GCF_002803145.1 - 30 region(s)

Identified secondary metabolite regions using strictness 'relaxed'

NZ_NNBO01000001.1 (Kitasatospora sp. CB02891)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1.1	lanthipeptide-class-iii	35,092	57,599
Region 1.2	T2PKS,NAPAA	220,220	313,004	Low	formicamycin A/formicamycin B/formicamycin C/formicamycin D/formicamycin E/formicamycin F/formicamycin G/formicamycin H/formicamycin I/formicamycin J/formicamycin K/formicamycin L/formicamycin M/fasamycin C/fasamycin D/fasamycin E	PKS:Type II aromatic
Region 1.3	terpene,T3PKS,t3nrps-iterative	708,231	780,082
Region 1.4	thioamitides	795,364	817,225
Region 1.5	hydrogen-cyanide	822,616	835,571
Region 1.6	arylpolyene	919,371	960,474

NZ_NNBO01000010.1 (Kitasatospora sp. CB02891)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 2.1	terpene	58,673	79,857

NZ_NNBO01000011.1 (Kitasatospora sp. CB02891)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 3.1	azoxy-crosslink,T1PKS	99,761	144,483
Region 3.2	lanthipeptide-class-i,terpene-precursor	216,879	248,670

NZ_NNBO01000014.1 (Kitasatospora sp. CB02891)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 6.1	azoxy-crosslink	58,587	86,432

NZ_NNBO01000018.1 (Kitasatospora sp. CB02891)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 10.1	NRPS-like,NRPS	5,112	54,259	Low	stlassin	ribosomal:RiPP

NZ_NNBO01000002.1 (Kitasatospora sp. CB02891)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 12.1	T1PKS,PKS-like,NRPS	104,687	202,784	Medium	meridamycin	NRPS:Type I+PKS
Region 12.2	lanthipeptide-class-iii	234,817	262,150
Region 12.3	lanthipeptide-class-iv	425,758	448,280	High	class IV lanthipeptide/SflA	ribosomal:RiPP

NZ_NNBO01000020.1 (Kitasatospora sp. CB02891)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 13.1	RiPP-like	71,069	82,347

NZ_NNBO01000021.1 (Kitasatospora sp. CB02891)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 14.1	NI-siderophore	46,298	79,742

NZ_NNBO01000022.1 (Kitasatospora sp. CB02891)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 15.1	t3nrps-iterative,NI-siderophore,NRPS	1	72,026

NZ_NNBO01000003.1 (Kitasatospora sp. CB02891)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 23.1	lanthipeptide-class-iv	283,428	306,241
Region 23.2	terpene-precursor	505,073	526,179

NZ_NNBO01000004.1 (Kitasatospora sp. CB02891)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 28.1	T2PKS,T1PKS,PKS-like,NRPS-like,NRPS,lanthipeptide-class-iii,t3nrps-iterative,aminopolycarboxylic-acid	71,462	306,145	Low	LL-D49194α1 (LLD)/LL-D49194β2	PKS

NZ_NNBO01000005.1 (Kitasatospora sp. CB02891)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 29.1	azole-containing-RiPP	7,774	42,598
Region 29.2	transAT-PKS,NRPS,T1PKS	247,603	417,137	Low	leinamycin	NRPS:Type I+PKS:Type I
Region 29.3	terpene	459,866	482,685

NZ_NNBO01000006.1 (Kitasatospora sp. CB02891)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 30.1	terpene	16,703	37,746
Region 30.2	terpene,T3PKS	41,294	103,121	Medium	hopene	terpene
Region 30.3	RiPP-like	175,458	186,258

NZ_NNBO01000008.1 (Kitasatospora sp. CB02891)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 32.1	terpene	46,429	68,621	High	geosmin	terpene:Sesquiterpene

NZ_NNBO01000009.1 (Kitasatospora sp. CB02891)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 33.1	NAPAA,lanthipeptide-class-iv,lanthipeptide-class-ii	95,945	136,640	High	ε-Poly-L-lysine	NRPS:Type I
Region 33.2	terpene	223,746	244,705
Region 33.3	redox-cofactor,triceptide	319,658	341,796	Low	lankacidin C	NRPS:Type I+PKS

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1.1	lanthipeptide-class-iii	35,092	57,599
Region 1.2	T2PKS,NAPAA	220,220	313,004	Low	formicamycin A/formicamycin B/formicamycin C/formicamycin D/formicamycin E/formicamycin F/formicamycin G/formicamycin H/formicamycin I/formicamycin J/formicamycin K/formicamycin L/formicamycin M/fasamycin C/fasamycin D/fasamycin E	PKS:Type II aromatic
Region 1.3	terpene,T3PKS,t3nrps-iterative	708,231	780,082
Region 1.4	thioamitides	795,364	817,225
Region 1.5	hydrogen-cyanide	822,616	835,571
Region 1.6	arylpolyene	919,371	960,474
Region 2.1	terpene	58,673	79,857
Region 3.1	azoxy-crosslink,T1PKS	99,761	144,483
Region 3.2	lanthipeptide-class-i,terpene-precursor	216,879	248,670
Region 6.1	azoxy-crosslink	58,587	86,432
Region 10.1	NRPS-like,NRPS	5,112	54,259	Low	stlassin	ribosomal:RiPP
Region 12.1	T1PKS,PKS-like,NRPS	104,687	202,784	Medium	meridamycin	NRPS:Type I+PKS
Region 12.2	lanthipeptide-class-iii	234,817	262,150
Region 12.3	lanthipeptide-class-iv	425,758	448,280	High	class IV lanthipeptide/SflA	ribosomal:RiPP
Region 13.1	RiPP-like	71,069	82,347
Region 14.1	NI-siderophore	46,298	79,742
Region 15.1	t3nrps-iterative,NI-siderophore,NRPS	1	72,026
Region 23.1	lanthipeptide-class-iv	283,428	306,241
Region 23.2	terpene-precursor	505,073	526,179
Region 28.1	T2PKS,T1PKS,PKS-like,NRPS-like,NRPS,lanthipeptide-class-iii,t3nrps-iterative,aminopolycarboxylic-acid	71,462	306,145	Low	LL-D49194α1 (LLD)/LL-D49194β2	PKS
Region 29.1	azole-containing-RiPP	7,774	42,598
Region 29.2	transAT-PKS,NRPS,T1PKS	247,603	417,137	Low	leinamycin	NRPS:Type I+PKS:Type I
Region 29.3	terpene	459,866	482,685
Region 30.1	terpene	16,703	37,746
Region 30.2	terpene,T3PKS	41,294	103,121	Medium	hopene	terpene
Region 30.3	RiPP-like	175,458	186,258
Region 32.1	terpene	46,429	68,621	High	geosmin	terpene:Sesquiterpene
Region 33.1	NAPAA,lanthipeptide-class-iv,lanthipeptide-class-ii	95,945	136,640	High	ε-Poly-L-lysine	NRPS:Type I
Region 33.2	terpene	223,746	244,705
Region 33.3	redox-cofactor,triceptide	319,658	341,796	Low	lankacidin C	NRPS:Type I+PKS

No secondary metabolite regions were found in these records:: NZ_NNBO01000012.1 (Kitasatospora sp. CB02891); NZ_NNBO01000013.1 (Kitasatospora sp. CB02891); NZ_NNBO01000015.1 (Kitasatospora sp. CB02891); NZ_NNBO01000016.1 (Kitasatospora sp. CB02891); NZ_NNBO01000017.1 (Kitasatospora sp. CB02891); NZ_NNBO01000019.1 (Kitasatospora sp. CB02891); NZ_NNBO01000023.1 (Kitasatospora sp. CB02891); NZ_NNBO01000024.1 (Kitasatospora sp. CB02891); NZ_NNBO01000025.1 (Kitasatospora sp. CB02891); NZ_NNBO01000026.1 (Kitasatospora sp. CB02891); NZ_NNBO01000027.1 (Kitasatospora sp. CB02891); NZ_NNBO01000028.1 (Kitasatospora sp. CB02891); NZ_NNBO01000029.1 (Kitasatospora sp. CB02891); NZ_NNBO01000030.1 (Kitasatospora sp. CB02891); NZ_NNBO01000031.1 (Kitasatospora sp. CB02891); NZ_NNBO01000032.1 (Kitasatospora sp. CB02891); NZ_NNBO01000033.1 (Kitasatospora sp. CB02891); NZ_NNBO01000007.1 (Kitasatospora sp. CB02891)

Compact view

NZ_NNBO01000001.1 - Region 1 - lanthipeptide-class-iii

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Location: 35,092 - 57,599 nt. (total: 22,508 nt) Show pHMM detection rules used

lanthipeptide-class-iii: (micKC and not PKS_KS)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

CG736_RS00215: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acetyltransferase

CG736_RS00195: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000551.5	0.62	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0000513.3	0.62	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0000496.5	0.60	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000519.4	0.58	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0000501.3	0.58	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0000553.3	0.52	ribosomal	SRO15-2212	Streptomyces filamentosus NRRL 15998
BGC0000518.3	0.51	ribosomal	informatipeptin	Streptomyces viridochromogenes DSM 40736
BGC0000528.5	0.46	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0000546.3	0.45	ribosomal	SAL-2242	Streptomyces albidoflavus
BGC0000504.5	0.43	ribosomal	cytolysin ClyLl, cytolysin ClyLs	Plasmid pAD1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000551.5	0.62	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0000513.3	0.62	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0000496.5	0.60	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000519.4	0.58	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0000501.3	0.58	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0000553.3	0.52	ribosomal	SRO15-2212	Streptomyces filamentosus NRRL 15998
BGC0000518.3	0.51	ribosomal	informatipeptin	Streptomyces viridochromogenes DSM 40736
BGC0000528.5	0.46	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0000546.3	0.45	ribosomal	SAL-2242	Streptomyces albidoflavus
BGC0000504.5	0.43	ribosomal	cytolysin ClyLl, cytolysin ClyLs	Plasmid pAD1

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for CG736_RS00220

CG736_RS00225

Leader:

MAILDLQALETPEETTEYR

Core:

SVLSSLSVVNCTNSTVSTLLCL

Core with dehydrations:

DhaVLDhaDhaLDhaVVNCysDhbNDhaDhbVDhaDhbLLCysL

antiSMASH-DB 4.0 matches for CG736_RS00225

91.7%

NC_013929.1and 7 others:

NZ_CP043956.1 Lanthipeptides: F3K20_RS40780_lanthipeptideNZ_CP050692.1 Lanthipeptides: HCX60_RS01560_lanthipeptideNZ_CP119143.1 Lanthipeptides: PYR72_RS02035_lanthipeptideNZ_FWZW01000014.1 Lanthipeptides: B9O01_RS36545_lanthipeptideNZ_KK853997.1 Lanthipeptides: KCH_RS42000_lanthipeptideNZ_KL997500.1 Lanthipeptides: IQ61_RS45670_lanthipeptideNZ_NNBO01000001.1 Lanthipeptides: CG736_RS00225_lanthipeptide

Lanthipeptides: SCAB_RS45825_lanthipeptide

1

SVLSSLSVVNCTNSTVSTLLCL

22

SVLSSLSVVNCTNSTVSTLLCL

3

SVLSSLSVVNCTNSTVSTLLCL

24

90.9%

NZ_BBYG01000014.1and 4 others:

NZ_BMSI01000005.1 Lanthipeptides: IE189_RS14480_lanthipeptideNZ_BMSI01000005.1 Lanthipeptides: IE189_RS14480_lanthipeptideNZ_KB911702.1 Lanthipeptides: H294_RS47360_lanthipeptideNZ_LMEQ01000030.1 Lanthipeptides: ASD48_RS43705_lanthipeptide

Lanthipeptides: AOQ37_RS39920_lanthipeptide

2

VLSSLSVVNCTNSTVSTLLCL

22

VLSSLS VNCTNSTVSTLLCL

1

VLSSLSAVNCTNSTVSTLLCL

21

90.9%

NZ_CP048405.1:

Lanthipeptides: GXW83_RS16365_lanthipeptide

1

SVLSSLSVVNCTNSTVSTLLCL

22

SVLS+LS VNCTNSTVSTLLCL

1

SVLSTLSAVNCTNSTVSTLLCL

22

with 27 more sequence matches

Only the first 10 are displayed here.

87.5%

NZ_CP030772.1and 3 others:

NZ_CP045643.1 Lanthipeptides: GFH48_RS36070_lanthipeptideNZ_CP050975.1 Lanthipeptides: HEP85_RS04970_lanthipeptideNZ_KQ948763.1 Lanthipeptides: AQJ64_RS47660_lanthipeptide

Lanthipeptides: C9F11_RS46215_lanthipeptide

1

SVLSSLSVVNCTNSTVSTLLCL

22

SVLSSLS VNCTNSTVSTLLCL

3

SVLSSLSAVNCTNSTVSTLLCL

24

83.3%

NZ_CP023689.1and 4 others:

NZ_CP056041.1 Lanthipeptides: HUT05_RS01780_lanthipeptideNZ_CP106795.1 Lanthipeptides: N8I86_RS01600_lanthipeptideNZ_KQ949024.1 Lanthipeptides: AQJ58_RS42360_lanthipeptideNZ_LIWA01000005.1 Lanthipeptides: AMK32_RS41535_lanthipeptide

Lanthipeptides: CP983_RS00340_lanthipeptide

1

SVLSSLSVVNCTNSTVSTLLCL

22

SV+SSLS VNCTNSTVSTLLCL

3

SVISSLSAVNCTNSTVSTLLCL

24

63.0%

NZ_NDXL01000001.1:

Lanthipeptides: B7024_RS17090_lanthipeptide

4

SSLSVVNCTNSTVSTLLCL

22

SSLSV+NCT STVSTLLCL

9

SSLSVLNCTVSTVSTLLCL

27

59.3%

NZ_CP054919.1and 1 other:

NZ_JAGIYX010000006.1 Lanthipeptides: J5Y04_RS12150_lanthipeptide

Lanthipeptides: HUT16_RS18950_lanthipeptide

4

SSLSVVNCTNSTVSTLLCL

22

SSLSV+NC STVSTLLCL

9

SSLSVLNCGTSTVSTLLCL

27

50.0%

NZ_CP103871.1:

Lanthipeptides: NYE86_RS07965_lanthipeptide

4

SSLSVVNCTNSTVSTLLCL

22

SSLS++ C S+VS L+CL

3

SSLSLIGCI-SSVSFLVCL

20

50.0%

NZ_FKJI03000003.1:

Lanthipeptides: A7P67_RS08715_lanthipeptide

5

SLSVVNCTNSTVSTLLCL

22

+LS+++C S VSTLLCL

1

TLSLLSCV-SAVSTLLCL

17

45.5%

NZ_JAMTCR010000003.1:

Lanthipeptides: LV79_RS18340_lanthipeptide

4

SSLSVVNCTNSTVSTLLC

21

S+LS V C+ S +S LLC

3

SNLSAVTCSESALSLLLC

20

40.9%

NZ_AAGP01000053.1and 2 others:

NZ_CP004350.1 Lanthipeptides: allorf_2525304_2525438_lanthipeptideNZ_CP025330.1 Lanthipeptides: allorf_3193265_3193399_lanthipeptide

Lanthipeptides: allorf_17179_17313_lanthipeptide

3

LSSLSVVNCTNSTVSTLLC

21

+S+ S+ +CT ST+S L C

2

ISTSSLFSCTESTISFLNC

20

40.0%

NZ_CP022753.1and 1 other:

NZ_CP022753.1 Lanthipeptides: CDO52_RS14300_lanthipeptide

Lanthipeptides: CDO52_RS14300_lanthipeptide

4

SSLSVVNCTN-STVSTLLC

21

S LSV+ C S +S LLC

6

SRLSVITCGGLSQLSALLC

24

39.1%

NZ_CP059557.1:

Lanthipeptides: H2Q94_RS04010_lanthipeptide

8

VVNCTNSTVSTLLC

21

+V C NSTVS L C

9

LVACANSTVSLLTC

22

MIBiG 4.0 matches for CG736_RS00225

36.4%

BGC0000553.3 (SSGG_RS09925):

Lanthipeptide: SRO15-2212

4

SSLSVVNCT

12

SSLSVV CT

13

SSLSVVLCT

21

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.28 out of 26.85

Confidence: medium

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 21.40 out of 27.55

Confidence: medium

Additional weak hits (6):

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.88 out of 25.75

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.07 out of 28.14

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 15.30 out of 29.38

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 14.04 out of 21.55

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 17.86 out of 24.78

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.17 out of 24.78

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

CG736_RS00225 - Class III

Cleavage pHMM score: 13.40

RODEO score: 0

Monoisotopic mass: 2077.1 Da

Molecular weight: 2078.4 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 2096.4 Da N = 1; 2114.5 Da N = 2; 2132.5 Da N = 3; 2150.5 Da N = 4; 2168.5 Da N = 5; 2186.5 Da N = 6; 2204.6 Da N = 7

TFBS definitions

Regulator	Description
GnfM	Regulator of nitrogen-fixation genes
RutR	Regulator of the pyrimidine and purine metabolism

NZ_NNBO01000001.1 - Region 2 - NAPAA,T2PKS

Download region GenBank file

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Location: 220,220 - 313,004 nt. (total: 92,785 nt) Show pHMM detection rules used

T2PKS: (t2ks and t2clf)
NAPAA: (AMP-binding and TIGR02353)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

CG736_RS01275: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

CG736_RS01335: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

CG736_RS01415: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

CG736_RS01460: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

CG736_RS01480: alcohol dehydrogenase

smcogs: SMCOG1040: alcohol dehydrogenase

Transferases: carboxylase methyltransferase transferase

CG736_RS01160: methyltransferase

smcogs: SMCOG1089: methyltransferase

CG736_RS01220: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

CG736_RS01225: acetyl-CoA carboxylase biotin carboxylase

smcogs: SMCOG1090: acetyl-CoA carboxylase biotin carboxylase

CG736_RS01235: acetyl-CoA carboxylase, carboxyl transferase,

smcogs: SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

CG736_RS01340: acetyl-CoA carboxylase biotin carboxylase

smcogs: SMCOG1090: acetyl-CoA carboxylase biotin carboxylase

CG736_RS01345: acetyl-CoA carboxylase, carboxyl transferase,

smcogs: SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

Hydrolases: phosphatase

CG736_RS01330: inositol monophosphatase

smcogs: SMCOG1207: inositol monophosphatase

Lyases: cyclase hydratase

CG736_RS01105: Nitrilase/cyanide hydratase and apolipoprotein

smcogs: SMCOG1294: Nitrilase/cyanide hydratase and apolipoprotein

CG736_RS01240: Polyketide synthesis cyclase

smcogs: SMCOG1225: Polyketide synthesis cyclase

CG736_RS01245: cyclase/dehydrase

smcogs: SMCOG1128: cyclase/dehydrase

CG736_RS01440: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Ligases: ligase synthase

CG736_RS01255: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

CG736_RS01260: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

CG736_RS01325: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

CG736_RS01425: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

CG736_RS01435: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

CG736_RS01475: 8-amino-7-oxononanoate synthase

smcogs: SMCOG1109: 8-amino-7-oxononanoate synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002286.3	0.50	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001641.4	0.50	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002174.2	0.48	NRPS	ε-Poly-L-lysine	Epichloe festucae
BGC0000272.3	0.42	PKS	spore pigment	Streptomyces collinus
BGC0000271.5	0.41	PKS	spore pigment	Streptomyces avermitilis
BGC0000215.5	0.36	PKS	curamycin	Streptomyces cyaneus
BGC0001197.5	0.35	PKS	frankiamicin	Frankia sp. EAN1pec
BGC0000131.5	0.33	PKS	pyrrolomycin	Streptomyces sp. UC 11065
BGC0002474.2	0.33	NRPS	agrobactin	Agrobacterium tumefaciens
BGC0001895.4	0.32	PKS	huanglongmycin A, huanglongmycin B, huanglongmycin C	Streptomyces sp. CB09001

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002174.2	0.77	NRPS	ε-Poly-L-lysine	Epichloe festucae
BGC0000272.3	0.73	PKS	spore pigment	Streptomyces collinus
BGC0000271.5	0.73	PKS	spore pigment	Streptomyces avermitilis
BGC0000215.5	0.68	PKS	curamycin	Streptomyces cyaneus
BGC0000234.5	0.65	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0000231.5	0.64	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0002135.2	0.61	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001166.5	0.60	NRPS	HC-toxin	Alternaria jesenskae
BGC0002276.2	0.60	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0001251.4	0.60	NRPS	epichloenin A	Epichloe festucae

Similar known clusters from MIBiG 4.0

Similar subclusters

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 19.46 out of 20.47

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.71 out of 23.79

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.37 out of 20.47

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.39 out of 19.26

Confidence: strong

DosR

Description: Hypoxia stress response regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 24.07 out of 29.51

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.46 out of 23.05

Confidence: medium

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 17.23 out of 19.52

Confidence: medium

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 22.05 out of 27.04

Confidence: medium

Additional weak hits (29):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 18.99 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.44 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.89 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.94 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.29 out of 26.06

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.92 out of 28.14

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.17 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.65 out of 29.38

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 22.92 out of 30.22

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.31 out of 26.85

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.57 out of 27.78

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.69 out of 29.38

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.56 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.20 out of 26.06

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.38 out of 26.85

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 14.82 out of 21.55

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.29 out of 18.57

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.46 out of 39.62

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.88 out of 25.75

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.19 out of 18.57

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.75 out of 27.78

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.40 out of 21.84

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.40 out of 21.84

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.57 out of 33.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.52 out of 28.19

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.31 out of 33.14

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.08 out of 35.63

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 19.22 out of 23.43

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.14 out of 35.63

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Type II PKS

TFBS Finder

Predicted core structure(s)

For candidate cluster 2, location 220219 - 313004:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 276437 - 313004:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

CG736_RS01325: X

AMP-binding (28..428): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: NQVLHIYGLTARYVLSAAALQAYGMTELSPATHI
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

CG736_RS01425: Pro

Search NORINE for peptide: strict or relaxed

AMP-binding (25..426): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: ITPTHLAGVLSVWVMYGGAVGVYGMSEAQMISAL
Nearest Stachelhaus code(s):: Val DALWMGGVFK (35% 8Å match)
Stachelhaus code match:: 60% (weak)

CG736_RS01435: X

AMP-binding (40..412): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FAELFDASCEELSTIVGGEWNTYGPTETTVVATA
Nearest Stachelhaus code(s):: Lys DAEDIGTVVK (71% 8Å match); Gln or Tyr DAESVGTVVK (59% 8Å match)
Stachelhaus code match:: 90% (moderate)

Link to PARAS substrate predictor

Type II PKS gene cluster prediction details

Cluster section:: 220219 to 292702
Starter unit(s):: acetyl-CoA (Score: 0.0; E-value: 0)
Product class(es):: pentangular polyphenol
tetracenomycin

CG736_RS01220: MET C6|C8 (Score: 497.3; E-value: 4e-152)
CG736_RS01240: CYC C4-C21/C2-C23|C2-C19 (Score: 144.6; E-value: 2.1e-45)
CG736_RS01245: CYC C9-C14 (Score: 238.3; E-value: 6.2e-74)
CG736_RS01250: ACP (Score: 44.0; E-value: 4e-14)
CG736_RS01255: CLF (Score: 539.1; E-value: 1.5e-164)
CG736_RS01260: KS (Score: 714.5; E-value: 8.6e-218)
CG736_RS01265: CYC C5-C18 (Score: 207.8; E-value: 1.1e-64)
CG736_RS01275: OXY (Score: 277.7; E-value: 4.5e-85)
CG736_RS01415: KR (Score: 157.7; E-value: 9.8e-49)

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
AfsR	Pleiotropic regulatory for antibiotic production
CRP	Cyclic AMP receptor protein
DasR	N-acetylglucosamine dependent repressor
DosR	Hypoxia stress response regulator
IolR	Regulator of the inositol catabolism
VqsM	Regulator of quorum-sensing signalling systems

NZ_NNBO01000001.1 - Region 3 - T3PKS,t3nrps-iterative,terpene

Download region GenBank file

Download region SVG

Location: 708,231 - 780,082 nt. (total: 71,852 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)
T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)
t3nrps-iterative: (AMP-binding and PP-binding and (DUF98 or Orn_Arg_deC_N or Orn_DAP_Arg_deC) and not ATP-grasp)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxygenase

CG736_RS03420: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

CG736_RS03435: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

Transferases: PPTase aminotransferase glycosyltransferase

CG736_RS03535: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

CG736_RS03550: aminotransferase

smcogs: SMCOG1019: aminotransferase

CG736_RS35885: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1012: 4'-phosphopantetheinyl transferase

Hydrolases: hydrolase

CG736_RS03555: alpha/beta hydrolase domain-containing protein

smcogs: SMCOG1066: alpha/beta hydrolase domain-containing protein

Lyases: cyclase decarboxylase

CG736_RS03430: Terpene synthase/cyclase metal-binding domain protein

smcogs: SMCOG1052: Terpene synthase/cyclase metal-binding domain protein

CG736_RS03620: diaminopimelate decarboxylase

smcogs: SMCOG1264: diaminopimelate decarboxylase

Ligases: ligase synthase synthetase

CG736_RS03455: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

CG736_RS03485: hydroxymethylglutaryl-CoA synthase

smcogs: SMCOG1043: hydroxymethylglutaryl-CoA synthase

CG736_RS03525: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

CG736_RS03565: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

CG736_RS03580: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

CG736_RS03590: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

CG736_RS03595: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

CG736_RS03615: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000286.3	0.93	PKS	viguiepinol	Streptomyces sp. KO-3988
BGC0002561.3	0.65	other (other)	phenazine SA, phenazine SB, phenazine SC	Streptomyces sp.
BGC0000673.3	0.64	terpene	pimara-8(14),15-diene	Aspergillus nidulans FGSC A4
BGC0001080.5	0.63	other (phenazine)	endophenazine A, endophenazine B	Streptomyces anulatus
BGC0000934.4	0.62	other (phenazine)	5-acetyl-5,10-dihydrophenazine-1-carboxylic acid, 5-(2-hydroxyacetyl)-5,10-dihydrophenazine-1-carboxylic acid, endophenazine A1, endophenazine F, endophenazine G	Kitasatospora sp. HKI 714
BGC0000677.5	0.59	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0001078.5	0.59	PKS, terpene	furaquinocin B	Streptomyces sp. KO-3988
BGC0000675.5	0.54	terpene	(+)-T-muurolol	Streptomyces clavuligerus ATCC 27064
BGC0002309.2	0.53	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0001911.3	0.53	terpene	raimonol	Streptomyces anulatus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000286.3	0.66	PKS	viguiepinol	Streptomyces sp. KO-3988
BGC0002561.3	0.56	other (other)	phenazine SA, phenazine SB, phenazine SC	Streptomyces sp.
BGC0001080.5	0.55	other (phenazine)	endophenazine A, endophenazine B	Streptomyces anulatus
BGC0001160.5	0.55	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0000673.3	0.54	terpene	pimara-8(14),15-diene	Aspergillus nidulans FGSC A4
BGC0001078.5	0.54	PKS, terpene	furaquinocin B	Streptomyces sp. KO-3988
BGC0000934.4	0.53	other (phenazine)	5-acetyl-5,10-dihydrophenazine-1-carboxylic acid, 5-(2-hydroxyacetyl)-5,10-dihydrophenazine-1-carboxylic acid, endophenazine A1, endophenazine F, endophenazine G	Kitasatospora sp. HKI 714
BGC0000677.5	0.53	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0001529.5	0.52	other (other)	benzastatin J, 7-hydroxyl benzastatin B, 7-hydroxyl O-demethylbenzastatin A, JBIR-67, 7-hydroxyl benzastatin F, 7-hydroxyl O-demethylbenzastatin D, O-demethylvirantmycin, 7-hydroxyl benzastatin D, virantmycin, benzastatin K, methylbenzastatin K	Streptomyces sp. RI18
BGC0000679.5	0.51	terpene	diazepinomicin	Micromonospora sp. M42

Similar known clusters from MIBiG 4.0

Similar subclusters

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
diterpene			C20	C6-C11 + C10-C15	PP

Genes with terpene domains:

CG736_RS03425: T2TS

Domain 1 (4 - 505): Type 2 terpene synthase

Subtype: Diterpene synthase

Reactions:: GGPP → diterpene_C6-C11/C10-C15_PP

CG736_RS03430: T1TS

Domain 1 (10 - 310): Type 1 terpene synthase

Subtype: unknown

CG736_RS03455: PT_FPPS_like

Domain 1 (63 - 346): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 21.28 out of 26.33

Confidence: medium

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 20.54 out of 26.33

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.52 out of 19.26

Confidence: medium

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 23.00 out of 27.04

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.80 out of 20.47

Confidence: medium

Additional weak hits (27):

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.53 out of 30.25

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.12 out of 18.57

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.80 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.61 out of 28.14

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 22.22 out of 30.22

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 13.37 out of 18.57

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 16.48 out of 29.25

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.72 out of 20.97

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.87 out of 27.78

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.29 out of 39.62

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.30 out of 21.84

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.56 out of 30.25

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 20.58 out of 27.55

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.76 out of 30.25

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.03 out of 29.38

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.59 out of 26.06

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.42 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.78 out of 28.19

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 20.75 out of 27.04

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.56 out of 29.65

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 19.87 out of 29.65

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 17.62 out of 24.78

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.90 out of 32.93

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.94 out of 22.40

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.01 out of 26.06

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 26.89 out of 39.62

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 17.93 out of 33.14

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

Terpene

TFBS Finder

NRPS/PKS substrate predictions

CG736_RS03565: X

AMP-binding (46..383): X

nrpys: Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: ECYSH---VLGGIGIVAGEGTMYGMTELGVIATD
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

CG736_RS03590: X

AMP-binding (46..432): X

nrpys: Phe, Trp, Ph-Gly, Tyr, bOH-Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: SHRLHAATLIALGFWNTGD-----------TVTR
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

Link to PARAS substrate predictor

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
ANR	Anaerobic transcriptional regulator
AbrC3	Antibiotic production activator
CRP	Cyclic AMP receptor protein
DasR	N-acetylglucosamine dependent repressor
VqsM	Regulator of quorum-sensing signalling systems

NZ_NNBO01000001.1 - Region 4 - thioamitides

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Location: 795,364 - 817,225 nt. (total: 21,862 nt) Show pHMM detection rules used

thioamitides: ((YcaO or TIGR03604) and TfuA)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

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Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase

CG736_RS03880: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

Transferases: acetyltransferase

CG736_RS03885: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

Ligases: synthase

CG736_RS03870: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002007.2	0.49	terpene	atolypene A, atolypene B	Amycolatopsis tolypomycina
BGC0001849.4	0.48	ribosomal	thiovarsolin A, thiovarsolin B, thiovarsolin C, thiovarsolin D	Streptomyces varsoviensis
BGC0000516.4	0.46	ribosomal	geobacillin II	Geobacillus thermodenitrificans
BGC0000665.5	0.42	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0002669.2	0.42	ribosomal	TVA-YJ-2	Streptomyces sp. NRRL S-87
BGC0002670.2	0.41	other (other)	fosfonochlorin	Fusarium oxysporum f. sp. lycopersici 4287
BGC0001555.4	0.41	ribosomal	colicin V	Escherichia coli chi7122
BGC0000843.5	0.41	other (non-nrp beta-lactam)	alanylclavam, 2-hydroxymethylclavam, 2-formyloxymethylclavam, clavam-2-carboxylate	Streptomyces clavuligerus ATCC 27064
BGC0002316.2	0.41	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0000584.5	0.40	ribosomal	colicin V	Escherichia coli

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002007.2	0.49	terpene	atolypene A, atolypene B	Amycolatopsis tolypomycina
BGC0001849.4	0.48	ribosomal	thiovarsolin A, thiovarsolin B, thiovarsolin C, thiovarsolin D	Streptomyces varsoviensis
BGC0000516.4	0.46	ribosomal	geobacillin II	Geobacillus thermodenitrificans
BGC0000665.5	0.42	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0002669.2	0.42	ribosomal	TVA-YJ-2	Streptomyces sp. NRRL S-87
BGC0002670.2	0.41	other (other)	fosfonochlorin	Fusarium oxysporum f. sp. lycopersici 4287
BGC0001555.4	0.41	ribosomal	colicin V	Escherichia coli chi7122
BGC0000843.5	0.41	other (non-nrp beta-lactam)	alanylclavam, 2-hydroxymethylclavam, 2-formyloxymethylclavam, clavam-2-carboxylate	Streptomyces clavuligerus ATCC 27064
BGC0002316.2	0.41	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0000584.5	0.40	ribosomal	colicin V	Escherichia coli

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 19.35 out of 20.47

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.19 out of 23.79

Confidence: medium

Additional weak hits (4):

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.00 out of 26.85

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 15.97 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.71 out of 28.14

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 19.21 out of 26.33

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism
VqsM	Regulator of quorum-sensing signalling systems

NZ_NNBO01000001.1 - Region 5 - hydrogen-cyanide

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Location: 822,616 - 835,571 nt. (total: 12,956 nt) Show pHMM detection rules used

hydrogen-cyanide: (Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase reductase

CG736_RS03940: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

CG736_RS03955: alkyl hydroperoxide reductase subunit

smcogs: SMCOG1176: alkyl hydroperoxide reductase subunit

CG736_RS03965: FAD dependent oxidoreductase

smcogs: SMCOG1103: FAD dependent oxidoreductase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002345.2	0.63	other (other)	hydrogen cyanide	Pseudomonas fluorescens
BGC0001424.4	0.33	NRPS, PKS	myxochromide C	Myxococcus virescens
BGC0001427.4	0.33	NRPS, PKS	myxochromide A	Myxococcus xanthus
BGC0001428.4	0.33	NRPS, PKS	myxochromide A	Myxococcus xanthus
BGC0001423.4	0.33	NRPS, PKS	myxochromide C	Myxococcus hansupus
BGC0001421.4	0.33	NRPS, PKS	myxochromide B	Myxococcus sp.
BGC0001422.4	0.32	NRPS, PKS	myxochromide A	Myxococcus fulvus HW-1
BGC0001739.3	0.30	other (other)	phosphonoacetic Acid	Streptomyces sp. NRRL F-525
BGC0001108.5	0.28	NRPS, PKS	pederin, pseudopederin, pederone	Uncultured bacterium
BGC0002556.2	0.28	other (other)	cervinomycin B3, cervinomycin C1, cervinomycin C2, cervinomycin C3, cervinomycin C4	Streptomyces sp.

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002345.2	0.63	other (other)	hydrogen cyanide	Pseudomonas fluorescens
BGC0001424.4	0.33	NRPS, PKS	myxochromide C	Myxococcus virescens
BGC0001427.4	0.33	NRPS, PKS	myxochromide A	Myxococcus xanthus
BGC0001428.4	0.33	NRPS, PKS	myxochromide A	Myxococcus xanthus
BGC0001423.4	0.33	NRPS, PKS	myxochromide C	Myxococcus hansupus
BGC0001421.4	0.33	NRPS, PKS	myxochromide B	Myxococcus sp.
BGC0001422.4	0.32	NRPS, PKS	myxochromide A	Myxococcus fulvus HW-1
BGC0001739.3	0.30	other (other)	phosphonoacetic Acid	Streptomyces sp. NRRL F-525
BGC0001108.5	0.28	NRPS, PKS	pederin, pseudopederin, pederone	Uncultured bacterium
BGC0002556.2	0.28	other (other)	cervinomycin B3, cervinomycin C1, cervinomycin C2, cervinomycin C3, cervinomycin C4	Streptomyces sp.

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.19 out of 23.79

Confidence: medium

Additional weak hits (8):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 17.99 out of 26.33

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 12.38 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 12.70 out of 26.06

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 15.81 out of 29.25

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.74 out of 27.78

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 16.96 out of 22.40

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.40 out of 21.84

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 15.34 out of 33.14

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism

NZ_NNBO01000001.1 - Region 6 - arylpolyene

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Location: 919,371 - 960,474 nt. (total: 41,104 nt) Show pHMM detection rules used

arylpolyene: (APE_KS1 or APE_KS2)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene/CDS overview

Filter:

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: PPTase aminotransferase glycosyltransferase transferase

CG736_RS04375: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1012: 4'-phosphopantetheinyl transferase

CG736_RS04395: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

CG736_RS04410: aminotransferase

smcogs: SMCOG1019: aminotransferase

CG736_RS04500: acetyl-CoA carboxylase, carboxyl transferase,

smcogs: SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

Hydrolases: hydrolase

CG736_RS04415: alpha/beta hydrolase domain-containing protein

smcogs: SMCOG1066: alpha/beta hydrolase domain-containing protein

Ligases: ligase synthase

CG736_RS04365: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

CG736_RS04390: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

CG736_RS04430: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

CG736_RS04445: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

CG736_RS04455: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

CG736_RS04460: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

CG736_RS04480: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002174.2	0.59	NRPS	ε-Poly-L-lysine	Epichloe festucae
BGC0001160.5	0.57	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001535.3	0.55	other (other)	branched-chain fatty acids	Streptomyces filamentosus
BGC0001534.3	0.53	other (other)	branched-chain fatty acids	Streptomyces filamentosus
BGC0001917.4	0.47	PKS	5aTHQ-9i, 5aTHQ-9n, 5aTHQ-10a, STAMs-9i, STAMs-9n	Streptomyces nigrescens
BGC0002711.2	0.47	PKS	nostovalerolactone, 9-dehydronostovalerolactone	Nostoc punctiforme PCC 73102
BGC0002536.3	0.45	NRPS	alazopeptin	Kitasatospora azatica KCTC 9699
BGC0000261.5	0.44	PKS	R1128	Streptomyces sp. R1128
BGC0002034.3	0.44	other (other)	perquinoline A, perquinoline B, perquinoline C	Streptomyces sp. IB2014 016-6
BGC0001987.2	0.44	PKS	koreenceine A, koreenceine B, koreenceine C, koreenceine D	Pseudomonas koreensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002174.2	0.59	NRPS	ε-Poly-L-lysine	Epichloe festucae
BGC0001160.5	0.57	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001535.3	0.55	other (other)	branched-chain fatty acids	Streptomyces filamentosus
BGC0001534.3	0.53	other (other)	branched-chain fatty acids	Streptomyces filamentosus
BGC0001917.4	0.47	PKS	5aTHQ-9i, 5aTHQ-9n, 5aTHQ-10a, STAMs-9i, STAMs-9n	Streptomyces nigrescens
BGC0002711.2	0.47	PKS	nostovalerolactone, 9-dehydronostovalerolactone	Nostoc punctiforme PCC 73102
BGC0002536.3	0.45	NRPS	alazopeptin	Kitasatospora azatica KCTC 9699
BGC0000261.5	0.44	PKS	R1128	Streptomyces sp. R1128
BGC0002034.3	0.44	other (other)	perquinoline A, perquinoline B, perquinoline C	Streptomyces sp. IB2014 016-6
BGC0001987.2	0.44	PKS	koreenceine A, koreenceine B, koreenceine C, koreenceine D	Pseudomonas koreensis

Similar known clusters from MIBiG 4.0

Similar subclusters

Transcription Factor Binding Sites

Binding sites found:

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 19.78 out of 20.97

Confidence: strong

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 22.08 out of 27.04

Confidence: medium

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 21.31 out of 27.04

Confidence: medium

Additional weak hits (11):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.76 out of 26.33

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 18.40 out of 36.54

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.56 out of 24.78

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 20.82 out of 27.04

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 15.76 out of 21.84

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.39 out of 26.85

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.49 out of 28.19

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.57 out of 20.01

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 17.71 out of 36.54

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 15.45 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.68 out of 28.14

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

TFBS Finder

NRPS/PKS substrate predictions

CG736_RS04430: X

AMP-binding (40..375): X

nrpys: Gly, Ala, Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: ECYSHVLGVGGHIGVVAGEGTMYGMTETGVIATD
Nearest Stachelhaus code(s):: Ala DLGIVGGMIK (32% 8Å match)
Stachelhaus code match:: 60% (weak)

CG736_RS04455: X

AMP-binding (48..434): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AHRLHAATLIALWQWNTGD-----------TATR
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
CcpA	Catabolite control protein A
DasR	N-acetylglucosamine dependent repressor

NZ_NNBO01000010.1 - Region 1 - terpene

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Location: 58,673 - 79,857 nt. (total: 21,185 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

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resistance

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Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: methyltransferase

CG736_RS22185: methyltransferase

smcogs: SMCOG1089: methyltransferase

Hydrolases: glucanase

CG736_RS22180: Pullulanase, type I

smcogs: SMCOG1297: Pullulanase, type I

Lyases: cyclase

CG736_RS22190: Terpene synthase/cyclase metal-binding domain protein

smcogs: SMCOG1052: Terpene synthase/cyclase metal-binding domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000657.5	0.70	terpene	2-methylisoborneol	Pseudanabaena sp. dqh15
BGC0000659.3	0.62	terpene	2-methylisoborneol	Saccharopolyspora erythraea NRRL 2338
BGC0000658.3	0.61	terpene	2-methylisoborneol	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000519.4	0.49	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0002150.2	0.43	ribosomal	triculamin	Streptomyces triculaminicus
BGC0002151.2	0.43	ribosomal	alboverticillin	Streptomyces griseocarneus
BGC0000501.3	0.42	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0000551.5	0.41	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0002005.2	0.41	ribosomal	RaxX	Xanthomonas oryzae pv. oryzae
BGC0000584.5	0.41	ribosomal	colicin V	Escherichia coli

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000657.5	0.70	terpene	2-methylisoborneol	Pseudanabaena sp. dqh15
BGC0000659.3	0.62	terpene	2-methylisoborneol	Saccharopolyspora erythraea NRRL 2338
BGC0000658.3	0.61	terpene	2-methylisoborneol	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000519.4	0.49	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0002150.2	0.43	ribosomal	triculamin	Streptomyces triculaminicus
BGC0002151.2	0.43	ribosomal	alboverticillin	Streptomyces griseocarneus
BGC0000501.3	0.42	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0000551.5	0.41	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0002005.2	0.41	ribosomal	RaxX	Xanthomonas oryzae pv. oryzae
BGC0000584.5	0.41	ribosomal	colicin V	Escherichia coli

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
monoterpene		2-methylisoborneol	C11	C1-C6	OH
monoterpene		2-methylenebornane	C11	C1-C6	none

Genes with terpene domains:

CG736_RS22190: T1TS

Domain 1 (41 - 391): Type 1 terpene synthase

Subtype: 2-Methylisoborneol synthase type

Reactions:: 2-MeGPP → 2-methylisoborneol; 2-MeGPP → 2-methylenebornane

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.92 out of 20.47

Confidence: medium

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 28.04 out of 35.63

Confidence: medium

Additional weak hits (2):

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 19.75 out of 23.43

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.58 out of 26.85

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
2-MeGPP	2-methylgeranyl diphosphate

TFBS definitions

Regulator	Description
NrtR	NAD synthesis repressor
VqsM	Regulator of quorum-sensing signalling systems

NZ_NNBO01000011.1 - Region 1 - T1PKS,azoxy-crosslink

Download region GenBank file

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Location: 99,761 - 144,483 nt. (total: 44,723 nt) Show pHMM detection rules used

azoxy-crosslink: (azdH and LPG_synthase_C and azdO and vlmB)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

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resistance

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Gene overview

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NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidase oxidoreductase

CG736_RS23690: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

CG736_RS23710: pyruvate oxidase/decarboxylase

smcogs: SMCOG1055: pyruvate oxidase/decarboxylase

CG736_RS23745: flavin reductase domain protein FMN-binding

smcogs: SMCOG1160: flavin reductase domain protein FMN-binding

CG736_RS23755: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

CG736_RS23805: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: sulfotransferase

CG736_RS23720: Sulfotransferase domain

extras: Sulfotransfer_3: Sulfotransferase domain

Hydrolases: esterase hydrolase

CG736_RS23770: thioesterase

smcogs: SMCOG1004: thioesterase

CG736_RS23800: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Lyases: decarboxylase lyase

CG736_RS23695: argininosuccinate lyase/adenylosuccinate lyase

smcogs: SMCOG1063: argininosuccinate lyase/adenylosuccinate lyase

CG736_RS23760: Decarboxylase, pyridoxal-dependent

smcogs: SMCOG1180: Decarboxylase, pyridoxal-dependent

Ligases: ligase synthase

CG736_RS23705: cysteine synthase

smcogs: SMCOG1081: cysteine synthase

CG736_RS23775: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

CG736_RS23780: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Translocases: permease

CG736_RS23820: phenylalanine-specific permease

smcogs: SMCOG1038: phenylalanine-specific permease

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.63	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.63	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.63	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.62	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002154.2	0.61	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.60	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0001258.3	0.60	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis
BGC0001284.4	0.58	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001160.5	0.56	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0002191.2	0.56	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.66	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.66	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.66	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.66	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001160.5	0.64	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0002154.2	0.64	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001164.3	0.64	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001257.3	0.64	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0001258.3	0.63	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis
BGC0002191.2	0.63	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.26 out of 19.26

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.10 out of 23.79

Confidence: medium

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.99 out of 20.97

Confidence: medium

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 28.92 out of 30.98

Confidence: strong

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 29.48 out of 36.53

Confidence: medium

Additional weak hits (21):

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.92 out of 25.75

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.58 out of 30.25

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.17 out of 28.14

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 16.79 out of 30.97

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 22.39 out of 36.54

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.44 out of 30.25

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.83 out of 26.85

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.55 out of 26.06

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.31 out of 27.78

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.27 out of 26.85

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.07 out of 32.74

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.27 out of 30.25

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.39 out of 32.74

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 18.29 out of 31.73

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 20.36 out of 32.74

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.83 out of 20.97

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.79 out of 20.97

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 23.19 out of 35.63

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.39 out of 28.19

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.59 out of 29.65

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.76 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 99760 - 144483:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 100649 - 144483:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

CG736_RS23775: X

AMP-binding (15..420): X

nrpys: Gly, Ala, Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FNMFHAGGYAWHWAVYGGSVQAYAAAETGSFITC
Nearest Stachelhaus code(s):: Cin AILWVAASGK (29% 8Å match)
Stachelhaus code match:: 60% (weak)

CG736_RS23780: mal

PKS_AT (533..829): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 75.0%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 147.0
inactive: 84.9
Methylmalonyl-CoA: 65.5
Methoxymalonyl-CoA: 59.1
Ethylmalonyl-CoA: 45.0
Propionyl-CoA: 42.1
fatty_acid: 30.0
Isobutyryl-CoA: 29.7
Benzoyl-CoA: 29.6
CHC-CoA: 19.6
Acetyl-CoA: 17.8
2-Methylbutyryl-CoA: 16.5
trans-1,2-CPDA: 15.8
3-Methylbutyryl-CoA: 10.3

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
CcpA	Catabolite control protein A
DmdR1	Iron(II)-dependent repressor
IolR	Regulator of the inositol catabolism
NuR	Nickel-responsive regulator

NZ_NNBO01000011.1 - Region 2 - lanthipeptide-class-i,terpene-precursor

Download region GenBank file

Download region SVG

Location: 216,879 - 248,670 nt. (total: 31,792 nt) Show pHMM detection rules used

lanthipeptide-class-i: (((Lant_dehydr_N or Lant_dehydr_C) and cds(LANC_like and not Pkinase)) and not (YcaO or TIGR03604))
terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Lyases: cyclase dehydratase

CG736_RS24255: Lanthionine synthetase C family protein

smcogs: SMCOG1140: Lanthionine synthetase C family protein

CG736_RS24260: Lantibiotic dehydratase domain protein

smcogs: SMCOG1155: Lantibiotic dehydratase domain protein

CG736_RS24300: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: synthetase

CG736_RS24295: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002576.2	0.89	ribosomal	mechercharmycin	Thermoactinomyces sp.
BGC0000677.5	0.30	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0001289.5	0.29	ribosomal	penisin	Paenibacillus ehimensis
BGC0000657.5	0.29	terpene	2-methylisoborneol	Pseudanabaena sp. dqh15
BGC0002309.2	0.29	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0001911.3	0.26	terpene	raimonol	Streptomyces anulatus
BGC0000642.3	0.26	terpene	carotenoid	Enterobacteriaceae bacterium DC413
BGC0001727.3	0.23	ribosomal	paenilan	Paenibacillus polymyxa E681
BGC0000644.3	0.23	terpene	carotenoid	Dietzia sp. CQ4
BGC0000664.5	0.23	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002576.2	0.75	ribosomal	mechercharmycin	Thermoactinomyces sp.
BGC0001289.5	0.47	ribosomal	penisin	Paenibacillus ehimensis
BGC0002309.2	0.46	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0000677.5	0.45	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0002635.2	0.44	ribosomal	daspyromycin A, daspyromycin B	Actinokineospora diospyrosa
BGC0000657.5	0.44	terpene	2-methylisoborneol	Pseudanabaena sp. dqh15
BGC0000541.5	0.44	ribosomal	paenicidin A	Paenibacillus polymyxa
BGC0000542.3	0.44	ribosomal	paenicidin B	Paenibacillus terrae
BGC0001911.3	0.43	terpene	raimonol	Streptomyces anulatus
BGC0000642.3	0.43	terpene	carotenoid	Enterobacteriaceae bacterium DC413

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for CG736_RS24260

CG736_RS24265

Leader:

MRNEILVQQDTDLDLDLRIAEVAEETQAFGQ

Core:

GTFTSPSSYAIGTRCPVCC

Core with dehydrations:

GDhbFDhbDhaPDhaDhaYAIGDhbRCysPVCysCys

antiSMASH-DB 4.0 matches for CG736_RS24265

100.0%

NZ_NNBO01000011.1:

Lanthipeptides: CG736_RS24265_lanthipeptide

1

GTFTSPSSYAIGTRCPVCC

19

GTFTSPSSYAIGTRCPVCC

1

GTFTSPSSYAIGTRCPVCC

19

78.9%

NZ_KK853997.1:

Lanthipeptides: KCH_RS38645_lanthipeptide

4

TSPSSYAIGTRCPVCC

19

TSPSSYAIGTRCP+CC

1

TSPSSYAIGTRCPICC

16

78.9%

NZ_NNBP01000006.1:

Lanthipeptides: CG747_RS11965_lanthipeptide

4

TSPSSYAIGTRCPVCC

19

TSPSSYAIGTRCP CC

1

TSPSSYAIGTRCPTCC

16

with 10 more sequence matches

75.0%

NZ_BEWD01000004.1and 15 others:

NZ_BMUN01000001.1 Lanthipeptides: IE238_RS00870_lanthipeptideNZ_BMVQ01000001.1 Lanthipeptides: IE283_RS00890_lanthipeptideNZ_BMWI01000001.1 Lanthipeptides: IE253_RS05465_lanthipeptideNZ_CP012382.1 Lanthipeptides: SAM23877_RS36835_lanthipeptideNZ_CP012949.1 Lanthipeptides: SAM40697_RS33360_lanthipeptideNZ_CP027299.1 Lanthipeptides: C1N79_RS34825_lanthipeptideNZ_CP039123.1 Lanthipeptides: E5N77_RS35110_lanthipeptideNZ_CP042324.1 Lanthipeptides: FQ762_RS01285_lanthipeptideNZ_CP104697.1 Lanthipeptides: N6Q81_RS33410_lanthipeptideNZ_CP104697.1 Lanthipeptides: N6Q81_RS33410_lanthipeptideNZ_GG657756.1 Lanthipeptides: SSPG_RS36720_lanthipeptideNZ_JACWAA010000001.1 Lanthipeptides: ICA23_RS00945_lanthipeptideNZ_KZ195574.1 Lanthipeptides: B9W62_RS39030_lanthipeptideNZ_LT629768.1 Lanthipeptides: BLU60_RS01655_lanthipeptideNZ_MQUH01000030.1 Lanthipeptides: BSZ07_RS37165_lanthipeptide

Lanthipeptides: Sru02f_RS16280_lanthipeptide

1

GTFTSPSSYAIGTRCPVCC

19

GTFTSPSSYAIGTRCP+CC

6

GTFTSPSSYAIGTRCPICC

24

75.0%

NZ_CP043959.1and 11 others:

NZ_CP058273.1 Lanthipeptides: AY578_RS00390_lanthipeptideNZ_CP104864.1 Lanthipeptides: N7E53_RS01070_lanthipeptideNZ_CP113837.1 Lanthipeptides: OU787_RS28205_lanthipeptideNZ_JMLN01000003.1 Lanthipeptides: BR33_RS35695_lanthipeptideNZ_KB891308.1 Lanthipeptides: B061_RS37915_lanthipeptideNZ_KL503830.1 Lanthipeptides: DJ64_RS34550_lanthipeptideNZ_KZ819159.1 Lanthipeptides: DBP19_RS30055_lanthipeptideNZ_LISX01000001.1 Lanthipeptides: AMK19_RS02530_lanthipeptideNZ_RCOL01000001.1 Lanthipeptides: STAN_RS32200_lanthipeptideNZ_VIUV01000001.1 Lanthipeptides: FB570_RS00565_lanthipeptideNZ_WUUF01000042.1 Lanthipeptides: GSE51_RS09870_lanthipeptide

Lanthipeptides: F3L20_RS18420_lanthipeptide

1

GTFTSPSSYAIGTRCPVCC

19

GT+TSPSSYAIGTRCPVCC

6

GTYTSPSSYAIGTRCPVCC

24

70.8%

NZ_CP054939.1and 2 others:

NZ_FKJH01000076.1 Lanthipeptides: STEPF7_RS31605_lanthipeptideNZ_GG657758.1 Lanthipeptides: SSRG_RS36710_lanthipeptide

Lanthipeptides: HUT14_RS33610_lanthipeptide

1

GTFTSPSSYAIGTRCPVCC

19

GT+TSPSSYAIGTRCP+CC

6

GTYTSPSSYAIGTRCPICC

24

47.6%

NZ_CP050693.1:

Lanthipeptides: HC362_RS33495_lanthipeptide

1

GTFTSPSSYAIGTRCPVCC

19

G +TSPS+Y G+ C +CC

4

GGYTSPSTY--GSPCNLCC

20

29.6%

NZ_BIFH01000040.1:

Lanthipeptides: EMA17_RS40630_lanthipeptide

5

SPSSYAIGTRCPVCC

19

SPS+ + +RC VCC

12

SPSTRGVQSRCWVCC

26

27.0%

NZ_GG657758.1:

Lanthipeptides: SSRG_RS00800_lanthipeptide

1

GTFTSPSSYAIGTRCPVCC

19

G TSPS+Y G+ C +CC

1

GGATSPSTY--GSPCNLCC

17

26.1%

NZ_AP018498.1:

Lassopeptides: AEB_RS18125_lassopeptide

4

TSPSSYAIG

12

T PS YA+G

11

TGPSRYAMG

19

19.4%

NZ_CP086141.1and 1 other:

NZ_CP086141.1 Lanthipeptides: LL065_RS24800_lanthipeptide

Lanthipeptides: LL065_RS24800_lanthipeptide

1

GTFTSPSSYAIGTRCPVC

18

GT +SYA+G + +C

9

GTVGCLASYALGNQGKIC

26

15.8%

NZ_LT575476.1and 1 other:

NZ_LT575476.1 Lanthipeptides: BN6628_RS02285_lanthipeptide

Lanthipeptides: BN6628_RS02285_lanthipeptide

7

SSYAIGTRCPVC

18

+SYA+G + VC

15

ASYALGDKGQVC

26

15.0%

NZ_FQZK01000006.1and 1 other:

NZ_FQZK01000006.1 Lanthipeptides: BUE45_RS13115_lanthipeptide

Lanthipeptides: BUE45_RS13115_lanthipeptide

6

PSSYAIGTRCPVC

18

P+S +I + CP C

5

PNSISIASLCPSC

17

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

CG736_RS24295: PT_FPPS_like

Domain 1 (74 - 358): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 19.79 out of 25.31

Confidence: medium

Additional weak hits (10):

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.09 out of 21.55

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.08 out of 21.55

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.31 out of 32.74

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.09 out of 20.97

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.40 out of 27.78

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 19.71 out of 27.55

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 14.81 out of 21.55

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 20.69 out of 30.98

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 19.79 out of 29.65

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.24 out of 24.78

Confidence: weak

Lanthipeptides

RREFinder

Terpene

TFBS Finder

Core peptide predictions

CG736_RS24265 - Class I

Cleavage pHMM score: -3.90

RODEO score: 15

Monoisotopic mass: 1840.8 Da

Molecular weight: 1842.1 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 1860.1 Da N = 1; 1878.1 Da N = 2; 1896.2 Da N = 3

RRE predictions

: RRE type: Lanthipeptide_LanB_RRE; Location: 136 - 219; Score: 56.8

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
CatR	H2O2-responsive repressor

NZ_NNBO01000014.1 - Region 1 - azoxy-crosslink

Download region GenBank file

Download region SVG

Location: 58,587 - 86,432 nt. (total: 27,846 nt) Show pHMM detection rules used

azoxy-crosslink: (azdH and LPG_synthase_C and azdO and vlmB)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

CG736_RS27025: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

CG736_RS27030: alcohol dehydrogenase

smcogs: SMCOG1040: alcohol dehydrogenase

CG736_RS27060: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

CG736_RS27065: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

CG736_RS27085: flavin reductase domain protein FMN-binding

smcogs: SMCOG1160: flavin reductase domain protein FMN-binding

Transferases: aminotransferase

CG736_RS27070: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

Ligases: synthase

CG736_RS27100: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

CG736_RS27110: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001275.3	0.56	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002711.2	0.51	PKS	nostovalerolactone, 9-dehydronostovalerolactone	Nostoc punctiforme PCC 73102
BGC0002381.3	0.50	PKS, other (other)	tambjamine BE-18591	Streptomyces albus subsp. albus
BGC0000852.5	0.49	other (other)	ectoine	Sporosarcina pasteurii
BGC0000919.5	0.48	other (other)	phaseolotoxin	Pseudomonas syringae
BGC0001797.3	0.48	ribosomal	telomestatin	Streptomyces anulatus
BGC0000858.3	0.47	other (other)	ectoine	Methylobacter marinus
BGC0000859.3	0.47	other (other)	ectoine	Methylomicrobium alcaliphilum
BGC0000856.3	0.47	other (other)	ectoine	Methylophaga thalassica
BGC0000855.3	0.47	other (other)	ectoine	Methylomicrobium kenyense

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001275.3	0.56	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002711.2	0.51	PKS	nostovalerolactone, 9-dehydronostovalerolactone	Nostoc punctiforme PCC 73102
BGC0002381.3	0.50	PKS, other (other)	tambjamine BE-18591	Streptomyces albus subsp. albus
BGC0000852.5	0.49	other (other)	ectoine	Sporosarcina pasteurii
BGC0000919.5	0.48	other (other)	phaseolotoxin	Pseudomonas syringae
BGC0001797.3	0.48	ribosomal	telomestatin	Streptomyces anulatus
BGC0000858.3	0.47	other (other)	ectoine	Methylobacter marinus
BGC0000859.3	0.47	other (other)	ectoine	Methylomicrobium alcaliphilum
BGC0000856.3	0.47	other (other)	ectoine	Methylophaga thalassica
BGC0000855.3	0.47	other (other)	ectoine	Methylomicrobium kenyense

Similar known clusters from MIBiG 4.0

Similar subclusters

Transcription Factor Binding Sites

Binding sites found:

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 18.82 out of 21.84

Confidence: medium

Additional weak hits (5):

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.05 out of 29.38

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.29 out of 32.74

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 19.53 out of 27.55

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.72 out of 39.62

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.05 out of 30.22

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
MexT	Global virulence regulator

NZ_NNBO01000018.1 - Region 1 - NRPS,NRPS-like

Download region GenBank file

Download region SVG

Location: 5,112 - 54,259 nt. (total: 49,148 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidase

CG736_RS30705: FAD linked oxidase domain protein

smcogs: SMCOG1138: FAD linked oxidase domain protein

Transferases: aminotransferase

CG736_RS30665: aminotransferase class V

smcogs: SMCOG1139: aminotransferase class V

Hydrolases: hydrolase

CG736_RS30605: HAD-superfamily hydrolase, subfamily IA, variant

smcogs: SMCOG1115: HAD-superfamily hydrolase, subfamily IA, variant

CG736_RS30725: HAD-superfamily hydrolase, subfamily IA, variant

smcogs: SMCOG1115: HAD-superfamily hydrolase, subfamily IA, variant

CG736_RS30735: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

Lyases: lyase

CG736_RS30720: argininosuccinate lyase/adenylosuccinate lyase

smcogs: SMCOG1063: argininosuccinate lyase/adenylosuccinate lyase

Ligases: ligase

CG736_RS30690: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

CG736_RS30700: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001249.5	0.83	NRPS	dimethylcoprogen	Alternaria alternata
BGC0000342.4	0.72	NRPS	enniatin	Fusarium equiseti
BGC0001135.5	0.71	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001132.4	0.70	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002135.2	0.70	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001128.4	0.67	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.67	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002164.2	0.66	NRPS	peramine	Epichloe festucae
BGC0000307.4	0.66	NRPS	AbT1	Aureobasidium pullulans
BGC0001833.4	0.65	NRPS	icosalide A, icosalide B	Burkholderia gladioli

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001249.5	0.73	NRPS	dimethylcoprogen	Alternaria alternata
BGC0000342.4	0.69	NRPS	enniatin	Fusarium equiseti
BGC0001135.5	0.68	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001132.4	0.68	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002135.2	0.68	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001128.4	0.67	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.67	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002164.2	0.66	NRPS	peramine	Epichloe festucae
BGC0000307.4	0.66	NRPS	AbT1	Aureobasidium pullulans
BGC0001833.4	0.66	NRPS	icosalide A, icosalide B	Burkholderia gladioli

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 21.93 out of 26.33

Confidence: medium

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 21.93 out of 26.33

Confidence: medium

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 26.07 out of 30.12

Confidence: medium

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 18.33 out of 19.52

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.47 out of 23.79

Confidence: medium

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 24.85 out of 30.12

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.76 out of 23.05

Confidence: medium

Additional weak hits (6):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 17.99 out of 26.33

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.14 out of 21.55

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.77 out of 21.55

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.18 out of 29.65

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.23 out of 29.65

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.03 out of 35.63

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 5111 - 54259:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (X - Ala)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 5111 - 46887:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (X - Ala)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 7845 - 54259:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (X - Ala)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

CG736_RS30690: X

AMP-binding (13..412): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: MNYNTDSAVSDHDAIVGGEFNLYGPTECTVAVTF
Nearest Stachelhaus code(s):: Lys or Arg DVEDIGSVAK (47% 8Å match)
Stachelhaus code match:: 70% (weak)

CG736_RS30700: X - Ala

Search NORINE for peptide: strict or relaxed

AMP-binding (30..428): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FDRSWDVFALEGYMFTGGEVNGYSPLECTILSVC
Nearest Stachelhaus code(s):: Ser DFDTFGGILK (44% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (1095..1503): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: ser (Ser)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: HAVAFDVSIWQTQILVHGEVNVYGPAECSDDVSI
Nearest Stachelhaus code(s):: ohDab DVWQMIGDDK (53% 8Å match)
Stachelhaus code match:: 70% (weak)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
ANR	Anaerobic transcriptional regulator
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
AfsR	Pleiotropic regulatory for antibiotic production
IolR	Regulator of the inositol catabolism
OsdR	Development and stress management regulator

NZ_NNBO01000002.1 - Region 1 - NRPS,PKS-like,T1PKS

Download region GenBank file

Download region SVG

Location: 104,687 - 202,784 nt. (total: 98,098 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
PKS-like: (t2pks2 or ksIII)
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

CG736_RS06105: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

CG736_RS06205: Dioxygenase TauD/TfdA

smcogs: SMCOG1046: Dioxygenase TauD/TfdA

CG736_RS06285: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

CG736_RS38855: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: glycosyltransferase

CG736_RS06145: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

Hydrolases: esterase

CG736_RS06195: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: ligase synthase

CG736_RS06165: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

CG736_RS06170: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

CG736_RS06180: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

CG736_RS06185: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

CG736_RS06190: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

CG736_RS37480: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

CG736_RS37485: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	1.13	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	1.13	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002286.3	1.11	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001244.3	1.10	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001833.4	1.09	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0000342.4	1.08	NRPS	enniatin	Fusarium equiseti
BGC0001250.4	1.08	NRPS	epichloenin A	Epichloe festucae
BGC0001251.4	1.08	NRPS	epichloenin A	Epichloe festucae
BGC0001641.4	1.06	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002135.2	1.04	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.74	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.74	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001276.3	0.74	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.73	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0002286.3	0.70	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.69	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0000342.4	0.69	NRPS	enniatin	Fusarium equiseti
BGC0001250.4	0.69	NRPS	epichloenin A	Epichloe festucae
BGC0001251.4	0.69	NRPS	epichloenin A	Epichloe festucae
BGC0001641.4	0.68	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 21.64 out of 26.33

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.67 out of 19.26

Confidence: medium

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 22.77 out of 27.04

Confidence: medium

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 17.11 out of 19.52

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.45 out of 23.79

Confidence: medium

Additional weak hits (10):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.59 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 12.67 out of 26.06

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.35 out of 29.65

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.64 out of 32.93

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 19.60 out of 29.65

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 16.24 out of 29.25

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 17.33 out of 24.78

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.66 out of 35.63

Confidence: weak

CsoR

Description: Copper-responsive repressor
Species: Streptomyces lividans
Score: 22.48 out of 32.38

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.44 out of 29.65

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 104686 - 202784:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk - Me-ohmal - pk - pk - ccmal) + (pk - pk - ccmal) + (Cys) + (Val) + (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

CG736_RS37480: pk - mmal - pk - pk - mal

PKS_AT (506..801): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 209.9
Methylmalonyl-CoA: 208.8
Methoxymalonyl-CoA: 169.1
2-Methylbutyryl-CoA: 145.0
Propionyl-CoA: 136.8
trans-1,2-CPDA: 123.4
CHC-CoA: 119.1
Malonyl-CoA: 107.1
Isobutyryl-CoA: 87.1
Benzoyl-CoA: 66.0
Acetyl-CoA: 54.6
fatty_acid: 41.7
3-Methylbutyryl-CoA: 34.0
inactive: 31.0

PKS_AT (1508..1788): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 87.5%; Malonyl-CoA: 79.2%; Ethylmalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 196.4
Ethylmalonyl-CoA: 194.3
Methoxymalonyl-CoA: 158.3
2-Methylbutyryl-CoA: 127.3
trans-1,2-CPDA: 124.0
Malonyl-CoA: 104.2
Propionyl-CoA: 98.0
CHC-CoA: 90.1
Isobutyryl-CoA: 88.4
Benzoyl-CoA: 61.6
Acetyl-CoA: 48.4
inactive: 33.7
fatty_acid: 28.6
3-Methylbutyryl-CoA: 12.2

PKS_AT (2923..3197): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Malonyl-CoA: 87.5%; Ethylmalonyl-CoA: 87.5%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 226.5
Methylmalonyl-CoA: 214.2
Methoxymalonyl-CoA: 177.6
2-Methylbutyryl-CoA: 149.9
trans-1,2-CPDA: 127.0
Propionyl-CoA: 117.3
CHC-CoA: 112.5
Malonyl-CoA: 105.7
Isobutyryl-CoA: 89.8
Benzoyl-CoA: 77.0
Acetyl-CoA: 61.1
3-Methylbutyryl-CoA: 52.2
inactive: 32.2
fatty_acid: 30.5

PKS_AT (4820..5098): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Malonyl-CoA: 83.3%; Ethylmalonyl-CoA: 83.3%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 215.0
Methylmalonyl-CoA: 208.3
Methoxymalonyl-CoA: 173.8
2-Methylbutyryl-CoA: 144.8
trans-1,2-CPDA: 118.5
Malonyl-CoA: 115.3
Propionyl-CoA: 114.7
CHC-CoA: 109.8
Isobutyryl-CoA: 85.0
Benzoyl-CoA: 74.9
Acetyl-CoA: 59.2
3-Methylbutyryl-CoA: 44.3
inactive: 41.2
fatty_acid: 35.6

PKS_AT (6443..6707): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 75.0%; Methylmalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 183.2
inactive: 92.9
Methoxymalonyl-CoA: 76.6
Methylmalonyl-CoA: 74.6
Isobutyryl-CoA: 41.3
Acetyl-CoA: 32.9
Benzoyl-CoA: 27.5
Propionyl-CoA: 23.1
CHC-CoA: 17.9
fatty_acid: 9.6
trans-1,2-CPDA: 7.4
3-Methylbutyryl-CoA: 3.1
Ethylmalonyl-CoA: -1.4
2-Methylbutyryl-CoA: -3.7

CG736_RS37485: pk - pk - mal

PKS_AT (550..844): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Malonyl-CoA: 83.3%; Ethylmalonyl-CoA: 83.3%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 218.8
Methylmalonyl-CoA: 214.3
Methoxymalonyl-CoA: 186.2
2-Methylbutyryl-CoA: 147.8
trans-1,2-CPDA: 125.6
Malonyl-CoA: 120.7
Propionyl-CoA: 117.2
CHC-CoA: 113.4
Isobutyryl-CoA: 89.3
Benzoyl-CoA: 81.2
Acetyl-CoA: 55.9
fatty_acid: 42.4
inactive: 35.7
3-Methylbutyryl-CoA: 35.3

PKS_AT (2490..2779): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Malonyl-CoA: 83.3%; Ethylmalonyl-CoA: 83.3%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 220.6
Methylmalonyl-CoA: 208.4
Methoxymalonyl-CoA: 174.4
2-Methylbutyryl-CoA: 157.6
trans-1,2-CPDA: 128.5
CHC-CoA: 116.8
Propionyl-CoA: 116.5
Malonyl-CoA: 113.8
Isobutyryl-CoA: 77.4
Benzoyl-CoA: 73.3
Acetyl-CoA: 46.7
3-Methylbutyryl-CoA: 38.2
fatty_acid: 32.3
inactive: 30.5

PKS_AT (4404..4668): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 75.0%; Methylmalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 183.2
inactive: 92.9
Methoxymalonyl-CoA: 76.6
Methylmalonyl-CoA: 74.6
Isobutyryl-CoA: 41.3
Acetyl-CoA: 32.9
Benzoyl-CoA: 27.5
Propionyl-CoA: 23.1
CHC-CoA: 17.9
fatty_acid: 9.6
trans-1,2-CPDA: 7.4
3-Methylbutyryl-CoA: 3.1
Ethylmalonyl-CoA: -1.4
2-Methylbutyryl-CoA: -3.7

CG736_RS06165: Cys

Search NORINE for peptide: strict or relaxed

AMP-binding (459..855): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LPGHTDLATWEGNQVVSGAVGLYAVTEGGIYVTG
Nearest Stachelhaus code(s):: Cys DLWNLSLIWK (35% 8Å match)
Stachelhaus code match:: 80% (moderate)

CG736_RS06180: Val

Search NORINE for peptide: strict or relaxed

AMP-binding (555..954): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LDESFDASTLEVWLLTGGGTNGYGPTENTTFTTV
Nearest Stachelhaus code(s):: Val DALWLGGTFK (79% 8Å match); bOH-Val DALWLGGTFK (71% 8Å match)
Stachelhaus code match:: 100% (strong)

CG736_RS06185: mal

PKS_AT (521..809): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 66.7%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 104.2
Methoxymalonyl-CoA: 59.7
Methylmalonyl-CoA: 45.2
inactive: 38.9
Propionyl-CoA: 23.3
CHC-CoA: 18.6
Ethylmalonyl-CoA: 14.5
fatty_acid: 12.7
Isobutyryl-CoA: 12.6
2-Methylbutyryl-CoA: 12.0
trans-1,2-CPDA: 8.6
Acetyl-CoA: 5.2
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

CG736_RS06190: Ser

Search NORINE for peptide: strict or relaxed

AMP-binding (37..437): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: RSMTFDVSIWELHFLSGGEYNMYGPAEATIDVTA
Nearest Stachelhaus code(s):: Ser DVWHLSLIDK (74% 8Å match)
Stachelhaus code match:: 80% (moderate)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
ANR	Anaerobic transcriptional regulator
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
CRP	Cyclic AMP receptor protein
DasR	N-acetylglucosamine dependent repressor
IolR	Regulator of the inositol catabolism

NZ_NNBO01000002.1 - Region 2 - lanthipeptide-class-iii

Download region GenBank file

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Location: 234,817 - 262,150 nt. (total: 27,334 nt) Show pHMM detection rules used

lanthipeptide-class-iii: (micKC and not PKS_KS)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase

CG736_RS06480: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

Transferases: acetyltransferase

CG736_RS06475: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

Hydrolases: protease

CG736_RS06520: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000528.5	0.54	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0002316.2	0.51	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0000503.5	0.49	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0002346.3	0.48	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0000519.4	0.48	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0000518.3	0.48	ribosomal	informatipeptin	Streptomyces viridochromogenes DSM 40736
BGC0000500.5	0.45	ribosomal	carnolysin A1, carnolysin A2	Carnobacterium maltaromaticum
BGC0002701.2	0.44	ribosomal	paenithipeptin	Paenibacillus thiaminolyticus
BGC0000874.5	0.40	other (other)	blasticidin S	Streptomyces griseochromogenes
BGC0000903.3	0.40	other (other)	fluorometabolite	Streptomyces cattleya

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000528.5	0.54	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0002316.2	0.51	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0000503.5	0.49	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0002346.3	0.48	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0000519.4	0.48	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0000518.3	0.48	ribosomal	informatipeptin	Streptomyces viridochromogenes DSM 40736
BGC0000500.5	0.45	ribosomal	carnolysin A1, carnolysin A2	Carnobacterium maltaromaticum
BGC0002701.2	0.44	ribosomal	paenithipeptin	Paenibacillus thiaminolyticus
BGC0000874.5	0.40	other (other)	blasticidin S	Streptomyces griseochromogenes
BGC0000903.3	0.40	other (other)	fluorometabolite	Streptomyces cattleya

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for CG736_RS06510

CG736_RS35985

Leader:

MSINPTPEDNAAEVEAVLSLQEAPTAEAQDDVE

Core:

AHISSVSVDHCANEGTKGRI

Core with dehydrations:

AHIDhaDhaVDhaVDHCysANEGDhbKGRI

antiSMASH-DB 4.0 matches for CG736_RS35985

100.0%

NZ_NNBO01000002.1:

Lanthipeptides: CG736_RS35985_lanthipeptide

1

AHISSVSVDHCANEGTKGRI

20

AHISSVSVDHCANEGTKGRI

1

AHISSVSVDHCANEGTKGRI

20

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 19.28 out of 23.05

Confidence: medium

Additional weak hits (10):

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 18.77 out of 33.73

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 20.59 out of 27.55

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.29 out of 21.55

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.01 out of 29.38

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.59 out of 22.40

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.59 out of 22.40

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.94 out of 22.40

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 19.42 out of 29.65

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.13 out of 24.78

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.52 out of 24.78

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

CG736_RS35985 - Class III

Cleavage pHMM score: -3.20

RODEO score: 0

Monoisotopic mass: 2008.0 Da

Molecular weight: 2009.2 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2027.2 Da N = 1; 2045.2 Da N = 2; 2063.3 Da N = 3

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production

NZ_NNBO01000002.1 - Region 3 - lanthipeptide-class-iv

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Location: 425,758 - 448,280 nt. (total: 22,523 nt) Show pHMM detection rules used

lanthipeptide-class-iv: (cds(LANC_like and Pkinase) and not micKC)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: kinase

CG736_RS07410: glycerol kinase

smcogs: SMCOG1210: glycerol kinase

Hydrolases: hydrolase

CG736_RS07385: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Translocases: transport

CG736_RS07395: MMPL domain-containing transport protein

smcogs: SMCOG1035: MMPL domain-containing transport protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002337.2	0.83	ribosomal	class IV lanthipeptide, SflA	Streptomyces sp. NRRL S-1022
BGC0001164.3	0.55	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000513.3	0.46	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0002150.2	0.43	ribosomal	triculamin	Streptomyces triculaminicus
BGC0002151.2	0.43	ribosomal	alboverticillin	Streptomyces griseocarneus
BGC0000558.5	0.42	ribosomal	sublancin 168	Bacillus subtilis subsp. subtilis str. 168
BGC0001579.4	0.42	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0001660.3	0.41	ribosomal	mathermycin	Marinactinospora thermotolerans
BGC0001210.5	0.40	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0001639.3	0.40	ribosomal	klebsidin	Klebsiella pneumoniae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002337.2	0.83	ribosomal	class IV lanthipeptide, SflA	Streptomyces sp. NRRL S-1022
BGC0001164.3	0.55	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000513.3	0.46	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0002150.2	0.43	ribosomal	triculamin	Streptomyces triculaminicus
BGC0002151.2	0.43	ribosomal	alboverticillin	Streptomyces griseocarneus
BGC0000558.5	0.42	ribosomal	sublancin 168	Bacillus subtilis subsp. subtilis str. 168
BGC0001579.4	0.42	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0001660.3	0.41	ribosomal	mathermycin	Marinactinospora thermotolerans
BGC0001210.5	0.40	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0001639.3	0.40	ribosomal	klebsidin	Klebsiella pneumoniae

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptides - No core peptides found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (11):

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 15.62 out of 31.73

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 15.52 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.47 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.61 out of 28.14

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 14.01 out of 21.55

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 15.76 out of 31.73

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 19.57 out of 26.33

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.58 out of 27.78

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 20.59 out of 30.97

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 20.02 out of 27.18

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.11 out of 26.85

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

No core peptides found.

TFBS definitions

Regulator	Description

NZ_NNBO01000020.1 - Region 1 - RiPP-like

Download region GenBank file

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Location: 71,069 - 82,347 nt. (total: 11,279 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

CG736_RS32305: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002788.2	0.23	PKS	14-hydroxyisochainin	Streptomyces peucetius subsp. caesius ATCC 27952
BGC0000241.5	0.19	PKS, saccharide	lomaiviticin A, lomaiviticin C, lomaiviticin D, lomaiviticin E	Salinispora tropica CNB-440
BGC0001693.3	0.19	PKS	nenestatin	Micromonospora echinospora

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002788.2	0.23	PKS	14-hydroxyisochainin	Streptomyces peucetius subsp. caesius ATCC 27952
BGC0000241.5	0.19	PKS, saccharide	lomaiviticin A, lomaiviticin C, lomaiviticin D, lomaiviticin E	Salinispora tropica CNB-440
BGC0001693.3	0.19	PKS	nenestatin	Micromonospora echinospora

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.25 out of 20.47

Confidence: medium

Additional weak hits (1):

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.03 out of 32.93

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
VqsM	Regulator of quorum-sensing signalling systems

NZ_NNBO01000021.1 - Region 1 - NI-siderophore

Download region GenBank file

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Location: 46,298 - 79,742 nt. (total: 33,445 nt) Show pHMM detection rules used

NI-siderophore: IucA_IucC

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxygenase

CG736_RS32905: lysine/ornithine N-monooxygenase

smcogs: SMCOG1080: lysine/ornithine N-monooxygenase

Translocases: transporter

CG736_RS32955: sugar-binding lipoprotein

smcogs: SMCOG1143: sugar-binding lipoprotein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002692.2	0.64	other (other)	speibonoxamine, desoxy-desferrioxamine D1, desferrioxamine D1, desferrioxamine B, didesoxy-desferrioxamine D1, desoxy-desferrioxamine B, didesoxy-desferrioxamine B	Kribbella speibonae
BGC0001572.3	0.64	other (other)	desferrioxamine E	Pantoea agglomerans
BGC0002469.2	0.63	other (other)	woodybactin A, woodybactin B, woodybactin C, woodybactin D	Shewanella woodyi ATCC 51908
BGC0001713.5	0.59	other (other)	ochrobactin	Ochrobactrum anthropi
BGC0000941.3	0.57	other (other)	desferrioxamin B	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000295.5	0.57	NRPS	acinetoferrin	Acinetobacter haemolyticus
BGC0002305.2	0.57	other (other)	legonoxamine A, desferrioxamine B, legonoxamine B	Streptomyces sp.
BGC0001531.5	0.56	other (other)	bisucaberin B	Tenacibaculum mesophilum
BGC0002464.3	0.54	other (other)	alcaligin	Bordetella pertussis Tohama I
BGC0001499.5	0.54	other (other)	aerobactin	Pantoea ananatis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002692.2	0.64	other (other)	speibonoxamine, desoxy-desferrioxamine D1, desferrioxamine D1, desferrioxamine B, didesoxy-desferrioxamine D1, desoxy-desferrioxamine B, didesoxy-desferrioxamine B	Kribbella speibonae
BGC0001572.3	0.64	other (other)	desferrioxamine E	Pantoea agglomerans
BGC0002469.2	0.63	other (other)	woodybactin A, woodybactin B, woodybactin C, woodybactin D	Shewanella woodyi ATCC 51908
BGC0001713.5	0.59	other (other)	ochrobactin	Ochrobactrum anthropi
BGC0000941.3	0.57	other (other)	desferrioxamin B	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000295.5	0.57	NRPS	acinetoferrin	Acinetobacter haemolyticus
BGC0002305.2	0.57	other (other)	legonoxamine A, desferrioxamine B, legonoxamine B	Streptomyces sp.
BGC0001531.5	0.56	other (other)	bisucaberin B	Tenacibaculum mesophilum
BGC0002464.3	0.54	other (other)	alcaligin	Bordetella pertussis Tohama I
BGC0001499.5	0.54	other (other)	aerobactin	Pantoea ananatis

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.80 out of 20.47

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 20.93 out of 24.78

Confidence: medium

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 25.17 out of 29.65

Confidence: medium

Additional weak hits (18):

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 20.45 out of 32.74

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 18.38 out of 21.84

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 19.77 out of 25.88

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.31 out of 26.06

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.20 out of 35.63

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.62 out of 28.19

Confidence: weak

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 16.79 out of 19.52

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.59 out of 27.78

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 21.99 out of 36.53

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 20.95 out of 32.74

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 23.09 out of 29.65

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 17.65 out of 24.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.24 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.35 out of 26.06

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 20.71 out of 27.18

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 20.71 out of 27.18

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 20.08 out of 27.18

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 13.82 out of 18.57

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
LexA variant 1	Repressor of DNA damage response
LexA variant 2	Repressor of DNA damage response
VqsM	Regulator of quorum-sensing signalling systems

NZ_NNBO01000022.1 - Region 1 - NI-siderophore,NRPS,t3nrps-iterative

Download region GenBank file

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Location: 1 - 72,026 nt. (total: 72,026 nt) Show pHMM detection rules used

Region on contig edge.

t3nrps-iterative: (AMP-binding and PP-binding and (DUF98 or Orn_Arg_deC_N or Orn_DAP_Arg_deC) and not ATP-grasp)
NI-siderophore: IucA_IucC
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxygenase

CG736_RS33385: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: acetyltransferase

CG736_RS33425: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

Hydrolases: dehalogenase hydrolase

CG736_RS33340: haloalkane dehalogenase

smcogs: SMCOG1262: haloalkane dehalogenase

CG736_RS33465: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Lyases: decarboxylase

CG736_RS33325: diaminopimelate decarboxylase

smcogs: SMCOG1264: diaminopimelate decarboxylase

CG736_RS33415: putative carboxymuconolactone decarboxylase

smcogs: SMCOG1298: putative carboxymuconolactone decarboxylase

Ligases: ligase

CG736_RS33375: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001833.4	0.87	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.86	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002135.2	0.86	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001128.4	0.84	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.83	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.82	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825.4	0.82	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001135.5	0.81	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0000307.4	0.75	NRPS	AbT1	Aureobasidium pullulans
BGC0001261.3	0.74	NRPS	AM-toxin	Alternaria alternata

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001833.4	0.74	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.74	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002135.2	0.74	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001128.4	0.73	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.73	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.73	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825.4	0.73	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001135.5	0.72	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0000307.4	0.70	NRPS	AbT1	Aureobasidium pullulans
BGC0001261.3	0.69	NRPS	AM-toxin	Alternaria alternata

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.00 out of 20.47

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.73 out of 23.79

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.25 out of 20.47

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.33 out of 23.79

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.15 out of 23.79

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.15 out of 23.79

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.02 out of 23.79

Confidence: medium

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 23.00 out of 29.72

Confidence: medium

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 24.85 out of 30.12

Confidence: medium

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 24.85 out of 30.12

Confidence: medium

Additional weak hits (10):

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.78 out of 39.62

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.24 out of 39.62

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 15.56 out of 21.84

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 12.98 out of 28.14

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.44 out of 27.78

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 12.96 out of 28.14

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.09 out of 26.06

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 20.93 out of 32.74

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.53 out of 27.78

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.59 out of 22.40

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 0 - 72026:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - Thr - Tyr - Pro - Phe - NMe-Tyr - Val)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

CG736_RS33375: X - Thr - Tyr - Pro - Phe - Tyr - Val

Search NORINE for peptide: strict or relaxed

AMP-binding (467..871): X

nrpys: Asp, Asn, Glu, Gln, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LDLTFDASWNQAEAAAGGELNFYGPSECTVNSVV
Nearest Stachelhaus code(s):: Lys DADDAGTVDK (56% 8Å match)
Stachelhaus code match:: 60% (weak)

AMP-binding (1526..1929): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: LTTHFDFSVWEGNQVFGGEVNMYGITETTVHVTY
Nearest Stachelhaus code(s):: Thr DFWNVGMVHK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2589..2989): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: LDQTFDASCDQAGGGLGGEVNYYGPSECTVDSVF
Nearest Stachelhaus code(s):: Lys DADDGGFVDK (56% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (3644..4029): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: LWHTFDVSVQESFAAQAGEHNHYGPAETHVITAY
Nearest Stachelhaus code(s):: Pro DVQFAAHVVK (91% 8Å match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (4681..5049): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: phe (Phe)

Stachelhaus prediction details:

Stachelhaus sequence:: LAQAFDASFWDSTLVVAGEINAYGPTETTVCATM
Nearest Stachelhaus code(s):: Phe DAWTVAAVCK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (5704..6072): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: LAQAFDASVSETTVVVAGEINAYGPTETTVCATM
Nearest Stachelhaus code(s):: Tyr DASTVAAVCK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (7138..7519): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LTPSFDLSTYEVWLWTGGDVNGYGPTEATTLATC
Nearest Stachelhaus code(s):: Val DAYWWGGTFK (82% 8Å match)
Stachelhaus code match:: 80% (moderate)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism
NtrC	Nitrogen regulatory protein C
OsdR	Development and stress management regulator
VqsM	Regulator of quorum-sensing signalling systems

NZ_NNBO01000003.1 - Region 1 - lanthipeptide-class-iv

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Download region SVG

Location: 283,428 - 306,241 nt. (total: 22,814 nt) Show pHMM detection rules used

lanthipeptide-class-iv: (cds(LANC_like and Pkinase) and not micKC)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

CG736_RS10120: alcohol dehydrogenase

smcogs: SMCOG1040: alcohol dehydrogenase

Isomerases: mutase

CG736_RS10150: phosphoglycerate mutase

smcogs: SMCOG1101: phosphoglycerate mutase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002337.2	0.57	ribosomal	class IV lanthipeptide, SflA	Streptomyces sp. NRRL S-1022
BGC0001226.4	0.50	ribosomal	streptocollin	Streptomyces collinus Tu 365
BGC0000563.4	0.50	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712
BGC0002700.2	0.49	ribosomal	bacinapeptin	Bacillus nakamurai
BGC0002111.3	0.46	ribosomal	andalusicin A, andalusicin B	Bacillus thuringiensis serovar andalousiensis NRRL B-23139
BGC0002701.2	0.43	ribosomal	paenithipeptin	Paenibacillus thiaminolyticus
BGC0001948.3	0.43	other (other)	naseseazine C, C3-aryl pyrroloindolines	Streptomyces sp.
BGC0000518.3	0.42	ribosomal	informatipeptin	Streptomyces viridochromogenes DSM 40736
BGC0002227.3	0.39	NRPS	AKML B, AKML D, AKML A, AKML C	Aspergillus luchuensis IFO 4308
BGC0002496.3	0.39	NRPS	serratiochelin A, serratiochelin C, serratiochelin B	Serratia plymuthica

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002337.2	0.57	ribosomal	class IV lanthipeptide, SflA	Streptomyces sp. NRRL S-1022
BGC0001226.4	0.50	ribosomal	streptocollin	Streptomyces collinus Tu 365
BGC0000563.4	0.50	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712
BGC0002700.2	0.49	ribosomal	bacinapeptin	Bacillus nakamurai
BGC0002111.3	0.46	ribosomal	andalusicin A, andalusicin B	Bacillus thuringiensis serovar andalousiensis NRRL B-23139
BGC0002701.2	0.43	ribosomal	paenithipeptin	Paenibacillus thiaminolyticus
BGC0001948.3	0.43	other (other)	naseseazine C, C3-aryl pyrroloindolines	Streptomyces sp.
BGC0000518.3	0.42	ribosomal	informatipeptin	Streptomyces viridochromogenes DSM 40736
BGC0002227.3	0.39	NRPS	AKML B, AKML D, AKML A, AKML C	Aspergillus luchuensis IFO 4308
BGC0002496.3	0.39	NRPS	serratiochelin A, serratiochelin C, serratiochelin B	Serratia plymuthica

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptides - No core peptides found.

Transcription Factor Binding Sites

Binding sites found:

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 19.78 out of 20.97

Confidence: strong

Additional weak hits (7):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 13.92 out of 27.78

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 16.73 out of 30.25

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.27 out of 20.97

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.89 out of 26.06

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 15.86 out of 28.19

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 19.49 out of 27.04

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.92 out of 25.75

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

No core peptides found.

TFBS definitions

Regulator	Description
CcpA	Catabolite control protein A

NZ_NNBO01000003.1 - Region 2 - terpene-precursor

Download region GenBank file

Download region SVG

Location: 505,073 - 526,179 nt. (total: 21,107 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

CG736_RS11065: FAD dependent oxidoreductase

smcogs: SMCOG1103: FAD dependent oxidoreductase

CG736_RS11070: pyridine nucleotide-disulfide oxidoreductase

smcogs: SMCOG1175: pyridine nucleotide-disulfide oxidoreductase

CG736_RS11105: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

Transferases: phosphorylase

CG736_RS11080: thiamine-phosphate pyrophosphorylase

smcogs: SMCOG1280: thiamine-phosphate pyrophosphorylase

Ligases: synthetase

CG736_RS11090: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000647.3	0.45	terpene	carotenoid	Rhodobacter sphaeroides
BGC0002345.2	0.44	other (other)	hydrogen cyanide	Pseudomonas fluorescens
BGC0000677.5	0.43	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0000664.5	0.41	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001456.3	0.41	terpene	isorenieratene	Streptomyces argillaceus
BGC0000648.3	0.41	terpene	carotenoid	Myxococcus xanthus
BGC0000649.3	0.40	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001227.3	0.40	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000633.3	0.40	terpene	carotenoid	Streptomyces avermitilis
BGC0000678.5	0.40	terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000647.3	0.45	terpene	carotenoid	Rhodobacter sphaeroides
BGC0002345.2	0.44	other (other)	hydrogen cyanide	Pseudomonas fluorescens
BGC0000677.5	0.43	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0000664.5	0.41	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001456.3	0.41	terpene	isorenieratene	Streptomyces argillaceus
BGC0000648.3	0.41	terpene	carotenoid	Myxococcus xanthus
BGC0000649.3	0.40	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001227.3	0.40	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000633.3	0.40	terpene	carotenoid	Streptomyces avermitilis
BGC0000678.5	0.40	terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

CG736_RS11090: PT_FPPS_like

Domain 1 (48 - 367): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.35 out of 23.79

Confidence: medium

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 26.90 out of 30.12

Confidence: medium

Additional weak hits (7):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.18 out of 26.33

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.74 out of 27.78

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 19.41 out of 26.33

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.22 out of 39.62

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.58 out of 30.22

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 20.26 out of 27.18

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 20.83 out of 36.53

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism
OsdR	Development and stress management regulator

NZ_NNBO01000004.1 - Region 1 - NRPS,NRPS-like,PKS-like,T1PKS,T2PKS,aminopolycarboxylic-acid,lanthipeptide-class-iii,t3nrps-iterative

Download region GenBank file

Download region SVG

Location: 71,462 - 306,145 nt. (total: 234,684 nt) Show pHMM detection rules used

T2PKS: (t2ks and t2clf)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
PKS-like: (t2pks2 or ksIII)
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
lanthipeptide-class-iii: (micKC and not PKS_KS)
t3nrps-iterative: (AMP-binding and PP-binding and (DUF98 or Orn_Arg_deC_N or Orn_DAP_Arg_deC) and not ATP-grasp)
aminopolycarboxylic-acid: (AesA and AesB and AesC)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase reductase

CG736_RS11440: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

CG736_RS11515: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

CG736_RS11605: flavin reductase domain protein FMN-binding

smcogs: SMCOG1160: flavin reductase domain protein FMN-binding

CG736_RS11615: acyl-CoA dehydrogenase type 2

smcogs: SMCOG1191: acyl-CoA dehydrogenase type 2

CG736_RS11620: oxidoreductase

smcogs: SMCOG1083: oxidoreductase

CG736_RS11640: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

CG736_RS11645: 3-hydroxybutyryl-CoA dehydrogenase

smcogs: SMCOG1095: 3-hydroxybutyryl-CoA dehydrogenase

CG736_RS11655: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

CG736_RS11680: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

CG736_RS11730: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

CG736_RS11750: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

CG736_RS11805: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

CG736_RS11815: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

CG736_RS11880: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

CG736_RS11945: dehydrogenase

smcogs: SMCOG1072: dehydrogenase

CG736_RS11990: alcohol dehydrogenase

smcogs: SMCOG1040: alcohol dehydrogenase

CG736_RS12115: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

CG736_RS12130: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

CG736_RS12145: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

CG736_RS12215: alcohol dehydrogenase

smcogs: SMCOG1040: alcohol dehydrogenase

CG736_RS12225: alkyl hydroperoxide reductase/ Thiol specific

smcogs: SMCOG1196: alkyl hydroperoxide reductase/ Thiol specific

CG736_RS12235: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

CG736_RS12240: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

CG736_RS12245: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

CG736_RS12260: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: PPTase acylase aminotransferase methyltransferase

CG736_RS11590: methyltransferase

smcogs: SMCOG1248: methyltransferase

CG736_RS11660: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

CG736_RS11670: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

CG736_RS11755: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1012: 4'-phosphopantetheinyl transferase

CG736_RS11770: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

CG736_RS11820: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

CG736_RS12120: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

Hydrolases: deaminase dehalogenase esterase hydrolase protease

CG736_RS11520: haloalkane dehalogenase

smcogs: SMCOG1262: haloalkane dehalogenase

CG736_RS11540: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

CG736_RS11600: metallo-beta-lactamase family protein

smcogs: SMCOG1170: metallo-beta-lactamase family protein

CG736_RS11905: thioesterase

smcogs: SMCOG1004: thioesterase

CG736_RS11925: metallo-beta-lactamase family protein

smcogs: SMCOG1170: metallo-beta-lactamase family protein

CG736_RS12015: haloalkane dehalogenase

smcogs: SMCOG1262: haloalkane dehalogenase

CG736_RS12180: ornithine cyclodeaminase

smcogs: SMCOG1158: ornithine cyclodeaminase

Lyases: cyclase decarboxylase dehydratase

CG736_RS11650: cyclase/dehydrase

smcogs: SMCOG1128: cyclase/dehydrase

CG736_RS11740: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

CG736_RS11810: cyclase/dehydrase

smcogs: SMCOG1128: cyclase/dehydrase

CG736_RS12095: diaminopimelate decarboxylase

smcogs: SMCOG1264: diaminopimelate decarboxylase

CG736_RS12175: diaminopimelate decarboxylase

smcogs: SMCOG1264: diaminopimelate decarboxylase

Ligases: ligase synthase

CG736_RS11595: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

CG736_RS11625: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

CG736_RS11630: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

CG736_RS11665: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

CG736_RS11675: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

CG736_RS11795: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

CG736_RS11830: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

CG736_RS11835: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

CG736_RS11840: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

CG736_RS11885: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

CG736_RS11890: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

CG736_RS11895: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

CG736_RS11900: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

CG736_RS12170: cysteine synthase

smcogs: SMCOG1081: cysteine synthase

CG736_RS12250: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Translocases: transporter

CG736_RS12065: sugar-binding lipoprotein

smcogs: SMCOG1143: sugar-binding lipoprotein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	3.80	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	3.80	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	3.73	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001276.3	3.38	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0002192.3	3.28	PKS	FR901512	fungal sp. No.14919
BGC0002191.2	3.27	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001254.3	3.21	PKS	ACT-Toxin II	Alternaria alternata
BGC0001268.4	3.16	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0000231.5	3.00	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0001164.3	2.98	PKS	1-heptadecene	Cyanothece sp. PCC 7822

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001587.5	0.83	other (other)	ethylenediaminesuccinic acid hydroxyarginine (EDHA)	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0002568.2	0.83	other (other)	Ethylenediaminesuccinic acid hydroxyarginine	Streptomyces scabiei 87.22
BGC0000231.5	0.78	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0001833.4	0.78	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.78	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001043.4	0.77	NRPS, PKS	SGR PTMs, SGR PTM Compound b, SGR PTM Compound c, SGR PTM Compound d	Streptomyces griseus subsp. griseus NBRC 13350
BGC0002365.2	0.77	PKS, other (other)	10-epi-HSAF, 10-epi-3-deOH-HSAF, 10-epi-maltophilin, 10-epi-xanthobaccin C, 10-epi-hydroxymaltophilin, 10-epi-FI-2	Streptomyces sp.
BGC0001273.3	0.77	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.77	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum

Similar known clusters from MIBiG 4.0

Similar subclusters

Lanthipeptide predictions

Lanthipeptides - No core peptides found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 19.35 out of 20.47

Confidence: strong

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.90 out of 23.79

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.00 out of 23.79

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 20.44 out of 27.78

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.39 out of 19.26

Confidence: strong

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 24.85 out of 30.12

Confidence: medium

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 25.37 out of 30.12

Confidence: medium

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 29.02 out of 30.11

Confidence: strong

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 28.15 out of 30.11

Confidence: strong

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.46 out of 23.05

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.46 out of 23.05

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 27.84 out of 36.53

Confidence: medium

Additional weak hits (46):

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.27 out of 30.25

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.40 out of 21.84

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 23.78 out of 30.22

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.41 out of 25.75

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.33 out of 25.75

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.96 out of 26.33

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 19.95 out of 27.55

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.12 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.80 out of 26.06

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 14.10 out of 21.55

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.92 out of 26.33

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 18.77 out of 35.63

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 18.89 out of 35.63

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 17.32 out of 24.78

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.85 out of 20.01

Confidence: weak

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 17.99 out of 25.31

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.29 out of 18.57

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.84 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.15 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.36 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 12.52 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.22 out of 26.06

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 19.09 out of 30.25

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 12.71 out of 26.06

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.73 out of 18.57

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.15 out of 29.38

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.24 out of 32.74

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.90 out of 27.78

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 19.95 out of 25.88

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.73 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 12.62 out of 26.06

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.63 out of 28.19

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 19.79 out of 30.98

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.33 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.94 out of 28.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.14 out of 28.19

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.12 out of 20.01

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 19.59 out of 33.73

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.53 out of 30.25

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 20.87 out of 27.04

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 17.68 out of 25.88

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.79 out of 26.85

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.09 out of 28.19

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 25.90 out of 36.53

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 22.08 out of 36.53

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.76 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

Lanthipeptides

NRPS/PKS products

NRPS/PKS substrates

Type II PKS

TFBS Finder

Core peptide predictions

No core peptides found.

Predicted core structure(s)

For candidate cluster 1, location 71461 - 217805:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-redmal) + (mal) + (Acetyl-CoA - Me-redmal) + (ohmal) + (Ser) + (ccmal) + (Gly - Tyr)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 71461 - 143938:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal) + (Me-redmal)
Non-colinear ordering:: CG736_RS11665 + CG736_RS11660

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 94635 - 143661:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal) + (Me-redmal)
Non-colinear ordering:: CG736_RS11665 + CG736_RS11660

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 105002 - 146061:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal) + (Me-redmal)
Non-colinear ordering:: CG736_RS11665 + CG736_RS11660

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 5, location 121834 - 194362:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal) + (Acetyl-CoA - Me-redmal) + (ohmal) + (Ser) + (ccmal) + (Gly - Tyr)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 6, location 147608 - 217805:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Acetyl-CoA - Me-redmal) + (ohmal) + (Ser) + (ccmal) + (Gly - Tyr)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 7, location 147608 - 209994:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Acetyl-CoA - Me-redmal) + (ohmal) + (Ser) + (ccmal) + (Gly - Tyr)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 8, location 213724 - 306145:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk - Orn)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 10, location 219115 - 306145:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk - Orn)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

CG736_RS11660: mmal

PKS_AT (552..845): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 210.3
Ethylmalonyl-CoA: 190.8
Methoxymalonyl-CoA: 159.6
2-Methylbutyryl-CoA: 125.2
Malonyl-CoA: 109.2
trans-1,2-CPDA: 101.9
Propionyl-CoA: 96.2
CHC-CoA: 87.8
Benzoyl-CoA: 85.5
Isobutyryl-CoA: 80.2
Acetyl-CoA: 50.9
3-Methylbutyryl-CoA: 38.2
inactive: 38.0
fatty_acid: 23.5

CG736_RS11665: mal

PKS_AT (538..822): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 62.5%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 143.1
Methoxymalonyl-CoA: 77.6
Methylmalonyl-CoA: 62.0
inactive: 53.4
Propionyl-CoA: 32.4
Ethylmalonyl-CoA: 27.3
Isobutyryl-CoA: 18.0
CHC-CoA: 16.2
Acetyl-CoA: 14.8
Benzoyl-CoA: 12.2
2-Methylbutyryl-CoA: 9.9
trans-1,2-CPDA: 9.1
fatty_acid: 6.0
3-Methylbutyryl-CoA: 0.0

CG736_RS11670: mal

PKS_AT (14..291): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 66.7%; Methylmalonyl-CoA: 58.3%; inactive: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 51.7
Methoxymalonyl-CoA: 42.6
Methylmalonyl-CoA: 42.3
Propionyl-CoA: 20.0
fatty_acid: 18.4
inactive: 18.2
Isobutyryl-CoA: 16.7
2-Methylbutyryl-CoA: 16.5
CHC-CoA: 12.8
Benzoyl-CoA: 11.8
trans-1,2-CPDA: 9.3
Ethylmalonyl-CoA: 8.2
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

CG736_RS11770: mal

PKS_AT (113..401): mal

ATSignature: (unknown)

No matches above 50%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 19.2
Propionyl-CoA: 19.1
Methylmalonyl-CoA: 17.3
Methoxymalonyl-CoA: 9.4
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

CG736_RS11795: X

AMP-binding (35..413): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: GQLLHPMHLN-AYTSTGGALQGYGLSETSSLATS
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

CG736_RS11820: mal

PKS_AT (16..298): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 66.7%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 43.2
Methylmalonyl-CoA: 31.6
Methoxymalonyl-CoA: 28.4
fatty_acid: 23.7
inactive: 14.7
Isobutyryl-CoA: 10.7
Benzoyl-CoA: 8.8
Propionyl-CoA: 7.9
trans-1,2-CPDA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

CG736_RS11880: Acetyl-CoA - mmal

CAL_domain (45..493): Acetyl-CoA

minowa: Acetyl-CoA

Prediction, score:

Acetyl-CoA: 74.8
NH2: 31.9
fatty_acid: 23.5
AHBA: 11.4
shikimic_acid: 0.0

PKS_AT (1261..1559): mmal

ATSignature: Malonyl-CoA, Methylmalonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; Methylmalonyl-CoA: 70.8%; Propionyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 119.9
Methoxymalonyl-CoA: 100.2
Malonyl-CoA: 99.9
Isobutyryl-CoA: 79.5
Ethylmalonyl-CoA: 75.4
Propionyl-CoA: 72.7
2-Methylbutyryl-CoA: 59.1
Benzoyl-CoA: 47.3
CHC-CoA: 36.1
Acetyl-CoA: 36.1
3-Methylbutyryl-CoA: 34.3
trans-1,2-CPDA: 33.4
inactive: 30.9
fatty_acid: 21.8

CG736_RS11885: mal

PKS_AT (558..858): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 66.7%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 151.4
inactive: 105.6
Methylmalonyl-CoA: 68.7
Methoxymalonyl-CoA: 68.0
Isobutyryl-CoA: 40.9
Propionyl-CoA: 40.3
2-Methylbutyryl-CoA: 37.5
Ethylmalonyl-CoA: 25.4
CHC-CoA: 24.3
Acetyl-CoA: 22.5
trans-1,2-CPDA: 21.1
Benzoyl-CoA: 18.9
fatty_acid: 9.8
3-Methylbutyryl-CoA: 8.0

CG736_RS11890: Ser

Search NORINE for peptide: strict or relaxed

AMP-binding (498..896): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDVSVWEWHFVCSGEHNLYGPTEAAVDVTA
Nearest Stachelhaus code(s):: Ser DVWHVSLVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

CG736_RS11895: mal

PKS_AT (540..834): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 169.9
inactive: 89.1
Methylmalonyl-CoA: 68.0
Methoxymalonyl-CoA: 67.9
Propionyl-CoA: 51.7
Ethylmalonyl-CoA: 42.1
2-Methylbutyryl-CoA: 36.8
CHC-CoA: 33.6
Isobutyryl-CoA: 21.0
Benzoyl-CoA: 20.2
Acetyl-CoA: 19.0
trans-1,2-CPDA: 18.2
fatty_acid: 18.0
3-Methylbutyryl-CoA: 0.0

CG736_RS11900: Gly - Tyr

Search NORINE for peptide: strict or relaxed

AMP-binding (495..891): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: FSMTFDIAMLELQALVGGEWNMYGPTETTIWSSV
Nearest Stachelhaus code(s):: Gly DILQLGMIWK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1564..1960): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LGQAFDAAVAEVTLVTAGEINAYGPTETTVCATM
Nearest Stachelhaus code(s):: Tyr DAATVAAVCK (85% 8Å match)
Stachelhaus code match:: 100% (strong)

CG736_RS12250: pk - Orn

Search NORINE for peptide: strict or relaxed

PKS_AT (547..843): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; CE-Malonyl-CoA: 58.3%; Acetyl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 110.4
Methoxymalonyl-CoA: 109.6
Ethylmalonyl-CoA: 95.6
Malonyl-CoA: 94.0
Isobutyryl-CoA: 87.5
trans-1,2-CPDA: 68.2
Acetyl-CoA: 61.5
Propionyl-CoA: 57.8
Benzoyl-CoA: 56.7
2-Methylbutyryl-CoA: 55.7
fatty_acid: 43.4
CHC-CoA: 40.8
3-Methylbutyryl-CoA: 29.6
inactive: 27.0

AMP-binding (2271..2677): Orn

nrpys: Orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Long positively charged side chain (Orn, Lys, Arg)
Small clusters prediction:: Orn and hydroxy-Orn specific (Orn, OH-Orn)
Single AA prediction:: orn (Orn)

Stachelhaus prediction details:

Stachelhaus sequence:: VGWAFDVFTGDREFIVGSDVNSYGLTEATIDSAW
Nearest Stachelhaus code(s):: Orn DVGEIGSIDK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor

Type II PKS gene cluster prediction details

Cluster section:: 71461 to 143938
Starter unit(s):: hexadienyl-CoA (Score: 640.0; E-value: 8.77e-142)
2-methylbutyryl-CoA (Score: 387.0; E-value: 4.71e-138)
Product class(es):: benzoisochromanequinone

CG736_RS11440: KR (Score: 100.6; E-value: 2.6e-31)
CG736_RS11515: KR (Score: 54.6; E-value: 2.9e-17)
CG736_RS11600: CYC C5-C14 (Score: 473.4; E-value: 5.7e-145)
CG736_RS11625: KS (Score: 731.7; E-value: 5.2e-223)
CG736_RS11630: CLF (Score: 586.9; E-value: 4.4e-179)
CG736_RS11650: CYC C7-C12 (Score: 406.7; E-value: 1.2e-124); ACP (Score: 80.2; E-value: 2e-25)
CG736_RS11655: KR C9 (Score: 433.0; E-value: 8.7e-133)
CG736_RS11660: AT (Score: 157.8; E-value: 8.1e-49); AT ADG86309.1_A-74528_hexadienyl-CoA (Score: 112.0; E-value: 1.32e-29)
CG736_RS11665: AT (Score: 184.0; E-value: 8.5e-57); AT ADG86309.1_A-74528_hexadienyl-CoA (Score: 133.0; E-value: 3.83e-37)
CG736_RS11670: AT (Score: 411.6; E-value: 5.7e-126); AT AAP85359.1_Hedamycin_hexadienyl-CoA (Score: 395.0; E-value: 8.77e-142)
CG736_RS11675: KSIII (Score: 421.0; E-value: 7.9e-129); KSIII ADB23398.1_TLN-05220/TLN-05223_2-methylbutyryl-CoA (Score: 387.0; E-value: 4.71e-138)
CG736_RS11700: CYC (Score: 17.4; E-value: 5.5e-06)
CG736_RS11725: OXY (Score: 47.0; E-value: 4.4e-15)

Cluster section:: 121834 to 194362
Starter unit(s):: propionyl-CoA (Score: 1003.6; E-value: 2.1e-139)
2-methylbutyryl-CoA (Score: 591.0; E-value: 4.71e-138)
hexadienyl-CoA (Score: 395.0; E-value: 8.77e-142)
Malonyl elongations:: 8|9 (Score: 672.6; E-value: 3.9e-205)
Product molecular weight(s):: 2-methylbutyryl-CoA_8: 379 Da
2-methylbutyryl-CoA_9: 421 Da
hexadienyl-CoA_8: 389 Da
hexadienyl-CoA_9: 431 Da
propionyl-CoA_8: 351 Da
propionyl-CoA_9: 393 Da

CG736_RS11670: AT (Score: 411.6; E-value: 5.7e-126); AT AAP85359.1_Hedamycin_hexadienyl-CoA (Score: 395.0; E-value: 8.77e-142)
CG736_RS11675: KSIII (Score: 421.0; E-value: 7.9e-129); KSIII ADB23398.1_TLN-05220/TLN-05223_2-methylbutyryl-CoA (Score: 387.0; E-value: 4.71e-138)
CG736_RS11700: CYC (Score: 17.4; E-value: 5.5e-06)
CG736_RS11725: OXY (Score: 47.0; E-value: 4.4e-15)
CG736_RS11765: ACP (Score: 56.6; E-value: 4.4e-18)
CG736_RS11770: AT (Score: 255.9; E-value: 1.2e-78); AT ADB23379.1_TLN-05220/TLN-05223_2-methylbutyryl-CoA (Score: 204.0; E-value: 1.16e-62)
CG736_RS11785: CYC C5-C14/C3-C16 (Score: 407.7; E-value: 4.4e-125)
CG736_RS11805: KR (Score: 194.1; E-value: 8e-60)
CG736_RS11810: CYC C7-C12 (Score: 401.9; E-value: 3.3e-123)
CG736_RS11815: KR C9 (Score: 449.7; E-value: 6.9e-138)
CG736_RS11820: AT (Score: 412.6; E-value: 2.8e-126); AT BAB72049.1_Aklavinone_propionyl-CoA (Score: 309.0; E-value: 3.19e-107)
CG736_RS11825: ACP (Score: 103.5; E-value: 1e-32)
CG736_RS11830: CLF 8|9 (Score: 672.6; E-value: 3.9e-205)
CG736_RS11835: KS (Score: 762.4; E-value: 2.5e-232)
CG736_RS11840: KSIII (Score: 436.7; E-value: 1.3e-133); KSIII ABC00733.1_Cosmomycin_propionyl-CoA (Score: 390.0; E-value: 2.1e-139)
CG736_RS11880: KR (Score: 42.8; E-value: 1.2e-13)
CG736_RS11885: AT (Score: 189.5; E-value: 1.9e-58); AT ABC00730.1_Cosmomycin_propionyl-CoA (Score: 128.0; E-value: 4.05e-35)
CG736_RS11895: AT (Score: 255.3; E-value: 1.8e-78); AT BAB72049.1_Aklavinone_propionyl-CoA (Score: 155.0; E-value: 4.69e-44); AT AAF70110.1_Aclacinomycin_propionyl-CoA (Score: 21.6; E-value: 3.9)

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
AfsR	Pleiotropic regulatory for antibiotic production
CRP	Cyclic AMP receptor protein
CelR	Cellobiose uptake repressor
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
DmdR1	Iron(II)-dependent repressor
IolR	Regulator of the inositol catabolism
OsdR	Development and stress management regulator
VqsM	Regulator of quorum-sensing signalling systems

NZ_NNBO01000005.1 - Region 1 - azole-containing-RiPP

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Location: 7,774 - 42,598 nt. (total: 34,825 nt) Show pHMM detection rules used

azole-containing-RiPP: ((YcaO or TIGR03604) and (((thio_amide and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or TIGR03603 or thiostrepton or TIGR03602)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Azole RiPPs

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidase

CG736_RS13385: FAD linked oxidase domain protein

smcogs: SMCOG1138: FAD linked oxidase domain protein

Hydrolases: hydrolase

CG736_RS13340: amidohydrolase

smcogs: SMCOG1244: amidohydrolase

Lyases: dehydratase

CG736_RS13305: Lantibiotic dehydratase domain protein

smcogs: SMCOG1155: Lantibiotic dehydratase domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001146.5	0.53	ribosomal	cyclothiazomycin C	Streptomyces sp. NRRL WC-3908
BGC0001145.5	0.53	ribosomal	cyclothiazomycin b1	Streptomyces mobaraensis NBRC 13819 = DSM 40847
BGC0000603.5	0.49	ribosomal	cyclothiazomycin	Streptomyces hygroscopicus
BGC0001860.4	0.49	ribosomal	thiomuracin	Thermobispora bispora DSM 43833
BGC0000613.5	0.48	ribosomal	thiomuracin A	Nonomuraea sp. Bp3714-39
BGC0001640.3	0.47	ribosomal	kocurin	Kocuria rosea
BGC0001472.5	0.47	ribosomal	berninamycin A	Streptomyces bernensis
BGC0000606.5	0.47	ribosomal	lactazole	Streptomyces lactacystinaeus
BGC0001174.4	0.47	ribosomal	corynazolicin	Corynebacterium urealyticum DSM 7111
BGC0002120.3	0.46	ribosomal	nocathioamide A	Nocardia terpenica IFM 0706

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001146.5	0.53	ribosomal	cyclothiazomycin C	Streptomyces sp. NRRL WC-3908
BGC0001145.5	0.53	ribosomal	cyclothiazomycin b1	Streptomyces mobaraensis NBRC 13819 = DSM 40847
BGC0000603.5	0.49	ribosomal	cyclothiazomycin	Streptomyces hygroscopicus
BGC0001860.4	0.49	ribosomal	thiomuracin	Thermobispora bispora DSM 43833
BGC0000613.5	0.48	ribosomal	thiomuracin A	Nonomuraea sp. Bp3714-39
BGC0001640.3	0.47	ribosomal	kocurin	Kocuria rosea
BGC0001472.5	0.47	ribosomal	berninamycin A	Streptomyces bernensis
BGC0000606.5	0.47	ribosomal	lactazole	Streptomyces lactacystinaeus
BGC0001174.4	0.47	ribosomal	corynazolicin	Corynebacterium urealyticum DSM 7111
BGC0002120.3	0.46	ribosomal	nocathioamide A	Nocardia terpenica IFM 0706

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 21.16 out of 26.33

Confidence: medium

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 17.11 out of 19.52

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.17 out of 23.79

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.46 out of 23.05

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.76 out of 23.05

Confidence: medium

Additional weak hits (17):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 17.99 out of 26.33

Confidence: weak

DosR

Description: Hypoxia stress response regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 23.51 out of 29.51

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.41 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 12.46 out of 28.14

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 12.62 out of 26.06

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.18 out of 26.33

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 20.07 out of 22.40

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.24 out of 28.19

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.98 out of 20.01

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.26 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 12.53 out of 26.06

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 16.79 out of 36.54

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.50 out of 30.22

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.40 out of 21.84

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 22.78 out of 30.98

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 21.07 out of 27.04

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.27 out of 29.65

Confidence: weak

(Thi)azol(in)e containing peptide predictions

No core peptides found.

RREFinder

TFBS Finder

Azole RiPPs

RRE predictions

: RRE type: Thiopeptide_F_RRE; Location: 9 - 88; Score: 55.4; RRE type: Heterocycloanthracin_C_RRE; Location: 8 - 91; Score: 41.3

TFBS definitions

Regulator	Description
ANR	Anaerobic transcriptional regulator
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
AfsR	Pleiotropic regulatory for antibiotic production
IolR	Regulator of the inositol catabolism

Core peptide predictions

No core peptides found.

NZ_NNBO01000005.1 - Region 2 - NRPS,T1PKS,transAT-PKS

Download region GenBank file

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Location: 247,603 - 417,137 nt. (total: 169,535 nt) Show pHMM detection rules used

transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

CG736_RS14315: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

CG736_RS14375: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

CG736_RS14400: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

CG736_RS14440: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

CG736_RS14480: alcohol dehydrogenase

smcogs: SMCOG1040: alcohol dehydrogenase

CG736_RS14550: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

CG736_RS14600: 3-hydroxybutyryl-CoA dehydrogenase

smcogs: SMCOG1095: 3-hydroxybutyryl-CoA dehydrogenase

CG736_RS14610: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

Transferases: PPTase acylase aminotransferase

CG736_RS14390: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

CG736_RS14430: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1012: 4'-phosphopantetheinyl transferase

CG736_RS14500: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

CG736_RS14575: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

CG736_RS14635: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: esterase hydrolase phosphatase

CG736_RS14380: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

CG736_RS14425: thioesterase

smcogs: SMCOG1004: thioesterase

CG736_RS14505: inositol monophosphatase

smcogs: SMCOG1207: inositol monophosphatase

CG736_RS14615: FkbH like protein

smcogs: SMCOG1256: FkbH like protein

CG736_RS14630: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: dehydratase hydratase

CG736_RS14385: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

CG736_RS38600: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: ligase synthase

CG736_RS14355: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

CG736_RS14420: hydroxymethylglutaryl-CoA synthase

smcogs: SMCOG1043: hydroxymethylglutaryl-CoA synthase

CG736_RS14460: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

CG736_RS14560: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

CG736_RS14565: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

CG736_RS14570: 8-amino-7-oxononanoate synthase

smcogs: SMCOG1109: 8-amino-7-oxononanoate synthase

CG736_RS14580: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

CG736_RS14585: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

CG736_RS14590: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001641.4	0.85	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002276.2	0.77	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0000028.5	0.73	PKS	bafilomycin B1	Streptomyces lohii
BGC0000041.3	0.66	PKS	oronofacic acid	Pseudomonas syringae
BGC0001050.5	0.64	NRPS, PKS	thalassospiramide A	Tistrella bauzanensis
BGC0002135.2	0.59	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000307.4	0.59	NRPS	AbT1	Aureobasidium pullulans
BGC0001261.3	0.59	NRPS	AM-toxin	Alternaria alternata
BGC0001250.4	0.58	NRPS	epichloenin A	Epichloe festucae
BGC0001251.4	0.58	NRPS	epichloenin A	Epichloe festucae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000028.5	0.81	PKS	bafilomycin B1	Streptomyces lohii
BGC0001276.3	0.73	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.73	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.73	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.73	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000041.3	0.69	PKS	oronofacic acid	Pseudomonas syringae
BGC0001348.5	0.65	PKS	JBIR-100	Streptomyces varsoviensis
BGC0001641.4	0.64	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.64	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0000067.5	0.64	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus

Similar known clusters from MIBiG 4.0

Similar subclusters

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.88 out of 20.47

Confidence: medium

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 23.69 out of 30.25

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.42 out of 20.47

Confidence: medium

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 23.33 out of 29.72

Confidence: medium

Additional weak hits (32):

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 12.39 out of 28.14

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.07 out of 29.38

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.69 out of 27.78

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.73 out of 30.25

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.12 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.57 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 12.07 out of 26.06

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 13.71 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.49 out of 26.06

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 16.11 out of 31.73

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.64 out of 27.78

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.91 out of 30.25

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 22.19 out of 30.22

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.54 out of 30.25

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.18 out of 28.19

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 21.50 out of 36.53

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 12.79 out of 26.06

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.83 out of 29.38

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 16.89 out of 29.25

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 20.16 out of 32.74

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.99 out of 29.38

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 17.23 out of 24.78

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.47 out of 28.19

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 20.22 out of 30.98

Confidence: weak

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 16.79 out of 19.52

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.79 out of 29.25

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 20.80 out of 30.22

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 15.40 out of 33.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.26 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.28 out of 28.19

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 20.96 out of 36.53

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.58 out of 32.93

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 2, location 247602 - 417137:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Cys - mal - ccmal - ccmal) + (mal - ccmal - ccmal - mal) + (emal - Me-ohmal - ohmal - Me-mal) + (Me-ohmal - ohemal - ccmal) + (pk - Me-redmal) + (Me-ohmal - Me-ccmal) + (Me-ccmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 247602 - 326396:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Cys - mal - ccmal - ccmal) + (mal - ccmal - ccmal - mal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 319506 - 417137:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (emal - Me-ohmal - ohmal - Me-mal) + (Me-ohmal - ohemal - ccmal) + (pk - Me-redmal) + (Me-ohmal - Me-ccmal) + (Me-ccmal)
Non-colinear ordering:: CG736_RS14575 + CG736_RS14580 + CG736_RS14585 + CG736_RS14590 + CG736_RS14595

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

CG736_RS14355: Ala

Search NORINE for peptide: strict or relaxed

AMP-binding (21..427): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: FAVYFDLSMFEATSMFCGEDNLYGPTELAVACTG
Nearest Stachelhaus code(s):: D-Ala DLFTVCLIAK (82% 8Å match); Ala DLMTMCTVAK (62% 8Å match)
Stachelhaus code match:: 80% (moderate)

CG736_RS14390: mal

PKS_AT (4..282): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 62.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 117.4
Methoxymalonyl-CoA: 50.2
Propionyl-CoA: 40.4
Methylmalonyl-CoA: 35.2
inactive: 34.3
2-Methylbutyryl-CoA: 18.2
CHC-CoA: 12.9
fatty_acid: 12.8
Benzoyl-CoA: 6.7
Ethylmalonyl-CoA: 4.3
Isobutyryl-CoA: 3.0
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0
Acetyl-CoA: -3.2

CG736_RS14400: Cys

Search NORINE for peptide: strict or relaxed

AMP-binding (991..1396): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: LWCTFDLSVFDGNSFLSGDISLGGATEATVWSNW
Nearest Stachelhaus code(s):: Cys DLFNFSLVWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

CG736_RS14460: X

AMP-binding (14..397): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: APPAITASATTMMMATASAVGAYSASQAKPAVTI
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

CG736_RS14560: X

AMP-binding (10..412): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: NTTLHTFGICGTMGFSGGALEGYGLTEASPVVAY
Nearest Stachelhaus code(s):: nLev GFCMYGGCSK (71% 8Å match)
Stachelhaus code match:: 60% (weak)

CG736_RS14565: X

AMP-binding (15..418): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FAGLHSSGATADLLTYLGSLQVYATSEAGFVSML
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

CG736_RS14575: emal - mmal - mal - mmal

PKS_AT (99..398): emal

ATSignature: Isobutyryl-CoA

Top 3 matches:: Isobutyryl-CoA: 79.2%; Methoxymalonyl-CoA: 70.8%; Methylmalonyl-CoA: 70.8%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 160.7
Methylmalonyl-CoA: 160.5
2-Methylbutyryl-CoA: 152.4
Propionyl-CoA: 130.4
Methoxymalonyl-CoA: 130.3
CHC-CoA: 101.7
Malonyl-CoA: 85.9
trans-1,2-CPDA: 85.0
Isobutyryl-CoA: 83.9
Benzoyl-CoA: 52.6
3-Methylbutyryl-CoA: 35.6
Acetyl-CoA: 33.2
inactive: 19.8
fatty_acid: 14.4

PKS_AT (1102..1392): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 210.2
Ethylmalonyl-CoA: 198.2
Methoxymalonyl-CoA: 176.5
2-Methylbutyryl-CoA: 174.5
trans-1,2-CPDA: 134.6
Propionyl-CoA: 130.3
CHC-CoA: 111.1
Malonyl-CoA: 93.4
Isobutyryl-CoA: 75.1
Benzoyl-CoA: 64.6
fatty_acid: 58.2
Acetyl-CoA: 49.9
3-Methylbutyryl-CoA: 43.7
inactive: 24.2

PKS_AT (2666..2958): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 236.0
inactive: 116.7
Methoxymalonyl-CoA: 70.8
Methylmalonyl-CoA: 58.4
Propionyl-CoA: 45.3
Isobutyryl-CoA: 32.1
Ethylmalonyl-CoA: 22.9
Benzoyl-CoA: 17.8
2-Methylbutyryl-CoA: 16.8
Acetyl-CoA: 16.0
CHC-CoA: 11.1
trans-1,2-CPDA: 9.2
fatty_acid: 9.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (4194..4490): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Malonyl-CoA: 87.5%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 202.6
Ethylmalonyl-CoA: 197.1
Methoxymalonyl-CoA: 167.3
2-Methylbutyryl-CoA: 142.7
trans-1,2-CPDA: 118.3
Propionyl-CoA: 110.5
CHC-CoA: 95.5
Malonyl-CoA: 88.8
Isobutyryl-CoA: 79.9
Benzoyl-CoA: 66.9
Acetyl-CoA: 48.6
fatty_acid: 42.7
3-Methylbutyryl-CoA: 42.4
inactive: 22.7

CG736_RS14580: mmal - emal - mal

PKS_AT (564..861): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 220.5
Ethylmalonyl-CoA: 208.7
Methoxymalonyl-CoA: 181.7
2-Methylbutyryl-CoA: 167.4
trans-1,2-CPDA: 129.0
Propionyl-CoA: 123.7
CHC-CoA: 112.8
Malonyl-CoA: 92.7
Isobutyryl-CoA: 85.2
Benzoyl-CoA: 73.1
Acetyl-CoA: 59.4
3-Methylbutyryl-CoA: 43.9
fatty_acid: 42.9
inactive: 27.9

PKS_AT (2122..2420): emal

ATSignature: Ethylmalonyl-CoA, Methylmalonyl-CoA, Methoxymalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 95.8%; Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 95.8%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 216.3
Methylmalonyl-CoA: 198.0
Methoxymalonyl-CoA: 179.9
trans-1,2-CPDA: 146.0
2-Methylbutyryl-CoA: 124.9
Propionyl-CoA: 102.1
CHC-CoA: 87.2
Malonyl-CoA: 82.6
Benzoyl-CoA: 61.2
Isobutyryl-CoA: 60.5
Acetyl-CoA: 34.2
3-Methylbutyryl-CoA: 32.8
fatty_acid: 27.3
inactive: 22.0

PKS_AT (3684..3984): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 75.0%; 2-Methylbutyryl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 207.8
inactive: 95.9
Methoxymalonyl-CoA: 58.3
Methylmalonyl-CoA: 50.3
Propionyl-CoA: 30.0
Ethylmalonyl-CoA: 24.0
Acetyl-CoA: 21.3
Isobutyryl-CoA: 17.5
CHC-CoA: 12.6
2-Methylbutyryl-CoA: 12.0
Benzoyl-CoA: 8.2
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

CG736_RS14585: pk - mmal

PKS_AT (564..862): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 227.2
Methylmalonyl-CoA: 226.7
Methoxymalonyl-CoA: 189.8
2-Methylbutyryl-CoA: 182.0
trans-1,2-CPDA: 148.6
Propionyl-CoA: 127.7
Malonyl-CoA: 126.0
CHC-CoA: 120.6
Benzoyl-CoA: 80.1
Isobutyryl-CoA: 79.0
Acetyl-CoA: 45.1
3-Methylbutyryl-CoA: 43.8
inactive: 33.2
fatty_acid: 31.6

PKS_AT (2324..2614): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 211.0
Ethylmalonyl-CoA: 199.9
Methoxymalonyl-CoA: 171.7
2-Methylbutyryl-CoA: 150.9
trans-1,2-CPDA: 127.0
Propionyl-CoA: 115.2
CHC-CoA: 96.7
Isobutyryl-CoA: 87.0
Malonyl-CoA: 86.2
Benzoyl-CoA: 71.4
Acetyl-CoA: 56.9
3-Methylbutyryl-CoA: 52.4
fatty_acid: 42.3
inactive: 20.4

CG736_RS14590: mmal - mmal

PKS_AT (569..868): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 220.3
Ethylmalonyl-CoA: 217.6
Methoxymalonyl-CoA: 184.2
2-Methylbutyryl-CoA: 157.2
trans-1,2-CPDA: 116.8
Propionyl-CoA: 113.8
CHC-CoA: 113.8
Malonyl-CoA: 110.3
Isobutyryl-CoA: 83.6
Benzoyl-CoA: 76.7
Acetyl-CoA: 53.2
3-Methylbutyryl-CoA: 51.6
inactive: 44.4
fatty_acid: 38.5

PKS_AT (2120..2411): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 212.9
Ethylmalonyl-CoA: 202.5
Methoxymalonyl-CoA: 178.0
2-Methylbutyryl-CoA: 158.6
trans-1,2-CPDA: 122.0
Propionyl-CoA: 116.2
CHC-CoA: 103.4
Malonyl-CoA: 90.1
Isobutyryl-CoA: 89.1
Benzoyl-CoA: 71.9
Acetyl-CoA: 56.0
3-Methylbutyryl-CoA: 48.3
fatty_acid: 47.0
inactive: 25.4

CG736_RS14595: mmal

PKS_AT (566..858): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 201.0
Ethylmalonyl-CoA: 193.9
Methoxymalonyl-CoA: 171.7
2-Methylbutyryl-CoA: 158.4
trans-1,2-CPDA: 131.9
Propionyl-CoA: 113.1
CHC-CoA: 100.4
Malonyl-CoA: 97.9
Isobutyryl-CoA: 67.2
Benzoyl-CoA: 66.9
fatty_acid: 59.6
3-Methylbutyryl-CoA: 42.4
Acetyl-CoA: 38.9
inactive: 28.5

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
ColR	Phenol-responsive regulator
NtrC	Nitrogen regulatory protein C
VqsM	Regulator of quorum-sensing signalling systems

NZ_NNBO01000005.1 - Region 3 - terpene

Download region GenBank file

Download region SVG

Location: 459,866 - 482,685 nt. (total: 22,820 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

CG736_RS14925: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Hydrolases: hydrolase

CG736_RS14850: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Isomerases: mutase

CG736_RS14890: phosphoglycerate mutase

smcogs: SMCOG1101: phosphoglycerate mutase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000244.4	0.45	PKS	nonactin, monactin, dinactin, trinactin, tetranactin	Streptomyces griseus subsp. griseus
BGC0001928.2	0.39	other (other)	(2R,3S,4S)-5-fluoro-2,3,4-trihydroxypentanoic acid	Streptomyces sp. MA37
BGC0000626.3	0.36	ribosomal	thuricin	Bacillus thuringiensis
BGC0000519.4	0.36	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0002218.2	0.36	terpene	(-)-Cyatha-3,12-diene	Dentipellis sp. KUC8613
BGC0000501.3	0.36	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0002635.2	0.36	ribosomal	daspyromycin A, daspyromycin B	Actinokineospora diospyrosa
BGC0000643.3	0.34	terpene	carotenoid	Brevundimonas vesicularis
BGC0000497.3	0.34	ribosomal	BHT-Aα, BHT-Aβ	Streptococcus ratti
BGC0001341.4	0.33	NRPS	valinomycin	Rothia nasimurium

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000244.4	0.45	PKS	nonactin, monactin, dinactin, trinactin, tetranactin	Streptomyces griseus subsp. griseus
BGC0001928.2	0.39	other (other)	(2R,3S,4S)-5-fluoro-2,3,4-trihydroxypentanoic acid	Streptomyces sp. MA37
BGC0000626.3	0.36	ribosomal	thuricin	Bacillus thuringiensis
BGC0000519.4	0.36	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0002218.2	0.36	terpene	(-)-Cyatha-3,12-diene	Dentipellis sp. KUC8613
BGC0000501.3	0.36	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0002635.2	0.36	ribosomal	daspyromycin A, daspyromycin B	Actinokineospora diospyrosa
BGC0000643.3	0.34	terpene	carotenoid	Brevundimonas vesicularis
BGC0000497.3	0.34	ribosomal	BHT-Aα, BHT-Aβ	Streptococcus ratti
BGC0001341.4	0.33	NRPS	valinomycin	Rothia nasimurium

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

No product predictions

Genes with terpene domains:

No domains found

Transcription Factor Binding Sites

Binding sites found:

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 18.33 out of 19.52

Confidence: strong

Additional weak hits (5):

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.83 out of 26.85

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 19.23 out of 33.14

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.27 out of 20.01

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.35 out of 30.25

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.59 out of 22.40

Confidence: weak

Terpene

TFBS Finder

Glossary

No acronyms to display

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production

NZ_NNBO01000006.1 - Region 1 - terpene

Download region GenBank file

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Location: 16,703 - 37,746 nt. (total: 21,044 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

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KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: glucosidase hydrolase

CG736_RS15185: Alpha-glucosidase

smcogs: SMCOG1257: Alpha-glucosidase

CG736_RS15225: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

Lyases: cyclase

CG736_RS15215: Terpene synthase/cyclase metal-binding domain protein

smcogs: SMCOG1052: Terpene synthase/cyclase metal-binding domain protein

Translocases: transporter

CG736_RS15200: sugar-binding lipoprotein

smcogs: SMCOG1143: sugar-binding lipoprotein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002285.2	0.54	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0002582.3	0.38	NRPS	guanipiperazine A, guanipiperazine B	Streptomyces chrestomyceticus
BGC0000804.5	0.38	saccharide	acarviostatin I03, acarviostatin II03, acarviostatin III03, acarviostatin IV03	Streptomyces coelicoflavus ZG0656
BGC0001510.5	0.35	other (other)	anisomycin	Streptomyces hygrospinosus
BGC0000709.5	0.34	saccharide	neomycin	Streptomyces fradiae ATCC 10745 = DSM 40063
BGC0000711.5	0.33	saccharide	neomycin	Streptomyces fradiae
BGC0000495.5	0.32	ribosomal	actagardine	Actinoplanes garbadinensis
BGC0001011.5	0.28	NRPS, PKS	meridamycin	Streptomyces sp. NRRL 30748
BGC0001925.4	0.28	other (other)	altemicidin, SB-203207, SB-203208	Streptomyces sp.
BGC0002454.2	0.27	PKS	venturicidin D, venturicidin E, venturicidin F, venturicidin A	Streptomyces sp. NRRL S-4

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002285.2	0.54	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0002582.3	0.38	NRPS	guanipiperazine A, guanipiperazine B	Streptomyces chrestomyceticus
BGC0000804.5	0.38	saccharide	acarviostatin I03, acarviostatin II03, acarviostatin III03, acarviostatin IV03	Streptomyces coelicoflavus ZG0656
BGC0001510.5	0.35	other (other)	anisomycin	Streptomyces hygrospinosus
BGC0000709.5	0.34	saccharide	neomycin	Streptomyces fradiae ATCC 10745 = DSM 40063
BGC0000711.5	0.33	saccharide	neomycin	Streptomyces fradiae
BGC0000495.5	0.32	ribosomal	actagardine	Actinoplanes garbadinensis
BGC0001011.5	0.28	NRPS, PKS	meridamycin	Streptomyces sp. NRRL 30748
BGC0001925.4	0.28	other (other)	altemicidin, SB-203207, SB-203208	Streptomyces sp.
BGC0002454.2	0.27	PKS	venturicidin D, venturicidin E, venturicidin F, venturicidin A	Streptomyces sp. NRRL S-4

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

No product predictions

Genes with terpene domains:

CG736_RS15215: T1TS

Domain 1 (4 - 309): Type 1 terpene synthase

Subtype: unknown

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.92 out of 20.47

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.75 out of 23.79

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.74 out of 20.47

Confidence: medium

Additional weak hits (7):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 18.03 out of 27.78

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.69 out of 20.97

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.01 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.61 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.61 out of 28.14

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.52 out of 24.78

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.41 out of 28.19

Confidence: weak

Terpene

TFBS Finder

Glossary

No acronyms to display

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism
VqsM	Regulator of quorum-sensing signalling systems

NZ_NNBO01000006.1 - Region 2 - T3PKS,terpene

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Location: 41,294 - 103,121 nt. (total: 61,828 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)
T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

CG736_RS15310: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

CG736_RS15360: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

CG736_RS15395: dehydrogenase

smcogs: SMCOG1181: dehydrogenase

CG736_RS15465: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

CG736_RS15495: oxidoreductase

smcogs: SMCOG1161: oxidoreductase

CG736_RS15500: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

CG736_RS15545: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

CG736_RS15550: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

CG736_RS15555: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

CG736_RS15560: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

CG736_RS15580: pyridine nucleotide-disulfide oxidoreductase

smcogs: SMCOG1175: pyridine nucleotide-disulfide oxidoreductase

Transferases: acetyltransferase aminotransferase methyltransferase transferase

CG736_RS15330: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

CG736_RS15400: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

CG736_RS15475: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

CG736_RS15490: Methylation

MITE: MITE0000155Methylation (identity: 72.20%)
smcogs: SMCOG1150: isoprenylcysteine carboxyl methyltransferase

Hydrolases: glucosidase

CG736_RS15455: Alpha-glucosidase

smcogs: SMCOG1257: Alpha-glucosidase

Ligases: synthase synthetase

CG736_RS15335: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

CG736_RS15355: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

CG736_RS15485: chalcone and stilbene synthase domain protein

smcogs: SMCOG1024: chalcone and stilbene synthase domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000282.3	0.55	PKS	2-methoxy-5-methyl-6-(13-methyltetradecyl)-1,4-benzoquinone, 2-methoxy-5-methyl-6-(13-methyltetradecyl)phenol	Streptomyces griseus subsp. griseus NBRC 13350
BGC0002276.2	0.45	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0000663.5	0.36	terpene	hopene	Streptomyces coelicolor A3(2)
BGC0001928.2	0.33	other (other)	(2R,3S,4S)-5-fluoro-2,3,4-trihydroxypentanoic acid	Streptomyces sp. MA37
BGC0001361.3	0.32	terpene	sodorifen	Serratia plymuthica 4Rx13
BGC0002283.2	0.32	terpene	sodorifen	Serratia plymuthica
BGC0002554.2	0.30	other (other)	carquinostatin A, carquinostatin B	Streptomyces exfoliatus
BGC0000678.5	0.29	terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0001903.3	0.29	other (other)	neocarazostatin A	Streptomyces sp. MA37
BGC0000677.5	0.29	terpene	cyclooctatin	Streptomyces melanosporofaciens

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000282.3	0.81	PKS	2-methoxy-5-methyl-6-(13-methyltetradecyl)-1,4-benzoquinone, 2-methoxy-5-methyl-6-(13-methyltetradecyl)phenol	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000663.5	0.69	terpene	hopene	Streptomyces coelicolor A3(2)
BGC0000647.3	0.58	terpene	carotenoid	Rhodobacter sphaeroides
BGC0002276.2	0.56	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0001361.3	0.54	terpene	sodorifen	Serratia plymuthica 4Rx13
BGC0002283.2	0.54	terpene	sodorifen	Serratia plymuthica
BGC0002043.3	0.54	ribosomal	cittilin A, cittilin B	Myxococcus xanthus DK 1622
BGC0000660.5	0.53	terpene	albaflavenone	Streptomyces coelicolor A3(2)
BGC0001831.3	0.53	PKS	alkylpyrone-407, alkylpyrone-393	Myxococcus xanthus DK 1622
BGC0002173.2	0.53	saccharide, terpene	fumihopaside A, compound 3, 21-β-H-hopane-3beta, 22-diol	Aspergillus fumigatus A1163

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
triterpene	hopanoid		C30	hopanoid skeleton	unknown
triterpene	polypodane		C30	polypodane skeleton	unknown

Genes with terpene domains:

CG736_RS15350: T2TS

Domain 1 (24 - 643): Type 2 terpene synthase

Subtype: Squalene substrate

Reactions:: squalene → hopanoid; squalene → polypodane

CG736_RS15355: PT_FPPS_like

Domain 1 (60 - 350): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

CG736_RS15365: PT_phytoene_like

Domain 1 (2 - 279): Prenyltransferase; Phytoene synthase like

Subtype: unknown

Reactions:: FPP → PSPP; FPP → squalene; FPP → diapophytoene; GGPP → phytoene; PSPP → hydroxysqualene

CG736_RS15370: PT_phytoene_like

Domain 1 (9 - 297): Prenyltransferase; Phytoene synthase like

Subtype: unknown

Reactions:: FPP → PSPP; FPP → squalene; FPP → diapophytoene; GGPP → phytoene; PSPP → hydroxysqualene

Transcription Factor Binding Sites

Binding sites found:

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 20.94 out of 23.43

Confidence: medium

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 19.52 out of 19.52

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.33 out of 23.79

Confidence: medium

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 16.82 out of 18.46

Confidence: medium

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 33.07 out of 36.53

Confidence: strong

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 20.94 out of 25.31

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

Additional weak hits (13):

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 17.46 out of 31.73

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 15.56 out of 21.84

Confidence: weak

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.69 out of 19.26

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.84 out of 26.33

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 19.81 out of 30.98

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 15.89 out of 33.14

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 19.45 out of 23.43

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 22.02 out of 30.22

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 19.59 out of 33.73

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 17.57 out of 24.78

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 19.90 out of 29.65

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.75 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.24 out of 28.19

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PSPP	Presqualene diphosphate

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
BldD	Development and antibiotic global regulator
CatR	H2O2-responsive repressor
DmdR1	Iron(II)-dependent repressor
IolR	Regulator of the inositol catabolism
RpoN	Regulator of nitrogen assimilation and virulence

NZ_NNBO01000006.1 - Region 3 - RiPP-like

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Location: 175,458 - 186,258 nt. (total: 10,801 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxygenase

CG736_RS15945: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: aminotransferase

CG736_RS15980: aminotransferase

smcogs: SMCOG1019: aminotransferase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000902.4	0.51	PKS	flaviolin	Saccharopolyspora erythraea NRRL 2338
BGC0002127.3	0.47	PKS	flaviolin, 1,3,6,8-tetrahydroxynaphthalene	Streptomyces coelicolor A3(2)
BGC0000844.5	0.45	other (other)	clavulanic acid	Streptomyces clavuligerus
BGC0001485.4	0.43	other (other)	chuangxinmycin	Actinoplanes tsinanensis
BGC0001224.5	0.42	other (other)	reductasporine	uncultured bacterium
BGC0000285.5	0.41	PKS, saccharide	flaviolin, flaviolin rhamnoside, 3,3'-diflaviolin	Saccharopolyspora erythraea
BGC0001336.5	0.36	other (other)	erdasporine A, erdasporine B, erdasporine C	uncultured bacterium
BGC0001223.5	0.36	other (other)	hydroxysporine	uncultured bacterium
BGC0001483.4	0.35	other (other)	5-isoprenylindole-3-carboxylate β-D-glycosyl ester	Streptomyces sp. RM-5-8
BGC0001157.6	0.35	ribosomal	bottromycin A2	Streptomyces scabiei 87.22

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000902.4	0.51	PKS	flaviolin	Saccharopolyspora erythraea NRRL 2338
BGC0002127.3	0.47	PKS	flaviolin, 1,3,6,8-tetrahydroxynaphthalene	Streptomyces coelicolor A3(2)
BGC0000844.5	0.45	other (other)	clavulanic acid	Streptomyces clavuligerus
BGC0001485.4	0.43	other (other)	chuangxinmycin	Actinoplanes tsinanensis
BGC0001224.5	0.42	other (other)	reductasporine	uncultured bacterium
BGC0000285.5	0.41	PKS, saccharide	flaviolin, flaviolin rhamnoside, 3,3'-diflaviolin	Saccharopolyspora erythraea
BGC0001336.5	0.36	other (other)	erdasporine A, erdasporine B, erdasporine C	uncultured bacterium
BGC0001223.5	0.36	other (other)	hydroxysporine	uncultured bacterium
BGC0001483.4	0.35	other (other)	5-isoprenylindole-3-carboxylate β-D-glycosyl ester	Streptomyces sp. RM-5-8
BGC0001157.6	0.35	ribosomal	bottromycin A2	Streptomyces scabiei 87.22

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 21.40 out of 23.05

Confidence: strong

Additional weak hits (3):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.97 out of 27.78

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.55 out of 32.74

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 25.33 out of 30.98

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production

NZ_NNBO01000008.1 - Region 1 - terpene

Download region GenBank file

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Location: 46,429 - 68,621 nt. (total: 22,193 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: acetyltransferase

CG736_RS19055: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

Lyases: cyclase

CG736_RS18985: Terpene synthase/cyclase metal-binding domain protein

smcogs: SMCOG1052: Terpene synthase/cyclase metal-binding domain protein

Translocases: permease

CG736_RS18945: phenylalanine-specific permease

smcogs: SMCOG1038: phenylalanine-specific permease

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001181.4	0.81	terpene	geosmin	Streptomyces coelicolor A3(2)
BGC0001746.3	0.75	terpene	pristinol	Streptomyces pristinaespiralis ATCC 25486
BGC0002318.2	0.42	ribosomal	canucin A, canucin B	Streptomyces canus
BGC0000528.5	0.40	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0000903.3	0.39	other (other)	fluorometabolite	Streptomyces cattleya
BGC0001561.4	0.38	NRPS	curacomycin	Streptomyces curacoi
BGC0000844.5	0.36	other (other)	clavulanic acid	Streptomyces clavuligerus
BGC0002316.2	0.36	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0000503.5	0.34	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0000306.5	0.34	NRPS	arylomycin	Streptomyces filamentosus NRRL 11379

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001181.4	0.81	terpene	geosmin	Streptomyces coelicolor A3(2)
BGC0001746.3	0.75	terpene	pristinol	Streptomyces pristinaespiralis ATCC 25486
BGC0002318.2	0.42	ribosomal	canucin A, canucin B	Streptomyces canus
BGC0000528.5	0.40	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0000903.3	0.39	other (other)	fluorometabolite	Streptomyces cattleya
BGC0001561.4	0.38	NRPS	curacomycin	Streptomyces curacoi
BGC0000844.5	0.36	other (other)	clavulanic acid	Streptomyces clavuligerus
BGC0002316.2	0.36	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0000503.5	0.34	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0000306.5	0.34	NRPS	arylomycin	Streptomyces filamentosus NRRL 11379

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene		germacradienol	C15	C1-C10	OH
sesquiterpene		geosmin	C12	C1-C10	OH

Genes with terpene domains:

CG736_RS18985: T1TS + T1TS

Domain 1 (2 - 326): Type 1 terpene synthase

Subtype: Geosmin synthase, N terminal domain

Reactions:: FPP → germacradienol

Domain 2 (336 - 698): Type 1 terpene synthase

Subtype: Geosmin synthase, C terminal domain

Reactions:: germacradienol → geosmin

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.25 out of 23.79

Confidence: medium

Additional weak hits (20):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 20.06 out of 26.33

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.50 out of 30.22

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.51 out of 21.84

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.16 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.74 out of 28.14

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.59 out of 26.85

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.92 out of 27.78

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.27 out of 30.25

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.67 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.62 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.29 out of 26.06

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.54 out of 28.19

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.27 out of 29.65

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 17.52 out of 24.78

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.94 out of 22.40

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 12.95 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.15 out of 26.06

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.51 out of 33.14

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.50 out of 29.65

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 19.59 out of 24.78

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
FPP	Farnesyl diphosphate

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism

NZ_NNBO01000009.1 - Region 1 - NAPAA,lanthipeptide-class-ii,lanthipeptide-class-iv

Download region GenBank file

Download region SVG

Location: 95,945 - 136,640 nt. (total: 40,696 nt) Show pHMM detection rules used

NAPAA: (AMP-binding and TIGR02353)
lanthipeptide-class-iv: (cds(LANC_like and Pkinase) and not micKC)
lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase

CG736_RS20870: pyridine nucleotide-disulfide oxidoreductase

smcogs: SMCOG1175: pyridine nucleotide-disulfide oxidoreductase

Lyases: cyclase

CG736_RS20895: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002174.2	0.96	NRPS	ε-Poly-L-lysine	Epichloe festucae
BGC0000528.5	0.55	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0001135.5	0.54	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001251.4	0.53	NRPS	epichloenin A	Epichloe festucae
BGC0002135.2	0.53	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000554.4	0.53	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0001250.4	0.52	NRPS	epichloenin A	Epichloe festucae
BGC0001758.4	0.52	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.51	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001641.4	0.51	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002174.2	0.77	NRPS	ε-Poly-L-lysine	Epichloe festucae
BGC0001135.5	0.60	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001251.4	0.60	NRPS	epichloenin A	Epichloe festucae
BGC0002135.2	0.60	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001250.4	0.60	NRPS	epichloenin A	Epichloe festucae
BGC0001758.4	0.60	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.59	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001641.4	0.59	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.59	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0000528.5	0.50	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for CG736_RS20880

allorf_118429_118530

Leader:

MSKVLELQKMVTVEGS

allorf_118429_118530

Leader:

MSKVLELQKMVTVEGS

Shared core:

PIDQEAISVSSCDSHSC

Core with dehydrations:

PIDQEAIDhaVDhaDhaCysDDhaHDhaCys

antiSMASH-DB 4.0 matches for allorf_118429_118530

100.0%

NZ_NNBO01000009.1and 3 others:

NZ_NNBO01000009.1 Lanthipeptides: allorf_118429_118530_lanthipeptideNZ_NNBO01000009.1 Lanthipeptides: allorf_118429_118530_lanthipeptideNZ_NNBO01000009.1 Lanthipeptides: allorf_118429_118530_lanthipeptide

Lanthipeptides: allorf_118429_118530_lanthipeptide

1

PIDQEAISVSSCDSHSC

17

PIDQEAISVSSCDSHSC

1

PIDQEAISVSSCDSHSC

17

72.2%

NZ_LISX01000002.1and 3 others:

NZ_LISX01000002.1 Lanthipeptides: allorf_740642_740746_lanthipeptideNZ_LISX01000002.1 Lanthipeptides: allorf_740642_740746_lanthipeptideNZ_LISX01000002.1 Lanthipeptides: allorf_740642_740746_lanthipeptide

Lanthipeptides: allorf_740642_740746_lanthipeptide

3

DQEAISVSSCDSHSC

17

DQEA+S+SSCDSHSC

4

DQEAVSLSSCDSHSC

18

70.6%

NZ_KE384306.1:

Lanthipeptides: allorf_0853037_0853141_lanthipeptide

4

QEAISVSSCDSHSC

17

QEAIS+SSCDS SC

1

QEAISISSCDSQSC

14

with 8 more sequence matches

55.6%

NZ_CP070496.1:

Lanthipeptides: JQS30_RS10335_lanthipeptide

6

AISVSSCDSHSC

17

AIS SSCDS SC

1

AISWSSCDSGSC

12

55.6%

NZ_LISX01000002.1and 3 others:

NZ_LISX01000002.1 Lanthipeptides: allorf_741046_741237_lanthipeptideNZ_LISX01000002.1 Lanthipeptides: allorf_741046_741237_lanthipeptideNZ_LISX01000002.1 Lanthipeptides: allorf_741046_741237_lanthipeptide

Lanthipeptides: allorf_741046_741237_lanthipeptide

4

QEAISVSSCDSHSC

17

+E+ S+SSCDSHSC

5

EESWSISSCDSHSC

18

41.2%

NZ_CP085710.1:

Lanthipeptides: allorf_3217696_3217818_lanthipeptide

7

ISVSSCDSHSC

17

+S +SCDS+SC

2

VSCTSCDSYSC

12

36.8%

NZ_CP085710.1:

Lanthipeptides: allorf_3218746_3218883_lanthipeptide

8

SVSSCDSHSC

17

S +SC SHSC

6

SCTSCTSHSC

15

36.8%

NZ_LISX01000002.1and 3 others:

NZ_LISX01000002.1 Lanthipeptides: allorf_740844_741005_lanthipeptideNZ_LISX01000002.1 Lanthipeptides: allorf_740844_741005_lanthipeptideNZ_LISX01000002.1 Lanthipeptides: allorf_740844_741005_lanthipeptide

Lanthipeptides: allorf_740844_741005_lanthipeptide

3

DQEAISVSSCDSHSC

17

DQE+ISVSSCDSHSC

24

DQESISVSSCDSHSC

38

26.1%

NZ_CP021121.1and 1 other:

NZ_CP084541.1 Lanthipeptides: LC193_RS06630_lanthipeptide

Lanthipeptides: CAG99_RS21195_lanthipeptide

3

DQEAISVSSCDSHS

16

D ++S+ CD+HS

2

DLSSLSLLICDTHS

15

25.9%

NZ_KQ948146.1:

Lanthipeptides: allorf_888774_889007_lanthipeptide

7

ISVSSCDSHSC

17

+S++SC SHSC

11

VSLTSCTSHSC

21

19.0%

NZ_CP029255.1and 1 other:

NZ_CP029255.1 Lanthipeptides: HYN56_RS19790_lanthipeptide

Lanthipeptides: HYN56_RS19790_lanthipeptide

4

QEAISVSSCDSHS

16

QEAIS++SC S

7

QEAISIASCKRGS

19

Lanthipeptide(s) for CG736_RS20895

allorf_118429_118530

Leader:

MSKVLELQKMVTVEGS

allorf_118429_118530

Leader:

MSKVLELQKMVTVEGS

Shared core:

PIDQEAISVSSCDSHSC

Core with dehydrations:

PIDQEAIDhaVDhaDhaCysDDhaHDhaCys

antiSMASH-DB 4.0 matches for allorf_118429_118530

100.0%

NZ_NNBO01000009.1and 3 others:

NZ_NNBO01000009.1 Lanthipeptides: allorf_118429_118530_lanthipeptideNZ_NNBO01000009.1 Lanthipeptides: allorf_118429_118530_lanthipeptideNZ_NNBO01000009.1 Lanthipeptides: allorf_118429_118530_lanthipeptide

Lanthipeptides: allorf_118429_118530_lanthipeptide

1

PIDQEAISVSSCDSHSC

17

PIDQEAISVSSCDSHSC

1

PIDQEAISVSSCDSHSC

17

72.2%

NZ_LISX01000002.1and 3 others:

NZ_LISX01000002.1 Lanthipeptides: allorf_740642_740746_lanthipeptideNZ_LISX01000002.1 Lanthipeptides: allorf_740642_740746_lanthipeptideNZ_LISX01000002.1 Lanthipeptides: allorf_740642_740746_lanthipeptide

Lanthipeptides: allorf_740642_740746_lanthipeptide

3

DQEAISVSSCDSHSC

17

DQEA+S+SSCDSHSC

4

DQEAVSLSSCDSHSC

18

70.6%

NZ_KE384306.1:

Lanthipeptides: allorf_0853037_0853141_lanthipeptide

4

QEAISVSSCDSHSC

17

QEAIS+SSCDS SC

1

QEAISISSCDSQSC

14

with 8 more sequence matches

55.6%

NZ_CP070496.1:

Lanthipeptides: JQS30_RS10335_lanthipeptide

6

AISVSSCDSHSC

17

AIS SSCDS SC

1

AISWSSCDSGSC

12

55.6%

NZ_LISX01000002.1and 3 others:

NZ_LISX01000002.1 Lanthipeptides: allorf_741046_741237_lanthipeptideNZ_LISX01000002.1 Lanthipeptides: allorf_741046_741237_lanthipeptideNZ_LISX01000002.1 Lanthipeptides: allorf_741046_741237_lanthipeptide

Lanthipeptides: allorf_741046_741237_lanthipeptide

4

QEAISVSSCDSHSC

17

+E+ S+SSCDSHSC

5

EESWSISSCDSHSC

18

41.2%

NZ_CP085710.1:

Lanthipeptides: allorf_3217696_3217818_lanthipeptide

7

ISVSSCDSHSC

17

+S +SCDS+SC

2

VSCTSCDSYSC

12

36.8%

NZ_CP085710.1:

Lanthipeptides: allorf_3218746_3218883_lanthipeptide

8

SVSSCDSHSC

17

S +SC SHSC

6

SCTSCTSHSC

15

36.8%

NZ_LISX01000002.1and 3 others:

NZ_LISX01000002.1 Lanthipeptides: allorf_740844_741005_lanthipeptideNZ_LISX01000002.1 Lanthipeptides: allorf_740844_741005_lanthipeptideNZ_LISX01000002.1 Lanthipeptides: allorf_740844_741005_lanthipeptide

Lanthipeptides: allorf_740844_741005_lanthipeptide

3

DQEAISVSSCDSHSC

17

DQE+ISVSSCDSHSC

24

DQESISVSSCDSHSC

38

26.1%

NZ_CP021121.1and 1 other:

NZ_CP084541.1 Lanthipeptides: LC193_RS06630_lanthipeptide

Lanthipeptides: CAG99_RS21195_lanthipeptide

3

DQEAISVSSCDSHS

16

D ++S+ CD+HS

2

DLSSLSLLICDTHS

15

25.9%

NZ_KQ948146.1:

Lanthipeptides: allorf_888774_889007_lanthipeptide

7

ISVSSCDSHSC

17

+S++SC SHSC

11

VSLTSCTSHSC

21

19.0%

NZ_CP029255.1and 1 other:

NZ_CP029255.1 Lanthipeptides: HYN56_RS19790_lanthipeptide

Lanthipeptides: HYN56_RS19790_lanthipeptide

4

QEAISVSSCDSHS

16

QEAIS++SC S

7

QEAISIASCKRGS

19

allorf_135626_135823

Leader:

MVDLRGLPAA

Core:

GARQALGRAVRAALRAAGRDRGRPAERLFTSSQDELPAETHVPLLGRCAETGRPQ

Core with dehydrations:

GARQALGRAVRAALRAAGRDRGRPAERLFDhbDhaDhaQDELPAEDhbHVPLLGRCysAEDhbGRPQ

antiSMASH-DB 4.0 matches for allorf_135626_135823

100.0%

NZ_NNBO01000009.1:

Lanthipeptides: allorf_135626_135823_lanthipeptide

1

GARQALGRAVRAALRAAGRDRGRPAERLFTSSQDELPAETHVPLLGRCAETGRPQ

55

GARQALGRAVRAALRAAGRDRGRPAERLFTSSQDELPAETHVPLLGRCAETGRPQ

1

GARQALGRAVRAALRAAGRDRGRPAERLFTSSQDELPAETHVPLLGRCAETGRPQ

55

16.4%

NZ_JAPTYM010000001.1:

Lanthipeptides: allorf_00301141_00301338_lanthipeptide

3

RQALGRAVRAALR

15

R ALG AVRA R

6

RSALGEAVRAGWR

18

16.4%

NZ_KN196470.1:

Lanthipeptides: LF63_RS03635_lanthipeptide

21

RGRPAERLFTSSQDELPA

38

+G PA LFT S +L A

1

KGNPAGSLFTVSNVDLKA

18

with 3 more sequence matches

14.5%

NZ_FOBB01000013.1:

Lanthipeptides: BMZ54_RS31540_lanthipeptide

41

HVPLLG-----RCAETGRP

54

++P+LG CA TG+P

13

NIPILGAISYAECASTGQP

31

12.7%

NZ_FNPT01000012.1:

Lanthipeptides: allorf_039443_039604_lanthipeptide

41

HVPLLGRCAE

50

H P +GRCAE

26

HSPGVGRCAE

35

12.7%

NZ_JACSPZ010000014.1:

Lanthipeptides: allorf_13953_14108_lanthipeptide

34

DELPAETHVPLL

45

D+LPA T PL

7

DDLPAITGCPLF

18

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (7):

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 20.92 out of 30.22

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 12.57 out of 26.06

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.40 out of 21.84

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.28 out of 26.33

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.21 out of 26.85

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.74 out of 29.38

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.39 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

Lanthipeptides

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Core peptide predictions

allorf_118429_118530 - Class II

Cleavage pHMM score: -2.40

RODEO score: 0

Monoisotopic mass: 1686.7 Da

Molecular weight: 1687.8 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 1705.8 Da N = 1; 1723.8 Da N = 2; 1741.8 Da N = 3

allorf_118429_118530 - Class II

Cleavage pHMM score: -2.40

RODEO score: 0

Monoisotopic mass: 1686.7 Da

Molecular weight: 1687.8 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 1705.8 Da N = 1; 1723.8 Da N = 2; 1741.8 Da N = 3

allorf_118429_118530 - Class II

Cleavage pHMM score: -2.40

RODEO score: 0

Monoisotopic mass: 1686.7 Da

Molecular weight: 1687.8 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 1705.8 Da N = 1; 1723.8 Da N = 2; 1741.8 Da N = 3

allorf_118429_118530 - Class II

Cleavage pHMM score: -2.40

RODEO score: 0

Monoisotopic mass: 1686.7 Da

Molecular weight: 1687.8 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 1705.8 Da N = 1; 1723.8 Da N = 2; 1741.8 Da N = 3

allorf_135626_135823 - Class II

Cleavage pHMM score: -4.80

RODEO score: 0

Monoisotopic mass: 5801.1 Da

Molecular weight: 5804.5 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 5822.5 Da N = 1; 5840.5 Da N = 2; 5858.5 Da N = 3; 5876.5 Da N = 4

Predicted core structure(s)

For candidate cluster 1, location 95944 - 136640:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 95944 - 129784:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

CG736_RS20860: X

AMP-binding (26..397): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FAELFDASCEELSTIFGGEWNTYGPTEATVVACG
Nearest Stachelhaus code(s):: Lys DAEDIGTVVK (71% 8Å match); Gln or Tyr DAESVGTVVK (65% 8Å match)
Stachelhaus code match:: 90% (moderate)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description

NZ_NNBO01000009.1 - Region 2 - terpene

Download region GenBank file

Download region SVG

Location: 223,746 - 244,705 nt. (total: 20,960 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: deacetylase

CG736_RS21445: acetylornithine deacetylase

smcogs: SMCOG1247: acetylornithine deacetylase

Lyases: cyclase

CG736_RS21425: Terpene synthase/cyclase metal-binding domain protein

smcogs: SMCOG1052: Terpene synthase/cyclase metal-binding domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002128.3	0.45	other (other)	5-dimethylallylindole-3-acetonitrile	Streptomyces coelicolor A3(2)
BGC0000559.4	0.37	ribosomal	subtilin	Bacillus subtilis subsp. spizizenii ATCC 6633
BGC0000043.5	0.37	PKS	curacin A	Moorea producens 19L
BGC0000506.4	0.37	ribosomal	entianin	Bacillus subtilis subsp. spizizenii
BGC0002316.2	0.36	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0002346.3	0.35	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0000511.5	0.34	ribosomal	ericin S	Bacillus subtilis
BGC0000527.5	0.34	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728
BGC0000310.5	0.33	NRPS	bacitracin	Bacillus licheniformis
BGC0000515.4	0.33	ribosomal	geobacillin I	Geobacillus thermodenitrificans NG80-2

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002128.3	0.45	other (other)	5-dimethylallylindole-3-acetonitrile	Streptomyces coelicolor A3(2)
BGC0000559.4	0.37	ribosomal	subtilin	Bacillus subtilis subsp. spizizenii ATCC 6633
BGC0000043.5	0.37	PKS	curacin A	Moorea producens 19L
BGC0000506.4	0.37	ribosomal	entianin	Bacillus subtilis subsp. spizizenii
BGC0002316.2	0.36	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0002346.3	0.35	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0000511.5	0.34	ribosomal	ericin S	Bacillus subtilis
BGC0000527.5	0.34	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728
BGC0000310.5	0.33	NRPS	bacitracin	Bacillus licheniformis
BGC0000515.4	0.33	ribosomal	geobacillin I	Geobacillus thermodenitrificans NG80-2

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

No product predictions

Genes with terpene domains:

CG736_RS21425: T1TS

Domain 1 (20 - 310): Type 1 terpene synthase

Subtype: unknown

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.80 out of 20.47

Confidence: medium

Additional weak hits (7):

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 19.29 out of 33.73

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 14.38 out of 21.55

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 18.21 out of 31.73

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 19.14 out of 22.40

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.31 out of 28.19

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 20.03 out of 35.63

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.81 out of 26.85

Confidence: weak

Terpene

TFBS Finder

Glossary

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TFBS definitions

Regulator	Description
VqsM	Regulator of quorum-sensing signalling systems

NZ_NNBO01000009.1 - Region 3 - redox-cofactor,triceptide

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Location: 319,658 - 341,796 nt. (total: 22,139 nt) Show pHMM detection rules used

Region on contig edge.

redox-cofactor: (cds(PF04055 and (TIGR04085 or SPASM)) and (TIGR02111 or TIGR03966) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]))
triceptide: ((cds(PF04055 and (TIGR04085 or SPASM)) and triceptide_precursor) or triceptide_rSAM)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

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Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: acyltransferase

CG736_RS21815: acyltransferase

smcogs: SMCOG1141: acyltransferase

Hydrolases: hydrolase

CG736_RS21865: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Ligases: synthase

CG736_RS21820: polyprenol-monophosphomannose synthase ppm1

smcogs: SMCOG1123: polyprenol-monophosphomannose synthase ppm1

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001100.5	0.17	NRPS, PKS	lankacidin C	Streptomyces rochei
BGC0000753.3	0.17	saccharide	capsular polysaccharide	Actinobacillus pleuropneumoniae
BGC0000806.5	0.12	saccharide	phosphonoglycans	Glycomyces sp. NRRL B-16210
BGC0000097.5	0.10	PKS	ML-449	Streptomyces sp. MP39-85
BGC0000029.5	0.10	PKS	BE-14106	Streptomyces sp. DSM 21069
BGC0001666.3	0.10	PKS	microansamycin A, microansamycin B, microansamycin C, microansamycin D, microansamycin E, microansamycin F, microansamycin G, microansamycin H, microansamycin I	Micromonospora sp. HK160111
BGC0002638.3	0.10	NRPS	misaugamycin A, misaugamycin B	Streptomyces sp. WAC 00631
BGC0000341.5	0.09	NRPS	enduracidin A, enduracidin B	Streptomyces fungicidicus
BGC0002657.2	0.09	NRPS, PKS	deoxyhangtaimycin	Streptomyces spectabilis
BGC0001036.5	0.09	NRPS, PKS	polyoxypeptin	Streptomyces sp. MK498-98F14

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001100.5	0.33	NRPS, PKS	lankacidin C	Streptomyces rochei
BGC0000753.3	0.32	saccharide	capsular polysaccharide	Actinobacillus pleuropneumoniae
BGC0000088.4	0.27	PKS	lovastatin	Aspergillus terreus
BGC0000235.5	0.26	PKS	kiamycin	Streptomyces sp. W007
BGC0000806.5	0.25	saccharide	phosphonoglycans	Glycomyces sp. NRRL B-16210
BGC0000097.5	0.23	PKS	ML-449	Streptomyces sp. MP39-85
BGC0000029.5	0.23	PKS	BE-14106	Streptomyces sp. DSM 21069
BGC0001666.3	0.22	PKS	microansamycin A, microansamycin B, microansamycin C, microansamycin D, microansamycin E, microansamycin F, microansamycin G, microansamycin H, microansamycin I	Micromonospora sp. HK160111
BGC0002638.3	0.22	NRPS	misaugamycin A, misaugamycin B	Streptomyces sp. WAC 00631
BGC0000341.5	0.21	NRPS	enduracidin A, enduracidin B	Streptomyces fungicidicus

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.39 out of 19.26

Confidence: strong

Additional weak hits (12):

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.57 out of 26.85

Confidence: weak

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.73 out of 19.26

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 16.24 out of 29.25

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.67 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.16 out of 27.78

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 18.38 out of 21.84

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.43 out of 20.97

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 18.76 out of 27.78

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.59 out of 22.40

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 13.59 out of 18.57

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.65 out of 28.19

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.04 out of 33.14

Confidence: weak

RREFinder

TFBS Finder

RRE predictions

: RRE type: PqqD_RRE; Location: 8 - 87; Score: 53.8
: RRE type: PqqD_RRE; Location: 8 - 87; Score: 53.8

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein