LOCUS       NZ_BEWI01000031        10414 bp    DNA     linear   CON 12-AUG-2024
DEFINITION  Sphingobium fuliginis strain OMI SOMI031, whole genome shotgun
            sequence.
ACCESSION   NZ_BEWI01000031
VERSION     NZ_BEWI01000031.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Sphingobium fuliginis
  ORGANISM  Sphingobium fuliginis
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Sphingobium.
REFERENCE   1
  AUTHORS   Ogata,Y., Toyama,T., Yu,N., Wang,X., Sei,K. and Ike,M.
  TITLE     Occurrence of 4-tert-butylphenol (4-t-BP) biodegradation in an
            aquatic sample caused by the presence of Spirodela polyrrhiza and
            isolation of a 4-t-BP-utilizing bacterium
  JOURNAL   Biodegradation 24 (2), 191-202 (2013)
   PUBMED   22777343
REFERENCE   2
  AUTHORS   Ogata,Y., Goda,S., Toyama,T., Sei,K. and Ike,M.
  TITLE     The 4-tert-butylphenol-utilizing bacterium Sphingobium fuliginis OMI
            can degrade bisphenols via phenolic ring hydroxylation and
            meta-cleavage pathway
  JOURNAL   Environ Sci Technol 47 (2), 1017-1023 (2013)
   PUBMED   23215053
REFERENCE   3
  AUTHORS   Takada,K., Shiba,T., Soda,S., Inoue,D., Miyake,M., Eguchi,M. and
            Ike,M.
  TITLE     Bioaugmenting a lab-scale membrane bioreactor with Sphingobium
            fuliginis OMI to degrade 4-tert-butylphenol
  JOURNAL   Unpublished
REFERENCE   4  (bases 1 to 1406148)
  AUTHORS   Kuroda,M., Ogata,Y., Inoude,D., Sei,K. and Ike,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-2017) Contact:Masashi Kuroda Osaka University,
            Graduate School of Engineering; Yamadaoka 2-1, Suita, Osaka
            565-0871, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Celera Assembler v. 5.3
            Genome Coverage                   :: 20x
            Sequencing Technology             :: Roche GS FLX Titanium Chemistry
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002723855.1-RS_2024_08_12
            Annotation Date                   :: 08/12/2024 02:52:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,410
            CDSs (total)                      :: 5,341
            Genes (coding)                    :: 5,170
            CDSs (with protein)               :: 5,170
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 171
            CDSs (without protein)            :: 171
            Pseudo Genes (ambiguous residues) :: 0 of 171
            Pseudo Genes (frameshifted)       :: 49 of 171
            Pseudo Genes (incomplete)         :: 133 of 171
            Pseudo Genes (internal stop)      :: 8 of 171
            Pseudo Genes (multiple problems)  :: 15 of 171
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:08:54
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 40075
            Orig. end                         :: 50489
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BEWI01000031.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10414
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10414
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10414
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5414](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5414
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(1363..2280)
                     /locus_tag="SFOMI_RS06065"
                     /old_locus_tag="SFOMI_1191"
     CDS             complement(1363..2280)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820596.1"
                     /locus_tag="SFOMI_RS06065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SFOMI_1191"
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="WP_048574817.1"
                     /transl_table=11
                     /translation="MRAAAWKTIVKRIWVNSGRHNLGLLSAGVAFYVFLSFVPLLAAVI
                     MIYGLAADPATVARHMRTILDVVPADAARLIQEQLTQLTTSAQDKKGVGLLIALLVSIF
                     GASRASGAMIGSLNIIYERQDRRSFLKNTFLASALAGAAVLVGIIGLAAASMLSFAKGL
                     VAGIGPVGATAVQALTWLIAASLCSFAIGGMYRFAPDRPDVRWRWFSLGSCLATLLWLA
                     ATLGFGLYASRFGNYDATYGSLGAVVVLLMWLFVSAYAVLIGGLINAEIERQAPCDTSA
                     EPTEAGGQRMGAARALFQQQAHAR"
     misc_feature    2250..2264
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 19.36"
                     /tool="antismash"
     gene            complement(2409..2897)
                     /locus_tag="SFOMI_RS06070"
                     /old_locus_tag="SFOMI_1192"
     CDS             complement(2409..2897)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1135: MarR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006964691.1"
                     /locus_tag="SFOMI_RS06070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SFOMI_1192"
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_172668358.1"
                     /transl_table=11
                     /translation="MSSEVAVLTLRALRRVLRATEIGSRRLAATTGLTPSQWLVLREIG
                     SRDSATPSAIATALKFSQATITAIVDRLQQLGFVQRQRSEKDKRQFILRPLPPGVEALA
                     EAPDLLQTTFTERFAKLPQWEQAMILAAVERLAMLMDAQNIDAAPLLDSGLIDRSEPS"
     misc_feature    complement(2927..2941)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.21"
                     /tool="antismash"
     misc_feature    complement(3085..3100)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 18.98"
                     /tool="antismash"
     gene            3179..3652
                     /gene="ectA"
                     /locus_tag="SFOMI_RS06075"
     CDS             3179..3652
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831864.1"
                     /locus_tag="SFOMI_RS06075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_048574818.1"
                     /transl_table=11
                     /translation="MRLPTAVDGPLVTKLVASCPPLDVNSAYCNLLQCTHFAQSCIIAE
                     KAGQLMGWVSGYRPPSEPESFFVWQVAVAPTARGQGLAGHMIEALLARPSSAEVTHLIT
                     TVTNDNQASWALFEGLANRWRTRLERSPFFHQQTHFAGAHATEWLARIGPLPR"
     gene            3686..4990
                     /gene="ectB"
                     /locus_tag="SFOMI_RS06080"
                     /old_locus_tag="SFOMI_1194"
     CDS             3686..4990
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (35-367). E-value: 6.8e-75.
                     Score: 244.1. Matches aSDomain:
                     nrpspksdomains_SFOMI_RS06080_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820592.1"
                     /locus_tag="SFOMI_RS06080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SFOMI_1194"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_048574819.1"
                     /sec_met_domain="Aminotran_3 (E-value: 2.1e-79, bitscore:
                     263.4, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MITLPRPTGRKPDTAIYERRESAVRSYARVMPRQFNRAENVWMHD
                     NQGGRYLDFLSGCSTLNYGHNHPVLKQALLDYIVADGITHGLDLHTDAKAEFLETLENL
                     ILKPRRLDYRAMFTGPTGTNAVEAAIKLARKVTGRELVIAFTNGFHGMTLGALACTGNA
                     AKRGGAGVPLAHVAHEPYDGYHGPEVDTAELLERRLADPSSGLDAPAAILVETVQGEGG
                     LNAASPEWLRRINTMAKRHGALMIVDDIQAGCGRTGSFFSFEGMGFTPDIVTMAKSLSG
                     MGLPFALTLFRPELDQWAPGEHNGTFRGNNHAFVTATAALRHFWSDRRFEQDIARRGAL
                     IAQRLEAMAAQHGLSTRGRGMMRGIDVGSGDIAAAVTTACFERGLIIETSGAHDEIVKI
                     LSPLVIDDAELSAGIDILETCIGEALGAPYGVAAE"
     aSDomain        3791..4786
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SFOMI_RS06080_Aminotran_3.1"
                     /evalue="6.80E-75"
                     /label="SFOMI_RS06080_Aminotran_3.1"
                     /locus_tag="SFOMI_RS06080"
                     /protein_end="367"
                     /protein_start="35"
                     /score="244.1"
                     /tool="antismash"
                     /translation="NRAENVWMHDNQGGRYLDFLSGCSTLNYGHNHPVLKQALLDYIVA
                     DGITHGLDLHTDAKAEFLETLENLILKPRRLDYRAMFTGPTGTNAVEAAIKLARKVTGR
                     ELVIAFTNGFHGMTLGALACTGNAAKRGGAGVPLAHVAHEPYDGYHGPEVDTAELLERR
                     LADPSSGLDAPAAILVETVQGEGGLNAASPEWLRRINTMAKRHGALMIVDDIQAGCGRT
                     GSFFSFEGMGFTPDIVTMAKSLSGMGLPFALTLFRPELDQWAPGEHNGTFRGNNHAFVT
                     ATAALRHFWSDRRFEQDIARRGALIAQRLEAMAAQHGLSTRGRGMMRGIDV"
     gene            5001..5414
                     /locus_tag="SFOMI_RS06085"
                     /old_locus_tag="SFOMI_1195"
     CDS             5001..5414
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013022.1"
                     /locus_tag="SFOMI_RS06085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SFOMI_1195"
                     /product="ectoine synthase"
                     /protein_id="WP_048574820.1"
                     /sec_met_domain="ectoine_synt (E-value: 2.9e-49, bitscore:
                     162.6, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRKLQDIRKSDRNVKSQQWESARLLLKDDNMGFSFHITTMYAG
                     EEIHMHYQNHLEAVLVLKGNGTIEDLGTGITHQLASGVMYALNAHDRHIVRPTTDILCA
                     CVFNPPVTGREVHDENGAYPAEADMIRAAALSN"
     gene            5439..6374
                     /gene="thpD"
                     /locus_tag="SFOMI_RS06090"
                     /old_locus_tag="SFOMI_1196"
     CDS             5439..6374
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042345.1"
                     /locus_tag="SFOMI_RS06090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SFOMI_1196"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_099185634.1"
                     /sec_met_domain="PhyH (E-value: 1.3e-30, bitscore: 103.6,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQDIYPSRHAKMAEFLPRLDPVVHSEWSKDAPIGRDEAAQFERDG
                     YLVLENLFSDKEIAFLQSEAGQLLSDPDALEGETVISEPDSREIRSIFRIHAQSPVIAR
                     LAADSRLAGVARFLLGDEVYIHQSRLNYKPGFQGKEFYWHSDFETWHVEDGMPRMRALS
                     MSVLLAENMAHNGPLMLIPGSHRSFLTCVGQTPEDHYRMSLKRQEYGVPDEDSLAELAH
                     RHGIVAPTGPPGSVVIFDCNIMHGSNGNITPFPRANAFLVYNALSNRLCAPFGVEKPRP
                     QFIAARGELQPIVPVSGSMTERTGMPGQAA"
     gene            6381..7628
                     /locus_tag="SFOMI_RS06095"
                     /old_locus_tag="SFOMI_1197"
     CDS             6381..7628
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820588.1"
                     /locus_tag="SFOMI_RS06095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SFOMI_1197"
                     /product="MFS transporter"
                     /protein_id="WP_044663539.1"
                     /transl_table=11
                     /translation="MFLAIKPVRSILLSIFMLMAGSGFLATLISLRLNRAGSGTMTIGI
                     VATAYFGGLIIGSLRAGGVVRRVGHIRAFAAFVALLSASTLSYVLLSHPLFWMLLRLVD
                     GMCVAGVFVCLESWLNDRAEPQMRGSVLAAYMVALYSGQAIGQLLLGASGALPAIPFQL
                     ASILISLAIIPLCLTRSAAPAPVEASAFSIRSLLAASPLGAWGAVATGLMLGAFYGLAA
                     VHVRRLGLDLPETARFMMFVILGGVALQWPLGRLSDRHDRRQVIIGSFAAAALVSMALS
                     LGQDGLWLTGLGALFGGLSFALYPLCVAHCNDRLLETERVAASGRLVLLYSLGAALGPL
                     LAAGAMTAFGTGGLFLFIALCAGLAMTVGLWRQRASEPVPVRYQQDFQVLPRTTPMASL
                     LDPNSADETLTQGQTT"
     assembly_gap    7802..7821
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     misc_feature    8624..8637
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.94"
                     /tool="antismash"
     misc_feature    complement(9741..9755)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.99"
                     /tool="antismash"
ORIGIN
        1 gtgtccggga ccggtctcaa agcggcggga aaggccgtga tagaggcgct ccttgctgat
       61 cagttgagcg gcgaagtcgg cgatgagcgc ggtcgccaga agcggcatca tcaggccgcg
      121 gctcgccgtc gtttccgaaa tgatgatgac ggccgtgagc ggcgcccgca cgacgcccgt
      181 gaaataggcg accatcccca gcaggacgat cgccccggca ggataggccg gaaacatgct
      241 gcggagcacg tcgccgatcc ccgctcctac ggacagggac ggcgcgaaaa tacctcccgg
      301 gatcccggaa accgcagtgg cgagtgtcgc cgcgaacttc gccgccccga accatatcgg
      361 cacgtctgag cctgcgatga cagcatgagc ggtctgatag ccggtccccc aggtgaggcc
      421 tgtggcggcg cccaggagcg ccactaccag tccgcatcct gccgcgaacc gcaccggtct
      481 ttcccgcatc cagcgtatcg gcgtccttgt cgagcgtccc atgcccagca tgaagcgcga
      541 gaaacaggct cccgccaggc cgccaatcac gccggcgacc ggagcggcca caagcgcctc
      601 atggacattg agcgtctgcc gcatgacgcc aaaatagaca tagtcgccag caagccccaa
      661 actcaccatg cccgcgatga gtacagcggc catcaccagc agggccatgc gctgctcata
      721 ggccgccgca agctcctcga tggcaaaggc gacccccgcc agaggcgtat tgaacgcggc
      781 ggccacgcct gccgcgccgc ccgctatata gaccgatgcc cgcaacggaa ccgacatcag
      841 ccgatgcgca taggccatga tcgttgcaga aatctggacg gtcggaccct cgcgcccgac
      901 cgaggctccc agcagcagcc ccgcaacagt catgaccagc ttcacgatgg cggtccgtgc
      961 cgaaaccagc cggttgagcc catgctcggg atcacgagag gcggccatca cttgtggaat
     1021 gcccgacccg cttgccgcag gcgcaagctt gcgggttgtc cagacgatag cggcaaagcc
     1081 caatggcgtc agcaacagat gtgcggccgg ccaccgcctt gccagaccga caaacatttc
     1141 gctcgcctcg tccgccagac gcgcgaacag caaggcggca aggccggtga ggacagcacc
     1201 gcccaaaatg gcgatgcggc ggcgccagat cagggcctcc gggccatgcc gccgcaacaa
     1261 tatccgtaaa tgacgcagtg aaaggctggt tcctggcatc gttttccttc ggcaagtgtg
     1321 ccgccaagcg tcgcggtcag tcagatgatg ggcgcatgcg cattaacggg catgcgcctg
     1381 ttgctggaac agcgcgcggg cggcgcccat cctctgccct cctgcttctg tgggttccgc
     1441 gctcgtgtcg catggagcct gccgctctat ctcggcattg atcaggccac cgatcaatac
     1501 ggcataagcg gaaacgaaca gccacatgag gagcacgacc accgcgccga gcgatccgta
     1561 ggtcgcgtcg taattgccga accggctcgc ataaagtcca aagccgagcg tcgcggcgag
     1621 ccagagcaaa gttgcaaggc aggagccaag gctgaaccat cgccatcgga catcgggcct
     1681 gtcgggcgcg aaccgataca tgccgccgat ggcgaagctg cacagggatg cagcgatgag
     1741 ccaggtcagc gcctgcacag ccgttgcgcc cactggcccg atcccggcga ccagcccttt
     1801 ggcgaagctg agcattgagg cggccgccag gccgatgatg cccacaagga ccgctgcacc
     1861 ggcaagcgca ctcgcaagaa aggtgttttt caggaacgaa cgccggtcct gccgctcata
     1921 gatgatgttg agcgatccga tcatggcgcc cgaagcccgg gacgcgccga agatcgagac
     1981 cagcagcgcg atcagcagtc caactccctt cttatcctgg gcgctcgtcg taagctgcgt
     2041 gagctgctcc tggatgagcc gggcggcgtc ggcgggcacg acgtcgagaa tggtgcgcat
     2101 atgcctggcg accgtcgcgg gatcggcagc aaggccgtag atcatgatga ccgccgccag
     2161 aagcggcacg aacgacagga agacgtagaa ggcgacaccg gccgagagca gcccgagatt
     2221 gtgccgtccg ctgtttaccc agatgcgttt gacgatcgtt ttccacgccg ccgccctcat
     2281 gggctccaat gtcccggggg cagcgcctcg gcgcgccgcg cgtccctgtt ctgacataga
     2341 catgtccact tcggttcatg gtgggaacgg ggagaagggc gaggcccctt ctcctgcgaa
     2401 ggttgccctc aggaaggttc ggaccggtcg atgagaccgc tgtcgagcaa cggggcggca
     2461 tcgatattct gcgcatccat cagcatcgcc aaccgttcga cggctgcaag gatcatggcc
     2521 tgttcccatt gcggcagctt tgcgaaccgc tcggtgaacg tggtctgcag aaggtcaggc
     2581 gcctccgcca gagcctcgac gcccggcggc aggggccgca gaatgaactg gcgcttgtcc
     2641 ttttcactgc gctgccgctg cacgaagccg agttgctgaa gccggtcgac gattgcggtg
     2701 atcgtggcct gactgaattt aagagcggtg gcaatggcgc tgggagtcgc cgaatcgcgc
     2761 gacccgatct cgcgcaggac aagccattgc gaaggcgtga gaccggtcgt cgcggcaagc
     2821 cggcggctgc cgatttccgt ggcgcgcagg acccggcgga gggcccgaag ggtgaggacg
     2881 gcaacttctg aactcatgat ggaaacatag ggaaaatggc ctttcctggc caatcccatc
     2941 ctttgcaaac caaagcaatt gataaactcc ttcatcgacc aaaggacaac ttcgtgcgcc
     3001 aaagggttga aaatcgagta taatgcggta tcttccgcct caagccaaga gaggggcgcc
     3061 tgaaatacga agaatatact tcgggagatg aattatcttt gagacacggt ccgacgcggc
     3121 ttcatcgccg cagcggaagc gcgcgcgcca tcgcgatggc gcgatagcgg agatccggct
     3181 gcgtcttccc acagcggttg acgggccctt agtcacgaaa cttgttgcaa gctgtcctcc
     3241 gctcgacgtc aattccgcct actgcaatct tttgcagtgc acgcattttg cgcagagctg
     3301 catcattgcg gaaaaagccg ggcaactgat gggctgggtg tcgggctatc gaccgccgtc
     3361 cgaaccagag agttttttcg tctggcaggt cgctgtcgcg cccacagcga gggggcaggg
     3421 actggccgga cacatgatcg aggccttgct cgcgcgcccg tcatctgcag aagtcaccca
     3481 tctcatcacc acggtgacga atgataatca ggcatcctgg gctcttttcg aggggctcgc
     3541 gaacagatgg cgcaccaggc tcgagaggag cccattcttc caccagcaaa cgcatttcgc
     3601 cggcgcgcat gccaccgaat ggctggcgcg catcggcccg ctcccgcgct gaccgcgcgg
     3661 ccatccctca aggagacttg gcgacatgat cacccttccc cggcccacgg gccgcaaacc
     3721 cgataccgcc atctatgagc ggcgcgaatc cgccgtccgc agctatgcgc gcgtcatgcc
     3781 ccggcaattc aatcgcgccg agaatgtgtg gatgcacgac aatcagggtg gccgttatct
     3841 cgatttcctg tccggatgct cgacgttgaa ctatggtcac aatcatccag tgctcaagca
     3901 ggcgctgctc gactatatcg tggccgacgg gatcacgcat ggcctcgacc tgcacaccga
     3961 cgccaaggcg gaatttctgg aaacgctgga aaacctgatc ctcaaacccc gccgcctcga
     4021 ctatcgcgcg atgttcaccg gcccgaccgg cactaacgcg gtggaagccg cgatcaagct
     4081 cgcccgcaaa gttaccgggc gcgaactggt gatcgccttc accaacggct ttcacggcat
     4141 gacgctgggc gcactcgcct gcaccggcaa tgcggccaag cgcggcggcg cgggcgtacc
     4201 gctcgctcat gtcgcgcatg aaccctatga cggctatcac ggaccggagg tagacaccgc
     4261 cgagttgctg gagcggcggc ttgccgatcc ttcgagcggc cttgacgccc ccgccgccat
     4321 actggtggaa accgtgcagg gtgaaggcgg cctcaacgca gcatcacccg aatggctgcg
     4381 caggatcaat accatggcca agcggcacgg cgcgctgatg atcgtggacg acattcaggc
     4441 gggttgcggc cgtacgggaa gctttttcag cttcgagggc atgggcttca cccccgatat
     4501 cgtcaccatg gccaagtcgc tatcaggcat gggcctgccc ttcgcgctca cgctgttccg
     4561 gcctgaactg gatcaatggg cgccggggga acataacggc actttccgcg gcaataacca
     4621 tgccttcgtc actgccaccg cggcgctgcg ccatttctgg agcgaccggc gatttgagca
     4681 ggacatcgcc cgtcgcggcg cgctcattgc ccaacggctg gaggcgatgg cggcgcagca
     4741 tggcctgtcc acccgcggcc ggggcatgat gcgcgggatc gatgtcgggt cgggcgatat
     4801 cgccgccgcc gtcaccaccg cctgcttcga acggggcctt atcatcgaaa ccagcggggc
     4861 gcatgacgaa atcgtcaaga tcctttcccc cctggtcata gacgacgccg agctttcggc
     4921 cggcatcgac atattggaaa cgtgcatagg tgaagcattg ggcgctccct acggcgttgc
     4981 ggcggaataa aggagatatc atgatcgttc gcaaactgca ggacatccgt aaatcggacc
     5041 gcaatgtgaa gtcgcaacag tgggaaagcg cgcgcttgct gctgaaggac gataatatgg
     5101 gcttttcctt ccacatcacg accatgtatg cgggtgagga aatccacatg cattaccaga
     5161 accatctgga agcggtgctg gtgctgaagg gaaatggcac catcgaggat ctgggcacgg
     5221 gcatcacgca ccagcttgcg tcgggcgtca tgtatgcgct caacgcgcat gacaggcaca
     5281 ttgtgcgtcc gacgaccgac atcctgtgcg cctgcgtatt caacccgccg gtcaccggta
     5341 gggaagtaca tgacgagaac ggcgcctacc cggccgaagc cgacatgatc cgcgcggctg
     5401 cactgagtaa ttgaccgaag acgaaagggg gaagtccctt gcaagacatc tacccatccc
     5461 gccatgccaa gatggcggag tttctgccgc gcctcgatcc ggtcgttcac agcgagtgga
     5521 gcaaggacgc gccgatcggc agggacgagg ccgcgcagtt cgagcgcgat ggctatctgg
     5581 tgctggaaaa tctgttttcc gacaaggaga tcgcattcct ccagagcgag gcgggccagc
     5641 ttttgtcgga cccggacgcg ctggagggcg aaacggtgat cagcgagccg gacagccgcg
     5701 aaatccgttc gatcttccgc attcacgcgc aaagcccggt cattgcgaga ctggccgccg
     5761 acagcaggct ggcgggcgtc gcacgctttc tgttgggaga cgaggtctac atccatcagt
     5821 cgcgcctcaa ctacaagccc ggcttccagg gcaaggaatt ctactggcac agcgatttcg
     5881 agacctggca tgtcgaggat gggatgccgc gcatgcgggc gctgtccatg tcggtattgc
     5941 tggcggaaaa catggcccat aacgggccgc tgatgctgat ccccggatcg catcgcagct
     6001 tcctgacctg cgtcgggcaa acgccggagg atcattaccg catgtcgctc aaacggcagg
     6061 aatatggcgt gccggacgag gacagcctgg ccgaactggc gcacaggcat ggcatcgtcg
     6121 cgccgacggg tccgccaggg tcggtcgtga ttttcgactg caacatcatg cacgggtcga
     6181 acggcaatat cacccccttc ccccgggcca acgcctttct ggtctataat gccctcagca
     6241 accggctctg tgcacccttc ggggtcgaaa agccgcgtcc gcaattcatc gcagcgcgag
     6301 gcgagctgca gccgatcgtg ccggtatcag gctctatgac cgaaaggacg ggcatgccag
     6361 gccaggccgc ttaagacata atgttcttgg ccatcaaacc cgtccgcagc attttgctgt
     6421 ccatcttcat gctgatggcg ggcagcggct ttctggcgac gctgatcagc ctgcggctga
     6481 atcgggccgg cagcggcacg atgacgatcg gcatcgtcgc caccgcttat ttcggcgggc
     6541 tgatcatcgg gtcgctgcgc gcgggaggcg tggtccggag ggtgggacac atccgcgcct
     6601 tcgctgcctt cgtcgcgctg ctgtcggcga gtaccctgtc ctatgtgctg ctgtcgcatc
     6661 cgctgttctg gatgctgctg cggctggtcg acgggatgtg cgtcgcgggc gtgttcgtgt
     6721 gccttgaaag ctggcttaac gaccgggcgg agccgcaaat gcgcggcagc gtgctggccg
     6781 cctatatggt ggcgctctac tccggccagg ctatcggcca attgctgctg ggggcaagcg
     6841 gcgccctgcc ggccattcca ttccagcttg cctccatcct catttcgctg gcaatcatcc
     6901 cgctgtgcct cacgcgcagt gccgcacctg cgccagtgga ggccagcgcc ttttcgatcc
     6961 ggtcgctgct ggcggcctcg ccgctcggcg cctggggcgc tgtcgcgacg ggcctgatgc
     7021 taggcgcatt ttatgggctg gcggccgtgc atgtgagacg gctgggtctg gatctgcccg
     7081 aaaccgccag gttcatgatg ttcgtcatcc tgggcggcgt ggcgctgcaa tggcctttgg
     7141 gccggctgtc ggatcgacat gaccgcaggc aggtgatcat cggcagcttc gcggcggcgg
     7201 cgctggtcag catggcgctg tcgctcggcc aggatggcct gtggctgacg ggcctcggcg
     7261 cgctgttcgg gggattgagc ttcgcgctct atccgctgtg cgtggcccat tgcaacgacc
     7321 ggctgctgga gaccgagcgc gtggcggcaa gcggacggct ggtgcttctc tattccctcg
     7381 gcgcagcgct cgggccgctg ctggcagccg gcgccatgac cgcttttgga accggcgggt
     7441 tgtttctctt catcgcgctg tgcgccgggc tcgccatgac ggtcggcttg tggcgccagc
     7501 gcgcgtccga gccggtgccg gttagatatc agcaggattt ccaggtcctg ccccgcacca
     7561 cgcccatggc ttcgctgctc gatcccaaca gcgcagacga gacgctgacc caaggacaga
     7621 cgacatgacc gcgatttcca gtcacaggac ggtagccgcc tcccccacga cggcgggcga
     7681 cgtcacgttg cgccgcgcca tcgccgcgtc ggccatgggc aatgcgaccg aatggttcga
     7741 ctatggcatc tatgcctatg gggtcaccta tatttccgca gcgctcttcc cgggccccac
     7801 gnnnnnnnnn nnnnnnnnnn ncgctgccct gctgatgttg cccgacgacg aggcggcgat
     7861 cgtgaagcac tatagtctgt caggtgagga catgaccgcg atcgacaccg cgcgcacgcc
     7921 cgccacccga ctcggctacg ccctgcagct ttgttgcctg cgctacccgg gccggcatct
     7981 gcgccacggg gaactgctgc ctgcggtcat gctcgaccat attgccgagc aggtcggggt
     8041 ggatgcaaag gtcatagctg atttcgccag acgcacgccc acgcgctacg atcagctcgc
     8101 cgcgatcaag acgcgcttcg gcttcagcga tctgagccgc ccgcaccgcg tggaattgcg
     8161 gacatggctg acaaacgagg cggcgagcat catcgacgga cgcgcgctgc tcggccggct
     8221 tctggatgag atgcgcgcgc gtcggatcgt gatcccgggt gtcagcgtcg tggagcgcat
     8281 ggcggccgag gcgatacatc aggcggaaac ggatctcgtg gccgcgatcg atggcggcct
     8341 tggccatgag atgcgtcagc aactcgacgc gctgatcgat gacaaggtgc atgaccggca
     8401 gagccgtctg tcctggctgc gcgagccaga accgcgtgtc gcatccgctt cgcttttgga
     8461 aatcgtcgaa aagatcacgc tgattcgtgg gacgggcata tcggctttct cccctgatgt
     8521 tcgccacgaa ccgcgcctgg ggcaattcgc ccgggagggt gtgcgctaca cggcacaggc
     8581 tttccagcaa atgcgcccgg cccgcaggcg ggtcgtgctg ctcgcgacgc tgcgcgagct
     8641 ggaggcgaca ctgaccgatg cggcgatcga tatgttcatt gcgctcgtgg ggcgggccca
     8701 tctgcgcgcc cgcaaacggc tcgaacagcg cgtggccgtt tccggccgcg agggccgcga
     8761 acggatgtta cgcatcgcca gggttctgga agcgatcagc caggcggccc gggctggcgg
     8821 cgatgtcgcc gcggcggtcg atgccgtggc atccctcgac atcatcgacg ccgacgccgc
     8881 tatcatccga cgtaccgcct caccgcaccg gaacgaggtt ctggacgaga tcgcggcgga
     8941 atatcgcgcc ttcaagcgga tggggccgtc gttcgtgcgc gcttttgatt tccagggccg
     9001 ggccggaatg cagccgttgc gcgatgcgat ggcgatcctc gccgatctcg acggagactg
     9061 gcgcagggcg ttgcccgatg acgtgccgct tggccatgtc gagcaccgct ggcgtcggca
     9121 tgtcatgacc gcaggcggga tcgatcgcac ccattgggag atggcgactt acagcgcgct
     9181 ttccaatgct ttggcatccg gtggtatctg ggtgccgacc gcgcgcgtcc atcgcgcgct
     9241 cagtgtgctg cttgccccac cggccagccc cgtaccgaag cccgctttct cgctcggcga
     9301 tccgcacgcc tggctcgacg agagggcggc aaggcttgat agcgccctgc gcgaggtcgc
     9361 ccgcgatctg gacaaacgcg atccccccct ttttgccggc gagcggctgc ggtttccgaa
     9421 agaccccaaa gaagaccctg gtcaggatga agggcggcaa cttgcgctca cctgctacgg
     9481 catggtgcct gccacccgta tcacggatgt gctgtcgcag gttcaacgat ggaccggctt
     9541 catccagcat ttcggccatg tctcgaccgg tctgcctccc gccgacgaac gcgcctttct
     9601 tgccaccttg atcgcggagg cgaccaacct tggtctgtcg cgcatggccg aagtttgcgg
     9661 tgtcgcctcg cgtcgcgcac tgttgcgtat gcagacgtgg catatgcgcg aggaaacgtt
     9721 tcgcgccgcg ctcgccagcc tgaccgacgc tatccatgcc gaaccgctcg ccgcctggtt
     9781 tggttcgggg catcgcgcat cggccgatgg gcaggcttat tatctcggcg gtgccggcga
     9841 agccggagga acggtcaacg cacattacgg tcgcgacccc gtggtcaaga tctacaccac
     9901 catcaccgat cgatacgcac cgcttcatca gacggtcatc gccgggacgg cgggcgaggc
     9961 gatccacgca ctcgacggca tcctcggcca cgaaagcagc gcggacatca ccgccctcca
    10021 caccgatggt ggcggcgtct ccgatatcgt gttcgccgtc atgcatttgc tcggcctcga
    10081 tttcgagccg cgcattccgc gcctgtcgga tcgacaactc tatggtttcg agcccgcgcg
    10141 gcgctatggt cggctcgcac cgctgttcgg gcgccgcctc ggtcgggacc tgatcgtcag
    10201 ccattgggcc gaaattgcgg aggtcatcgc ggcgatgcgc gaccgcactg tcacaccgtc
    10261 gctgattttg aagaagctgt ccgcctatcg tcaacaaaac agtcttgctg cggcacttcg
    10321 ggaggtcgga cggatcgagc gcacactgtt cacgctgcgc tggttcgacg acaccgatct
    10381 gcgccggacg gtcaccgccg aactcaacaa gggc
//
