LOCUS       NZ_NNBO01000020        11279 bp    DNA     linear   CON 14-AUG-2024
DEFINITION  Kitasatospora sp. CB02891 scaffold20, whole genome shotgun sequence.
ACCESSION   NZ_NNBO01000020
VERSION     NZ_NNBO01000020.1
KEYWORDS    WGS; RefSeq.
SOURCE      Kitasatospora sp. CB02891
  ORGANISM  Kitasatospora sp. CB02891
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Kitasatospora.
REFERENCE   1  (bases 1 to 151376)
  AUTHORS   Pan,G., Xu,Z., Guo,Z., Ma,M., Yang,D., Zhou,H., Gansemans,Y.,
            Zhu,X., Zhao,L.-X., Jiang,Y., Cheng,J., van Nieuwerburgh,F.,
            Suh,J.W., Duan,Y. and Shen,B.
  TITLE     Discovery of the Leinamycin Family of Natural Products by Mining
            Actinobacterial Genomes
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 114 (2017) In press
REFERENCE   2  (bases 1 to 151376)
  AUTHORS   Pan,G., Xu,Z. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUL-2017) Chemistry, The Scripps Research Institute,
            130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 04-APR-2016
            Assembly Method                   :: CLC NGS Cell v. 7.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002803145.1-RS_2024_08_14
            Annotation Date                   :: 08/14/2024 03:20:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,223
            CDSs (total)                      :: 7,128
            Genes (coding)                    :: 7,004
            CDSs (with protein)               :: 7,004
            Genes (RNA)                       :: 95
            rRNAs                             :: 8, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 32 of 124
            Pseudo Genes (incomplete)         :: 102 of 124
            Pseudo Genes (internal stop)      :: 10 of 124
            Pseudo Genes (multiple problems)  :: 18 of 124
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:31:40
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 71068
            Orig. end                         :: 82347
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NNBO01000020.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11279
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11279
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11279
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6279](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6279)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            <255..1688
                     /gene="hemG"
                     /locus_tag="CG736_RS32305"
                     /old_locus_tag="CG736_32215"
                     /pseudo=""
     CDS             <255..1688
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138585.1"
                     /locus_tag="CG736_RS32305"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="CG736_32215"
                     /product="protoporphyrinogen oxidase"
                     /pseudo=""
                     /sec_met_domain="DAO (E-value: 3.2e-13, bitscore: 42.8,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="GFGIRAGRLRDMAKPHVVVIGGGVAGLAAAAFLSDRPVRVTVLEA
                     SDRFGGKLRGGEVGGVRVDLGAESMLARRPEAVELARQVGLGEQLEPPTTAKASIWTRE
                     GLRPMPGGQLMGVPGDLDALKASGVLTPEGLERAVHEQPAEIGDDAPIGAYIAARLGRE
                     VVDRLVEPLLGGVYAGHADEISIRAAVPQLLALAQRDGSLTEAVRELTGRSTATGPVFQ
                     GLRGGLGTFPDAIAAHDRAHGAELLTGHPVEALRRTPDGWQVTTGGRQLHADAVVLAVP
                     APHAAALLAADAPAAAAELRGIEYASMALVTLAFRRADLADSPLVGSGFLVPPVDRRTI
                     KASTFSSNKWGWLECSAPDTFLLRTSLGRHREAAALQLDDAELVARSLADLQTAVGLRA
                     TPYDHTVTRWTDGLPQYPVGHLDRVARIRTRLDALGGIAVCGAAYDGVGIPAVVASARR
                     AAAVHPVTGTTRTEGTMGS"
     gene            1685..2395
                     /gene="hemQ"
                     /locus_tag="CG736_RS32310"
                     /old_locus_tag="CG736_32220"
     CDS             1685..2395
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138586.1"
                     /locus_tag="CG736_RS32310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CG736_32220"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_043909976.1"
                     /transl_table=11
                     /translation="MTESTEQPVKKKARDLNQVIRYTMWSVFKLKDGLPEDRSALVAEV
                     DALFEQLAAKDVTIRGTYDVSGLRADADLMIWWHAENSDDLQEAYNRFRRTELGRRMEP
                     VWSNMALHRPAEFNKSHIPAFLADERAREYVCVYPFVRSYEWYLLPDEERRAMLSEHGQ
                     MARGYPDVRANTVASFALGDYEWLLAFEADELHRIVDLMRDLRPSRARLHVREEVPFFT
                     GRRKSVGDLVAGLF"
     gene            complement(2473..2940)
                     /locus_tag="CG736_RS32315"
                     /old_locus_tag="CG736_32225"
     CDS             complement(2473..2940)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CG736_RS32315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="CG736_32225"
                     /product="hypothetical protein"
                     /protein_id="WP_100589679.1"
                     /transl_table=11
                     /translation="MIRFRIAAAAVLVLSPVVLSAPGPAALAVEQHRAPSVSQPQAFRT
                     PAEQRVDTFFSAYRDAVLQGGPEAARTVRQKYLTADLNARLDRWAQANDADPVFRAQDV
                     PVGWTVAQDSSGAGHTAVLLTEQWGDGSTTPVEYQLRLPDLEIDDLENAPA"
     gene            complement(3033..3794)
                     /locus_tag="CG736_RS32320"
                     /old_locus_tag="CG736_32230"
     CDS             complement(3033..3794)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019073953.1"
                     /locus_tag="CG736_RS32320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CG736_32230"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_100589680.1"
                     /transl_table=11
                     /translation="MWVLLLIPTCAAAVLSCLRLVRVASVADALVEHLPTADRTSGIGL
                     YETAYLAGGPARVVDLALVRMAGLGRLHLAHTGWTTVVDPQGRGRLERALITAIGPEGQ
                     CRTAQLKAALAAHPTVRDIGDRLALAGLATPAEVRVRALRAIRHVRYALLGSVAMLLLT
                     VVLNSDGELARTAVAWYSLPLVLTGGTLMMARVDVHSYTAWAAPAGQRLLRTLRWPHIC
                     RAGTDDLLTAVAIRGPEAVQDPRLRAALSHR"
     gene            complement(3875..4918)
                     /locus_tag="CG736_RS32325"
                     /old_locus_tag="CG736_32235"
     CDS             complement(3875..4918)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="CG736_RS32325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CG736_32235"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_100589681.1"
                     /transl_table=11
                     /translation="MWNTQFVVALVMVALSMVVATVFDRRCRRVTEPRGLPGNGMPLLE
                     AAFLAGGPARAVDTALVRMEREGRVVISRAQRVTVTDTAPRDAVETVLITAVGGRTGRD
                     LSVLRASVLGSPDVQRIGDALAARGLLRRPAALKAAWRARRLVVLALVVTLVLGTAATV
                     QWLSEPGRDAATPPFFAFGALAVVAVVHLVVGRPPRARITPAGERQLQLMHGSSPWRPR
                     EVDRGHAALVGAFALDGPAALKDAADRPLREALGGTAPRPASLVKAGRSSSSGGAAETG
                     GAAGAAWCGTTVSSCGSSGHSGHSGGHSGHSGGHSCGGSSHSCGSSSSCGSSSSCGSSC
                     GGGGCGS"
     gene            complement(5001..6279)
                     /locus_tag="CG736_RS32335"
                     /old_locus_tag="CG736_32245"
                     /pseudo=""
     CDS             complement(5001..6279)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944755.1"
                     /locus_tag="CG736_RS32335"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="CG736_32245"
                     /product="DUF692 domain-containing protein"
                     /pseudo=""
                     /sec_met_domain="DUF692 (E-value: 1.7e-88, bitscore: 289.2,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSHGIPRLGTGLGWRPEIDTAVERLPGLDWVEVVAENVCADHLP
                     PSLTALRGRGVTVVPHGVSLGLGDAAAPDPARLARLAAVAGALGSPLVSEHLAFVRAGG
                     LEAGHLLPVPRTREALRVVAANVRIAQQELPVPLALENIAAHLAWPGDELTEAQFLAEL
                     VERTGVHLLVDVANLHTNRVNLGVDPAAELDRLPLDALAYVHVAGGVERDGVWHDTHTH
                     PVTAPVLEVLAALCERTDPPGVLLERDGAYPPPAELSAELDAVRRVHRTAHDRRKEPDR
                     VRTGRVPDP"
     gene            6472..7386
                     /locus_tag="CG736_RS32340"
                     /old_locus_tag="CG736_32250"
     CDS             6472..7386
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015607601.1"
                     /locus_tag="CG736_RS32340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CG736_32250"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_232734330.1"
                     /transl_table=11
                     /translation="MPMTSMPYDIRPFAPEDAAGATDAYRAGRPHLVVTPEVTSWLVGL
                     PHYHLLVAERAGQVVGSARFGPVTESTVPHQGFLNVSVLPEHRGSGIGTALLTAAEREL
                     AVLGVRDAHGWADGTPEALGFAAARGYRRGRRAQFAGRDLTVPLPPLPPTPPGIELRTA
                     ADFLDDPYPVYLVDIDGTRAEPGDLDLADQSYESWLAEIWQRPDQDHTLTTVVLDRGRA
                     VAFSAAQTDGRSYWSAFTATCSTHRGRGLAKLAKAHSLHRAQARGLTAAYTNNDGDNAP
                     MLAINDWFGYRVCAEEWKHSRTL"
     gene            complement(7408..9537)
                     /locus_tag="CG736_RS32345"
                     /old_locus_tag="CG736_32255"
     CDS             complement(7408..9537)
                     /EC_number="3.1.21.-"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1296: tellurium
                     resistance protein, TerD"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF014404.4"
                     /locus_tag="CG736_RS32345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CG736_32255"
                     /product="restriction endonuclease"
                     /protein_id="WP_157815822.1"
                     /transl_table=11
                     /translation="MFGLDVGGAGVARRRSSGVWSVLAEAQREQHRRVEAQRKAAAAQQ
                     RDHERAQREAQRAAARGEREALKAYQQQRDADAARRTAELDDRVAELRGVLAAGLAGPG
                     FSLAEQSRGGQGAIPPFDPGPLGVPVPMPDQNWYLVPPLTGAQAYNPAARRQWEEQAGH
                     ARARFEYDWQAAWAAEQQRQRQLADYRAQYDAWAAERHRLLAGQSTQAGRLAQRLRAGE
                     AAAVAEYFEAVVDWREDWPDGFPTDGEASWDADTRRLVVRWELPAFDVVPTVGRYRYVR
                     SDDREDEVARPAGQRKEIYREVLAQCALRVLAEVFRADPDGLIATVGLNGVVVAPDPAT
                     GQEGDRCLLAVEVHRATFAGLALDRVAPLDCLQDALGGRLSARPEKADTVAEIPTAATS
                     AGDGEEPDLFAMDPIEFEKLIAELFRRRGFRTSTTARSGDEGVDVLAEDPDPITGGKIV
                     IQAKRYRHTVSPSAVRDLESTMRRQGANRGILVTTSGFGPGSRKHAEGQPLTLVDGPML
                     LTLLREHGLPGRLGPAPVPARQADGPAAVELMPGQNTVLPDGEVRVRFRSDGADADLTL
                     LLLDALGKVRADEDFVFYHQPTAAAGAVTLEPGDGSAVVRTDRLPDTVHRVAVSVNLDA
                     DGDATCADLIDPTVELAAGPGRWTFRPPTDPAISAMLVAELYRHPADGWKLRAVGQGWS
                     DGLAGLARHHGVDVE"
     gene            complement(9577..9981)
                     /gene="msrB"
                     /locus_tag="CG736_RS32350"
                     /old_locus_tag="CG736_32260"
     CDS             complement(9577..9981)
                     /EC_number="1.8.4.12"
                     /GO_function="GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /GO_process="GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /GO_process="GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /gene="msrB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138594.1"
                     /locus_tag="CG736_RS32350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CG736_32260"
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="WP_100589684.1"
                     /transl_table=11
                     /translation="MSYEVQKSDAEWRSELSPEEYHVLREAGTERPFVGEYTDTRTVGV
                     YSCRACGAELFSSETKFESHCGWPSYYAPLAGDSVEYLEDSSLGMRRVEVRCKKCGSHL
                     GHVFEGEGYDTPTDQRYCINSISLRLKPAE"
     misc_feature    complement(10232..10250)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 19.03"
                     /tool="antismash"
     misc_feature    complement(11237..11251)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.25"
                     /tool="antismash"
ORIGIN
        1 ccgcggatgt gctggtgccg ctgccgttcg cgctgcaccc ggccagcagc agcaccgccg
       61 ccaggacgcc ggccgccccc gctctgcgca cccgtactcc catgcccgtc ccctccccgt
      121 gtccgtgcgg cgggccgctc gcccgccctc aagcactgtg acgcgcgggc ccgcggaccg
      181 gacccgcgcc ggcggtaccg gaacgatcac gcctcggcct cacggcggtc tcgacggtcg
      241 cgtgggccga ttccggattc gggataaggg ctggaagact gcgggacatg gcgaaacctc
      301 acgtcgtggt gatcggtggc ggcgtcgcgg gcctggccgc ggccgccttc ctcagtgacc
      361 ggccggtgcg ggtcaccgtg ctggaggcgt ccgaccgctt cggcggcaaa ctgcgcggcg
      421 gggaggtcgg cggcgtccgg gtcgatctcg gcgcggagtc gatgctggcc cgccgcccgg
      481 aagccgtcga actggcccgg caggtcggtc tcggcgagca gttggagccg ccgaccaccg
      541 ccaaggcgtc gatctggacc cgggagggcc tgcgcccgat gcccggcggc cagttgatgg
      601 gcgtgcccgg cgacctggac gcgctgaagg cctccggcgt gctcacgccc gaaggcctgg
      661 agcgggccgt ccacgagcag cccgccgaga tcggcgacga cgccccgatc ggcgcctaca
      721 tcgccgcccg gctcggccgc gaggtcgtcg accggctggt cgagccgctg ctcggcggcg
      781 tgtatgccgg gcacgccgac gagatctcga tccgcgccgc cgtcccgcag ctgctggcac
      841 tggcgcagcg cgacggctcg ctgaccgagg cggtgcgcga gctcaccggc cgctcgaccg
      901 ccaccggccc ggtcttccag ggcctgcgcg gcggcctcgg caccttcccc gacgccatcg
      961 ccgcgcacga ccgcgcgcac ggcgccgaac tgctcaccgg ccaccccgtc gaggcgctgc
     1021 gccgcacccc cgacggctgg caggtcacca ccggcggacg gcaactgcac gcggacgccg
     1081 tggtgctggc cgtccccgcc ccgcacgccg ccgcgctgct cgccgccgac gcgcccgccg
     1141 ccgcggccga gctgcggggg atcgagtacg cctcgatggc gctggtcacg ctcgccttcc
     1201 ggcgcgccga cctggcggac agcccgctcg tcggcagcgg cttcctggtg ccgccggtgg
     1261 accgccgcac catcaaggcg tccaccttct ccagcaacaa gtggggctgg ctggaatgct
     1321 ccgcgccgga caccttcctg ctgcgcacct cgctgggccg gcaccgcgag gccgccgcgc
     1381 tccagctgga cgacgccgaa ctggtggccc gttcgctggc cgacctgcag accgccgtcg
     1441 gcctgcgcgc caccccgtac gaccacaccg tcacccgctg gaccgacggt ctgccgcagt
     1501 acccggtcgg ccacctcgac cgggtcgccc ggatccgcac ccggctggac gcgctcggcg
     1561 gcatcgccgt ctgcggcgcc gcctacgacg gcgtcggcat ccccgccgtc gtcgcgagcg
     1621 cccgccgggc cgccgccgtc caccccgtca ccggaaccac ccgaacagaa gggacaatgg
     1681 gctcatgacc gaaagcactg agcagcctgt gaagaagaag gcccgcgacc tcaaccaggt
     1741 catccgctac accatgtggt cggtgttcaa gctgaaggac ggcctccccg aggaccgttc
     1801 cgccctggtc gccgaagtgg acgccctctt cgagcagctc gccgccaagg acgtcaccat
     1861 ccgcggcacc tacgacgtgt ccggcctgcg cgccgatgcc gatctgatga tctggtggca
     1921 cgcggagaac tcggacgacc tgcaggaggc gtacaaccgc ttccgccgca ccgagctggg
     1981 ccgccggatg gagccggtct ggtcgaacat ggcgctgcac cgccccgccg agttcaacaa
     2041 gtcgcacatc cccgcgttcc tcgccgacga gcgggcccgc gagtacgtct gcgtctaccc
     2101 gttcgtccgc tcctacgagt ggtacctgct ccccgacgag gagcgccggg cgatgctgtc
     2161 cgagcacggc cagatggccc gcggctaccc ggacgtgcgc gccaacaccg tcgcctcgtt
     2221 cgcgctcggc gactacgagt ggctgctggc cttcgaggcc gacgagctgc accgcatcgt
     2281 cgacctgatg cgcgacctgc gcccgtcccg cgcgcggctg cacgtgcgcg aggaggtgcc
     2341 gttcttcacc ggccgccgca agtcggtcgg cgacctggtc gccgggctgt tctgacccga
     2401 ccgctccgaa ccgctccgac agccgtcacc gcgcccccgc gcggcggcgg ctgccggcgt
     2461 tccggggcgc ggtcacgcgg gggcgttctc caggtcgtcg atctccaggt cgggcagtcg
     2521 cagctggtac tcgacggggg tggtggagcc gtcgccccac tgctcggtga gcagcacggc
     2581 ggtgtgtccg gcgccgctgc tgtcctgggc gacggtccag ccgaccggga cgtcctgcgc
     2641 ccggaacacc gggtcggcgt cgttggcctg cgcccaccgg tcgagccggg cgttcaggtc
     2701 ggcggtcagg tacttctgcc ggaccgtcct ggccgcctcg ggcccgccct ggaggacggc
     2761 gtcgcggtac gcggagaaga acgtgtcgac ccgctgctcg gcgggcgtcc ggaacgcctg
     2821 cggctgcgag acgctcgggg cccggtgctg ttcgacggcg agtgccgccg ggccgggcgc
     2881 cgacaggacg accggcgaga ggacgaggac cgccgcggcg gcgatgcgga aacgaatcat
     2941 gccgtcgagt ctgcgagcgg ccggagggcc ccggcgcaca acgcgctgac gcggcgtcgg
     3001 aactcggccc ggacggcccc gggcccggcc ggctaacggt ggctcagcgc ggcccgcagg
     3061 cgcgggtcct ggacggcctc gggcccgcgg atcgcgaccg cggtgagcag gtcgtcggtg
     3121 cccgcccggc agatgtgcgg ccagcgcagg gtgcgcagca ggcgctgccc ggcgggggcc
     3181 gcccaggcgg tgtaggagtg cacgtcgacc cgggccatca tcagggtgcc gccggtcagc
     3241 accagcggca gcgagtacca ggcgacggcc gtccgggcca gttcgccgtc ggagttcagc
     3301 accaccgtga gcagcagcat cgcgaccgac ccgagcagtg cgtaccggac gtgccggatc
     3361 gcccgcagcg cccggacccg gacctccgcc ggcgtggcca gaccggccag cgccagccgg
     3421 tcgccgatgt cccggacggt ggggtgggcg gccagcgccg ccttcaactg ggcggtgcgg
     3481 cactggccct cggggccgat cgcggtgatc agggcccgct ccagccggcc ccggccctgc
     3541 ggatcgacca ccgtggtcca cccggtgtgc gccaggtgca gccggcccag cccggccatc
     3601 cggaccagcg ccagatccac cacccgggcc ggtccgcccg ccaggtacgc ggtctcgtac
     3661 aggccgatcc ccgaggtccg gtcggccgtc ggcaggtgct cgacgagggc gtcggcgacc
     3721 gaggcgaccc ggaccaggcg cagacaggac agcaccgcgg cggcacaggt cgggatcagc
     3781 agcagcaccc acatggtgtt tgttctagtc ccgctcacgc ggcaagcgga atggatccac
     3841 ctgccgcgat accgaactgt gacacgatcc cgactcagga cccgcagccc ccgccgccgc
     3901 agctcgaccc gcacgaactg gacgaaccgc acgaactcga cgacccgcag gagtgtgagg
     3961 acccgccgca ggagtgcccg cccgagtgcc ccgagtgccc gccggagtgc cccgagtgtc
     4021 cggacgagcc gcaggagctg acggtggttc cgcaccacgc cgcgcccgcg gcccctccgg
     4081 tctccgccgc gccgcccgag ctgctcgaac gccccgcctt caccagggag gccggccggg
     4141 gcgcggtgcc gccgagcgcc tcgcgcagcg gccggtccgc ggcgtccttc agcgcggcgg
     4201 gcccgtccag ggcgaacgcg ccgaccaggg cggcgtgccc gcggtccacc tcgcgcggac
     4261 gccacgggct gctgccgtgc atcagctgca gctgacgctc cccggcgggc gtgatccgcg
     4321 ccctcggcgg ccggccgacc accaggtgca cgacggcgac cacggccagc gccccgaacg
     4381 cgaagaacgg gggagtggcg gcgtcccgcc ccggctcgga cagccactgg acggtggcgg
     4441 ccgtgcccag caccagggtg accaccagcg cgagcaccac cagccggcgg gcccgccagg
     4501 cggccttgag cgcggcgggc cggcgcagca ggccgcgggc ggccagcgcg tcaccgatcc
     4561 gctgcacgtc cgggctgccc agcacggagg cgcgcagcac ggacaggtcc cgcccggtgc
     4621 ggccgccgac ggcggtgatc agcacggtct cgacggcgtc gcgcggcgcg gtgtcggtga
     4681 ccgtgacccg ctgggcccgg gagatcacca cccggccctc gcgttccatc cggaccagcg
     4741 cggtgtccac cgcccgggcc gggccgccgg ccaggaacgc ggcctccagc agcggcatgc
     4801 cgttcccggg caggccccgg ggctcggtga cccggcggca gcgccggtcg aagacggtgg
     4861 cgacgaccat ggacagggcg accatgacca gggccacgac gaactgggtg ttccacattc
     4921 ccccgaagtc ctttcgtgca gtggtgcgag aggcggcgac acggccggtg aggtggtgcc
     4981 gagtgcggcg ttcgcgaggg ctaccggtgc gcccgggcgg tgcggcgcgg cagcagaccg
     5041 gcccagcggc gggcggccgg gtggagcaga cggtgcagcg ccgggtgcca cggcgcggcg
     5101 ggtgcggcgt ccagggccca ggcggcgaag gcgtgggcgt cggcccggta gccgccggtg
     5161 agcgggcggc cgacggcgta gcggcggaac agcggtgcga agtccgggcc gagcagctcg
     5221 ggcagttcgg gcatcaggtg gccgacggtc tcccggcgct tggcgagcag gccctcggac
     5281 tgggtgtgca ggtgcgtcgg gtcgaacccg gcgggggcgg ggccgcccgc caccagggcg
     5341 gtcagcagct cctcctgacg ggcggccagg ttgcggcggg ccagggcgag ttcggcgtcg
     5401 gcgcggtcac ggatcaggta ctcgtccagt tcggacacgg tcgggctcct tccggcggtc
     5461 gtgggccgtg cgatggaccc ggcgaacggc gtcgagttcg gcggacagtt cggcgggcgg
     5521 cgggtaggcg ccgtcccgct ccagcagcac cccgggcggg tcggtgcgtt cgcacagtgc
     5581 ggcgagcacc tccagcacgg gggcggtgac gggatgggtg tgggtgtcgt gccagacgcc
     5641 gtcgcgctcc accccgccgg cgacgtgcac gtaggccagc gcgtccagcg gcagccggtc
     5701 gagctcggcg gccgggtcga cgcccaggtt gacccggttg gtgtgcaggt tggcgacgtc
     5761 caccagcagg tgcacgccgg tgcgctcgac gagctcggcg aggaactggg cctccgtcag
     5821 ctcgtcgccc ggccaggcca ggtgcgcggc gatgttctcc agcgccaacg gcaccggcag
     5881 ttcctgctgg gcgatccgca cgttcgcggc caccacccgc aacgcctccc gggtgcgcgg
     5941 caccggcagc aggtggccgg cctccaggcc gcccgcccgg acgaacgcca gatgctcgct
     6001 gaccagcggc gaaccgagcg ctccggcgac cgcggccagc cgcgccagcc gcgccggatc
     6061 gggcgcggcg gcatcgccga ggcccagcga gacgccgtgc ggaacgaccg tcacgccccg
     6121 gccccgcagc gccgtcaacg agggcggcag gtggtcggcg cagacgttct cggcgaccac
     6181 ctccacccag tccagcccgg gcagccgctc cacggcggtg tcgatctcgg ggcgccagcc
     6241 gagccccgtg cccaaccgcg gaattccgtg cgaggacata ttccacccct tcccgccggg
     6301 cctttcgacg gcctcgactc cccctgccga gggtgtatcc ggccggggcc ggtgccaagc
     6361 gggacaccgg aaactcagag ctgaacttga gctttccgtc ccgctgaacg ggacggcccg
     6421 caggtggggg gaaattcagc ggcgcgcagg cgaacgactg actaacgtga catgcctatg
     6481 acttcgatgc cgtacgacat ccgcccgttc gcccccgagg acgccgccgg ggccaccgac
     6541 gcctaccggg ccggccgacc gcacctggtc gtcacacccg aggtgaccag ctggctcgtc
     6601 ggcctgccgc actaccacct gctggtcgcc gaacgcgccg gacaggtggt cggctccgcg
     6661 aggttcggcc cggtcaccga gagcaccgtc ccgcaccagg gcttcctgaa cgtcagcgtg
     6721 ctgcccgagc accgcggcag cggcatcggc accgcgctgc tcaccgccgc cgaacgcgaa
     6781 ctcgccgtcc tcggcgtccg cgacgcccac ggctgggcgg acggcacccc cgaagccctc
     6841 ggcttcgccg ccgcccgcgg ctaccggcgg ggccgccgcg cccagttcgc cggccgcgac
     6901 ctcaccgtcc cgctgccgcc actgccgccc accccgcccg gcatcgaact ccgcaccgcc
     6961 gcagacttcc tggacgaccc gtaccccgtc tacctggtcg acatcgacgg cacccgcgcc
     7021 gaacccggcg acctcgacct ggcggaccag tcctacgaga gctggctcgc cgagatctgg
     7081 cagcggcccg accaggacca caccctcacc accgtcgtcc tcgaccgggg ccgggcggtc
     7141 gccttctccg cggcccagac cgacggccgc agctactggt ccgcgttcac cgcgacctgc
     7201 tccacccacc gcggccgcgg cctcgccaaa ctcgccaagg cccactccct gcaccgcgcg
     7261 caggcccgcg gcctgaccgc ggcgtacacc aacaacgacg gcgacaacgc gccgatgctc
     7321 gccatcaacg actggttcgg gtaccgggtg tgcgcggagg agtggaagca cagccgcacg
     7381 ctctgactcc ggggacgccc tcgcccgcta ctcgacgtcg acgccgtggt ggcgggccag
     7441 gcccgccagg ccgtcggacc agccctggcc cacggcacgg agtttccagc cgtcggcggg
     7501 gtggcggtag agctcggcga cgagcatcgc ggagatggcc gggtcggtcg gcgggcggaa
     7561 ggtccagcgg ccggggccgg cggcgagttc caccgtcggg tcgatcaggt ccgcgcaggt
     7621 ggcgtcgccg tcggcgtcca ggttcacgga cacggccacg cggtggacgg tgtccggcag
     7681 gcggtcggtg cggaccaccg cgctgccgtc gccgggttcc agggtgacgg ccccggcggc
     7741 cgcggtcggc tggtggtaga agacgaagtc ctcgtcggcc cggaccttgc cgagcgcgtc
     7801 gagcagcagc agcgtcaggt cggcgtccgc gccgtcggac cggaagcgga cccgtacctc
     7861 gccgtcgggc agcacggtgt tctgaccggg catcaactcc acggccgcag gcccgtcggc
     7921 ctgccgggcg gggaccgggg ccgggcccag tcggccgggg aggccgtgct cgcggagcag
     7981 ggtgagcagc atcgggccgt ccaccagggt gagcggctgg ccctcggcgt gcttgcgcga
     8041 gccggggccg aagcccgaag tggtcaccag gatgccgcgg ttggcgccct ggcggcgcat
     8101 cgtggactcc aggtcgcgga cggcgctggg ggagacggtg tgccggtagc gcttggcctg
     8161 gatgacgatc tttccgccgg tgatcgggtc ggggtcctcg gcgagcacgt ccacgccctc
     8221 gtcgccgctg cgggcggtgg tgctggtgcg gaagccgcgg cggcggaaca gctcggcgat
     8281 cagcttctcg aactcgatcg ggtccatcgc gaacaggtcc ggctcctcgc cgtccccggc
     8341 ggaggtcgcg gcggtcggga tctcggccac cgtgtcggcc ttctcgggcc gggcggagag
     8401 ccgtccgccg agcgcgtcct gcaggcagtc cagtggtgcg acgcggtcca gggccagccc
     8461 ggcgaaggtc gcccggtgca cctccaccgc cagcaggcag cggtcgccct cctggccggt
     8521 cgccgggtcc ggggccacca cgacgccgtt caggccgacg gtggcgatca gcccgtcggg
     8581 gtcggcccgg aacacctccg ccaggacccg cagcgcgcac tgcgccagca cctcgcggta
     8641 gatctccttg cgctggccgg ccggccgggc cacctcgtcc tcccggtcgt cggaccggac
     8701 gtagcggtag cggccgaccg tcggcaccac gtcgaacgcg ggcagttccc agcgcaccac
     8761 cagccggcgg gtgtccgcgt cccaggaggc ctcgccgtcg gtggggaagc cgtccggcca
     8821 gtcctcccgc cagtcgacga ccgcctcgaa gtactcggcg accgccgccg cctcgcccgc
     8881 ccgcagccgc tgcgcgagcc gccccgcctg ggtgctctgc ccggccagca gacggtgccg
     8941 ctcggccgcc cacgcgtcgt actgggcgcg gtagtcggcg agttggcgct gccgctgctg
     9001 ctcggccgcc caggccgcct gccagtcgta ctcgaacctg gcccgggcgt gcccggcctg
     9061 ttcctcccac tggcggcgcg ccgccgggtt gtacgcctga gcgcccgtca acggcggcac
     9121 caggtaccag ttctgatcgg gcatcggcac cggaacgccc agcggccccg ggtcgaacgg
     9181 cgggatcgcg ccctgcccgc cccggctctg ctccgccagc gagaaccccg gaccggccag
     9241 ccccgccgcc agcaccccgc gcaactcggc gacccggtcg tccagttcgg ccgtccgacg
     9301 ggccgcgtcc gcgtcccgct gctgctggta cgccttcagc gcctcgcgct cgccccgcgc
     9361 cgcggctcgc tgcgcctcgc gctgcgcccg ctcgtggtcc cgctgttggg cggccgcggc
     9421 cttccgctgc gcctcgacgc ggcggtgctg ctcacgctgc gcctccgcca gtaccgacca
     9481 caccccggaa gaccgccgcc gcgccacgcc cgccccaccc acgtccagac cgaacaagag
     9541 cgatcagtct tccacggggg cgggcgtcgg cgcttcctac tcggcgggct tcagccgcag
     9601 cgagatcgag ttgatgcagt accgctggtc cgtcggcgtg tcgtagccct cgccctcgaa
     9661 cacgtgcccg aggtggctgc cgcacttctt gcagcgcacc tcgacccggc gcatcccgag
     9721 gctggagtcc tccaggtact ccaccgagtc accggccagc ggggcgtagt aggacggcca
     9781 gccgcagtgc gactcgaact tcgtctcgct gctgaacagc tccgcgccgc acgcccggca
     9841 gctgtacacg ccgacggtcc tggtgtcggt gtactcgccg acgaacgggc gctcggtgcc
     9901 ggcctcgcgg agcacgtggt actcctcagg gctcagctcg gaccgccact cggcgtcgga
     9961 cttctggacc tcgtagctca cggcactctc ccgggctcgc cgttcgactc agacagcagg
    10021 tgcaacgtcc gccagggcgg ccagaatttc cgggcccaga ttggtgacgt cgcccgcgcc
    10081 catggtcagc accaggtcgc cggggcgggc caggccggcc agcacggcgg gcgcggcggc
    10141 gaactcgtgc tcggcccgga cgtccgcgcc ggcccggcgg gccgcgtcga tgatcagctc
    10201 ggcggtgacg cccgggatcg ggtcctcacg ggccgggtag atgtccagca cgaccgagcc
    10261 gtcggccagc gccagcgccc ggcccatctc ctcggccagc tgctgggtgc gggagaacag
    10321 gtgcggctgg aacaccacca gcacccggcc cgcgcccgcc gcctcgcgga tcgcctccag
    10381 gtcggcggcc atctcggtcg ggtggtgcgc gtacgagtcg atcacctgca cgccgcgggc
    10441 ctcgcccttc agctgcagcc gccgccgcac gccggtgtac gcgcccagcg ccttcgccag
    10501 cccgtccgcc ggaacgccca gcgcgacgcc cgcggccagc gccgccaccg cgttgtgcgc
    10561 gtagtgccgg cccggcaccg agacggtgaa cgtcagccgc tcgccgccca gttcgaccgt
    10621 cacctcgctg gtcatgccgt gcgccgccac cgacagcacc cgcacgtccg cgtcctcggc
    10681 cgcgccgacc gtcaccacgt tcaggcccgc ccgcccggcg acccggcggg tcagctcgcg
    10741 ggcgccctcg tggtcggccg acaccaccag ggtgccgccg gggacgatcc gggcgacgaa
    10801 cgtctcgaag gactcgtaga tctcctccat cgacgcgtag ttggcgtggt ggtccagctc
    10861 cacgttcagc acgatcgcca cctcgggcgc gtacttgtgg aagctgcggt cgctctcgtc
    10921 cgcctccgcg acgaagatgt cgcccgcccc gtggtgcgcg ttcgacccgg gcaggtccag
    10981 gtcgccgccg atcgcgtacg agggctccag gcccagctcg cccaggctga ccgccagcat
    11041 cgaggtggtg gtggtcttcc cgtgggtgcc cgccaccgcg agcgcccgcc ggccgcccat
    11101 cagcgccgcc agcgcgtccg aacggtgcac caccgggacg ccccgctcct gcgccgccac
    11161 cagctccggg ttgtcggcgc ggatcgcgct ggagaccacc acgctgctcg tcccggccgg
    11221 caggtgctcg gccgcgtggc cgacgaacac cgtcgcgccg agctcgcgca gcgcccgca
//
