LOCUS       NZ_NNBO01000006        10801 bp    DNA     linear   CON 14-AUG-2024
DEFINITION  Kitasatospora sp. CB02891 scaffold6, whole genome shotgun sequence.
ACCESSION   NZ_NNBO01000006
VERSION     NZ_NNBO01000006.1
KEYWORDS    WGS; RefSeq.
SOURCE      Kitasatospora sp. CB02891
  ORGANISM  Kitasatospora sp. CB02891
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Kitasatospora.
REFERENCE   1  (bases 1 to 409919)
  AUTHORS   Pan,G., Xu,Z., Guo,Z., Ma,M., Yang,D., Zhou,H., Gansemans,Y.,
            Zhu,X., Zhao,L.-X., Jiang,Y., Cheng,J., van Nieuwerburgh,F.,
            Suh,J.W., Duan,Y. and Shen,B.
  TITLE     Discovery of the Leinamycin Family of Natural Products by Mining
            Actinobacterial Genomes
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 114 (2017) In press
REFERENCE   2  (bases 1 to 409919)
  AUTHORS   Pan,G., Xu,Z. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUL-2017) Chemistry, The Scripps Research Institute,
            130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 04-APR-2016
            Assembly Method                   :: CLC NGS Cell v. 7.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002803145.1-RS_2024_08_14
            Annotation Date                   :: 08/14/2024 03:20:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,223
            CDSs (total)                      :: 7,128
            Genes (coding)                    :: 7,004
            CDSs (with protein)               :: 7,004
            Genes (RNA)                       :: 95
            rRNAs                             :: 8, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 32 of 124
            Pseudo Genes (incomplete)         :: 102 of 124
            Pseudo Genes (internal stop)      :: 10 of 124
            Pseudo Genes (multiple problems)  :: 18 of 124
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:31:40
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 175457
            Orig. end                         :: 186258
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NNBO01000006.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10801
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10801
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10801
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5801](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5801)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    998..1012
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.97"
                     /tool="antismash"
     gene            1089..2372
                     /locus_tag="CG736_RS15930"
                     /old_locus_tag="CG736_15875"
     CDS             1089..2372
                     /GO_function="GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135058.1"
                     /locus_tag="CG736_RS15930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CG736_15875"
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="WP_232734021.1"
                     /transl_table=11
                     /translation="MAQVPTRPIRWSRPATWPREVLVLAVYWIASRLLMVGLVRSAKTE
                     STSEVGELYRGWSEVLRTGSFPVDDVTWQYPPGAALPMLAPAALPFLTYLQAFWLLMLL
                     TDALVQFVLLRVSLGRPGRSTSGAWLWALALPLLLGLPYGRYDLAVTGLAMVSLLLLPA
                     RPRLGGALAGVGAMVKVWPALIVLGTPRGRTTRQAWTAMAAAALALLLALAALFDHALD
                     FLHAQRHRGVEIESLGGTAFQLARMFGWPGRVEQHYGSMEFLGPYVHRASQLSLLLTVA
                     AFGWLLLWRLRARRWTAATPYDAALSAVLLFTLTSRVISPQYLIWLIGLAAVCLSVRGT
                     CQRPVALLLVPVVALTSVDFPLFFDAAVAGGWRPTAVIVARNVLLLAAGLWSAGRLWRA
                     TVPSRARRRRTAPAPTGPRTDTDPVGTV"
     gene            2403..2786
                     /locus_tag="CG736_RS15935"
                     /old_locus_tag="CG736_15880"
     CDS             2403..2786
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003994965.1"
                     /locus_tag="CG736_RS15935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CG736_15880"
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="WP_043913224.1"
                     /transl_table=11
                     /translation="MADTPLDRLAAGKYLLITSYRKDGSGVPTPVWMVRDGDSLGVWTV
                     ADSWKVKRIRRRPDVLVGPCDVRGNPTGEAVPATAAVADAATTEHYRRLLARKYGLLWW
                     VTLLGSRLRRGKHGTVGLTVRLA"
     gene            complement(2794..3570)
                     /locus_tag="CG736_RS15940"
                     /old_locus_tag="CG736_15885"
     CDS             complement(2794..3570)
                     /EC_number="3.1.-.-"
                     /GO_function="GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135060.1"
                     /locus_tag="CG736_RS15940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CG736_15885"
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="WP_232734022.1"
                     /transl_table=11
                     /translation="MRQYRAMVAVGDSFTEGMCDDVLPDGQFRGWADRVAGALAAEQDG
                     FRYANLAVRGKLIGQIRDEQVDRAAATDADLVTLAGGLNDVLRPGCDVAEVERRLGECA
                     AALVADGRTVVLFTSTDPTRRMAGSARLLPAILRMKAFVERTVRENPGRVVLVDLFSAP
                     AFDDRRLWAEDRLHLSAEGHRRVALAVLEALGRPSEEDWRAPLAEPDTRTRGEKLRADA
                     RWLRTHVGPWIGRRLRGRSSGDGRPAKRPELLPYRV"
     gene            complement(3605..4861)
                     /locus_tag="CG736_RS15945"
                     /old_locus_tag="CG736_15890"
     CDS             complement(3605..4861)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135063.1"
                     /locus_tag="CG736_RS15945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CG736_15890"
                     /product="cytochrome P450"
                     /protein_id="WP_100586880.1"
                     /sec_met_domain="p450 (E-value: 6.6e-19, bitscore: 62.4,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDSSEPRCPFLDPTAGAIHAEAAGLRVQGPAVLVELPGQVRAWS
                     VTRHAVIQALTTDPRVSRDFRRHWPGVGEVPDGWVLGSVAFQDSLVNRYGDEHRRARGR
                     IAPSFLPRRVKPMRPQVQATADRLVAALGALAPGERADLRGALARPLTMTLICDLFGVP
                     EERRERLGHLIDRALDSSLAEDAALAVQNELYERLGELATYKRAHPGADLTTDLLLSDR
                     PGEQPLAEHELVGTLFTMIAAGFETAVNLIASAALLVLTHPEQLAGLRAGRIDWTDVIE
                     ETLRVEGPVMYVPLRYALEDIDLGEGVVIAKGDPIMVGFAAAGRDPQEHPDRPDEFDPT
                     RADKGHLAFGHGPHFCVGAPLARLEAEVALSTLFERLPELALAEPGRRPARLPSLIVNG
                     PERVEVVPRPRTGGDRTRE"
     gene            complement(5001..5801)
                     /locus_tag="CG736_RS15950"
                     /old_locus_tag="CG736_15895"
     CDS             complement(5001..5801)
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135064.1"
                     /locus_tag="CG736_RS15950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CG736_15895"
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_100586881.1"
                     /sec_met_domain="Linocin_M18 (E-value: 8.9e-96, bitscore:
                     314.4, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNLHRELAPVSAAAWAEIEEEARRTFTLHLAGRRVVDLLGPDGP
                     ELAAVGTGHLADIAAPTNGVQARARECRPLVELRVPFTVTRAAVDDVERGSKDSDWDPV
                     KDAARTIALAEDRALFDGYAAAGITGIRDGSSNHPVALPADVRDYPNAVSRALTALRLA
                     GVGGPYSLLLGADTYTAVNETSDHGYPVHNHLARLLDGEIIWAPAVEGAILLSTRGGDF
                     ELHLGEDLSIGYLSHDDTTVHLYLQESLTFQLHTPEASVSLTSA"
     gene            complement(5798..6856)
                     /locus_tag="CG736_RS15955"
                     /old_locus_tag="CG736_15900"
     CDS             complement(5798..6856)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135065.1"
                     /locus_tag="CG736_RS15955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CG736_15900"
                     /product="Dyp-type peroxidase"
                     /protein_id="WP_100586882.1"
                     /transl_table=11
                     /translation="MPQPQPVLADLTGAAVFLVLTVEEGGEQAVRDLLPDLGGLARSVS
                     TRAPHDGLSLVVGIGSDAWDRLFAGPRPAGLHPFEELAGPRHLAPATPGDLLLHVRAER
                     MDLCFELATRITGRLGAAVSVVDETHGFRYFDCRDLIGFVDGTENPVGRAAADAVLIGD
                     EDPAFAGGSYVVVQKYLHDMDGWNALSTERQELIIGRTKLDNIELDDAVKPADSHVALN
                     TVTGPDGEGVDIWRLNMPFGSFTEGGECGTYFIGYARDPWVTEEMLRNMFLGRGPAAHD
                     RLLDFSTPVTGTLFHVPSADFLSDPPPAPGTAAARAQADAPSAPQPPAPSANPAVRDGS
                     LRIGSLKRSPSP"
     gene            complement(6973..7479)
                     /locus_tag="CG736_RS15960"
                     /old_locus_tag="CG736_15905"
     CDS             complement(6973..7479)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CG736_RS15960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="CG736_15905"
                     /product="hypothetical protein"
                     /protein_id="WP_100586883.1"
                     /transl_table=11
                     /translation="MQETDRSRALAGFTAAAVGTGIGLALILLFWAGSALFGDGGLPSS
                     IVGLTILPVGGPISSIWERRPVNRRPALLVALLLFLLVTVVLAALGDTYAEDGEAGLGW
                     AAFGHLPGVLLAGAVFGALCGEAADEPGRHWLRFTGRSRRLHLAVYGGLAVLSALAVAA
                     MVASG"
     gene            complement(7510..8001)
                     /locus_tag="CG736_RS15965"
                     /old_locus_tag="CG736_15910"
     CDS             complement(7510..8001)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018491055.1"
                     /locus_tag="CG736_RS15965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CG736_15910"
                     /product="hypothetical protein"
                     /protein_id="WP_100586884.1"
                     /transl_table=11
                     /translation="MDDSSRMWITAVPPFGPDDVGVLLSVDTDSADPGERIACQLLNRG
                     HEGEEGVFYLLPFDLSARYARTGDRLSVALVAPRAVLGEELAEQAELLDAFADAPTEGE
                     EEWVTLLRRELVTDFVPAEQDGEKQAVLLIDHVGPVDTPAGLFDRFEAGESGMAVLNAQ
                     "
     misc_feature    8087..8105
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 21.55"
                     /tool="antismash"
     misc_feature    8088..8104
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 25.33"
                     /tool="antismash"
     misc_feature    complement(8188..8199)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: strong, score: 21.4"
                     /tool="antismash"
     gene            complement(8235..8417)
                     /locus_tag="CG736_RS15970"
                     /old_locus_tag="CG736_15915"
     CDS             complement(8235..8417)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CG736_RS15970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="CG736_15915"
                     /product="hypothetical protein"
                     /protein_id="WP_100586885.1"
                     /transl_table=11
                     /translation="MPRLIEIAVSAARAAARPFRSRRPAHDHAEAHRAEVARDAAAARH
                     HRSANHSWTGGGPAQ"
     gene            complement(<8501..8860)
                     /locus_tag="CG736_RS15975"
                     /old_locus_tag="CG736_15920"
                     /pseudo=""
     CDS             complement(<8501..8860)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1167:
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088500.1"
                     /locus_tag="CG736_RS15975"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="CG736_15920"
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /pseudo=""
                     /transl_table=11
                     /translation="MPRRSYDQYCAVARALDAVGERWSLLIVRELLGGPRRYTDLHADL
                     PGVSTDILATRLKQLEAEGLIERRRLERPANATVYQLTDRGAELRPVIEALAHWGAPEL
                     GEPRPTDAFRGHWYAV"
     gene            8960..10171
                     /locus_tag="CG736_RS15980"
                     /old_locus_tag="CG736_15925"
     CDS             8960..10171
                     /EC_number="2.6.1.-"
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (33-394). E-value:
                     2.3e-52. Score: 170.2. Matches aSDomain:
                     nrpspksdomains_CG736_RS15980_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019: aminotransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003969844.1"
                     /locus_tag="CG736_RS15980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CG736_15925"
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="WP_100586887.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 1.4e-51, bitscore:
                     170.2, seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 3.9e-08,
                     bitscore: 27.4, seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEFRQSSKLKDVCYEIRGPVVDTANALEEAGHSVLRLNTGNPAPF
                     GFEAPEEIVQDIIRNLTNAHGYSDSRGILPARRAVVQYYQQRGVPSVTVDDVYLGNGVS
                     ELIQMSVQALVDNGDEVLVPMPDYPLWTAVVTFAGGKAVHYLCDEESDWFPDLDDIAAK
                     ITDRTKAIVVINPNNPTGAVYPKELLEGLLDLARRHNLMVFADEIYDKILYDGVEHHCL
                     AALADDVLTLTFNGLSKSYRVAGFRSGWLVVSGPKQHAKDYLEGLTMLAGMRLCPNVPA
                     QYAVQAALGGRQSIDDLVLPNGRLTEQRDVTWRALNEIPGVSCVKPRGALYAFARLDPA
                     VHRIVDDERFVLDLLLREKIHIVQGTGFNWPRPDHFRFVTLPRADDLETAINRIGRFLA
                     TYRQ"
     aSDomain        9059..10141
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CG736_RS15980_Aminotran_1_2.1"
                     /evalue="2.30E-52"
                     /label="CG736_RS15980_Aminotran_1_2.1"
                     /locus_tag="CG736_RS15980"
                     /protein_end="394"
                     /protein_start="33"
                     /score="170.2"
                     /tool="antismash"
                     /translation="VLRLNTGNPAPFGFEAPEEIVQDIIRNLTNAHGYSDSRGILPARR
                     AVVQYYQQRGVPSVTVDDVYLGNGVSELIQMSVQALVDNGDEVLVPMPDYPLWTAVVTF
                     AGGKAVHYLCDEESDWFPDLDDIAAKITDRTKAIVVINPNNPTGAVYPKELLEGLLDLA
                     RRHNLMVFADEIYDKILYDGVEHHCLAALADDVLTLTFNGLSKSYRVAGFRSGWLVVSG
                     PKQHAKDYLEGLTMLAGMRLCPNVPAQYAVQAALGGRQSIDDLVLPNGRLTEQRDVTWR
                     ALNEIPGVSCVKPRGALYAFARLDPAVHRIVDDERFVLDLLLREKIHIVQGTGFNWPRP
                     DHFRFVTLPRADDLETAINRI"
     gene            complement(10327..10755)
                     /locus_tag="CG736_RS38610"
                     /old_locus_tag="CG736_15930"
     CDS             complement(10327..10755)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015718.4"
                     /locus_tag="CG736_RS38610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CG736_15930"
                     /product="chaplin"
                     /protein_id="WP_100586888.1"
                     /transl_table=11
                     /translation="MTNSRKYLAAAGLTAALVVLPTSSATAHGHGSGHAASASPVGTLC
                     INSGDAVANGIAAGSPGVLSGNQIQIPVNIQANVCGNTIDIVGLLNPGGGGGNGGGGNN
                     GGGNGGGGNNGGGNNGGGNNGGGSGNNGGGNSGGGANS"
ORIGIN
        1 aggcccagcg gacggtgtcg gtgagccact tgtcggcggg gcggccgcgg tcggcgatgc
       61 cgaaccgggc ggagatccgc tgctcgtagt cgctcgcgcc gggcaggtcg gcctcctcgg
      121 cgccgtcgta cggggagagt tcgaggtcga gggcctcttc cttctcgccg cgcagcatcg
      181 ccaggatccg gtgcgagggc agcttggtgt acggctcggc gaagtcgaag tagtcggcga
      241 acttggcgcc gtcctgctcc ttgccgtcgc ggaccttggc gaccagccgg ccgcgggtcc
      301 acatccgttc gcgcagcgag ccgaccaggt cggcgtcctc gccgaagcgt tcgaccagga
      361 tggcgcgggc gccctccagc gcggcggcgg cgtcggcgac ctgctcgttc gcgtagccgg
      421 cggcgagcgc ggccgggtcc tgcgcggggt cggcgagcag cgtgtcggcc agcggttcga
      481 ggccggcctc gcgggcgatc tgcgccttgg tgcgccgctt gggcttgtac gggaggtaga
      541 tgtcctccag ccgggccttg gagtcggcgg cgaggatctg cgcgcgcagc gtgtcgtcga
      601 gcttgccctg ggagtcgatg gactccagga cggcggtccg gcgctcctcg agttcgcgca
      661 ggtagcgcag ccgctcctcc agggtgcgca gctgggtgtc gtcgagggca ccggtcgctt
      721 ccttgcggta gcgcgcgatg aaggggacgg tcgctccgcc gtcgagcagg ccgacggcgg
      781 cctgcacctg gttctcgcgg acgcccagtt cctcggcgat cttgcgttcg acgggcatgg
      841 tcacgatgcc ggtcaccctt tctggtggtt tcgcttctcg cccgtgcatt ctggcaggtc
      901 cggccgacgt tgttcggcgg accgggcggc acggcggggt gtggcgcgtt ccggggacgt
      961 gcacggacac gcgcggaggc gggtccggcg tcgcccgatt tgtctgatat gagtgagatc
     1021 gcacaagggc ctgtccagag gctcggcccc ggatcgaccc cgcagcacga ccgcacagga
     1081 ggacaacggt ggcgcaggtc cccacccggc cgatccgctg gagccgcccc gcgacctggc
     1141 cccgcgaggt cctcgtgctg gccgtgtact ggatcgccag ccggctgctg atggtcggcc
     1201 tggtccgctc cgcgaagacc gagtccacct ccgaggtcgg cgagctgtac cgcggctggt
     1261 ccgaggtgct gcgcaccggc tccttcccgg tggacgacgt cacctggcag tacccgccgg
     1321 gcgcggcgct gccgatgctc gcacccgccg cgctgccgtt cctgacgtac ctccaggcgt
     1381 tctggctgct gatgctgctc accgacgcgc tggtccagtt cgtgctgctg cgggtctcgc
     1441 tgggccggcc cggccgttcc acctccggcg cgtggctgtg ggcgctggcg ctgccgctgc
     1501 tgctcggcct gccgtacggg cgctacgacc tggcggtcac cgggctggcg atggtctcgc
     1561 tgctgctgct gcccgcccgg ccccggttgg gcggtgcgct ggccggggtc ggcgcgatgg
     1621 tcaaggtgtg gccggcgctg atcgtgctcg gcaccccgcg cggccgcacc acccggcagg
     1681 cctggacggc gatggcggcg gccgcgctcg ccctgctgct ggcgctggcc gcgctcttcg
     1741 accacgcctt ggacttcctg cacgcgcagc gccaccgcgg cgtcgagatc gagtcgctgg
     1801 gcggcacggc cttccagctg gcccggatgt tcggctggcc cggccgggtc gagcagcact
     1861 acggctcgat ggagttcctc ggcccgtacg tccaccgggc gtcccagctg tcgctgctgc
     1921 tgacggtcgc cgcgttcggc tggctgctgc tgtggcggct gcgcgcccgc cgctggacgg
     1981 ccgccacccc gtacgacgcg gcgctcagcg ccgtgctgct gttcaccctg accagccggg
     2041 tgatctcccc gcagtacctg atctggctga tcgggctggc cgcggtctgc ctgtccgtcc
     2101 ggggcacctg ccagcggccg gtggcgctgc tgctggtccc ggtggtcgcg ctgacctccg
     2161 tcgacttccc gctgttcttc gatgcggcgg tggccggcgg ctggcggccc accgcggtga
     2221 tcgtggcccg caacgtgctg ctgctggccg cgggcctgtg gtcggcggga cggctgtggc
     2281 gggccaccgt cccgtcccgg gcccgccgtc ggcgcaccgc gcccgcgccc accgggcccc
     2341 ggacggacac cgacccggtc ggcactgtct gacggaccgt cggccgccgt acactcgcgg
     2401 ccatggccga caccccgctg gaccggctcg ccgccgggaa gtacctgctc atcaccagtt
     2461 accgcaagga cggcagcggc gtgccgacgc cggtgtggat ggtccgcgac ggcgacagcc
     2521 tcggcgtgtg gaccgtggcc gactcgtgga aggtcaagcg gatccgccgg cgcccggacg
     2581 tcctggtcgg gccgtgcgac gtgcgcggca acccgaccgg cgaggcggtg ccggccaccg
     2641 ccgcggtcgc cgacgccgcc accaccgagc actaccggcg gctgctcgcc cgcaagtacg
     2701 ggctgctctg gtgggtcacc ctgctcggca gccggctgcg ccgcggcaag cacggcaccg
     2761 tcggcctcac cgtccggctc gcctgacgtt ccgtcacacc cggtacggca gcagctcggg
     2821 gcgcttggcg gggcgcccgt cgccggagga gcggccgcgc agccgccgcc cgatccacgg
     2881 gccgacgtgg gtgcgcagcc agcgggcgtc cgcgcgcagc ttctcgcccc gggtgcgggt
     2941 gtcgggctcg gccagcggcg cgcgccagtc ctcctcggac gggcggccga gcgcctccag
     3001 caccgccagc gcgacccggc ggtggccctc cgccgacagg tgcagccggt cctccgccca
     3061 cagccggcgg tcgtcgaagg ccggcgcgga gaacaggtcg accagcacca cccggcccgg
     3121 gttctcccgc accgtccgct ccacgaacgc cttcatccgc aggatcgccg gcagcagccg
     3181 cgcgctgccg gccatccgcc gggtcgggtc ggtcgaggtg aacagcacca cggtgcggcc
     3241 gtccgcgacc agtgccgccg cgcactcgcc gagccgccgc tccacctcgg ccacgtcgca
     3301 gcccggccgc agcacgtcgt tcaggccgcc cgcgagcgtc accaggtcgg cgtcggtcgc
     3361 cgccgcccgg tccacctgct cgtcccggat ctggccgatc agcttgcccc gcacggcgag
     3421 attcgcgtac cggaacccgt cctgctccgc ggccagcgcg cccgcgaccc ggtccgccca
     3481 gccgcggaac tgcccgtcgg gcaggacgtc gtcgcacatc ccctcggtga acgagtcgcc
     3541 caccgccacc atcgcccggt actgccgcac accgcctcct ggggtcgtcc ctgcggcccc
     3601 aaccctactc gcgggttcgg tcgccgccgg tgcgcggccg gggcaccacc tccacacgct
     3661 ccgggccgtt gacgatcagc gacgggaggc gggcgggccg ccgccccggc tccgccaggg
     3721 ccagctcggg cagccgctcg aacagggtgc tcagcgccac ctcggcctcc agtcgggcca
     3781 gcggcgcgcc gacgcagaag tgcgggccgt ggccgaacgc caggtggccc ttgtccgccc
     3841 gggtcgggtc gaactcgtcc ggccggtccg ggtgctcctg cgggtcccgg cccgcggcgg
     3901 cgaacccgac catgatcggg tcgcccttcg cgatcaccac gccctcgccg aggtcgatgt
     3961 cctccagcgc gtaccgcagc gggacgtaca tgacggggcc ctcgacccgc agcgtctcct
     4021 cgatgacgtc cgtccagtcg atccgccccg cccgcagccc ggccagttgc tcggggtggg
     4081 tgagcaccag cagcgccgcg ctggcgatca ggttgacggc cgtctcgaag ccggcggcga
     4141 tcatcgtgaa cagcgtgccg accagttcgt gctccgccag cggctgttcg cccggccggt
     4201 cggagagcag caggtcggtg gtcaggtcgg cgcccgggtg ggcccgcttg tacgtcgcca
     4261 actcgccgag gcgttcgtag agttcgttct gcacggcgag cgcggcgtcc tcggccagcg
     4321 aggagtccag cgcccggtcg atcaggtggc ccagcctctc gcgcctctcc tcgggcacgc
     4381 cgaacaggtc gcagatcaac gtcatcgtca gcggccgcgc caacgccccc cgcaggtccg
     4441 cgcgttcacc cggcgcgagc gctccgagcg ccgccaccag ccggtcggcc gtcgcctgca
     4501 cctggggccg catcggcttc acccgccgcg gcaggaacga cggcgcgatc cgcccccggg
     4561 ccctgcggtg ctcgtccccg taccggttca ccaggctgtc ctggaacgcc accgacccca
     4621 gcacccaccc gtccggcacc tcgccgactc ccggccagtg ccgccggaag tcccgcgaca
     4681 cccgcggatc ggtggtcagc gcctggatca ccgcgtgcct ggtcaccgac caggcccgga
     4741 cctgcccggg caactcgacc agcaccgccg gcccctgcac ccgcaacccg gcggcctccg
     4801 cgtggatcgc ccccgcggtg gggtcgagaa acgggcatcg cggctcggaa gaatcggaca
     4861 cggcgtgctc ccctcagcac cggacgtccc tgcgtgcctc atatggtgcg ccccccgccg
     4921 gagacccgga agcctccctt cgccattggc ctgattccat ccctcttggc gtcaactccc
     4981 gttccgatca ccccagttag tcaggcggag gtgagcgaca ccgaggcctc gggggtgtgg
     5041 agctggaagg tgagggactc ctggaggtag aggtggacgg tggtgtcgtc gtgggagagg
     5101 tagccgatcg acaggtcctc gccgaggtgg agttcgaagt cgccgccgcg ggtggagagc
     5161 aggatggcgc cctcgacggc gggggcccag atgatctcgc cgtcgaggag gcgggcgagg
     5221 tggttgtgga cggggtagcc gtggtcggag gtctcgttga cggcggtgta ggtgtcggcg
     5281 ccgagcagca gggagtaggg gccgccgacg ccggccaggc gcagcgcggt gagggcgcgg
     5341 ctgacggcgt tggggtagtc gcggacgtcg gcgggcaggg cgacggggtg gttgctggag
     5401 ccgtcgcgga tgccggtgat gccggcggcg gcgtagccgt cgaagagggc gcggtcctcg
     5461 gcgagggcga tggtgcgggc ggcgtccttg accgggtccc agtcggagtc cttggagccg
     5521 cgttcgacgt cgtcgacggc ggcccgggtg acggtgaacg ggacgcgcag ttcgaccagc
     5581 ggccggcact cccgggcgcg ggcctggacg ccgttggtgg gggccgcgat gtcggcgagg
     5641 tggccggtgc cgaccgcggc gagttccggc ccgtcggggc cgagcaggtc gaccacccgg
     5701 cggccggcca ggtgcagggt gaaggtccgc cgggcctcct cctcgatttc ggcccaggcg
     5761 gcggcggaga cgggcgccag ctcgcggtgc aggttgttca cggtgaggga ctcctcttca
     5821 ggctgccgat gcggagactg ccgtcccgga ccgccgggtt cgcggacggg gccggtggct
     5881 ggggtgccga cggggcgtcg gcctgcgcgc gggcggccgc ggtgccgggc gcgggcggcg
     5941 ggtcgctcag gaagtcggcg ctcgggacgt ggaagagggt cccggtgacg ggggtggaga
     6001 agtccagcag ccggtcgtgg gcggccggtc cgcggccgag gaacatgttg cggagcatct
     6061 cctcggtcac ccacgggtcg cgggcgtagc cgatgaaata ggtgccgcac tcgccgccct
     6121 cggtgaacga gccgaacggc atgttcagcc gccagatgtc gaccccttcg ccgtccggcc
     6181 cggtgacggt gttcagcgcg acgtgcgagt cggcgggctt gacggcgtcg tccagttcga
     6241 tgttgtcgag cttggtgcgc ccgatgatca gttcctggcg ttcggtgctg agcgcgttcc
     6301 aaccgtccat gtcgtgcagg tacttctgca cgacgacgta ggagccgccg gcgaacgccg
     6361 ggtcctcgtc gccgatcagc actgcgtcgg cggcggcccg gccgaccggg ttctcggtgc
     6421 cgtcgacgaa gccgatcagg tcgcggcagt cgaagtaccg gaagccgtgc gtctcgtcga
     6481 cgacgctcac cgcggcgccc agccggccgg tgatccgggt ggccaactcg aagcacaggt
     6541 ccatgcgttc ggcgcggacg tgcagcagca ggtcgccggg ggtggcgggg gcgaggtgcc
     6601 gggggccggc cagttcctcg aatgggtgca ggccggcggg gcggggcccg gcgaacaggc
     6661 ggtcccaggc gtccgagccg atgccgacca ccagcgacag cccgtcgtgc ggggcgcggg
     6721 tgctcaccga gcgggccagc ccgccgaggt cgggcagcag gtcgcggacg gcctgttcgc
     6781 cgccctcctc gacggtgagc accaggaaca cggcggcccc ggtcagatcg gcgaggaccg
     6841 gttggggctg cggcatggcg gatgctccag ggggacgtgc ggacgcccgc cgaccatacc
     6901 gggcaaaagg ttggagactc tgccatagcg cctgaatcgg gcgtgtcccg gacctggacc
     6961 gagccccgcg cgtcagccgc tggcgaccat cgccgcgacg gcgagtgcgg acaggaccgc
     7021 caggccgccg tacacggcga ggtggaggcg gcggctgcgg ccggtgaacc gcagccagtg
     7081 ccgacccggt tcgtcggccg cttcgccgca cagcgcgccg aagaccgcgc cggccaacag
     7141 cacgccgggg aggtggccga acgccgccca accgaggccc gcctcgccgt cctcggcgta
     7201 ggtgtcgccg agggcggcca ggaccacggt gaccagcagg aacaggagca gtgcgaccag
     7261 cagggcgggc cgccggttca ccggccggcg ttcccagatc gagctgatcg gaccgcccac
     7321 cggaaggatc gtcaggccca cgatcgacga cgggagcccg ccgtcgccga acagcgcaga
     7381 gccggcccag aacagcagga tcagggccag cccgatgccc gtcccgacgg ctgcggccgt
     7441 gaacccggcc agcgcgcgcg accgatcggt ctcctgcacg agtctccccc gttcccacat
     7501 cccgccccgc tactgggcgt tgagcaccgc catccctgat tcccccgcct cgaagcggtc
     7561 gaacagcccg gccggcgtat ccaccggccc gacgtggtcg atcagcagca ccgcctgctt
     7621 ctcgccgtcc tgctcggccg gcacgaagtc ggtgaccagt tcgcgccgca gcagcgtcac
     7681 ccactcctcc tcgccctcag tcggggcatc ggcgaacgcg tccaacagct cggcctgttc
     7741 ggccagttcc tcgccgagca ccgcccgcgg cgcgaccagc gcgacggaca gccggtcgcc
     7801 ggtccgggcg tagcgggcgg acaggtcgaa gggcagcagg tagaacacgc cctcctcgcc
     7861 ctcgtggccg cggttcagca gctgacaggc gatccgctcc ccggggtcgg ccgagtcggt
     7921 gtccaccgac agcagcaccc cgacgtcgtc cgggccgaac ggcggtacgg cggtgatcca
     7981 catgcgtgag gagtcgtcca tggcggccag ccaagcacac ggctccgacg gcccgccgcg
     8041 cgctccgggt cggccgccca cttcgatccg acgagcagtc agtccggctg cgaacgacgc
     8101 gcaaggacca cctcggcacc gcgcccgccc ggtcgatggc ccgctccggc cgtcggcgac
     8161 ggggcgcagc ccgacccctc ccggagggcg ttcatgtccc gttccaccct cgccgcgaca
     8221 cctgtctacc gccgctactg ggccggcccg ccgcccgtcc agctgtggtt ggcggaccgg
     8281 tggtggcggg ccgccgcggc gtcccgggcg acctcggcgc ggtgggcctc ggcgtggtcg
     8341 tgggcggggc gccgactgcg aaaggggcgg gcggccgcgc gggcggcgga gacggcgatt
     8401 tcgatcagcc gtggcatggt ggacccccgt tcgtctccgg acgaccggtt gcgcccggac
     8461 cttctcagcg tacccaaata gtcgccttca caagtcagtt gacggcatac cagtggccgc
     8521 ggaaggcgtc ggtggggcgg ggttcgccga gttccggggc gccccagtgg gcgagcgcct
     8581 cgatgacggg tcgcagctcg gcgccgcggt cggtgagctg gtagaccgtg gcgttggcgg
     8641 ggcgctccag ccgtcggcgc tcgatcagcc cctcggcctc cagttgcttg aggcgggtgg
     8701 ccaggatgtc ggtggagacg cccggcaggt cggcgtgcag gtcggtgtag cggcgcggtc
     8761 cgccgaggag ttcacggacg atcaggagac tccagcgttc tccgaccgcg tccagggcgc
     8821 gggcgacggc gcagtactgg tcgtagcttc ggcgtggcat gccgtcagca tagccaagag
     8881 gttggacttt ccaagtccct acttggtaga acgaagcaag ccggccgcag gggctcggtg
     8941 acgggtcggg aggccgctga tggagtttcg ccagtccagc aagctgaagg acgtctgcta
     9001 cgagatccgc ggcccggtcg tcgacaccgc caacgccctg gaggaggccg gccacagcgt
     9061 cctgcgactg aacaccggca acccggcgcc gttcggcttc gaggcccccg aggagatcgt
     9121 ccaggacatc atccgcaacc tcaccaacgc gcacggctac agcgactccc gcggcatcct
     9181 gccggcccgc cgcgccgtgg tgcagtacta ccagcagcgc ggcgtcccga gcgtcaccgt
     9241 ggacgacgtc tacctcggca acggcgtctc cgagctgatc cagatgagcg tgcaggcgct
     9301 ggtcgacaac ggcgacgagg tgctcgtccc gatgccggac tacccgctgt ggacggcggt
     9361 ggtgaccttc gccggcggca aggcggtgca ctacctgtgc gacgaggagt ccgactggtt
     9421 ccccgacctg gacgacatcg ccgccaagat caccgaccgc accaaggcga tcgtggtgat
     9481 caacccgaac aacccgaccg gcgcggtcta cccgaaggaa ctcctcgagg gcctgctgga
     9541 cctggcgcgc cgccacaacc tgatggtctt cgccgacgag atctacgaca agatcctcta
     9601 cgacggcgtc gagcaccact gcctggccgc cctcgccgac gacgtcctca ccctcacctt
     9661 caacggcctc tccaagtcct accgggtggc cgggttccgc agcggctggc tggtggtctc
     9721 cggccccaag cagcacgcca aggactacct ggagggcctc accatgctgg ccggcatgcg
     9781 cctgtgcccc aacgtgcccg cccagtacgc cgtccaggcc gcgctcggcg gccggcagtc
     9841 catcgacgac ctcgtcctgc cgaacggccg cctcaccgag cagcgcgacg tcacctggcg
     9901 cgcgttgaac gagatccccg gcgtctcctg cgtcaagccg cgcggcgcgc tgtacgcctt
     9961 cgcccggctc gaccccgccg tccaccggat cgtcgacgac gagcgcttcg tcctcgacct
    10021 gctgctccgc gagaagatcc acatcgtcca gggcaccggc ttcaactggc cccgccccga
    10081 ccacttccgc ttcgtcaccc tcccccgggc cgacgacctg gagacggcca tcaaccgcat
    10141 cggccgcttc ctcgccacct accgccaata gccgcggcgg cggtcagccg acggccgggg
    10201 cgcccgccgt cccggccggg ccccgaccgt ccatgcccgg cagggccgtc cccggcacgc
    10261 ctccacaccg ctgcgcccca ctccgaggcc attgaccagc aggaacgaag aggcgcagct
    10321 ggattactag ctgttcgcgc ccccgccgct gttgcccccg ccgttgttcc cgctcccacc
    10381 gccgttgttg cccccgccgt tgttgccccc gccgttgttc cctcccccac cgttgccgcc
    10441 tccgttgttg ccgccgccgc cgttgccgcc tccgccgccc gggttgagca ggccgacgat
    10501 gtcgatggtg ttgccgcaga cgttggcctg gatgttgacg gggatctgga tctggttgcc
    10561 ggacagcacg cccggcgatc cggcggcgat gccattggcg accgcgtcgc ccgagttgat
    10621 gcacaacgtg cccacgggcg aggcggacgc ggcgtggccg gaaccgtgtc catgggcggt
    10681 ggcggagctc gtggggagga cgacaagcgc ggcagtgagg ccggcagctg cgaggtactt
    10741 cctggagttg gtcatgcaaa ggaactatcc gatcatcctc cccaacccgg tgagacgcgg
    10801 c
//
