccgtgt cgatgtgcgg ctccgccgcg
     8161 tgggcgcggc cgcgagcggc cgacggcacc ccggcgcact tcgcccgagc cccctcaggg
     8221 cacctttcga accgcggccg accgccaccg atccgctgag cggacggcac ccgcctggac
     8281 ccggtcccgg cgcgcgccac ggttcgggcg aacggcaggg ggcactcgac gcggggcact
     8341 cggcacagca gccgggcatc cgaccacgag gagcgccgac catgcgctac gaagaactga
     8401 cgggcctggt ccaggaccgg gcccagctgc ccgaccggca gtcggccgag cgggcgatac
     8461 gggcgacgct cgaaaccctc gccgagcgca tcccgggcgg gctggcggac cacatggcgg
     8521 cccagttgcc gcatgaggcg ggcgagcccc tgcgccgggt cgcggccgcg cacgagggca
     8581 cgcccgagga gcgggcgtac cggcgggacc acggcgagcg gttcggcctc accggtttcg
     8641 cgggccggat cgcctggcgt accgagcgca ccgaggagga ggcgctgcgc gaggcggccg
     8701 cgttcttcga ggtgctggac tcggccatgg acccggagct gatggagaag ctctacggcg
     8761 tactgccgaa cgacatccgc ggactgctgc cggaggcacg ggcagaacag gacgcacggg
     8821 ccgagcggga cacacgaacc gaacggggct cgcggaccga acggggctcc caggccgagc
     8881 ggggctgagc accccgctgg aagtgccgtg acgtgccgtc gatcgtccat ggcaccctcg
     8941 tggccggtcg cgcccgcgcg gcggagccgc acaccgacac cgccccgcgc cccttcgggg
     9001 cgccaccgaa ccgcagccga cttccgctcc cacctccgcg aattgccgtg gcgtgatcgt
     9061 cttgccagtc tgtccccatg ccgaccacca gcctctccga gctcgtcgat gaactgctct
     9121 ccgggatccg cccgctgccg atcgtctccg cgggcgtccc cgtgctccgg cgaccggccc
     9181 tgccctacga cggccaactg gccccggacc agctggaccg gctgctggcg gcgatgcggg
     9241 agacgatgca ctccgcgccc ggagtggggc tcgccgcccc gcagatcggt gtgccgctgc
     9301 ggctcgcggt gatcgaggac ccggcggagg tgacggccga ggtgcgggag gcgcgcggcc
     9361 gggtgccgca gccgtaccga gtgctggtca atcccgtcta cgaaccgatc ggcgacggcc
     9421 gtgcggcgtt cttcgagggg tgtctgagca tccctggatg ggccgccgtg gtcagccgcc
     9481 cggaccggat ccggctgcgt gggcaggacg agacggggcg ggagctggac gaggagttca
     9541 ccggctggcc cgcgcggatc gtccagcacg agacagacca tctcggcggc gtcctctatc
     9601 tggatctggc cgaaactcgc tccctgtcca cggcccaggc cgtgagcgac tactggtccc
     9661 agcccactcc tgccaccgcg gcccacgcgc tgggcttccc gctccggtcc gccaccggcg
     9721 ccgccggcgc cgccgacgcc gccgacgccg ccgatgacag cggcgacacc ggcgctaccg
     9781 ctccgtaacc acgccctacc agcggcatgc tttcgtcatc gactggcagt cgtcgaatca
     9841 ccgcacggac actgtgacgt acgtcgccac ggcaagactg gtggatgccc gcggtacgcg
     9901 ccgcctcagc gcaccgcacg gcgagtgagg cgtttcttcc gctcttccgc accctcccca
     9961 gtccccaccg ctcctgactt cccccgaacg gagaacagga atgtccgtgc tcacgacgtc
    10021 ctcgacaacg gcggccacca gctcgtacgt cacctcgatc gcccacaccc cggagcagat
    10081 ccacgcggcg cagcggctgc gctaccaggt gttcggcgaa gagaggggcg gtcggctgga
    10141 cgcggcggtc gacggccgcg atgtggatga gttcgacgag gtcatggacc acctgatcgt
    10201 cacggacacg gcgacgggca ccgtggtcgg cacctaccga ctgcttcccc cggggcgcag
    10261 tgagcggctc tactcggaca ccgagttcga tctgcgcggg cttccggccg aggtccgctc
    10321 gtccatggtc gaggccggcc gcgcctgtgt acacgccgac caccgctcgg gcgccgtcat
    10381 caacctcatg tgggccggtc tggcccgcta tgtgctgctg tccggacacc gctatctcgc
    10441 gggctgcgcc tcggttccgc tggccgacgg cgagcaggcc gccgcgggcg cctggctgct
    10501 cggcaccacc aagcacgccg ccccgcccga gatgcgggtc cacccgcacg acccctggat
    10561 accctcgcgg ccgctggacg gcgagcccag ctacgccgaa ctgccgccgc tgctccgtgg
    10621 ctatctgcgg gtcggtgcct ggatgtgcgg atccccgcag cacgaccggg ccttcaacga
    10681 cgccgacttc ttcgtgctgt tggacaccga gcggatgaac gaccgctacc gccgctactt
    10741 cctgggtgaa tcccagtgac cgtcccggcc gcgatgagcc tgcccgcgca cgcctgggcg
    10801 gcctggtccc cgtgcaccac cggctgtgtc gcccacgccg cgggccgcgt cccggccacc
    10861 cgggccgccc ggcgggccgc ggtcttcggc cggtcggtga tgggcgcgct gctgtcgggg
    10921 cagaagatcg ccgagcccga gaggctgcgt gcgcgcgcgg ccgcgctgct gggctccctc
    10981 gggatccggc tggaggtgac cggcgacacc gggctcagcg ccccgggcgg caccggcacg
    11041 ctggtcgtgg cgaaccacat ctcctggctc gacgtcatcg cgctgctcgc gatagagccc
    11101 gtggtcatgc tggccaagcg ggagatcgcg gactggccgc tgatcggccc gatggtcacc
    11161 cgcgcgggca cgatgtacat cgaccgcggc tcgctgcgcg aactccccga aaccgtacgg
    11221 gagatggcgg ccaggctgcg cgacggccag tcggtgatgg cctttccgca gggcatcacc
    11281 tggtgtgcgg gcacgggcgg cagcttccgg cgggccacct tccaggccgc gctggacgcg
    11341 ggggcgccgg tgcgcccggt gaccctggac tacgtccagc acggcgcccc gaccacggtg
    11401 gccgccttcg tcggcgagga cgacttcggc gcctcgctgc gccgggtgct ccgcgccgac
    11461 gggctcacgg tgcgggtcga cctgcaccgg ccgctgcggc cgatgcgggc cgtcgacgac
    11521 cggcgcaggg tcgcggcgca ggcccaggcg accgtctgcg gggcgcggtt gccgctccat
    11581 cacgaggtgg ccgccctcga gctggcacgc tgagccaatc gcctccgggc gcgccgtcct
    11641 gtctccacgg atggcgcccg gggcggtgct cagcaactcg tacgcgcgcc ggttcagctt
    11701 tccgtccgca ggtcccgggg tgggattcgg ccgcccgtac gcagggtgct cgcccctcag
    11761 cccgaggtcc tgggacagtg ctcaatgccc cctacgcagg agggccctca gccctcagcc
    11821 cgcggtcccg gggcggtgct caatacccca tacgcagggc cctcagccct ccgtccgagg
    11881 tcccggggcg gtgctcaata ccccatacgc agggccctca gccctccgtc cgcaggtcct
    11941 tgacctggcg gatcttcccc acggagcgct ccagcgtctc cggatccacg acctccacct
    12001 cgatcgtgag ccccacaccg tccttgactc cccggacgat cgccgccgcg gcggccgcgc
    12061 gctgctcggg ctcggccccg gggcgcgcct cgatccgtac cgccatatgg tccatccggc
    12121 cgcgccgggt cagctcgacc tggaagtgcg gcgccaccgc cgggacccgc agcacgatct
    12181 cctcgatctg gctggggaag acattgaccc cgcgcacgat gagcatgtcg tcacagcggc
    12241 cggtgatctt ctggatgcgc cggaaggcgg ggcgggcggt gccgggcagc agccgggtca
    12301 gatcccgggt gcggtagcgg atgacgggga gcgcctcctt ggtgagggtg gtgaaggtca
    12361 gctccccctc ctcgccgccg ggcagcaccg cgtcggtcag cgggtccacg atctccgggt
    12421 agaagtggtc ctcccagacg tgcagcccgt ccttggtctc cacgcactcc tgggccaccc
    12481 ccgggccgat cacctccgag agcccgtaga tgtccaccgc gtggatgccc atgcgctcct
    12541 cgatctcccg gcgcatctcc tccgtccagg gttcggcgcc gaatatgccg acctggaggg
    12601 aggtggagcg gggatcgacg ccctgtcgct cgaactcgtc gaggagcgtc agcagatacg
    12661 agggcgtgat catgatgatc tcgggcctga ggtcctggat gagccgcacc tgccgggcgg
    12721 tcattccgcc ggaggcgggg atgacggtac agccgagccg ctcggcgccg tagtgggcgc
    12781 cgaggccgcc ggtgaacagg ccgtagccgt aggagacatg caccttgtgg ccgggccgac
    12841 cgccggcggc gcggatcgag cgggcgatca cctccgccca taccgccagg tcgtgctcgg
    12901 tgtagccgac gagggtggga cggccggtgg taccgctgga ggcgtggatc cggcgcacct
    12961 cgctctgctc gacggcgaac atcccgaagg ggtagctgtc gcgcagatcg gccttggtgg
    13021 tgaaggggaa ccgggccagg tcctccaggg tgcggcagtc ctcggggccg accccggcct
    13081 cgcggaactt cttgcggtac agctcgacat ggtcgtacgc atggcgcagg gtggcgcgca
    13141 gccgggtgag ctgaagcgcc ctcagctcct cagggctcat ccgctcggcg tcgtcgagcg
    13201 gagccgcgct cggggatccc gccatgggcc gtccaccacc tccgattgcc gaccgaacat
    13261 tcggttaccg atcgggcgcg gtccagtaat ccagcatccg acctggctgt caagggttgg
    13321 gacactcccc tgcgcacccg gccgggcccg gggcggcgcg tggacggtga acggtcggct
    13381 cgacccatcc gtatccgtgg gtgcggtgga ctcagccgcc cgcacccagg atccgcgacg
    13441 agagggcctt ccatgtacga cgccgccgac gccgacgttc cgcacccggt ccggatcgcc
    13501 gaccgcgccc cgcgccgccg cggcccgcgc acggtggcgg tcgcggcggc ggtgggcgcg
    13561 ctgctggccc tcgccgggtg cggtgggggc gggggcgggg gcggtaaggc ggacgccaag
    13621 gcggagaccg ggggcgacgc cacccggccc agcgcgagcc ccgcgcccaa ggccgcgcga
    13681 gtggaacagc tcgcctcggc cgccggctgc aagccggagt tcaccaccaa ggtggacgac
    13741 taccgccagg ccgtctgcaa gaactccaag ggcaagttcg tcttcctcga cttcgtcacc
    13801 gccaaggggc agcgcgactg gctggagacg gcgcagatgt acggcggggt gtatctggtg
    13861 ggcaaccgct gggtgctgtc gtcctcgccc cggaagaaca tggagcggct ccgggacgag
    13921 ttcggcggca ccatcgaagg aggcacctcc tacggaagcg cctccggcac cccccagtaa
    13981 tcacccgccc atcgctcgac gcagacccct taggcgcagc tacgtctacg ggatgtccgg
    14041 cagaccacgc gatggagccg cctatcggcg ctttcccctc cccgcccctt cccgatacca
    14101 ggggctccgc ccctggaccc cgagacctgg ggcgcagccc cgacccgggg tctgggtctg
    14161 gggtctgggg tacagccccg cccgggggcc ggggtctggg gcagagcccc ggcccgccgc
    14221 aggcgtgtgc gtagcgccgg gcgcccccta tacgtcggcc agcgcgcgta gcgtcgccgc
    14281 ctcccggggc agcagccgtt cgtcgtcgcc cggcacgaac tccagcagga catccagcgg
    14341 tggaccgccg ccggaccccg tggcgcggtg tgcgcgcagt cgctcgaagg cgccgcgcca
    14401 cagcggggcg cgggcggtca gcggcagccg ggtggtcccg ggccaccagg agaaggcgtg
    14461 caccgcggcg atccggtcgc ccagcgcttc cagccccttc agggcctcgg cgtcgggcat
    14521 gtcgacgggc ggctgccagt aggtggcgac ctctgcgcgg tccacctccg ccagcagccg
    14581 gagggtgtgg tccgcgccgt ccgtcagggt gttgcggtgg aactcatagg cgagttccac
    14641 tcccgcgtcc gcggcgagcg cggctgcgcg ccgggtgtcc tcgacaaccg cgcggcgccc
    14701 ggcgggcgag gtctcccgag tgccggtggc gcccgcccag atccggacgc gcggtgcgcc
    14761 gagcgccacg gcggagcgga gcaccgcgtc gaactcctcc ggatcgccgt gcccggcgcg
    14821 gtagtaggag ccgtacgagg cgacggcgag cccggcgtcc acggtggcgt cccggacccg
    14881 ccgcgcggtg tcctcgtcgc cgggcggcac atggacatcg ccgccccatt cgacgcactc
    14941 cagccccgct ccggccaccg ccgcgatcac ccggtccgga tcgagtcggc gcagggtcac
    15001 cgagcagatg ccgagccggg tcatgcgctc acctcggtga cggtgggcgc gtcgcccgtg
    15061 ccgaagtcca ccacgacgtc cgcgccgtcc ggcagccgca cggtgaccgc gagcccgtgc
    15121 acctcggcgg tggcgaggtc ggcgaggggg gccgggtcgc gttccccggt gagcgaggcc
    15181 agggcgacga agagggtgcc ggaggggttc tcgccgacgg tgccggtgag ttcggggacg
    15241 agggcccacg gcccgtacgc ggtgccctgc ggcgcccgga ccgcggtggc ggtctcccag
    15301 ccgtgcagtc cgaggagttg accggtcacc gctcccccgt ccagccgcac ctcggcgccc
    15361 cgctcggcgc cccgctcggt ctccgccggg gtgcccgggg gcagggcgac ggcccagccg
    15421 ctgtggtgga tccgggtccc ggcggggacg cccacggtgc ggtggacgcg cacttcgagc
    15481 cggccgcgga cgagggtcag gctgtcgatg cggagcgcgg gcaccttggg gccgccgtcg
    15541 gggaaggcgg gggtgtggga ggaggcgagc cagtcggggc ccgcggccag cgggtggatg
    15601 cgcagccggt cgctgcgcac tccgcgcact tcgagggcga tgtggttgtc gggggcgttg
    15661 tccgcgctgg tgggcccggt acgggtggag taggcgagcc gggcgtagag cgggtcggcg
    15721 ggggcgtact cggcctccca ggggcggagc ttgtagctgc cgtggttgtg cagccgcacc
    15781 agcccgtccc ggcccgtggt ctggatgagg agcccggtgg cgggcagcgc cagcgcccgg
    15841 tcgggaccct cggcgggcgc ggcctcctcg ggctcggtcc acaccgggtg gcctggcggg
    15901 agcagcaggg cgaggaaccc cttggaggcc cagtagggcg agccgggccc ggagtagcgc
    15961 tggagggtcg cggcgtgcgg cccgtaccag ccaaggctca gcaccccgtc ctcggtcagc
    16021 gccccgcggt ccaggaagtg gcgcagcgcc ccgctgagga cgcggcgggt ggccccgggg
    16081 gcgagggggg tgtggccggt gagcgcgccg agggccaccg aggcgccggc ggcgaagcgg
    16141 taggtgaggg agcggccttg gtggatgggg gcgccgtcgg cgtcgaagag cagcgagaag
    16201 ctctccagga aggtgcgcag ccgggggccg agccggtcca ggagcgcgcc gtcggcggcg
    16261 agatgggcgt gcagcagcgg gtacatgtgc agcgcccagc cgttgtagtg gtcgaacgac
    16321 cgcccgtcgc cgtcggagta ccagccctgg ccctggtacc agccctccag gagggcgagt
    16381 ccgcgctcga tggcgcgggc cgtctcggcg tcgccgcgcc cggcggactc caggaatccg
    16441 gccacggtga gggggaagag gtaccagttg ttgggggcgg gctggtggcg cagcgcgccg
    16501 cgcagccact cctcggtgcg gtcctgggcg tcgggcggca gggcgtccca ggtccacggc
    16561 gcggtcagcc gcaggcccag ggccaccgac gcggactcca ccatgggctg gccgtgggcg
    16621 ccgtgatggc cgatcaccgg ccaggactcg gcgtcgccgc gccctggggt gagggtgccg
    16681 cgggcgatcc cggcggtgta gcgctccagg atgccgtgct ggtcctggcc cgaggccccg
    16741 gcggcgcgga aggcggcgag gaggaaggtg cgggcgaacc cttcgagccc gtcggagcgc
    16801 accccggact tggacggggg gccgggcagg tcgatcaggg cctggccggg ggtggcgtaa
    16861 cggagggcgg cgtgcagcat gccgtccgcg gcggcctccc agtgggcacg ggtgtagccg
    16921 gtgtgggggc tgagggcgcg gtcgtcggcg gggaggagga aaggcagctc gggcatggcg
    16981 gggtgggcct cctggggtcg cgggacgtga gagggcgcgc ggatgggtgg agcggggcag
    17041 gagcggggcg ggggacgagc ggggcgaggc ggaggaggga ggggatgcgg aagggcatat
    17101 cccggcggtg gccgggagga atcggcgccc gacggcaacg ccgccgggcg ccgcccatcg
    17161 ccccgcgacg ccgccccgcc tgtccgcacg cttatgccga gcggtcgagg atctccggtc
    17221 gtacggggtg ggcgaaggcg cggccggtga cgtagttctc gatctcgccg atggcgaggt
    17281 cggccaggcg gcgcagctca ccgctcttgg atccggcgat atgcggggtg atcagcgcgt
    17341 tgtcgcagtc gtacagcggg gagtcggcgg gcagcacctc cggcacggtg acatcgagga
    17401 cggcgcggat ccgcccggcc accacctcct cggtcagcgc cgcctggtcc accaccgccc
    17461 cgcgcgcggt gttgatgagc gtggcgccgt ccggcatggc ggccagcagc tcccggccga
    17521 ccagcccctc ggtggcgggc agcagcgggg tgtggacgct gatgacatcg ctgcgggcga
    17581 agagttcccg caggggcacc cgccgcgtcc cgagctcctc cgcctcccgg gccgtgacat
    17641 acgggtcgta cagcaggacg tcgaggtcgt acgggcgcag cagctcgatc acccggcggc
    17701 cgatggcgga ggcgctgagg atgcccaccg tccggcggta gttgccggcg tcggggtagc
    17761 gctcgttcca gtcgatcgcg cgccgctcgg cgcggtagtc ccgggcgatg tccaggacgc
    17821 gcttgttggc cagcaggatc atggcgacgg tgtactcggc cacgggcagg gcgttggcgg
    17881 cggccgccga ggagaccgag atgccgcgct cccagcaggc gtcggtgatg tggtggcgta
    17941 cggtgcccgc ggtgtggacc acggcgcgca gccggggcgc ggcgtcgagc gcgcggcggt
    18001 ccaggggcgg gcagccccag ccggtgacga gcagttcggc ctcggcgagg gcccggtgcg
    18061 cggccggggt ggtgaagtcg tccaggacga gggcggggtc gaggtcgagg agttcggtga
    18121 gccggtccgg gctcacggac ccgagcacct gccgggcgga ccgttcggcc atggccagga
    18181 cggcacgggg gcgggtgcgg gtgcgggtgc gggcggagat ttgcggtgcg gtcacttgac
    18241 gctccctgcg gtcagtccgg acttccagta gcgctgcaac aggacgaacg ccacgatcag
    18301 cggcacgacg gcgagcagtg agccgatgac cacgaggggg tagtactcgg gcgagacggt
    18361 ggcggcgctg ttccaggtgt agaggccgag gctgagcgga tagaggtgct ggtcggagag
    18421 catcaccatg ggaaggaaga agttgttcca gatggccgtg agctggaaga ggaagacggt
    18481 cacgaagccg ggcccgagca tccgcaggct gacccgtaca taggtctgca actcgcccgc
    18541 gccgtcgacc cgggacgcct ccagcacctc gttgggcaca tagccgctgc tgagcaggcg
    18601 ggcgaggtag acgccgaagg ggttgaagag caccgggacg aagaccgacc agaaggtgtt
    18661 gaccatcccg atctcggagg ccaccagata gagcggaagg gccagtacgg tcggcggcac
    18721 catgacgccg gtgaggacga gcccgaagaa cttctccttg tgccggaact cgtacttgtc
    18781 gaaggcgtag cccgcggcca cgctgatcag cgagccggtc agggcgccga cgaccgcgta
    18841 cagcaggctg ttgacgtacc actcgccgta gagcccgccg tccatggaga acagatcgga
    18901 gaggttgcgg gcgaaggaga agtcgccgag ggagaagatg tcgctgccga agagggcgtc
    18961 ggcgttcttg gtggacgcca gcagcagcca cagcgcgggc agcatggtgt agagggcggc
    19021 gatcaggacc acggcgttgg cgacgccgcg gctggtcagc ctggagccgc cgaagggctt
    19081 gccgagcggt ctggacgacg ggccccggga ggcgcccacg gggctggtgg gtgtggtcgt
    19141 ggtcatcacg ctctccgttc ccggcggccc gaccagcgcg tcacggcgta cgacagcacg
    19201 caggtgacga tcaggaggat gacggaggcc gcggaggcga gtccgtagtt gttgcggcgg
    19261 aaggccgcgt cgtagatgta catgttcggg gtgaagcggg agttgaccat cggggtggcc
    19321 tggttcatca gcatgggctc ggtgaacagc tggagcgcgc cgatcaggga gaacatgcag
    19381 accatcacca cggagccccg gatgatgggc accttgacct ggagcgcggt gcggatgccg
    19441 ccggcgccgt cgatggtcgc cgcctcgatc acctcgcgcg gcagcgcctg gagcgcggcg
    19501 tagaagatga ccatgttgta gccgaggccg ctccacaggg cgatgttgac gatcgacggg
    19561 agcacggtgt gcaggcccag gaagtcgacg gtgatgtcgg cccgggcgaa gagtttgatg
    19621 accgggctga gccccggggt gtagaggtag agccagatga cggccgcgat gatgccgggc
    19681 acggcgtgcg gcagatagag cagcatctgc gcggtgcgcc gcagccggac caggccggag
    19741 tcgagcagca ggccgagcgc gagcgcgctg atgaccacga gcgggacgaa gatcaggcag
    19801 tagagcgcga tggtgccgaa cccggagagg aagctggggt cctcgagcac cgagagatag
    19861 ctgcgcagtc cggtgaagac cgtacgcccg ccgccgaagc cgagtccctc atggtcctcg
    19921 gagaagaagc tgaggtagac ggcgtagccg acggggatca ggaacacggc gacgagcagt
    19981 gccgtgaagg gggtcagcag aacggcggcg gcgatccgct gctggcgtcg tccggttctg
    20041 cgggtcgggt cggcggcggc cgccgatggc ggcgccgtcc ctttcgagga cgtgacggtg
    20101 ctcacgggtt acctcggctc aggcgggatg aggtgcggct cggggtgggg cggagatatg
    20161 ggaggcgtgg cggctcagcg ggtggtgacc gacaggccga ggctcttcag atcgggcatg
    20221 gttctgctct gggcctcgcg cagggcctcc aggatggtgc cgctgccggc tccggcgcgg
    20281 gcgagcccgt cgtcgccgga ggtggtggtc gagaccatcc gcggccccca cttccaggtg
    20341 ggggagatca tctcggcctg ttcctggaag aggccgaaga tgtcctggcc ggagtagtag
    20401 ctggtgtcca tctccttacg ggcgaccggg accagaccgg gcacggacgg gaaggcgctg
    20461 ctgacaccgc tggccagttt ggccttcagg gcgccggggc tggtcaccat ccaggagatg
    20521 aactccatgg cctcctcggg atggtgactg ttcttggtga ccaggaaggt cgagccgccc
    20581 tgtgtgctca ccctgggttc ggcggggtcc cattgcggca tcggcgcgat ggcccatttg
    20641 cccttgccgc cgggggtgga cgacatcatc gagcccgcgg cccaggcgcc ggccagatag
    20701 ccggccgtct caccgctctg catatgggcg cgccactgct ggctggagcc gggttccac
//
