LOCUS       NZ_CP025018            10996 bp    DNA     linear   CON 12-FEB-2024
DEFINITION  Streptomyces sp. M56 chromosome.
ACCESSION   NZ_CP025018
VERSION     NZ_CP025018.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. M56
  ORGANISM  Streptomyces sp. M56
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 11742376)
  AUTHORS   Kim,K.K.Hyun., Klassen,J.L., Ruzzini,A.A.C., Beemelmanns,C.C. and
            Clardy,J.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-NOV-2017) Leibniz Institute for Natural Product
            Research and Infection Biology - Hans Knoll Institute,
            Adolf-Reichwein-Strasse 23, Jena 07745, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-AUG-2014
            Assembly Method                   :: HGAP v. AUG-2014
            Assembly Name                     :: M56v2
            Expected Final Version            :: yes
            Genome Coverage                   :: 94.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/12/2024 06:37:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,558
            CDSs (total)                      :: 9,473
            Genes (coding)                    :: 9,167
            CDSs (with protein)               :: 9,167
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 86 of 306
            Pseudo Genes (incomplete)         :: 261 of 306
            Pseudo Genes (internal stop)      :: 28 of 306
            Pseudo Genes (multiple problems)  :: 61 of 306
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:58:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1264709
            Orig. end                         :: 1275705
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP025018.1.
            Annotated using prokka 1.11 from http://www.vicbioinformatics.com.
            Bacteria and source DNA available from Christine Beemelmanns.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10996
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="8"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10996
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10996
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5996](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5996)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    535..550
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.62"
                     /tool="antismash"
     misc_feature    complement(536..549)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.58"
                     /tool="antismash"
     gene            539..1732
                     /locus_tag="CFP59_RS05320"
                     /old_locus_tag="CFP59_01046"
     CDS             539..1732
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018956672.1"
                     /locus_tag="CFP59_RS05320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_01046"
                     /product="histidine kinase"
                     /protein_id="WP_231981139.1"
                     /transl_table=11
                     /translation="MPVRMESSTVVRRLRRVVRWWRSRGLVTRDRELALALVLTALVFV
                     PALSRISSTFGDLPARHGDAFAVLLSLGQTVPLVVRTRRPAVCLAVVGPSFAVHESLGY
                     PPTFGSMGLYLALYGAGSHLSRHRNVVAALASAGYLAFAITLHTLDSPGRFLDYLIFFL
                     ALAAFWLLGSYVRARRADEAERRRLAALAATAAERSRIARELHDVVTHHVTAMVVQADA
                     AQFLTESPGRVTEGLTAISGTGRQALGELRHLLGVLEATGESAPTRRTPALGTIEELVA
                     RTRLGGQPVELVEEGERPPMAAGAELAAYRVVQESLTNAVKHAAGHATEVWVRYHRDGV
                     DIDVTTDGPSRVAHAPDGRGLNGLRERVGLVGGELEAGARPGGGFSVSARIPAGSGS"
     gene            complement(1862..2725)
                     /locus_tag="CFP59_RS05325"
                     /old_locus_tag="CFP59_01047"
     CDS             complement(1862..2725)
                     /EC_number="2.1.1.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003983473.1"
                     /locus_tag="CFP59_RS05325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_01047"
                     /product="SAM-dependent methyltransferase"
                     /protein_id="WP_100805103.1"
                     /transl_table=11
                     /translation="MTDGHERLAGVSSTALFVGITRAAESRRQDRLFEDPLATRFVDAA
                     GAEQQAAWTQGEGRLFTEAMGDYFALRTRYFDDYFMQACAAGCRQAVLLAAGLDTRAFR
                     LHWPAGTRLFELDQPDVLDFKEHVLQGEVPRCEREVIAADLREDWPALLMKHGFRADEP
                     TAWLVEGILVYLTARDADHLLDRIGALSAPGSHLAVEHVTRSMVNTDQAQEAAAASPGG
                     IMNMLSGLWKNEMAQPPAEWLADHGWSAEEELLTDLAKRHERPVPPAFDPALPGTGRVR
                     LLAADR"
     gene            complement(2834..4090)
                     /locus_tag="CFP59_RS05330"
                     /old_locus_tag="CFP59_01048"
     CDS             complement(2834..4090)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608207.1"
                     /locus_tag="CFP59_RS05330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_01048"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_100805104.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 2.4e-17, bitscore:
                     62.3, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRPVIIDKVRIFDGRAMLGPGSVEFADGVITRVTTEPAPVAPASA
                     DVIDGTGMTLLPGLIDAHTHVFGAESNLELALAFGVTTELDMFMGPPEATQALCAAAGE
                     RADLADMRSSGLVASAPGGHPGVTMPLLPTVGGPERADAFVAARQAEGAHFIKIIVDDG
                     ANHGMSLPALDRATVTALAEAARRRGLLTVAHVSALWSARLALDSGVDMLTHLPLEAAL
                     DEAFVSRTADERRVVIPTLAMLEASAPETSASARAGRSLADDPRIAGYLPDHARTAMAE
                     GHEGLCVEHASADHDFGHALSSVAALRGAGVPVLAGTDTNYRPDRACPVVHGASLHTEL
                     ALLVRAGLTPAEALTAATSAPAEHFGLFDRGMIAPGRRADLVLVEGDPTKDIGRTRSIE
                     AIWRGGVRFDREAYRKRTA"
     gene            complement(4096..5013)
                     /locus_tag="CFP59_RS05335"
                     /old_locus_tag="CFP59_01049"
     CDS             complement(4096..5013)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /locus_tag="CFP59_RS05335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_01049"
                     /product="UbiA family prenyltransferase"
                     /protein_id="WP_100805105.1"
                     /transl_table=11
                     /translation="MAELSDVLTASRVPARLGRFVLRMFRPQIYVTYGVLWTLALEGLA
                     EALSGSAAHWTPSWSTVVRAVSVVLALLFARMVDEQKDLEYDRTHNPDRPLVTGAITAA
                     ELRGAMAFSTVVVVVLNTFVSAFSVLLILLALGYTLFLVALERRFPRLGERLIANLLVS
                     YPVQLLLSGYLYVSLLTGDEIGGDGRAAPLMAMFACAFLQFEFARKTEWRRDPRARLYS
                     EVLGPRGSAAVSLALALAAVALGLLLFGARGWPPALSIVFPALGAWRFLVRRKPSGLML
                     PAMGFVLFFNLLCFYGSTAIAIGR"
     gene            complement(5001..5996)
                     /locus_tag="CFP59_RS05340"
                     /old_locus_tag="CFP59_01050"
     CDS             complement(5001..5996)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608204.1"
                     /locus_tag="CFP59_RS05340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_01050"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_100805106.1"
                     /sec_met_domain="AfsA (E-value: 1.7e-18, bitscore: 65.6,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDTVEFSQTVPRELVHRLSLAEVFLTGVLARDEDTLDIGVQIPRS
                     HVFYRDTAGCRHIYDPMVLVEAVRQSLLMLGHDLFERPFGTKFILRDIALYDADITALA
                     VRDVPTDVVLRCCIVRRFRGREGLKGARLSYSALIGDRVAATLEGSMSWLTPAEWRAAR
                     EEGRATLELPVQATFTACEPGPRIGEALVDRRDTSNVVISPPECHLMPDGGETRTARLL
                     VDTSHPVLFDHPLDHVPGMLTLEAFRQLSIATAVQTGALPSASAVLTGLKARFTGFGEL
                     DLPITCESVQAEGDGGSAVLRCAMKQAGRTIADGELRLTRAPAEAGRWRS"
     gene            complement(6012..7016)
                     /locus_tag="CFP59_RS05345"
                     /old_locus_tag="CFP59_01051"
     CDS             complement(6012..7016)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984548.1"
                     /locus_tag="CFP59_RS05345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_01051"
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="WP_100805107.1"
                     /transl_table=11
                     /translation="MTEALTDDRPFGDAISPALSRDPRKRTLFGSDTYFLESVEQLGTL
                     TADVDGFLHRHAALLLKPDAVVSRQLPTTVDWLARNGFRIVAAERTRLTRTVVRSLWYF
                     QWNLATPERRRLADLFTDSCDSVVLIVRQDADGAEAPPSAPASVVLTERKGPTDPRARV
                     PGQLRHETGRYSYLLNLVHTPDEPADVARELGIYFDSGLRRRVYTSALVGEDLSVRARE
                     LGERLHAEVPERDLTFEPAAERLERAVAEAEESLAPGAVRDELRQARLAARDREGHRRL
                     LEAVWRARLPLDAWDMVIVGSHVLPMKRTGFAPVLGGVSVADWHQHQARPAVR"
     gene            complement(7013..8185)
                     /locus_tag="CFP59_RS05350"
                     /old_locus_tag="CFP59_01052"
     CDS             complement(7013..8185)
                     /NRPS_PKS="Domain: NAD_binding_4 (8-232). E-value: 3.3e-50.
                     Score: 162.5. Matches aSDomain:
                     nrpspksdomains_CFP59_RS05350_NAD_binding_4.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NAD_binding_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608202.1"
                     /locus_tag="CFP59_RS05350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_01052"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_100805108.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 6.4e-17, bitscore:
                     60.7, seeds: 37, tool: rule-based-clusters)"
                     /sec_met_domain="NAD_binding_4 (E-value: 5.2e-48, bitscore:
                     162.5, seeds: 102, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRRRPRVLVTGAAGLVGAEVTARLAAAGHQVTALVHNNPVLVRND
                     GTALEAGAVTCLPGDVTEPRLGLPEAEYRALAAGGLDRIVHCAAVTDFGLPDEVYQAVN
                     VDGTAHVLELARAGGVPLVHVGTAYVCGERDGLIRETELDEGQRLANAYEDSKFRAETL
                     VHKAQADGVRAAIVRPSIVVGDERTGVVRDYKNIYVVLKLATEGKVRSIPGQYEARLDL
                     VPVDYVADIITEVTQRFEEAAGRTFHAVGAPLTLRDFSDVMAEYPPFHVPRFIPPTSFD
                     TQRLPRRERVYYERVMTLYESYFRRRMRFDDSHTAGLVTRRPPGDGQDFLRTLIDNCLE
                     TGYLGVPMADATEVLARLNITGPNATAAAHATGAPHGTGSTVSDANGVGR"
     aSDomain        complement(7490..8161)
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CFP59_RS05350_NAD_binding_4.1"
                     /evalue="3.30E-50"
                     /label="CFP59_RS05350_NAD_binding_4.1"
                     /locus_tag="CFP59_RS05350"
                     /protein_end="232"
                     /protein_start="8"
                     /score="162.5"
                     /tool="antismash"
                     /translation="VTGAAGLVGAEVTARLAAAGHQVTALVHNNPVLVRNDGTALEAGA
                     VTCLPGDVTEPRLGLPEAEYRALAAGGLDRIVHCAAVTDFGLPDEVYQAVNVDGTAHVL
                     ELARAGGVPLVHVGTAYVCGERDGLIRETELDEGQRLANAYEDSKFRAETLVHKAQADG
                     VRAAIVRPSIVVGDERTGVVRDYKNIYVVLKLATEGKVRSIPGQYEARLDLVPVDYVAD
                     II"
     gene            complement(8182..8469)
                     /locus_tag="CFP59_RS05355"
                     /old_locus_tag="CFP59_01053"
     CDS             complement(8182..8469)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CFP59_RS05355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="CFP59_01053"
                     /product="acyl carrier protein"
                     /protein_id="WP_141729439.1"
                     /sec_met_domain="PP-binding (E-value: 2.7e-05, bitscore:
                     23.7, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSSTGTHLLNDDATRNTVYAIVGAMAPAGGVHLADDTELITDLA
                     YDSLRLIELTLALEDGFGLAELGQEKTATVTTVGDIVRLVTEARHEEEGR"
     gene            complement(8486..10141)
                     /locus_tag="CFP59_RS05360"
                     /old_locus_tag="CFP59_01054"
     CDS             complement(8486..10141)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608201.1"
                     /locus_tag="CFP59_RS05360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CFP59_01054"
                     /product="hypothetical protein"
                     /protein_id="WP_100805109.1"
                     /transl_table=11
                     /translation="MILSEDHLQNLLDQTIPRIREAANCAVVLEGSIAEGFGNTSSDID
                     FLLIADSDADLPTMPSLLFLDGRRVEVRTRSVRQLTEQFSSVTSATHGHPGALSEDLLN
                     RCQRLLRSFPLRNPDLVAKVKGLMSFEDFQDTMREWWAHHARQSIRHALALRELGQDEE
                     AAAWTEAGLLQAVKSWAAGRGETYLEPKWLPMQLDRMGDQPLCARYRELASLDASGLGP
                     AEYIAAGARLTAELGVTGAEPDPERVTVARAGGVTTWPTGDRVHVVRDKQDVFVLGDGA
                     ARAWRSVVFGRPLRSVLAAAEASGAPQAGPLIAQFLRFGLAKAAWKGEGPIVPAMPLAA
                     PSGAVTPPPSTARPIVTVGGAAAGGERAIDLVPMPARRFSAAAMTLVWSNVLVENARED
                     LIGALDREQWSVAELSARRMLRAALRGVLSAYGVNPLPPDSEVVRRLSLLPAGAATDEI
                     RPKARYLETLAIASAAQGGAALTALDDFVALVRHTTGAHGFPSSFDSSDGWRQTLEIGY
                     DWLRLGAHLDADLPIDEASDLLASGGAQPHLATT"
ORIGIN
        1 aaccgctgca acaccttcat ggtcgtcagg tattcctcgt cggagatgcc cgcgcggcgt
       61 tccgcctcca gttcgccctg gagggccgcg gccttgtcgt agaacgccct gccctccgcc
      121 gtgatgccca gacgtccctc ggcgtcctcg gtgatccagc cccgggcgat ggtcgcgtcg
      181 atctccgact ccatgacgtc cgcacccgag tccagatagt tgcggaggag gcgggacacc
      241 tcaccgcggg ttttggcggt gtccgcgttc gcgatctgcg ccaggaccca ccactgcggc
      301 tgggtggtgc cgatctcggc gagagtggac tggatgcgag tgacaacggc cttgtaggcc
      361 gcccaactcc agtagccgat gggctgccgt agcagttcgg cgtcgctgtg ggagtactcc
      421 atgaccggtc cctccgttat tgcctgaact cgtttgaacg cgtgtgaact cgctcgacgt
      481 cgagatcgac cgtaaaagtt caacccgact tcaagtcaag ccccattagg ctcctcgcat
      541 gcccgtgcgt atggagtctt cgacggtggt ccggcgcctt cggcgggtgg tgcgctggtg
      601 gcgctcgcgg ggcctggtga cccgggaccg tgagctcgcg ctcgcgctcg tcctcacggc
      661 gctggtgttc gtcccggccc tgtcgcgtat ctcctcgacg ttcggcgatc tgcccgctcg
      721 gcacggcgat gccttcgcgg tgctcctgtc cctggggcag accgtgccgc tggtggtgcg
      781 cacccgccgg ccggccgtat gcctggcggt ggtggggccg tcgttcgcgg tccatgagtc
      841 gctcggctat ccgcccacct tcggcagcat ggggctctac ctcgcgctgt acggcgccgg
      901 atcgcatctg tcccgccacc ggaacgtggt ggcggccctg gcgagcgcgg gctacctggc
      961 gttcgcgatc accctgcaca cgctggactc ccccggacgg ttcctcgact acctcatctt
     1021 cttcctcgcg ctggccgcgt tctggctcct cggcagctac gtccgtgccc ggcgtgcgga
     1081 cgaggccgag cggcggcgtc tcgccgcgct cgcggccacc gcggcggaac ggtcccggat
     1141 cgcacgcgag ctgcacgacg tggtgaccca ccatgtgacc gccatggtgg tgcaggccga
     1201 cgccgcgcag ttcctcaccg aatcgcccgg ccgtgtcacc gagggactga ccgcgatcag
     1261 cggcaccggc cgccaggctc tgggcgaact gcggcatctg ctgggcgtgt tggaggcgac
     1321 gggcgaatcg gcgcccacgc ggcggacacc cgcgctcggc accatcgagg agctcgtcgc
     1381 gcggacccgg ctcggcggtc agccggtcga actcgtcgag gagggcgaac gaccgcccat
     1441 ggcggccgga gccgaactgg ccgcctaccg ggtcgtacag gagtcgctca cgaacgccgt
     1501 caagcacgcc gccggacacg cgaccgaggt atgggtccgc taccaccgcg acggggtgga
     1561 catcgacgtg accacggacg ggccgtcccg tgtcgcccac gctccggacg ggcgcgggct
     1621 caacggactt cgagaacggg tgggcctggt cggtggcgag ctggaggcgg gcgcgcggcc
     1681 cggcggcggg ttcagcgtca gcgcccggat tcccgccggg agcggctcat gatccgggtg
     1741 ctgatccgtg cgggccggtg cgccccgcca gccgggatgc cgggatcgga cggtgatgtc
     1801 gctgtggatc ggcgtcccgg ggaatgtcgc tgcccttcgg cgctccgggg aagacgagcg
     1861 gtcagcggtc ggccgccaga agacgcacac ggcccgtgcc cgggagggcc gggtcgaagg
     1921 ccggggggac ggggcgctca tggcgcttcg ccaggtcggt gaggagttct tcctcagcgc
     1981 tccagccgtg gtccgcgagc cactcggcgg gcggctgcgc catctcgttc ttccacaggc
     2041 cggacagcat gttcatgatg cctcccggtg aggccgccgc tgcctcctgg gcctggtcgg
     2101 tgttcaccat cgaccgggtg acatgctcca ccgcgaggtg gctgcccggc gcggacagcg
     2161 cgccgatccg gtcgagcagg tgatccgcgt cgcgcgcggt gaggtagacg agtatgccct
     2221 cgaccagcca ggccgtcggc tcgtccgcgc ggaagccgtg cttcatcagc agcgcgggcc
     2281 agtcctcgcg cagatccgcg gcgatcacct cgcgctcaca gcgcggcact tcgccctgga
     2341 gtacgtgttc cttgaagtcg agcacatcgg gctggtccag ttcgaagagg cgtgtcccgg
     2401 ccggccagtg caatcggaag gcacgggtgt ccagaccggc cgcgagcagg accgcctggc
     2461 ggcagcccgc cgcgcacgcc tgcatgaagt agtcgtcgaa gtaccgggtg cgcagggcga
     2521 agtagtcgcc catggcctcg gtgaacagcc tcccctcccc ctgggtccag gccgcctgct
     2581 gctcggcgcc cgcggcgtcg acgaaacgcg tggccagggg gtcctcgaac agccggtcct
     2641 ggcgccggct ctcggccgcg cgggttatgc ccacgaacag cgcggtgctg ctgacccccg
     2701 ccaggcgctc gtgtccgtcg gtcacgtggt tctccccttc gtatggtttc ctgtcatacc
     2761 gcttccgctc atgggcgggt cgctcctgtc catgagcgtg tggtcctgtt gatgggcgtg
     2821 tcgctccccc tcatcaggca gtgcgtttgc ggtacgcctc gcggtcgaag cgcacgccgc
     2881 cgcgccagat cgcctcgatc gaccgggtgc ggccgatgtc cttcgtgggg tcgccctcga
     2941 cgaggacgag gtccgcgcgc cgccccggcg cgatcattcc gcggtcaaag aggccgaagt
     3001 gttcggcggg ggccgaggtc gccgccgtga gcgcctccgc cggagtcagt ccggcccgga
     3061 cgaggagggc gagctcggtg tgcagactgg cgccgtggac gaccggacag gcgcggtccg
     3121 gccggtagtt ggtgtcggtt cccgccagta cgggcacacc tgccccgcgc agagcggcca
     3181 cgctcgacag ggcgtggccg aagtcgtggt cggcggaggc gtgctccacg cacaggccct
     3241 catggccttc cgccatcgcc gtacgggcgt gatccgggag gtagccggcg atccgcggat
     3301 cgtccgccag tgaccgtcct gcgcgcgccg aggcggaggt ctcgggtgcg gacgcctcga
     3361 gcatggccag ggtggggatg acgacccgcc gctcgtccgc ggtccggctg acgaacgcct
     3421 cgtccagagc cgcttccagc ggaaggtgtg tcagcatgtc cacgccgctg tccagggcga
     3481 gccgtgcgga ccacagggcg gacacatggg ccacggtgag cagaccgcgg cgccgcgcgg
     3541 cctcggcgag cgccgtcacg gtcgcccggt ccagagcggg cagcgacatc ccgtggttcg
     3601 cgccgtcatc cacgatgatc ttgatgaagt gtgcgccctc cgcctgtctc gcggccacga
     3661 acgcatcggc ccgctcgggc ccgcccaccg tcggcagcag cggcatggtg acgccggggt
     3721 gcccaccggg cgcgctcgcc accagccccg agctgcgcat atcggcgagg tcggcacgct
     3781 cgccggccgc cgcgcacagt gcctgggtgg cctccggcgg gcccatgaac atgtccagtt
     3841 cggttgtcac accgaaggcc agcgccagtt cgagattgct ctcggcgccg aacacatgcg
     3901 tatgcgcgtc gatcagcccc ggtagcagcg tcatccccgt tccgtcgatc acatcggccg
     3961 acgccggtgc caccggggca ggttcggtcg tgacacgtgt gatgaccccg tcggcgaact
     4021 ccaccgagcc ggggcccagc atcgctctcc cgtcgaagat ccgcaccttg tcgatgatca
     4081 ccggccgcat ccctttcacc gtccgatcgc gatcgctgtg gacccgtaga aacacaacag
     4141 gttgaaaaag agcacaaagc ccatggccgg gagcatcagc ccggatggtt ttcggcggac
     4201 caggaagcgc cacgccccca gcgcggggaa gacgatgctc aacgcgggcg gccacccccg
     4261 tgccccgaac agcagcagcc cgagggccac cgcggccagg gcaagggcga gggagacggc
     4321 agcgctgcca cgcgggccga gcacctccga gtacagccgt gcccgcgggt cccgccgcca
     4381 ctcggtcttc cgcgcgaact cgaactggag gaaggcacag gcgaacatgg ccatgagggg
     4441 ggcggcccgc ccgtcaccgc cgatctcgtc gccggtcagc agcgagacat agagatagcc
     4501 ggagagcagc agctgcaccg ggtagctgac gaggagattg gcgatgaggc gctcgccgag
     4561 ccgtgggaac cggcgctcca gcgccaccag gaacagcgtg tatccgaggg cgagcaggat
     4621 cagcagcacg gagaaggccg agacgaacgt gttgagcacc acgacgacca ccgtgctgaa
     4681 cgccatggcg ccgcgcagct cggccgccgt gatggcaccc gtcaccagcg gccggtccgg
     4741 gttgtgcgtg cggtcgtact ccagatcctt ctgctcatcg accatacggg cgaacagcag
     4801 ggccagcacc acgctcaccg cgcgcaccac ggtcgaccag gagggcgtcc agtgcgcggc
     4861 ggacccggag agcgcctcgg ccaagccctc cagggccagg gtccacagca caccgtaggt
     4921 gacatagatc tgcgggcgga acatccgcag aacgaagcgg cccaggcgtg ccggtacccg
     4981 ggaagcggtc aggacatcgc tcaactccgc caccgaccgg cttccgcggg ggcccgcgtg
     5041 aggcgcaatt cgccatcggc gatggtgcgt ccggcctgct tcatggcgca gcgcagaaca
     5101 gcgctcccgc cgtcgccttc ggcctgaacg ctctcgcagg tgatcggcag atccagctcg
     5161 ccgaagcccg tgaagcgcgc cttcagcccg gtgaggacgg ccgacgccga tggcagtgcc
     5221 ccggtctgca cggcggtggc gatgctcagc tgccggaacg cctcgagggt cagcatgccg
     5281 gggacatggt ccagggggtg gtcgaacagc acggggtggc tggtgtccac gaggagccgc
     5341 gcggtcctcg tctctccccc gtcgggcatg aggtggcact cgggcgggga gatgaccacg
     5401 ttcgacgtgt ccctgcggtc gaccagggcc tccccgatcc gcggccccgg ctcgcaggcc
     5461 gtgaacgtgg cctggacggg cagctccagc gtggcccgcc cttcctcgcg cgccgcccgc
     5521 cactcggcgg gcgtcagcca ggacatggag ccctccagcg tcgccgccac gcggtcgccg
     5581 atcagcgcgc tgtaggacag ccgtgcgccc ttcaggccct cgcggccacg gaagcggcgg
     5641 acgatacagc agcgcagcac cacatcggtg ggtacgtcgc gtaccgcgag cgcggtgatg
     5701 tccgcgtcgt acagggcgat gtcccgcagg atgaacttcg tgccgaacgg cctctcgaac
     5761 agatcgtggc ccagcatgag gagcgactgc cggaccgcct ccaccaggac catcgggtcg
     5821 tagatgtggc ggcagcccgc cgtgtcccgg tagaagacgt gcgagcgggg tatctgcacc
     5881 ccgatgtcga gggtgtcctc gtcccgggcg agcacgccgg tcaggaacac ctcggcgagg
     5941 ctcagccggt ggacgagctc ccgcggcacc gtctggctga attccaccgt gtccatgctt
     6001 ccccctccgt attaccgaac cgccggccgc gcctggtgct gatgccagtc ggccacgctg
     6061 accccgccga gtacgggagc gaatcccgtg cgcttcatcg gcaggacatg agacccgacg
     6121 atcaccatgt cccaggcgtc gagcggcagc cgggcccgcc agaccgcttc gagcagccgc
     6181 cggtggccct cccggtcccg ggcggcgagc cgggcctggc gcagttcgtc ccggaccgcg
     6241 ccgggggcca gggattcctc cgcctccgcc acggcccgct cgagccgttc cgcggccggt
     6301 tcgaaggtga gatcccgctc gggcacctcg gcgtgcaacc gctcgcccag ctcacgcgcc
     6361 cggaccgaca gatcctcgcc caccagtgcg gaggtgtaga cccgccgccg gagcccggag
     6421 tcgaagtaga tgcccaactc ccgtgccaca tcggctggtt cgtccggggt gtggacgagg
     6481 ttgagcagat agctgtagcg gccggtctcg tggcggagct ggccggggac gcgggcccgt
     6541 gggtcggtcg ggcccttccg ctcggtcagg acgaccgacg cgggtgcgct cggcggagcc
     6601 tcggccccat cggcgtcctg gcgcacgatg agcaccaccg agtcacagga gtcggtgaac
     6661 agatcggcga gacggcggcg ctccggagtg gccaggttcc actggaagta ccacagcgac
     6721 cgcacgacgg tacgggtgag ccgggtgcgt tcggcggcca cgatgcggaa gccgttcctg
     6781 gccagccagt cgaccgtcgt cggcaactgc ctgctgacca cggcatccgg tttgagcagc
     6841 agcgcggcgt gccggtggag aaagccgtcg acatcggcgg tgagggtgcc gagttgctcg
     6901 acgctctcca ggaagtaggt gtccgagccg aagagggtgc gctttcgcgg atcgcggctg
     6961 agggccgggg atatggcgtc gccgaagggc cggtcgtccg tcagggcctc ggtcatcggc
     7021 cgactccgtt cgcatcgctc acggtcgagc cggtcccgtg tggcgcgcca gtggcgtgtg
     7081 ccgcggcggt cgcgttcggc cccgtgatgt tcagccgggc gagcacctcc gtggcgtcgg
     7141 ccatcggtac gcccagatag cccgtctcca ggcagttgtc gatcagtgtg cgcaggaagt
     7201 cctgcccgtc cccgggtggt ctgcgggtga ccaggcccgc ggtgtgggag tcgtcgaaac
     7261 gcatccggcg tcggaagtag ctctcgtaga gcgtcatgac gcgctcgtag tacacgcgtt
     7321 cgcgccgggg cagccgctgg gtgtcgaagc tggtcggagg gatgaaccgc ggcacgtgga
     7381 acggggggta ctcggccatc acatcggaga agtcccgcag cgtcagcggt gcgcccaccg
     7441 catggaaggt gcggcccgcc gcctcctcga accgctgggt gacctcggtg atgatgtccg
     7501 ccacgtagtc gacgggcacc aggtcgagac gggcctcgta ctggccgggg atggagcgga
     7561 ccttgccctc ggtggccagc ttgaggacga cgtagatgtt cttgtagtcg cgcaccacgc
     7621 cggtgcgctc gtcgccgacg acgatactgg ggcggacgat cgcggctcgc accccgtcgg
     7681 cctgggcctt gtgcaccagg gtctcggccc ggaatttgct gtcctcgtag gcgttggcca
     7741 ggcgctggcc ctcgtccagc tcggtctcgc ggatcagccc gtcgcgttca ccgcacacat
     7801 aggccgttcc cacgtgtacg agggggacgc cgccggcgcg ggccagttcg aggacatggg
     7861 cggtgccgtc gacgttgacg gcctggtaga cctcgtcggg gaggccgaag tcggtgaccg
     7921 cggcgcagtg cacgatccgg tcgaggccac cggcggccag cgcccggtat tcggcctcgg
     7981 gcaggccgag ccggggctcg gtcacatcgc cgggcaggca ggtgacggca cccgcctcca
     8041 gcgcggtgcc gtcgttgcgc accagcaccg ggttgttgtg gaccagggcg gtgacctggt
     8101 gtccggccgc cgccagccgg gcggtgacct ccgcgccgac cagcccggcc gcaccggtca
     8161 ccagaaccct cggacgccgc ctcaccggcc ctcctcctcg tgccgtgcct cggtcaccag
     8221 gcgcacgatg tcgccgaccg tggtgaccgt ggcggtcttc tcctggccga gttcggcaag
     8281 tccgaagccg tcctccagtg ccagcgtcag ctcgatgagc cggagcgagt cataggccag
     8341 atccgtgatc agctcggtgt cgtcggccag atggacgccc cccgcgggcg ccatcgcgcc
     8401 gacgatcgcg tacaccgtgt tccgcgtcgc gtcgtcgttc agcaagtgtg tgccggtgct
     8461 tgacgtcaac gttcctccct cagcgtcagg tggtggcgag atgcggctgt gcgcccccgc
     8521 tggccagcag atcgctcgcc tcgtcaatgg gcaggtccgc gtcgaggtgt gcgcccaggc
     8581 gcagccagtc gtagccgatc tccaaggtct gccgccatcc gtccgaggag tcgaaggagg
     8641 acgggaagcc gtgcgccccg gtcgtgtgcc ggaccagtgc gacgaagtcg tccagcgccg
     8701 tgagcgccgc gccgccctgg gccgcggacg ctatcgcgag cgtttcgagg taccgggcct
     8761 tcggccggat ctcatcggtg gccgcccccg ccggaaggag ggagagcctg cggacgacct
     8821 cggagtccgg tggcagcggg ttgacgccgt acgcgctgag cactccgcgc agcgcggccc
     8881 gcagcatcct ccgggcgctg agttcggcga cgctccactg ctcacggtcc agggcgccga
     8941 tgaggtcctc gcgggcgttc tccaccagca cgttggacca caccagggtc atggccgcgg
     9001 cgctgaaccg gcgtgcgggc atgggtacga gatcgatggc ccgctcaccg ccggccgccg
     9061 ccccgcccac ggtgacgatg ggtcgcgcgg tgctcggcgg cggtgtgacg gcgccggaag
     9121 gggccgcgag cggcatggcg gggacgatcg ggccttcgcc cttccaggcg gccttcgcca
     9181 ggccgaaccg gaggaactgg gcgatcagcg ggcccgcttg cggtgcccct gacgcctccg
     9241 ccgcggccag cacactgcgc agcggccggc cgaagacgac ggaacgccag gcccgcgccg
     9301 ccccgtcgcc aaggacgaac acgtcctgct tgtcgcgtac gacatgcacc cggtcgccgg
     9361 tcggccaggt ggtcacccca ccggccctgg ccacggtgac ccgctcggga tcgggctcgg
     9421 cgcccgtcac ccccagctcc gcggtgagcc gggcgcccgc ggcgatgtac tcggcggggc
     9481 cgagacccga cgcgtcgagg gaagcgagct cccggtaccg ggcgcacagc ggctggtcac
     9541 ccatgcggtc cagctgcatg ggcagccact tcggctccag ataggtctcc ccccgccccg
     9601 ccgcccagga cttcacggcc tggagcaacc cggcttcggt ccatgccgcg gcctcctcgt
     9661 cctggccgag ttcccgcagg gcgagcgcgt ggcggatcga ctggcgggcg tggtgtgccc
     9721 accactcgcg catcgtgtcc tggaagtcct cgaaggacat cagccccttg accttcgcca
     9781 ccaggtccgg attgcgcagc gggaagctgc gcagcaggcg ctgacagcgg ttcagcaggt
     9841 cttcggagag cgcgcccggg tggccgtggg tggcggacgt gaccgaggag aactgctcgg
     9901 tgagctgccg tacggagcgg gtcctgacct cgacccgccg cccgtccagg aacaggagcg
     9961 agggcatggt gggcaggtcg gcgtcgctgt cggcgatgag caggaagtcg atgtcggacg
    10021 atgtgttgcc gaagccctcg gcgatcgaac cctccaggac cacggcacag ttggcggctt
    10081 cccggatccg cgggatggtc tggtcgagca gattctgtag atggtcctcg ctgaggatca
    10141 cacgggcctc ccggtcgtga catgggccgt cccgaggacg cgtacgtctt tgagacggcc
    10201 cgctcgcagc tgttcccaca tgtggcgacg tctcggcttg ccgctggacg tctttctgat
    10261 cagcccgctg tgtccgatga cgacggtgat ggcgatgtcg tcgccgagcg cgccgcgcag
    10321 ggcgaccgtg gcgtcctcca cccacgcgcc ctcggccttc tccgcgaaca gcgtcaccga
    10381 ggtgccaccg gtgccctcga cgccgacgac cgcgcacctc tcccgggcga tcccggtggc
    10441 ggagaccacc ttggcctcca ggtcctccat gtagacgctg cggccgcgtg ccttgaggct
    10501 gtcgcccatc cggccgagca cgaacagctc gccacggtgg aagaacccgg cgtccccggt
    10561 cctgatgccg ccttcggtga accgcgtgga gccggtgcgg ccggcgtggt agccactggt
    10621 gaccgagggg ccggtcacct cgatctcacc gaggtgaccg tccggcaggg gccgtccccc
    10681 gtcgtcgacc accctggcgc ccaccccgtc gccggggccg ccgcagccga tcagccagcc
    10741 gtcgccgtcc ccttcaccat ccctgcccac ttcgccatcc ccgccgcctt cgccgtcgcc
    10801 gtcggagggc gtatgcgcgg agctcgcgaa ctcgccctcc ccgtcgatgg cgacgggccc
    10861 cccgaagcgc agcgagcgcg tgtccacgcg tacggtcggc gcgggagtgc cggaggtacg
    10921 tacggtcacc gcgagcgtcg cctcggcgag cccgtacgac ggaaggtaga cggcggggga
    10981 gaatccggcg ggcgct
//
