LOCUS       NZ_BEWI01000032        20612 bp    DNA     linear   CON 12-AUG-2024
DEFINITION  Sphingobium fuliginis strain OMI SOMI032, whole genome shotgun
            sequence.
ACCESSION   NZ_BEWI01000032
VERSION     NZ_BEWI01000032.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Sphingobium fuliginis
  ORGANISM  Sphingobium fuliginis
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Sphingobium.
REFERENCE   1
  AUTHORS   Ogata,Y., Toyama,T., Yu,N., Wang,X., Sei,K. and Ike,M.
  TITLE     Occurrence of 4-tert-butylphenol (4-t-BP) biodegradation in an
            aquatic sample caused by the presence of Spirodela polyrrhiza and
            isolation of a 4-t-BP-utilizing bacterium
  JOURNAL   Biodegradation 24 (2), 191-202 (2013)
   PUBMED   22777343
REFERENCE   2
  AUTHORS   Ogata,Y., Goda,S., Toyama,T., Sei,K. and Ike,M.
  TITLE     The 4-tert-butylphenol-utilizing bacterium Sphingobium fuliginis OMI
            can degrade bisphenols via phenolic ring hydroxylation and
            meta-cleavage pathway
  JOURNAL   Environ Sci Technol 47 (2), 1017-1023 (2013)
   PUBMED   23215053
REFERENCE   3
  AUTHORS   Takada,K., Shiba,T., Soda,S., Inoue,D., Miyake,M., Eguchi,M. and
            Ike,M.
  TITLE     Bioaugmenting a lab-scale membrane bioreactor with Sphingobium
            fuliginis OMI to degrade 4-tert-butylphenol
  JOURNAL   Unpublished
REFERENCE   4  (bases 1 to 2787552)
  AUTHORS   Kuroda,M., Ogata,Y., Inoude,D., Sei,K. and Ike,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-2017) Contact:Masashi Kuroda Osaka University,
            Graduate School of Engineering; Yamadaoka 2-1, Suita, Osaka
            565-0871, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Celera Assembler v. 5.3
            Genome Coverage                   :: 20x
            Sequencing Technology             :: Roche GS FLX Titanium Chemistry
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002723855.1-RS_2024_08_12
            Annotation Date                   :: 08/12/2024 02:52:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,410
            CDSs (total)                      :: 5,341
            Genes (coding)                    :: 5,170
            CDSs (with protein)               :: 5,170
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 171
            CDSs (without protein)            :: 171
            Pseudo Genes (ambiguous residues) :: 0 of 171
            Pseudo Genes (frameshifted)       :: 49 of 171
            Pseudo Genes (incomplete)         :: 133 of 171
            Pseudo Genes (internal stop)      :: 8 of 171
            Pseudo Genes (multiple problems)  :: 15 of 171
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:08:54
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 961649
            Orig. end                         :: 982261
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BEWI01000032.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..20612
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hserlactone"
                     /region_number="3"
                     /rules="Autoind_synth"
                     /tool="antismash"
     cand_cluster    1..20612
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="Autoind_synth"
                     /kind="single"
                     /product="hserlactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20612
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[10000:10612](+)"
                     /cutoff="20000"
                     /detection_rule="Autoind_synth"
                     /neighbourhood="10000"
                     /product="hserlactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10612
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="Autoind_synth"
                     /neighbourhood="10000"
                     /product="hserlactone"
                     /protocluster_number="1"
     misc_feature    complement(339..351)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.46"
                     /tool="antismash"
     misc_feature    1504..1517
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: strong, score:
                     17.22"
                     /tool="antismash"
     gene            complement(3122..4372)
                     /locus_tag="SFOMI_RS17250"
                     /old_locus_tag="SFOMI_3475"
     CDS             complement(3122..4372)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_function="GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1029: RND family
                     efflux transporter MFP subunit"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744737.1"
                     /locus_tag="SFOMI_RS17250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SFOMI_3475"
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="WP_021245249.1"
                     /transl_table=11
                     /translation="MKTSFLVRGGILAACALLAACSGSPAANETGEEPAAKAADYERGP
                     HRGRMLRDGDFAVEMTIFEDGVEPEFHVYAYRGGKPVTPGEVQLGVELTRLGGRIDRFA
                     FTPREDYLLGGGVVHEPHSFDVKVRAVEGGRTHSWSYASYEGRTTISAEAARAGGVKSE
                     RAGPVAIAELIDMAGRIEITPEGKADVRARLPGQIVWMSGQLGQQVRKGQALLRVESSH
                     SLQTYAVPAPISGTIIEKNANVGDVTGDRALFVIADPAKLHAEFFVYPRDAERVRVGQT
                     VRVRSLSGEARIEAAVEAVLPTADLASQTLMAHVHLPESAARMFRPGMGVEGQFAVASA
                     NVPLAVRTKAIQRFRDFEVVYAKVGNTYEVRMLEIGRRTPEWTEILGGLEPGTEYVTDG
                     AFLIRADVEKSGASHDH"
     gene            complement(4372..5589)
                     /locus_tag="SFOMI_RS17255"
                     /old_locus_tag="SFOMI_3476"
     CDS             complement(4372..5589)
                     /GO_function="GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1076: RND efflux
                     system, outer membrane lipoprotein,"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077333.1"
                     /locus_tag="SFOMI_RS17255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SFOMI_3476"
                     /product="TolC family protein"
                     /protein_id="WP_235600801.1"
                     /transl_table=11
                     /translation="MLPLFLYAQAASAQNLTLSDALARVASGDPVVAATAAQLQAANAA
                     IAQADVRPRDVIGVDVEDFAGTGPYSPIDRSQTTAWYERTWERGGKREARIGAARSSVG
                     VTSARTRLRMLDLLAQVQAAWVEALSAEAAVAVAGQRLAEAERVETEVRRRVAAALDPL
                     FAAERAKTSVAQARIALDQAQAAARIARAYLAAWWGGTADYRLDTAPFTNLAAAPAAEG
                     DLPDLALLDAERAAADARVRLAETGNAGDPTARVGVRHFGQGNDVALVVGGSIPLGNRS
                     ANRGNVERARADAQAAEAEIAVARVERRREIDRLTAERAALATEIVRLDREVLPGAERA
                     VVMVRDGFRRGGTAFTFLEVSQASQAVIELRSRRIDLLRRFHLIGARLDRLTGRHLPLI
                     ASAENR"
     gene            complement(5707..6093)
                     /locus_tag="SFOMI_RS17260"
                     /old_locus_tag="SFOMI_3477"
     CDS             complement(5707..6093)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="SFOMI_RS17260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SFOMI_3477"
                     /product="hypothetical protein"
                     /protein_id="WP_146001642.1"
                     /transl_table=11
                     /translation="MTAMLCIALVFVFAGMSLSNSMDRIQHAPGAPIAHEHMLLGDLSL
                     ELDHADDHHSPQPDDSDDDPVGHLAGGHHHHADGGSGLIVPDQAPAAILALAASAGTFE
                     PQRRPLGVKARGPERPPKDLGPNA"
     misc_feature    complement(6120..6134)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 20.84"
                     /tool="antismash"
     gene            complement(6321..6887)
                     /locus_tag="SFOMI_RS17265"
     CDS             complement(6321..6887)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212754.1"
                     /locus_tag="SFOMI_RS17265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_021245252.1"
                     /transl_table=11
                     /translation="MFHPGAPLQSSSSCDIAAVLERLAQVSSRPRYAFMLLNLIAQVAG
                     PDGSAGPWIKRDNELVSLRDWLCDALTPMSHRVSRSAALADRVRRDLDRSGMLTGDAQE
                     TQRTIAEEMRARMRTTGKSNLSRAVSELVNADLMRRHYQGYHVDHRNRGGQRHAVYTLI
                     GDARGLVSATSLTKHAAQGELALMR"
     misc_feature    7016..7031
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 18.62"
                     /tool="antismash"
     misc_feature    complement(7016..7034)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: medium, score: 28.77"
                     /tool="antismash"
     misc_feature    complement(7017..7033)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 23.15"
                     /tool="antismash"
     gene            7109..7870
                     /locus_tag="SFOMI_RS17270"
                     /old_locus_tag="SFOMI_3479"
     CDS             7109..7870
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1197:
                     autoinducer-binding transcriptional regulator,"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849920.1"
                     /locus_tag="SFOMI_RS17270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SFOMI_3479"
                     /product="LuxR family transcriptional regulator"
                     /protein_id="WP_022684250.1"
                     /transl_table=11
                     /translation="MANRIRDCGRLDDLTRFAQECARAGNLGDLQGTIDAAVRELGFRW
                     FTLLHNVDLRHGGGQRLFLTTYPPAWLEEVLEERHYIEDPIHAACARTPSGLAWDRVGD
                     VLELSSRQRSILKRARDHDLAAGYSLPIRTPGEPEAIFTVARPRDEPLDAAEILTARLL
                     GSVAYDRARELLGEEIRTLAFVSLSPRQVECIAFVAQGKSDWEIAQILGLSRDTVHEYV
                     ESARRRYGVRRRTQLVLRAVRDGHLNMDALL"
     gene            7887..8630
                     /locus_tag="SFOMI_RS17275"
                     /old_locus_tag="SFOMI_3480"
     CDS             7887..8630
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1197:
                     autoinducer-binding transcriptional regulator,"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015449333.1"
                     /locus_tag="SFOMI_RS17275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SFOMI_3480"
                     /product="autoinducer binding domain-containing protein"
                     /protein_id="WP_021245254.1"
                     /transl_table=11
                     /translation="MIGLGQVQDFCAVAAIIKDSQALAGLMAEITRAMGFRYVSLVHHI
                     DLKPAVSSVHIVDYPPEWVERFQARRLYASDPIHRASHRTNIGFAWSAVQSLITLSAAD
                     RSILAEAHDAGLGDGFTVPAHIPGEVNGSCSFAMGSGDVLDQRQLPLVQLIGSFAFEAA
                     RRLSRQTPAPCEGPCLTERQAECVALVARGKTDWEISQILGIGQETVIQHVKDARDRYG
                     VTKRTLLAIRALFDGQISFADVFGR"
     gene            8948..9991
                     /locus_tag="SFOMI_RS17280"
                     /old_locus_tag="SFOMI_3481"
     CDS             8948..9991
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1242: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611893.1"
                     /locus_tag="SFOMI_RS17280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SFOMI_3481"
                     /product="IS110 family transposase"
                     /protein_id="WP_099185669.1"
                     /transl_table=11
                     /translation="MSYLGVDLHQNSITICRREADGSEVFETLTLSPADLDRFCLSLDA
                     DDEVAIEATGNSAWFRDQVLPCVGRVVVVNPSQFKVIRKSVKKTDRNDAAALALFLEKD
                     MLPETRAKTQAEAELASLAHTRDLLVKQRTRLLNKIHALHVGKGLKLKKESLGSKRGLA
                     AIDMGLFSACEQIEIAVLRETATALTASLKQLDGEIEKLASALAGYEGIVSIKGIGARS
                     AAVLLAGIGNVHDFESADKLAAYFGMVPRVSQSNETDHRGRITKRGNKLARTTLVQCSL
                     IATRYSPYLKAFYDRIRARRGTGKAIIATARKLLAIIYDTLKNGWVFEDFTQFQRRENP
                     VRAGQSS"
     misc_feature    10001..10013
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 19.6"
                     /tool="antismash"
     gene            10001..10612
                     /locus_tag="SFOMI_RS17285"
                     /old_locus_tag="SFOMI_3482"
     CDS             10001..10612
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hserlactone: Autoind_synth"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683387.1"
                     /locus_tag="SFOMI_RS17285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SFOMI_3482"
                     /product="acyl-homoserine-lactone synthase"
                     /protein_id="WP_021245255.1"
                     /sec_met_domain="Autoind_synth (E-value: 6.4e-22, bitscore:
                     75.1, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHIATGAARAMENRLFCSMFEERKRVFVDLLRWDVPVFAGRYEID
                     QFDNDRAVYIVIADAEGEHLASARLLPTTGDHILGTIFPELCEQVPPRGGAILEITRFC
                     LARRLRARERLEVRNRLVSALVEYALGNHIDSYTGVAEWFWFQQILSFGWACRPLGLPA
                     STSSRCLIALQIDINGSTREQLQASGIWRSVGLADLAKAA"
     gene            10623..11570
                     /locus_tag="SFOMI_RS17290"
                     /old_locus_tag="SFOMI_3483"
     CDS             10623..11570
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015449335.1"
                     /locus_tag="SFOMI_RS17290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SFOMI_3483"
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="WP_099186291.1"
                     /sec_met_domain="PhyH (E-value: 1.4e-21, bitscore: 75.0,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTNLSPYRPAAEPAVDDDLQRHGFCVLRDIASKTEVAEIDQALDP
                     RFAATPFCQGGFYGPLTKRFGGLLKRVPTVERLVRHPAIMRLVRSVLDPWCDTIQLNLT
                     QALELHPGAPAQFPHRDQDMWRGPAGELEYLVNVMWPISPFKAENGATLIWSGSHNAGS
                     MVKDAQPMAVEAEPGSAILFLGSTLHGAGANMTGAIRRGIIVSYCLGWLKPYENKWLTY
                     PPNAARAFDPDLAALVGYQQHRPNLGNYDGQCPSVLLRGEPPEYLAATDALRPDQESAL
                     AEFLDQQRTSLLPPAGGIAEIGAGRNKREVKSNG"
     gene            11621..11962
                     /locus_tag="SFOMI_RS17295"
     CDS             11621..11962
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015449336.1"
                     /locus_tag="SFOMI_RS17295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_021245257.1"
                     /transl_table=11
                     /translation="MQRVTLSLAVLAMGTALQPTPARANDLGCQVLLCLSNPGGATQYA
                     QCVAPMTKLWQRLATGGSFPGCSGGGVARTKVHDRDSATRRRVVMTFSDGRRETYSLAH
                     IERLQDLAR"
     gene            11959..12627
                     /locus_tag="SFOMI_RS17300"
                     /old_locus_tag="SFOMI_3484"
     CDS             11959..12627
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039071.1"
                     /locus_tag="SFOMI_RS17300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SFOMI_3484"
                     /product="lytic transglycosylase domain-containing protein"
                     /protein_id="WP_021245258.1"
                     /transl_table=11
                     /translation="MILDVASLLALAGSCAPSVAPETLISIVHTESRFNTLAIGVNSPG
                     ARKPVPATRAQAIAAARRLIRQGYNIDLGLGQINSANLGWLGLSVEDAFEPCRNLAAAA
                     RVLTTNYQSVAHSAQSSDHAIATALSLYNTGDRRRGFRNGYVARVYASASTVIPLIRGR
                     HPASVMTAPVRLADAVIAKPQPTALAATQAAGWNIAADARQASLMVFGDGTTSQKGQHP
                     "
     gene            12624..12962
                     /locus_tag="SFOMI_RS17305"
                     /old_locus_tag="SFOMI_3485"
     CDS             12624..12962
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039070.1"
                     /locus_tag="SFOMI_RS17305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SFOMI_3485"
                     /product="TrbC/VirB2 family protein"
                     /protein_id="WP_021245259.1"
                     /transl_table=11
                     /translation="MTAITARLGSQPQWARLLLRIAGIVGLAMLLSLLLSDPAHAQGAD
                     GITSMAENIKTWLTGTFAKTIAVIAVVIVGFMFFTGRASLGLLVTVIVGIFIVFSAQWI
                     VDTITGGA"
     gene            12959..13309
                     /locus_tag="SFOMI_RS17310"
                     /old_locus_tag="SFOMI_3486"
     CDS             12959..13309
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015449339.1"
                     /locus_tag="SFOMI_RS17310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SFOMI_3486"
                     /product="type IV secretion system protein VirB3"
                     /protein_id="WP_021245260.1"
                     /transl_table=11
                     /translation="MKEAAPSNERLREETLFLAVTRPTMWLGVPLEASLPIALAACLTL
                     IVSGNPLYAGAIGGACLAVARLIVRHDANAFRLLWLWTLTKARCRNRGWWGGSSYSPLP
                     VAGMKRKGFARG"
     gene            13302..15713
                     /locus_tag="SFOMI_RS17315"
                     /old_locus_tag="SFOMI_3487"
     CDS             13302..15713
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072616.1"
                     /locus_tag="SFOMI_RS17315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SFOMI_3487"
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="WP_021245261.1"
                     /transl_table=11
                     /translation="MASAAAQLPPRKTPWRVLTREAEPGRYLPYARHVTPDVIALDSGD
                     LMMMFRLEGMAFETADPIHLNDWHEKLNGTWRNIADDRLAIWTHIVRSAVGDYPEGQFR
                     SKFAAELDAKYRARVTAKRMFVNEHYLTLLMRPAVGSADRTGLLLKRIAKAKAAEEEVD
                     PDELARFEEKARDIEKLLRRCSPARLALYEHNGLIFSAPLEVLEQVMMGARSRVPLVRG
                     HLGSALYGERVIFGRETVEIRAHDTSRWQGIFGIREYPALTRPGQMNALLGQDFAFVVS
                     QSFTFMGKARAAERLRRRQNQMASTEDAAASQALDLADAADDLQSNRFVLGEHHFSLAV
                     FADSQRRLADNLSAARAALADAGLVAAREGPALEAAFWAQLPGNFAWRARPAAITSRNF
                     AALAPFHTYPAGRAKGNHWGPAIAMMKTAAQSPYYFNFHVGDLGHTLIIGPSGAGKTVV
                     QNFLMAQLEKTGAQQIFIDKDRGAEIYVRSAGGIYLTLKNGEPTGFAPLRALDYGPRNL
                     VFLGRLIRQLVTPVGGQLGVTQERMIDEGLASLGRLAPEDRSILALRQLLGQRDPEGIG
                     ARLEKWARGGSLGWVFDNEIDALSLEAPFVGFDMTDFLDNPEVRTPLMMYMFHRIDGLL
                     DGRRLVIDIDEFWKALGDDAFRAFAQDGLKTYRKQNAFLVFGTQSPADALRSDISHSIM
                     EQVATKILLPNPYGRETDYVDGLGLTQAEFKLIRHDLNPESRRFLVKQGHDSIVVELDL
                     GGLSDELAVLSGTTETVTLLDAIRAEVGDDPEYWLPLFHQQRRSPSSRKG"
     gene            15717..16433
                     /locus_tag="SFOMI_RS17320"
                     /old_locus_tag="SFOMI_3488"
     CDS             15717..16433
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849915.1"
                     /locus_tag="SFOMI_RS17320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SFOMI_3488"
                     /product="type IV secretion system protein"
                     /protein_id="WP_025546718.1"
                     /transl_table=11
                     /translation="MRKGGFAAIGAIILGTAGPAAAQGIPVYDSSGYLQALATVKNTLS
                     MIDQGKEQIAEAKQLYGSFNQVTDVNGIAPSLSTDAMRHLLPPEARDLGRLMSSENASL
                     GSLGSAATRIRDANRIALPELRPGASAYERASRDNLLRNGDLAARDAAIAESAYGVSAQ
                     RTAGLEELRTSLDTASDAKQVMDIQARVGVENAHIQNDALQLQALRMRQESGRRLQMQR
                     DDEQDLADLSASLGGS"
     gene            16433..16636
                     /locus_tag="SFOMI_RS17325"
     CDS             16433..16636
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF033894.1"
                     /locus_tag="SFOMI_RS17325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="EexN family lipoprotein"
                     /protein_id="WP_076605730.1"
                     /transl_table=11
                     /translation="MLRAIVVSVTLVGLLGCKPPTGKDEYRSNSDLRNQTLRDCANGTH
                     PSSRECVNAQDVKDLDDLKRSL"
     gene            16654..17598
                     /locus_tag="SFOMI_RS17330"
                     /old_locus_tag="SFOMI_3489"
     CDS             16654..17598
                     /GO_process="GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950748.1"
                     /locus_tag="SFOMI_RS17330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SFOMI_3489"
                     /product="type IV secretion system protein"
                     /protein_id="WP_021245263.1"
                     /transl_table=11
                     /translation="MASTLFNDFYMQVDNQLDLFLNERLQNVVEVVRGPLAVGLVIYIA
                     LFGYMVMRGIISEPWGELFYRMVKLCLLYIAATTVAYSEWITTPLFHGMPDALSQALSG
                     RTITSVGAAFDDYFNQVDSIVLIIRAKAATYIEINPMRLVLIALAVCLYALAGLSAAIG
                     FSITIFAKIALAIIIALGPIFIALALFEPTRRFFHGWLGQVFNYIVLMGVIIAITTLIS
                     DLGSIAIAAAESKADVTIGAVLFAVYLFLGTIFFFQAPAIATGIAGGAAAGVGAFAGTA
                     WGTMASPLRQRRIARNSRNLERAARRGGSIEAA"
     misc_feature    complement(17628..17637)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: medium, score: 16.31"
                     /tool="antismash"
     gene            17933..18631
                     /locus_tag="SFOMI_RS17340"
                     /old_locus_tag="SFOMI_3491"
     CDS             17933..18631
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039065.1"
                     /locus_tag="SFOMI_RS17340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SFOMI_3491"
                     /product="virB8 family protein"
                     /protein_id="WP_021245264.1"
                     /transl_table=11
                     /translation="MRVADDDAEAVPTSGLKRYFQEARSWDQDRVRSALRSTRIAWTVA
                     TVTSILAAASIFAVAALAPLKTVVPYVIRVNQTTGAVDVQTALTQRPMRYDEAVTKYFL
                     AQYVRTRESWIPAAAEENFRFVTILSQPAEQQRWARFFSNNNAASPQNAWGKNAVVQAR
                     VRNIAFINDRVANVRFTRIVQTETDTQSSDWIATVTFAYANAPMAEGDRYRNPLGFQVE
                     NYRSDPEVVR"
     gene            18628..19485
                     /gene="virB9"
                     /locus_tag="SFOMI_RS17345"
                     /old_locus_tag="SFOMI_3492"
     CDS             18628..19485
                     /codon_start=1
                     /gene="virB9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684609.1"
                     /locus_tag="SFOMI_RS17345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SFOMI_3492"
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="WP_021245265.1"
                     /transl_table=11
                     /translation="MKTLVAAVFIAFASLSAAHAVEVPRGAASDPRVKFVDYEEAQVYR
                     IVGTFRTATQIVLSEDETIQHVALGDTVSWEVAVAGHILFLKPRERAGPTNLIVTTSRG
                     GTLRSYAFELTARSGPIIGRNGQAYFQVRFRYPRDEVARAERLRAAQIAMQAAALEAQA
                     VRGALDHAVIEGPRNMNYKVQGSSDLQPSEVSDNGQFTVLRFPANREVPAIYLVRPDGS
                     ETLVPFDVRDEFVVVHLVARQLRLRRGGEVLCIYNQAPEPYGVDHGTNTGSSHVERTIS
                     HPQE"
     misc_feature    complement(20149..20163)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 18.2"
                     /tool="antismash"
     misc_feature    20181..20193
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 19.83"
                     /tool="antismash"
ORIGIN
        1 cagcaccaac agggtgagca gcgtcgacga gatgacaccg ccgatgacca ccgtcgccag
       61 cggccgttgc acctcggccc ccgcgccgac gttgaacgcc atcggcacga agccgaggct
      121 cgcaaccagc gcggtcatca gcaccggccg caggcgcgtc agcgcgccct cgcggatcgc
      181 gtcctcaagg ctgcggcctt cggccagcag cgaccggatg aagctcagca tcaccactcc
      241 gttcagcacc gccacgcccg agagcgcgat gaacccgatg cctgccgaga tggagagcgg
      301 cagcccccgg atcaacagcg ccgcaacgcc gccggtgagt gccagcggca ccccggagaa
      361 gacgatcgcg gcgtcccgac ccgagcggaa cagcgcatag agcaggccga agatcaacag
      421 catcgccgcc ggcaccacca gctgcagccg cttggcagcg gagatcagct gttcgaaggt
      481 gccgccgtag ctcacccagt agccggcggg catctcgacc tgcgccccga ccttgctgcg
      541 gagctcctcg acgaacgaac cgaggtcgcg gccgcggaca ttggcggtga cgacgacgcg
      601 gcgcttgccg tcttcgcggc tgacctggtt ggggccgatg accacctcga ccttggcgac
      661 gtcctcgagc ggcacgaagc cgcgtggttc gccgccgccg gctcccagcg ggatgcgcag
      721 ccggccgatc tcgtcgaccc gcgtgcgaag atcttcgggc aggcgaacga taatcgcgaa
      781 gcggcggtcg ccctcgaaaa tctgtccggc gggcgcgccg ccgatcgaca ccgcgaccac
      841 gtcctgcacg tcgccgacat tgagaccgag ccgggcgagc gcggctcggt cgggcgtgat
      901 ctgcagcact ggaaggcctg tgacctgctc gacgctcacg tcctgcgcgc catcgatgcc
      961 cgccacgacc ttctcgatcg cctggccgat ttcgtgcagc cggtcgagat cgtcgccgaa
     1021 caccttgacc gcgacatcgg cgcgcacccc cgacaggagc tcgttgaacc gcatctggat
     1081 gggctgggtg aactcgtagt tgttgccggg gatggtccgg gccgccgcgt gcatctcggt
     1141 gacgagctgg gtccggggct tgcgcgggtc gggccattcg tcgcgaggct ttagcatcac
     1201 gaaggtatcg gcgaccgaag gcggcatcgg gtcggtcgcg acctcggcgg tgccgatctt
     1261 ggcgacgatc cgctcgacct cggggaagcg cttgagccgc gcctccagcg ctctctgcat
     1321 ctcgattgcc tggctgaggc tggtgccggg aatacgcagc gcatggagcg cgatgtcgcc
     1381 ttcatcgaga ttgggcacga actccgatcc catgcggctg gcggcgaggc cggcgagcgc
     1441 gaccagcgcc accgcgccga ccacgaccgc gacgcgcagc cggagcgcca tgtcgagcgc
     1501 gggggcatag gccttgcgcg cgagaaccat gagccggctt tccttctcct cgaccctgcc
     1561 cgtgacgaac agcgcgaccg ccgccggcac gaaggacagc gacaggatca aggcggcgct
     1621 gagcgccatg accacggtga ttgccatggg atggaacatc tttccctcga cgccggtcag
     1681 cgcgaagatc ggcacataga cgagcgcgat gatcagcacg ccgaacaaag acgggcggat
     1741 cacctcggat gtggcggatg ccgcgagatc gaaccgctcc gtgcgcgtca gcagccggcc
     1801 atgcccatgc tgcgcctcgc cgaatcggcg caggcaattc tcgacgatga tgaccgcgcc
     1861 atcgacgatc aggccgaagt ccagcgcacc gaggctcatc aggttccccg acacgcccgc
     1921 ctggagcatc ccggtgatcg tcatcagcat cgtgatcggg atgaccgcgg cggtgatcag
     1981 cgccgcgcgg atattgccga gcagcaggaa cagtacgacg atgacgagca gcgcgccctc
     2041 ggcgaggttc ttctcgaccg tgccgatcgc gcgctcgacg agctcggtgc ggtcgtagat
     2101 cgcctcggct ttgacgccgg ccggcagcgc tttggccgcc tcggcaagcc gttcggctgc
     2161 tgcgcgggca acgatccggc tgttctcgcc cgcgagcatg aacacggtgc cgagcacaac
     2221 ctcgcggccg ttctcggtgg cggcgcccgt gcgcagctcc tctcccaggc ccacgtcggc
     2281 gacatcggcg atgcggatcg gcacgccgcc gcgattggtg acgatgatcg acttcaaatc
     2341 gtcgataccc gacgcctggc cgggcacgcg gatgagatac tgctcgccat agcgctcgac
     2401 ataaccagca ccgacattgg cgttgttgcg tccaagcgcc tgcacgacat cgttcagggt
     2461 gaggccgtag gcggagagcc gctcgggcag cggcgtcaca tgatattgcc gctcgtagcc
     2521 tccaatgcta ttgacctcgg tgacgccggg cgtgttcctg agctgcgggc ggatcaccca
     2581 gtcctggagc gtgcgtagat cctcgggact gtaggcgctg ccgtcgggtt tgcgcgcacc
     2641 cggctgcgcc tccagcgtgt acatgaaaat ctcgcccagg cccgtcgcga tcggccccat
     2701 ctcgggcacc accccttcgg gcaactggtc gcgcgcgttc tgcagccgtt cgttgacgag
     2761 ttgccgcgcg aaatagatgt cggtgccatc ggcgaagacc gcagtcacct gggagagccc
     2821 gtaacgcgag accgagcggg tatattgcag ccccggcaga cccgcaattg cggtctcgac
     2881 cgggaaggtc acgcgctgct cggcctcgag cggcgagaag cccgccgctt cgctgttgat
     2941 ctggacctgg acgttggtga tgtccggagt cgcgtcgatg ggcaggcgct ggaacgccca
     3001 gatgccgatg gcgcagagca gcgcgacggc ggccagtacc gcccagcgga accggatcgc
     3061 gccggcgacg agcctttcca gaatcggtgg gcgttcgaac agcggcggac gggcgggagc
     3121 ctcaatggtc atggctcgcc cccgacttct cgacatccgc gcggatcagg aaggcgccgt
     3181 cggtcacata ttcggtgccg ggttcgagcc cgccgaggat ctcggtccac tcgggcgtgc
     3241 gccgcccgat ctcgagcatc cgcacctcat aggtgttgcc gactttggca tagaccacct
     3301 cgaagtcgcg gaagcgctgg atcgccttgg tcctgaccgc gagcgggaca ttcgccgacg
     3361 cgactgcgaa ctgtccctcg acgcccatgc cgggacggaa catgcgcgcc gccgattccg
     3421 gtaggtggac atgggccatc agcgtctggc tggcgagatc ggcggtcggc aggaccgcct
     3481 cgaccgcggc ctcgatccgc gcctctccgg aaagactgcg cacgcgcacc gtctgcccga
     3541 cacggacgcg ctcggcatcg cgcggataca cgaagaactc ggcgtggagc ttcgccgggt
     3601 cggcgatcac gaacagcgcg cgatcgccgg ttacgtcgcc gacattggcg ttcttttcga
     3661 tgatcgtgcc gctgatcggc gccggcaccg cgtaggtctg gagcgagtgg ctggattcga
     3721 cgcggagcag ggcctgaccc ttgcggacct gctggccgag ctgcccgctc atccagacga
     3781 tctgccccgg cagccgcgcc cggacatcgg ccttgccttc gggcgtgatc tcgatccgtc
     3841 cggccatgtc gatgagctcg gcgatggcga cgggtcctgc ccgttcggac ttcacaccgc
     3901 cggcgcgcgc cgcctcggcg gaaatcgtcg tgcggccctc gtaggaggca taggaccagc
     3961 tatgtgtgcg cccgccctcg accgcgcgca ccttgacgtc gaacgaatgc ggctcatgca
     4021 cgacgccgcc tcccagcaga tagtcctcgc gcggcgtgaa ggcgaagcgg tcgatccggc
     4081 cgccgagccg cgtgagttcg acgccgagtt gcacttcgcc cggcgtcacg ggcttgccgc
     4141 cgcgataggc gtagacgtgg aactcgggtt cgacgccatc ctcgaagatg gtcatctcga
     4201 ccgcgaaatc gccatcgcgc agcattcgtc cgcgatgcgg gccgcgctca tagtcggcgg
     4261 ccttggcggc gggttcttcc ccggtttcgt tggcggcggg gctgccgctg caggcggcca
     4321 gcaaggcgca agcggcgagg atgccgccgc gtacaaggaa ggaggtcttc atcagcggtt
     4381 ctccgcgctg gcgatgaggg gaagatggcg gccggttagc cggtcgagcc gggcgccgat
     4441 gagatggaag cggcgcagca ggtcgatgcg gcgcgagcgc agctcgatca ccgcctggct
     4501 cgcctggctc acctcgagaa aggtgaaggc ggtgccgccg cgccggaagc cgtcgcgcac
     4561 catgaccacc gcacgttcgg cgcccggcag cacttcgcgg tcgagccgaa cgatttcggt
     4621 cgcgagcgcg gcgcgttcgg ccgtgagccg gtcgatctcg cgtcgccgtt cgacccgtgc
     4681 caccgcgatc tcggcttcgg ccgcttgggc atcggcacgt gcgcgctcga cattgccgcg
     4741 attggcggaa cggttgccga gcgggataga gccgccgacc accagcgcga cgtcgttgcc
     4801 ctggccgaaa tgccgcacgc cgacccgcgc ggtcggatcc cccgcattgc cggtctcggc
     4861 aaggcgaacg cgcgcatcag ctgcggcgcg ctcggcgtcg agcaaggcga gatcgggaag
     4921 atcgccctcg gcggcgggag cggcggcgag attggtgaac ggcgcggtgt cgagccgata
     4981 gtccgccgtg ccgccccacc aggcggcgag ataggcgcgg gcgatgcggg cggccgcctg
     5041 ggcctggtcg agcgcgatcc gcgcctgcgc caccgacgtt ttcgcgcgct cggccgcgaa
     5101 cagcggatcg agcgcagcgg cgacccggcg acggacttcg gtttcaaccc gctcggcctc
     5161 ggcgagccgc tgtccggcga ccgccaccgc cgcctcggcg gaaagcgcct cgacccatgc
     5221 cgcctgcacc tgggcgagaa ggtcgagcat ccgtagccgc gtacgcgccg acgtcacgcc
     5281 taccgacgag cgggcggcgc cgatgcgggc ttcgcgcttg ccgccgcgtt cccaggtgcg
     5341 ctcgtaccag gccgtggtct gcgaacggtc gatcggcgaa taggggcctg tgccggcgaa
     5401 atcctcgacg tcgacgccga tcacgtcgcg gggacggaca tcggcctggg caatagcggc
     5461 gttcgccgcc tggagttggg ccgcagtggc cgcgacgacg ggatcgccgc tcgccacccg
     5521 ggccagcgca tcggaaagcg tcaggttttg ggccgaagcc gcctgcgcgt agaggaaaag
     5581 tggaagcacg gcgcagagca ccggcatctt ggcaaggcgc gcgtgggcac gcgcgcgatc
     5641 gaatgaaaac atgaaaggcc tcgcaagacg gcacgacaca tccgcgccga acctgcgacg
     5701 cggggctcag gcgtttgggc cgagatcctt gggaggtcgt tcggggccgc gcgccttcac
     5761 gccgagcggc cgtcgctgcg gctcgaaagt gcccgcgctg gcggccagcg cgagtatcgc
     5821 cgccggcgcc tgatcgggga cgatcaggcc ggagccgcca tcggcatggt ggtgatgacc
     5881 gccggcaaga tgcccgacag gatcgtcgtc gctgtcgtcc ggctgcgggc tgtgatgatc
     5941 gtcggcatga tccagctcga gcgaaagatc gcccaggagc atgtgctcat gggcgatcgg
     6001 cgcgcccggc gcatgctgga ttcggtccat ggagttagac agcgacatcc ccgcaaacac
     6061 gaagacgagc gcgatgcaga gcatcgcggt caaccgcttt acgatggagg tttcgctggt
     6121 ttgcacattg agtcactaac cgtccgcttg ttttcaggca agccaactcg gcatgagcaa
     6181 agcaatcttg gcttttctaa tgaagcgggc gcgctttgct tcgcggattg ccgttgtaac
     6241 agcacgacga cagttagctc tgaaaactcc aagccatcgg caggatctct gccggtggtg
     6301 cagggcggac tggcgtatcc tcacctcata agagcgagtt cgccctgtgc ggcatgtttc
     6361 gtgagactgg tcgcagacac gaggccccgg gcgtcgccga tcaaggtata gacggcgtgg
     6421 cgctgcccgc cgcggtttcg atggtcgacg tgatagccct gataatgacg gcgcatgaga
     6481 tcggcgttca ccaattcgga gacggcgcgg ctgaggttac tcttcccggt ggttcgcatg
     6541 cgtgcgcgca tttcctctgc gatcgttcgt tgcgtttcct gtgcatcgcc ggtgagcatt
     6601 ccacttctgt ccagatccct acgaacgcga tctgccaacg ctgcgctgcg ggatactcga
     6661 tgagacatcg gtgtgagcgc gtcgcagagc cagtcgcgca gcgaaacaag ctcgttgtcc
     6721 cgcttgatcc agggaccggc gctcccgtcg gggccggcga cctgcgcaat caagttgagc
     6781 agcatgaagg catagcgggg acggcttgag acctgcgcca atctctcgag cacggctgcg
     6841 atatcgcagc tgctggaaga ttggagagga gcgccgggat gaaacataac gcgactgaat
     6901 caagcacaga cgaggatcaa tgtgaatccg aaattcgcac cgtggagcgt cctttgtcgc
     6961 ctatgacact ttaccagcag gcaaagtgtc ataggcgatt catccaagca ggtttgttgc
     7021 gaatcgttct caactcaccg tgataatcgc ggagctggcc gcttaacgct ggacgagaag
     7081 ttcttttggc gacgagcttc gaccgtctat ggcgaacagg ataagagact gcggccgcct
     7141 ggacgacctc acgcgtttcg cacaggaatg tgcgcgtgct ggaaacctcg gcgatttgca
     7201 aggcacgatc gatgcggccg tgcgcgaact cggcttccgc tggttcacgc tgctccacaa
     7261 tgtcgatctt cggcacggag gcgggcagcg cctgttcctc accacctatc cgccggcctg
     7321 gctcgaagag gtgctcgagg agcgccacta tatcgaggac cccatccacg cggcctgcgc
     7381 ccgcacgcca tcggggctcg cctgggatcg ggtgggcgac gtgcttgagc tttcgtcgcg
     7441 ccagcgaagc atcctgaaac gcgccaggga tcatgacctt gcagcgggct atagcttgcc
     7501 gatcaggacg ccgggtgagc ctgaggcgat tttcaccgtc gcccgaccgc gtgatgaacc
     7561 gctggatgcg gcggaaattc tcaccgcccg gttgctggga tcggtcgctt atgaccgggc
     7621 gcgcgaactg ctcggcgaag agataaggac cttggcgttc gtttccctca gtccgcgcca
     7681 ggtcgagtgc atcgcgttcg tggcccaggg taagagcgat tgggagattg cccagattct
     7741 cgggcttagc cgcgacaccg tgcatgaata tgttgaatcc gcgcgtcggc gctacggtgt
     7801 gcggcgccga acgcagctgg tgctgcgcgc ggttcgcgac ggtcatctca atatggacgc
     7861 gctcctgtga tctcgctcgg gccgtgatga tcgggcttgg gcaagttcag gacttctgcg
     7921 cggtcgccgc gataatcaag gacagccaag cactggccgg cctgatggcc gaaattacgc
     7981 gtgcgatggg ctttcggtac gtttctctgg tccaccatat cgacctcaag ccagcggtga
     8041 gctctgtcca tatcgtcgat tatccaccag aatgggtgga gcgtttccag gcgcgtcgcc
     8101 tctatgccag tgatccgatc caccgggcca gccatcgcac taatatcggc tttgcctggt
     8161 cggcagtgca atcccttatc acgcttagtg ctgctgatcg gtcgatcctg gcggaggcgc
     8221 acgacgccgg gctgggcgat ggtttcacgg ttcctgccca tattccgggg gaagtgaatg
     8281 ggtcctgctc attcgccatg gggtcaggcg acgtgctcga tcaacgacag ctcccgctcg
     8341 tccagctgat cggcagcttc gcattcgaag ccgcgcgcag gctttcccgt caaaccccag
     8401 ctccatgcga aggcccgtgc ctgactgagc ggcaggccga atgtgtggcg ctggtagcac
     8461 gcgggaaaac cgactgggag attagccaaa tcctcggcat tgggcaggaa acggtgatcc
     8521 agcatgtcaa ggatgcacgc gaccgctacg gcgtgacgaa gcgcacgctg ctggcgatcc
     8581 gcgcattgtt cgatggacag atcagcttcg cagatgtttt cgggcgatga tctccccagg
     8641 acaggggata accgaaagaa cgcgggcctg atcgactgac cgcctcacca aaggagatgg
     8701 catgaatgac tggtgatcgg ccttgctgcc tgatgttgtg gtctttgcta tggcgttggg
     8761 cgaggcggat aggttgcagg ctggattggc atcttagggc attggaccct gtaaaggaga
     8821 ttacccgccg cttgccgctg aacgaccagc ttgaggcccg gttttggccg gtgacctcgt
     8881 ataggagttt ggccgggccg ttcacaccca attgggttcc acctcaccca acggttggag
     8941 aatagccatg tcttatctcg gtgtcgatct acatcagaac tcgattacaa tatgccgccg
     9001 tgaggcggat gggagcgagg tcttcgagac cttgaccctc tctcctgccg acctcgatcg
     9061 cttttgcctg agccttgacg ccgacgacga ggtggcgatc gaggcgaccg gcaatagcgc
     9121 ttggttccgc gaccaggtgc tgccctgcgt gggccgggtg gttgtggtca atcccagcca
     9181 gttcaaggtg atcaggaagt cggtgaagaa gaccgaccgc aacgacgcgg ctgccctcgc
     9241 gctgttcctc gaaaaggaca tgctgcccga gacaagggca aagacccagg ccgaagctga
     9301 gctggcgtcg cttgcccaca cccgcgatct gctggtcaag cagcgcaccc ggctgttgaa
     9361 caagatccat gcgctgcatg tcggcaaggg gctcaagctc aaaaaggaaa gccttggctc
     9421 gaaacggggg ctggcagcca tcgacatggg cctgttctct gcttgcgagc agatcgagat
     9481 cgcggtcctg cgcgaaaccg caaccgcgct gaccgcaagc ctcaagcagc tcgacggcga
     9541 gatcgagaag ctcgcttcgg cgctggcggg ctacgagggg atcgtctcga tcaagggcat
     9601 cggcgctcgc tcggccgccg tgctgctcgc gggcatcggc aacgtccacg actttgaaag
     9661 cgccgacaag cttgccgcct acttcggcat ggtgccgcgt gtcagccagt cgaacgaaac
     9721 cgaccaccgg ggccgcatca ccaagcgcgg caacaaactg gcgcgcacca cgctggtcca
     9781 gtgcagcctc atcgccacgc gctactcgcc atacctcaag gccttctacg accgcatccg
     9841 cgcacggcgc ggcaccggca aggcgatcat cgccaccgcc agaaagctgc tcgccatcat
     9901 ctacgacacc ctcaaaaacg gctgggtgtt cgaagacttc actcagttcc agcgccggga
     9961 aaatccagtc cgcgctggac aatcatcata ggaggctgtc atgcatatcg caaccggcgc
    10021 tgcccgcgcc atggaaaacc gcctgttctg ttcgatgttc gaggaacgca aacgggtatt
    10081 cgtcgatctt ctgcgctggg acgtgccggt ctttgccggt cgttatgaaa tcgaccagtt
    10141 cgacaatgac cgtgccgtct acatcgtgat agccgacgcc gagggcgagc atcttgcctc
    10201 ggcgcggctg cttccgacga ccggtgacca tattctcggc accattttcc ccgagctgtg
    10261 cgaacaagtg cctccccggg gtggtgcgat cctcgagatc acgcgctttt gtcttgcacg
    10321 acgattgcgc gcacgagagc gcctggaggt gcgcaatcgg ctggtgtccg cgctcgtcga
    10381 atatgcgctc ggcaatcata tcgacagtta caccggcgtt gctgagtggt tctggttcca
    10441 acagatcctg agctttggct gggcatgccg tcccctcggc ctgcccgcct caacaagcag
    10501 ccgctgcctt atcgccttac agatcgacat caatggcagc acccgcgagc agctgcaggc
    10561 cagcggcatc tggcgctcgg tcggccttgc cgatcttgcc aaggctgctt gaagagggca
    10621 ccatgacaaa tctctccccc tatcgcccag ctgccgaacc tgcggtcgat gacgatttac
    10681 agcggcacgg cttctgcgtg ttgcgggaca tcgcatccaa gacagaggtc gcggagatcg
    10741 atcaggccct cgatccgcgg tttgccgcca cacccttttg ccagggcggc ttctacggtc
    10801 cgctcaccaa gcgctttggc ggccttttga aacgcgtacc cactgtcgag cgactcgtgc
    10861 gccatcctgc gatcatgcgc ctggtgcggt cggttctcga cccctggtgc gataccatcc
    10921 agctcaacct cacgcaggcg ctcgaactgc atccaggagc gccggcgcag tttcctcaca
    10981 gggaccagga tatgtggcgc ggcccggccg gcgagctcga atatctcgtc aacgtgatgt
    11041 ggccgatttc tccgttcaag gctgaaaacg gcgccacatt gatctggtcc gggagccata
    11101 atgccggctc catggtcaag gacgcgcagc ccatggcggt tgaggcggag ccgggctcgg
    11161 cgatcctctt cctcggttct acgctgcatg gtgccggcgc caacatgacc ggcgcgatcc
    11221 ggcgtggcat catcgtcagc tattgcctgg gctggctgaa gccctacgaa aataaatggc
    11281 tgacctatcc gcccaatgcg gcccgtgcgt tcgatccaga ccttgccgcg ctggtcggct
    11341 accagcagca ccgtcccaat ctcggcaatt atgatggcca gtgtccgtcc gtcctgcttc
    11401 ggggcgaacc gccggaatat ctggctgcca ccgatgctct gcgacccgat caggaaagtg
    11461 cgctggccga atttctggac cagcaacgca cgagcctact ccccccggct ggggggattg
    11521 ccgaaatcgg ggccgggcgg aacaaaagag aggtgaaatc caacggatag ctgacgccaa
    11581 ttcccgacgc gggaggtgtc tctcacgggg gaatgacccg atgcaacgcg tgactctcag
    11641 cctggcggtg ctcgccatgg gcaccgccct tcaaccgact cccgcgcgag cgaatgactt
    11701 gggctgccag gttctcctgt gcctttctaa tcctggtggc gcgacgcaat atgcccagtg
    11761 cgtcgcgccc atgaccaagt tgtggcagcg cttggccacc ggcggctctt ttcccggctg
    11821 tagcggcggc ggtgttgctc gtaccaaggt ccacgatcgc gattccgcaa cgcggcgccg
    11881 ggttgtcatg actttcagcg atggacgccg cgagacctac tcgctggccc atatcgagcg
    11941 gctgcaggat ctcgctcgat gatcctcgac gtcgccagtc ttctcgccct tgcaggctca
    12001 tgcgcgccaa gcgtggcgcc tgagacgctg atctcgatcg ttcacacgga aagccgattc
    12061 aacacgctgg cgatcggggt caacagtccg ggtgcgcgca agccggtgcc ggcgacgagg
    12121 gcacaggcga ttgccgccgc gcgtcgcctc attcgccaag gctacaacat cgatttgggc
    12181 ttggggcaga tcaacagcgc caacctcgga tggctgggtc tgtcggtgga ggatgccttc
    12241 gagccgtgcc gcaaccttgc cgccgccgcg cgggtgttga cgaccaacta tcagtcggtg
    12301 gcgcactccg cccagagttc ggaccatgcg atcgcaacag cgctctcgct ctacaatact
    12361 ggcgatcggc ggcgcggctt tcgcaatggt tatgtcgccc gggtctatgc cagcgcgtca
    12421 accgtcattc ccctgatccg cggcaggcac ccagcgtcgg tgatgaccgc gcctgtccgg
    12481 cttgccgatg cggtgatcgc caaaccgcaa cccactgcgc tcgctgcgac gcaggccgct
    12541 ggttggaaca tcgccgcaga tgcccggcaa gcctcgctca tggtcttcgg ggacgggaca
    12601 acttctcaga aaggacagca tccatgacag cgatcaccgc ccgcctgggc tcccagcccc
    12661 aatgggcgcg tcttctgctg cgcatcgccg gcatcgtcgg cctggcgatg cttctctcgt
    12721 tgctgctgag tgatcctgct catgcgcaag gtgcggacgg catcacctcc atggccgaga
    12781 acatcaagac ctggctaacg ggaacctttg ccaagacgat tgcggtgatc gccgtcgtca
    12841 tcgtcggctt catgttcttc accggacggg cgagcctcgg ccttttggtg acggtcatcg
    12901 tcggcatttt catcgtcttc agcgcgcagt ggatcgtcga taccatcacc ggtggcgcgt
    12961 gaaggaggcc gcgccctcga acgagcggct gcgcgaggaa acgcttttcc ttgcggtgac
    13021 ccggccgacg atgtggctgg gcgtgccgct cgaagcatcc ttgccgattg cgcttgctgc
    13081 ctgtctcacc ctgatcgtca gcggcaatcc gctttacgcg ggggcgatcg gcggcgcgtg
    13141 cctcgcggtg gcgcgcctca tcgtgcgcca cgacgccaat gcctttcgcc tgctatggtt
    13201 atggactttg accaaggcgc gctgccggaa ccgcggctgg tggggcggca gttcctattc
    13261 gccgctgccg gtcgcgggga tgaagcgcaa gggtttcgcg cgtggctagc gccgccgccc
    13321 agctgccgcc gcgcaagacg ccttggcggg ttctgacccg agaagcggag cccgggcgct
    13381 atcttcccta tgcccgccat gtgacgcccg atgtgattgc gcttgatagc ggcgatctca
    13441 tgatgatgtt ccgcctcgaa ggcatggctt tcgagacggc tgatccgatc cacctcaatg
    13501 attggcacga gaagctcaac ggcacttggc gcaacatcgc cgacgatcgg cttgcgatct
    13561 ggacccacat cgtccgctca gccgtagggg actatcccga agggcagttc cgctcaaagt
    13621 tcgccgccga gctcgacgct aaatatcggg cgcgcgtcac cgccaagcgg atgttcgtca
    13681 atgagcatta tctgacgctt ctcatgcgtc cggcagtcgg atcggccgac cggaccgggc
    13741 tgctgctcaa gcggatcgcg aaagccaagg ccgcagagga ggaggtcgat cccgatgaac
    13801 tggcgcgctt cgaggagaag gcgcgcgaca tcgagaagct gctccgccgc tgcagtccgg
    13861 cgcgccttgc tctctacgag cataatggcc tgatcttctc cgccccgctt gaggtgctcg
    13921 agcaagtgat gatgggtgcg cgatccaggg ttccgctcgt ccgcgggcat ctgggatccg
    13981 cgctttacgg cgagcgcgtg atcttcggac gtgagacggt cgagatccgt gcccatgaca
    14041 cgagccgctg gcagggaatc ttcggcatca gggaatatcc cgcgctgacg cgcccgggac
    14101 agatgaatgc cttgctcggc caggatttcg ccttcgtcgt ctcgcaatcc ttcaccttca
    14161 tgggcaaagc gcgggcagcc gaacgtctgc gccgccggca aaaccagatg gcctcgaccg
    14221 aggatgccgc agcgagccag gcgctcgatc tcgccgatgc agccgacgat ctgcaaagca
    14281 accggttcgt gctcggggag catcatttct cgctggcggt cttcgccgac agccaaaggc
    14341 gacttgccga caatctctcg gccgcgcgcg cggcactggc cgatgctggt ttggtggcgg
    14401 cgcgcgaagg accagcgctc gaagcagcgt tttgggcgca gctgcccgga aatttcgcat
    14461 ggcgggcgcg gccagcggcg atcacctcgc gcaattttgc tgccttggcg ccgttccata
    14521 cctatcccgc aggccgggcc aaggggaacc attgggggcc tgcgatcgcc atgatgaaga
    14581 cggccgcgca atcgccctat tatttcaact tccatgttgg cgacttgggt cacacactga
    14641 tcatcggtcc ttccggcgcg ggcaagaccg tcgtccagaa ttttctgatg gcgcagctcg
    14701 agaagacggg cgcgcagcag atcttcatcg acaaggaccg cggcgcggag atctatgtcc
    14761 gatccgcggg cgggatctat ctgacgctga agaatggtga gcctacgggt ttcgctccgc
    14821 tgcgggcact cgactatggt ccgcgcaatc ttgtcttcct ggggcgcctg atccggcagc
    14881 tggtgacgcc ggttggaggc caactcggcg tcacgcagga gcgcatgatc gatgaggggc
    14941 tggcctcgct cggacgcctt gcgccggagg accgctccat tctcgcgctt cgccagctgc
    15001 tcggtcagcg cgacccggaa ggcatcggtg cccggctgga gaaatgggcg cggggcggtt
    15061 cgctcggctg ggtgttcgac aatgagatcg acgctttgtc gctggaggcg ccgttcgtcg
    15121 gcttcgacat gaccgatttt ctcgacaacc ccgaagtgcg cacgccgctc atgatgtaca
    15181 tgttccaccg gatcgacggt ctcctcgacg gccggcggct tgtgatcgat atcgacgaat
    15241 tctggaaagc cttgggggac gatgcgttcc gcgccttcgc gcaggacggc ctcaagacct
    15301 accgcaagca gaatgccttt cttgtcttcg gcacgcaaag ccctgcggat gcgctgcgtt
    15361 cggacatttc gcacagcatc atggagcagg ttgcgaccaa gatactgctt cccaacccct
    15421 atggccgcga aacggactat gtcgatggac tgggtctgac ccaggcggaa ttcaagctca
    15481 tccgccacga tctgaatccg gaatcccgcc ggttcctggt caagcaaggc catgactcaa
    15541 tcgtcgtcga gctcgatctt ggcggtctct ccgacgagct ggcagttctc tcgggcacca
    15601 cggaaacggt gacgctgctc gatgccatcc gcgccgaggt cggcgacgat cccgaatact
    15661 ggctgcccct ctttcaccag cagcgtcgat ctccatcgag caggaaagga tgagctatgc
    15721 gtaaaggcgg attcgcagcg atcggtgcga ttatcctggg aacggcgggt ccggccgccg
    15781 cccagggcat accggtctat gacagctccg gctatttgca ggcgctcgcc acggtcaaga
    15841 acaccctgtc catgatcgac cagggcaagg agcagatcgc cgaggccaag cagctctacg
    15901 gtagcttcaa tcaggtcacc gacgtcaatg gcattgcccc gtcgctatcg accgatgcca
    15961 tgcgccatct tctgccgcct gaggcccgcg acctcggcag gctgatgtcg tcggaaaatg
    16021 ccagcctggg atcgcttggc agtgccgcga cccgcattcg cgatgccaac cgcattgcac
    16081 tgccggagct gcggcctggc gcatccgctt atgaacgggc gtcgcgcgac aacctgttgc
    16141 gaaacggcga tctggccgcc cgcgatgctg cgatagcgga gtcggcctac ggcgtctcgg
    16201 cccagcgcac cgcaggcttg gaagaactgc gcacctcgct cgatacggct tcggacgcca
    16261 aacaggtcat ggacatccag gcaagggtag gggtcgaaaa cgcccatatc caaaatgatg
    16321 ccctgcagtt gcaggcgctg cggatgcggc aggagtcagg acgtcgtctt cagatgcagc
    16381 gcgacgatga gcaggattta gccgacctat cggcaagttt gggaggctcg taatgctccg
    16441 ggccattgtc gtaagcgtta ctttggttgg cttgctcggg tgcaaaccgc cgaccggaaa
    16501 agatgagtat cgcagtaact ccgatctgcg gaatcaaacg ctcagggatt gcgcgaacgg
    16561 aacccatcca agttcccgcg agtgcgtcaa tgctcaggac gttaaagatc tcgatgacct
    16621 gaagcggtca ctttgacggc gagctgaccc gcaatggcct ctacactgtt caacgatttc
    16681 tatatgcagg tcgacaatca gttagacctg ttcctcaacg agcgtcttca aaacgtcgtt
    16741 gaggttgttc gcgggccgct cgccgttggg cttgttattt atatcgcttt gttcggctac
    16801 atggtgatgc gcggcattat tagcgaaccc tggggcgaac tattctaccg aatggtcaag
    16861 ctctgcctgc tgtatattgc cgcaacgacg gtcgcctatt ccgagtggat caccacgcct
    16921 ctttttcatg ggatgccgga cgcactctcg caggcactct ccgggcggac catcaccagc
    16981 gtgggcgctg catttgatga ctacttcaat caagtcgaca gcatagttct cataattcgc
    17041 gccaaagctg cgacgtatat tgaaattaat ccgatgcgtt tggtgctgat cgctctagcc
    17101 gtttgcctct acgcgctggc aggattgtct gccgcgatcg gtttttcaat tactattttt
    17161 gccaaaatag cgctggccat tattatagct ctcgggccaa ttttcattgc acttgcattg
    17221 ttcgaaccta cgcgccgctt ctttcatgga tggctcgggc aggtcttcaa ctacatagtc
    17281 ctcatgggag tgatcatcgc gatcacgacg ctcatttctg atttgggcag catcgcaatc
    17341 gcggctgcag aatcaaaagc tgatgtgaca atcggtgcgg ttcttttcgc cgtttatctc
    17401 ttcctcggga ccattttctt tttccaggct ccggccattg ccaccggcat tgcgggcggc
    17461 gccgctgcgg gggttggtgc cttcgccggg accgcctggg ggacgatggc atctcccctc
    17521 cgacaacgcc gcattgcgcg caacagccgc aatctggagc gcgccgctcg tcggggcggc
    17581 tccatcgaag cggcatagtg agacatcacc atgaccttgc cctgtcctca aaaatcgacg
    17641 ttcaccatcc gccaggttac tttctgcctg atcggcagcc ttccactgct gggcctcgcg
    17701 gcatgcgcgt ccggacccaa gaagctggcc gtttgcaacg ggcaccatct gcgtgacgtg
    17761 aacatctatg ggagcgttct gcccggatcg ccggtgcggg cgactacagc gccgcgccct
    17821 gcgccgccca ttcctccctt gtctcatccg caggtgggcg atccaccgcc tccgcccgca
    17881 gtgccagcgc ctccggacga caaggtttcc tttgcgccaa aggacattcg ttatgcgagt
    17941 tgctgacgat gacgcggagg cggtgcctac ttccgggctc aaacgctatt tccaggaagc
    18001 gcgaagctgg gaccaggaca gggttcgatc ggcattgcga tcgacgcgta ttgcttggac
    18061 cgtggcgacg gtaacctcga tccttgcggc cgcatccatt tttgctgtcg ccgcgctggc
    18121 cccgctcaag accgtcgtgc cctatgtcat tcgggtcaat cagactacgg gcgcggtgga
    18181 tgtccagacg gcgctgaccc agcggccgat gcgctatgat gaagcggtca ccaaatactt
    18241 tctcgcgcaa tatgtccgga cgcgtgaaag ctggataccg gcggctgccg aggagaattt
    18301 ccgcttcgtg acaatcctgt cgcagccggc ggagcagcag cgctgggcac gcttcttcag
    18361 caacaacaat gctgccagtc cccagaacgc ctgggggaaa aacgcagtcg tccaggcgcg
    18421 ggtgcgcaac atcgccttca tcaatgatcg ggttgccaat gtgcgattca cacggatcgt
    18481 ccagaccgag acggatacgc agagcagcga ttggatcgcc acggtcactt tcgcctatgc
    18541 caacgcgccg atggccgaag gtgatcgcta tcggaacccg ctcggcttcc aggtcgagaa
    18601 ttaccgctcc gacccggagg tagtccgatg aaaacattgg tcgctgcggt tttcatcgcg
    18661 tttgcatccc tttctgctgc ccatgcggtt gaggtcccgc gtggcgctgc gtctgaccca
    18721 cgcgtcaagt tcgtcgatta tgaggaagcg caggtctatc gcatcgtcgg gaccttccgg
    18781 acagcaaccc agattgtcct cagcgaggac gaaacgattc agcacgtagc gctgggcgac
    18841 accgtctcct gggaggtggc ggtggccggc cacatcttgt tcctcaagcc gcgcgaacgc
    18901 gctggaccga ccaaccttat cgtcaccacg tctcgcggcg gaaccttaag aagctatgcc
    18961 tttgaactca cggctcgtag tggtccgatc atcggccgca acggccaggc ctatttccag
    19021 gtccgcttcc gctatccgcg ggacgaggtg gcgcgggccg aacgcctgag agcggcacag
    19081 atcgcgatgc aagcggcggc gctggaggcc caggctgttc gcggtgccct cgaccatgcg
    19141 gtgatcgagg gtccgcgcaa catgaattat aaggtgcagg gttcgagcga tttgcagccg
    19201 tcggaggtct ccgacaatgg ccaattcacg gttttgcggt ttccggcgaa ccgggaagtt
    19261 cctgccattt atctggtgag gccagacggg tccgaaaccc tggttccctt cgacgttcgc
    19321 gatgaattcg tcgtcgttca tttggtggcg cgccaactgc gcctgcgccg gggcggcgag
    19381 gtgctgtgca tctataacca ggcgcccgaa ccttatggcg tcgatcatgg aacgaatacc
    19441 ggatcgtctc acgtcgagcg caccatctcc catccgcagg aatgaaccat ggccgatgaa
    19501 ccgtcccctg tcgtgcgccc gctgcccggt gccgatccaa cgcccgaaag gcacgaaatt
    19561 cttcacgagc gtggaatcga gccgatcggc ggcacgacgc ccgcgcggcg caacaccgcg
    19621 ctgatcttcg ccggcacggc aatggtcctg ggcattctct gggtgaattc cggcacgggc
    19681 cagcaatcct cgtctcgtga cctcaccgcg ccggcaggtg cagcccggga acgacccgat
    19741 atcgtcgctc gggaaacagt cgattatgcc gcggtcgccc ctcaacgccg accgctcggg
    19801 gctgcccgcg cggaccccaa cgcgccggtg ctcaatcctg ccgctggcgt tcccggacct
    19861 gaaggccaaa ttgtgcccgc catgcagccg ggcgcggcac cgagcgggac cagtgccgct
    19921 cggccaacgc ttgccgagca ggcgcggcgg tccacgctga tcgcctatgg aggtcgcgat
    19981 cttggccgcg aggccggcgc gcccacagct gcaggggctg ccggggaagc gcccgacaac
    20041 gctgccggag cgccagagag cggcgagggc agggcgccca acgcgctcga tcttttgcgg
    20101 caaagctcct cgaccgggga agcgcgggcc tcgatgctgc cgaaccgcaa ttttctgatt
    20161 acggcgggca cattgatccc ctgcattctg caaaccgcca tcaattccgc gcagcccggt
    20221 tacacgtcct gcctgatccc gcgagacgtc tattccgaga atggccgggt cgtactcatg
    20281 gaaaagggca cgcgcgttct cggcgaatat cgcggcggca tccagcaagg gcaaaatcgc
    20341 ctgttcgtcc tctggacgcg agcggtaacg ccccagggcg tgcggatcga tcttgcctcg
    20401 cctggctccg atgcactcgg ccgagccggg cttgcaggcg cggtcgacag cttcttctgg
    20461 gcgcgttttg gcggcgccct gctcctctca ctcgtcgatg atgccgccta tatcgccgga
    20521 caggccgcct cgagcggcaa tggcaatttc aacaatgtga cccgggcgcc cagcgaaggc
    20581 gcggcgattg ccttgcagaa caatatcaat at
//
