LOCUS       NZ_NERK01000015         5389 bp    DNA     linear   CON 23-AUG-2024
DEFINITION  Bacillus halotolerans strain RSCu-8D Scaffold_15, whole genome
            shotgun sequence.
ACCESSION   NZ_NERK01000015
VERSION     NZ_NERK01000015.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus halotolerans
  ORGANISM  Bacillus halotolerans
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 5389)
  AUTHORS   Gajera,H.P., Hirpara,D.G., Katakpara,Z.A., Kothari,V. and
            Golakiya,B.A.
  TITLE     Genome sequence of Bacillus halotolerans RSCu-8D
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5389)
  AUTHORS   Gajera,H.P., Hirpara,D.G., Katakpara,Z.A., Kothari,V. and
            Golakiya,B.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2017) Department of Biotechnology, Junagadh
            Agricultural University, Motibag, Junagadh, Gujarat 362 001, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-OCT-2016
            Assembly Method                   :: SPAdes v. 8.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 84.0x
            Sequencing Technology             :: IonTorrent
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002592025.1-RS_2024_08_23
            Annotation Date                   :: 08/23/2024 17:34:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,284
            CDSs (total)                      :: 4,199
            Genes (coding)                    :: 4,047
            CDSs (with protein)               :: 4,047
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 152
            CDSs (without protein)            :: 152
            Pseudo Genes (ambiguous residues) :: 0 of 152
            Pseudo Genes (frameshifted)       :: 101 of 152
            Pseudo Genes (incomplete)         :: 85 of 152
            Pseudo Genes (internal stop)      :: 26 of 152
            Pseudo Genes (multiple problems)  :: 53 of 152
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:21:27
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 5389
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NERK01000015.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5389
                     /collected_by="Darshana G. Hirpara"
                     /collection_date="2015-10-10"
                     /db_xref="taxon:260554"
                     /geo_loc_name="India: Gujarat, Jasdan"
                     /host="Cuminum cyminum"
                     /lat_lon="21.52 N 70.45 E"
                     /mol_type="genomic DNA"
                     /organism="Bacillus halotolerans"
                     /strain="RSCu-8D"
                     /submitter_seqid="Scaffold_15"
     region          1..5389
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="transAT-PKS-like"
                     /region_number="1"
                     /rules="cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS
                     or itr_KS or tra_KS) and not PKS_AT)"
                     /tool="antismash"
     cand_cluster    1..5389
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(ATd and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS) and not PKS_AT)"
                     /kind="single"
                     /product="transAT-PKS-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..5389
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[70:4750](+)"
                     /cutoff="45000"
                     /detection_rule="cds(ATd and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS) and not PKS_AT)"
                     /neighbourhood="20000"
                     /product="transAT-PKS-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      71..4750
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="45000"
                     /detection_rule="cds(ATd and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS) and not PKS_AT)"
                     /neighbourhood="20000"
                     /product="transAT-PKS-like"
                     /protocluster_number="1"
     gene            71..4750
                     /locus_tag="B9T64_RS12855"
                     /old_locus_tag="B9T64_12855"
     CDS             71..4750
                     /NRPS_PKS="Domain: Trans-AT_docking (1-157). E-value:
                     4.7e-29. Score: 93.7. Matches aSDomain:
                     nrpspksdomains_B9T64_RS12855_Trans-AT_docking.1"
                     /NRPS_PKS="Domain: PKS_DH2 (193-421). E-value: 1.4e-52.
                     Score: 170.3. Matches aSDomain:
                     nrpspksdomains_B9T64_RS12855_PKS_DH2.1"
                     /NRPS_PKS="Domain: PKS_KR (713-893). E-value: 4.6e-35.
                     Score: 113.1. Matches aSDomain:
                     nrpspksdomains_B9T64_RS12855_PKS_KR.1"
                     /NRPS_PKS="Domain: PP-binding (983-1049). E-value: 1.1e-09.
                     Score: 30.5. Matches aSDomain:
                     nrpspksdomains_B9T64_RS12855_PP-binding.1"
                     /NRPS_PKS="Domain: PKS_KS(Trans-AT-KS) (1118-1555).
                     E-value: 5.4e-171. Score: 561.1. Matches aSDomain:
                     nrpspksdomains_B9T64_RS12855_PKS_KS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     transAT-PKS-like: ATd"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     transAT-PKS-like: tra_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022: Beta-ketoacyl synthase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012337.5"
                     /locus_tag="B9T64_RS12855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9T64_12855"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_371411782.1"
                     /sec_met_domain="ATd (E-value: 1.6e-30, bitscore: 93.7,
                     seeds: 19, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short (E-value: 1.9e-34, bitscore:
                     106.3, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 4.5e-10, bitscore:
                     27.2, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="tra_KS (E-value: 3.6e-193, bitscore:
                     629.2, seeds: 44, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 6.8e-88, bitscore:
                     281.5, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLVLSAKTPEALQGKIHEMAGFLEKSQNSGAMDLAKISYTLMEGR
                     HHFDCRCAVVIKDTEDAVRVLRQAEGNEKLPNLFKRTVSRDFTARAVLRKAMKDIIKQN
                     YMEENPNKYKENLCALAEFYCLGYEVSTDLFGDVKFNKVNLPSYPFAKQSHWIEKAEVF
                     QPYSKRDTGIIHPFLHQNVSSLLGQRYRTGFTGHEFFIRDFTVKGQRLLPDSIYLEMAR
                     MAFADAGREIVAETNGILIQNIEWMNPISDELQQLELFTGLYPEADGPIIYEIYSKSES
                     NPVVHCRGTGKFVQPDRSGLIDIRAIKDKCNSNYVSSNKWNRVLHVAGIKNGAGFNALK
                     KLYIGQKSVLAELELPSCVNDSKGSFMLHPSMLEAALQSVMGLTLKQNQKDEIIAVKKP
                     LSMALGELEVINACTEKMWAYVTLNDEGILDSTQALFQQVNITLCNHEGDIIIKLTDYS
                     YQLSEEYQINILEKGNGSSDSLQGNNQSIAIKDRVNEITEENITMIPVWKRIVLANKQK
                     TVNHMLIAGDFSEGRTELLREYPNASVWNTQDKEWSIDKIAGKLKKNGEIDHLVWIVPA
                     SEIYSVVSQEVVDSQEYGVIFLFKMIKALLQLEYHNKEFDLTVITSNSQAVLDDDIVNP
                     VHAGINGIVGSVAKEFPQWNIRLVDLENPVHWPVNTMLHIPVDKEGNAVAYRKNSWYKM
                     QLVSYSRKNSSVQSVYREGGVYVVIGGAGGIGETWSEYMIRHYKANIIWIGRRMRNTEI
                     DKKIERLSAFGPAPRYISADATDVESLERAYKQIKKIYPHVNGLVHSAIGLLDRSISEL
                     DVDTYKKIIGIKINASVYMAKVFANESLDFVVYFSSVASFNKLGGQSGYSTGCTFEDGF
                     VQRLSKEWSCKVKTINWGYWGDIGIGESLSEAFKIRISMSGMSPINPKKAMEDLDFIMS
                     LPECQLLYMRGDKSKENSMVANGMPSKSQAIPIQEPKRRESTQIENLLLEKSTQYIAKL
                     VGQSLKIPYHQLDTREDLEKYGIDSIMVKHLTYTFNQVFNNIYSTLFFECKSIDELVHY
                     LIRNQKDTLLNLFNLNEDQSMSVEEGDKNNSISTRKTDSMFDGSRTDRRISNLLDQDNQ
                     VNKHGTSNSMPIAIIGMSARLPQANDLDEFWENLRAGKDSITEIPEERWSLDGFYISDV
                     KKAIEQGKSYSKWGGFIDGFADFDPLFFKISPREAMTMDPQERLILQECWKTFEDAGYT
                     KERIKQHHNGNVGVFIGITKTGFELYGPDLWKQGENINLSTSFSSVANRISYIFDLHGP
                     SLPIDTMCSSSLSAIHEACETIKRGDCEVAIAGGVNVYTHPATYILLCEKKMLSSDGKC
                     KSFGKGANGFVPGEGAGAILLKPLAKAIEDGDNIYGVIKGTSINHGGKTNGYTVPNPNA
                     QGELIRNALDKSGVNARTVSYIEAHGTGTELGDPIEIAGLTQAFQSDTKDLQFCSIGSA
                     KSNIGHLESAAGIAGLMKIVLQMKNGQIAPSLHAEELNSHIDFEDSPFYVQKELVDWKR
                     PVIEHNGTSQEYPRISGVSSFGAGGANAHVILEEYIKGK"
     aSDomain        74..541
                     /aSDomain="Trans-AT_docking"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B9T64_RS12855_Trans-AT_docking.1
                     "
                     /evalue="4.70E-29"
                     /label="B9T64_RS12855_Trans-AT_docking.1"
                     /locus_tag="B9T64_RS12855"
                     /protein_end="157"
                     /protein_start="1"
                     /score="93.7"
                     /tool="antismash"
                     /translation="LVLSAKTPEALQGKIHEMAGFLEKSQNSGAMDLAKISYTLMEGRH
                     HFDCRCAVVIKDTEDAVRVLRQAEGNEKLPNLFKRTVSRDFTARAVLRKAMKDIIKQNY
                     MEENPNKYKENLCALAEFYCLGYEVSTDLFGDVKFNKVNLPSYPFAKQSHWI"
     aSModule        74..3217
                     /domains="nrpspksdomains_B9T64_RS12855_Trans-AT_docking.1"
                     /domains="nrpspksdomains_B9T64_RS12855_PKS_DH2.1"
                     /domains="nrpspksdomains_B9T64_RS12855_PKS_KR.1"
                     /domains="nrpspksdomains_B9T64_RS12855_PP-binding.1"
                     /incomplete
                     /locus_tags="B9T64_RS12855"
                     /tool="antismash"
                     /type="pks"
     aSDomain        650..1333
                     /aSDomain="PKS_DH2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B9T64_RS12855_PKS_DH2.1"
                     /evalue="1.40E-52"
                     /label="B9T64_RS12855_PKS_DH2.1"
                     /locus_tag="B9T64_RS12855"
                     /protein_end="421"
                     /protein_start="193"
                     /score="170.3"
                     /tool="antismash"
                     /translation="FTGHEFFIRDFTVKGQRLLPDSIYLEMARMAFADAGREIVAETNG
                     ILIQNIEWMNPISDELQQLELFTGLYPEADGPIIYEIYSKSESNPVVHCRGTGKFVQPD
                     RSGLIDIRAIKDKCNSNYVSSNKWNRVLHVAGIKNGAGFNALKKLYIGQKSVLAELELP
                     SCVNDSKGSFMLHPSMLEAALQSVMGLTLKQNQKDEIIAVKKPLSMALGELEVINACTE
                     KMWAYV"
     aSDomain        2210..2749
                     /ASF="KR domain putatively catalyzing D-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N found: False"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B9T64_RS12855_PKS_KR.1"
                     /evalue="4.60E-35"
                     /label="B9T64_RS12855_PKS_KR.1"
                     /locus_tag="B9T64_RS12855"
                     /protein_end="893"
                     /protein_start="713"
                     /score="113.1"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="GVYVVIGGAGGIGETWSEYMIRHYKANIIWIGRRMRNTEIDKKIE
                     RLSAFGPAPRYISADATDVESLERAYKQIKKIYPHVNGLVHSAIGLLDRSISELDVDTY
                     KKIIGIKINASVYMAKVFANESLDFVVYFSSVASFNKLGGQSGYSTGCTFEDGFVQRLS
                     KEWSCKVKTINWGYWGD"
     CDS_motif       2213..2311
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B9T64_RS12855_0001"
                     /evalue="1.90E-10"
                     /label="PKSI-KR_m1"
                     /locus_tag="B9T64_RS12855"
                     /protein_end="747"
                     /protein_start="714"
                     /score="33.4"
                     /tool="antismash"
                     /translation="VYVVIGGAGGIGETWSEYMIRHYKANIIWIGRR"
     CDS_motif       2588..2692
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B9T64_RS12855_0002"
                     /evalue="4.70E-10"
                     /label="PKSI-KR_m4"
                     /locus_tag="B9T64_RS12855"
                     /protein_end="874"
                     /protein_start="839"
                     /score="32.0"
                     /tool="antismash"
                     /translation="LDFVVYFSSVASFNKLGGQSGYSTGCTFEDGFVQR"
     aSDomain        3020..3217
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B9T64_RS12855_PP-binding.1"
                     /evalue="1.10E-09"
                     /label="B9T64_RS12855_PP-binding.1"
                     /locus_tag="B9T64_RS12855"
                     /protein_end="1049"
                     /protein_start="983"
                     /score="30.5"
                     /tool="antismash"
                     /translation="QYIAKLVGQSLKIPYHQLDTREDLEKYGIDSIMVKHLTYTFNQVF
                     NNIYSTLFFECKSIDELVHYL"
     aSDomain        3425..4735
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B9T64_RS12855_PKS_KS.1"
                     /domain_subtypes="Trans-AT-KS"
                     /domain_subtypes="beta-D-OH"
                     /evalue="5.40E-171"
                     /label="B9T64_RS12855_PKS_KS.1"
                     /locus_tag="B9T64_RS12855"
                     /protein_end="1555"
                     /protein_start="1118"
                     /score="561.1"
                     /specificity="transATor: beta-D-OH"
                     /tool="antismash"
                     /translation="IAIIGMSARLPQANDLDEFWENLRAGKDSITEIPEERWSLDGFYI
                     SDVKKAIEQGKSYSKWGGFIDGFADFDPLFFKISPREAMTMDPQERLILQECWKTFEDA
                     GYTKERIKQHHNGNVGVFIGITKTGFELYGPDLWKQGENINLSTSFSSVANRISYIFDL
                     HGPSLPIDTMCSSSLSAIHEACETIKRGDCEVAIAGGVNVYTHPATYILLCEKKMLSSD
                     GKCKSFGKGANGFVPGEGAGAILLKPLAKAIEDGDNIYGVIKGTSINHGGKTNGYTVPN
                     PNAQGELIRNALDKSGVNARTVSYIEAHGTGTELGDPIEIAGLTQAFQSDTKDLQFCSI
                     GSAKSNIGHLESAAGIAGLMKIVLQMKNGQIAPSLHAEELNSHIDFEDSPFYVQKELVD
                     WKRPVIEHNGTSQEYPRISGVSSFGAGGANAHVILEEY"
     CDS_motif       3917..3964
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B9T64_RS12855_0003"
                     /evalue="7.50E-05"
                     /label="PKSI-KS_m3"
                     /locus_tag="B9T64_RS12855"
                     /protein_end="1298"
                     /protein_start="1282"
                     /score="15.1"
                     /tool="antismash"
                     /translation="GPSLPIDTMCSSSLSA"
     CDS_motif       4337..4384
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B9T64_RS12855_0004"
                     /evalue="1.10E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="B9T64_RS12855"
                     /protein_end="1438"
                     /protein_start="1422"
                     /score="14.1"
                     /tool="antismash"
                     /translation="YIEAHGTGTELGDPIE"
     CDS_motif       4445..4489
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B9T64_RS12855_0005"
                     /evalue="1.60E-03"
                     /label="PKSI-KS_m6"
                     /locus_tag="B9T64_RS12855"
                     /protein_end="1473"
                     /protein_start="1458"
                     /score="11.1"
                     /tool="antismash"
                     /translation="GSAKSNIGHLESAAG"
     gene            4761..>5389
                     /locus_tag="B9T64_RS12860"
                     /old_locus_tag="B9T64_12860"
     CDS             4761..>5389
                     /NRPS_PKS="Domain: Trans-AT_docking (10-167). E-value:
                     7.6e-54. Score: 174.2. Matches aSDomain:
                     nrpspksdomains_B9T64_RS12860_Trans-AT_docking.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATd"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF046639.1"
                     /locus_tag="B9T64_RS12860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9T64_12860"
                     /product="hypothetical protein"
                     /protein_id="WP_180237060.1"
                     /sec_met_domain="ATd (E-value: 2.5e-55, bitscore: 174.2,
                     seeds: 19, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGGIDEMLKQPEIIVLSARDKIRLRDQVQRLATTIDKRKFTDADL
                     TNISYTLLVGREHMEYRLALLVTSIKELEEKLYSYIAGEEATIDFFQGAAHGNDDILSV
                     FGKEEELQTAIEKLLENKKYERILDFWTKGISIDWNKLFDQMAVRPHFISLPTYPFAKE
                     KYWVPSEIKQPSAVSTNQLGIIHPLVHENTSDLSRQRFTSALTGKE"
     aSDomain        4791..5261
                     /aSDomain="Trans-AT_docking"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B9T64_RS12860_Trans-AT_docking.1
                     "
                     /evalue="7.60E-54"
                     /label="B9T64_RS12860_Trans-AT_docking.1"
                     /locus_tag="B9T64_RS12860"
                     /protein_end="167"
                     /protein_start="10"
                     /score="174.2"
                     /tool="antismash"
                     /translation="PEIIVLSARDKIRLRDQVQRLATTIDKRKFTDADLTNISYTLLVG
                     REHMEYRLALLVTSIKELEEKLYSYIAGEEATIDFFQGAAHGNDDILSVFGKEEELQTA
                     IEKLLENKKYERILDFWTKGISIDWNKLFDQMAVRPHFISLPTYPFAKEKYWV"
     misc_feature    complement(4807..4822)
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 20.43"
                     /tool="antismash"
ORIGIN
        1 gcttcaaagc tatacgccag aaagtactgt aagagagaag gaaaacactc agaacgacaa
       61 accatattat ttgcttgttt tatcggccaa aacacctgaa gccttgcagg ggaaaattca
      121 tgagatggcg ggttttcttg aaaaatctca aaatagtgga gcgatggacc tggcaaaaat
      181 cagctatacg ctgatggaag gaagacatca cttcgactgc cgctgtgcgg ttgtgatcaa
      241 agacacggaa gatgcggtcc gagtactcag acaagctgaa gggaacgaaa aactgccgaa
      301 tttgtttaaa aggacagtct caagagactt tacagcccgg gcagtactta ggaaagcgat
      361 gaaggatatc ataaagcaaa attatatgga agaaaatccg aataagtata aagaaaatct
      421 gtgtgcgttg gccgagtttt attgtctagg ttatgaagta tctacagatc tttttggcga
      481 tgtaaagttt aacaaagtca atcttccttc atatcctttt gcgaagcaga gtcactggat
      541 agaaaaggcg gaggtttttc aaccttacag caagcgtgac acaggaatta ttcacccgtt
      601 cctgcatcaa aatgtctcat ccttattagg acaacgttac cgtactggtt ttacaggtca
      661 tgagtttttt ataagagatt ttactgttaa agggcagcgc ttattacctg attcaattta
      721 cctggaaatg gcgagaatgg catttgctga tgcaggcagg gagattgtcg cagaaactaa
      781 tgggatctta atccaaaata tagagtggat gaaccctata tcagatgaat tgcaacagtt
      841 ggaactgttt accgggttat atccagaagc cgatggaccg attatttatg aaatctatag
      901 taaaagtgaa agtaatccgg ttgtacattg tcgaggtaca ggaaaatttg tacagcctga
      961 cagatctggt ttaatagata taagagcaat aaaagataaa tgtaattcaa actatgtaag
     1021 ctcgaataaa tggaatcgag ttttacatgt agcagggatc aaaaatggcg cgggatttaa
     1081 tgcactaaag aaactttata ttggacagaa aagtgtatta gctgaattgg aattgccaag
     1141 ttgcgtaaat gacagcaaag gttcctttat gcttcatcca agtatgcttg aggcggcttt
     1201 acaatcggta atggggctaa cgctcaagca gaatcaaaag gatgaaatca ttgcagttaa
     1261 aaaaccttta agtatggctc ttggagaatt agaagtcata aatgcctgca ccgaaaaaat
     1321 gtgggcttat gtaaccttaa atgatgaagg aatacttgat tctacacagg ctttatttca
     1381 acaagtgaat attactttat gtaatcatga aggagatatc attataaaat taacagatta
     1441 ctcctatcaa ttatcagaag aatatcaaat caacatctta gagaaaggga atggatccag
     1501 cgatagtctg caaggaaata atcaatctat tgccattaaa gatagagtca atgaaattac
     1561 agaagaaaac attacaatga ttcctgtatg gaagcgaatt gtattagcaa ataaacaaaa
     1621 aacagtaaac catatgctga ttgccggtga tttcagtgaa ggaagaactg agttattaag
     1681 ggaatatcca aatgcgtccg tatggaatac tcaggataaa gagtggagca tcgataaaat
     1741 tgctggaaaa ctgaagaaaa acggtgaaat tgaccacctt gtgtggatag tgcctgccag
     1801 cgaaatatat tcagtagtta gtcaagaggt cgttgactct caagaatatg gagtaatttt
     1861 cttatttaaa atgataaagg cccttcttca gcttgaatat cataataaag aatttgattt
     1921 aactgttatt acctctaaca gtcaggctgt attggatgat gacattgtaa atcctgtgca
     1981 tgctgggatt aatgggatag ttggttctgt agctaaagaa tttcctcaat ggaacattcg
     2041 tcttgttgat ttagaaaatc cagtgcactg gcctgtaaat acaatgttac atatacctgt
     2101 agataaagaa ggaaacgcag ttgcatatag aaagaacagc tggtacaaaa tgcagcttgt
     2161 tagctacagc aggaagaact cctcggtaca aagtgtttat cgagaaggcg gagtctatgt
     2221 tgttatcggc ggagccggag gtatcggaga aacctggagt gagtacatga ttcgtcatta
     2281 taaggcgaat atcatctgga ttggtcgaag aatgaggaat accgaaatcg ataaaaaaat
     2341 tgaaagactc agtgcttttg gtccggcacc gcgctacatt tcagcagacg ctactgatgt
     2401 agagtcactg gaaagagcat acaaacaaat taaaaaaata tatccacatg ttaacggcct
     2461 tgttcattca gctattggct tgcttgacag aagtatatct gaattggacg tagacactta
     2521 taaaaaaatc ataggaataa agataaatgc cagcgtatat atggcaaaag tttttgcgaa
     2581 tgaatctctt gattttgttg tctatttttc atctgttgcc tcctttaata aactaggcgg
     2641 acaaagcggg tattctacag gttgtacatt tgaggatgga tttgttcaaa gactatcaaa
     2701 agaatggtca tgtaaagtta aaacaattaa ttggggatat tggggagaca tcggtattgg
     2761 cgagtcactt tctgaagcgt ttaagatcag aatttctatg tctggaatga gtccgattaa
     2821 tcctaaaaaa gctatggagg atttggactt tattatgtct ttacctgaat gtcagctcct
     2881 ttatatgaga ggcgacaaat caaaagaaaa ttcaatggta gcaaacggta tgccgtctaa
     2941 gtcacaggct attcccattc aggaacctaa gagaagagag agcactcaaa ttgaaaactt
     3001 gttattagaa aagagcaccc agtatatagc caaattagtg ggacagtctc tcaagatccc
     3061 gtaccaccag ttggatacaa gagaagacct tgaaaagtat ggtattgatt cgattatggt
     3121 caagcattta acttatacat ttaatcaagt tttcaacaat atatacagta ctctgttctt
     3181 tgaatgcaag agtattgatg aattagtaca ttacttgatt agaaaccaaa aggataccct
     3241 tctgaatctc tttaatttaa atgaagatca aagtatgagt gtggaagagg gggataaaaa
     3301 taatagtatt tcaacccgta aaacggattc aatgtttgac ggaagcagga ctgacaggcg
     3361 gataagtaat ttgctagatc aagataatca ggtgaacaaa catggaacat caaattctat
     3421 gccaattgcg ataataggaa tgagcgcacg attaccgcag gcgaatgatc ttgatgagtt
     3481 ctgggaaaat ctaagggctg gtaaagacag cattacggaa attccagagg aacgatggtc
     3541 tttagatggc ttttatatta gtgatgtaaa aaaagctatc gaacagggca agagctatag
     3601 taagtggggc ggatttatcg atggctttgc cgactttgac cctctttttt ttaagatctc
     3661 gccacgtgaa gcgatgacga tggacccaca ggaacgcttg atcttgcagg agtgctggaa
     3721 aacttttgaa gatgcaggct ataccaagga gaggattaag cagcatcaca atggtaatgt
     3781 tggtgttttt ataggcatca caaaaactgg atttgagtta tacggtccag atttatggaa
     3841 gcaaggtgag aatattaatc tcagcacttc gtttagttca gttgcaaacc gtatttcata
     3901 tatttttgat ttgcacggtc cgagcctgcc tattgataca atgtgctctt catcattatc
     3961 agccatccat gaagcctgtg aaactattaa acggggcgat tgtgaagtag ccattgctgg
     4021 aggagtgaat gtatatactc acccagcaac ttatatttta ctctgtgaaa agaaaatgtt
     4081 gtcaagtgat ggcaaatgta agagctttgg caagggggca aatgggtttg taccgggcga
     4141 aggtgcagga gcgatccttt taaaacctct tgctaaagca atagaagacg gcgataacat
     4201 ttatggagtt atcaaaggta caagtattaa ccatggagga aaaacaaatg gttatacagt
     4261 gcctaatcca aacgcgcagg gcgaacttat tcgtaatgca ttggataaat caggagttaa
     4321 tgcacgtaca gttagctata ttgaagctca tggtacagga actgagcttg gagatcccat
     4381 tgagattgcg ggtcttacac aggcatttca aagtgataca aaagatttgc aattctgttc
     4441 tatcggttca gccaagtcca atattgggca ccttgaaagt gcagcaggaa ttgccggttt
     4501 aatgaaaatc gttcttcaaa tgaaaaatgg ccagattgca ccatccctac atgcagagga
     4561 attaaattct catattgatt ttgaagatag tccattttat gtacaaaagg aactggttga
     4621 ttggaagagg ccggtaattg aacataatgg cacaagtcaa gaatatccga gaatttctgg
     4681 cgtttcttca tttggtgctg ggggagctaa tgctcacgtt atattagaag aatatattaa
     4741 gggaaaataa ggggcttatt atggggggaa tcgacgaaat gttaaaacaa cctgaaatta
     4801 tcgtcttatc cgccagggat aaaatccgac tccgcgacca ggtgcaacgc ttggctacga
     4861 cgattgataa acggaagttt acggatgctg accttactaa tattagttat acgctcttag
     4921 ttggtcgtga acatatggaa tatcgcttag ctttattagt aacttctata aaagaacttg
     4981 aagaaaaact ttattcttat atagcaggcg aagaagccac cattgacttt ttccaggggg
     5041 ctgcccatgg aaatgatgat attttatctg tttttggtaa agaagaggaa ctgcagactg
     5101 caatagaaaa attgctcgaa aataaaaagt atgaaaggat tcttgatttt tggacgaagg
     5161 ggattagtat agattggaat aaactgtttg atcaaatggc agtcagacct cactttatta
     5221 gccttccgac ctatccgttt gcgaaggaaa aatactgggt gccttcagaa attaaacagc
     5281 cgtctgcagt atcaacgaac cagctcggta ttatacaccc gctggttcat gaaaatacat
     5341 ctgatcttag taggcagcgt ttcacttctg cattaactgg aaaagagga
//
